CN109991412B - A kind of method of capsicum alkali composition in detection grease - Google Patents
A kind of method of capsicum alkali composition in detection grease Download PDFInfo
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- CN109991412B CN109991412B CN201811504297.XA CN201811504297A CN109991412B CN 109991412 B CN109991412 B CN 109991412B CN 201811504297 A CN201811504297 A CN 201811504297A CN 109991412 B CN109991412 B CN 109991412B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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Abstract
The invention discloses a kind of methods of capsicum alkali composition in detection grease, method includes the following steps: carrying out separation and Extraction to the capsicum base molecule in grease using affine in immunity method in advance, then using immunochromatographic method to separation and Extraction to capsicum base molecule detect, it is described that the capsicum base molecule progress separation and Extraction in grease is referred to using the solution dilution oil sample containing methanol, nano-titanium dioxide using affine in immunity method, then add the immunomagnetic ca pture capsicum base molecule therein in conjunction with capsaicine molecular specificity.This method combines immunochromatography technique with affine in immunity technology, significantly reduces the workload that capsaicine detects in grease, and improve the sensitivity of detection.
Description
Technical field
The invention belongs to field of biotechnology, and in particular to a method of capsicum alkali composition in detection grease.
Background technique
" gutter oil " is also referred to as " swill oil " " hogwash fat ", refers to all kinds of poor oils present in life, mainly by discarding
Grease is through precipitating impurity elimination, and a kind of quality of deodorization decoloring is very poor, extremely antihygienic unedible oil.But some illegal retailers are by interests
It drives and ignores people's life's safety, produce and process " gutter oil " privately and as edible oil sale at low prices to cafe,
Health and life security to consumer bring serious harm.However, detection gutter oil is extremely difficult.Gutter oil is
One extremely non-type research object, the composition that do not fix bring very big do not know to detection also without typical sample
Property.Ingredient complicated and changeable leads to not find characteristic index for gutter oil, and the characteristic index of normal edible oil can not yet
It is applicable in the detection of gutter oil, brings very big difficulty for the judgement of gutter oil.
Capsicum appears widely in various dish as the common seasoning of Chinese, occurs especially in Sichuan cuisine more,
And the capsaicine in capsicum is a kind of substance for being soluble in grease, is difficult to effectively remove completely by simply extracting.Therefore, it examines
The capsaicine surveyed in grease becomes one of the option for identifying oil quality.Currently, the method for detection capsaicine mainly has light splitting light
Degree method, chromatography, sensor method, immunological method etc., but these methods still generally existing complex pretreatment, what time-consuming
Problem, and sensitivity also tends to the requirement that can not meet polished fat's detection.
Summary of the invention
The present invention provides a kind of easy-to-use detection with regard to this problem of the capsaicine time-consuming and laborious, inefficiency of detection in grease
Method.
The technical problems to be solved by the invention are achieved by the following technical programs:
A kind of method of capsicum alkali composition in detection grease, method includes the following steps: using affine in immunity method in advance
Separation and Extraction is carried out to the capsicum base molecule in grease, the capsicum base molecule that then separation and Extraction is arrived using immunochromatographic method into
Row detection, it is described using affine in immunity method in grease capsicum base molecule carry out separation and Extraction refer to using containing methanol, receive
The solution of rice titanium dioxide dilutes oil sample, and then immunomagnetic ca pture of the addition in conjunction with capsaicine molecular specificity be wherein
Capsicum base molecule.
The method for detecting capsicum alkali composition among having as described above, the partial size of the titanium dioxide are 2nm-25nm
The method of capsicum alkali composition, immunochromatographic method refer to using immunity-chromatography test in a kind of detection grease as described above
Paper slip is detected, the immuno-chromatographic test paper strip include successively overlap joint paste sample pad on the bottom plate of PVC material, chromatography
Film, blotting paper have detection zone and quality control region in chromatographic film, and detection zone is coated with capsaicine-carrier protein couplet object.
The method of capsicum alkali composition, the immunomagnetic beads use 5- ammonia in the preparation in a kind of detection grease as described above
Base valeric acid is closed.
The method of capsicum alkali composition, the immunomagnetic beads during the preparation process, are adopted in a kind of detection grease as described above
With the redissolution liquid resuspended particle containing lactoalbumin hydrolysate.
The method of capsicum alkali composition in a kind of detection grease as described above, after immunomagnetic ca pture capsicum base molecule,
Immunomagnetic beads are resuspended with the re-suspension liquid containing S9 surfactant (Tetronic 1307).
The invention has the following beneficial effects:
Grease has the characteristics that sticky, and the chromatographic property on immuno-chromatographic test paper strip is poor, therefore, in detection grease
Ingredient or extract target substance detected again (extraction leads to the cumbersome of detection method) or to grease into
Row significantly dilutes is detected (reduction that dilution leads to detection sensitivity) again.Existing immunochromatographic method is needed from large volume
Grease in extract, then to carry out drying operation to by the organic solvent of large volume, complex steps, time-consuming (need several small
When, even more long), instrument is relied on high, be not easy to high-throughput detection.In a kind of detection grease provided by the invention capsaicine at
The method divided, this method are first enriched with the capsicum base molecule in grease with immunomagnetic beads, solve many and diverse pre-treatment
(use in conjunction of methanol and nano-titanium dioxide both solved the problems, such as the sticky of grease to problem, it is ensured that resists on immunomagnetic beads
The activity of body).Secondly, quickly detecting using immunochromatographic method, testing cost is reduced, shortens detection time.It solves existing
Having in grease the problem of time-consuming and laborious capsaicine detection, inefficiency, (entire testing process gets off, it is only necessary to 70min or even shorter
Time；Detection demand can be met by only needing to handle small samples, be provided convenience for high-throughput detection).
Specific embodiment
With embodiment, the present invention will be described in detail below, and the examples are only preferred embodiments of the present invention, no
It is limitation of the invention.
1 sample pre-treatments scheme of embodiment
1. the preparation of immunomagnetic beads:
(1) 1ml magnetic bead (10mg/ml, article No. MS300/Carboxyl, Japanese JSR company) is taken, magnetic bead is placed in magnetic field
In, magnetic bead is collected, supernatant is discarded；
(2) 1ml PBS(phosphate buffer solution, pH7.4 are used) magnetic bead is resuspended, 5mg EDC (1- (3- dimethylamino is added
Propyl) -3- ethyl-carbodiimide hydrochloride), room temperature shakes 15min；
(3) magnetic bead is placed in magnetic field, collects magnetic bead, remove supernatant；With 1ml PBS(pH7.4) resuspended particle；Repeat this
Step 1 time；
(4) capsazepine antibody 20mg is added, room temperature mixes 2h；
(5) sealer, the reaction was continued 2h is added；
(6) magnetic bead is placed in magnetic field, collects magnetic bead, remove supernatant；With 1ml PBS(pH7.4) resuspended particle；Repeat this
Step 2 time；
(7) liquid resuspended particle is redissolved with 10ml, is placed in 4 DEG C of preservations.
2. taking 2g grease, 8ml sample diluting liquid is added, 40 μ L immunomagnetic beads are added, rotation mixes 1h(20 DEG C -40 DEG C,
Recommend 37 DEG C).
3. the mixing solution of step 2 is placed in magnetic field, immunomagnetic beads are collected.
4. using phosphate buffer of the 1ml PBST(containing Tween-20, pH7.4, (W/V, quality are dense for polysorbas20 content 0.1%
Degree)) washing magnetic bead 1 time, and particle is suspended in 200 μ L re-suspension liquids, it is spare.
5. the preparation of test strips:
(1) according to the capsaicine-BSA conjugate for being 1.5mg/mL according to 1 μ L/cm spraying concentration, as detection zone, according to
1 μ L/cm spraying concentration is the sheep anti-mouse igg of 0.2mg/ml as quality control region, is placed in room temperature and dries, spare.
(2) sample pad, chromatographic film, blotting paper are successively overlapped and paste PVC(polyvinyl chloride) on the bottom plate of material, chromatography
The detection zone of film cuts into the immuno-chromatographic test paper strip of 4mm wide close to sample pad, quality control region close to blotting paper detection line.
6. being stored at room temperature 10min in the magnetic bead to suspend in immuno-chromatographic test paper strip sample pad end inserting step 4.
7. interpretation result:
A, can with the naked eye direct interpretation: (1) detection zone signal be better than quality control region signal, illustrates without containing capsaicine, or
The content of capsaicine is lower than the detection limit of this method.(2) detection zone signal is equal to or is weaker than quality control region signal, illustrates to contain in sample
There is capsicum alkali composition.
B, detection can also be scanned to test strips with magnetic signal detector.Equally, (1) detection zone signal is better than matter
Area's signal is controlled, illustrates the detection limit for being lower than this method without containing the content of capsaicine or capsaicine.(2) detection zone signal is equal to
Or it is weaker than quality control region signal, illustrate to contain capsicum alkali composition in sample.
Embodiment 2
Influence of the immunomagnetic beads sealer for testing result: based on embodiment 1, using different sealer (see
Table 1) closing magnetic bead.Different sealers has apparent influence to detection sensitivity, and 5- aminovaleric acid Monitoring lower-cut is best, deep water
Isinglass is then worst.Closed particle is carried out with Tris, and aggregation is then easy to happen in oil water mixture, especially in magnetic field,
It is difficult to break up after aggregation, causes next detection operation that can not carry out.
Influence of 1 sealer of table for result
| Sealer | Sealer working concentration (final concentration) | Monitoring lower-cut (μ g/kg) | Remarks |
| BSA(bovine serum albumin(BSA)) | 1%(W/V) | 60 | |
| Deep water isinglass (sigma company) | 0.2%(W/V) | 240 | |
| PEG20000 (PEG 20000) | 1%(W/V) | 30 | |
| Tris(trishydroxymethylaminomethane) | 20mM | / | It is difficult to be resuspended after capsaicine in immunomagnetic beads adsorption sample |
| It recombinates Streptavidin (Hangzhou knob dragon product) | 10mg/ml | 7.5 | |
| 5- aminovaleric acid | 0.5%(W/V) | 1.9 |
Embodiment 3
Immunomagnetic beads redissolve influence of the liquid for testing result: based on embodiment 1, comparing different redissolution liquid (tables
2) for the influence of testing result, as can be seen from the table, it is added to the redissolution liquid of lactoalbumin hydrolysate, for detection sensitivity
Promotion has clear improvement.
Table 2 redissolves influence of the liquid for testing result
| Number | Formula | Monitoring lower-cut (μ g/kg) |
| 1 | 20mM Tris-HCl, 1% BSA, pH7.5 | 6 |
| 2 | PBS, 1%BSA, pH7.4 | 24 |
| 3 | 20mM Tris-HCl, pH7.5 | 3 |
| 4 | PBS, 1% lactoalbumin hydrolysate, pH7.4 | 1.5 |
Embodiment 4
Influence of the sample treatment for testing result: based on embodiment 1, different sample diluting liquids are compared
The influence of (table 3- table 6) for testing result, as can be seen from the table, methanol and nano-titanium dioxide can be effectively improved inspection
The sensitivity of survey, and the two use in conjunction better effect.The concentration of methanol, nano-titanium dioxide, the partial size of nano-titanium dioxide
These factors can all influence Monitoring lower-cut.
Influence of 3 sample diluting liquid of table for testing result
| Number | Formula | Monitoring lower-cut (μ g/kg) |
| 1 | PBS, pH7.4 | 100 |
| 2 | Contain 20%(V/V) PBS, pH7.4 of methanol | 12.5 |
| 3 | 10mg/ml nano-titanium dioxide suspension (is suspended in PBS, pH7.4) | 6.25 |
| 4 | 10mg/ml nano-titanium dioxide suspension (is suspended in the PBS containing 20% (V/V) methanol, pH7.4) | 1.5 |
Influence of the methanol content for testing result in 4 sample diluting liquid of table
| Number | Formula | Monitoring lower-cut (μ g/kg) |
| 1 | 10mg/ml nano-titanium dioxide suspension (is suspended in the PBS containing 10% (V/V) methanol, pH7.4) | 6 |
| 2 | 10mg/ml nano-titanium dioxide suspension (is suspended in the PBS containing 20% (V/V) methanol, pH7.4) | 1.5 |
| 3 | 10mg/ml nano-titanium dioxide suspension (is suspended in the PBS containing 40% (V/V) methanol, pH7.4) | 6(negative sample signal is weak) |
| 4 | 10mg/ml nano-titanium dioxide suspension (is suspended in the PBS containing 60% (V/V) methanol, pH7.4) | Negative sample is without detection signal |
| 5 | 10mg/ml nano-titanium dioxide suspension (is suspended in the PBS containing 80% (V/V) methanol, pH7.4) | Negative sample is without detection signal |
Influence of the titanium dioxide nanoparticle concentration for testing result in 5 sample diluting liquid of table
| Number | Formula | Monitoring lower-cut (μ g/kg) |
| 1 | 5mg/ml nano-titanium dioxide suspension (is suspended in the PBS containing 20% (V/V) methanol, pH7.4) | 6 |
| 2 | 10mg/ml nano-titanium dioxide suspension (is suspended in the PBS containing 20% (V/V) methanol, pH7.4) | 1.5 |
| 3 | 20mg/ml nano-titanium dioxide suspension (is suspended in the PBS containing 20% (V/V) methanol, pH7.4) | 1.5 |
| 4 | 40mg/ml nano-titanium dioxide suspension (is suspended in the PBS containing 20% (V/V) methanol, pH7.4) | 1.5 |
| 5 | 60mg/ml nano-titanium dioxide suspension (is suspended in the PBS containing 20% (V/V) methanol, pH7.4) | 1.5 |
Influence of the titanium dioxide nanoparticle partial size for testing result in 6 sample diluting liquid of table
| Number | Formula | Monitoring lower-cut (μ g/kg) |
| 1 | 10mg/ml nano-titanium dioxide (200nm-400nm) suspension (is suspended in the PBS containing 20% (V/V) methanol, pH7.4) | 3 |
| 2 | 10mg/ml nano-titanium dioxide (10nm-25nm) suspension (is suspended in the PBS containing 20% (V/V) methanol, pH7.4) | 1.5 |
| 3 | 10mg/ml nano-titanium dioxide (50nm-250nm) suspension (is suspended in the PBS containing 20% (V/V) methanol, pH7.4) | 3 |
| 4 | 10mg/ml nano-titanium dioxide (400nm-1000nm) suspension (is suspended in the PBS containing 20% (V/V) methanol, pH7.4) | 3 |
| 5 | 10mg/ml nano-titanium dioxide (2nm-10nm) suspension (is suspended in the PBS containing 20% (V/V) methanol, pH7.4) | 1.5 |
Embodiment 5
Influence of the re-suspension liquid for testing result: based on embodiment 1, different re-suspension liquids (table 7) are compared for inspection
The influence of result is surveyed, as can be seen from the table, different re-suspension liquids influence the sensitivity of detection and little, but for negative sample
This detection signal has a significant impact, and the re-suspension liquid for being added to 0.5% S9 has clear improvement for detecting the promotion of signal.
Influence of 7 re-suspension liquid of table for testing result
| Number | Formula | Monitoring lower-cut (μ g/kg) | Remarks (+negative sample signal strength is represented ,+more representation signals are stronger) |
| 1 | PBS, pH7.4 | 1.5 | + |
| 2 | PBS, 1% (W/V) Tween 20, pH7.4 | 1.5 | ++ |
| 3 | PBS, 0.5% (W/V) S9, pH7.4 | 1.5 | +++ |
| 4 | PBS, 1% (W/V) S17(RHODASURF ON-870), pH7.4 | 1.5 | ++ |
Embodiment 6
1. the preparation of immunomagnetic beads:
(1) 1ml magnetic bead (10mg/ml, article No. MS300/Carboxyl, Japanese JSR company) is taken, magnetic bead is placed in magnetic field
In, magnetic bead is collected, supernatant is discarded；
(2) 1ml PBS(phosphate buffer solution, pH7.4 are used) magnetic bead is resuspended, 5mg EDC (1- (3- dimethylamino is added
Propyl) -3- ethyl-carbodiimide hydrochloride), room temperature shakes 15min；
(3) magnetic bead is placed in magnetic field, collects magnetic bead, remove supernatant；With 1ml PBS(pH7.4) resuspended particle；Repeat this
Step 1 time；
(4) capsazepine antibody 20mg is added, room temperature mixes 2h；
(5) sealer (5- aminovaleric acid, working concentration 0.5%(W/V) is added), the reaction was continued 2h；
(6) magnetic bead is placed in magnetic field, collects magnetic bead, remove supernatant；With 1ml PBS(pH7.4) resuspended particle；Repeat this
Step 2 time；
(7) liquid (PBS, 1% lactoalbumin hydrolysate, pH7.4) resuspended particle is redissolved with 10ml, is placed in 4 DEG C of preservations.
2. taking 2g grease, 8ml sample diluting liquid is added, and (10mg/ml nano-titanium dioxide suspension (is suspended in containing 20%
(V/V) in the PBS of methanol, pH7.4), 40 μ L immunomagnetic beads are added, rotation mixes 1h(and recommends 37 DEG C).
3. the mixing solution of step 2 is placed in magnetic field, immunomagnetic beads are collected.
4. using phosphate buffer of the 1ml PBST(containing Tween-20, pH7.4, (W/V, quality are dense for polysorbas20 content 0.1%
Degree)) washing magnetic bead 1 time, and particle is suspended in 200 μ L re-suspension liquids (PBS, 0.5% (W/V) S9, pH7.4), it is spare.
5. the preparation of test strips:
(1) according to the capsaicine-BSA conjugate for being 1.5mg/mL according to 1 μ L/cm spraying concentration, as detection zone, according to
1 μ L/cm spraying concentration is the sheep anti-mouse igg of 0.2mg/ml as quality control region, is placed in room temperature and dries, spare.
(2) sample pad, chromatographic film, blotting paper are successively overlapped and paste PVC(polyvinyl chloride) on the bottom plate of material, chromatography
The detection zone of film cuts into the immuno-chromatographic test paper strip of 4mm wide close to sample pad, quality control region close to blotting paper detection line.
6. being stored at room temperature 10min in the magnetic bead to suspend in immuno-chromatographic test paper strip inserting step 4.
7. interpretation result:
A, can with the naked eye direct interpretation: (1) detection zone signal be better than quality control region signal, illustrates without containing capsaicine, or
The content of capsaicine is lower than the detection limit of this method.(2) detection zone signal is equal to or is weaker than quality control region signal, illustrates to contain in sample
There is capsicum alkali composition.
B, detection can also be scanned to test strips with magnetic signal detector.Equally, (1) detection zone signal is better than matter
Area's signal is controlled, illustrates the detection limit for being lower than this method without containing the content of capsaicine or capsaicine.(2) detection zone signal is equal to
Or it is weaker than quality control region signal, illustrate to contain capsicum alkali composition in sample.
Comparative example 1
Prior art is (referring to " edible vegetable oil xenobiotic pollutants capsaicine and Aspergillus flavus toxin immuno detection technique
Study Yang Qingqing .2016 ")
Reconciliation 100 g of edible vegetable oil is weighed, after 800 mL methanol are added, concussion is mixed 1 minute, then in 50 DEG C of conditions
Lower ultrasonic extraction 10 minutes, after being cooled to room temperature, 4 DEG C, 4000 rpm centrifugation 5 minutes take 400 upper layer m L methanol phases,
It is dried with nitrogen, obtains 5 times of concentrated extracting solutions after adding 10 mL, 10% methanol-PBS to redissolve.With colloidal gold immunochromatographimethod method to mentioning
Liquid is taken to be detected.
Contrast method is (referring to the national standard (survey of GB/T 30388-2013 capsicum and its total capsicum alkali content of oleoresin
Determine high performance liquid chromatography) carry out).
23 points of oil samples, testing result such as table 8 are detected with three embodiment 6, comparative example 1 and contrast method schemes respectively
It is shown, technical solution of the present invention embodiment 6 with to compare scheme C testing result completely the same, and prior art comparative example 1 is then
There are the 3 official holidays positive, 2 missing inspections (false negative).
Table 8 detects oil sample
Embodiments of the present invention above described embodiment only expresses, the description thereof is more specific and detailed, but can not
Therefore limitations on the scope of the patent of the present invention are interpreted as, as long as skill obtained in the form of equivalent substitutions or equivalent transformations
Art scheme should all be fallen within the scope and spirit of the invention.
Claims (1)
1. it is a kind of detection grease in capsicum alkali composition method, which is characterized in that itself the following steps are included:
1. the preparation of immunomagnetic beads:
(1) 1ml magnetic bead is taken, magnetic bead is placed in magnetic field, magnetic bead is collected, discards supernatant；
(2) magnetic bead is resuspended with 1ml PBS, 5mg EDC is added, room temperature shakes 15min；
(3) magnetic bead is placed in magnetic field, collects magnetic bead, remove supernatant；With 1ml PBS resuspended particle；It repeats this step 1 time；
(4) capsazepine antibody 20mg is added, room temperature mixes 2h；
(5) sealer 5- aminovaleric acid, working concentration 0.5%(W/V is added), the reaction was continued 2h；
(6) magnetic bead is placed in magnetic field, collects magnetic bead, remove supernatant；With 1ml PBS resuspended particle；It repeats this step 2 times；
(7) liquid resuspended particle is redissolved with 10ml, is placed in 4 DEG C of preservations, wherein redissolving liquid is PBS, 1% lactoalbumin hydrolysate, pH7.4；
2. taking 2g grease, 8ml sample diluting liquid is added, 40 μ L immunomagnetic beads are added, rotation mixes 1h；Wherein, the sample is dilute
Release liquid are as follows: 10mg/ml nano-titanium dioxide suspension is suspended in the PBS containing 20% (V/V) methanol, pH7.4；
3. the mixing solution of step 2 is placed in magnetic field, immunomagnetic beads are collected；
4. being washed magnetic bead 1 time with 1ml PBST, and particle is suspended in 200 μ L re-suspension liquids, it is spare；Wherein, the re-suspension liquid
Are as follows: PBS, 0.5% (W/V) S9, pH7.4；
5. the preparation of test strips:
(1) capsaicine-BSA conjugate for being 1.5mg/mL according to 1 μ L/cm spraying concentration is sprayed as detection zone according to 1 μ L/cm
Applying concentration is the sheep anti-mouse igg of 0.2mg/ml as quality control region, is placed in room temperature and dries, spare；
(2) sample pad, chromatographic film, blotting paper are successively overlapped and is pasted on the bottom plate of PVC material, the detection zone of chromatographic film is close
Sample pad, quality control region cut into the immuno-chromatographic test paper strip of 4mm wide close to blotting paper detection line；
6. being stored at room temperature 10min in the magnetic bead to suspend in immuno-chromatographic test paper strip inserting step 4；
7. interpretation result:
A, with the naked eye direct interpretation: (1) detection zone signal is better than quality control region signal, illustrates without containing capsaicine or capsaicine
Content is lower than detection limit；(2) detection zone signal is equal to or is weaker than quality control region signal, illustrates to contain capsicum alkali composition in sample；
Alternatively,
B, be scanned detection to test strips with magnetic signal detector: (1) detection zone signal is better than quality control region signal, illustrates not
Content containing capsaicine or capsaicine is lower than detection limit；(2) detection zone signal is equal to or is weaker than quality control region signal, illustrates sample
Contain capsicum alkali composition in product.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811504297.XA CN109991412B (en) | 2018-12-10 | 2018-12-10 | A kind of method of capsicum alkali composition in detection grease |
| PCT/CN2018/120331 WO2020118526A1 (en) | 2018-12-10 | 2018-12-11 | Method for detecting capsaicin in oil |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811504297.XA CN109991412B (en) | 2018-12-10 | 2018-12-10 | A kind of method of capsicum alkali composition in detection grease |
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| CN110819685A (en) * | 2019-10-24 | 2020-02-21 | 广东环凯生物科技有限公司 | Listeria monocytogenes immunomagnetic bead washing liquid |
| CN113219169B (en) * | 2021-05-22 | 2021-12-17 | 北京金诺百泰生物技术有限公司 | Sealant for biological detection, preparation method of sealant, coated plate and kit using coated plate |
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| CN101354938B (en) * | 2007-07-25 | 2012-04-04 | 北京海达丰科技有限公司 | Magnetic particle for separation as well as preparation method and application thereof |
| CN101439872B (en) * | 2008-12-09 | 2011-01-05 | 彩虹集团公司 | Preparation of nano TiO2 aqueous slurry |
| CN106084059B (en) * | 2016-05-26 | 2019-10-25 | 中国农业科学院油料作物研究所 | The general specific antibody of anti-Capsaicinoids, test strips and kitchen waste grease immunochromatography method for quick identification |
| CN107144558B (en) * | 2017-05-12 | 2022-01-18 | 吉林大学 | Method for identifying illegal cooking oil by using Raman spectrum technology |
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