CN109959610A - A kind of microfluidic liquid sample pool tested and analyzed for dynamic spectrum and its purposes - Google Patents
A kind of microfluidic liquid sample pool tested and analyzed for dynamic spectrum and its purposes Download PDFInfo
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- CN109959610A CN109959610A CN201711433465.6A CN201711433465A CN109959610A CN 109959610 A CN109959610 A CN 109959610A CN 201711433465 A CN201711433465 A CN 201711433465A CN 109959610 A CN109959610 A CN 109959610A
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- 239000007788 liquid Substances 0.000 title claims abstract description 42
- 238000001228 spectrum Methods 0.000 title claims abstract description 25
- 230000006698 induction Effects 0.000 claims abstract description 11
- 239000004809 Teflon Substances 0.000 claims abstract description 4
- 229920006362 Teflon® Polymers 0.000 claims abstract description 4
- 230000005540 biological transmission Effects 0.000 claims description 9
- 238000006243 chemical reaction Methods 0.000 claims description 6
- 238000012360 testing method Methods 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 230000003595 spectral effect Effects 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000011835 investigation Methods 0.000 claims description 2
- 238000005259 measurement Methods 0.000 abstract description 10
- 239000012530 fluid Substances 0.000 abstract description 8
- 230000003287 optical effect Effects 0.000 abstract description 8
- 238000013461 design Methods 0.000 abstract description 4
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- 238000004140 cleaning Methods 0.000 abstract description 2
- 230000007797 corrosion Effects 0.000 abstract description 2
- 238000005260 corrosion Methods 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 154
- 238000001514 detection method Methods 0.000 description 15
- 238000000862 absorption spectrum Methods 0.000 description 8
- -1 polytetrafluoroethylene Polymers 0.000 description 8
- 230000001052 transient effect Effects 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 6
- 239000004810 polytetrafluoroethylene Substances 0.000 description 6
- HVQAJTFOCKOKIN-UHFFFAOYSA-N flavonol Natural products O1C2=CC=CC=C2C(=O)C(O)=C1C1=CC=CC=C1 HVQAJTFOCKOKIN-UHFFFAOYSA-N 0.000 description 5
- 235000011957 flavonols Nutrition 0.000 description 5
- 229960004194 lidocaine Drugs 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- 238000005086 pumping Methods 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 238000001069 Raman spectroscopy Methods 0.000 description 3
- 238000001237 Raman spectrum Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000010183 spectrum analysis Methods 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 230000001788 irregular Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 239000010453 quartz Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- NTRKEYLXTPNPIY-UHFFFAOYSA-N 2-[4-(diethylamino)phenyl]-3-hydroxychromen-4-one Chemical compound C1=CC(N(CC)CC)=CC=C1C1=C(O)C(=O)C2=CC=CC=C2O1 NTRKEYLXTPNPIY-UHFFFAOYSA-N 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
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- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000001427 coherent effect Effects 0.000 description 1
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- 238000007796 conventional method Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
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- 239000012454 non-polar solvent Substances 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Optical Measuring Cells (AREA)
Abstract
The invention discloses a kind of microfluidic liquid sample pool tested and analyzed for dynamic spectrum and its purposes, and the microfluidic sample cell includes magnetic induction rotation system, motor drive and control system and sample cell system.The sample cell designs simple, compact-sized, using flexible and occupied space very little, is suitable for being placed in the optical path of instrument；The sample cell is since the sample containment space of restriction is small, is suitable for carrying out optical measurement to micro-example；Good airproof performance, anticorrosive property, the measurement suitable for volatile high corrosion sample；The sample cell may make all samples smooth flow in the accommodation space that it is limited, and there is no fluid stagnation areas, avoid or reduce signal noise caused by sample turbulent flow；The structure that rotor uses teflon-coated designs, and while avoiding rotor by sample etches, also avoids rotor contaminated samples；For convenience detach and cleaning, the influence to light path of optical spectrometer is very small, convenient for measurement is repeated several times.
Description
Technical field
The present invention relates to spectral detections to analyze used instrument and equipment technical field, more particularly to a kind of for dynamic optical
Compose the microfluidic liquid sample pool tested and analyzed and its purposes.
Background technique
In order to obtain the chemical bond or functional group's information of organic molecule, it is often necessary to carry out the measurement of spectrum to it.And
For fluid sample, traditional detection method is that fluid sample is made to film as defined in meeting and carries out light again using liquid-film method
The measurement of spectrum, but liquid-film method is only applicable to non-volatile sample, due to the nonpolar solvent contact angle of non-volatile sample
It is too small, it is not easy to form liquid film, it is difficult to satisfactory film be made；And due to manually-operated uncertainty, obtained knot
Fruit application condition is big, is often only used for qualitative analysis.
A kind of flowing sample cell is disclosed in the prior art, i.e., sample is successively inputted to sample cell by pipeline and pump
In cuvette, and the flowing of sample is kept, to acquire dynamic spectrum.But for highly viscous fluid, due to spectral signal
There are certain hysteresis qualitys for acquisition, and need biggish sample volume, when causing using such flowing sample cell, test
Sensitivity and precision are lower；Moreover, the sample cell of large capacity is not suitable for valuable rare sample yet.
When sample volume to be analyzed is smaller, such as 5 microlitres or less volume, conventional sample cell then have limitation
Property.Light and the interaction for being contained in analyte in sample cell are depended on across the amount of the light of sample cell projection.How to keep high
While sensitivity, the size of sample cell is reduced, is a challenge to accommodate minimal amount of sample volume.In addition, preparing corpusculum
Long-pending sample cell is there is also being difficult to fill and be easy to generate bubble, thus the problems such as influencing optical measurement and analysis.Especially needle
To laser spectral analysis, in the case where high energy laser irradiates for a long time, to keep the stability of test result, need sample continuous
Ground flowing, and the sample cell of small size is more difficult to realize.In short, currently used sample cell needs to consider that following three is big
Restricted problem: (1) influence of the solvent to reaction must be taken into consideration and to spectrometric interference；(2) it needs to examine in quantitative determination
Consider the configuration process of sample solution and its error of introducing；(3) temperature also needs to consider that compared with relative superiority or inferiority, solvent volatilization or its steam produce
Raw high pressure harm.
Summary of the invention
In order to overcome the prior art carrying out present in laser spectrum measurement to liquid sample, stability is poor, structure is multiple
Miscellaneous, the problems such as being difficult to ensure vulnerable to external disturbance, measurement accuracy, the present invention provide it is a kind of tested and analyzed for dynamic spectrum it is micro-
Working fluid sample cell and its purposes.Microfluidic liquid sample pool of the invention, compact-sized, using flexible and be that one kind can be held
A variety of homogeneous and heterogeneous reaction the dynamic spectrum sample cells of row.Not only may be directly applied to current mainstream ultraviolet spectrometer,
Visible spectrophotometer and infrared spectrometer, it may also be used for Raman spectrometer further expands the application range of spectrum analysis.
For the limitation of existing dynamic spectrum acquisition device, sample cell of the present invention mainly considers following restricted problem: (1)
How solvent influence to reaction is overcome and to spectrometric interference；(2) need to consider sample solution in quantitative determination
Configuration process and its error of introducing；(3) also need to consider solvent volatilization or the high pressure harm of its steam generation when temperature is higher；
(4) for micro-example, while how keeping high sensitivity, the size of sample cell is reduced, to accommodate minimal amount of sample body
Product；(5) in the case where high energy laser irradiates for a long time, the stability and consistency of test result to be kept.
The present invention to achieve the above object, using following technical scheme:
A kind of microfluidic liquid sample pool tested and analyzed for dynamic spectrum comprising magnetic induction rotation system, motor
Transmission and control system and sample cell system；
The sample cell system includes sample cell；
The magnetic induction rotation system includes rotor, magnetic turntable and magnetic shaft；The rotor is placed in sample cell；It is described
Magnetic turntable is used to control the rotation for the rotor being placed in sample cell；
The motor drive and control system include power supply, controller and direct current generator；The power supply is with controller and directly
Galvanic electricity mechatronics；The controller is used to control the revolving speed of direct current generator；
The direct current generator is connect with the magnetic shaft, and the direct current generator is for driving magnetic shaft to rotate；The magnetic turns
Axis is connect with the magnetic turntable, and the magnetic turntable is positioned close at sample cell.
According to the present invention, the sample cell includes by the pond body of translucent material (such as quartz) preparation, and pond body top is equipped with band
The opening of sealing cover, the pond body bottom surface, front, the back side and side are tightly connected, and the pond body front and the back side are light transmission
Face, the pond body top are sample test window, and pond body lower part is rotor stirring area.
According to the present invention, the light path of the sample cell is less than or equal to 1mm；I.e. light is less than by the distance of the sample cell
Equal to 1mm.
In the present invention, the shape of the sample cell can be rule, be also possible to it is irregular, for example, cuboid,
Square or polymorphic structure, but the light path of the sample cell, i.e. light are less than or equal to 1mm by the distance of the sample cell；
Well known to a person skilled in the art if the shape of the sample cell is cuboid, the width of the sample cell defined is practical
Pass through the distance of the sample cell for light, also the light path of the as described sample cell.
According to the present invention, the rotor is selected from the rotor that diameter is less than or equal to 0.5mm.Preferably, the rotor is selected from straight
The rotor of teflon-coated of the diameter less than or equal to 0.5mm.The polytetrafluoroethylene (PTFE) clad structure can be effectively prevented to be checked
Rotor is corroded in organic solvent dissolution and strong acid in sample, is also possible to prevent rotor by sample etches.
According to the present invention, the sample cell system further includes sample cell slot, the first screw and fastening gasket；The sample cell
The side side wall of U-shaped sample cell slot is arranged in the U-shaped structure of slot, first screw, is fixed sample cell by fastening gasket
In sample cell slot.
According to the present invention, the microfluidic liquid sample pool further includes cantilever, and the magnetic shaft is fixed by the second screw
On cantilever top；The direct current generator is fixed on jib foot by third screw.
According to the present invention, second screw is fixedly connected by cantilever shalving with cantilever.
According to the present invention, the motor drive and control system further include speed changer, and the direct current generator passes through transmission shaft
It is connected with speed changer, the direct current generator passes through the rotation of transmission control magnetic shaft.Preferably, the gear selecting is from electronic
Machine or engine, for example, micromotor or tiny engine.
According to the present invention, the revolving speed of the magnetic shaft is at 5-500 revolutions per seconds.
In the present invention, the motor drive and control system are for driving and controlling the magnetic induction rotation system.Magnetic strength
The magnetic field for answering the rotation of magnetic turntable in rotation system to form rotation is placed in sample cell system under the induction of rotating excitation field
Rotor rotated with given pace, so that stirring to sample in sample cell system is realized, so that forming miniflow in fluid sample
Rotating ring border.
According to the present invention, the microfluidic liquid sample pool further includes the first support system, the first support system packet
The first adjusting bracket, the first locating slot and first base are included, one end of first adjusting bracket is connect with the first locating slot, and described
The other end of one adjusting bracket is connect with first base；First locating slot includes groove compatible with sample cell slot, described
Sample cell slot and the first locating slot in vertical direction can plug cooperations.
According to the present invention, first adjusting bracket includes first sleeve, the first bolt and the first frame body, the first sleeve
It is set in the first frame body, and first sleeve is fixed in the first frame body by the first bolt.First sleeve moves up and down
The height of the first locating slot above adjustable first adjusting bracket, and then the height of sample cell is adjusted, and pass through the first bolt
It is fixed in the first frame body.
According to the present invention, first support system further includes first point for being positioned close to the first adjusting bracket bottom sidewall
Li card adjuster and third micrometer adjuster, the first micrometer adjuster and the third micrometer adjuster are vertically set
It sets, is respectively used to regulate and control the first adjusting bracket in the horizontal and vertical directions.
According to the present invention, the microfluidic liquid sample pool further includes the second support system, the second support system packet
The second adjusting bracket, the second locating slot and second base are included, one end of second adjusting bracket is connect with the second locating slot, and described
The other end of two adjusting brackets is connect with second base；Second locating slot includes groove compatible with cantilever, the cantilever
It in vertical direction can plug cooperation with the second locating slot.
According to the present invention, second adjusting bracket includes second sleeve, the second bolt and the second frame body, the second sleeve
It is set in the second frame body, and second sleeve is fixed in the second frame body by the second bolt.Second sleeve moves up and down
The height of the second locating slot above adjustable second adjusting bracket, and then the height of magnetic turntable and magnetic shaft is adjusted, and pass through
Second bolt is fixed in the second frame body.
According to the present invention, second support system further includes second point for being positioned close to the second adjusting bracket bottom sidewall
Li card adjuster and the 4th micrometer adjuster, the second micrometer adjuster and the 4th micrometer adjuster are vertically set
It sets, is respectively used to regulate and control the second adjusting bracket in the horizontal and vertical directions.
In the present invention, the height of first adjusting bracket and the second adjusting bracket not only adjustable sample cell and cantilever, also
Its fine tuning in the horizontal direction may be implemented.
The present invention also provides the purposes of the above-mentioned microfluidic liquid sample pool tested and analyzed for dynamic spectrum, are used as more
Homogeneous and heterogeneous reaction dynamic spectrum sample cell is planted, or is used for femtosecond spectral investigation.
Beneficial effects of the present invention:
(1) the microfluidic liquid sample pool design tested and analyzed for dynamic spectrum of the invention is simple, compact-sized, makes
It is suitable for being placed in the optical path of instrument with flexible and occupied space very little.
(2) the microfluidic liquid sample pool tested and analyzed for dynamic spectrum of the invention is empty since the sample of restriction accommodates
Between it is small, be suitable for micro-example carry out optical measurement；Good airproof performance, anticorrosive property, the survey suitable for volatile high corrosion sample
Amount；
(3) the microfluidic liquid sample pool tested and analyzed for dynamic spectrum of the invention may make all samples in its limit
Smooth flow in fixed accommodation space, there is no fluid stagnation areas, avoid or reduce signal noise caused by sample turbulent flow；
The structure that rotor uses teflon-coated designs, and while avoiding rotor by sample etches, also avoids rotor dirt
Contaminate sample；For convenience detach and cleaning, the influence to light path of optical spectrometer is very small, convenient for measurement is repeated several times.
(4) the microfluidic liquid sample pool tested and analyzed for dynamic spectrum of the invention is suitable as a variety of homogeneous and non-
The dynamic spectrum sample cell of homogeneous reaction, not only may be directly applied to the ultraviolet of current mainstream, visible light and infrared spectrometer,
It can also be used in the spectrometer device of the complexity such as Raman spectrometer, further expand the application range of spectrum analysis.
Detailed description of the invention
Fig. 1 is the overall construction drawing of microfluidic liquid sample pool of the invention.
Fig. 2 is the structure chart of a preferred embodiment of microfluidic liquid sample pool of the invention.
Fig. 3 is the structure chart of a preferred embodiment of microfluidic liquid sample pool of the invention.
Fig. 4 is the structure chart of a preferred embodiment of microfluidic liquid sample pool of the invention.
Fig. 5 is lignocaine flavonol femtosecond Broadband pump-detection transient absorption spectra and pumping shown in embodiment 2
(pump)-excitation (push)/loses money in business the multiple-pulse control transient absorption spectra figure of (dump)-detection (probe).
Fig. 6 is lignocaine flavonol femtosecond Broadband pump-detection transient absorption spectra and pumping shown in embodiment 2
(pump)-excitation (push)/loses money in business multiple-pulse control transient absorption spectra the moving at 560nm of (dump)-detection (probe)
Force diagram figure.
Fig. 7 is the real-time acquisition interface figure of femtosecond stimlated Raman spectrum shown in embodiment 3.
Fig. 8 is that femtosecond shown in embodiment 4 pumps (pump)-excitation (the push)/eclipse of the sun or moon (dump)-detection (probe) light
Spectra system dynamic analysis surface chart.
Appended drawing reference in Fig. 1-Fig. 4 is as follows:
1- sample cell, 2- fastening gasket, the first screw of 3-, 4- rotor, 5- sample cell slot, the first frame body of 6-, the first spiral shell of 7-
Bolt, 8- first sleeve, the first adjusting bracket of 9-, 10- the first micrometer adjuster, 11- first base, 12- magnetic turntable, 13- magnetic turn
Axis, 14- cantilever shalving, the second screw of 15-, 16- cantilever, 17- speed changer, 18- direct current generator, 19- transmission shaft, the second frame body of 20-,
21- second sleeve, the second bolt of 22-, the second adjusting bracket of 23-, 24- the second micrometer adjuster, 25- second base, 26- third
Micrometer adjuster, the 4th micrometer adjuster of 27-, 28- power supply, 29- controller.
Specific embodiment
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.Furthermore, it is to be understood that after having read content disclosed in this invention, ability
Field technique personnel can make various changes or modifications the present invention, and such equivalent forms equally fall within protection defined by the present invention
Within the scope of.
Experimental method used in following embodiments is conventional method unless otherwise specified；Institute in following embodiments
Reagent, material etc., are commercially available unless otherwise specified.
In the description of the present invention, it should be noted that term " first ", " second ", " third " and " the 4th " etc. are only used
In description purpose, and relative importance is not indicated that or implies.
Embodiment 1
Below with reference to Fig. 1-Fig. 4 and example in detail a specific embodiment of the invention, as Figure 1-Figure 4, this reality
It applies example and a kind of microfluidic liquid sample pool tested and analyzed for dynamic spectrum is provided comprising magnetic induction rotation system, motor
Transmission and control system and sample cell system；
The sample cell system includes sample cell 1；
The magnetic induction rotation system includes rotor 4, magnetic turntable 12 and magnetic shaft 13；The rotor 4 is placed on sample cell 1
In；The magnetic turntable 12 is used to control the rotation for the rotor 4 being placed in sample cell 1；
The motor drive and control system include power supply 28, controller 29 and direct current generator 18；The power supply 28 and control
Device 29 and direct current generator 18 electrical connection processed；The controller 29 is used to control the revolving speed of direct current generator 18；
The direct current generator 18 is connect with the magnetic shaft 13, and the direct current generator 18 is for driving magnetic shaft 13 to rotate；
The magnetic shaft 13 is connect with the magnetic turntable 12, and the magnetic turntable 12 is positioned close at sample cell 1.
In a preferred embodiment of invention, the sample cell 1 includes by the pond of translucent material (such as quartz) preparation
Body, pond body top are equipped with the opening of with closure, and the pond body bottom surface, front, the back side and side are tightly connected, and the pond body is just
Face and the back side are light-transmissive surface, and the pond body top is sample test window, and pond body lower part is rotor stirring area.The sample cell
Light path be less than or equal to 1mm, i.e., light is by the distance of the sample cell less than or equal to 1mm.In the present invention, the sample cell
Shape can be rule, be also possible to irregular, for example, cuboid, square or polymorphic structure, but the sample
The light path in pond, i.e. light are less than or equal to 1mm by the distance of the sample cell；Well known to a person skilled in the art if the sample
The shape in product pond is cuboid, then the practical width of the sample cell defined is distance of the light by the sample cell, namely
For the light path of the sample cell.
In a preferred embodiment of invention, the rotor 4 is selected from the rotor that diameter is less than or equal to 0.5mm.
In a preferred embodiment of invention, the rotor 4 is selected from the polytetrafluoroethylene (PTFE) that diameter is less than or equal to 0.5mm
The rotor of package.The dissolution of the organic solvent in sample to be tested and strong acid can be effectively prevented in the polytetrafluoroethylene (PTFE) clad structure
Corrode rotor, is also possible to prevent rotor by sample etches.
In a preferred embodiment of the present invention, the sample cell system further includes sample cell slot 5, the first screw 3
And fastening gasket 2；The side side wall of U-shaped sample cell slot 5 is arranged in the U-shaped structure of the sample cell slot 5, first screw 3,
Sample cell 1 is fixed in sample cell slot 5 by fastening gasket 2.
In a preferred embodiment of the present invention, the microfluidic liquid sample pool further includes cantilever 16, the magnetic
Shaft 13 is fixed on 16 top of cantilever by the second screw 15；The direct current generator 18 is fixed on by third screw (not shown)
16 lower part of cantilever.
In a preferred embodiment of the present invention, second screw 15 connects by the way that cantilever shalving 14 and cantilever 16 are fixed
It connects.
In a preferred embodiment of the present invention, the motor drive and control system further include speed changer 17, institute
It states direct current generator 18 to be connected by transmission shaft 19 with speed changer 17, the direct current generator 18 controls magnetic shaft 13 by speed changer 17
Rotation.The revolving speed of the magnetic shaft 13 is at 5-500 revolutions per seconds.The speed changer 17 is selected from motor or engine, for example, micro-
Type motor or tiny engine.
In the present invention, the motor drive and control system are for driving and controlling the magnetic induction rotation system.Magnetic strength
The magnetic field for answering the rotation of the magnetic turntable 12 in rotation system to form rotation is placed on sample cell system under the induction of rotating excitation field
Rotor 4 in system is rotated with given pace, so that the stirring to the sample in sample cell system is realized, so that shape in fluid sample
At microfluidic environment.
In a preferred embodiment of the present invention, the microfluidic liquid sample pool further includes the first support system,
First support system includes the first adjusting bracket 9, the first locating slot and first base 11, one end of first adjusting bracket 9
It is connect with the first locating slot, the other end of first adjusting bracket 9 is connect with first base 11；First locating slot include with
The compatible groove of sample cell slot 5, the sample cell slot 5 and the first locating slot in vertical direction can plug cooperations.
In a preferred embodiment of the present invention, first adjusting bracket 9 includes first sleeve 8,7 and of the first bolt
First frame body 6, the first sleeve 8 are set in the first frame body 6, and first sleeve 8 is fixed on first by the first bolt 7
In frame body 6.The height of the first locating slot for moving up and down adjustable first adjusting bracket, 9 top of first sleeve 8, and then adjust
The height of sample cell 1, and be fixed in the first frame body 6 by the first bolt 7.
In a preferred embodiment of the present invention, first support system further includes being positioned close to the first adjusting
The the first micrometer adjuster 10 and third micrometer adjuster 26 of 9 bottom sidewall of frame, 10 He of the first micrometer adjuster
The third micrometer adjuster 26 is vertically arranged, and is respectively used to regulate and control the first adjusting bracket 9 in the horizontal and vertical directions.
In a preferred embodiment of the present invention, the microfluidic liquid sample pool further includes the second support system,
Second support system include the second adjusting bracket 23, the second locating slot and second base 25, the one of second adjusting bracket 23
End is connect with the second locating slot, and the other end of second adjusting bracket 23 is connect with second base 25；The second locating slot packet
Groove compatible with cantilever 16 is included, the cantilever 16 and the second locating slot in vertical direction can plug cooperations.
In a preferred embodiment of the present invention, second adjusting bracket 23 includes second sleeve 21, the second bolt
22 and second frame body 20, the second sleeve 21 be set in the second frame body 20, and second sleeve 21 is consolidated by the second bolt
It is scheduled in the second frame body 20.The height for moving up and down the second locating slot above adjustable second adjusting bracket of second sleeve 21
Degree, and then the height of magnetic turntable 12 and magnetic shaft 13 is adjusted, and be fixed in the second frame body 20 by the second bolt 22.
In a preferred embodiment of the present invention, second support system further includes being positioned close to the second adjusting
Second micrometer adjuster 24 of 23 bottom sidewall of frame and the 4th micrometer adjuster 27,24 He of the second micrometer adjuster
The 4th micrometer adjuster 27 is vertically arranged, and is respectively used to regulate and control the second adjusting bracket 23 in the horizontal and vertical directions.
In a preferred embodiment of the present invention, first adjusting bracket 9 and the second adjusting bracket 23 can not only be adjusted
The height for saving sample cell and cantilever, can also realize its fine tuning in the horizontal direction.
Embodiment 2
Microfluidic liquid sample pool described in embodiment 1 can be used in femtosecond Broadband pump (pump)-detection (probe) light
In spectrometer system, the more of the macroscopic view of phase complicated molecule and the Chemical Kinetics field on single molecules level can be condensed
Item research.
The sample for the microfluidic liquid sample pool detection that the present embodiment uses is lignocaine flavonol (4 '-N, N-
Diethylamino-3-hydroxyflavone, DEAHF).
In the exemplary application, the shape of the sample cell is cuboid, the specification of the sample cell be 1mm (width) ×
10mm (height) × 45mm (length), volume are less than 0.5ml, applicable wavelengths 200-2500nm.The practical width of the sample cell is light
By the distance of the sample cell, the also light path of the as described sample cell.
Fig. 5 is lignocaine flavonol femtosecond Broadband pump-detection transient absorption spectra and pumping shown in embodiment 2
(pump)-excitation (push)/loses money in business the multiple-pulse control transient absorption spectra figure of (dump)-detection (probe).
Fig. 6 is lignocaine flavonol femtosecond Broadband pump-detection transient absorption spectra and pumping shown in embodiment 2
(pump)-excitation (push)/loses money in business multiple-pulse control transient absorption spectra the moving at 560nm of (dump)-detection (probe)
Force diagram figure.
As can be known from Fig. 5 and Fig. 6, the sample cell of the application can be used for condensed phase supramolecular (conjugate light-emitting
Point in polymer molecule, super branched molecule and photosynthetic Light harvest antenna pigment molecular and organic/inorganic hybridization material system
Electric charge transfer and solvation process etc. in son) in chromophore between coherent electron and relevant exciton conduction kinetic characteristics
And the research that mechanism is probed into.
Embodiment 3
It is this increasingly complex that microfluidic liquid sample pool described in embodiment 1 can be used for femtosecond stimlated Raman spectrum instrument
In device (result is as shown in Figure 7), and achieve good effect.
In the exemplary application, the shape of the sample cell is cuboid, the specification of the sample cell be 1mm (width) ×
10mm (height) × 45mm (length), volume are less than 0.5ml, applicable wavelengths 200-2500nm.The practical width of the sample cell is light
By the distance of the sample cell, the also light path of the as described sample cell.
Fig. 7 is the real-time acquisition interface figure of femtosecond stimlated Raman spectrum shown in embodiment 3.As shown in Figure 7, it can be seen that right
In rare valuable micro-example, also it is able to maintain with the excited Raman signal of the microfluidic liquid sample pool system acquisition very high
Stability and sensitivity, for follow-up signal analysis provide good guarantee.
Embodiment 4
Microfluidic liquid sample pool described in embodiment 1 can be used for the femtosecond Broadband pump (pump)-of another complexity
It excites in (push)/eclipse of the sun or moon (dump)-detection (probe) spectrometer device, equally achieves good effect.
In the exemplary application, the shape of the sample cell is cuboid, the specification of the sample cell be 1mm (width) ×
10mm (height) × 45mm (length), volume are less than 0.5ml, applicable wavelengths 200-2500nm.The practical width of the sample cell is light
By the distance of the sample cell, the also light path of the as described sample cell.
Fig. 8 is that femtosecond shown in embodiment 4 pumps (pump)-excitation (the push)/eclipse of the sun or moon (dump)-detection (probe) light
Spectra system dynamic analysis surface chart.As can be seen from Figure 8, to faint double difference sub-signal, due to keeping the miniflow of sample
It is dynamic, while avoiding the spectrum generated by flowing disturbance, also maintain the smooth consistency of signal.
More than, embodiments of the present invention are illustrated.But the present invention is not limited to above embodiment.It is all
Within the spirit and principles in the present invention, any modification, equivalent substitution, improvement and etc. done should be included in guarantor of the invention
Within the scope of shield.
Claims (10)
1. a kind of microfluidic liquid sample pool tested and analyzed for dynamic spectrum comprising magnetic induction rotation system, motor pass
Dynamic and control system and sample cell system；
The sample cell system includes sample cell；
The magnetic induction rotation system includes rotor, magnetic turntable and magnetic shaft；The rotor is placed in sample cell；The magnetic turns
Disk is used to control the rotation for the rotor being placed in sample cell；
The motor drive and control system include power supply, controller and direct current generator；The power supply and controller and direct current
Mechatronics；The controller is used to control the revolving speed of direct current generator；
The direct current generator is connect with the magnetic shaft, and the direct current generator is for driving magnetic shaft to rotate；The magnetic shaft with
The magnetic turntable connection, and the magnetic turntable is positioned close at sample cell.
2. microfluidic liquid sample pool according to claim 1, which is characterized in that the sample cell includes by translucent material
The pond body of preparation, pond body top are equipped with the opening of with closure, and the pond body bottom surface, front, the back side and side are tightly connected, institute
It states pond body front and the back side is light-transmissive surface, the pond body top is sample test window, and pond body lower part is rotor stirring area.
Preferably, the light path of the sample cell is less than or equal to 1mm.
Preferably, the rotor is selected from the rotor that diameter is less than or equal to 0.5mm.
Preferably, the rotor is selected from the rotor of teflon-coated of the diameter less than or equal to 0.5mm.
3. microfluidic liquid sample pool according to claim 1 or 2, which is characterized in that the sample cell system further includes
Sample cell slot, the first screw and fastening gasket；The U-shaped structure of sample cell slot, first screw are arranged in U-shaped sample cell
Sample cell is fixed in sample cell slot by the side side wall of slot by fastening gasket.
4. microfluidic liquid sample pool according to claim 1 or 2, which is characterized in that the microfluidic liquid sample pool
It further include cantilever, the magnetic shaft is fixed on cantilever top by the second screw；The direct current generator is fixed by third screw
In jib foot.
5. microfluidic liquid sample pool according to claim 1, which is characterized in that the motor drive and control system are also
Including speed changer, the direct current generator is connected by transmission shaft with speed changer, and the direct current generator is turned by transmission control magnetic
The rotation of axis；The revolving speed of the magnetic shaft is at 5-500 revolutions per seconds.
6. microfluidic liquid sample pool according to claim 1, which is characterized in that the microfluidic liquid sample pool also wraps
The first support system is included, first support system includes the first adjusting bracket, the first locating slot and first base, and described first adjusts
One end of section frame is connect with the first locating slot, and the other end of first adjusting bracket is connect with first base；First positioning
Slot includes groove compatible with sample cell slot, and the sample cell slot and the first locating slot in vertical direction can plug cooperations.
7. microfluidic liquid sample pool according to claim 6, which is characterized in that first adjusting bracket includes first set
Cylinder, the first bolt and the first frame body, the first sleeve are set in the first frame body, and are consolidated first sleeve by the first bolt
It is scheduled in the first frame body.
8. microfluidic liquid sample pool according to claim 6, which is characterized in that first support system further includes setting
It sets in the first micrometer adjuster and third micrometer adjuster close to the first adjusting bracket bottom sidewall, first micrometer
Adjuster and the third micrometer adjuster are vertically arranged, and are respectively used to regulate and control the first adjusting in the horizontal and vertical directions
Frame.
9. microfluidic liquid sample pool according to claim 1 or 6, which is characterized in that the microfluidic liquid sample pool
It further include the second support system, second support system includes the second adjusting bracket, the second locating slot and second base, and described the
One end of two adjusting brackets is connect with the second locating slot, and the other end of second adjusting bracket is connect with second base；Described second
Locating slot includes groove compatible with cantilever, and the cantilever and the second locating slot in vertical direction can plug cooperations.
Preferably, second adjusting bracket includes second sleeve, the second bolt and the second frame body, and the second sleeve is set to
In two frame bodies, and second sleeve is fixed in the second frame body by the second bolt；
Second support system further includes the second micrometer adjuster and for being positioned close to the second adjusting bracket bottom sidewall
Four micrometer adjusters, the second micrometer adjuster and the 4th micrometer adjuster are vertically arranged, and are respectively used to
Regulate and control the second adjusting bracket on horizontal and vertical direction.
10. the purposes of the microfluidic liquid sample pool of any of claims 1-9 tested and analyzed for dynamic spectrum,
It is used as a variety of homogeneous and heterogeneous reaction dynamic spectrum sample cells, or is used for femtosecond spectral investigation.
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| CN105651585A (en) * | 2014-12-03 | 2016-06-08 | 天津港东科技发展股份有限公司 | Spectrometer magnetic stirring system |
| CN105935752A (en) * | 2016-07-08 | 2016-09-14 | 东北大学 | Vertical electromagnetic stirring method for controlling casting blank center quality |
| CN106290161A (en) * | 2016-09-08 | 2017-01-04 | 刘雳 | A kind of sample cell for Dynamic Absorption spectra collection |
| CN206235551U (en) * | 2016-11-14 | 2017-06-09 | 河南工业大学 | A kind of sample fixed support for spectral detection |
| CN207764109U (en) * | 2017-12-26 | 2018-08-24 | 中国科学院化学研究所 | A kind of microfluidic liquid sample pool for dynamic spectrum detection and analysis |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN201444157U (en) * | 2009-04-03 | 2010-04-28 | 泰安市科瑞光学仪器有限公司 | Full-automatic infrared oil tester |
| CN105651585A (en) * | 2014-12-03 | 2016-06-08 | 天津港东科技发展股份有限公司 | Spectrometer magnetic stirring system |
| CN105259358A (en) * | 2015-11-10 | 2016-01-20 | 中国科学院重庆绿色智能技术研究院 | Multi-channel rotary type Raman spectroscopy detection sample pool device and detecting method thereof |
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| CN109959610B (en) | 2024-07-16 |
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