CN110003885A - A kind of preparation method and applications of Ratiometric fluorescent probe - Google Patents
A kind of preparation method and applications of Ratiometric fluorescent probe Download PDFInfo
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Abstract
The invention discloses a kind of Ratiometric fluorescent probe and its preparation and application, it is related to the analyzing detecting method of phytic acid, with ferric ion by carbon dots fluorescent quenching, then phytic acid sample is added, fluorescence is resumed, with the increase of sample to be tested concentration, the fluorescence of recovery grows from weak to strong, and realizes qualitative and quantitative detection.Dioxide composite nano silicon particles are to be formed by composite nanometer particle using covalent coupling carbon dots after coating cadmium selenide particle as kernel, its surface amination.For cadmium selenide inside nano SiO 2 particle core as reference fluorescent signal, the carbon dots of outer layer are used for ferric Selective recognition as corresponding fluorescence signal.The present invention realizes the Ratiometric fluorescent probe for the specific detection of phytic acid since ferric ion is for interaction strong between the fluorescent quenching effect of carbon dots and phytic acid and ferric ion using fluorescence " on-off " mode.
Description
Technical field
The present invention relates to a kind of preparation sides for ferric ion and the Ratiometric fluorescent probe of phytic acid quickly detected
Method and its application.
Background technique
Phytic acid (PA), also known as phytic acid exist extensive in the seed of the plants such as soybean and cereal.Phytic acid is in day
It often is typically used as antioxidant, chelating agent and antistaling agent etc. in life, and is being eaten with its unique structure and chemical property
It is used widely in the fields such as product, daily-use chemical industry, medicine and intermetallic composite coating and Polymeric Industry.In order to be further understood that it
Physiological action, the qualitative and quantitative analysis of phytic acid become particularly important.Currently, most common phytic acid detection method is chromatography
Method, but the deficiencies of this method is there are instrument price valuableness, and testing cost is high, and operator need to be through professional training, it is difficult to for existing
Field detecting.
Fluorescence analysis due to its is easy to operate, high sensitivity, good selective and there is significant detection advantage,
It is received significant attention in the fields such as food safety and environmental monitoring.But the fluorescence intensity of single fluorescence probe needs strict control to examine
Survey condition could obtain accurate testing result, and the stability of the variation of external environment, the concentration of detection substrate and instrument is equal
Testing result can be produced a very large impact.Therefore, the good novel fluorescence probe body of easy to operate, high sensitivity, stability is studied
System realizes that the quantitative analysis of phytic acid in sample is of great significance.
Compared with the fluorescence probe of the single transmitting of tradition, Ratiometric fluorescent probe passes through while detecting under same excitation wavelength
Fluorescence intensity at different wave length can overcome interference of the factors above to testing result.Currently, Ratiometric fluorescent probe has been answered
In detection for substances such as metal ion, pH value of solution, temperature and biological micromolecules.
Carbon dots are a kind of novel carbon nano-luminescent materials, not only have excellent optical property and small size property, together
When there is good biocompatibility and the performances such as green non-poisonous, answered extensively in the fields such as environment measuring and bio-sensing
With.The surface of carbon dots is modified using property different passivator, the performance of carbon dots can be changed, to widen its application
Range.Due to PA and Fe3+Between have very strong complexing power, designing one kind based on this can be highly sensitive, quick, inexpensive
And Selective recognition Fe3+Ratiometric fluorescent probe, while by fluorescence Restoration Mechanism detect PA, have highly important meaning
Justice.
Summary of the invention
It is an object of the present invention to provide a kind of preparation method of Ratiometric fluorescent probe,
It is another object of the present invention to provide above-mentioned Ratiometric fluorescent probes in ferric ion and PA detection
Using.
For this purpose, the technical solution adopted in the present invention is as follows:
(1) Ratiometric fluorescent probe is prepared；
(2) certain density PA standard solution is configured；
(3) ferric iron is added into fluorescence probe solution, makes probe gradually fluorescent quenching, obtain probe/trivalent ferrous solution；
(4) PA is added in the solution of fluorescent quenching in step (3), restores fluorescence probe；
(5) fluorescence intensity for measuring middle probe/ferric iron and PA reaction front and back, becomes according to PA concentration and relative intensity of fluorescence
Relationship between change value establishes standard curve；
(6) quantitative detection: the fluorescence intensity change before and after measurement sample to be tested and probe/ferric iron solution reaction calculates
Relative intensity of fluorescence changing value, the middle standard curve obtained of comparison step (5), obtains the content of PA in sample to be tested.
In the above method, the preparation method of Ratiometric fluorescent probe includes:
S1 embeds the preparation of the nano SiO 2 particle of red quantum dot:
Triton x-100 and n-hexyl alcohol are added sequentially in hexamethylene, red quantum dot is added after being uniformly mixed, in 5
~40 DEG C stirring 5~30min minutes, formed microemulsion system；Then in the microemulsion system be added ethyl orthosilicate and
Ammonium hydroxide, room temperature reaction for 24 hours, are demulsified to the isopropanol that 36ml is added in the reaction system after reaction, obtain flocculent deposit
Object is washed flocky precipitate centrifugation, alcohol repeatedly, until the centrifuged supernatant unstressed configuration signal of last time, after freeze-drying
Obtain embedding the nano SiO 2 particle of red quantum dot,
Wherein, the quality dosage of the triton x-100 is calculated as 3.5~4.0g/1ml with the volume of the red quantum dot；
The volumetric usage of the n-hexyl alcohol is calculated as 4~5 μ l/1 μ l with the volume of red quantum dot；The volumetric usage of the hexamethylene is with red amount
Son point volume is calculated as 15~20 μ l/1 μ l；The volumetric usage of the ethyl orthosilicate is calculated as 0.1~0.2 with the volume of red quantum dot
μl/1μl；The quality dosage of the ammonium hydroxide is calculated as 4~5g/1ml with the volume of red quantum dot；The volumetric usage of the isopropanol with
The volume of the reaction system is calculated as 3~4ml/1ml；
S2 packs the nano SiO 2 particle of the red quantum dot of embedding of standby amination modification:
The nano SiO 2 particle of the red quantum dot of embedding is dispersed in dehydrated alcohol, it is fast to the reaction solution
3- aminopropyl triethoxysilane is added in speed, uses serpentine condenser oil bath heating to 85~95 DEG C of reflux；After the reaction was completed, make
Its cooled to room temperature is centrifugated the product, is scattered in ultrapure water later, and the embedding for obtaining amination modification is red
The nano SiO 2 particle dispersion liquid of quantum dot,
Wherein, the quality dosage of the dehydrated alcohol is calculated as 8 with the nano SiO 2 particle quality for embedding red quantum dot
~12ml/1mg；The 3- aminopropyl triethoxysilane volumetric usage is to embed the nano SiO 2 particle matter of red quantum dot
Amount is calculated as 50~75 μ l/1mg,
S3, the preparation of blue quantum dot solution:
Lysine and citric acid are dissolved in ultrapure water, solid is transferred in ptfe autoclave after all dissolving
4~6h is reacted at 150~250 DEG C, and the solution in reaction kettle is dialysed 20~50h in ultrapure water using bag filter later,
Blue quantum dot after dialysis is stored in 4 DEG C of refrigerators,
Wherein, the amount of the substance of the lysine and citric acid is respectively 4~6mmol and 9~11mmo；The bag filter
Molecular cut off be 500Da.
S4, the preparation of Ratiometric fluorescent probe:
It weighs 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride and n-hydroxysuccinimide dissolves respectively
It in ultrapure water, is mixed later with the blue quantum dot solution of step S3 preparation, 27~33min of reaction stirring under dark condition；With
The nano SiO 2 particle dispersion of the red quantum dot of embedding of amination modification prepared by step 2 is added in backward above-mentioned reaction solution
Liquid, and 12h is reacted at room temperature；After reaction, it is centrifugated the product, obtains the Ratiometric fluorescent probe,
Wherein, 1- (3- the dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride quality dosage is with the blue quantum
The volume of point solution is calculated as 30mg/1ml；The n-hydroxysuccinimide quality dosage is with the volume of the blue quantum dot solution
It is calculated as 70mg/1ml.
Relative intensity of fluorescence changing value is according to formula (F-FA)/FA× 100% calculates, wherein FAExpression probe/ferric
Fluorescence intensity, F indicate probe/ferric iron reacted with various concentration PA after fluorescence intensity.
In step (3), the specific steps are the 0.5~1ml Ratiometric fluorescent probe solution for taking multiple groups same volume respectively
In quartz colorimetric utensil, it is separately added into the different iron chloride of 10 μ L concentration, standing 15min at room temperature reacts it completely.
In step (5), the specific steps are take respectively the 0.7ml Ratiometric fluorescent probe solution of multiple groups same volume in stone
In English cuvette, the ferric chloride solution that 10 μ L concentration are 10mM is added, standing 15min reacts it completely, is separately added into later
The phytic acid aqueous solution of 10 μ L various concentrations.
Compared with the prior art, the advantages of the present invention are as follows:
1, compared to other Ratiometric fluorescent probes, this probe when detecting, has quite outstanding specificity.And it is steady
The advantages that qualitative good, preparation is simple originally, cheap, and the reaction time is substantially reduced, it is low to the detection of ferric ion
To 0.83 μM.By comparison it can be found that this probe is more wider than the detection range of single blue quantum dot fluorescence probe, detection limit
It is lower.
2, the design of this research uses fluorescence " off-on " mode, due to Fe3+Fluorescent quenching effect for blue quantum dot
And phytic acid and Fe3+Between strong interaction, realize the Ratiometric fluorescent probe for the specific detection of phytic acid.It is logical
It crosses 3 δ/k formula and calculates its available detection limit (1.5 μM).With existing phytic acid detection method (ion mass spectrometry, efficient liquid
Phase chromatography, electrochemical process etc.) it compares, detection limit is comparable.
In view of the above problems, we devise a kind of Ratiometric fluorescent probe microballoon, it is capable of the identification ferric iron of selectivity
Ion, and pass through the new method of fluorescence Restoration Mechanism detection phytic acid.The technology quantum dot has fluorescence intensity high, toxicity is low,
The advantages that stability is good, and biocompatibility is preferable after modification, and light resistance is good, and fluorescence lifetime is long and bleach-resistant ability is strong, together
When this method it is easy to operate, detection quickly and high sensitivity.In addition, actually detected answer is subject to actual water sample using the technology
With working well.
Detailed description of the invention
Fig. 1 is the flow chart of preparation method of the present invention；
Fig. 2 is the TEM figure of blue quantum dot in the embodiment of the present invention 1；
Fig. 3 is the silica of the surface amination modification of the embedded quantum dots of red emission in the embodiment of the present invention 1
The TEM of nanosphere schemes；
Fig. 4 is the blue quantum dot prepared in the embodiment of the present invention 1, and the surface of the embedded quantum dots of red emission is according to plan
The silicon dioxide nanosphere of modification and the fluorescence spectra of Ratiometric fluorescent probe；
Fig. 5 is the blue quantum dot prepared in the embodiment of the present invention 1, and the surface of the embedded quantum dots of red emission is according to plan
The silicon dioxide nanosphere of modification and the infrared spectrogram of Ratiometric fluorescent probe；
Fig. 6 is Ratiometric fluorescent probe made from the embodiment of the present invention 2 under the ferric ion concentration of various concentration
Fluorescence emission spectrum and relative intensity of fluorescence ratio F0/FAWith the relationship of ferric ion concentration；
Fig. 7 is fluorescence emission of the Ratiometric fluorescent probe under various concentration phytic acid made from the embodiment of the present invention 3
Spectrum and relative fluorescence index of recovery (F0-FA)/FAWith the relationship of phytic acid concentration.
Specific embodiment
The present invention is described further combined with specific embodiments below, but protection scope of the present invention is not limited in
This.
Embodiment 1:
Referring to Fig. 1, a kind of preparation method of Ratiometric fluorescent probe, comprising the following steps:
The preparation of S1, blue quantum dot:
The present embodiment prepares the blue quantum dot of surface carboxyl groups modification using high temperature thermal decomposition method.Specific steps are as follows: by 0.73g
Lysine and 2.1g citric acid are dissolved in 20ml ultrapure water, and solid is transferred to after all dissolving equipped with 50mL polytetrafluoroethylene (PTFE)
In reaction kettle, 5h is reacted at 200 DEG C, later by reaction kettle cooled to room temperature.For remove reaction product in impurity and
Small molecule is dialysed the solution 36h using the bag filter that molecular weight is 500Da in ultrapure water, obtains surface carboxyl groups modification
The indigo plant quantum dot solution is stored in 4 DEG C of refrigerators by blue quantum dot solution, spare.Its TEM figure is shown in Fig. 2.As seen from the figure, it prepares
Blue quantum dot present it is spherical, dispersibility preferably.
S2, the red quantum dot of embedding nano SiO 2 particle preparation:
7.7ml hexamethylene, 1.42g triton x-100 and 1.8ml n-hexyl alcohol are sequentially added in 100ml round-bottomed flask, are mixed
The 400 red quantum dot solutions of μ l are added after closing uniformly, system magnetic agitation 10min is formed into stable reverse microemulsion liquid
System.Then to 50 μ L ethyl orthosilicates and 200 μ L ammonium hydroxide are added in flask, room temperature reaction is for 24 hours.After reaction to the reactant
The isopropanol that 36ml is added in system is demulsified, and obtains flocky precipitate.Reactant centrifugation, alcohol are washed repeatedly, until last
Until secondary centrifuged supernatant unstressed configuration signal, the nano SiO 2 particle product for embedding red quantum dot is obtained.By the product
Freeze-drying is used for subsequent reactions.
Embed the amination modification of the nano SiO 2 particle of red quantum dot: by lyophilized products made from 4mg step S2
It is dispersed in 40mL dehydrated alcohol, rapidly joins 250 μ L 3- aminopropyl triethoxysilanes to the reaction solution, magnetic force stirs
It mixes and is heated to 90 DEG C of reflux, reaction is for 24 hours.The upper end connection of condenser pipe is equipped with the drying tube of calcium chloride, prevents from containing in air
Vapor enters the reaction system.After the reaction was completed, make its cooled to room temperature, be centrifugated the product, later by its point
It dissipates in 4ml ultrapure water, obtains the nano SiO 2 particle dispersion liquid of the red quantum dot of embedding of amination modification.Its TEM figure
See Fig. 3.As seen from Figure 3, the nano particle is spherical in shape, and even particle distribution, particle size is between 30-40nm.By
TEM map can determine that red quantum dot is successfully embedded into silica interior.
The preparation of S3, Ratiometric fluorescent probe:
Weigh 180mg 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride and 420mg N- hydroxysuccinimidyl acyl
Imines is dissolved separately in 1ml ultrapure water, is mixed later with the blue quantum dot solution of 6ml step S1 preparation, anti-under dark condition
30min should be stirred.Then to the dioxy of the red quantum dot of embedding for the amination modification that the aforementioned preparation of 1ml is added in above-mentioned reaction solution
SiClx nanoparticulate dispersion, and 12h is reacted at room temperature.After reaction, it is centrifugated the product, by the production after separation
Object obtains the Ratiometric fluorescent probe.
It disperses above-mentioned Ratiometric fluorescent probe in 8ml deionized water, obtains Ratiometric fluorescent probe aqueous solution, and store up
There are 4 DEG C of refrigerators are spare.Its infrared spectrogram is shown in Fig. 4.Detection blank silica dioxide granule and the two of the red quantum dot of embedding respectively
The infrared spectroscopy of silica nano particle, the similarities and differences using blank nano SiO 2 particle as reference, in both comparisons structure.
In addition, the infrared spectrum for embedding the nano SiO 2 particle of red quantum dot occurs compared to blank Nano particles of silicon dioxide
Multiple and different absorption peak, 1675cm-1、950cm-1And 2980cm-1Respectively indicate be C=O stretching vibration absworption peak,
The out-of-plane bending vibration absorption peak of O-H and the stretching vibration absworption peak of C-H.To sum up, FTIR map further proves to have succeeded
Synthesis is embedded to the nano SiO 2 particle of red quantum dot.
Its fluorescence spectrum is shown in Fig. 5, as seen from Figure 5, the nano-probe under the excitation wavelength of 380nm, in 445nm and
There is obviously fluorescence peak at 633nm, they respectively represent the fluorescence emission peak of blue quantum dot and red quantum dot.
Embodiment 2: the detection of ferric ion concentration
Take 0.7ml embodiment 1 prepare Ratiometric fluorescent probe solution in the quartz colorimetric utensil of 1cm × 1cm, respectively plus
Enter the different ferric ion of 10 μ L concentration, standing 15min at room temperature reacts it completely.Using 380nm as excitation wavelength, 400-
750nm is scanning range, its fluorescence emission spectrum is measured under conditions of slit width is 2.5nm.As a result see Fig. 6.
By Fig. 5 it can be found that Ratiometric fluorescent probe is in 445nm as the ferric ion concentration of addition constantly increases
The fluorescence intensity at place constantly reduces, and its fluorescence intensity at 633nm does not change substantially.Simultaneously it can also be seen that ratio
Fluorescence intensity ratio (the F of type fluorescence probe0/FA) and ferric ion concentration good linear relationship is showed between 9-120 μM
(R2=0.995), detection is limited to 0.26 μM.
It takes one group of Ratiometric fluorescent probe solution in quartz colorimetric utensil, the ferric ion solution of unknown concentration is added,
Standing 10min at room temperature reacts it completely.Using 380nm as excitation wavelength, 400-750nm is scanning range, in slit width
It is to measure its fluorescence emission spectrum under conditions of 2.5nm.Calculating ferric ion concentration according to linear relationship is 18.8 μM,
The result is identical as ICP testing result.
Embodiment 3: the detection of phytic acid concentration
The Ratiometric fluorescent probe aqueous solution for taking 0.7ml embodiment 1 to prepare is in the quartz colorimetric utensil of 1cm × 1cm, respectively
The ferric chloride solution that 10 μ L concentration are 10mM is added, standing 15min at room temperature reacts it completely, is separately added into 10 μ L again later
The phytic acid aqueous solution of various concentration.Using 380nm as excitation wavelength, 400-750nm is scanning range, is in slit width
The fluorescence emission spectrum of the Ratiometric fluorescent probe is measured under conditions of 2.5nm.As a result see Fig. 7.
As seen from Figure 6, being continuously added with PA, the fluorescence of Ratiometric fluorescent probe blue quantum dot at 445nm
It is gradually recovered, and fluorescence intensity of the red quantum dot at 633nm does not have significant change.The fluorescence of the Ratiometric fluorescent probe restores
Index (F0-FA)/FAGood linear relationship (R is showed between 0.08-1.6mM with the concentration of PA2=0.990).Wherein, F0
And FARespectively indicate fluorescence intensity of the Ratiometric fluorescent probe at 445nm and 633nm.
It takes one group of Ratiometric fluorescent probe solution in quartz colorimetric utensil, it is molten that the iron chloride that 10 μ L concentration are 10mM is added
Liquid, standing 15min at room temperature reacts it completely.The plant acid solution for adding unknown concentration, using 380nm as excitation wavelength,
400-750nm is scanning range, its fluorescence emission spectrum is measured under conditions of slit width is 2.5nm.It is closed according to linear
It is 0.94mM that system, which calculates phytic acid concentration, and the result is identical as high performance liquid chromatography testing result.
Embodiment 4: the selectivity of ratio fluorescent probe
In order to test the specificity that the Ratiometric fluorescent probe detects ferric ion, we have chosen some common
Metal ion is measured.It is separately added into 10mM interfering substance in Ratiometric fluorescent probe aqueous solution prepared by embodiment 1, such as
K+,Fe2+,Na+,Mg2+,Ca2+,Zn2+,Cu2+,Ni2+,Cr3+,Pb2+,Al3+,Ba2+, with Fe3+It is carried out under identical testing conditions
Fluoremetry.The experimental results showed that removing Fe3+Except other interfering substances all Ratiometric fluorescent probe fluorescence will not be caused strong
The variation for spending ratio, illustrates that Ratiometric fluorescent probe can be to ferric ion Selective recognition.
In order to determine specificity that Ratiometric fluorescent probe detects phytic acid, we have chosen some Common materials (metals
Cation, anion, bovine serum albumin) carry out interference measurement.10mM Fe is added into probe dispersion liquid3+, 15min is stood,
After it is stablized, 10mM interfering substance is added into the Ratiometric fluorescent probe-ferric ion compound system dispersion liquid, such as
BSA,K+,Na+,Mg2+,Zn2+,Cu2+,Fe2+,Cl-,SO4 2-,NO3-Deng being carried out in situation identical with the testing conditions of phytic acid
Fluoremetry.The experimental results showed that the other interfering substances removed except phytic acid cannot cause the fluorescence of the probe to restore, say
Bright Ratiometric fluorescent probe can be to phytic acid Selective recognition.
Claims (10)
1. a kind of preparation method of Ratiometric fluorescent probe, it is characterised in that the following steps are included:
S1 embeds the preparation of the nano SiO 2 particle of red quantum dot:
Triton x-100 and n-hexyl alcohol are added sequentially in hexamethylene, red quantum dot is added after being uniformly mixed, in 5~40
DEG C stirring 5~30min minute, formation microemulsion system；Then to addition ethyl orthosilicate and 25% in the microemulsion system
~28% ammonia spirit, room temperature reaction for 24 hours, are demulsified to the isopropanol that 36ml is added in the reaction system after reaction,
Flocky precipitate is obtained, flocky precipitate centrifugation, alcohol are washed repeatedly, until the centrifuged supernatant unstressed configuration signal of last time
Until, the nano SiO 2 particle for embedding red quantum dot is obtained after freeze-drying；
Wherein, the quality dosage of the triton x-100 is calculated as 3.5~4.0g/1ml with the volume of the red quantum dot；It is described
The volumetric usage of n-hexyl alcohol is calculated as 4~5 μ l/1 μ l with the volume of red quantum dot；The volumetric usage of the hexamethylene is with red quantum dot
Volume is calculated as 15~20 μ l/1 μ l；The volumetric usage of the ethyl orthosilicate is calculated as 0.1~0.2 μ l/1 with the volume of red quantum dot
μl；The quality dosage of the ammonium hydroxide is calculated as 4~5g/1ml with the volume of red quantum dot；The volumetric usage of the isopropanol is with described
The volume of reaction system is calculated as 3~4ml/1ml；
S2, the nano SiO 2 particle of the red quantum dot of embedding of preparation amination modification:
The nano SiO 2 particle of the red quantum dot of embedding is dispersed in dehydrated alcohol, is quickly added to the reaction solution
Enter 3- aminopropyl triethoxysilane, uses serpentine condenser oil bath heating to 85~95 DEG C of reflux；After the reaction was completed, make it certainly
It is so cooled to room temperature, is centrifugated the product, is scattered in ultrapure water later, obtain the red quantum of embedding of amination modification
The nano SiO 2 particle dispersion liquid of point；
Wherein, the quality dosage of the dehydrated alcohol to embed the nano SiO 2 particle quality of red quantum dot be calculated as 8~
12ml/1mg；The volumetric usage of the 3- aminopropyl triethoxysilane is to embed the nano SiO 2 particle matter of red quantum dot
Amount is calculated as 50~75 μ l/1mg；
S3, the preparation of blue quantum dot solution:
Lysine and citric acid are dissolved in ultrapure water, solid all dissolution after be transferred in ptfe autoclave in
4~6h is reacted at 150~250 DEG C, the solution in reaction kettle is dialysed 20~50h in ultrapure water using bag filter later, thoroughly
The blue quantum dot obtained after analysis is stored in 4 DEG C of refrigerators；
Wherein, the amount of the substance of the lysine and citric acid is respectively 4~6mmol and 9~11mmo；The bag filter is cut
Staying molecular weight is 500Da；Dosage in the ultrapure water is enough all to dissolve the lysine and citric acid；
S4, the preparation of Ratiometric fluorescent probe:
Weigh 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride and n-hydroxysuccinimide be dissolved separately in it is super
It in pure water, is mixed later with the blue quantum dot solution of step S3 preparation, 27~33min of reaction stirring under dark condition；Then to
The nano SiO 2 particle dispersion liquid of the red quantum dot of embedding of the amination modification of step S2 preparation is added in above-mentioned reaction solution,
And 12h is reacted at room temperature；After reaction, it is centrifugated the product, obtains the Ratiometric fluorescent probe；
Wherein, the quality dosage of 1- (3- the dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride is with the blue quantum dot
The volume of solution is calculated as 30mg/1ml；The quality dosage of the n-hydroxysuccinimide is with the volume of the blue quantum dot solution
It is calculated as 70mg/1ml.
2. the preparation method of Ratiometric fluorescent probe according to claim 1, it is characterised in that: the red quantum dot is selenium
Cadmium, the indigo plant quantum dot is carbon dots.
3. the preparation method of Ratiometric fluorescent probe according to claim 1, it is characterised in that: in step S1, in 20 DEG C
Stirring 5~30min minutes.
4. the preparation method of Ratiometric fluorescent probe according to claim 1, it is characterised in that: in step S2, use snake
Shape condenser pipe oil bath heating to 90 DEG C flow back.
5. the preparation method of Ratiometric fluorescent probe according to claim 1, it is characterised in that: in step S3, solid is complete
It is transferred in ptfe autoclave at 200 DEG C after portion's dissolution and reacts 5h, it later will be molten in reaction kettle using bag filter
Liquid is dialysed 36h in ultrapure water；The amount of the substance of the lysine and citric acid is respectively 5mmol and 10mmol.
6. the preparation method of Ratiometric fluorescent probe according to claim 1, it is characterised in that: in step S4, in dark
Under the conditions of reaction stirring 30min.
7. the preparation method of Ratiometric fluorescent probe according to claim 1, it is characterised in that: the amount of indigo plant described in step S4
The volume of the silica nanometer dispersion liquid for the red quantum dot of embedding that the volumetric usage of son point is modified with the amination is calculated as
6ml/1ml。
8. Ratiometric fluorescent probe prepared by the method for any one of claim 1-7 a kind of answering in detection ferric ion
With, it is characterised in that the following steps are included:
1) it takes the Ratiometric fluorescent probe of multiple groups same volume to be respectively placed in quartz colorimetric utensil, it is different to be separately added into 10 μ L concentration
Ferric chloride solution, at room temperature stand 15min react it completely；
2) using 380nm as excitation wavelength, 400-750nm is scanning range, and step is measured under conditions of slit width is 2.5nm
It is rapid 1) in each sample fluorescence emission spectrum, it is dense with the fluorescence intensity ratio (F0/FA) and ferric ion of Ratiometric fluorescent probe
Degree is that coordinate draws standard curve, wherein F0 and FA is respectively that fluorescence of the Ratiometric fluorescent probe at 445nm and 633nm is strong
Degree；
3) fluorescence intensity change of 50 μ L samples to be tested and Ratiometric fluorescent probe reaction front and back, meter are measured using Fluorescence Spectrometer
The fluorescence intensity ratio for calculating Ratiometric fluorescent probe, the standard curve obtained with step 2) compare, and obtain ferric iron in sample to be tested
The content of ion.
Above-mentioned steps 1), 3) in, the volume of Ratiometric fluorescent probe is 0.5~1ml.
9. the application of Ratiometric fluorescent probe prepared by the method for any one of claim 1-7 a kind of in detection phytic acid,
Be characterized in that the following steps are included:
1) Ratiometric fluorescent probe of multiple groups same volume is taken to be respectively placed in quartz colorimetric utensil, being separately added into 10 μ L concentration is
The ferric chloride solution of 10mM, standing 15min reacts it completely；
2) the phytic acid aqueous solution of 10 μ L various concentrations is separately added into Xiang Shangshu quartz colorimetric utensil, using 380nm as excitation wavelength,
400-750nm is scanning range, and the fluorescent emission of the Ratiometric fluorescent probe is measured under conditions of slit width is 2.5nm
Spectrum, with the fluorescence index of recovery (F of Ratiometric fluorescent probe0-FA)/FAConcentration with phytic acid aqueous solution is that coordinate makes standard
Curve, wherein F0And FARespectively fluorescence intensity of the Ratiometric fluorescent probe at 445nm and 633nm.
3) ferric chloride solution that 10 μ L concentration are 10mM is added in Xiang Suoshu Ratiometric fluorescent probe, being stored at room temperature 15min makes it
Reaction completely, is then added 50 μ L samples to be tested, measures sample to be tested using Fluorescence Spectrometer and fluorescence probe reacts front and back
Fluorescence intensity change, calculates relative intensity of fluorescence changing value, and the standard curve that control step 2) obtains obtains PA in sample to be tested
Content.
Above-mentioned steps 1), 3) in, the volume of Ratiometric fluorescent probe is 0.5~1ml.
10. Ratiometric fluorescent probe prepared by the method for any one of claim 1-7 a kind of.
The partial size of the red quantum dot of nano SiO 2 particle of the red quantum dot of embedding of amination modification is 20~50nm, preferably 30
~40nm.
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| CN110308031A (en) * | 2019-07-24 | 2019-10-08 | 广州翰德泽信医药科技有限公司 | A kind of stable fungi fluorescent staining liquid |
| CN111504961A (en) * | 2020-03-31 | 2020-08-07 | 南昌大学 | Fluorescent sensor based on glutathione gold nanoclusters and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104777156A (en) * | 2015-04-04 | 2015-07-15 | 天津大学 | Method for detecting phytic acid based on carbon dot fluorescence off-on mode |
| CN105911030A (en) * | 2016-04-05 | 2016-08-31 | 中国科学院合肥物质科学研究院 | Ratio fluorescence sensor and visualized detection method for glucose |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104777156A (en) * | 2015-04-04 | 2015-07-15 | 天津大学 | Method for detecting phytic acid based on carbon dot fluorescence off-on mode |
| CN105911030A (en) * | 2016-04-05 | 2016-08-31 | 中国科学院合肥物质科学研究院 | Ratio fluorescence sensor and visualized detection method for glucose |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110308031A (en) * | 2019-07-24 | 2019-10-08 | 广州翰德泽信医药科技有限公司 | A kind of stable fungi fluorescent staining liquid |
| CN111504961A (en) * | 2020-03-31 | 2020-08-07 | 南昌大学 | Fluorescent sensor based on glutathione gold nanoclusters and application thereof |
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