CN1099800A - Fungus resistant plants - Google Patents
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Abstract
To obtain a Lactuca sativa lettuce plant which exhibits resistance to Bremia lactucae by selecting a non-Dm gene intervened Bremia lactucae resistant plant derived from the origin different from the origin of Lactuca sativa, and the method of produceing the same.
Description
The present invention relates to have cultivation lettuce [be Lactuca sativa(cultivation the lettuce)] plant of the mould resistance of lettuce dish stalk.
Fungi, lettuce dish stalk is mould to be the virulence factor of " oidium " disease, this disease is all attacked large batch of lettuce crop every year.It is many year that the lettuce breeders have attempted, and attempts to breed out the lettuce that this fungi is shown resistance, and only divide successfully will importing to aspect the lettuce plant important on the agronomy obtaining section to the mould whole body resistance of lettuce dish stalk.
Mould characteristics of lettuce dish stalk are that it can produce new bacterial strain in the short relatively timed interval.New lettuce dish stalk trichoderma strain just constantly emerges in large numbers.Recently, known at least have 16 lettuce dishes that are called the NL kind to obstruct mould (36
Beschrijvende Rassenlijst voor Groentegewassen voor de Teelt onder Glas(1992) Eds.Aalbersberg IJ and Stolk J H, Centrum voor Plantenverdelingsen Reproductieonderzoek has differentiated Wageningen) and also that the lettuce dish stalk of other kinds is mould people (1992) Euphytica 61:203-211 such as () Bonnier F J M.Known demonstrating has the commodity varieties of lettuce of resistance to comprise resistance (Dm) gene of dominance usually in its genome to some lettuce dish stalk trichoderma strain, it is believed that gene wherein works by the system of gene to gene, Dm gene in this system or several Dm gene consistent with the dominance nontoxic gene of pathogenic bacteria (Bonnier, above).Dm is the abbreviation of oidium; Capital D represents that this gene is a dominance.Short of proof resistance factor is produced by an individual gene, just its feature is remembered as R.Determined Dm gene (R18 factor exception) corresponding to most of R factors.The Dm gene term of Shi Yonging also comprises such R factor in this article.
The updated and revised edition of the detection lettuce TG/13/4(1992 that describes in the UPOV rule) and the updated and revised edition in TG/13/7(1993 October 26) the regulation of specificity, homogeneity and stability be meant useful Dm gene and be meant and be suitable for measuring the test that the Dm gene exists in the lettuce.The useful Dm gene of enumerating among the UPOV is Dm2, Dm3, Dm4, Dm5/8, Dm6, Dm7, Dm10, Dm11, Dm16 and comprises the R18 factor.The UPOV statement has only the test of these genes to carry out on the ordinary method basis, and the existence of detection Dm1, Dm4, Dm15 and Dm10 needs special test.
Above Dm gene of importing does not produce and demonstrates the mould lettuce type that resistance whole body and that continue is arranged of lettuce dish stalk in the lettuce plant genome.Containing one or morely can provide lettuce plant to the Dm gene of specific lettuce dish stalk trichoderma strain resistance to demonstrate other lettuce dish stalk trichoderma strains are had very little or do not have resistance.
The mould resistance of lettuce in important lettuce type dish stalk (the mould resistance of dish stalk) lacks persistence at the appointed time on agronomy so far.
For all these reasons, breeders have found the dish in another kind of source to obstruct mould resistance.This resistance based on hereditary mechanism and do not rely on the hereditary mechanism (hereinafter such resistance being called " resistance of non--Dm gene mediated ") of the resistance of Dm gene mediated.
At a wild varieties of lettuce Lactuca saligna(like the willow lettuce) in identify existing many years (Gustafsson I.(1989) Euphytica 40: 227-232 in resistance source of non--Dm gene mediated; People (1976) Hortscience 11(6 such as Netzer D.): 612-613).Known non--Dm gene resistance at least at some like resist all known lettuces dish stalk trichoderma strains in the willow lettuce plant type effectively, especially anti-all bacterial strains (referring to UPOV, above) that can control by the useful Dm gene and the R18 factor.
In this area, also the dish of non--Dm gene mediated is obstructed mould resistance and be referred to as " horizontal resistance " and " immunity ", refer to that promptly it is independent of the resistance mechanism of Dm gene mediated and works.Can draw from top description, it is unusual ideal that the resistance of non--Dm group mediation is transferred to commercial acceptable promptly the cultivation in the lettuce plant.The previous dish of never reporting this form obstructs mould resistance and is successfully transferred in the cultivation lettuce.Caused producing the phenotype of bad plant habit and reproductivity plant is arranged (de Vries I.M.(1990) Pl.Syst.Evol.171:233-248 with the cultivation lettuce with like the trial of willow lettuce hybridization).
The difficulty that the breeder ran into causes by various factors, and the dish that comprises the characteristic of resistant gene and will derive from non--Dm gene mediated of the dissimilar cultivation lettuce that the tool dish obstructs mould resistance (as the wild-type lettuce for example like the willow lettuce) obstructs mould resistance and successfully transfers to cultivable and be the difficulty in (with commercial) acceptable lettuce on the agronomy therefrom.The dish of non-really-Dm gene mediated obstructs malicious resistance (as obstructing mould resistance by the dish like the non--Dm gene mediated that exists in the willow lettuce) and has been considered to recessive characteristic.Moreover, for obstructing mould resistance, the dish with non--Dm gene mediated breeds among the L.cativa, and at first must make the plant that is applicable to the procedure of breeding is consistent with commercial acceptable important lettuce plant on the agronomy with the cultivation lettuce fully.
Found now by the mould resistant plant of lettuce dish stalk on the F2 level, selecting to be fit to can with the cultivation lettuce not homologous non--dish of Dm gene mediated obstructs mould resistance and successfully transfers in the cultivation lettuce plant.As if moreover these phenomenons show that having the plant of resistance of non--Dm gene mediated and plant in F2 generation lacks between the growth vigor (this vigor be to use backcross between the F2 plant that filters out and the range of goods necessary to recover the plant vigor) and be related.There is being separation in F2 colony at least aspect the characteristic of the mould resistance of lettuce dish stalk.Lettuce strain important on lettuce plant of selecting can the F2 with the resistance of-Dm gene mediated non-from comprising and the agronomy is hybridized, and hybridizes product then and can be used for further breeding.
The present invention relates to cultivate lettuce plant, this plant is compared with the cultivation lettuce plant that only has based on the mould resistance of anti-lettuce dish stalk of the resistance of Dm gene mediated, can provide to have wideer responsiveness and more stable to the mould resistance of lettuce dish stalk.
The invention provides the cultivation lettuce plant (cultivation lettuce plant of the present invention) with the mould resistance capacity of anti-lettuce dish stalk, the mould resistance of anti-lettuce dish stalk of this plant comprises the resistance of non--Dm gene mediated.
The resistance of non--Dm gene mediated preferably derives from like the willow lettuce.Suitable is that cultivation lettuce plant of the present invention can educate fully.
Cultivation lettuce plant of the present invention can comprise one or more Dm genes or not comprise it in its genome.
Cultivation lettuce plant of the present invention is that the L.sativa plant is the lettuce plant that is suitable for eating.All seeds of cultivation lettuce plant of the present invention can both produce the cultivation lettuce plant that shows the resistance of the mould non--Dm gene mediated of lettuce dish stalk in essence.The phenotype feature of described plant be enough uniformly so that for commerce acceptance and meet the standard that commercial competent authority proposes.
Cultivation lettuce plant of the present invention can be tetraploid (2n=2x=36) or diploid (being 2n=2x=18) in essence, but diploid preferably.Described herein term " plant " comprises plant part, cell and seed.
The example of plant part comprises the part (lettuce heads and Qi Ye) that is applicable to consumption and is suitable for the part that plant produces, for example organ pipe tissue (for example leaf, stem, root, bud etc.), protoplastis, somatic embryo, flower pesticide, stamen, petiole and culturing cell etc.Utilize plant tissue culture technique also can or breed cultivation lettuce plant of the present invention from the plant part cultivation.These technology are known in the art.
When being subjected to the trichoderma strain invasion and attack of lettuce dish stalk and/or infecting, cultivation lettuce plant of the present invention is compared with the lettuce plant of the resistance that has a Dm gene or several Dm gene mediateds itself, can show the resistance of the lettuce dish stalk trichoderma strain of anti-more wide region.Therefore, under test conditions, can observe the resistance of non--Dm gene mediated, and under normal field or greenhouse experiment, also can observe this resistance.
Cultivation lettuce plant of the present invention can be obtained by following method
ⅰ) carry out initial hybridization, one of them parent's genetic material is to coil the wild-type lettuce plant that obstructs mould resistance by the lettuce that shows non--Dm gene mediated in cotyledon and true leaf to provide, another parent's genetic material is by the mould nonresistant cultivation lettuce plant of lettuce dish stalk is provided, next carry out self-pollination and produce F2 generation, be chosen in the plant (R degeneration plant) that cotyledon period and leaf period have anti-lettuce dish stalk trichoderma strain resistance; Then
ⅱ) a) R is degenerated plant with have required phenotypic cultivation lettuce plant and hybridize;
B) self-pollination is to produce F2 generation at least;
C) the R degeneration plant of selection generation: and
D) if desired, repeating step is a) to c), up to the lettuce plant that obtains to adapt with cultivation lettuce plant; And
ⅲ) e) the R degeneration plant that will from the lettuce plant that adapts, select with show required phenotypic different cultivation lettuce plant and hybridize;
F) self-pollination is to produce F2 generation at least;
G) the R degeneration plant of selection generation, and
H) if desired, repeating step e) to g) so that obtain cultivation lettuce plant, and
ⅳ) with ⅲ) in the plant self-pollination that obtains to obtain F4 generation at least.
Wherein any stage in the described method of hybridizing, stop female parent's self-pollination of desire hybridization.
Usually, the breeding method that obtains plant of the present invention comprises and differentiates a kind of suitable wild-type lettuce plant that shows the mould resistance of anti-lettuce dish stalk at cotyledon period and leaf period.Preferably, wild-type lettuce plant is a kind of seemingly willow lettuce plant of carrying the mould resistance of anti-lettuce dish stalk of non--Dm gene mediated.This conclusion is for example passed through, cultivate wild-type plant colony and coil the mixture that obstructs trichoderma strain and infect these plant with lettuce, perhaps these plant are contacted for some time in the cotyledon etap with each lettuce dish stalk trichoderma strain, infect again with lettuce dish stalk is mould at leaf period then, and select those plant that all show resistance two stages.If do not show the visible sporulation on the leaf then proved resistance.If use such step to be called the R degradation experiment in the procedure of breeding, the plant that promptly shows the mould resistance of anti-lettuce dish stalk is that R degenerates.The wild-type lettuce plant strain growth that all shows resistance two leaf stages that to select then be ripe plant and with the mould nonresistant cultivation lettuce plant of lettuce dish stalk is hybridized.The cultivation lettuce plant type that is applicable to initial hybridization is fit to be selected from the cultivation lettuce plant that shows required agronomy feature.Select all plant types all can be based on its phenotype.By hybridizing plant generation seed simply, collect F1 seed and sowing to produce autophilous subsequently plant, make F2 for the filial generation that can obtain initial hybridization.
In another kind of optional method, can use embryoid redemption technology, wherein can from the hybridization plant in embryoid stage, gather in the crops initial hybridization product and in suitable culture medium, its cultivation be formed root and bud up to.In case formed enough roots and bud, the plantlet that obtains can be transferred in the soil and under suitable greenhouse experiment then and be grown, set up a plant group up to, therefrom can select suitable plant then and be further used for the procedure of breeding.The scheme that is applicable to the embryoid rescue method is known in the art, Maisonneuve B. for example, Agronomie(1987), 7(5): 313-319.Make F1 produce F2 generation then as previously mentioned, and therefrom select suitable plant according to phenotype for self-pollination.By the scheme of this paper general introduction suitable F2 plant is carried out the R degradation experiment then, and with R degenerate plant with carried that the suitable cultivation lettuce plant of required feature hybridizes to be used in particular for recovering growth vigor on the agronomy, self-pollination produces F2 generation, therefrom selects suitable plant once more and carries out the R degradation experiment.Repeat this step, up to the lettuce plant that obtains to adapt with the cultivation lettuce.The plant that adapts that the thus obtained dish that contains non--Dm gene mediated can be obstructed mould resistance then is used for anyly further can cultivating the lettuce procedure of breeding as primitive variety.A kind of lettuce plant of adaptability is a kind ofly to hybridize with cultivation lettuce type easily and be that the oneself can educate.Because lettuce is the self-pollination plant, the suitable primitive variety of therefore setting up the mould resistance of lettuce dish stalk that contains non--Dm gene mediated is important.Whether show the resistance of non--Dm gene mediated in order to establish a strain that adapts, it is infected with lettuce dish stalk trichoderma strain during for wild-type lettuce cotyledon period that plant is described in and first leaf period by this paper.Therefore, preferably, the lettuce plant that provides cultivation lettuce a kind of and the mould resistance of lettuce dish stalk that in genome, comprises non--Dm gene mediated to adapt.In case obtain and cultivate the plant that lettuce adapts, repeat R degradation experiment step, and the R degeneration plant that selection is used for and cultivation lettuce plant hybridizes, and self-pollination to be producing at least F2 generation, up to the cultivation lettuce type of the agronomy appealing that obtains a gene resistance of having mixed non--Dm mediation in its genome.The plant strain of the resistance that contains non--Dm gene mediated that filters out is repeatedly carried out self-pollination up to obtaining cultivation lettuce plant.Carry out self-pollination and be in order to obtain F4 generation at least, preferably to obtain F5 generation, more preferably obtain F6 generation, it is believed that wherein a generation or in many generations, the non--resistance of Dm gene mediated is stably to be incorporated in the genome.It is believed that but such plant is suitable for producing the seed of the cultivation lettuce plant of being created in of sales volume comprise non--Dm gene mediated in its genome the mould resistance of lettuce dish stalk.
The hybridization of self-pollination plant requires to stop the plant that is used as female parent to carry out self-reproduction.By extract the male portion can bear the regeneration organ of extracing by hand with hand or spend by chemical process and/or use in water finish and remove male portion and finish above-mentioned work.Described above-mentioned emasculating method is well known in the art.
Those skilled in the art will recognize that the procedure of breeding of summarizing above will take the procedure of breeding many year and that summarize above and be suitable for various types of lettuce breedings of (comprising indoor, greenhouse, outdoor field lettuce or the like) of cultivating in this area.
Provided below and further illustrated embodiments of the invention.It is to be understood that also simultaneously that described embodiment should not regard as will limit the present invention by any way.
Embodiment 1
The inoculum of the mixture by will comprising lettuce dish stalk trichoderma strain is applied on after planting the 6th day the leaf, and when after planting 13 days and 18 days, observe the method for cotyledon and first leaf of plant Adulthood respectively, thereby detect the anti-lettuce dish of S3113 and S3114 stalk trichoderma strain N11 to N116, and the dish of other inside called after TV, ITA1 that are separated to and GER obstructs the mould resistance of lettuce dish stalk of mould fungal bacterial strain.Proof S3113 and S3114 have resistance to all lettuce dish stalk trichoderma strains that detected, and be as shown in table 1, and the resistance of wherein passing through Dm gene generation itself is at least one lettuce dish stalk trichoderma strain sensitivity.
In table 1 ,-represent resistance or partial resistance are arranged respectively with (-) to lettuce dish stalk is mould, and (+) expression is obstructed mould sensitivity to the lettuce dish.
Table 1
Important Dm gene disk stalk plum NL kind S﹠amp; The G isolate
2?-?-?-?-?-?-?- +?+?-?+?-?+?+?-?+?-?-?-?+?+?+?+?+?+
-?3?-?-?-?-?-?- -?+?-?-?+?-?+?+?+?-?-?+?+?+?+?+?+?+
-?-?5/8?-?-?-?-?- -?+?+?+?-?+?-?+?+?+?+?+?+?+?+?+?+?+
-?-?-?6?-?-?-?- -?+?+?-?-?-?+?+?+?+?+?-?+?(-)?+?+?-?+
-?-?-?-?7?-?-?- -?-?+?+?+?-?+?+?+?+?+?+?-?+?+?+?+?+
-?-?-?-?-?11?-?- -?-?-?-?-?+?-?-?-?(-)?+?+?+?+?+?-?+?+
-?-?-?-?-?-?16?- -?-?-?-?-?-?-?-?-?+?+?-?-?-?+?+?-?+
-?-?-?-?-?-?-?18 -?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-?+?+
S?3113 -?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-
S?3114 -?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-
2?-?-?-?7?-?-?- -?-?-?+?-?-?+?-?+?-?-?-?-?+?+?+?+?+
2?-?-?-?-?11?-?- -?-?-?-?-?+?-?-?-?-?-?-?+?+?+?-?+?+
-?3?-?-?7?-?-?- -?-?-?-?+?-?+?+?+?-?-?+?+?+?+?+?+?+
-?3?-?-?-?11?-?- -?-?-?-?-?-?-?-?-?-?-?+?+?+?+?-?+?+
-?-?5/8?-?7?-?-?- -?-?+?+?-?-?-?+?+?+?+?+?-?+?+?+?+?+
-?-?5/8?-?-?11?-?- -?-?-?-?-?+?-?-?-?-?(-)?+?+?+?+?+?+?+
-?-?-?6?-?11?-?- -?-?-?-?-?-?-?-?-?(-)?+?-?+?(-)?+?-?-?+
2?3?-?-?7?-?-?- -?-?-?-?-?-?+?-?+?-?-?-?-?+?+?+?+?+
2?3?-?-?-?11?-?- -?-?-?-?-?-?-?-?-?-?-?-?+?+?+?-?+?+
-?3?5/8?-?7?-?-?- -?-?-?-?-?-?-?+?+?-?-?+?+?+?+?+?+?+
-?3?-?6?-?11?-?- -?-?-?-?-?-?-?-?-?-?-?-?+?(-)?+?-?+?+
-?-?5/8?6?-?11?-?- -?-?-?-?-?-?-?-?-?-?+?-?+?(-)?+?-?-?+
2?-?-?-?-?-?16?- -?-?-?-?-?-?-?-?-?-?-?-?-?-?+?+?-?+
-?3?-?-?-?-?16?- -?-?-?-?-?-?-?-?-?-?-?-?-?-?+?+?-?+
-?-?-?-?-?11?16?- -?-?-?-?-?-?-?-?-?(-)?+?-?-?-?+?-?-?+
-?3?-?-?-?11?16?- -?-?-?-?-?-?-?-?-?(-)?-?-?-?-?+?-?-?+
-?-?-?-?-?-?-?18 -?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-?+?+
-?-?-?6?-?-?-?18 -?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-?+
S?3113 -?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-
S?3114 -?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-
Embodiment 2
With one lettuce dish stalk trichoderma strain, and the mixture of NL16, GER and TV lettuce dish stalk trichoderma strain detects the mould resistance of anti-lettuce dish stalk of S3113 and S3114 respectively, and compares with the resistance of the different commodity varieties of lettuce of the mould resistance of anti-lettuce dish stalk that only contains a Dm gene mediated.As from table 2 see that S3113 and S3114 have resistance to all lettuces dish stalk trichoderma strains that detect, and the lettuce type of resistance that only contains the Dm gene mediated is to some lettuce dish stalk trichoderma strain sensitivity.
Table 2
Kind Dm gene NL16 Ita 1 TV, NL16 and
The mixture of GER
Dandie Dm?3 + + +
UCDm?2 Dm?2 + + +
Judy Dm?3,Dm?11 + + +
Ramona Dm?3,Dm?11 + + +
Safier Dm?3+Dm?7+Dm?16 + - +
Capitan Dm?11 + + +
The Mariska R factor 18-++
S3113 - - -
S3114 - - -
Inoculum: NL 16, Ita 1, the mixture of NL 16+GER+TV
Infect at cotyledon period: after planting 0+6 days
Observe cotyledon: after planting 0+13 days
Observe leaf: after planting 0+18 days
-: resistance
+: sensitivity
On May 19th, 1993 S3113 and S3114 are stored in Scottish international preservation mechanism, country's collection place (National Collections of Industrial and Marine Bacteria Ltd of industry and marine bacteria company limited, International Depository Authority, Scotland), and respectively giving preserving number is NCIMB40556 and NCIMB40557.
Breeding plan
Breeding plan 1: an embodiment who at first the mould resistance of lettuce dish stalk of non--Dm gene mediated is imported to the procedure of breeding in the cultivation lettuce has been described in this plan.The plant S3113 that obtains can be used for further self-pollination.S3113 had both contained non--Dm gene mediated in genome dish obstructs mould resistant gene and also contains Dm gene, Dm3 and Dm11.
The female plant that produces in this procedure of breeding is on the left side of figure, and male plant is on the right side of figure.To be labeled as " B28 " like the willow lettuce, a kind of in-house name, the result shows that this plant contains a kind of whole body resistance to the mould non--Dm gene mediated of lettuce dish stalk through identifying, B28-60 is the in-house name of naming lettuce strain important on first agronomy, in this strain, imported the mould resistance of lettuce dish stalk of non--Dm gene mediated, and this strain adapts with the cultivation lettuce (be self-adaptation, can educate fully, hybridize with the cultivation lettuce easily), and can be used as primitive variety in all procedures of breeding subsequently.B28-60 is the F3 by F2 * Palmyran hybridization back selfing obtains.Name " Novir " is meant that first obstructs the lettuce that trichoderma strain does not have resistance from what INRA obtained to any lettuce dish.Other name " Palmyran ", " Jessy ", " Ramona ", " Judy ", " Thirana " and " Penny " are meant the S﹠amp that contains Dm gene, Dm3 and Dm11 in its genome; G Seeds BV range of goods." E-207 ", " E-99 " are meant the S﹠amp of in-house preparation; G seed-test strain.Ramsal-19 is that another can be grown under the condition of short period of time, and contains the in-house name of lettuce strain important on the agronomy of B28 resistance.Ramsal-19 is used as the original system of all greenhouse lettuce types, and is the F3 by F2 * Ramona hybridization back selfing obtains.Above-mentioned all kinds of listing with from being to contain the plant hybridization that the F2 of the resistance of the mould non--Dm gene mediated of anti-lettuce dish stalk selects at least.All F2 plant of using in the hybridization of whole breeding plan and/or selfing all are the R degeneration plant that filters out.With F2 * Thirana, and F4 * Judy hybridization is so that import in commodity phenotype commercially attractive, through improving, and by means of the early stage B28 resistance that imports, this commodity phenotype also contains a stable B28 resistance that imports.The F6 that selfing produces is that S3113 is considered to as the kind that stably imports the B28 resistance in its genome.
Breeding plan 2: according to the breeding mode of selecting in the breeding plan 1, that different is F2 * E-207 basically, and promptly (selfing obtains) F2 plant and dissimilar " E-99 ", the F2R(that obtains from screening by hybridization also is a kind of S﹠amp; G Seeds B.V. tests kind) hybridization, the F4 and other the type of merchandise " Penny " (a kind of S﹠amp that obtain from F2R degeneration selfing; The G Seeds B.V. type of merchandise) hybridization, and selfing obtains coming from the F6 of selected F2R degeneration plant, i.e. S3114.
Breeding plan 1
Plant product based material: like willow lettuce * cultivation lettuce
Breeding plan 2
Plant product based material: like willow lettuce * cultivation lettuce
Claims (11)
1, to the mould cultivation lettuce plant that resistance is arranged of lettuce dish stalk, the mould resistance of lettuce dish stalk of the non--Dm gene mediated that it is characterized in that they comprise in its genome.
2, cultivation lettuce plant according to claim 1 is characterized in that non--Dm gene provides the resistance of anti-Holland's dish stalk mould kind of NL1-7, NL9-16, TV and ITA1.
3, cultivation lettuce plant according to claim 1 and 2, wherein non--Dm gene is from obtaining like the willow lettuce.
4, according to any one described cultivation lettuce plant among the claim 1-3, wherein cultivable lettuce plant can educate fully.
5, according to any one described cultivation lettuce plant among the claim 1-4, this plant comprises a non--Dm gene and one or more Dm gene in its genome.
6, obtain the method for the described cultivation of claim 1-5 lettuce plant, this method comprises:
ⅰ) carry out initial hybridization, one of them parent's genetic material is to coil the wild-type lettuce plant that obstructs mould resistance by the lettuce that shows non--Dm gene mediated in cotyledon and true leaf to provide, another parent's genetic material is to be provided by the cultivation lettuce plant to the mould resistance of lettuce dish stalk, next carry out self-pollination and produce F2 generation, be chosen in the plant (R degeneration plant) that cotyledon period and leaf period have anti-lettuce dish stalk trichoderma strain resistance; Then
ⅱ) a) R is degenerated plant with have required phenotypic cultivation lettuce plant and hybridize;
B) self-pollination is to produce F2 generation at least;
C) the R degeneration plant of selection generation: and
D) if desired, repeating step is a) to c), up to the lettuce plant that obtains to adapt with cultivation lettuce plant; And
ⅲ) e) the R degeneration plant that will from the lettuce plant that adapts, select with show required phenotypic different cultivation lettuce plant and hybridize;
F) self-pollination is to produce F2 generation at least;
G) the R degeneration plant of selection generation, and
H) if desired, repeating step e) to g) so that obtain cultivation lettuce plant, and
ⅳ) with ⅲ) in the plant self-pollination that obtains to obtain F4 generation at least.
Wherein any stage in the described method of hybridizing, stop female parent's self-pollination of desire hybridization.
7, method according to claim 6, wherein the male parent of initial hybridization is a kind of cultivation lettuce plant, and female parent is a kind of like willow lettuce plant.
8, according to claim 6 or 7 described methods, wherein used male parent is a cultivation lettuce plant in the hybridization, and female parent carries the mould resistance of anti-lettuce dish stalk that comes from like non--Dm gene mediated of willow lettuce.
9, the cultivation lettuce plant that obtains according to any one described method among the claim 6-8.
10, according to any one described head or leaf of cultivating the lettuce plant in claim 1-5 and 9.
11, according to any one described seed or other breeding material of cultivating the lettuce plant in claim 1-5 and 9.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP93810385.0 | 1993-05-27 | ||
| EP93810385 | 1993-05-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1099800A true CN1099800A (en) | 1995-03-08 |
Family
ID=8214975
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 94105931 Pending CN1099800A (en) | 1993-05-27 | 1994-05-26 | Fungus resistant plants |
Country Status (6)
| Country | Link |
|---|---|
| JP (1) | JPH06343361A (en) |
| CN (1) | CN1099800A (en) |
| AU (1) | AU683457B2 (en) |
| HU (1) | HU9401588D0 (en) |
| IL (1) | IL109777A0 (en) |
| ZA (1) | ZA943752B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2023712A4 (en) * | 2006-06-15 | 2009-07-08 | Progeny Advanced Genetics | Lettuce breeding method |
| EP2272328A1 (en) | 2009-07-06 | 2011-01-12 | Syngenta Participations AG | Plant resistant to a pathogen |
-
1994
- 1994-05-25 IL IL10977794A patent/IL109777A0/en unknown
- 1994-05-25 AU AU63316/94A patent/AU683457B2/en not_active Ceased
- 1994-05-26 CN CN 94105931 patent/CN1099800A/en active Pending
- 1994-05-26 JP JP6112550A patent/JPH06343361A/en active Pending
- 1994-05-26 HU HU9401588A patent/HU9401588D0/en unknown
- 1994-05-27 ZA ZA943752A patent/ZA943752B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JPH06343361A (en) | 1994-12-20 |
| IL109777A0 (en) | 1994-08-26 |
| HU9401588D0 (en) | 1994-08-29 |
| AU6331694A (en) | 1994-12-01 |
| ZA943752B (en) | 1995-11-27 |
| AU683457B2 (en) | 1997-11-13 |
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