CN109997838A - A kind of practical cell cryopreservation cooling box - Google Patents
A kind of practical cell cryopreservation cooling box Download PDFInfo
- Publication number
- CN109997838A CN109997838A CN201910237680.1A CN201910237680A CN109997838A CN 109997838 A CN109997838 A CN 109997838A CN 201910237680 A CN201910237680 A CN 201910237680A CN 109997838 A CN109997838 A CN 109997838A
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- CN
- China
- Prior art keywords
- main body
- cell cryopreservation
- cooling box
- body container
- container
- Prior art date
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
- A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
- A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
Abstract
The invention discloses a kind of practical cell cryopreservation cooling boxes, including main body container A, box cover, sealing-plug B, sealing vacuum layer and free storehouse, the main body container A is cylindrical structure, and the main body container A inner upper is arranged with box cover, and the box cover internal sleeve is equipped with sealing-plug B.The beneficial effects of the present invention are: extremely convenient and practical: conventional cell freezes it is generally necessary to 4 DEG C, -20 DEG C of refrigerators and -80 DEG C of refrigerators, this container is directly hung in liquid nitrogen container, the demand to three kinds of refrigerators is eliminated, the step of container is transferred to different temperatures environment is also eliminated.Capacity is big: under identical appearance volume, compared with the program temperature reduction box to circulate on the market, this cooling box can once accommodate 2ml cell cryopreservation tube 40, much higher than 18 of market program temperature reduction box.Cell survival rate is high: freeze-stored cell in the state of cell viability 100%, survivaling cell ratio is up to 95% or more after recovery, equal to or higher than program temperature reduction box on the market.
Description
Technical field
The present invention relates to a kind of cooling box, specially a kind of practical cell cryopreservation cooling box belongs to biological medicine heat preservation
Instrument application technical field.
Background technique
The use of existing cell gradient freezing storing box first takes out the pipe holder that freezes in box before freeze-stored cell, past
Organic solvent is added in box, then bracket is put back in box, then entire box is pre-chilled, when freezing, the cryopreservation tube that cell is housed
It is put into freezing storing box, lid is twisted, and gradually cools down, and drops to -20 DEG C from 4 DEG C, then drop to -80 DEG C, then takes out freezing storing box, opens
Cell taking-up is put into liquid nitrogen and saves by lid.
Existing cell gradient freezing storing box has the disadvantages that after bracket is put back in box, when to twist the lid off again,
The organic solvent that box is provided with is easy to spill, and the cell being stored in -80 DEG C of freezing storing box taking-up is especially moved to liquid
When in nitrogen tank, the lid of freezing storing box causes to be difficult to unscrew, if firmly unscrewing lid, be easier to due to placing in sub-zero temperature
The organic solvent for causing box to be provided with leaks out.
Summary of the invention
The object of the invention is that in order to solve existing cell gradient freezing storing box after bracket is put back in box, when
When twisting the lid off again, the organic solvent that box is provided with is easy to spill, and will especially be stored in -80 DEG C of freezing storing box
When cell taking-up is moved in liquid nitrogen container, the lid of freezing storing box causes to be difficult to unscrew, firmly unscrews lid due to placing in sub-zero temperature
If, it is easier to the problem of organic solvent for causing box to be provided with leaks out, and propose a kind of practical cell cryopreservation cooling box.
The purpose of the present invention can be achieved through the following technical solutions: a kind of practical cell cryopreservation cooling box, including master
Body container A, box cover, sealing-plug B, sealing vacuum layer and free storehouse, the main body container A are cylindrical structure, and the main body
Container A inner upper is arranged with box cover, and the box cover internal sleeve is equipped with sealing-plug B, vertical at the main body container A bosom
It is provided with the free storehouse of hollow cylindrical structure, and is provided with sealing vacuum layer between the free storehouse and main body container A, is utilized
The principle that sealing vacuum layer temperature conduction is blocked achievees the purpose that slow cooling, and the main body container A side wall two sides are respectively provided with
There is solid chuck；
Wherein, described box cover one end side wall is provided with the drop handle of hollow structure, and described sealing-plug B both sides of the upper end is all provided with
It is equipped with finger-hole；
Two solid chuck two sides are provided with attaching item, and the attaching inner wall be equidistantly vertically arranged it is several
A holder simultaneously fixes the strip clamping connection in liquid nitrogen container by several holders, prevents liquid nitrogen vapor from blowing to the cooling box
When, which shakes, and keeps the cooling box stronger when in use；
Wherein, the sleeve of hollow cylindrical structure, and described sleeve inner one end are provided on several described holders
It is provided through the moving axis of stretching of cylindrical structure, the sleeve inner is provided with and stretches stretches described in the sliding groove that moving axis matches
Axle sleeve is located inside the sliding groove, and described moving axis one end of stretching is vertically installed with rectangular folder push plate, and the folder push plate
Side wall is provided with rubber pad one, and the moving axis other end of stretching is provided with crowded support spring, and the crowded support spring one end is connected to cunning
Dynamic trench bottom；
Wherein, the junction stretched moving axis one end and squeeze support spring is provided with circular slide plate, and in the sliding groove
Portion edge is arranged with the rubber ring one of hollow cylindrical structure, increases frictional resistance when slide plate slides by rubber ring one,
Make the cooling box be not easy to shake when being blown by liquid nitrogen vapor, ensure that the stability used；
The free storehouse is hollow cylindrical structure, and storehouse partition is provided on the free storehouse, in the storehouse partition simultaneously
Row is provided with several pendulum sabots, several tubulature cylinders of spaced set, several described tubulatures in several described pendulum sabots
Cylinder is fixedly connected by cylinder latticing one between any two；
Wherein, pipe tankage is provided with inside several described tubulature cylinders, and several described dress tube walls are arranged
There is the rubber ring two of hollow ring structure.
Further technological improvement of the invention is: two solid chucks are symmetrical set, and the length of the solid chuck
Degree is less than the length of main body container A.
Further technological improvement of the invention is: slide plate outer ring edge is bonded with one inner wall of rubber ring, and sliding
The diameter of plate and the inner diameter size of rubber ring one match, and sliding groove one end exit is provided with the baffle ring of circular ring shape, passes through
To slide plate edge block, anti-limited slip plate is sliding out outer baffle ring from sliding groove.
Further technological improvement of the invention is: several described pendulum sabot both ends pass through in connecting plate item and storehouse partition
Wall is fixedly connected, and is fixedly connected by cylinder latticing two between the tubulature cylinder in several pendulum sabots, guarantees cell cryopreservation tube
The fastness put.
Further technological improvement of the invention is: the pipe tankage matches with cell cryopreservation tube, and cell cryopreservation tube
It puts inside pipe tankage, and rubber ring two is bonded with cell cryopreservation pipe outer wall, guarantees that cell cryopreservation tube is put in free storehouse
It is more firm, directly contact, hitting for cell cryopreservation tube and the storehouse partition in free storehouse is avoided, guarantee cell cryopreservation tube is being frozen
Safety when depositing.
Further technological improvement of the invention is: the bottom the main body container A is provided with circular bottom plate, and the bottom
Board bottom portion is provided with rubber pad two, during staff hangs in liquid nitrogen container and fix the cooling box, passes through main body
The rubber pad two and liquid nitrogen top tank structure contact-impact being arranged on the bottom plate of container A bottom, reduce the jitter amplitude of the cooling box,
And prevent main body container A outer wall and liquid nitrogen top tank structure collides damage.
Further technological improvement of the invention is: the finger-hole is hollow cylindrical structure, and is set inside the finger-hole
It is equipped with catching groove, the ring of pinching of hollow ring structure is provided at the catching groove upper end outlet, staff needs to make sealing-plug B
When, by the way that finger buckle is pinched pinching on ring above the catching groove of finger-hole, then applies pulling force outward and can easily draw sealing-plug B
Out, it exerts a force convenient for staff, ensure that the convenience used.
Further technological improvement of the invention is: the moving axis of stretching is slidably connected by slide plate and sliding groove, and squeezes support
Spring is flexibly connected with moving axis is stretched, and is provided crowded support force to moving axis is stretched by squeezing support spring, is enable to stretch moving axis in the sliding groove of sleeve
In stretch, be clamped by the strip of two of the back side main body container A solid chucks and liquid nitrogen top tank structure, strip is connected to two dresses
Between holder in narrow bars, crowded support force is provided to moving axis is stretched by the crowded support spring in holder, makes the rubber pad for pressing from both sides push plate side wall
One is tightly fitted on strip side wall, then compound rubber pad one provides frictional resistance, mounts the cooling box in liquid nitrogen container solid
It is fixed more firm.
The application method of the cooling box specifically includes the following steps:
Step 1: cell cryopreservation tube is inserted into the pipe tankage of the tubulature cylinder in free storehouse by staff, is made each thin
It is spaced from each other between born of the same parents' cryopreservation tube, covers sealing-plug B, be a cord on drop handle, container is hung into the liquid nitrogen in liquid nitrogen container
In steam, during staff hangs in liquid nitrogen container and fix the cooling box, pass through the bottom plate of the bottom main body container A
The rubber pad two and liquid nitrogen top tank structure contact-impact of upper setting；
Step 2: then, then pass through two solid chucks at the back side main body container A and the strip clamping of liquid nitrogen top tank structure, card
Item is connected between the holder on two attaching items, is provided crowded support force to moving axis is stretched by the crowded support spring in holder, is made to press from both sides
The rubber pad one of push plate side wall is tightly fitted on strip side wall, then compound rubber pad one provides frictional resistance, to main body container A
Be fixed, in free storehouse, due to the presence of sealing vacuum layer and sealing-plug B, the temperature of cell cryopreservation tube will from room temperature by
It is gradually slowly dropped to consistent with liquid nitrogen vapor temperature.
Compared with prior art, the beneficial effects of the present invention are:
1, extremely convenient and practical: conventional cell freezes it is generally necessary to which 4 DEG C, -20 DEG C of refrigerators and -80 DEG C of refrigerators, this container are straight
It connects and hangs in liquid nitrogen container, eliminate the demand to three kinds of refrigerators, also eliminate the step that container is transferred to different temperatures environment
Suddenly.
2, capacity is big: under identical appearance volume, compared with the program temperature reduction box to circulate on the market, this cooling box can be with one
Secondary receiving 2ml cell cryopreservation tube 40, much higher than 18 of market program temperature reduction box.
3, cell survival rate is high: freeze-stored cell in the state of cell viability 100%, survivaling cell ratio is reachable after recovery
95% or more, equal to or higher than program temperature reduction box on the market.
4, in this cooling box by being a cord on drop handle, after container is hung in the liquid nitrogen vapor of liquid nitrogen container, then
It is clamped by two solid chucks at the back side main body container A and the strip of liquid nitrogen top tank structure, strip is connected on two attaching items
Holder between, by the crowded support spring in holder to stretch moving axis provide squeeze support force, make press from both sides push plate side wall rubber pad one tightly
It is fitted on strip side wall, then compound rubber pad one provides frictional resistance, mounts the cooling box in liquid nitrogen container and fix more
It is firm, and two solid chucks are symmetrical set, several holders on two attaching items are symmetrical set, and pass through several holders
The strip clamping connection in liquid nitrogen container is fixed simultaneously, when preventing liquid nitrogen vapor from blowing to the cooling box, which shakes, and makes
The cooling box is stronger when in use, improves the cooling box to the cooling quality of cell cryopreservation tube, and prevent cell cryopreservation
It is bad that the component on the cooling box wiping clashing and breaking occur for pipe.
5, several tubulature cylinders of spaced set, the cooling box make in several pendulum sabots being arranged side by side in free storehouse
Cell cryopreservation tube is inserted into the pipe tankage of tubulature cylinder by used time, staff, makes to be spaced from each other between each cell cryopreservation tube,
It prevents from occurring to hit damage, and rubber ring two and cell cryopreservation tube inside pipe tankage when being freezed between cell cryopreservation tube
Outer wall fitting, guarantee cell cryopreservation tube put in free storehouse it is more firm, avoid the storehouse in cell cryopreservation tube and free storehouse every
The direct contact of plate is hit, and guarantees safety of the cell cryopreservation tube when being frozen, and pass through cylinder between several tubulature cylinders
Latticing one or cylinder latticing two are fixedly connected, and are put and are fixedly connected between sabot and storehouse partition by connecting plate item, guarantee cell cryopreservation tube
The fastness put.
6, during hanging and fix the cooling box in liquid nitrogen container in staff, pass through the bottom main body container A
The rubber pad two and liquid nitrogen top tank structure contact-impact being arranged on bottom plate, reduce the jitter amplitude of the cooling box, and prevent main body
Container A outer wall and liquid nitrogen top tank structure collide damage, and when staff needs to make sealing-plug B, by pinching finger buckle
Pinching on ring above to the catching groove of finger-hole, then application pulling force can easily pull out sealing-plug B outward, apply convenient for staff
Power ensure that the convenience used；The cooling box has good social benefit, is suitble to promote the use of.
Detailed description of the invention
In order to facilitate the understanding of those skilled in the art, the present invention will be further described below with reference to the drawings.
Fig. 1 is the whole three-dimensional structural perspective of the present invention.
Fig. 2 is main body container A structure schematic diagram of the present invention.
Fig. 3 is main body container A top view of the present invention.
Fig. 4 is the solid chuck front view of the present invention.
Fig. 5 is holder front view of the present invention.
Fig. 6 is holder sectional view of the present invention.
Fig. 7 is the free storehouse top view of the present invention.
Fig. 8 is present invention pendulum sabot top view.
Fig. 9 is present invention pendulum sabot front view.
Figure 10 is tubulature cylinder top view of the present invention.
Figure 11 is main body container bottom substance schematic diagram of the present invention.
Figure 12 is finger-hole sectional view of the present invention.
In figure: 1, main body container A；2, box cover；3, sealing-plug B；4, vacuum layer is sealed；5, free space；6, cell cryopreservation
Pipe；7, drop handle；8, finger-hole；9, solid chuck；10, attaching item；11, holder；12, sleeve；13, moving axis is stretched；14, push plate is pressed from both sides；15, rubber
Rubber mat one；16, sliding groove；17, support spring is squeezed；18, slide plate；19, rubber ring one；20, baffle ring；21, storehouse partition；22, sabot is put；
23, tubulature cylinder；24, cylinder latticing one；25, connecting plate item；26, cylinder latticing two；27, pipe tankage；28, rubber ring two；29, bottom plate；30,
Rubber pad two；31, catching groove；32, ring is pinched.
Specific embodiment
Technical solution of the present invention is clearly and completely described below in conjunction with embodiment, it is clear that described reality
Applying example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is general
Logical technical staff all other embodiment obtained without creative efforts belongs to what the present invention protected
Range.
It please refers to shown in Fig. 1-12, a kind of practical cell cryopreservation cooling box, including main body container A1, box cover 2, sealing-plug
B3, sealing vacuum layer 4 and free storehouse 5, main body container A1 is cylindrical structure, and main body container A1 inner upper is arranged with box cover
2,2 internal sleeve of box cover is equipped with sealing-plug B3, and the freedom of hollow cylindrical structure is vertically provided at main body container A1 bosom
Storehouse 5, and sealing vacuum layer 4 is provided between free storehouse 5 and main body container A1, it is blocked using sealing 4 temperature conduction of vacuum layer
Principle achieve the purpose that slow cooling, main body container A1 side wall two sides are provided with solid chuck 9；
Wherein, 2 one end side wall of box cover is provided with the drop handle 7 of hollow structure, and sealing-plug B3 both sides of the upper end is provided with
Finger-hole 8；
Two solid 9 two sides of chuck are provided with attaching item 10, and 10 inner wall of attaching item is equidistantly vertically arranged several
Holder 11 simultaneously fixes the strip clamping connection in liquid nitrogen container by several holders 11, prevents liquid nitrogen vapor from blowing the cooling box
When sending, which shakes, and keeps the cooling box stronger when in use；
Wherein, the sleeve 12 of hollow cylindrical structure is provided on several holders 11, and 12 inside one end of sleeve is passed through
Wear be equipped with cylindrical structure stretch moving axis 13, sleeve 12 is internally provided with and stretches the sliding groove 16 that moving axis 13 matches and stretches moving axis
13 are set in inside sliding groove 16, and stretch 13 one end of moving axis and be vertically installed with rectangular folder push plate 14, and press from both sides 14 side wall of push plate
It is provided with rubber pad 1,13 other end of moving axis is stretched and is provided with crowded support spring 17, and squeeze support 17 one end of spring and be connected to sliding groove
16 bottoms；
Wherein, the junction stretched 13 one end of moving axis and squeeze support spring 17 is provided with circular slide plate 18, and in sliding groove 16
Portion edge is arranged with the rubber ring 1 of hollow cylindrical structure, increases friction when slide plate 18 slides by rubber ring 1
Resistance makes the cooling box be not easy to shake when being blown by liquid nitrogen vapor, ensure that the stability used；
Free storehouse 5 is hollow cylindrical structure, and storehouse partition 21 is provided on free storehouse 5, is arranged side by side in storehouse partition 21
There are several to put sabot 22, several put several tubulature cylinders 23 of spaced set in sabots 22, are arranged side by side in free storehouse 5
Spaced set several tubulature cylinders 23 in several pendulum sabots 22, when in use, staff is by cell cryopreservation for the cooling box
Pipe 6 is inserted into the pipe tankage 27 of tubulature cylinder 23, makes to be spaced from each other between each cell cryopreservation tube 6, prevent cell cryopreservation tube 6 it
Between occur to hit damage when being freezed, several tubulature cylinders 23 are fixedly connected by cylinder latticing 1 between any two；
Wherein, pipe tankage 27 is provided with inside several tubulature cylinders 23, and several 23 inner walls of tubulature cylinder are arranged with
The rubber ring 2 28 of hollow ring structure.
As preferred embodiments of the present invention, two solid chucks 9 are symmetrical set, and the length of solid chuck 9 is less than main body
The length of container A 1.
As preferred embodiments of the present invention, 18 outer ring edge of slide plate is bonded with one 19 inner wall of rubber ring, and slide plate 18
The inner diameter size of diameter and rubber ring 1 match, and 16 one end exit of sliding groove is provided with the baffle ring 20 of circular ring shape,
By baffle ring 20 to 18 edge block of slide plate, anti-limited slip plate 18 is sliding out outer from sliding groove 16.
As preferred embodiments of the present invention, several pendulum 22 both ends of sabot pass through connecting plate item 25 and 21 inner wall of storehouse partition
It is fixedly connected, and is fixedly connected by cylinder latticing 2 26 between the tubulature cylinder 23 in several pendulum sabots 22, guarantee that cell freezes
Deposit the fastness that pipe 6 is put.
As preferred embodiments of the present invention, pipe tankage 27 matches with cell cryopreservation tube 6, and cell cryopreservation tube 6 is put
To inside pipe tankage 27, and rubber ring 2 28 is bonded with 6 outer wall of cell cryopreservation tube, guarantees that cell cryopreservation tube is put in free storehouse 5
It is more firm to put, and avoids directly contact, hitting for cell cryopreservation tube 6 and the storehouse partition 21 in free storehouse 5, guarantee cell cryopreservation tube
27 safety when being frozen.
As preferred embodiments of the present invention, the bottom main body container A1 is provided with circular bottom plate 29, and 29 bottom of bottom plate
It is provided with rubber pad 2 30, during staff hangs in liquid nitrogen container and fix the cooling box, passes through main body container
The rubber pad 2 30 and liquid nitrogen top tank structure contact-impact being arranged on the bottom plate 29 of the bottom A1, reduce the shake width of the cooling box
Degree, and prevent main body container A1 outer wall and liquid nitrogen top tank structure collides damage.
As preferred embodiments of the present invention, finger-hole 8 is hollow cylindrical structure, and finger-hole 8 is internally provided with catching groove 31,
The ring 32 of pinching that hollow ring structure is provided at 31 upper end outlet of catching groove passes through when staff needs to make sealing-plug B3
Finger buckle is pinched into pinching on ring 32 above the catching groove 31 of finger-hole 8, then apply pulling force outward can easily pull out sealing-plug B3,
Convenient for staff's force, the convenience used ensure that.
It as preferred embodiments of the present invention, stretches moving axis 13 and is slidably connected by slide plate 18 with sliding groove 16, and squeeze support bullet
Spring 17 is flexibly connected with moving axis 13 is stretched, and is provided crowded support force to moving axis 13 is stretched by squeezing support spring 17, is enable to stretch moving axis 13 in sleeve
It stretches, is clamped by the strip of two solid chucks 9 and liquid nitrogen top tank structure at the back side main body container A, strip in 12 sliding groove 16
It is connected between the holder 11 on two attaching items 10, provides crowded support to moving axis 13 is stretched by the crowded support spring 17 in holder 11
Power is fitted in the rubber pad 1 for pressing from both sides 14 side wall of push plate tightly on strip side wall, then compound rubber pad 1 provides friction resistance
Power makes the cooling box mount fixation in liquid nitrogen container more firm.
The application method of the cooling box specifically includes the following steps:
Step 1: in the pipe tankage 27 for the tubulature cylinder 23 that cell cryopreservation tube 6 is inserted into free storehouse 5 by staff, make
It is spaced from each other between each cell cryopreservation tube 6, covers sealing-plug B3, be a cord on drop handle 7, container is hung in liquid nitrogen
In the liquid nitrogen vapor of tank, during staff hangs in liquid nitrogen container and fix the cooling box, pass through main body container A1
The rubber pad 2 30 and liquid nitrogen top tank structure contact-impact being arranged on the bottom plate 29 of bottom；
Step 2: then, then the strip clamping of two solid chucks 9 and liquid nitrogen top tank structure by the back side main body container A1,
Strip is connected between the holder 11 on two attaching items 10, is provided by the crowded support spring 17 in holder 11 moving axis 13 is stretched
Support force is squeezed, is fitted in the rubber pad 1 for pressing from both sides 14 side wall of push plate tightly on strip side wall, then the offer of compound rubber pad 1 rubs
Resistance is wiped, main body container A1 is fixed, in free storehouse 5, due to the presence of sealing vacuum layer 4 and sealing-plug B3, cell
The temperature of cryopreservation tube 6 will gradually be slowly dropped to consistent with liquid nitrogen vapor temperature from room temperature.
Compared with prior art, the beneficial effects of the present invention are:
1, extremely convenient and practical: conventional cell freezes it is generally necessary to which 4 DEG C, -20 DEG C of refrigerators and -80 DEG C of refrigerators, this container are straight
It connects and hangs in liquid nitrogen container, eliminate the demand to three kinds of refrigerators, also eliminate the step that container is transferred to different temperatures environment
Suddenly.
2, capacity is big: under identical appearance volume, compared with the program temperature reduction box to circulate on the market, this cooling box can be with one
Secondary receiving 2ml cell cryopreservation tube 40, much higher than 18 of market program temperature reduction box.
3, cell survival rate is high: freeze-stored cell in the state of cell viability 100%, survivaling cell ratio is reachable after recovery
95% or more, equal to or higher than program temperature reduction box on the market.
4, in this cooling box by being a cord on drop handle 7, after container is hung in the liquid nitrogen vapor of liquid nitrogen container, then
It is clamped by the strip of two solid chucks 9 and liquid nitrogen top tank structure at the back side main body container A, strip is connected to two attaching items 10
On holder 11 between, support force is squeezed to stretching moving axis 13 and provide by crowded support spring 17 in holder 11, makes to press from both sides 14 side wall of push plate
Rubber pad 1 is tightly fitted on strip side wall, then compound rubber pad 1 provides frictional resistance, makes the cooling box in liquid nitrogen
It is more firm that fixation is mounted in tank, and two solid chucks 9 are symmetrical set, several holders 11 on two attaching items 10 are in pair
Claim setting, the strip clamping connection in liquid nitrogen container is fixed simultaneously by several holders 11, prevents liquid nitrogen vapor from blowing the cooling box
When sending, which shakes, and keeps the cooling box stronger when in use, improves the cooling box to cell cryopreservation tube 6
Cooling quality, and prevent cell cryopreservation tube 6 on the cooling box component occur wipe clashing and breaking it is bad.
5, several tubulature cylinders 23 of spaced set, the cooling in several pendulum sabots 22 being arranged side by side in free storehouse 5
When in use, cell cryopreservation tube 6 is inserted into the pipe tankage 27 of tubulature cylinder 23 box by staff, makes each cell cryopreservation tube 6
Between be spaced from each other, prevent between cell cryopreservation tube 6 occurring to hit damage, and the rubber inside pipe tankage 27 when being freezed
It encloses 2 28 to be bonded with 6 outer wall of cell cryopreservation tube, it is more firm that guarantee cell cryopreservation tube is put in free storehouse 5, avoids cell
Cryopreservation tube 6 and the storehouse partition 21 in free storehouse 5 directly contacts, are hit, safety of the guarantee cell cryopreservation tube 27 when being frozen,
And be fixedly connected by cylinder latticing 1 or cylinder latticing 2 26 between several tubulature cylinders 23, pendulum sabot 22 and storehouse partition 21 it
Between be fixedly connected by connecting plate item 25, guarantee the fastness that cell cryopreservation tube 6 is put.
6, during hanging and fix the cooling box in liquid nitrogen container in staff, pass through the bottom main body container A1
The rubber pad 2 30 and liquid nitrogen top tank structure contact-impact being arranged on bottom plate 29, reduce the jitter amplitude of the cooling box, and prevent
Main body container A1 outer wall and liquid nitrogen top tank structure collide damage, and when staff needs to make sealing-plug B3, by by hand
Refer to that button pinches pinching on ring 32 above the catching groove 31 of finger-hole 8, then apply pulling force outward can easily pull out sealing-plug B3, is convenient for
Staff's force, ensure that the convenience used；The cooling box has good social benefit, is suitble to promote the use of.
Working principle: cell cryopreservation is intracellular water is extracellular by infiltration discharge, reduction and resistance in slow cooling
The process of disconnected cell metabolism, and slow cooling is a uncontrollable process, this container is hindered using vacuum layer temperature conduction
Disconnected principle achievees the purpose that slow cooling, when in use, firstly, cell cryopreservation tube 6 is inserted into free storehouse 5 by staff
Tubulature cylinder 23 pipe tankage 27 in, make to be spaced from each other between each cell cryopreservation tube 6, cover sealing-plug B3, be on drop handle 7
The cooling is hung in liquid nitrogen container and fixed to a piece cord in staff by container hanging in the liquid nitrogen vapor of liquid nitrogen container
During box, the rubber pad 2 30 and liquid nitrogen top tank structure contact-impact being arranged on the bottom plate 29 by the bottom main body container A1,
Then, then by the strip of two solid chucks 9 and liquid nitrogen top tank structure at the back side main body container A1 it is clamped, strip is connected to two
Between holder 11 on attaching item 10, crowded support force is provided to moving axis 13 is stretched by the crowded support spring 17 in holder 11, makes to press from both sides push plate
The rubber pad 1 of 14 side walls is tightly fitted on strip side wall, then compound rubber pad 1 provides frictional resistance, holds to main body
Device A1 is fixed, and in free storehouse 5, due to the presence of sealing vacuum layer 4 and sealing-plug B3, the temperature of cell cryopreservation tube 6 will
It is gradually slowly dropped to from room temperature consistent with liquid nitrogen vapor temperature.
Present invention disclosed above preferred embodiment is only intended to help to illustrate the present invention.There is no detailed for preferred embodiment
All details are described, are not limited the invention to the specific embodiments described.Obviously, according to the content of this specification,
It can make many modifications and variations.These embodiments are chosen and specifically described to this specification, is in order to better explain the present invention
Principle and practical application, so that skilled artisan be enable to better understand and utilize the present invention.The present invention is only
It is limited by claims and its full scope and equivalent.
Claims (9)
1. a kind of practical cell cryopreservation cooling box, it is characterised in that: including main body container A (1), box cover (2), sealing-plug B
(3), vacuum layer (4) and free storehouse (5) are sealed, the main body container A (1) is cylindrical structure, and the main body container A (1)
Inner upper is arranged with box cover (2), and box cover (2) internal sleeve is equipped with sealing-plug B (3), in main body container A (1) inside
Between be vertically provided with the free storehouse (5) of hollow cylindrical structure, and be arranged between the free storehouse (5) and main body container A (1)
Have sealing vacuum layer (4), main body container A (1) the side wall two sides are provided with solid chuck (9)；
Wherein, described box cover (2) one end side wall is provided with the drop handle (7) of hollow structure, and the sealing-plug B (3) upper surface two
Side is provided with finger-hole (8)；
Two described solid chuck (9) two sides are provided with attaching item (10), and the attaching item (10) inner wall is equidistant vertical
Several holders (11) are set；
Wherein, the sleeve (12) of hollow cylindrical structure, and the sleeve (12) are provided on several described holders (11)
Internal one end is provided through stretching moving axis (13) for cylindrical structure, and the sleeve (12) is internally provided with and stretches moving axis (13) phase
Matched sliding groove (16) moving axis (13) of stretching is set in the sliding groove (16) inside, and described moving axis (13) one end of stretching is hung down
It is directly provided with rectangular folder push plate (14), and folder push plate (14) side wall is provided with rubber pad one (15), it is described to stretch moving axis
(13) other end, which is provided with, squeezes support spring (17), and described crowded support spring (17) one end is connected to sliding groove (16) bottom；
Wherein, the junction stretched moving axis (13) one end and squeeze support spring (17) is provided with circular slide plate (18), and described
The rubber ring one (19) of hollow cylindrical structure is arranged at sliding groove (16) internal edge；
The free storehouse (5) is hollow cylindrical structure, and storehouse partition (21) is provided on the free storehouse (5), the storehouse every
Being arranged side by side in plate (21) has several to put sabot (22), several tubulatures of spaced set on several described pendulum sabots (22)
Cylinder (23), several described tubulature cylinders (23) are fixedly connected by cylinder latticing one (24) between any two；
Wherein, it is provided with pipe tankage (27) inside several described tubulature cylinders (23), and in several described tubulature cylinders (23)
Wall is arranged with the rubber ring two (28) of hollow ring structure.
2. a kind of practical cell cryopreservation cooling box according to claim 1, which is characterized in that two solid chucks
(9) it is symmetrical set, and the length of solid chuck (9) is less than the length of main body container A (1).
3. a kind of practical cell cryopreservation cooling box according to claim 1, which is characterized in that slide plate (18) outer ring
Edge is bonded with rubber ring one (19) inner wall, and the inner diameter size of the diameter and rubber ring one (19) of slide plate (18) matches,
And sliding groove (16) one end exit is provided with the baffle ring (20) of circular ring shape.
4. a kind of practical cell cryopreservation cooling box according to claim 1, which is characterized in that several described pendulum sabots
(22) both ends pass through connecting plate item (25) and are fixedly connected with storehouse partition (21) inner wall, and the tubulature cylinder on several pendulum sabot (22)
(23) it is fixedly connected by cylinder latticing two (26) between.
5. a kind of practical cell cryopreservation cooling box according to claim 1, which is characterized in that the pipe tankage (27) with
Cell cryopreservation tube (6) matches, and cell cryopreservation tube (6) puts pipe tankage (27) inside, and rubber ring two (28) and cell
The fitting of cryopreservation tube (6) outer wall.
6. a kind of practical cell cryopreservation cooling box according to claim 1, which is characterized in that the main body container A (1)
Bottom is provided with circular bottom plate (29), and the bottom plate (29) bottom is provided with rubber pad two (30).
7. a kind of practical cell cryopreservation cooling box according to claim 1, which is characterized in that during the finger-hole (8) is
Hollow-cylindrical structure, and the finger-hole (8) is internally provided with catching groove (31), is provided at catching groove (31) upper end outlet hollow
Cirque structure pinches ring (32).
8. a kind of practical cell cryopreservation cooling box according to claim 1, which is characterized in that described stretch moving axis (13) lead to
It crosses slide plate (18) to be slidably connected with sliding groove (16), and squeezes support spring (17) and be flexibly connected with moving axis (13) is stretched.
9. a kind of practical cell cryopreservation cooling box according to claim 1, which is characterized in that the user of the cooling box
Method specifically includes the following steps:
Step 1: cell cryopreservation tube (6) is inserted into the pipe tankage (27) of the tubulature cylinder (23) in free storehouse (5) by staff
In, make to be spaced from each other between each cell cryopreservation tube (6), cover sealing-plug B (3), is a cord on drop handle (7), will hold
Device is hung in the liquid nitrogen vapor of liquid nitrogen container, during staff hangs in liquid nitrogen container and fix the cooling box, is led to
Cross the rubber pad two (30) and liquid nitrogen top tank structure contact-impact being arranged on the bottom plate (29) of main body container A (1) bottom；
Step 2: two solid chuck (9) and the strip clamping of liquid nitrogen top tank structure then, then by main body container A (1) back side,
Strip is connected between the holder (11) on two attaching items (10), by the crowded support spring (17) in holder (11) to stretching
Axis (13), which provides, squeezes support force, is fitted in the rubber pad one (15) for pressing from both sides push plate (14) side wall tightly on strip side wall, then cooperate rubber
Rubber mat one (15) provides frictional resistance, and main body container A (1) is fixed, in free storehouse (5), due to sealing vacuum layer (4)
And the presence of sealing-plug B (3), the temperature of cell cryopreservation tube (6) will gradually be slowly dropped to consistent with liquid nitrogen vapor temperature from room temperature.
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| CN109997838B CN109997838B (en) | 2021-10-08 |
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| CN111919838A (en) * | 2020-09-03 | 2020-11-13 | 林彩琴 | Utilize fixed cell cryopreserving cooling box of atmospheric pressure |
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| CN115968865A (en) * | 2023-02-01 | 2023-04-18 | 北京赛赋医药研究院有限公司 | CAR-T cell cryopreservation box and cryopreservation method |
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| CN115968865A (en) * | 2023-02-01 | 2023-04-18 | 北京赛赋医药研究院有限公司 | CAR-T cell cryopreservation box and cryopreservation method |
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| CN109997838B (en) | 2021-10-08 |
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