CN109988710B - Kit for detecting macrolide drug-resistant genes of bacteria - Google Patents
Kit for detecting macrolide drug-resistant genes of bacteria Download PDFInfo
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- CN109988710B CN109988710B CN201910383705.9A CN201910383705A CN109988710B CN 109988710 B CN109988710 B CN 109988710B CN 201910383705 A CN201910383705 A CN 201910383705A CN 109988710 B CN109988710 B CN 109988710B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
Abstract
The invention discloses a kit for detecting macrolide drug-resistant genes of bacteria, which structurally comprises a detection kit, a protective bottom sleeve, a display screen, an inner movable box cover, a handle and an electric controller, wherein the detection kit consists of a box body, a reagent bearing device, a vibration seat, a temperature controller and a drug-resistant gene detector, the time of confronting macrolide drugs and antibiotic drug-resistant strains in a reagent bottle can be rapidly obtained by utilizing a shaking plate in the inner movable box cover to be matched with the drug-resistant gene detector to shake the macrolide drugs and the antibiotic drug-resistant strains in the reagent bottle, the reagent bottle can stably and regularly move without being damaged when shaking, the macrolide drugs and the antibiotic drug-resistant strains in the reagent bottle can be vertically crossed by utilizing the vibration seat, and the macrolide drugs and the antibiotic drug-resistant strains in the reagent bottle can be obtained in different environments, the design can more accurately obtain the detected drug resistance gene data.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a kit for detecting a macrolide drug resistance gene of bacteria.
Background
After the introduction of western medicine into china, due to the clinical unreasonable application of a large amount of antibiotics, the drug resistance of bacteria related to clinical diseases is increasing, causing the imbalance of normal flora and the appearance of a large amount of drug-resistant strains. Resistant bacteria are able to withstand attack by antibiotic drugs, so that standard treatments lose effectiveness, and infections persist and can infect others. Antibiotic resistance is caused by the improper use of antibiotic drugs, particularly antibiotic drugs, and occurs when bacteria mutate or acquire resistance genes. The widespread use of macrolide drugs in the clinic has led to an increasing number of strains that are resistant to this class of antibiotics, resulting in failure of clinical therapy. There is a need for a convenient and fast method for detecting drug resistance of bacteria, so as to find drug-resistant bacteria as soon as possible, change the medication scheme, and guide clinical treatment, but macrolide drugs are stored in reagent bottles in the process of resisting antibiotic-resistant strains and are always in a static state, so that the detected data are not accurate enough, and thus, a method capable of placing macrolide drugs in various environments in the process of resisting antibiotic-resistant strains is needed to increase the accuracy of the detected data.
Disclosure of Invention
Aiming at the defects of the prior art, the invention is realized by the following technical scheme: a kit for detecting macrolide drug-resistant genes of bacteria structurally comprises a detection kit, a protective bottom sleeve, a display screen, an internal movable box cover, a handle and an electric controller, wherein the protective bottom sleeve is installed at the bottom end of the detection kit, the display screen is arranged at the front end of the detection kit, the detection kit is connected with the display screen, the internal movable box cover is arranged at the top end of the detection kit, the detection kit is movably connected with the internal movable box cover, the handles are respectively arranged at the left end and the right end of the detection kit, the detection kit and the handles are in clearance fit, the electric controller is arranged at the right end of the detection kit, and the detection kit is matched with the electric controller;
the detection kit constitute by box body, reagent acceptor, vibration seat, temperature controller, resistant medicine gene detector, the inside bottom of box body install the vibration seat, the box body inner wall on be equipped with the reagent acceptor, box body and reagent acceptor swing joint, box body front end inner wall on be equipped with the temperature controller, box body and temperature controller electricity connect, reagent acceptor top be equipped with resistant medicine gene detector, box body and resistant medicine gene detector adopt clearance fit.
As the further optimization of this technical scheme, reagent carrier constitute by mount, reagent bottle slot, slip ring, solid fixed ring, closed ring, the mount on the surface be equipped with nine reagent bottle slots to install the slip ring on six reagent bottle slots, reagent bottle slot bottom under still be equipped with closed ring, reagent bottle slot and closed ring structure as an organic whole to install solid fixed ring in the three reagent bottle slot.
As a further optimization of the technical scheme, the sliding ring further comprises a sliding ball, the sliding ball is arranged on the outer side of the sliding ring, and the sliding ring is in sliding fit with the sliding ball.
As a further optimization of the technical scheme, the closed ring further comprises a sealing ring strip, the sealing ring strip is arranged on the inner side of the closed ring, and the closed ring is connected with the sealing ring strip through glue.
As a further optimization of the technical scheme, the vibration seat is composed of a seat body, semicircular grooves and semicircular convex balls, three semicircular convex balls are arranged at the bottom end inside the seat body at equal intervals, the seat body is fixedly connected with the semicircular convex balls, and three semicircular grooves are arranged at the bottom end inside the seat body at equal intervals.
As a further optimization of the technical scheme, the inner movable box cover is further provided with a servo motor, a shaking plate, clamping grooves, rocking rods, an inner gear ring and a driving gear, the driving gear is arranged at the bottom end of the servo motor, the servo motor and the driving gear are mechanically connected through a motor shaft, the inner gear ring is arranged in the middle of the inner part of the shaking plate and is meshed with the driving gear, six clamping grooves are distributed on the surface of the shaking plate at equal intervals, and the rocking rods are arranged on four end angles of the shaking plate.
As a further optimization of the technical scheme, the fixing frame, the drug resistance gene detector and the shaking plate are parallel to each other.
As the further optimization of the technical scheme, the sealing ring strip is made of rubber materials and has good flexibility.
As a further optimization of the technical scheme, the drug resistance gene detector is connected with the shaking plate through a clamping groove.
As a further optimization of the technical scheme, the rocking plate is connected with the inner movable box cover through a rocker.
Advantageous effects
The invention relates to a kit for detecting macrolide drug-resistant genes of bacteria, after a reagent bottle is inserted into a reagent receiver, a servo motor is controlled by an electric controller to work, the servo motor drives a driving gear to rotate through a motor shaft, the driving gear is matched with an inner gear ring to drive a shaking plate to shake under an inner movable box cover, the shaking plate can shake under the condition of balance and stability by using a rocker, the shaking plate is inserted into the reagent bottle by using a drug-resistant gene detector to carry out detection and shaking, so that macrolide drugs in the reagent bottle can obtain more data in the confrontation with antibiotic-resistant strains, when the shaking plate drives the reagent bottle to shake on the sliding ring through the drug-resistant gene detector, the sliding ring shakes the reagent bottle by using a sliding ball, the macrolide drugs in the reagent bottle and the antibiotic-resistant strains are mixed and fused together, and a semicircular groove in a seat body is matched with the semicircular convex ball, the macrolide medicines and the antibiotic-resistant strains in the reagent bottles can be vertically combined, so that the macrolide medicines and the antibiotic-resistant strains can be distinguished in confrontation in different environments.
Compared with the prior art, the invention has the beneficial effects that: utilize the interior board of rocking of moving in the lid to cooperate the resistant gene detector of medicine to rock the time of macrolide class medicine in the reagent bottle and antibiotic resistant bacterial strain in antagonism can be more quick to obtain, let the reagent bottle can stable balance and regular motion when rocking through reagent acceptor, and can not let it receive the damage, reuse vibration seat makes macrolide class medicine in the reagent bottle and antibiotic resistant bacterial strain can be handed over from top to bottom, let it can obtain in the environment of difference that macrolide class medicine and antibiotic resistant bacterial strain have what difference in the antagonism, this design can be more accurate the resistant gene data who reachs the detection.
Drawings
Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:
FIG. 1 is a schematic structural diagram of a kit for detecting a bacterial macrolide drug-resistance gene of the invention.
FIG. 2 is a schematic cross-sectional view of the kit for detecting macrolide drug-resistance genes in bacteria according to the present invention.
FIG. 3 is a schematic view of the structure of the reagent vessel of the present invention.
FIG. 4 is an enlarged view of A in FIG. 2 according to the present invention.
Fig. 5 is a schematic structural diagram of the vibration seat of the present invention.
Fig. 6 is a schematic structural view of the inner movable box cover of the present invention.
In the figure: the kit comprises a detection kit-1, a protective bottom sleeve-2, a display screen-3, an internal movable box cover-4, a handle-5, an electric controller-6, a box body-1 a, a reagent receiver-1 b, a vibration seat-1 c, a temperature controller-1 d, a drug-resistant gene detector-1 e, a fixed frame-1 b1, a reagent bottle slot-1 b2, a sliding ring-1 b3, a fixed ring-1 b4, a closed ring-1 b5, a sliding bead-1 b31, a sealing ring strip-1 b51, a seat body-1 c1, a semicircular groove-1 c2, a semicircular convex ball-1 c3, a servo motor-1 f1, a shaking plate-1 f2, a clamping groove-1 f3, a rocker-1 f4, an internal gear ring-1 f5 and a driving gear-1 f 6.
Detailed Description
In order to make the technical means, the characteristics, the achievement purposes and the effects of the invention easy to understand, the preferred embodiment of the invention is further explained by combining the detailed description and the attached drawings.
Examples
Referring to fig. 1-6, the invention provides a kit for detecting macrolide drug resistance genes of bacteria, which structurally comprises a detection kit 1, a protective bottom sleeve 2, a display screen 3, an internal movable box cover 4, a handle 5 and an electric controller 6, wherein the protective bottom sleeve 2 is arranged at the bottom end of the detection kit 1, the display screen 3 is arranged at the front end of the detection kit 1, the detection kit 1 is connected with the display screen 3, the internal movable box cover 4 is arranged at the top end of the detection kit 1, the detection kit 1 is movably connected with the internal movable box cover 4, the handle 5 is arranged at each of the left end and the right end of the detection kit 1, the detection kit 1 and the handle 5 are in clearance fit, the electric controller 6 is arranged below the right end of the detection kit 1, the detection kit 1 is matched with the electric controller 6, and the detection kit 1 consists of a box body 1a, a plurality of electric controllers are arranged on the left end and right end of the detection kit 1, and the electric controller 6 are matched with each other, Reagent carrier 1b, vibration seat 1c, temperature controller 1d, resistant drug gene detector 1e constitute, the inside bottom of box body 1a install vibration seat 1c, box body 1a inner wall on be equipped with reagent carrier 1b, box body 1a and reagent carrier 1b swing joint, box body 1a front end inner wall on be equipped with temperature controller 1d, box body 1a and temperature controller 1d electricity connect, reagent carrier 1b top be equipped with resistant drug gene detector 1e, box body 1a and resistant drug gene detector 1e adopt clearance fit.
The reagent adaptor 1b comprises a fixing frame 1b1, reagent bottle slots 1b2, sliding rings 1b3, fixing rings 1b4 and a closed ring 1b5, nine reagent bottle slots 1b2 are arranged on the surface of the fixing frame 1b1, the sliding rings 1b3 are arranged on six reagent bottle slots 1b2, the closed ring 1b5 is further arranged below the bottom end of each reagent bottle slot 1b2, the reagent bottle slots 1b2 and the closed rings 1b5 are of an integrated structure, and the fixing rings 1b4 are arranged in the three reagent bottle slots 1b2,
the fixing ring 1b4 is a fixed part, which makes the reagent bottle obtain the detection data under the condition of keeping the reagent bottle in the reagent box.
The sliding ring 1b3 further comprises a sliding ball 1b31, the sliding ball 1b31 is arranged on the outer side of the sliding ring 1b3, the sliding ring 1b3 is in sliding fit with the sliding ball 1b31,
the sliding ball 1b31 is used to match the sliding ring 1b3 to slide more regularly and protect it from damage when violent shaking occurs.
The closed ring 1b5 further comprises a closed ring strip 1b51, the closed ring strip 1b51 is arranged on the inner side of the closed ring 1b5, the closed ring 1b5 is connected with the closed ring strip 1b51 by glue,
the above-mentioned sealing ring strip 1b51 is used to protect the reagent bottle from damage during operation.
The inner movable box cover 4 is further provided with a servo motor 1f1, a shaking plate 1f2, clamping grooves 1f3, a rocker 1f4, an inner gear ring 1f5 and a drive gear 1f6, the bottom end of the servo motor 1f1 is provided with the drive gear 1f6, the servo motor 1f1 and the drive gear 1f6 are mechanically connected through a motor shaft, the middle position in the shaking plate 1f2 is provided with an inner gear ring 1f5, the inner gear ring 1f5 is meshed with the drive gear 1f6, the surface of the shaking plate 1f2 is equidistantly provided with six clamping grooves 1f3, four end corners of the shaking plate 1f2 are provided with the rockers 1f4,
the rocking bar 1f4 is a rocking bar which can be matched with the rocking plate 1f2 to balance and stabilize the rocking under the inner box cover 4.
The fixing frame 1b1, the drug-resistant gene detector 1e and the shaking plate 1f2 are parallel to each other, the sealing ring strip 1b51 is made of rubber and has good flexibility, the drug-resistant gene detector 1e is connected with the shaking plate 1f2 through the clamping groove 1f3, and the shaking plate 1f2 is connected with the inner box cover 4 through the rocker 1f 4.
The principle of the invention is as follows: when the reagent bottle is inserted into the reagent receiver 1b, the electric controller 6 controls the servo motor 1f1 to work, the servo motor 1f1 drives the driving gear 1f6 to rotate through the motor shaft, the driving gear 1f6 is matched with the inner gear ring 1f5 to drive the shaking plate 1f2 to shake under the inner box cover 4, the shaking plate 1f2 uses the rocker 1f4 to shake under the condition of balance and stability, the shaking plate 1f2 uses the drug-resistant gene detector 1e to insert into the reagent bottle for detection and shaking, so that the macrolide medicine in the reagent bottle can obtain more data in confronting with the antibiotic-resistant strain, when the shaking plate 1f2 drives the reagent bottle to shake on the sliding ring 1b3 through the drug-resistant gene detector 1e, the sliding ring 1b3 uses the sliding bead 1b31 to shake the reagent bottle, and the macrolide medicine in the reagent bottle is mixed and melted with the antibiotic-resistant strain, and the semicircular groove 1c2 in the seat body 1c1 is matched with the semicircular convex ball 1c3, so that the macrolide medicines and the antibiotic-resistant strains in the reagent bottle can be vertically combined, and the differences between the macrolide medicines and the antibiotic-resistant strains in confrontation can be obtained in different environments.
The method for solving the problems comprises the following steps: utilize shake board 1f2 cooperation resistant gene detector 1e in interior movable box lid 4 to rock the time of macrolide class medicine and antibiotic resistant bacterial strain in the reagent bottle in antagonism can be more quick to obtain, let reagent bottle can stable balance and regular motion when rocking through reagent acceptor 1b, and can not let it receive the damage, reuse vibration seat 1c makes macrolide class medicine and antibiotic resistant bacterial strain in the reagent bottle can be up-and-down amalgamated, let it can obtain in the environment of difference that macrolide class medicine and antibiotic resistant bacterial strain have what difference in the antagonism, this design can be more accurate the resistant gene data who reachs the detection.
While there have been shown and described what are at present considered the fundamental principles of the invention, the essential features and advantages thereof, it will be understood by those skilled in the art that the present invention is not limited by the embodiments described above, which are merely illustrative of the principles of the invention, but rather, is capable of numerous changes and modifications in various forms without departing from the spirit or essential characteristics thereof, and it is intended that the invention be limited not by the foregoing descriptions, but rather by the appended claims and their equivalents.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (1)
1. A kit for detecting macrolide drug-resistant genes of bacteria structurally comprises a detection kit (1), a protective bottom sleeve (2), a display screen (3), an internal movable box cover (4), a handle (5) and an electric controller (6), wherein the protective bottom sleeve (2) is installed at the bottom end of the detection kit (1), the display screen (3) is arranged at the front end of the detection kit (1), the detection kit (1) is connected with the display screen (3), the internal movable box cover (4) is arranged at the top end of the detection kit (1), the detection kit (1) is movably connected with the internal movable box cover (4), the handle (5) is arranged at each of the left end and the right end of the detection kit (1), the detection kit (1) and the handle (5) are in clearance fit, the electric controller (6) is arranged at the right end of the detection kit (1), the detection kit (1) is matched with the electric controller (6), and is characterized in that: detection kit (1) constitute by box body (1 a), reagent acceptor (1 b), vibration seat (1 c), temperature controller (1 d), resistant gene detector (1 e), the inside bottom of box body (1 a) install vibration seat (1 c), box body (1 a) inner wall on be equipped with reagent acceptor (1 b), box body (1 a) and reagent acceptor (1 b) swing joint, box body (1 a) front end inner wall on be equipped with temperature controller (1 d), box body (1 a) and temperature controller (1 d) electricity connect, reagent acceptor (1 b) top be equipped with resistant gene detector (1 e), box body (1 a) and resistant gene detector (1 e) adopt clearance fit, reagent acceptor (1 b) by mount (1 b 1), reagent bottle (1 b 2), Sliding ring (1 b 3), solid fixed ring (1 b 4), closed ring (1 b 5) constitute, mount (1 b 1) be equipped with nine reagent bottle slot (1 b 2) on the surface to install sliding ring (1 b 3) on six reagent bottle slot (1 b 2), reagent bottle slot (1 b 2) bottom under still be equipped with closed ring (1 b 5), reagent bottle slot (1 b 2) and closed ring (1 b 5) be the integrated structure, and install solid fixed ring (1 b 4) in three reagent bottle slot (1 b 2), sliding ring (1 b 3) still include sliding bead (1 b 31), sliding ring (1 b 3) outside on be equipped with sliding bead (1 b 31), sliding ring (1 b 3) and sliding bead (1 b 31) sliding fit, closed ring (1 b 5) still include driving fit ring strip (1 b 51) on be equipped with closed ring strip (5 b 51), the closed ring (1 b 5) is connected with the sealing ring strip (1 b 51) through glue, the vibration seat (1 c) consists of a seat body (1 c 1), semicircular grooves (1 c 2) and semicircular convex balls (1 c 3), three semicircular convex balls (1 c 3) are distributed at the bottom end inside the seat body (1 c 1) at equal intervals, the seat body (1 c 1) is fixedly connected with the semicircular convex balls (1 c 3), three semicircular grooves (1 c 2) are distributed at the bottom end inside the seat body (1 c 1) at equal intervals, the inner movable box cover (4) is further provided with a servo motor (1 f 1), a shaking plate (1 f 2), a clamping groove (1 f 3), a rocking bar (1 f 4), an inner gear ring (1 f 5) and a driving gear (1 f 6), the bottom end of the servo motor (1 f 1) is provided with a driving gear (1 f 6), and the servo motor (1 f 2) is connected with a motor shaft (8269556), an inner gear ring (1 f 5) is arranged in the middle of the interior of the shaking plate (1 f 2), the inner gear ring (1 f 5) is meshed with the driving gear (1 f 6), six clamping grooves (1 f 3) are distributed on the surface of the shaking plate (1 f 2) at equal intervals, and rockers (1 f 4) are mounted at four end angles of the shaking plate (1 f 2); after the reagent bottle is inserted into the reagent receiver (1 b), the servo motor (1 f 1) is controlled to work through the electric controller (6), the servo motor (1 f 1) drives the driving gear (1 f 6) to rotate through a motor shaft, the driving gear (1 f 6) is matched with the inner gear ring (1 f 5) to drive the shaking plate (1 f 2) to shake under the inner box cover (4), the shaking plate (1 f 2) can shake under the condition of balance and stability through the rocker (1 f 4), the shaking plate (1 f 2) is inserted into the reagent bottle through the drug-resistant gene detector (1 e) to detect and shake, so that macrolide medicines in the reagent bottle can obtain more data in the confrontation with antibiotic-resistant strains, when the shaking plate (1 f 2) drives the reagent bottle to shake on the sliding ring (1 b 3) through the drug-resistant gene detector (1 e), the sliding ring (1 b 3) shakes the reagent bottle through the sliding bead (1 b 31), the macrolide medicines and the antibiotic-resistant strains in the reagent bottle are mixed and blended together, and the macrolide medicines and the antibiotic-resistant strains in the reagent bottle can be blended up and down by utilizing the matching of the semicircular groove (1 c 2) and the semicircular convex ball (1 c 3) in the seat body (1 c 1).
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CN110237871A (en) * | 2019-07-11 | 2019-09-17 | 武汉中浩生物技术有限公司 | A kind of stomach Function detection luminescence immunoassay kit |
CN112725172B (en) * | 2021-02-06 | 2023-08-15 | 浙江省人民医院 | Kit for detecting bacterial macrolide drug-resistant genes |
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