CN109966490A - A kind of degradable antimony nanostructure, preparation method and application - Google Patents
A kind of degradable antimony nanostructure, preparation method and application Download PDFInfo
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Abstract
It is to load anticancer drug doxorubicin hydrochloride in degradable antimony nanoparticle surface the invention discloses a kind of degradable antimony nanostructure；Its antimony nanoparticle has electronegativity, electrostatic interaction is carried out with positively charged doxorubicin hydrochloride, and on the surface of the nanostructure of formation through the further modified of organic ligand, with good water solubility；And the nanostructure is whole degradable under the laser emission of setting.The invention also discloses the preparation methods of the structure, use direct-reduction process, make nanometer particle load doxorubicin hydrochloride anticancer drug.It can be applied to chemotherapy and photo-thermal physical therapy.Nanostructured morphologies provided by the invention are uniform, size uniformity, good biocompatibility, have high drug loading rate and photothermal conversion efficiency, and have degradable characteristic.The nanostructure can be used as preparation, be widely used in chemotherapy and photo-thermal physical therapy, and synergistic effect can greatly improve therapeutic efficiency.
Description
Technical field
The present invention relates to Bio-Nano-Materials technical fields, and in particular to it is a kind of can for cooperate with chemotherapy and photo-thermal physical therapy
Degradation antimony nanostructure, preparation method and application.
Background technique
Nowadays, health has been to be concerned by more and more people, however all the time, cancer is still to lead to the mankind
Dead one of the major reasons.Currently, chemotherapy and radioactivity radiotherapy are the most important treatment methods for the treatment of cancer, it is preceding
Person, long-term treatment can generate drug resistance, and the latter's radioactive radiation is very big to body side effect, this all strongly limits controlling for they
Therapeutic effect.And photo-thermal therapy method is the material that will have high light thermal conversion efficiency, passes through the side of active targeting or passive target
Formula is introduced into tumor tissues, then in the case where the irradiation of external light source is penetrated, convert light into as heat, increases to kill by temperature
A kind of cancer treatment method of cancer cell.Currently, various types of photothermal conversion Bio-Nano-Materials have been widely used in
Photo-thermal physical therapy.
However, single photo-thermal physical therapy mode, therapeutic effect still complies with one's wishes not to the utmost, and therefore, researchers attempt photo-thermal
Physical therapy is combined from different treatment methods (such as chemotherapy, photodynamic therapy and radiotherapy etc.), to improve treatment effect
Fruit.Wherein, chemotherapy is more paid close attention to as presently the most effective and extensive Cancer Treatment Regimens by people, studies have shown that
It, can be effectively for cooperateing with chemotherapy and photo-thermal physical therapy using single nanostructure compared with individual photo-thermal physical therapy or amic therapy method
Enhance therapeutic efficiency.In addition, the bio-toxicity of nano material and the emphasis of scientists study.Nano material is in blood
Residence time is longer, bigger to the toxicity of biological tissue, but needs it to keep sufficiently long blood circulation time simultaneously again,
To ensure therapeutic effect, therefore develop the attention that biodegradable efficient nano material also greatly causes people.
It, not only can be with currently, the degradable nano material for synergistic treatment of exploitation, has a good application prospect
The effect for the treatment of is effectively improved, and biomaterial bring toxicity can be substantially reduced, is become the focus of people's research and emphasis.
But the collaboration chemistry and photo-thermal physical therapy nanostructure of prior art method preparation, with structure is complicated, unstable
It is fixed, and water-soluble and poor biocompatibility and the disadvantages of can not degrading, keep its application limited；Also, conventional preparation method work
Sequence complexity, raw material and equipment price are high, are not easy to realization and stably produce.
Summary of the invention
The present invention be directed to the deficiencies in the prior art, and provide a kind of stable structure, and water-soluble and good biocompatibility,
Degradable antimony nanostructure with high photothermal conversion efficiency and drug loading rate can to overcome existing product disadvantage
Meet the demand of the clinical synergistic treatment for diseases such as cancers, improves therapeutic effect, expanded application field.
Present invention simultaneously provides a kind of methods for preparing the degradable antimony nanostructure, using direct-reduction process, to overcome
Conventional preparation method complex procedures, raw material and equipment price are high, are not easy to realize the problem of stably producing.
The present invention also provides the application of the degradable antimony nanostructure as diagnosis and treatment preparation in biologic medical field.
To achieve the above object, the technical solution adopted by the present invention is that:
A kind of degradable antimony nanostructure, which is characterized in that the nanostructure is in degradable antimony nanoparticle surface
Load anticancer drug doxorubicin hydrochloride；Its antimony nanoparticle has electronegativity, carries out electrostatic with positively charged doxorubicin hydrochloride
Effect, and on the surface of the nanostructure of formation through the further modified of organic ligand, with good water solubility；And
The nanostructure is whole degradable under the laser emission of setting.
A kind of preparation method of aforementioned degradable antimony nanostructure, which comprises the following steps:
1) it using sodium borohydride as reducing agent, is dissolved in n-methyl-2-pyrrolidone (NMP), after ultrasonic disperse, is placed in oil
It in bath, is dispersed with stirring, obtains the first dispersion liquid；
2) antimony chloride is dissolved in n-methyl-2-pyrrolidone, is added in the first dispersion liquid, it is lasting to stir, to its crystalline substance
Body grows to obtain antimony nanoparticle, obtains the second dispersion liquid；
3) resulting second dispersion liquid is mixed with doxorubicin hydrochloride (DOX) anticancer drug, it is lasting to stir, obtain hydrochloric acid Ah mould
The antimony nanostructure of element load, obtains third dispersion liquid；
4) water-soluble surface organic ligands and third dispersion liquid are thoroughly mixed to get to being used for collaborative
The degradable antimony nanostructure with photo-thermal physical therapy is treated, is to load doxorubicin hydrochloride anticancer drug institute shape in antimony nanoparticle surface
At degradable nano structure.
First dispersion liquid specific steps described in step 1) are as follows: 280-300mg sodium borohydride is dissolved in 25mL N- methyl-
In 2-Pyrrolidone, ultrasonic disperse 30min is placed it in oil bath pan, is stirred continuously until that temperature rises to 60 DEG C, is obtained first
Dispersion liquid.
The second dispersion liquid of step 2) specific steps are as follows: 3.5-4.0g antimony chloride ultrasound is first dissolved in 5mL N- methyl-
It in 2-Pyrrolidone, is then quickly adding into the first dispersion liquid, is uniformly mixed, persistently stirs 2h, centrifuge washing disperses again
In 10mL deionized water, the second dispersion liquid is obtained.
The specific steps of the step 3) are as follows: 8-8.1mg DOX is dissolved in 4mL deionized water, it is mixed with the second dispersion liquid
It closes uniformly, after being protected from light and being stirred overnight, centrifuge washing, then be scattered in ultrapure water again, obtain third dispersion liquid.
The specific steps of the step 4) are as follows: the surface organic ligands polyacrylic acid (PAA) of 50-60mg is dissolved in 10mL
In ionized water, after ultrasonic disperse, it is thoroughly mixed with third dispersion liquid, centrifuge washing is to get to for cooperateing with chemotherapy and light
The degradable antimony nanostructure of hot physical therapy.
Water-soluble surface organic ligands include: polyvinylpyrrolidone (PVP), polyethylene glycol in the step 4)
(PEG), polyacrylic acid (PAA), polyethyleneimine (PEI).
The degradable antimony nanostructure, which is characterized in that as diagnosis and treatment preparation, be applied to collaboration chemotherapy and photo-thermal
Physical therapy.
The beneficial effects of the present invention are:
(1) provided by the present invention for the preparation method of collaboration chemotherapy and the degradable antimony nanostructure of photo-thermal physical therapy, lead to
The control to crucial component, proportion is crossed, keeps nanostructured morphologies provided by the invention uniform, size uniformity, biocompatibility
It is good, there is high drug loading rate and photothermal conversion efficiency, and there is whole degradable characteristic.Using the nanostructure as system
Agent greatly improves therapeutic efficiency for the synergistic effect of chemotherapy and photo-thermal physical therapy.
(2) preparation method of degradable antimony nanostructure provided by the invention utilizes sodium borohydride using direct-reduction process
Reproducibility, reduction obtains antimony nanoparticle, after loading doxorubicin hydrochloride drug, obtains for cooperateing with chemotherapy and photo-thermal physical therapy
Degradable antimony nanostructure.The preparation method effectively overcomes the deficiency of existing method, preparation flow is simple, concise in technology,
Reaction condition is mild, process is easily controllable, reproducible, and equipment is simple, at low cost, resulting nano junction nano junction configuration
Looks are uniform, size uniformity, good biocompatibility；And there is high drug loading rate and photothermal conversion efficiency, yield and quality are more
Stablize, is easy to industrialization.
(3) degradable antimony nanostructure provided by the invention and preparation method thereof, it is preferred that emphasis is utilize the strong of sodium borohydride
Reproducibility is reduced directly antimony chloride, obtains antimony nanoparticle, with good laser emission degradability, loads hydrochloric acid Ah
After mycin, there is the nanostructure of chemistry and photo-thermal physiotherapy function simultaneously, effectively increase therapeutic effect, extend this and receive
The application range of rice structure.
(4) degradable antimony nanostructure provided by the invention, as diagnosis and treatment preparation for cooperateing with chemotherapy and photo-thermal physical therapy
When, photothermal conversion efficiency is up to 40-50%, and drug loading rate is up to 26.5%；Making full use of the high photothermal conversion of nanostructure
While efficiency and drug loading rate, so that it is had the characteristic degradable in the case where setting laser emission, make the nanostructure
With whole degradable characteristic, bio-toxicity is reduced, has widened it in the application prospect of field of biomedicine.
With reference to the accompanying drawing with specific embodiment, the present invention is described in more detail.
Detailed description of the invention
Fig. 1 is the TEM of the degradable antimony nanostructure obtained for cooperateing with chemotherapy and photo-thermal physical therapy of the embodiment of the present invention 1
Photo；
Fig. 2 is the heat of the degradable antimony nanostructure obtained for cooperateing with chemotherapy and photo-thermal physical therapy of the embodiment of the present invention 1
Images；
Fig. 3 is the medicine of the degradable antimony nanostructure obtained for cooperateing with chemotherapy and photo-thermal physical therapy of the embodiment of the present invention 1
Object release profiles；
Fig. 4 is the thin of the degradable antimony nanostructure obtained for cooperateing with chemotherapy and photo-thermal physical therapy of the embodiment of the present invention 1
Born of the same parents' survival rate.
Fig. 5 is the purple of the degradable antimony nanostructure obtained for cooperateing with chemotherapy and photo-thermal physical therapy of the embodiment of the present invention 1
Outside-visible absorption spectra (degradation rate).
Specific embodiment
Embodiment 1
Referring to attached drawing 1~5, degradable antimony nanostructure provided in this embodiment, which is in degradable antimony
Nanoparticle surface loads anticancer drug doxorubicin hydrochloride；Its antimony nanoparticle has electronegativity, with positively charged hydrochloric acid Ah
Mycin carries out electrostatic interaction, and on the surface of the nanostructure of formation through the further modified of organic ligand, with good
Good water solubility；And the nanostructure is whole degradable under the laser emission of setting.
The preparation method of aforementioned degradable antimony nanostructure, core be using direct-reduction process synthesize antimony nanoparticle, then
Doxorubicin hydrochloride anticancer drug is loaded, the method system for cooperateing with the degradable antimony nanostructure of chemotherapy and photo-thermal physical therapy is obtained
It is standby, comprising the following steps:
1) it using sodium borohydride as reducing agent, is dissolved in n-methyl-2-pyrrolidone (NMP), after ultrasonic disperse, is placed in oil
It in bath, is dispersed with stirring, obtains the first dispersion liquid；
Specifically: sodium borohydride being dissolved in n-methyl-2-pyrrolidone, ultrasonic disperse 30min places it in oil bath pan
In, it is stirred continuously until that temperature rises to 60 DEG C, obtains the first dispersion liquid.
2) antimony chloride is dissolved in n-methyl-2-pyrrolidone, is added in the first dispersion liquid, it is lasting to stir, to its crystalline substance
Body grows to obtain antimony nanoparticle, obtains the second dispersion liquid；
Specifically: first antimony chloride ultrasound being dissolved in n-methyl-2-pyrrolidone, is then quickly adding into the first dispersion liquid
In, it is uniformly mixed, persistently stirs 2h, centrifuge washing is scattered in deionized water again, obtains the second dispersion liquid.
3) resulting second dispersion liquid is mixed with doxorubicin hydrochloride (DOX) anticancer drug, it is lasting to stir, obtain hydrochloric acid Ah mould
The antimony nanostructure of element load, obtains third dispersion liquid；
Specifically: DOX is dissolved in deionized water, is uniformly mixed with the second dispersion liquid, after being protected from light and being stirred overnight, centrifugation
Washing, then be scattered in ultrapure water again, obtain third dispersion liquid.
4) water-soluble surface organic ligands and third dispersion liquid are thoroughly mixed to get to being used for collaborative
The degradable antimony nanostructure with photo-thermal physical therapy is treated, is to load doxorubicin hydrochloride anticancer drug institute shape in antimony nanoparticle surface
At degradable nano structure.
Specifically: surface organic ligands polyacrylic acid (PAA) being dissolved in deionized water, after ultrasonic disperse, with third point
Dispersion liquid is thoroughly mixed, and centrifuge washing is to get to for cooperateing with the degradable antimony nanostructure of chemotherapy and photo-thermal physical therapy.
Water-soluble surface organic ligands include: polyvinylpyrrolidone (PVP), polyethylene glycol in the step 4)
(PEG), polyacrylic acid (PAA), polyethyleneimine (PEI).
More specifically:
(1) it by 300mg sodium borohydride, is dissolved in 25mL n-methyl-2-pyrrolidone, ultrasonic disperse 30min is set
It in oil bath pan, is stirred continuously until that temperature rises to 60 DEG C, after maintaining 30min at such a temperature, obtains the first dispersion liquid.
(2) 3.5g antimony chloride is dissolved in 5mL n-methyl-2-pyrrolidone, is then quickly adding into the first dispersion liquid
In, it is uniformly mixed, 2h is persistently stirred at 60 DEG C, centrifuge washing is scattered in again in 10mL ionized water, obtains the second dispersion
Liquid.
(3) 8mgDOX being dissolved in 4mL deionized water, is uniformly mixed with the second dispersion liquid, the pH for adjusting solution is 7.5,
After being protected from light and being stirred overnight, centrifuge washing, then be scattered in ultrapure water again, obtain third dispersion liquid.
(4) the surface organic ligands polyacrylic acid (PAA) of 50mg is dissolved in 10mL deionized water, after ultrasonic disperse, with
Third dispersion liquid is thoroughly mixed, and centrifuge washing is to get to for cooperateing with the degradable antimony nano junction of chemotherapy and photo-thermal physical therapy
Structure.
Degradable antimony nanostructure manufactured in the present embodiment, as diagnosis and treatment preparation for cooperateing with chemotherapy and photo-thermal physical therapy
When, through experimental test, photothermal conversion efficiency is up to 40-50%, and drug loading rate is up to 26.5%；Making full use of nano junction
While the high photothermal conversion efficiency of structure and drug loading rate, also have it whole degradable under the laser emission of setting
Characteristic reduces bio-toxicity, has widened it in the application prospect of field of biomedicine.
It is a kind of for cooperateing with the degradable antimony nanometer of chemotherapy and photo-thermal physical therapy made from present invention implementation 1 referring to Fig. 1
The TEM photo of structure, as can be seen from the figure nanostructure good dispersion, pattern is uniform, illustrates that this method can be effectively controlled and receives
The monodispersity and pattern of rice structure, the nanostructure average grain diameter after Organic ligand modification are~40nm, are hydrated grain through being about
110nm, because the nano particle of small size is easier to be conducive to recycle in vivo, be controlled for biology by cell endocytic
It treats significant.
Fig. 2 is in collaboration chemotherapy and the degradable antimony nanostructure of photo-thermal physical therapy obtained by the embodiment of the present invention 1 for light
The photo of thermal imaging.After 808nm laser emission, as sample concentration increases, the temperature of sample is gradually risen, and maximum sample is dense
Raising temperature (△ T) under the conditions of degree can reach 31.2 DEG C or so, by can be calculated photothermal conversion efficiency up to 40-50%,
Illustrate that the nanometer system has good photo-thermal effect.
Fig. 3 is obtained by the embodiment of the present invention 1 for cooperateing with the drug of the degradable antimony nanostructure of chemotherapy and photo-thermal physical therapy
Release profiles.It can be seen from the figure that prepared nanostructure has the drug release characteristics of pH response, in acid condition
(pH 5.0), doxorubicin hydrochloride discharge more；And laser irradiation can be further improved release amount of medicine.This is because swashing
Light irradiation can cause antimony nanostructure that must degrade, to further increase drug release rate.
Fig. 4 be obtained by the embodiment of the present invention 1 for cooperate with chemotherapy and the degradable antimony nanostructure of photo-thermal physical therapy from it is different
Cell survival rate after second, third dispersion liquid culture of concentration.From figure, by control group, it will be seen that working as
When HeLa cell and the second dispersion liquid and third dispersion liquid are hatched, independent photo-thermal physical therapy discharges bring chemotherapy than drug alone
The effect of effect will be got well, and cell survival rate is lower；It will be appreciated that the cell survival rate of the third dispersion liquid under illumination
It is minimum, illustrate that the nanometer system shows the therapeutic effect of good collaboration chemotherapy and photo-thermal physical therapy.
Fig. 5 is that the embodiment of the present invention 1 is resulting for cooperateing with the degradable antimony nanostructure of chemotherapy and photo-thermal physical therapy not having
Useful laser and with the uv-visible absorption spectra after laser emission.It can be seen from the figure that with 808nm laser emission 5-
After 10min, absorbance of the nanostructure at 500-800nm is decreased obviously, and illustrates nanostructure after illumination, structure has occurred
Degradation, so that absorbance declines.
Embodiment 2
Degradable antimony nanostructure provided in this embodiment, preparation method and applications, it is substantially the same manner as Example 1,
The difference is that comprising following steps:
(1) it by 280mg sodium borohydride, is dissolved in 25mL n-methyl-2-pyrrolidone, ultrasonic disperse 30min is set
It in oil bath pan, is stirred continuously until that temperature rises to 60 DEG C, after maintaining 30min at such a temperature, obtains the first dispersion liquid.
(2) 3.8g antimony chloride is dissolved in 5mL n-methyl-2-pyrrolidone, is then quickly adding into the first dispersion liquid
In, it is uniformly mixed, 2h is persistently stirred at 60 DEG C, centrifuge washing is scattered in again in 10mL deionized water, obtains the second dispersion
Liquid.
(3) 8.1mg DOX is dissolved in 4mL deionized water, is uniformly mixed with the second dispersion liquid, the pH for adjusting solution is
7.5, after being protected from light and being stirred overnight, centrifuge washing, then be scattered in ultrapure water again, obtain third dispersion liquid.
(4) the surface organic ligands polyvinylpyrrolidone (PVP) of 55mg is dissolved in 10mL deionized water, ultrasonic disperse
Afterwards, it is thoroughly mixed with third dispersion liquid, centrifuge washing is to get to for cooperateing with the degradable antimony of chemotherapy and photo-thermal physical therapy to receive
Rice structure.
Embodiment 3
Degradable antimony nanostructure provided in this embodiment, preparation method and applications, with embodiment 1,2 basic phases
Together, the difference is that comprising following steps:
(1) it by 290mg sodium borohydride, is dissolved in 25mL n-methyl-2-pyrrolidone, ultrasonic disperse 30min is set
It in oil bath pan, is stirred continuously until that temperature rises to 60 DEG C, after maintaining 30min at such a temperature, obtains the first dispersion liquid.
(2) 3.5g antimony chloride is dissolved in 5mL n-methyl-2-pyrrolidone, is then quickly adding into the first dispersion liquid
In, it is uniformly mixed, 2h is persistently stirred at 60 DEG C, centrifuge washing is scattered in again in 10mL deionized water, obtains the second dispersion
Liquid.
(3) 8mg DOX being dissolved in 4mL deionized water, is uniformly mixed with the second dispersion liquid, the pH for adjusting solution is 7.5,
After being protected from light and being stirred overnight, centrifuge washing, then be scattered in ultrapure water again, obtain third dispersion liquid.
(4) the surface organic ligands polyethylene glycol (PEG) of 50mg is dissolved in 10mL deionized water, after ultrasonic disperse, with
Third dispersion liquid is thoroughly mixed, and centrifuge washing is to get to for cooperateing with the degradable antimony nano junction of chemotherapy and photo-thermal physical therapy
Structure.
Embodiment 4
Degradable antimony nanostructure provided in this embodiment, preparation method and applications, it is basic with Examples 1 to 3
It is identical, the difference is that comprising following steps:
1) it by 280mg sodium borohydride, is dissolved in 25mL n-methyl-2-pyrrolidone, ultrasonic disperse 30min is placed it in
It in oil bath pan, is stirred continuously until that temperature rises to 60 DEG C, after maintaining 30min at such a temperature, obtains the first dispersion liquid.
(2) 3.9g antimony chloride is dissolved in 5mL n-methyl-2-pyrrolidone, is then quickly adding into the first dispersion liquid
In, it is uniformly mixed, 2h is persistently stirred at 60 DEG C, centrifuge washing is scattered in again in 10mL deionized water, obtains the second dispersion
Liquid.
(3) 8.1mg DOX is dissolved in 4mL deionized water, is uniformly mixed with the second dispersion liquid, the pH for adjusting solution is
7.5, after being protected from light and being stirred overnight, centrifuge washing, then be scattered in ultrapure water again, obtain third dispersion liquid.
(4) the surface organic ligands polyethyleneimine (PEI) of 60mg is dissolved in 10mL deionized water, after ultrasonic disperse,
It is thoroughly mixed with third dispersion liquid, centrifuge washing is to get to for cooperateing with the degradable antimony nanometer of chemotherapy and photo-thermal physical therapy
Structure.
Of the invention focuses on, provided by the present invention for cooperateing with the degradable antimony nanostructure of chemotherapy and photo-thermal physical therapy
And preparation method thereof, by the control to crucial component, proportion, using direct-reduction process, using the reproducibility of sodium borohydride,
Reduction obtains antimony nanoparticle, after loading doxorubicin hydrochloride drug, obtains the degradable antimony for cooperateing with chemotherapy and photo-thermal physical therapy
Nanostructure.The preparation method effectively overcomes the deficiency of existing method, preparation process flow is succinct, reaction condition is mild,
Process is easily controllable, reproducible, and equipment investment is few, at low cost, and resulting nano junction nanostructured morphologies are uniform, size
It is uniform, good biocompatibility, and there is high drug loading rate and photothermal conversion efficiency, yield and quality are relatively stable, are easy to produce
Industry.
Present invention is not limited to the embodiments described above, obtained other for cooperateing with using same or similar method
Chemotherapy and the degradable antimony nanostructure of photo-thermal physical therapy and preparation method thereof such as use different organic ligand (polyacrylic acid
(PAA), polyvinylpyrrolidone (PVP), polyethylene glycol (PEG), polyethyleneimine (PEI)) etc., in the scope of the present invention
It is interior.
Claims (8)
1. a kind of degradable antimony nanostructure, which is characterized in that the nanostructure is negative in degradable antimony nanoparticle surface
Carried anticancer medicine object doxorubicin hydrochloride；Its antimony nanoparticle has electronegativity, carries out electrostatic work with positively charged doxorubicin hydrochloride
With, and on the surface of the nanostructure of formation through the further modified of organic ligand, with good water solubility；And it should
Nanostructure is whole degradable under the laser emission of setting.
2. the preparation method of degradable antimony nanostructure according to claim 1, which comprises the following steps:
1) it using sodium borohydride as reducing agent, is dissolved in n-methyl-2-pyrrolidone, after ultrasonic disperse, is placed in oil bath pan, stirs
It mixes uniformly, obtains the first dispersion liquid；
2) antimony chloride is dissolved in n-methyl-2-pyrrolidone, is added in the first dispersion liquid, it is lasting to stir, it is raw to its crystal
Length obtains antimony nanoparticle, obtains the second dispersion liquid；
3) resulting second dispersion liquid is mixed with doxorubicin hydrochloride anticancer drug, it is lasting to stir, obtain doxorubicin hydrochloride load
Antimony nanostructure obtains third dispersion liquid；
4) by water-soluble surface organic ligands and third dispersion liquid be thoroughly mixed to get to be used to cooperateing with chemotherapy and
The degradable antimony nanostructure of photo-thermal physical therapy is formed by antimony nanoparticle surface load doxorubicin hydrochloride anticancer drug
Degradable nano structure.
3. the preparation method of degradable antimony nanostructure according to claim 2, which is characterized in that first described in step 1)
Dispersion liquid specific steps are as follows:
280-300mg sodium borohydride is dissolved in 25mL n-methyl-2-pyrrolidone, ultrasonic disperse 30min places it in oil
In bath, it is stirred continuously until that temperature rises to 60 DEG C, obtains the first dispersion liquid.
4. the preparation method of degradable antimony nanostructure according to claim 2, which is characterized in that second point of the step 2)
Dispersion liquid specific steps are as follows:
First 3.5-4.0g antimony chloride ultrasound is dissolved in 5mL n-methyl-2-pyrrolidone, is then quickly adding into the first dispersion
In liquid, it is uniformly mixed, persistently stirs 2h, centrifuge washing is scattered in again in 10mL deionized water, obtains the second dispersion liquid.
5. the preparation method of degradable antimony nanostructure according to claim 2, which is characterized in that the step 3) third point
Dispersion liquid specific steps are as follows:
8-8.1mg DOX is dissolved in 4mL deionized water, is uniformly mixed with the second dispersion liquid, after being protected from light and being stirred overnight, centrifugation
Washing, then be scattered in ultrapure water again, obtain third dispersion liquid.
6. the preparation method of degradable antimony nanostructure according to claim 2, which is characterized in that the step 4) it is specific
Step are as follows:
It is dissolved in 10mL deionized water by the surface organic ligands polyacrylic acid of 50-60mg, after ultrasonic disperse, disperses with third
Liquid is thoroughly mixed, and centrifuge washing is to get to for cooperateing with the degradable antimony nanostructure of chemotherapy and photo-thermal physical therapy.
7. the preparation method of degradable antimony nanostructure according to claim 2, which is characterized in that water-soluble in the step 4)
The surface organic ligands of property include: polyacrylic acid, polyvinylpyrrolidone, polyethylene glycol, polyethyleneimine.
8. degradable antimony nanostructure according to claim 1, which is characterized in that as diagnosis and treatment preparation, be applied to association
With chemotherapy and photo-thermal physical therapy.
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| CN110938423A (en) * | 2019-08-28 | 2020-03-31 | 上海大学 | Degradable antimony-coated rare earth up-conversion nano composite structure, preparation method and application |
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