CN109975283A - Visual indicating label of a kind of identification Meat and preparation method thereof and application - Google Patents
Visual indicating label of a kind of identification Meat and preparation method thereof and application Download PDFInfo
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- CN109975283A CN109975283A CN201910283409.1A CN201910283409A CN109975283A CN 109975283 A CN109975283 A CN 109975283A CN 201910283409 A CN201910283409 A CN 201910283409A CN 109975283 A CN109975283 A CN 109975283A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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Abstract
Application the present invention relates to a kind of visual indicating label for identifying Meat and preparation method thereof with it.The visual indicating label production method includes preparing spinning solution and preparing porous nanofiber membrane.The visual indicating label of the present invention accurately can monitor in real time Meat by naked eyes, provide a kind of practicable method for meat quality detection.
Description
[technical field]
The invention belongs to technical field of food production.More particularly it relates to a kind of for identifying Meat
Visual indicating label, further relate to the production method for identifying the visual indicating label of Meat, further relate to institute
State the application for identifying the visual indicating label of Meat.
[background technique]
Meat is because containing protein easy to digest at high proportion, high heat fat, vitamin and other micronutrients.It is all this
A little ingredients are most important for the normal operation of metabolic process.So it is allowed to become people's indispensable a part daily.
In the past decade, over time, global production of meat is significantly increased, it is contemplated that will be further increased.But
Be, when meat is under a variety of transports and storage condition, due to the high-content of fat and water, product be easiest to by microbial contamination and
Lipid oxidation, so and not all meat product can be by simple " before best " or " use date " come Precise Representation meat
Freshness.This may cause consumer and abandons the mutton for being still adapted to consumption, cause economic loss, or eat up no longer safe
Meat, cause the harm to health.The stale of meat can cause less serious case's diarrhea, and severe one is dead, so meat is new
Freshness has been related to the health and life security of people, and the harmony of the sound development and society that are also related to country is stablized.
It is estimated that China is as high as one third in the food that consumer phase is wasted,；Moreover, in developed country, it is most of all to waste
In distribution or consumer phase.This causes people to the interest for monitoring food quality intelligent packaging in real time naturally.
Meat is protein source common in human nutrition, they are also the abundant nutrition source of microorganism growth.With
Microorganism growth.Have found that mucus is formed in meat, structural constituent is degraded, peculiar smell and cosmetic variation.In microbial spoilage meat
Methylamine, dimethylamine, trimethylamine, cadaverine and putrescine are generally produced during product.Amino acid is decomposed by deamination and generates ammonia by microorganism
And decarboxylation generates biogenic amine, such as cadaverine, putrescine, spermidine, spermine etc..And cadaverine and the putrescine (Metabolic byproducts of meat corruption
Object) using the index as meat corruption.The production of these diamines carries out in the following manner:
Biogenic amine not only indicates the freshness of food, but also also adversely affects to human health and physiological function.
An obstacle for detecting biogenic amine is their not intrinsic characteristic absorptions or discharge.BA detection method can be divided into two
Class.The first kind is based on detection BA itself.Another kind is the detection based on amino acid decarboxylases active microorganism.Based on amino acid
The detection of decarboxylase microorganism is using can be according to culture medium color by pH indicator and sub- substrate composed growth medium
Variation identification BA aggregation.Detection based on biogenic amine has high performance liquid chromatography (HPLC), gas-chromatography (GC), thin-layer chromatography
(TLC), ion-exchange chromatography, biosensor and Capillary Electrophoresis (CE) are the methods for detecting BA.But it is to provide for
For UV or the pigment of fluorescence detection, these detection techniques need to reduce its polarity.So process usually requires derivatization completion.
Dansyl Cl, chlorobenzoyl chloride and o-phthalaldehyde are commonly used in derivatization.Complex steps just have destructiveness to the tissue of meat, together
When with greater need for profession personnel carry out instrument operation and data analysis.
For this reason, intelligent packaging, the optics or view of amine sensor and other food spoilages are had been observed that in recent years
The development for feeling sensor, monitors freshness in such a way that non-destructive or consumer are applicable in.The non-destructive of nearest some amine refers to
Mark includes: modified carbon nano-tube, electronic nose, RFID (radio frequency identification) label, colorimetric sensor.But these methods are being brought accurately
While also bring interpretation of result complexity, the disadvantages of taking a long time.Currently, there is some sensors to be able to detect food spoilage.
Some are technologically advanced, but are faced with highly sensitive low, complicated for operation, the problems such as material is dangerous, it is easy to operate still
The problems such as sensitivity is not high enough.This pushes to popular increase another economy and practical obstacle for its intelligent packaging.
[summary of the invention]
[technical problems to be solved]
The object of the present invention is to provide a kind of for identifying the visual indicating label of Meat.
It is a further object to provide described for identifying the production side of the visual indicating label of Meat
Method.
It is a further object to provide application of the visual indicating label in identification Meat.
[technical solution]
The present invention is achieved through the following technical solutions.
The present invention relates to a kind of for identifying the visual indicating label production method of Meat.
The step of visual indicating label production method, is as follows:
A, spinning solution is prepared
At room temperature, 0.01~10 parts by weight fluorescent reporter dyes are added in 90~110 parts by weight solvent, use magnetic
The stirring of power blender, is completely dissolved fluorescent reporter dyes, then adds 5~30 parts by weight polymer, uses magnetic stirring apparatus
Stirring, polymer is completely dissolved, and is uniformly mixed, and the spinning solution is obtained；
B, porous nanofiber membrane is prepared
Using electrostatic spinning machine 20~50 DEG C of temperature, relative humidity 20~90%, 2~40kv of spinning voltage, spinning away from
Under conditions of 8~30cm and 0.2~10.0ml/h of injection speed, the spinning solution for allowing step A to obtain carries out spinning 30-60min,
A kind of porous nanofiber membrane is obtained, the heating at temperature 60 C~100 DEG C is then placed in baking oven and removes extra solvent, obtain
To the porous nanofiber membrane；The porous nanofiber membrane is cut into the circular film of diameter 1cm, is obtained described visual
Indicating label.
A preferred embodiment of the invention, the weight ratio of fluorescent reporter dyes, polymer and solvent is 0.1~
8.0:10~25:95~105.
According to another preferred method of implementation of the present invention, the fluorescent reporter dyes are one or more to be selected from 1,2-
Dihydro-quinoxaline derivative, Rhodamine Derivatives, imidodicarbonic diamide or quantum dot fluorescent reporter dyes.
According to another preferred method of implementation of the present invention, the polymer be it is one or more selected from polylactic acid, it is poly-
Styrene, acetate fiber, polyethylene oxide, polycaprolactone or polyvinylpyrrolidone polymer.
According to another preferred method of implementation of the present invention, the solvent is one or more selected from methanol, ethyl alcohol, three
Fluoroacetic acid, acetone, chloroform, N, the solvent of N- dimethylformamide or dimethyl sulfoxide.
According to another preferred method of implementation of the present invention, the fluorescent reporter dyes are in the solvent in revolving speed
2~6h is stirred under 100~1000rpm.
According to another preferred method of implementation of the present invention, in 10~1000rpm of revolving speed after the addition polymer
Under continue 20~30h of stirring.
According to another preferred method of implementation of the present invention, the diameter of the fiber as obtained by polymer dope spinning is
100~1000nm；The thickness of the porous nanofiber membrane is 0.05~5.0mm.
It is prepared for identifying the visual indicating label of Meat that the invention further relates to the production methods.
The invention further relates to application of the visual indicating label in identification Meat.By the visual indicating label
The freshness for storing real-time monitoring meat at 4~30 DEG C of temperature together with meat, with volatile bio amine amount in packing
Increase, its color changes yellowly by red, and fluorescence is changed into hyperfluorescence from unstressed configuration, is sentenced according to color and fluorescence intensity
Break its Meat；Then, which is placed in again on the bottleneck equipped with acid solution, in the effect of acid vapors
Under, its color is transformed into original red by yellow, and fluorescence is changed into unstressed configuration from hyperfluorescence, and then it can repeat to make
With.
The present invention is described in more detail below.
The present invention relates to a kind of for identifying the visual indicating label production method of Meat.
Meat can generate biogenic amine (ammonia, cadaverine, putrescine, trimethylamine etc.) under microbial action, have proven to these lifes
Object amine can be used as the putrid and deteriorated indicator variable of meat.The present invention develop it is a kind of have to these biogenic amines it is highly sensitive honest and clean
Valence nano fibrous membrane, this nano fibrous membrane can change with gaseous biological amine shows visibly different color and fluorescence intensity pair
Instruction variation, can then allow consumer to be capable of the freshness of real-time monitoring meat.
In the present invention, Meat is it should be understood that meat degree putrid and deteriorated in storage.Described
Meat is the livestocks poultry meat such as pig, sheep, ox, chicken, duck.
In the present invention, visual indicating label is it should be understood that a kind of with highly sensitive, visualization real-time detection meat is new
The porous nanofiber membrane label of freshness.
The step of visual indicating label production method, is as follows:
A, spinning solution is prepared
At room temperature, 0.01~10 parts by weight fluorescent reporter dyes are added in 90~110 parts by weight solvent, use magnetic
The stirring of power blender, is completely dissolved fluorescent reporter dyes, then adds 5~30 parts by weight polymer, uses magnetic stirring apparatus
Stirring, polymer is completely dissolved, and is uniformly mixed, and the spinning solution is obtained；
In the present invention, main function of the fluorescent reporter dyes in visual indicating label is that real-time visual is aobvious
Show the freshness of meat.
The fluorescent reporter dyes that the present invention uses are one or more to spread out selected from 1,2- dihydro-quinoxaline derivative, rhodamine
The fluorescent reporter dyes of biology, imidodicarbonic diamide or quantum dot, they are all current market sales of products, such as by Ai Yi
1,2- dihydro-quinoxaline derivative that saliva Biotechnology Co., Ltd is sold with trade name 1,2- dihydro-quinoxaline derivative, by wide
Rhodamine Derivatives that Zhou Bo cypress trade Co., Ltd is sold with trade name Rhodamine Derivatives, by BOC Sciences company with
Trade name imidodicarbonic diamide sale imidodicarbonic diamide, by Suzhou Xing Shuo nanosecond science and technology Co., Ltd with trade name CdSe/ZnS
The quantum dot of QDs sale.
In the present invention, main function of the polymer in visual indicating label is the polymerization to form certain viscosity
Object solution is convenient for pulling into nanofibers under high-pressure electrostatic effect.
The polymer that the present invention uses is one or more selected from polylactic acid, polystyrene, acetate fiber, polycyclic oxygen second
The polymer of alkane, polycaprolactone or polyvinylpyrrolidone, they are all current market sales of products, such as by Aladdin
Acetate fiber that company is sold with trade name cellulose acetate, by Shanghai Mike woods biochemical technology Co., Ltd with trade name polycyclic
The polyethylene oxide of oxidative ethane sale is gathered in oneself by Shanghai North Connaught biological technology CO., LTD. with what trade name polycaprolactone was sold
Ester, the polyvinylpyrrolidone sold by all biological Co., Ltd of Shanghai crystalline substance with trade name polyvinylpyrrolidone.
In the present invention, main function of the solvent when making visual indicating label is dissolution polymer.
The solvent that the present invention uses is one or more selected from methanol, ethyl alcohol, trifluoroacetic acid, acetone, chloroform, N, N-
The solvent of dimethylformamide or dimethyl sulfoxide, they are all current market sales of products, such as are tried by Chinese medicines group chemistry
Trifluoroacetic acid that agent Co., Ltd is sold with trade name trifluoroacetic acid, by Sinopharm Chemical Reagent Co., Ltd. with trade name three
The chloroform of chloromethanes sale, the N sold by Sinopharm Chemical Reagent Co., Ltd. with trade name N, N- dimethylformamide,
N- dimethylformamide, the dimethyl sulfoxide sold by Sinopharm Chemical Reagent Co., Ltd. with the dimethyl sulfoxide of trade name.
According to the present invention, the fluorescent reporter dyes in the solvent using magnetic stirring apparatus revolving speed 100~
2~6h is stirred under conditions of 1000rpm, its object is to so that the fluorescent reporter dyes is dissolved completely in the solvent
In.
Meanwhile the system for adding the polymer being allowed to continue 20~30h of stirring under conditions of 10~1000rpm of revolving speed,
Its object is to be completely dissolved the polymer, the true solution of a fluorescent reporter dyes and polymer is obtained.
In the present invention, when the dosage of polymer and solvent is in the range, if the dosage of fluorescent reporter dyes
Lower than 0.01 parts by weight, then spinning the film come is white, can't see dye colour and is not available；If fluorescent reporter dyes
Dosage is higher than 10 parts by weight, then fluorescent dye excessive concentration causes that fluorescence self-quenching occurs, and reduces the using effect of indicating label；
Therefore, it is reasonable that the dosage of fluorescent reporter dyes, which is 0.01~10 parts by weight,；
Similarly, when the dosage of fluorescent reporter dyes and solvent is in the range, if the dosage of polymer is lower than 5
Parts by weight, then spinning fluid viscosity is inadequate, is unable to spinning；If the dosage of polymer is higher than 30 parts by weight, the viscosity of spinning solution
It is excessively high, it may appear that the phenomenon that Severe blockage syringe needle；Therefore, it is suitable that the dosage of polymer, which is 5~30 parts by weight,；
When the dosage of fluorescent reporter dyes and polymer is in the range, if the dosage of solvent is lower than 90 weight
Part, then polymer cannot sufficiently dissolve；If the dosage of solvent is higher than 110 parts by weight, the viscosity of spinning solution is inadequate；Therefore,
The dosage of solvent is that 90~110 parts by weight are appropriate；
Preferably, the weight ratio of fluorescent reporter dyes, polymer and solvent is 0.1~8.0:10~25:95~105.
B, porous nanofiber membrane is prepared
Using electrostatic spinning machine 20~50 DEG C of temperature, relative humidity 20~90%, 2~40kv of spinning voltage, spinning away from
Under conditions of 8~30cm and 0.2~10.0ml/h of injection speed, the spinning solution for allowing step A to obtain carries out weaving 30-60min,
A kind of porous nanofiber membrane is obtained, the heating at temperature 60 C~100 DEG C is then placed in baking oven and removes extra solvent, obtain
To the porous nanofiber membrane；The porous nanofiber membrane is cut into the circular film of diameter 1cm, is obtained described visual
Indicating label.
The electrostatic spinning machine that the present invention uses is current market sales of product, such as is worked in peace and contentment development section by Beijing Yongkang
The electrostatic spinning machine that skill Co., Ltd is sold with trade name electrostatic spinning machine.The electrostatic spinning machine is provided according to its operational manual
Operating condition (20~50 DEG C of temperature, relative humidity 20~90%, 2~40kv of spinning voltage, 8~30cm of spinning distance and note
0.2~10.0ml/h of firing rate degree) carry out spinning.
It is measured using transmission microscopy SEM4000 and is calculated by image plus pro6 software and complete measurement, by
The diameter of fiber obtained by polymer dope spinning is 100~1000nm；The thickness of the porous nanofiber membrane is 0.05
~5.0mm.In the present invention, if it is all undesirable that fibre diameter and porous nanofiber membrane thickness, which are more than the range,
, because fibre diameter is too small to will affect being uniformly distributed for coloring agent, and diameter can then reduce greatly very much the detection limit of label.Fiber
The thickness of film is too small, is easily damaged；Volatile bio amine cannot permeate rapidly entire film and will appear if tunica fibrosa is too thick
Colo(u)r breakup phenomenon.
Acquired porous nanofiber membrane is by conventional sweep electron microscope analysis, and obtained scanning electron microscope (SEM) photograph is shown in attached drawing 1, by attached
Fig. 1 is it was determined that the diameter of the fiber as obtained by polymer dope spinning is 100~1000nm；The porous nano-fibre
The thickness of film is 0.05~5.0mm.
It is prepared for identifying the visual indicating label of Meat that the invention further relates to the production methods.
The invention further relates to application of the visual indicating label in identification Meat.The visual indicating label with
Meat is stored at 4~30 DEG C of temperature place together, and with the increase of volatile bio amine amount, the color of label is changed by red
Yellowly, fluorescence are changed into hyperfluorescence from unstressed configuration, i.e., according to label color, by red, to yellow, (red-Chinese red-is yellowish
Color-yellow) and fluorescence by by force to the weak freshness that may determine that its meat.Specifically, yellow and fluorescence have shown by force its meat
It is putrid and deteriorated, it is faint yellow with middle intensity fluorescent when, Meat is that three-level is fresh.When Chinese red and micro- fluorescence, meat
Freshness is that second level is fresh.When red and unstressed configuration, the freshness of meat is that level-one is fresh.
Then, which is placed in again on the bottleneck equipped with acid solution, under the action of acid vapors, it
Color original red is transformed by yellow, fluorescence is changed into unstressed configuration from hyperfluorescence, and then it can be reused.It is described
Acid solution be, for example, concentration be 10~50mM hydrochloric acid, sulfuric acid, glacial acetic acid acid solution.
[beneficial effect]
The beneficial effects of the present invention are: porous nanofiber membrane high sensitivity prepared by the present invention, specific recognition is strong, and
And it can reuse.The visual indicating label of the present invention accurately can monitor in real time Meat by naked eyes, be meat
Detection provides a kind of practicable method.
[Detailed description of the invention]
Fig. 1: porous nanofiber membrane scanning electron microscope (SEM) photograph prepared by the present invention.
[specific embodiment]
It will be better understood that the present invention by following embodiments.
Embodiment 1: the visual indicating label production of the present invention
The implementation steps of the embodiment are as follows:
A, spinning solution is prepared
At room temperature, by 0.01 parts by weight by Ai Yijin Biotechnology Co., Ltd with trade name 1,2- dihydro-quinoxaline spreads out
1, the 2- dihydro-quinoxaline derivatives fluorescent indicating dye of biology sale is added in 105 parts by weight Methanol solvents, uses magnetic force
Blender stirs 6h at revolving speed 100rpm, is completely dissolved fluorescent reporter dyes, then adds 30 parts by weight by Shanghai crystalline substance all
The polylactic acid polymer that biological Co., Ltd is sold with trade name polylactic acid, using magnetic stirring apparatus at revolving speed 100rpm
Continue to stir 28h, polymer is completely dissolved, be uniformly mixed, obtains the spinning solution；
B, porous nanofiber membrane is prepared
It works in peace and contentment the electrostatic spinning machine that Developmental Technologies LLC sold with trade name electrostatic spinning machine using by Beijing Yongkang
Under conditions of 40 DEG C of temperature, relative humidity 40%, spinning voltage 10kv, spinning distance 30cm and injection speed 2.0ml/h, allow
The spinning solution that step A is obtained carries out weaving 50min, obtains a kind of porous nanofiber membrane, is then placed in baking oven in temperature 60
Heating removes extra solvent at DEG C, obtains the porous nanofiber membrane.
The porous nanofiber membrane determines that the diameter of fiber is 690nm through scanning electron microscope analysis；The porous nano is fine
The thickness for tieing up film is 4.2mm；
The label that the present invention uses is that tunica fibrosa is cut into the circular film that diameter is 1cm, obtains the visual instruction
Label.
Embodiment 2: the visual indicating label production of the present invention
The implementation steps of the embodiment are as follows:
A, spinning solution is prepared
At room temperature, 0.1 parts by weight are sold by Guangzhou Bo Bai trade Co., Ltd with trade name Rhodamine Derivatives
Rhodamine Derivatives fluorescent reporter dyes are added in 90 parts by weight chloroform solvents, using magnetic stirring apparatus in revolving speed
5h is stirred under 300rpm, is completely dissolved fluorescent reporter dyes, then adds 12 parts by weight by Shanghai Aladdin biochemical technology stock
The poly styrene polymer that part Co., Ltd is sold with trade name polystyrene, using magnetic stirring apparatus at revolving speed 10rpm after
Continuous stirring 30h, polymer is completely dissolved, is uniformly mixed, the spinning solution is obtained；
B, porous nanofiber membrane is prepared
It works in peace and contentment the electrostatic spinning machine that Developmental Technologies LLC sold with trade name electrostatic spinning machine using by Beijing Yongkang
Under conditions of temperature 50 C, relative humidity 60%, spinning voltage 20kv, spinning distance 8cm and injection speed 6.0ml/h, allow
The spinning solution that step A is obtained carries out weaving 30min, obtains a kind of porous nanofiber membrane, is then placed in baking oven in temperature 70
Heating removes extra solvent at DEG C, obtains the porous nanofiber membrane.
The porous nanofiber membrane determines that the diameter of fiber is 100nm through scanning electron microscope analysis；The porous nano is fine
The thickness for tieing up film is 0.05mm；
The label that the present invention uses is that tunica fibrosa is cut into the circular film that diameter is 1cm, obtains the visual instruction
Label.
Embodiment 3: the visual indicating label production of the present invention
The implementation steps of the embodiment are as follows:
A, spinning solution is prepared
At room temperature, two acyls 10 parts by weight sold by BOC Sciences company with trade name imidodicarbonic diamide are sub-
Amine fluorescent reporter dyes are added in 110 parts by weight N, N- dimethylformamide solvents, using magnetic stirring apparatus in revolving speed 600rpm
Lower stirring 4h, is completely dissolved fluorescent reporter dyes, then add 5 parts by weight by Shanghai Mike woods biochemical technology Co., Ltd with
The polyethylene oxide polymer of trade name polyethylene oxide sale, continues to stir using magnetic stirring apparatus at revolving speed 1000rpm
Polymer is completely dissolved by 20h, is uniformly mixed, and the spinning solution is obtained；
B, porous nanofiber membrane is prepared
It works in peace and contentment the electrostatic spinning machine that Developmental Technologies LLC sold with trade name electrostatic spinning machine using by Beijing Yongkang
Under conditions of 20 DEG C of temperature, relative humidity 20%, spinning voltage 2kv, spinning distance 16cm and injection speed 0.2ml/h, allow
The spinning solution that step A is obtained carries out weaving 60min, obtains a kind of porous nanofiber membrane, is then placed in baking oven in temperature 100
Heating removes extra solvent at DEG C, obtains the porous nanofiber membrane.
The porous nanofiber membrane determines that the diameter of fiber is 380nm through scanning electron microscope analysis；The porous nano is fine
The thickness for tieing up film is 1.2mm；
The label that the present invention uses is that tunica fibrosa is cut into the circular film that diameter is 1cm, obtains the visual instruction
Label.
Embodiment 4: the visual indicating label production of the present invention
The implementation steps of the embodiment are as follows:
A, spinning solution is prepared
At room temperature, 5 parts by weight are sold by Suzhou Xing Shuo nanosecond science and technology Co., Ltd with trade name CdSe/ZnSQDs
Quantum dot fluorescence indicating dye is added in 95 parts by weight dimethylsulfoxide solvents, using magnetic stirring apparatus at revolving speed 1000rpm
2h is stirred, fluorescent reporter dyes are completely dissolved, then adds 22 parts by weight by all biological Co., Ltd of Shanghai crystalline substance with quotient
The polyvinyl pyrrolidone polymers of name of an article polyvinylpyrrolidone sale, are continued at revolving speed 500rpm using magnetic stirring apparatus
For 24 hours, polymer is completely dissolved for stirring, is uniformly mixed, and the spinning solution is obtained；
B, porous nanofiber membrane is prepared
It works in peace and contentment the electrostatic spinning machine that Developmental Technologies LLC sold with trade name electrostatic spinning machine using by Beijing Yongkang
Under conditions of 30 DEG C of temperature, relative humidity 90%, spinning voltage 40kv, spinning distance 24cm and injection speed 10.0ml/h,
The spinning solution for allowing step A to obtain carries out weaving 40min, obtains a kind of porous nanofiber membrane, is then placed in baking oven in temperature
Heating removes extra solvent at 86 DEG C, obtains the porous nanofiber membrane.
The porous nanofiber membrane determines that the diameter of fiber is 1000nm through scanning electron microscope analysis；The porous nano
The thickness of tunica fibrosa is 5.0mm；
The label that the present invention uses is that tunica fibrosa is cut into the circular film that diameter is 1cm, obtains the visual instruction
Label.
Test example 1: detection of the visual indicating label of the present invention to mutton freshness
The embodiment of the test example is as follows:
Test specimen: porous nano fluorescent fiber film prepared by embodiment 1
New fresh mutton pretreatment: new fresh mutton is removed into epidermis, tendon, fat etc., obtains quality 10g mutton chop sample.It will
Meat piece is put into culture dish.
Fresh meat samples packing processes:
Porous nano fluorescent fiber film prepared by embodiment 1 is cut into round of diameter 2cm.Will pretreatment mutton chop with
Porous nano fluorescent fiber film is put into culture dish simultaneously, and culture dish preservative film environmental sealing covers culture ware lid, then be placed in
It is stored at 25 DEG C of temperature in constant incubator.
As quality of mutton changes, mutton generates a large amount of volatile bio amine under microbial action, causes porous receive
Rice fluorescent fiber film color becomes yellow from red, and fluorescence is from nothing to hyperfluorescence, it was demonstrated that porous nano fluorescent fiber film of the present invention
Ability with real-time visual detection Meat.
The taking-up of used porous nano fluorescent fiber film the hydrochloric acid solution bottleneck that concentration is 30mM is put in again to fumigate,
Label color becomes red from yellow again, it was demonstrated that porous nano fluorescent fiber film of the present invention is reusable.
Test example 2: detection of the visual indicating label of the present invention to mutton freshness
The embodiment of the test example is identical as the embodiment of test example 1, is only prepared using embodiment 3
Porous nano fluorescent fiber film and fresh beef.The observed result of the test example is identical as test example 1.
Claims (10)
1. a kind of for identifying the visual indicating label production method of Meat, it is characterised in that the step of the production method
It is as follows:
A, spinning solution is prepared
At room temperature, 0.01~10 parts by weight fluorescent reporter dyes are added in 90~110 parts by weight solvent, are stirred using magnetic force
Device stirring is mixed, fluorescent reporter dyes are completely dissolved, then adds 5~30 parts by weight polymer, using magnetic stirrer,
Polymer is completely dissolved, is uniformly mixed, the spinning solution is obtained；
B, porous nanofiber membrane is prepared
Using electrostatic spinning machine 20~50 DEG C of temperature, relative humidity 20~90%, 2~40kv of spinning voltage, spinning distance 8~
Under conditions of 30cm and 0.2~10.0ml/h of injection speed, the spinning solution for allowing step A to obtain carries out weaving 30-60min, obtains
A kind of porous nanofiber membrane is then placed in baking oven the heating at temperature 60 C~100 DEG C and removes extra solvent, obtains institute
The porous nanofiber membrane stated；The porous nanofiber membrane is cut into the circular film of diameter 1cm, obtains the visual instruction
Label.
2. manufacturing method according to claim 1, it is characterised in that the weight ratio of fluorescent reporter dyes, polymer and solvent
It is 0.1~8.0:10~25:95~105.
3. production method according to claim 1 or 2, it is characterised in that the fluorescent reporter dyes are one or more
Fluorescent reporter dyes selected from 1,2- dihydro-quinoxaline derivative, Rhodamine Derivatives, imidodicarbonic diamide or quantum dot.
4. production method according to claim 1 or 2, it is characterised in that the polymer is one or more selected from poly-
Lactic acid, polystyrene, acetate fiber, polyethylene oxide, polystyrene, polycaprolactone or polyvinylpyrrolidone polymer.
5. production method according to claim 1 or 2, it is characterised in that the solvent is one or more selected from first
Alcohol, ethyl alcohol, trifluoroacetic acid, acetone, chloroform, ethyl alcohol, N, the solvent of N- dimethylformamide or dimethyl sulfoxide.
6. manufacturing method according to claim 1, it is characterised in that the fluorescent reporter dyes are in the solvent
2~6h is stirred at 100~1000rpm of revolving speed.
7. manufacturing method according to claim 1, it is characterised in that after the addition polymer revolving speed 10~
Continue 20~30h of stirring under 1000rpm.
8. manufacturing method according to claim 1, it is characterised in that the fiber as obtained by polymer dope spinning it is straight
Diameter is 100~1000nm；The thickness of the porous nanofiber membrane is 0.05~5.0mm.
9. production method described in any one of -8 claims is prepared for identifying Meat according to claim 1
Visual indicating label.
10. application of the visual indicating label in identification Meat according to claim 9, it is characterised in that can by this
Store the freshness of real-time monitoring meat at 4~30 DEG C of temperature together with meat depending on indicating label；With volatility in packing
The increase of biogenic amine amount, its color change yellowly by red, and fluorescence is changed into hyperfluorescence from unstressed configuration, according to color with
Fluorescence intensity judges its Meat；Then, which is placed in again on the bottleneck equipped with acid solution, in acidity
Under the action of steam, its color is transformed into original red by yellow, and fluorescence is changed into unstressed configuration from hyperfluorescence, then it
It can reuse.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110618116A (en) * | 2019-08-28 | 2019-12-27 | 江苏大学 | Preparation method and application of intelligent indication label for visually detecting freshness of meat |
| CN111472092A (en) * | 2020-04-22 | 2020-07-31 | 内蒙古农业大学 | Double-indication intelligent label for identifying meat quality and manufacturing method and application thereof |
| CN111472102A (en) * | 2020-04-13 | 2020-07-31 | 内蒙古农业大学 | Intelligent label capable of identifying and prolonging meat quality and manufacturing method and application thereof |
| CN111825988A (en) * | 2020-06-19 | 2020-10-27 | 大连工业大学 | Collagen fiber-based membrane material for rapidly detecting biogenic amine and preparation method thereof |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19708492A1 (en) * | 1997-03-03 | 1998-09-10 | Technologisches Beratungs Und | Visual indicator |
| CN105324667A (en) * | 2013-06-19 | 2016-02-10 | 柯尼卡美能达株式会社 | Fluorescent nanoparticles for biomolecular staining and manufacturing method for same |
| CN106433618A (en) * | 2016-09-21 | 2017-02-22 | 天津工业大学 | Preparation method for fluorescent colorimetric chemical sensor and application of fluorescent colorimetric chemical sensor in gas detection |
| CN106442497A (en) * | 2016-09-21 | 2017-02-22 | 天津工业大学 | Preparation method of porphyrin/nano porous fiber membrane hydrogen chloride gas sensor |
| CN109111913A (en) * | 2017-06-23 | 2019-01-01 | 中国科学院化学研究所 | A kind of pair of transmitting cellulose base fluorescent material and its preparation method and application |
| CN109252293A (en) * | 2018-09-03 | 2019-01-22 | 内蒙古农业大学 | A kind of nano polymer fibers film and preparation method thereof and purposes |
-
2019
- 2019-04-10 CN CN201910283409.1A patent/CN109975283A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19708492A1 (en) * | 1997-03-03 | 1998-09-10 | Technologisches Beratungs Und | Visual indicator |
| CN105324667A (en) * | 2013-06-19 | 2016-02-10 | 柯尼卡美能达株式会社 | Fluorescent nanoparticles for biomolecular staining and manufacturing method for same |
| CN106433618A (en) * | 2016-09-21 | 2017-02-22 | 天津工业大学 | Preparation method for fluorescent colorimetric chemical sensor and application of fluorescent colorimetric chemical sensor in gas detection |
| CN106442497A (en) * | 2016-09-21 | 2017-02-22 | 天津工业大学 | Preparation method of porphyrin/nano porous fiber membrane hydrogen chloride gas sensor |
| CN109111913A (en) * | 2017-06-23 | 2019-01-01 | 中国科学院化学研究所 | A kind of pair of transmitting cellulose base fluorescent material and its preparation method and application |
| CN109252293A (en) * | 2018-09-03 | 2019-01-22 | 内蒙古农业大学 | A kind of nano polymer fibers film and preparation method thereof and purposes |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110618116A (en) * | 2019-08-28 | 2019-12-27 | 江苏大学 | Preparation method and application of intelligent indication label for visually detecting freshness of meat |
| CN111472102A (en) * | 2020-04-13 | 2020-07-31 | 内蒙古农业大学 | Intelligent label capable of identifying and prolonging meat quality and manufacturing method and application thereof |
| CN111472092A (en) * | 2020-04-22 | 2020-07-31 | 内蒙古农业大学 | Double-indication intelligent label for identifying meat quality and manufacturing method and application thereof |
| CN111472092B (en) * | 2020-04-22 | 2022-04-26 | 内蒙古农业大学 | Double-indication intelligent label for identifying meat quality and manufacturing method and application thereof |
| CN111825988A (en) * | 2020-06-19 | 2020-10-27 | 大连工业大学 | Collagen fiber-based membrane material for rapidly detecting biogenic amine and preparation method thereof |
| CN112662331A (en) * | 2020-12-18 | 2021-04-16 | 滕德辉 | Cold chain transportation safety label sticker and production process thereof |
| CN113281312A (en) * | 2021-03-31 | 2021-08-20 | 渤海大学 | Preparation method and application of ratio-type fluorescent response sensing tag for freshness of salmon |
| CN113281312B (en) * | 2021-03-31 | 2024-02-02 | 渤海大学 | Preparation method and application of ratio type fluorescence response sensing tag for salmon freshness |
| CN115171515A (en) * | 2022-07-08 | 2022-10-11 | 华南理工大学 | Label reflecting food freshness and method for detecting food freshness |
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