CN109959783A - A kind of sample introduction adhesive tape and preparation method thereof - Google Patents
A kind of sample introduction adhesive tape and preparation method thereof Download PDFInfo
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- CN109959783A CN109959783A CN201910256949.0A CN201910256949A CN109959783A CN 109959783 A CN109959783 A CN 109959783A CN 201910256949 A CN201910256949 A CN 201910256949A CN 109959783 A CN109959783 A CN 109959783A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
- C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
- C08F220/10—Esters
- C08F220/12—Esters of monohydric alcohols or phenols
- C08F220/16—Esters of monohydric alcohols or phenols of phenols or of alcohols containing two or more carbon atoms
- C08F220/18—Esters of monohydric alcohols or phenols of phenols or of alcohols containing two or more carbon atoms with acrylic or methacrylic acids
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09J—ADHESIVES; NON-MECHANICAL ASPECTS OF ADHESIVE PROCESSES IN GENERAL; ADHESIVE PROCESSES NOT PROVIDED FOR ELSEWHERE; USE OF MATERIALS AS ADHESIVES
- C09J133/00—Adhesives based on homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Adhesives based on derivatives of such polymers
- C09J133/04—Homopolymers or copolymers of esters
- C09J133/06—Homopolymers or copolymers of esters of esters containing only carbon, hydrogen and oxygen, the oxygen atom being present only as part of the carboxyl radical
- C09J133/062—Copolymers with monomers not covered by C09J133/06
- C09J133/066—Copolymers with monomers not covered by C09J133/06 containing -OH groups
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
- C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
- C08F220/10—Esters
- C08F220/12—Esters of monohydric alcohols or phenols
- C08F220/16—Esters of monohydric alcohols or phenols of phenols or of alcohols containing two or more carbon atoms
- C08F220/18—Esters of monohydric alcohols or phenols of phenols or of alcohols containing two or more carbon atoms with acrylic or methacrylic acids
- C08F220/1808—C8-(meth)acrylate, e.g. isooctyl (meth)acrylate or 2-ethylhexyl (meth)acrylate
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- Polymers & Plastics (AREA)
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Abstract
The present invention relates to a kind of sample introduction adhesive tapes and preparation method thereof, wherein sample introduction adhesive tape includes the substrate layer being successively bonded, glue-line, nonwoven layer and buffering solution layer, the glue-line is the special acrylic acid ester polymer for being resistant to acid-base solution, and the buffer solution layer includes at least one of phosphate, carbonate, borate, organic acid, Tris buffer solution.The preparation method of sample introduction adhesive tape is the following steps are included: glue-line is coated on substrate layer, nonwoven layer is bonded after drying, buffer solution is used into coating machine dry 5~10min at 80~100 DEG C after nonwoven layer carries out dip coated, obtains required sample introduction adhesive tape.Compared with the prior art, the advantages of the present invention are as follows: there is reliable and stable sample introduction effect, reduce client manufacturing procedure, be easy to implement automated production.
Description
Technical field
The present invention relates to immunochromatography technique field, a kind of sample introduction adhesive tape and preparation method thereof is referred specifically to.
Background technique
In-vitro diagnosis (in vitro diagnosis, IVD), refers to except human body, passes through the sample (blood to human body
Liquid, body fluid, tissue etc.) detected and obtained the products & services of clinical diagnosis information.In-vitro diagnosis product is widely used in
Clinical each stage through tentative diagnosis, therapeutic scheme selection, efficiency evaluation, makes a definite diagnosis the full mistakes of disease treatments such as healing
Journey.Currently, 80% or so of clinical diagnosis information comes from in-vitro diagnosis, referred to as " eyes of doctor ".
The classification method of in-vitro diagnosis is relatively more, and according to testing principle or detection method, in-vitro diagnosis can be divided into life
Change diagnosis, immunodiagnosis, molecular diagnosis, bedside diagnosis, blood testing, cell detection, microorganism detection, urine detection, blood coagulation
The multiple types such as class diagnosis, fluidic cell diagnosis.Currently, biochemical, immune and molecular diagnosis agents are that China's diagnostic reagent is main
Three categories kind.
Test paper based on immunochromatography technique, composition include sample introduction pad/conjugate release pad/chromatographic film/absorption pad/bottom
Plate, such as patent of invention " the fluorescence immunoassay layer that number of patent application is CN201810491770.9 (notification number CN108918865A)
Analysis test strips and reagent card " disclose a kind of fluorescence immune chromatography test paper bar, including bottom plate, on bottom plate along its length sequentially
It is provided with sample pad, fluorescence pad, nitrocellulose filter and sample suction pad；Sample pad is Chong Die with fluorescence pad part, fluorescence pad and nitric acid
Cellulose membrane partly overlaps, and a side of fluorescence pad is located at the lower section of sample pad, and opposite another side of fluorescence pad is located at
The top of a side of nitrocellulose filter；Nitrocellulose filter partly overlaps with sample suction pad, and nitrocellulose filter is opposite
Another side be located at the lower section of sample suction pad；Detection line and nature controlling line are provided on nitrocellulose filter, and detection line is close to glimmering
Light pad；Sample pad is handled using sample pad treatment fluid, and the concentration in sample pad treatment fluid containing 0.1%~0.5% is 10%
Bromocresol green ethanol solution, detection sensitivity with higher, and detection efficiency and accuracy rate are higher.
Sample introduction part in test paper including above-mentioned fluorescence immune chromatography test paper bar in addition to chromatographic film is general using tradition
Prepared by technique, play the transmitting effect of liquid in entire test process, and avoids having an impact liquid.Traditional handicraft is big
Mostly each component substep handled by biological reagent is fit together again after bottom plate fitting glue film or double faced adhesive tape, or
Biological reagent processing is carried out to each component after fitting, mostly piece is bonded piece, and process is many and diverse and processing cost is high.
Summary of the invention
First technical problem to be solved by this invention is the status for the prior art, and it is steady to provide a kind of sample introduction effect
It is fixed reliable, manufacturing procedure is simple, is easy to implement the sample introduction adhesive tape of automated production.
Second technical problem to be solved by this invention is to provide a kind of preparation method of above-mentioned sample introduction adhesive tape.
The present invention solves technical solution used by first technical problem are as follows: a kind of sample introduction adhesive tape, it is characterised in that: packet
The substrate layer being successively bonded, glue-line, nonwoven layer and buffering solution layer are included, the glue-line is the extraordinary propylene for being resistant to acid-base solution
Acid ester polymer, the buffer solution layer include phosphate, carbonate, borate, organic acid, in Tris buffer solution at least
It is a kind of.
It preferably, further include surfactant in the buffer solution.
The present invention solves technical solution used by second technical problem are as follows: the preparation method of above-mentioned sample introduction adhesive tape,
It is characterized in that the following steps are included: glue-line is coated on substrate layer, is bonded nonwoven layer after dry, by buffer solution using applying
Cloth machine dry 5~10min at 80~100 DEG C after nonwoven layer carries out dip coated, obtains required sample introduction adhesive tape.
Preferably, the glue-line is made of following raw material and its mass fraction:
100 mass parts of acrylate polymer
0.01~0.5 mass parts of crosslinking agent.
Further, the glue-line preparation method the following steps are included:
(1) by 60~80 parts of Isooctyl acrylate monomer, the butyl acrylate of 10~30 mass parts, 0.5~3 mass parts third
Olefin(e) acid, the hydroxy-ethyl acrylate of 0.5~2 mass parts, the long chain acrylic ester monomer of 0.5~5 mass parts, 0.05~1.0 mass
The molecular weight regulator of part is mixed with the ethyl acetate of 50~100 mass parts, is warming up to 70 after stirring 1hr under nitrogen protection
DEG C, it is gradually added the initiator for reaction 4hr of 0.01~0.5 mass parts, adds the ethyl acetate dilution drop of 50~80 mass parts
Temperature obtains required acrylate polymer；
(2) acrylate polymer that step (1) obtains is mixed with crosslinking agent by mass fraction proportion, is obtained required
Glue-line.
Further, the weight average molecular weight of step (1) described acrylate polymer be 60~100W, solid content be 35~
45%, viscosity is 6000~10000cps.
Further,
The long chain acrylic ester monomer is at least one of lauryl acrylate, octadecyl ester, for increasing
Add water-fast alcohol resistance；
The molecular-weight adjusting is at least one of lauryl mercaptan, methyl styrene linear dimerization body, due to molten
It is easy to react too fast without containing benzene class in agent, molecular weight distribution is too wide, the controllable reaction rhythm of molecular weight regulator；
The initiator is azo-bis-iso-dimethyl, azodiisobutyronitrile, azobisisoheptonitrile, isopropylbenzene peroxidating
At least one of hydrogen, tert-butyl hydroperoxide；
The crosslinking agent is at least one of epoxy crosslinking agent, metallo-chelate.
Preferably, the nonwoven layer is made of following raw material and its mass percent:
Viscous fibre 60~80%
Polyester fiber 15~35%
Sea-island fibre 5~10%.
Further,
It is described viscous fine for viscose glue cotton-type short fiber, viscose glue wool type staple fiber, strong viscose rayon, polynosic, high curling
At least one of koplon, modified viscose fiber；Viscose rayon due to living through repeatedly in the fabrication process
Physical and chemical reaction causes the cracking of cellulose macromolecule group, its macromolecular is made to shorten, and molecular gap is larger, arrangement also loose zero
Disorderly, hydroxyl (- OH) polarity in molecule, great amount of hydroxy group (- OH) a large amount of adsorbed water molecules of meeting contained or other molecules,
It is allowed to provide good hygroscopicity；
The polyester fiber is dacron fibre, poly fiber, gathers to benzene two
Formic acid propylene diester fiber, poly- two ester fiber of 2,6- naphthalene diacid second, is modified and gathers polycyclohexane terephthalate fiber
At least one of ester group fiber；For polyester fiber because its fiber macromolecular chain rigidity is larger, elasticity modulus is high, and fiber is not variable
Shape, the fabric intensity with higher and elastic recovery capability of processing, and chemical resistance is good；
The sea-island fibre is water-soluble modified polyester/polyamide compound fibre；Sea-island fibre fabric not only has synthesis
Fiber it is non-ironing, good stability of the dimension and washing shrinkage are small the features such as, it is excellent also to have good, moisture absorption moisture-inhibiting of natural fiber drapability etc. concurrently
Point, and large specific surface area between sea-island fibre, gap is more, can assign fabric good filterability.
Further, the preparation method of the nonwoven layer is the following steps are included: with weighing machine by viscous fibre, polyester fiber, sea
Island fiber matches crawl by weight percent, carries out fiber winding, Alkali reduction method fibrillation by water jet process, and deionized water is rinsed,
Finally dry winding, obtains required nonwoven layer.
Further, the grammes per square metre of layer of cloth is 40~80g/m2, water absorption is 300~600g/m2, rate of water absorption is 1~3s/
35mm, tensile strength is in 2~5Kg/15mm, ion concentration < 5000ppm.
Compared with the prior art, the advantages of the present invention are as follows: a kind of integrally formed sample introduction adhesive tape is provided, including successively
Substrate layer, glue-line, nonwoven layer and the buffering solution layer of fitting, the sample introduction adhesive tape have certain filter effect to liquid is tested,
And can provide a stable pH environment, there is reliable and stable sample introduction effect, sample volume and the control of feed liquor time are certain
Range can cast out the use of sample suction pad in the design of certain test strips, reduce client manufacturing procedure, be easy to implement automation
Production.
Specific embodiment
Present invention is further described in detail with reference to embodiments.
Embodiment 1:
(1) by 80 parts of Isooctyl acrylate monomer, the butyl acrylate of 10 mass parts, the acrylic acid of 3 mass parts, 1.5 mass
Part hydroxy-ethyl acrylate, the lauryl acrylate of 3 mass parts, the lauryl mercaptan of 0.05 mass parts and 100 mass parts
Ethyl acetate mixing, is warming up to 70 DEG C after stirring 1hr under nitrogen protection, is gradually added the azodiisobutyronitrile of 0.05 mass parts
4hr is reacted, the ethyl acetate dilution cooling of 70 mass parts is added, obtains weight average molecular weight 80W, solid content 37%, viscosity is
The acrylate polymer of 8000cps；
(2) the viscous fibre of 80 mass parts, 15 mass parts polyester fibers, 5 mass of water-soluble modified poly esters/poly- are grabbed with weighing machine
Amide composite fibre carries out fiber winding, Alkali reduction method fibrillation by water jet process, and deionized water is rinsed, finally dry winding；?
It is 70g/m to grammes per square metre2, tensile strength 3.3Kg/15mm, spunlace non-woven cloth of the ion concentration less than 5000ppm；
(3) above-mentioned acrylate polymer 100g is taken, 0.025g E-100C crosslinking agent is added, is uniformly mixed, passes through scraper
Formula coating apparatus is applied in the PET base material layer with a thickness of 175 microns, and rubberization thickness is 30 microns, is bonded nonwoven layer after dry；
(4) pass through scraper type coating apparatus for the nonwoven cloth cover dip coated Tris-HCl buffer solution of above-mentioned composite material
(pH 8.5), dry 10min, obtains the sample introduction adhesive tape finally handled through buffer solution at 90 DEG C.
Embodiment 2:
(1) by 60 parts of Isooctyl acrylate monomer, the butyl acrylate of 30 mass parts, the acrylic acid of 0.5 mass parts, 2 mass
Part hydroxy-ethyl acrylate, the octadecyl esters of 5 mass parts, 1.0 mass parts methyl styrene linear dimerization body and 50
The ethyl acetate of mass parts mixes, and is warming up to 70 DEG C after stirring 1hr under nitrogen protection, is gradually added the azo of 0.12 mass parts
Two isobutyl dimethyl phthalates react 4hr, add the ethyl acetate dilution cooling of 80 mass parts, obtain weight average molecular weight 60W, admittedly contain
Amount 45%, viscosity are the acrylate polymer of 10000cps；
(2) the viscous fibre of 60 mass parts, 35 mass parts polyester fibers, 5 mass of water-soluble modified poly esters/poly- are grabbed with weighing machine
Amide composite fibre carries out fiber winding, Alkali reduction method fibrillation by water jet process, and deionized water is rinsed, finally dry winding；?
It is 40g/m to grammes per square metre2, tensile strength 2.1Kg/15mm, spunlace non-woven cloth of the ion concentration less than 5000ppm；
(3) above-mentioned acrylate polymer 100g is taken, 0.1g acetylacetone,2,4-pentanedione aluminum crosslinker is added, is uniformly mixed, by scraping
Blade coating apparatus is applied on the PS substrate layer with a thickness of 500 microns, and rubberization thickness is 35 microns, is bonded non-woven fabrics after dry
Layer；
(4) pass through scraper type coating apparatus for the nonwoven cloth cover dip coated Tris-HCl buffer solution of above-mentioned composite material
(pH8.5), the dry 8min at 95 DEG C, obtains the sample introduction adhesive tape finally handled through buffer solution.
Embodiment 3:
(1) by 70 parts of Isooctyl acrylate monomer, the butyl acrylate of 20 mass parts, the acrylic acid of 2 mass parts, 0.5 mass
The lauryl mercaptan agent and 70 mass parts of the hydroxy-ethyl acrylate of part, the lauryl acrylate of 0.5 mass parts, 0.5 mass parts
Ethyl acetate mixing, be warming up to 70 DEG C after stirring 1hr under nitrogen protection, be gradually added azo two different heptan of 0.01 mass parts
Nitrile reacts 4hr, adds the ethyl acetate dilution cooling of 80 mass parts, obtains weight average molecular weight 70W, solid content 38%, viscosity
For the acrylate polymer of 7000cps；
(2) viscous fine with weighing machine 70 mass parts of crawl, 20 mass parts polyester fibers, 10 mass of water-soluble modified poly esters/
Polyamide compound fibre carries out fiber winding, Alkali reduction method fibrillation by water jet process, and deionized water is rinsed, finally dry winding；
Obtaining grammes per square metre is 60g/m2, tensile strength 2.3Kg/15mm, spunlace non-woven cloth of the ion concentration less than 5000ppm；
(3) above-mentioned acrylate polymer 100g is taken, 0.04g E-AX crosslinking agent is added, is uniformly mixed, passes through scraper type
Coating apparatus is applied in the PET base material layer with a thickness of 50 microns, and rubberization thickness is 15 microns, is bonded nonwoven layer after dry；
(4) pass through scraper type coating apparatus for nonwoven cloth cover dip coated phosphate sodium dihydrogen-phosphorus of above-mentioned composite material
Sour disodium hydrogen buffer solution (pH6.5), dry 5min, obtains the sample introduction adhesive tape finally handled through buffer solution at 100 DEG C.
Embodiment 4:
(1) by 75 parts of Isooctyl acrylate monomer, the butyl acrylate of 15 mass parts, the acrylic acid of 1.5 mass parts, 1 mass
The hydroxy-ethyl acrylate, the octadecyl ester of 2.5 mass parts, the lauryl mercaptan of 0.3 mass parts and 100 mass parts of part
Ethyl acetate mixing, under nitrogen protection stir 1hr after be warming up to 70 DEG C, be gradually added two isobutyl of azo of 0.2 mass parts
Dimethyl phthalate reacts 4hr, adds the ethyl acetate dilution cooling of 80 mass parts, obtains weight average molecular weight 100W, solid content
35%, viscosity is the acrylate polymer of 6000cps；
(2) the viscous fibre of 60 mass parts, 33 mass parts polyester fibers, 7 mass of water-soluble modified poly esters/poly- are grabbed with weighing machine
Amide composite fibre carries out fiber winding, Alkali reduction method fibrillation by water jet process, and deionized water is rinsed, finally dry winding；?
It is 80g/m to grammes per square metre2, tensile strength 4.5Kg/15mm, spunlace non-woven cloth of the ion concentration less than 5000ppm；
(3) above-mentioned acrylate polymer 100g is taken, 0.3g acetylacetone,2,4-pentanedione aluminum crosslinker is added, is uniformly mixed, by scraping
Blade coating apparatus is applied in the PET base material layer with a thickness of 200 microns, and rubberization thickness is 25 microns, is bonded non-woven fabrics after dry
Layer；
(4) pass through scraper type coating apparatus for nonwoven cloth cover dip coated phosphate sodium dihydrogen-phosphorus of above-mentioned composite material
Sour disodium hydrogen buffer solution (pH6.5), dry 7min, obtains the sample introduction adhesive tape finally handled through buffer solution at 90 DEG C.
Embodiment 5:
(1) by 70 parts of Isooctyl acrylate monomer, the butyl acrylate of 20 mass parts, the acrylic acid of 2 mass parts, 1.2 mass
The hydroxy-ethyl acrylate, the long chain acrylic ester monomer of 2 mass parts, the lauryl mercaptan of 0.2 mass parts and 100 mass parts of part
Ethyl acetate mixing, under nitrogen protection stir 1hr after be warming up to 70 DEG C, be gradually added the tert-butyl isopropyl of 0.1 mass parts
Phenyl peroxide reactions 4hr adds the ethyl acetate dilution cooling of 50 mass parts, obtains weight average molecular weight 80W, solid content
39%, viscosity is the acrylate polymer of 8000cps；
(2) the viscous fibre of 70 mass parts, 25 mass parts polyester fibers, 5 mass of water-soluble modified poly esters/poly- are grabbed with weighing machine
Amide composite fibre carries out fiber winding, Alkali reduction method fibrillation by water jet process, and deionized water is rinsed, finally dry winding；?
It is 70g/m to grammes per square metre2, tensile strength 2.7Kg/15mm, spunlace non-woven cloth of the ion concentration less than 5000ppm；
(3) above-mentioned acrylate polymer 100g is taken, 0.01g E-100C crosslinking agent is added, is uniformly mixed, passes through scraper
Formula coating apparatus is applied on the PVC base layer with a thickness of 250 microns, and rubberization thickness is 25 microns, is bonded nonwoven layer after dry；
(4) pass through scraper type coating apparatus for nonwoven cloth cover dip coated boric acid-borate buffer solution of above-mentioned composite material
(pH7.8), the dry 7min at 95 DEG C, obtains the sample introduction adhesive tape finally handled through buffer solution.
Embodiment 6:
(1) by 60 parts of Isooctyl acrylate monomer, the butyl acrylate of 10 mass parts, the acrylic acid of 1.5 mass parts, 1 mass
Part hydroxy-ethyl acrylate, the lauryl acrylate of 1 mass parts, the lauryl mercaptan of 0.1 mass parts and 80 mass parts second
Acetoacetic ester mixing, is warming up to 70 DEG C after stirring 1hr under nitrogen protection, is gradually added the tert-butyl hydroperoxide of 0.5 mass parts
4hr is reacted, adds the ethyl acetate dilution cooling of 50 mass parts, obtaining weight average molecular weight is 90W, solid content 36%, viscosity
For the acrylate polymer of 7000cps；
(2) the viscous fibre of 70 mass parts, 25 mass parts polyester fibers, 7 mass of water-soluble modified poly esters/poly- are grabbed with weighing machine
Amide composite fibre carries out fiber winding, Alkali reduction method fibrillation by water jet process, and deionized water is rinsed, finally dry winding；?
It is 60g/m to grammes per square metre2, tensile strength 3.0Kg/15mm, spunlace non-woven cloth of the ion concentration less than 5000ppm；
(3) above-mentioned acrylate polymer 100g is taken, 0.5g acetylacetone copper crosslinking agent is added, is uniformly mixed, by scraping
Blade coating apparatus is applied in the PET base material layer with a thickness of 175 microns, and rubberization thickness is 50 microns, is bonded non-woven fabrics after dry
Layer；
(4) pass through scraper type coating apparatus for nonwoven cloth cover dip coated citric acid-sodium citrate of above-mentioned composite material
Buffer (pH6.6), dry 9min, obtains the sample introduction adhesive tape finally handled through buffer solution at 85 DEG C.
Embodiment 7:
(1) by 80 parts of Isooctyl acrylate monomer, the butyl acrylate of 10 mass parts, the acrylic acid of 0.5 mass parts, 2 mass
Part hydroxy-ethyl acrylate, 1.5 mass parts octadecyl ester, the methyl styrene linear dimerization body of 0.7 mass parts with
The ethyl acetate of 80 mass parts mixes, and is warming up to 70 DEG C after stirring 1hr under nitrogen protection, is gradually added the idol of 0.3 mass parts
Two isobutyl dimethyl phthalate initiator for reaction 4hr of nitrogen adds the ethyl acetate dilution cooling of 80 mass parts, obtains weight average molecular weight
90W, solid content 37%, acrylate polymer of the viscosity in 7000cps；
(2) viscous fine with weighing machine 60 mass parts of crawl, 30 mass parts polyester fibers, 10 mass of water-soluble modified poly esters/
Polyamide compound fibre carries out fiber winding, Alkali reduction method fibrillation by water jet process, and deionized water is rinsed, finally dry winding；
Obtaining grammes per square metre is 50g/m2, tensile strength 2.7Kg/15mm, spunlace non-woven cloth of the ion concentration less than 5000ppm；
(3) above-mentioned acrylate polymer 100g is taken, 0.05g acetylacetone,2,4-pentanedione aluminum crosslinker is added, is uniformly mixed, by scraping
Blade coating apparatus is applied on the PC substrate layer with a thickness of 200 microns, and rubberization thickness is 25 microns, is bonded non-woven fabrics after dry
Layer；
(4) pass through scraper type coating apparatus for the nonwoven cloth cover dip coated Tris-HCl buffer solution of above-mentioned composite material
(pH8.0), the dry 10min at 90 DEG C, obtains the sample introduction adhesive tape finally handled through buffer solution.
Embodiment 8:
(1) by 70 parts of Isooctyl acrylate monomer, the butyl acrylate of 20 mass parts, the acrylic acid of 2 mass parts, 1.5 mass
Part hydroxy-ethyl acrylate, the octadecyl esters of 2 mass parts, 0.3 mass parts methyl styrene linear dimerization body and 70
The ethyl acetate of mass parts mixes, and is warming up to 70 DEG C after stirring 1hr under nitrogen protection, is gradually added the azo of 0.1 mass parts
Two isobutyl dimethyl phthalate initiator for reaction 4hr add the ethyl acetate dilution cooling of 80 mass parts, obtain weight average molecular weight
80W, solid content 39%, the acrylate polymer of viscosity 8000cps.
(2) the viscous fibre of 65 mass parts, 30 mass parts polyester fibers, 5 mass of water-soluble modified poly esters/poly- are grabbed with weighing machine
Amide composite fibre carries out fiber winding, Alkali reduction method fibrillation by water jet process, and deionized water is rinsed, finally dry winding；?
It is 60g/m to grammes per square metre2, tensile strength 3.5Kg/15mm, spunlace non-woven cloth of the ion concentration less than 5000ppm；
(3) above-mentioned acrylate polymer 100g is taken, 0.25g acetylacetone,2,4-pentanedione aluminum crosslinker is added, is uniformly mixed, by scraping
Blade coating apparatus is applied on the PMMA substrate layer with a thickness of 200 microns, and rubberization thickness is 30 microns, is bonded non-woven fabrics after dry
Layer；
(4) pass through scraper type coating apparatus for the nonwoven cloth cover dip coated PBS buffer solution of above-mentioned composite material
(pH7.2), the dry 8min at 85 DEG C, obtains the sample introduction adhesive tape finally handled through buffer solution.
Comparative example 1:
(1) by 20 parts of Isooctyl acrylate monomer, the butyl acrylate of 70 mass parts, the acrylic acid of 5 mass parts and 50 mass
The ethyl acetate mixing of part, is warming up to 75 DEG C after stirring 1hr under nitrogen protection, the azo two for being gradually added 0.15 mass parts is different
Butyronitrile initiator for reaction 4hr adds the ethyl acetate dilution cooling of 80 mass parts, obtains weight average molecular weight 45W, solid content
43%, the acrylate polymer of viscosity 12000cps；
(2) the viscous fibre of 65 mass parts, 30 mass parts polyester fibers, 5 mass of water-soluble modified poly esters/poly- are grabbed with weighing machine
Amide composite fibre carries out fiber winding, Alkali reduction method fibrillation by water jet process, and deionized water is rinsed, finally dry winding；?
It is 50g/m to grammes per square metre2, tensile strength 2.4Kg/15mm；Ion concentration is less than the spunlace non-woven cloth of 5000ppm；
(3) above-mentioned acrylate polymer 100g is taken, 1.2g polymethylene polyphenyl polyisocyanate crosslinking agent, mixing is
It is even, it is applied in the PET base material layer with a thickness of 175 microns by scraper type coating apparatus, rubberization thickness is 25 microns, is pasted after dry
Close nonwoven layer；
(4) pass through scraper type coating apparatus for the nonwoven cloth cover dip coated Tris buffer solution of above-mentioned composite material
(pH8.5), the dry 8min at 85 DEG C, obtains the sample introduction adhesive tape finally handled through buffer solution.
Comparative example 2:
(1) by 80 parts of Isooctyl acrylate monomer, the butyl acrylate of 10 mass parts, the acrylic acid of 3 mass parts, 1.5 mass
Part hydroxy-ethyl acrylate, the lauryl acrylate of 3 mass parts, the lauryl mercaptan of 0.05 mass parts and 100 mass parts
Ethyl acetate mixing, is warming up to 70 DEG C after stirring 1hr under nitrogen protection, is gradually added the tert-butyl cumyl peroxide of 0.05 mass parts
Base peroxide initiator react 4hr, then plus 70 mass parts ethyl acetate dilute cooling, obtain weight average molecular weight 80W, admittedly contain
Amount 37%, the acrylate polymer of viscosity 8000cps；
(2) 100 mass parts polypropylene are taken, melting extrusion stretches to form long filament after be laid with networking, then it is solid by hot rolling mode
It is fixed, last splitting winding；Obtaining grammes per square metre is 60g/m2, tensile strength is the spun-bonded non-woven fabrics of 2.7Kg/15mm；
(3) above-mentioned acrylate polymer 100g is taken, 0.025g acetylacetone,2,4-pentanedione aluminum crosslinker is added, is uniformly mixed, passes through
Scraper type coating apparatus is applied on the PS substrate layer with a thickness of 300 microns, and rubberization thickness is 35 microns, is bonded non-woven fabrics after dry
Layer；Obtain final sample introduction adhesive tape.
Above-mentioned all embodiments and the performance test results of comparative example are as shown in table 1.
Note: in table 1, the test method of adhesive tape properties is as follows:
(1) under standard environment, 25 microns of PET adhesion strength: will be coated on the mixed acrylate copolymer of curing agent
On substrate, the adhesive tape of formation cuts into 25mm wide, and the sample of 150mm long is attached on polishing steel plate, with 2kg rubber axis
It rolls 3 times back and forth, after placing 20min, by cupping machine with 180 ° of the speed removings of 300mm/min；
(2) water absorption: being cut into 6cm*5cm specification for sample, places it under the conditions of constant temperature and humidity r for 24 hours, reaches damping
Test is fully saturated with increased weight when deionized water after balance；
(3) diffusion velocity: sample is cut into the circle that diameter is 35mm, r for 24 hours is placed it under the conditions of constant temperature and humidity, reaches
Its time needed completely by deionized water wetting under single hole no pressure mode is tested after to damping balance；
(4) coefficient of variation: after being combined in complex with the NC film for being attached with antibody into early pregnancy test strips, HCG standard specimen is used
Chemiluminescence immunoassay is tested, and is carried out the coefficient of variation (CV) value for test value and is calculated, it may be assumed that and the coefficient of variation (CV)=
Standard deviation (σ)/average value (μ) * 100%.Due to the typically no specific numerical value of immunochromatographic method, CV value can not be calculated, because
This is tested with chemiluminescence immunoassay.
Seen from table 1:
(1) acrylate polymer in Examples 1 to 8 after the processing of soda acid buffer solution, protect by adhesion strength performance
It holds and works as with preliminary phase, and there is no hydrolysis or cohesional failures；And since acrylic acid content is more in monomer and does not have in comparative example 1
Have using long chain acrylic ester monomer and molecular weight regulator, reaction is caused to generate polymer intolerant to acid-base buffer；
(2) sample introduction adhesive tape water absorption and diffusion rate are better than comparative example 1~2, more conducively sample introduction in Examples 1 to 8, compare
Non-woven fabric component ratio is different in example 1, and viscous fibre content is low, water suction and diffusion decline；In comparative example 2, used is poly- third
Alkene material hot rolling technology, water imbibition are poor；
(3) by the recipe verification of Examples 1 to 8, in following ranges (60~80 parts of Isooctyl acrylate monomer, 10~
The butyl acrylate of 30 mass parts, the acrylic acid of 0.5~3 mass parts, the hydroxy-ethyl acrylate of 0.5~2 mass parts, 0.5~5 matter
Measure the long chain acrylic ester monomer of part, the molecular weight regulator of 0.05~1.0 mass parts, the ethyl acetate of 50~100 mass parts
Mixing) adjust main monomer, long chain acrylic ester monomer, initiator, solvent and curing agent amount, can obtain buffer processing
Less, the coefficient of variation is lesser as a result, therefore, sample introduction adhesive tape of the invention has reliable and stable sample introduction for the variation of front and back adhesion strength
Effect.
The performance test results of table 1 all embodiments and comparative example
Claims (10)
1. a kind of sample introduction adhesive tape, it is characterised in that: including be successively bonded substrate layer, glue-line, nonwoven layer and buffering solution layer,
The glue-line is the special acrylic acid ester polymer for being resistant to acid-base solution, and the buffer solution layer includes phosphate, carbonate, boron
At least one of hydrochlorate, organic acid, Tris buffer solution.
2. a kind of preparation method of sample introduction adhesive tape according to claim 1, it is characterised in that the following steps are included: by glue-line
It is coated on substrate layer, is bonded nonwoven layer after dry, buffer solution is subjected to dip coated in nonwoven layer using coating machine
Dry 5~10min at 80~100 DEG C afterwards, obtains required sample introduction adhesive tape.
3. preparation method according to claim 2, which is characterized in that the glue-line is by following raw material and its mass parts array
At:
100 mass parts of acrylate polymer
0.01~0.5 mass parts of crosslinking agent
4. preparation method according to claim 3, which is characterized in that the preparation method of the glue-line the following steps are included:
(1) by 60~80 parts of Isooctyl acrylate monomer, the butyl acrylate of 10~30 mass parts, 0.5~3 mass parts propylene
Acid, the hydroxy-ethyl acrylate of 0.5~2 mass parts, the long chain acrylic ester monomer of 0.5~5 mass parts, 0.05~1.0 mass parts
Molecular weight regulator mixed with the ethyl acetate of 50~100 mass parts, under nitrogen protection stir 1hr after be warming up to 70 DEG C,
It is gradually added the initiator for reaction 4hr of 0.01~0.5 mass parts, adds the ethyl acetate dilution cooling of 50~80 mass parts,
Obtain required acrylate polymer；
(2) acrylate polymer that step (1) obtains is mixed with crosslinking agent by mass fraction proportion, obtains required glue
Layer.
5. the preparation method according to claim 4, it is characterised in that: the weight of step (1) described acrylate polymer is equal
Molecular weight is 60~100W, and solid content is 35~45%, and viscosity is 6000~10000cps.
6. the preparation method according to claim 4, it is characterised in that:
The long chain acrylic ester monomer is at least one of lauryl acrylate, octadecyl ester；
The molecular-weight adjusting is at least one of lauryl mercaptan, methyl styrene linear dimerization body；
The initiator be azo-bis-iso-dimethyl, azodiisobutyronitrile, azobisisoheptonitrile, isopropyl benzene hydroperoxide,
At least one of tert-butyl hydroperoxide；
The crosslinking agent is at least one of epoxy crosslinking agent, metallo-chelate.
7. preparation method according to claim 2, which is characterized in that the nonwoven layer is by following raw material and its quality hundred
Score composition:
Viscous fibre 60~80%
Polyester fiber 15~35%
Sea-island fibre 5~10%.
8. preparation method according to claim 7, it is characterised in that:
It is described viscous fine for viscose glue cotton-type short fiber, viscose glue wool type staple fiber, strong viscose rayon, polynosic, high curling high humidity
At least one of modulus viscose rayon, modified viscose fiber；
The polyester fiber is dacron fibre, poly fiber, poly terephthalic acid
Propylene diester fiber, polycyclohexane terephthalate fiber, poly- two ester fiber of 2,6- naphthalene diacid second, modified poly ester base
At least one of fiber；
The sea-island fibre is water-soluble modified polyester/polyamide compound fibre.
9. according to the described in any item preparation methods of claim 7~8, which is characterized in that the preparation method of the nonwoven layer
The following steps are included: viscous fibre, polyester fiber, sea-island fibre are matched crawl by weight percent with weighing machine, by water jet process
Fiber winding, Alkali reduction method fibrillation are carried out, deionized water is rinsed, and finally dry winding, obtains required nonwoven layer.
10. according to the described in any item preparation methods of claim 7~8, it is characterised in that: the grammes per square metre of the nonwoven layer is 40
~80g/m2, water absorption is 300~600g/m2, rate of water absorption is 1~3s/35mm, and tensile strength is in 2~5Kg/15mm, ion
Content < 5000ppm.
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| CN110564309A (en) * | 2019-09-16 | 2019-12-13 | 新纶科技（常州）有限公司 | solvent-free acrylate adhesive composition and preparation method of ultrathin foam adhesive tape thereof |
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