CN109917036A - Gentamicin procaine ties up the HPLC-ELSD detection method of gentamicin C components content in B12 capsule - Google Patents

Abstract

The invention belongs to field of chemical detection, more particularly to the HPLC-ELSD detection method of gentamicin C components content in a kind of gentamicin procaine dimension B12 capsule, including (1) chromatographic condition and system suitability (2) solution prepare (3) measurement.This method is easy to operate, detection efficiency is high, error is small, the method of inspection is more stable, accuracy, precision, specificity, detection limit, durability of this method etc. meet methodology validation requirement, with very strong practicability, the content of gentamicin C components in gentamicin procaine dimension B12 capsule can be quickly detected, thus the effectively quality of control gentamicin procaine dimension B12 capsule.

Description

Gentamicin procaine ties up the HPLC- of gentamicin C components content in B12 capsule ELSD detection method

Technical field

The invention belongs to field of chemical detection, and in particular to celebrating is big mould in a kind of gentamicin procaine dimension B12 capsule The HPLC-ELSD detection method of plain component C content.

Background technique

Gentamicin procaine dimension B12 capsule (former name gastritis capsule also known as gentamicin procaine capsule, with The compound mouth being made of gentamicin sulphate, procaine hydrochloride, vitamin B12 and appropriate amount of auxiliary materials down referred to as " capsule ")) Formulation.Wherein, gentamicin sulphate is aminoglycoside antibiotics, to various gram-negative bacterias, gram-positive bacteria and Helicobacter pylori has antibacterial action, can inhibit helicobacter pylori, plays local bactericidal effect under one's belt；Hydrochloric acid Proca For local anaesthetics, sealing process can be played to the nerve endings on damaging mucosal surface, block the stimulation of lesion Central nervous system, from And rapid pain relief；Vitamin B12 is substance necessary to form alimentary canal normal epithelium cell.Three's cooperation can be played anti- Bacterium, analgesic and the effect for promoting gastric mucosal, are used clinically for alleviating acute and chronic gastritis symptom and anti-inflammatory, analgesic, promotion Stomach lining reparation.

Gentamicin procaine ties up B12 capsule current standard as the rising of " national drug standards " chemicals provincial standard National standard (the 6th) WS-10001-(HD-0524) -2002, State Food and Drug Administration standard YBH15222004 With State Food and Drug Administration standard YBH20972006, the detection in three parts of standards for gentamicin sulphate has The identification (WS-10001-(HD-0524) -2002 of sulfate), thin layer identify (YBH15222004, YBH20972006) and Assay, the Con trolling index without its component.Gentamicin sulphate is aminoglycoside antibiotics, is with gentamicinC₁、C_1a、 C₂、C_2aEtc. mixture based on components, no UV absorption.Main pharmacodynamics ingredient one of of the gentamicin sulphate as this product, mark Lack corresponding inspection item in standard, is a undoubtedly major hidden danger for the control of product quality.

Summary of the invention

The purpose of the present invention is in view of the shortcomings of the prior art and provide a kind of gentamicin procaine tie up B12 The HPLC-ELSD detection method of gentamicin C components content in capsule ties up B12 to gentamicin procaine using this method The content of gentamicin C components is measured in capsule, easy to operate, and detection efficiency is high, and error is small, can quickly detect to celebrate The content of big mycin component C, thus the effectively quality of control gentamicin procaine dimension B12 capsule.

To achieve the above object, the present invention adopts the following technical scheme that:

A kind of gentamicin procaine ties up the HPLC-ELSD detection method of gentamicin C components content in B12 capsule, including Following steps:

(1) chromatographic condition and system suitability:

It is filler, Shiseido ACR C18 4.6 × 250mm, 5 μm of chromatographic columns, with first with octadecylsilane chemically bonded silica Alcohol is mobile phase A, and 0.02mol/L trifluoroacetic acid is Mobile phase B, carries out gradient elution, flow velocity 1ml/min, column temperature 30 by table 1 DEG C, atomization temperature is 70 DEG C, air velocity 1.6SLM, and gentamicin standard solution chromatogram should be consistent with standard diagram；

(2) prepared by solution:

The preparation of gentamicin sulphate standard solution

Gentamicin sulphate standard items 100mg is taken, it is accurately weighed, it is placed in 10mL measuring bottle, scale is dissolved and be diluted to water, is shaken It is even, it is accurate respectively to measure 1,3,5mL, it is diluted with water to 10mL, is shaken up, concentration, which is made, respectively may be about 1mg/mL, 3mg/mL, 5mg/ The standard solution of mL is 1., 2., 3.；

The preparation of test solution:

It takes this product content to mix, weighs in right amount, be equivalent to average loading amount, accurately weighed, precision plus water 5mL, shaking make sulfuric acid Gentamicin dissolution, filtering take subsequent filtrate as test solution；

(3) it measures:

Precision measures standard solution, and 1., 2., 3. each 20 μ L according to above-mentioned chromatographic condition is injected separately into liquid chromatograph, records Chromatogram calculates the equation of linear regression of standard solution each component log concentration value and corresponding peak area logarithm, phase relation Number (r) is not less than 0.99；It takes test solution to be measured in the same method, calculates separately confession with the equation of linear regression of gentamicin each component Amount (the C of component is corresponded in test product_tcx), and calculate by following formula the content (%, u/u) of each component；

In formula, Cx is the content %, u/u of gentamicin each component；C_tcxContent for each component gone out by regression equation calculation mg/ml；It is averaged loading amount g for test sample；W is test sample sampling amount mg；V is test sample extension rate；Labelled amount is in terms of g.

C₁Should be labelled amount 15.3%~28.0%, C_1aIt should be 19.6%~48.0%, C of labelled amount_2a+C₂It should be mark The 28.5%~63.8% of amount, four component total contents are not less than labelled amount 85%.

The invention has the benefit that

The HPLC-ELSD inspection of gentamicin C components content in a kind of gentamicin procaine dimension B12 capsule provided by the invention Survey method, this method is easy to operate, and detection efficiency is high, and error is small, and the method for inspection is more stable, the accuracy of the method for the present invention, essence Density, specificity, detection limit, durability etc. meet methodology validation requirement, have very strong practicability, can quickly detect Gentamicin procaine ties up the content of gentamicin C components in B12 capsule out, to effectively control gentamicin Proca Because tieing up the quality of B12 capsule.

Detailed description of the invention

Fig. 1 is to tie up B12 capsule sample to 47 batch gentamicin procaines using detection method to be measured The four component total content distribution map of gentamicin sulphate obtained；

Fig. 2 is to be measured to obtain to 47 batch gentamicin procaines dimension B12 capsule sample using detection method Gentamicin sulphate C₁Constituent content distribution map；

Fig. 3 is to be measured to obtain to 47 batch gentamicin procaines dimension B12 capsule sample using detection method Gentamicin sulphate C_1aConstituent content distribution map；

Fig. 4 is to be measured to obtain to 47 batch gentamicin procaines dimension B12 capsule sample using detection method Gentamicin sulphate C₂+C_2aContent distribution figure；

Fig. 5 is to tie up B12 capsule sample to the gentamicin procaine that 4 enterprises produce using detection method to survey Surely the 4 enterprise's sample gentamicin sulphate total content average value comparison diagrams obtained；

Fig. 6 is to tie up B12 capsule sample to the gentamicin procaine that 4 enterprises produce using detection method to survey Surely the 4 enterprise sample gentamicin sulphate C obtained_1aConstituent content average value comparison diagram；

Fig. 7 is to tie up B12 capsule sample to the gentamicin procaine that 4 enterprises produce using detection method to survey Surely the 4 enterprise sample gentamicin sulphate C obtained₁Constituent content average value comparison diagram；

Fig. 8 is to tie up B12 capsule sample to the gentamicin procaine that 4 enterprises produce using detection method to survey Surely the 4 enterprise sample gentamicin sulphate C obtained₂+C_2aConstituent content average value comparison diagram；

Fig. 9 is that gentamicin procaine ties up gentamicin sulphate compound mensuration typical case's map in B12 capsule.

Specific embodiment

Embodiment 1

One, a kind of gentamicin procaine ties up the HPLC-ELSD detection method of gentamicin C components content in B12 capsule, packet Include following steps:

(1) chromatographic condition and system suitability:

It is filler, Shiseido ACR C18 4.6 × 250mm, 5 μm of chromatographic columns, with first with octadecylsilane chemically bonded silica Alcohol is mobile phase A, and 0.02mol/L trifluoroacetic acid is Mobile phase B, carries out gradient elution, flow velocity 1ml/min, column temperature 30 by table 1 DEG C, atomization temperature is 70 DEG C, air velocity 1.6SLM, and gentamicin standard solution chromatogram should be consistent with standard diagram；

(2) prepared by solution:

The preparation of gentamicin sulphate standard solution

Gentamicin sulphate standard items 100mg is taken, it is accurately weighed, it is placed in 10mL measuring bottle, scale is dissolved and be diluted to water, is shaken It is even, it is accurate respectively to measure 1,3,5mL, it is diluted with water to 10mL, is shaken up, concentration, which is made, respectively may be about 1mg/mL, 3mg/mL, 5mg/ The standard solution of mL is 1., 2., 3.；

The preparation of test solution:

It takes this product content to mix, weighs in right amount, be equivalent to average loading amount, accurately weighed, precision plus water 5mL, shaking make sulfuric acid Gentamicin dissolution, filtering take subsequent filtrate as test solution；

(3) it measures:

Precision measures standard solution, and 1., 2., 3. each 20 μ L according to above-mentioned chromatographic condition is injected separately into liquid chromatograph, records Chromatogram calculates the equation of linear regression of standard solution each component log concentration value and corresponding peak area logarithm, phase relation Number (r) is not less than 0.99；It takes test solution to be measured in the same method, calculates separately confession with the equation of linear regression of gentamicin each component Amount (the C of component is corresponded in test product_tcx), and calculate by following formula the content (%, u/u) of each component；

In formula, Cx is the content %, u/u of gentamicin each component；C_tcxContent for each component gone out by regression equation calculation mg/ml；It is averaged loading amount g for test sample；W is test sample sampling amount mg；V is test sample extension rate；Labelled amount is in terms of g.

Two, the HPLC-ELSD of gentamicin C components content is detected in gentamicin procaine dimension B12 capsule of the present invention The methodology validation situation of method is as follows:

1, linear relationship is investigated

Take gentamicin sulphate standard items (lot number 130326-201716, C1 content 15.9%, C1a content 15.2%, C2 content 16.9%, C2a content 10.3%；There is provided by National Institute for Food and Drugs Control) 200mg, it is accurately weighed, it is placed in 20mL measuring bottle In, scale is dissolved and be diluted to water, is shaken up, it is accurate respectively to measure 0.5,1,2.5,5,10mL, it is diluted with water to 10mL, is shaken It is even, be made concentration respectively may be about 0.5 μ g/mL, 1 μ g/mL, 2.5 μ g/mL, 5 μ g/mL, 10 μ g/mL linear work solution (1), (2), (3), (4), (5).Precision measures linear work solution (1), (2), (3), (4), (5) each 20 μ L, is injected separately into liquid phase color Spectrometer records chromatogram, carries out linear regression to corresponding peak area logarithm with each component log concentration value, the results are shown in Table 2.Knot Fruit shows that gentamicin each component linear relationship within the scope of respective concentration is good.

2, instrument sample introduction precision is investigated

Above-mentioned linear work solution (3) is taken, 5 needle of continuous sample introduction, the RSD of 4 components is respectively 2.4%, 3.4%, 0.6%, 2.4%, It the results are shown in Table 3.

3, stability of solution is investigated

Take (Haikou Qili Pharmaceutical Co., Ltd.'s production, lot number 171101) by the preparation of test solution in the method for the present invention Method carries out preparing test solution, detects under the chromatographic condition limited in the methods of the invention, as the result is shown each group in solution Point good in 12 hours internal stabilities, the RSD of tetra- components of C1a, C2, C2a, C1 is respectively 2.0%, 4.7%, 6.2%, 3.5%, knot Fruit is shown in Table 4.

4, the rate of recovery is investigated

Take gentamicin sulphate (production of North China Hua Luan Co., Ltd, pharmacy group, lot number Q160604) in right amount, it is accurately weighed, point It is not dissolved in water and standard solution L, M, H that concentration is about 0.5mg/mL, 1mg/mL, 1.5mg/mL is made；

The rate of recovery investigate solution L: take gentamicin procaine dimension B12 capsule (Haikou Qili Pharmaceutical Co., Ltd. production, Lot number 171101) content mixing, it weighs appropriate (being approximately equivalent to gentamicin sulphate 5mg), accurately weighed, 10mL is added in precision Standard solution L, shaking dissolve gentamicin sulphate, filter, take subsequent filtrate.

The rate of recovery investigates solution M: weighing content in right amount (being approximately equivalent to gentamicin sulphate 10mg), accurately weighed, essence Close addition 10mL standard solution M, shaking dissolve gentamicin sulphate, filter, take subsequent filtrate.

The rate of recovery investigates Solution H: weighing content in right amount (being approximately equivalent to gentamicin sulphate 15mg), accurately weighed, essence Close addition 10mL standard solution H, shaking dissolve gentamicin sulphate, filter, take subsequent filtrate.

The rate of recovery of each concentration investigates solution and prepares 3 parts in parallel, examines under the chromatographic condition limited in the methods of the invention It surveys, the results are shown in Table 5.The result shows that the rate of recovery is good, method is feasible.

5, repeatability is investigated

Take gentamicin procaine dimension B12 capsule (Haikou Qili Pharmaceutical Co., Ltd.'s production, lot number 171101) content Object is made repeatability by the preparation method of test solution in the method for the present invention and investigates 6 parts of solution, limits in the methods of the invention Chromatographic condition under detect, the results are shown in Table 6.Repeatability is good as the result is shown.

6, method detection limit and quantitative limit are investigated

Three, application effect

1, using the detection side HPLC-ELSD of gentamicin C components content in gentamicin procaine of the present invention dimension B12 capsule Method detects the 47 batch gentamicin procaines dimension B12 capsule sample that different enterprises produce, and measurement gentamicin is general In Lu Kayinwei B12 capsule the case where gentamicin C components content,

Instrument and reagent: 380 high performance liquid chromatograph of Aglient 1260- ELSD；Electronic balance METTLER XS205DU； Gentamicin sulphate standard items (lot number 130326-201716, C1 content 15.9%, C1a content 15.2%, C2 content 16.9%, C2a Content 10.3%；It is provided by National Institute for Food and Drugs Control).

The sulphur for only having Chinese Pharmacopoeia to record in domestic all gentamicin sulphate preparation relevant criterions prevailing for the time being in force at present Sour injection liquid of gentamicin has included gentamicin sulphate component check item, and provides: C₁Should be labelled amount 15.3%~ 28.0%, C_1aIt should be 19.6%~48.0%, C of labelled amount_2a+C₂It should be the 28.5%~63.8% of labelled amount, four components always contain Amount is not less than labelled amount 85%, therefore this result is evaluated in this, as reference pair.Measurement result is shown in Table 7.

2, sample measurement result is analyzed

Overall distribution situation: 47 batch sample total content average values 103.3%, content range 111.5%~93.9%；C1a content Average value 33.2%, content range 25.6%~39.6%；C1 content average value 22.2%, content range 19.9%~27.0%；C2+ As a result C2a content average value 47.9%, content range 42.7%~54.5% meet regulation, qualification rate 100%.47 batch samples Product total content and each component content distribution figure are shown in FIG. 1 to FIG. 4.

Data among enterprises compare: gentamicin sulphate 4 component total content Hainan day are bright to be up to 108.5%, Zhejiang Tianrui Minimum 97.1%；C₁It is trimly pharmacy 26.6%, minimum Haikou surprise power 21.8% that content is maximum；C_1aMaximum content is trimly Pharmacy 36.6%, minimum Zhejiang Tianrui 29.0%； C₂+C_2aContent maximum Hainan day bright 51.0%, minimum trimly pharmacy 43.1%；4 4 component total contents of enterprise's sample gentamicin sulphate are all larger than 85%, and each component content meets regulation.4 enterprise's samples are total Content average value and the comparison of each component content average value are shown in Table 8 and Fig. 5~Fig. 8.

3, gentamicin sulphate raw material components measurement result and analysis

Version Chinese Pharmacopoeia gentamicin sulphate gentamicin C components item regulation in 2015: C₁It should be 14%~22%, C_1aIt should be 10% ~23%, C₂+C_2aIt should be 17%~36%, four component total contents must not be lower than 50.0%.7 batch sulphur that 7 enterprises are provided Sour gentamicin raw material is measured, as the result is shown being produced by North China Hua Luan Co., Ltd, pharmacy group except the auspicious new offer in Zhejiang Gentamicin sulphate raw material C1 content and total content be below outside prescribed limit, other API measurement results meet regulation, examine Consider the sample that this sampling observation is not extracted into the auspicious new production in Zhejiang, and the raw material lot number provided is more early, however not excluded that enterprise does not give birth to for a long time Production is improper to surplus stock preservation to lead to the underproof situation of assay.Measurement result is shown in Table 9.

It is above-mentioned as it can be seen that tieing up gentamicin C components content in B12 capsule using gentamicin procaine of the present invention HPLC-ELSD detection method, easy to operate, detection efficiency is high, and error is small, and applicability is wide；It is raw to each enterprise using this method The gentamicin procaine dimension B12 capsule of production is detected, and can fast and accurately detect that gentamicin procaine is tieed up The content of gentamicin C components in B12 capsule, to be conducive to tie up B12 to the gentamicin procaine that each enterprise produces The quality of capsule sample is determined.

Claims (2)

1. the HPLC-ELSD detection method of gentamicin C components content in a kind of gentamicin procaine dimension B12 capsule, It is characterized in that, comprising the following steps:

(1) chromatographic condition and system suitability:

It is filler, Shiseido ACR C18 4.6 × 250mm, 5 μm of chromatographic columns, with first with octadecylsilane chemically bonded silica Alcohol is mobile phase A, and 0.02mol/L trifluoroacetic acid is Mobile phase B, carries out gradient elution, flow velocity 1ml/min, column temperature 30 by table 1 ℃；

(2) prepared by solution:

The preparation of gentamicin sulphate standard solution

Gentamicin sulphate standard items 100mg is taken, it is accurately weighed, it is placed in 10mL measuring bottle, scale is dissolved and be diluted to water, is shaken It is even, it is accurate respectively to measure 1,3,5mL, it is diluted with water to 10mL, is shaken up, concentration, which is made, respectively may be about 1mg/mL, 3mg/mL, 5mg/ The standard solution of mL is 1., 2., 3.；

The preparation of test solution:

It takes this product content to mix, weighs in right amount, be equivalent to average loading amount, accurately weighed, precision plus water 5mL, shaking make sulfuric acid Gentamicin dissolution, filtering take subsequent filtrate as test solution；

(3) it measures:

Precision measures standard solution, and 1., 2., 3. each 20 μ L according to above-mentioned chromatographic condition is injected separately into liquid chromatograph, records Chromatogram calculates the equation of linear regression of standard solution each component log concentration value and corresponding peak area logarithm, phase relation Number r is not less than 0.99；It takes test solution to be measured in the same method, is calculated separately with the equation of linear regression of gentamicin each component for examination The amount C of component is corresponded in product_tcx, and calculate by following formula the content Cx of each component；

In formula, Cx is the content %, u/u of gentamicin each component；C_tcxContent for each component gone out by regression equation calculation mg/ml；It is averaged loading amount g for test sample；W is test sample sampling amount mg；V is test sample extension rate；Labelled amount is in terms of g.

2. the HPLC- of gentamicin C components content in gentamicin procaine dimension B12 capsule according to claim 1 ELSD detection method, which is characterized in that in step (1) chromatographic condition and system suitability, atomization temperature is 70 DEG C, air-flow Speed is 1.6SLM.