CN109998015A - The purposes of the sub- fruit of root of Narrowfruit Currant or its extract - Google Patents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
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Abstract
The present invention provides the sub- fruits of root of Narrowfruit Currant or its extract to prepare the purposes in antimicrobial product.The research of the invention finds that the sub- fruit of root of Narrowfruit Currant or its extract have good antibacterial activity, it is more prominent especially for the inhibitory activity of Candida albicans and pseudomonas aeruginosa.
Description
Technical field
The invention belongs to the purposes of a kind of sub- fruit of root of Narrowfruit Currant or its extract.
Technical background
Root of Narrowfruit Currant (Ribes stenocarpum Maxim.) belongs to Saxifragaceae currant platymiscium, fills for fallen leaves
Wood is born under 2800 meters of height above sea level hillside shrubberies below, shaw or in gully.Currant platymiscium morphological variation is larger, class
Type is complicated, and the whole world is distributed mainly on Europe and the warm area of North American climate, also divides in Asia, South America and north African there are about 160 kinds
Cloth is extensive, there is 59 kinds of 30 mutation within Chinese territory, and only 11 kinds of 1 mutation are just distributed in Qinghai Province." Goats in Liangshan Prefecture Chinese herbal medicine resource is general
Look into register " record the stem of root of Narrowfruit Currant and branch can be used for the treatment of hepatitis, recorded in " Jingzhubencao " currant hold back it is malicious, remove
Yellow water can simultaneously restrain the effect of various vascular disorders.
The fruit of root of Narrowfruit Currant is succulence berry, and unique flavor multiplicity, from bitter, acid to sweet and sour taste, common people are usual
The fruit of currant is eaten raw and is used for anti-cure cold.
Summary of the invention
It is an object of the invention to the further research and development to the sub- fruit of root of Narrowfruit Currant.
Specifically, the present invention provides the sub- fruits of root of Narrowfruit Currant or its extract to prepare the purposes in antimicrobial product.
Further, the extract is dissolvable in water in the water of pH1~3.
In the sub- fruit extract of root of Narrowfruit Currant, in terms of dry product, organic acid content is 3.5~5.0%w/w, flavones
Content is in 0.5%w/w or less.
" w/w " of the present invention is mass ratio, can be g/g, is also possible to kg/kg, mg/mg etc..
In a specific embodiment of the invention, organic acid content is 4.0~4.5% in provided extract, flavones
Content is 0%.
Said extracted object is practical be the sub- fruit of root of Narrowfruit Currant ethanol extract.Further, the preparation of the extract
Method includes following content:
(1) the sub- fruit ethanol extract of root of Narrowfruit Currant after removing ethyl alcohol；
(2) water phase adjusts pH to 10~12, is extracted with ethyl acetate colourless or close to colourless to water phase；
(3) water phase adjusts pH to 1~3, is extracted with ethyl acetate colourless or close to colourless to water phase；
(4) merge water phase to get the sub- fruit extract of root of Narrowfruit Currant.
Dried extract or aqueous extract can be prepared according to demand.Solvent can be removed.
The research of the invention finds that above-mentioned water-soluble extractive has good antibacterial activity, especially for Candida albicans
It is more prominent with the inhibitory activity of pseudomonas aeruginosa.
Wherein, in the preparation process of extract, the concentration of alcohol is 60~85%v/v.
In a specific embodiment of the invention, concentration of alcohol is selected from 70~80%v/v, can be 70%, 71%,
72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%.
Wherein, pH is adjusted using alkali in content (2), is water-soluble alkali, uses inorganic base, such as sodium hydroxide, hydrogen-oxygen more
Change potassium.
Wherein, acid for adjusting pH is used in content (3), uses inorganic acid, such as hydrochloric acid, hydrobromic acid more.
In the present invention, the preparation method of ethanol extract is mentioned using conventional method, such as heating extraction, room temperature
It takes, the extraction of pressurised extraction, normal pressure, specific manifestation can be refluxing extraction, Soakage extraction, seep clear extraction, microwave radiation exaraction, surpass
Sound extraction, homogenate extraction etc..
In the present invention, ethyl acetate is extracted, and mainly extracts separate substance from water phase using ethyl acetate, phase is utilized
Different ingredients is separated by the insoluble property of dissolubility difference, ethyl acetate and water like compatibility principle.
" ethyl alcohol is removed " in the present invention to refer to, as far as possible removes the ethyl alcohol in extracting solution.Certainly, in behaviour commonly used in the art
Make in method, ethyl alcohol is often difficult to go to the greatest extent, it is possible that having the case where ethyl alcohol residual, but a small amount of ethyl alcohol residual also belongs to this
The protection category of invention.Common method is mostly to be recovered under reduced pressure, naturally it is also possible to normal heating volatilization ethyl alcohol.
" solvent is removed " in the present invention, it is similar to " removing ethyl alcohol ".
In the specific embodiment of the invention, the preparation of the sub- fruit ethanol extract of root of Narrowfruit Currant uses dry fruit, but this
It is not intended to limit and is extracted using fresh fruit.
In the present invention, the bacterium includes but is not limited to Escherichia coli, salmonella, staphylococcus aureus, withered grass bud
One of spore bacillus, Candida albicans, verdigris bacillus are a variety of.
The research of the invention finds that the extract of preceding method preparation, has inhibitory activity to above-mentioned bacterium, but read white
Pearl bacterium, the inhibitory activity of verdigris bacillus are especially prominent.
Heretofore described " antimicrobial product ", as antibacterial agent are including but not limited to used in drug, additive, preservative
Etc. various aspects.In actual production, using the requirement that should meet related administrative regulation.
Specific embodiment
A variety of materials information used in the specific embodiment of the invention is as follows.
The sub- fruit of root of Narrowfruit Currant picks up from Huzhu County, Qinghai Province in September, 2017, by Agriculture and Animal Husbandry College, Qinghai University grandson Hai Qunjiao
It awards and is accredited as sub (the Ribes stenocarpum Maxim.) fruit of root of Narrowfruit Currant, drying and crushing is spare.Escherichia coli
(Escherichia coli), salmonella (Salmonella), staphylococcus aureus (Staphylococcus
Aureus), bacillus subtilis (Bacillus subtilis), Candida albicans (Monilia albican), verdigris gemma
Bacillus (Pseudomonas aeruginosa) is purchased from Hangzhou Basebio Bio-tech Co., Ltd..
Experimental animal: healthy kunming mice 50, weight (20 ± 2g), SPF grades, by Chinese Academy of Agricultural Sciences Lanzhou animal doctor
Research institute provides.
Same and development in science and technology Co., Ltd is started in KC-130 micromill Beijing；DF-101S heat collecting type heated at constant temperature magnetic
Power blender Henan Yu Hua Instrument Ltd.；HH-4 digital display thermostat water bath Guo Hua Electrical Appliances Co., Ltd；RE-52A rotation is steamed
Fa Yi Shanghai Yarong Biochemical Instrument Plant；250B biochemical cultivation case Changzhou Guohua Electric Appliance Co., Ltd.；YM-75 vertical pressure steam goes out
Jun Qi Shenan Medical Appliances Factory, Shanghai；UV-2600 ultraviolet-uisible spectrophotometer Shimadzu business administration Co., Ltd；SC2201
Refiner Shanghai little Yan industry and trade Development Co., Ltd；XW-80A swirl mixing device Shanghai Chi Tang Electronics Co., Ltd.；H/T16MM-
The conspicuous Western-style clothes in table model high speed centrifuge Hunan is for Instrument Ltd.；SM600 microplate reader Shanghai Yongchuang Medical Instrument Co., Ltd.；
DHG-9240A electric drying oven with forced convection Shanghai Yiheng Scientific Instruments Co., Ltd；14-0807 supersonic wave cleaning machine Ningbo Xin Zhisheng
Object Science and Technology Co., Ltd.；ESJ110-4B electronic balance Shenyang Longteng Electronic Co., Ltd..
The biological reagents such as MH agar medium, MH broth bouillon are purchased from Hangzhou Basebio Bio-tech Co., Ltd.；Inose
Former (MG) kit, hepatic glycogen (LG) kit, serum urea nitrogen (BUN) kit, lactic dehydrogenase (LDH) kit are purchased
Bioengineering Research Institute, dehydrated alcohol, ethyl acetate, n-butanol, petroleum ether, sodium hydroxide, sodium nitrite, nitre are built up from Nanjing
Sour aluminium etc. is that analysis is pure.
The preparation (i.e. organic acids extract I) of the extract of the present invention of embodiment 1
The sub- fruit sample of 100g root of Narrowfruit Currant is weighed, 75% ethyl alcohol is added and impregnates 1h, is heated to reflux in Soxhlet extractor
It extracts twice, each 2-3h is filtered after cooling, collects filtrate and 5% sodium hydroxide solution tune PH is added after being concentrated into no alcohol taste
To 11, with ethyl acetate, extraction solution collects buck layer to colourless repeatedly, 5% hydrochloric acid tune PH to 2, then repeatedly with ethyl acetate
It is extracted to colourless, solvent is recovered under reduced pressure in combining extraction liquid, obtains organic acids extract I.
The preparation (i.e. organic acids extract I) of the extract of the present invention of embodiment 2
The sub- fruit sample of 1000g root of Narrowfruit Currant is weighed, 70% ethyl alcohol is added and impregnates 2h, the Soakage extraction 3 at 40~50 DEG C
It is secondary, it is 4-12 hours each, it collects extracting solution and 10% potassium hydroxide solution tune PH to 12 is added after being concentrated into no alcohol taste, use acetic acid
Extraction solution collects buck layer, 5% hydrobromic acid tune PH to 3 to colourless to ethyl ester repeatedly, then is extracted to nothing repeatedly with ethyl acetate
Solvent is recovered under reduced pressure in color, combining extraction liquid, obtains organic acids extract I.
In follow-up test of the present invention, extract is prepared into dry extract, convenient for calculating.
Total organic acids assay weighs the sub- fruit difference extract 0.5g of root of Narrowfruit Currant respectively, and 50% ethyl alcohol dissolves and determines
Hold to 50mL, draws 10mL in 250mL conical flask, add 1% phenolphthalein indicator 2 to drip, with the sodium hydroxide standard of 0.1mol/L
Liquid is titrated to neutrality, records sodium hydroxide solution dosage, calculates total organic acids content according to following equation, is averaged in triplicate
Value.
Total organic acids content (%)=(C × V × 40 × K)/(m × 10/50) × 100% (formula 1)
In formula: V- titration consumption sodium hydroxide solution volume (mL)；C- NaOH titer titration concentration (mol/L)；M- sample
Quality (g)；K- is converted into main sour coefficient, and (i.e. 1 mM of sodium hydroxide is equivalent to the coefficient of main acid, and kernel approaches use
Tartaric acid, currant are drupe, K=0.075).
The measurement result of total organic acids content
The organic acid content testing result of the different extracts of table 1
As shown in Table 1, the content of total organic acids is variant in root of Narrowfruit Currant fruit opposed polarity position, and organic acid extracts
Organic acid content highest in object I is 4.18%, and the organic acid content of n-butanol extract IV is minimum, is 0.66%, two kinds of acetic acid
Organic acid content difference is little in ethyl ester extract, illustrates ethyl acetate extract II during macroporous adsorbent resin column chromatography
Organic acid primary attachment is in polyamide macroporous absorbent resin, and the later period can effectively recycle most of organic acid by buck elution,
Achieve the purpose that organic acid preliminary concentration purifies.
Determination of total flavonoids draws rutin standard curve: dissolving control substance of Rutin 10mg with 80% ethyl alcohol, is settled to
25ml, it is accurate draw reference substance 0.5,1.0,1.5,2.0,2.5,3.0,3.5,4.0,4.5mL be respectively placed in 10mL volumetric flask,
The absorbance value that each solution is measured using sodium nitrite-aluminum nitrate spectrophotometry is inhaled using rutin concentration of standard solution as abscissa
Shading value is ordinate, draws standard curve.
Determination of total flavonoids in sample: weighing the sub- fruit difference extract 0.1g of root of Narrowfruit Currant respectively, and 80% ethyl alcohol is molten
It is settled to 50mL after solution, draws 0.5mL sample in 10mL volumetric flask, 5% sodium nitrite solution 0.3mL is added and stands 6min,
10% aluminum nitrate solution 0.3mL is added and stands 6min, adds 4% sodium hydroxide solution 4mL, distilled water is fixed after sufficiently reacting
Hold, the light absorption value of sample solution is measured at 356nm, is contained according to the general flavone that calibration curve equation and following equation calculate sample
Amount.
General flavone content (%)=(C × K × V)/M × 100% (formula 2)
In formula: sample general flavone concentration (mg/mL) corresponding to the different absorbance values of C- measurement；K- extension rate；V-
Sample solution volume (mL) after pipetting dilution；The weighed sample quality (g) of M-.
Standard curve and the measurement of general flavone compounds content
Rutin standard curve and regression equation
Rutin standard curve regression equation: y=30.421x+0.1623, R2=0.9952, meet linear relationship.
General flavone compounds content measurement result
General flavone compounds content measurement result in the different extracts of table 2
As shown in Table 2, chromocor compound is primarily present in ethyl acetate extract II, ethyl acetate extract III and positive fourth
In alcohol extracting thing IV, and general flavone compounds content is closer in three kinds of extracts, illustrates to be distributed in the sub- fruit of root of Narrowfruit Currant
In chromocor compound polarity difference it is larger.
Other different extracts the preparation method is as follows:
The preparation of ethyl acetate extract and n-butanol extract weighs the sub- fruit sample of a certain amount of root of Narrowfruit Currant, and 90%
Ethyl alcohol heating and refluxing extraction three times, collects filtrate, and filter residue uses 70% ethyl alcohol to extract three times again, and filtering merges all filtrates, concentration
Petroleum ether extraction is used afterwards, discards petroleum ether layer, water phase is extracted with ethyl acetate, and organic phase is concentrated, obtains ethyl acetate extract II,
Water phase uses extracting n-butyl alcohol again, and n-butanol layer is concentrated, obtains n-butanol extract IV.Ethyl acetate extract II crosses polyamide macropore
Resin is adsorbed, successively with the distilled water of 2 times of column volumes, 30% ethyl alcohol, 50% ethyl alcohol, 70% ethyl alcohol and anhydrous ethanol elution, so
Resin is poured out afterwards, with 2% salt acid soak resin 2h, distillation is washed to neutrality, and it adds 2% sodium hydroxide solution and impregnates 2h,
Filtrate is collected by filtration, is concentrated to get ethyl acetate extract III.
The bacteriostatic activity of the sub- fruit extract of root of Narrowfruit Currant is studied
The preparation of 1 bacteria suspension is chosen Escherichia coli, salmonella, staphylococcus aureus, bacillus subtilis, white and is read
Pearl bacterium, verdigris bacillus are seeded to broth bouillon and prepare bacteria suspension, 37 DEG C of constant temperature trainings as tested strain after slant activation
4h is supported, sterile saline dilutes culture solution, with Maxwell opacity tube than turbid to 0.5 maxwell unit.
The preparation of 2 susceptibility pieces weighs 5g extract, and the heating of 50% ethanol water bath makes to dissolve, and adjustment liquor strength is respectively
100mg/mL, 200mg/mL, 300mg/mL immerse sterilizing blank susceptibility piece in various concentration medical fluid, dry after immersion 12h standby
With.
The bacteriostasis that 3 K-B disk diffusion methods measure different extracts will melt and the agar medium to sterilize is cooled to
50 DEG C of inverted plates are spare, and cotton swab dips bacteria suspension even spread culture medium, are repeated several times, and plate is rotated 60 degree every time.Use tweezer
The susceptibility piece for being soaked with various concentration extract is affixed on media surface by son respectively, is flattened with tweezer point, was impregnated with 50% ethyl alcohol
Susceptibility piece as blank control, with ampicillin (10IU/ piece), gentamicin (10IU/ piece), penicillin (10IU/ piece) medicine
Quick is positive control, and for 24 hours, vernier caliper measurement colony diameter, right-angled intersection measurement is averaged 37 DEG C of constant temperature incubations.
4 doubling dilutions measure minimal inhibitory concentration and minimum bactericidal concentration prepares the sub- fruit of root of Narrowfruit Currant of 200mg/mL
Extract takes the different extracts of 1 volume and sterilized Liquid Culture to be based in test tube respectively, is uniformly mixed, to dilute again
Interpretation of the law adds isometric dilution grade bacterium solution to each antibacterial pipe, shakes up, keep different extracts final concentration of by pipe dilute liquid medicine
50,25,12.5,6.25,3.13,1.56mg/mL, and blank control is set, each test tube is placed in 37 DEG C of incubators and is cultivated
24h。
The judgement of minimum inhibitory concentration (MIC): the cloudiness of each test tube after observation culture, test tube clarify and after shaking up still
Defecator, it is believed that the pipe asepsis growth shows there is bacterium growth if being in cloudy state in test tube, finds out from asepsis growth test tube
Minimum concentration test tube, corresponding to liquor strength be minimum inhibitory concentration.
The judgement of minimum bactericidal concentration (MBC): each pipe for having no bacterial growth is successively pipetted into appropriate amount of fluid even spread
In in solid medium tablets, for 24 hours, minimum liquor strength of the clump count less than 5 is killed as minimum using on plate for 37 DEG C of cultures
Bacteria concentration (MBC).
The bacteriostatic activity result of 5 extracts
5.1 K-B disk diffusion method experimental results and analysis
Antibacterial circle diameter when 3 extract concentrations of table are 100mg/mL
Note: it is extremely quick that antibacterial circle diameter, which is greater than 20mm,；15-20mm is Gao Min；10-15mm be in it is quick；7-9mm is muting sensitive；
It is insensitive less than 6mm.
Inhibition zone size when 4 extract concentrations of table are 200mg/mL
Note: it is extremely quick that antibacterial circle diameter, which is greater than 20mm,；15-20mm is Gao Min；10-15mm be in it is quick；7-9mm is muting sensitive；
It is insensitive less than 6mm.
Inhibition zone size when 5 extract concentrations of table are 300mg/mL
Note: it is extremely quick that antibacterial circle diameter, which is greater than 20mm,；15-20mm is Gao Min；10-15mm be in it is quick；7-9mm is muting sensitive；
It is insensitive less than 6mm.
By table 3, table 4, table 5 it is found that different extracts are variant to the inhibitory activity of different bacterium.Organic acids extract I
It is also good to Candida albicans and pseudomonas aeruginosa inhibitory effect, it is better than positive control drug penicillin when concentration is 300mg/mL
(10IU/ piece), when concentration 100mg/mL, 200mg/mL respectively with ampicillin (10IU/ piece) and gentamicin (10IU/ piece)
Quite.
5.2 minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC)
The different extracts of table 6 are to Candida albicans MIC and MBC value measurement result
The different extracts of table 7 are to pseudomonas aeruginosa MIC and MBC value measurement result
By table 6, table 7 it is found that different extracts are variant to MIC the and MBC value of different strain in the sub- fruit of root of Narrowfruit Currant,
Wherein organic acids extract I and ethyl acetate extract II are best to Candida albicans and pseudomonas aeruginosa antibacterial activity, and
MIC value is 1.56mg/mL, and MBC value is 3.13mg/mL.
Claims (10)
1. the sub- fruit of root of Narrowfruit Currant or its extract are preparing the purposes in antimicrobial product.
2. purposes according to claim 1, it is characterised in that: the extract is dissolvable in water in the water of pH1~3.
3. purposes according to claim 2, it is characterised in that: in the extract, organic acid content is 3.5~5.0%
W/w, flavones content is in 0.5%w/w or less.
4. purposes according to claim 3, it is characterised in that: organic acid content is 4.0~4.5%, flavones content 0.
5. purposes according to any one of claims 1-4, it is characterised in that: the extract is the sub- fruit of root of Narrowfruit Currant
Ethanol extract.
6. purposes according to any one of claims 1-4, it is characterised in that: the preparation method of the extract includes such as
Lower content:
(1) the sub- fruit ethanol extract of root of Narrowfruit Currant after removing ethyl alcohol；
(2) water phase adjusts pH to 10~12, is extracted with ethyl acetate colourless or close to colourless to water phase；Further, pH is selected from
11；
(3) water phase adjusts pH to 1~3, is extracted with ethyl acetate colourless or close to colourless to water phase；Further, pH is selected from 2；
(4) merge water phase, the sub- fruit extract of root of Narrowfruit Currant is prepared.
7. purposes according to claim 5 or 6, it is characterised in that: the concentration of alcohol is 60~85%v/v；Further
Ground, the concentration of alcohol are 70~80%v/v.
8. purposes according to claim 4, it is characterised in that: adjust pH using inorganic base in content (2)；It is further selected from
One of sodium hydroxide, potassium hydroxide are a variety of；Inorganic acid for adjusting pH is used in content (3)；It is further selected from hydrochloric acid, hydrogen bromine
One of acid is a variety of.
9. purposes according to claim 1, it is characterised in that: the bacterium is selected from Escherichia coli, salmonella, golden yellow
One of staphylococcus, bacillus subtilis, Candida albicans, verdigris bacillus are a variety of；It is further selected from white thought
One of pearl bacterium, verdigris bacillus are a variety of.
10. a kind of antimicrobial product, it is characterised in that: it is active constituent that it, which is by the sub- fruit of root of Narrowfruit Currant or its extract,.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811509345.4A CN109998015A (en) | 2018-12-11 | 2018-12-11 | The purposes of the sub- fruit of root of Narrowfruit Currant or its extract |
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| CN201811509345.4A CN109998015A (en) | 2018-12-11 | 2018-12-11 | The purposes of the sub- fruit of root of Narrowfruit Currant or its extract |
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| CN109998015A true CN109998015A (en) | 2019-07-12 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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