CN109916901A - The method for quick identification of calm and peaceful black-bone chicken egg and hybridization black-bone chicken egg - Google Patents
The method for quick identification of calm and peaceful black-bone chicken egg and hybridization black-bone chicken egg Download PDFInfo
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Abstract
The invention discloses the method for quick identification of a kind of calm and peaceful black-bone chicken egg and hybridization black-bone chicken egg, comprising the following steps: Step 1: screening: filtering out egg size in numerous eggs to be detected in the black-bone chicken egg of 40.23 ± 3.6g, 44.54 ± 4.5g;Step 2: measurement: filtering out the transverse diameter and eggshell quality of egg in measuring process one;Step 3: identifying: being differentiated by discriminant equation formula: Y=1.160 × eggshell quality+8.922 × eggshell transverse diameter -39.494, wherein: Y value is then calm and peaceful black-bone chicken egg between -2.84 to -0.08, Y value is then hybridization black-bone chicken egg between 0.12 to 2.46, method of the invention only can need to effectively be identified by appearance index, easy to operate, quick, simple using equipment, easy to operate;Accuracy rate is up to 90% or more after being verified to eggshell using stereomicroscope observation.
Description
Technical field
The invention belongs to the true and false authentication technique field of agricultural product more particularly to a kind of calm and peaceful black-bone chicken egg with to hybridize black-bone chicken egg fast
Fast discrimination method.
Background technique
After calm and peaceful Gallus domesticlus brisson obtains national " Products of Local Geographical Indication certificate of protection ", Industrial agglomeration, shape are pushed forward comprehensively around Gallus domesticlus brisson
Processing is butchered at breeding production---- the specialty industries chain of Gallus domesticlus brisson food deep processing is delegated by Jiangxi saving of labor's letter and gives " Jiangxi Province
Calm and peaceful Gallus domesticlus brisson food industries base " title.By 2017,61 family of relevant food enterprise is settled, wherein the above enterprise of scale
21 families, main business income is up to 55.1 hundred million yuan.In 40 kilometers of radius centered on Taihe County, it is distributed with 106 and raises on a household basis and grow enterprise
Industry and professional raiser, it is more than annual 2000000 plumage of the Gallus domesticlus brisson amount of delivering for sale, 24,000,000 pieces of annual Gallus domesticlus brisson egg production or more.These cultivation
Householder will still use the small peasant economy mode for voluntarily purchasing medicine, voluntarily purchase material, voluntarily selling, and " unification supplies distance for medicine, uniformly
Material, uniformly for young, unified epidemic prevention, unified sale " mode there are also larger gaps, this is for the protection of provenance and the protection of epidemic situation
Control has the calm and peaceful Gallus domesticlus brisson of biggish potential risk to originate in Jiangxi Province, Wushan northern foot, Taihe County, also known as Wushan Chickens.
Egg is that people's acquisition animal derived protein matter is most straight as one of maximum animal-derived food of China's consumption of resident amount
The source connect." calm and peaceful black-bone chicken egg " keyword is searched for, and selects representative commodity according to Taobao's rule is integrated ordered.From
From the point of view of search result, single piece of calm and peaceful black-bone chicken egg highest unit price is 6.6 yuan/piece, and minimum unit price is 2.0 yuan/piece, and average unit price is
3.94 yuan/piece, highest price differential is up to 4.6 yuan.On the one hand it is the influence because of brand factor, produces in the crow of the calm and peaceful way Wang Po fowl industry
Egg has brand premium, ranks first from sales volume with time high price of 5.2 yuan/piece, illustrates calm and peaceful black-bone chicken egg to high-end consumer group
Body values " calm and peaceful Gallus domesticlus brisson food flagship store " with authoritative shop.There is no specific method to differentiate black-bone chicken egg in consumer true
When pseudo-, selecting such source area shop is the consumer behavior of rationality.
But at present about the data of calm and peaceful black-bone chicken egg it is existing in, do not provide about calm and peaceful black-bone chicken egg (purebred, hybridization)
The comprehensive data of middle nutritional ingredient and discrimination method, this brings huge puzzlement for consumer and market surpervision personnel, how full
Sufficient people avoid hybridization black-bone chicken egg from rushing the market of pure calm and peaceful black-bone chicken egg the sharp increase demand of the high-quality calm and peaceful black-bone chicken egg of orthodox school
It hits, the sound development protected and reinforce calm and peaceful Gallus domesticlus brisson industry has important theory and realistic meaning.
Therefore, by the more purebred calm and peaceful black-bone chicken egg of mass data with hybridize calm and peaceful black-bone chicken egg egg exterior quality, eggshell join
Several difference carries out comprehensive comparative analysis research, obtains fast and effectively volume discrimination method.And made by the comparison of nutritional ingredient
For evidence, determination rate of accuracy is greatly improved, carries out calm and peaceful purebred silkies strain protection of resources for system from now on and development and utilization mentions
For helpful reference and reference.
Summary of the invention
It is excellent it is an object of the invention to solve at least the above problems and/or defect, and provide at least to will be described later
Point.
The present invention provide the calm and peaceful black-bone chicken egg with hybridize black-bone chicken egg method for quick identification, it is easy to operate, accuracy rate is high,
Sample requirement is few.
In order to realize these purposes according to the present invention and other advantages, providing calm and peaceful black-bone chicken egg and hybridize black-bone chicken egg
Method for quick identification, comprising the following steps:
Step 1: screening: filtering out in numerous eggs to be detected egg size in 40.23 ± 3.6g, 44.54 ± 4.5g
Black-bone chicken egg;
Step 2: measurement: filtering out the transverse diameter and eggshell quality of egg in measuring process one;
Step 3: identifying: differentiated by discriminant equation formula:
Y=1.160 × eggshell quality+8.922 × eggshell transverse diameter -39.494
Wherein: Y value is then calm and peaceful black-bone chicken egg between -2.84 to -0.08, and Y value is then hybridization between 0.12 to 2.46
Black-bone chicken egg.
Preferably, the black-bone chicken egg that step 1 filters out is preliminary identification, and egg size is calm and peaceful Gallus domesticlus brisson in 40.23 ± 3.6g
Egg, egg size are hybridization black-bone chicken egg in 44.54 ± 4.5g.
Preferably, in step 2, the transverse diameter is the overall diameter of egg, by vernier caliper or miking,
It is accurate to 0.01mm.
Preferably, in step 1, when measuring egg size, need to egg be cleaned up and is dried, use balance or height
Precision platform balance is weighed, and 0.01g is accurate to.
Preferably, in step 2, eggshell does not include matter in egg and egg film, and eggshell is cleaned up and dried,
It is weighed using balance or high-precision platform balance, is accurate to 0.01g.
Calm and peaceful black-bone chicken egg of the present invention with hybridize black-bone chicken egg method for quick identification, greatly simplify purebred calm and peaceful Gallus domesticlus brisson
The differentiation means of egg.Method of the invention is simple, sample size is few, strong operability, accuracy rate are high, is the majority of consumers and market
The protection of the original seed of supervision and management person and calm and peaceful Gallus domesticlus brisson provides a kind of relatively simple quick discrimination method.
Further advantage, target and feature of the invention will be partially reflected by the following instructions, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
It obtains other drawings based on these drawings.
Fig. 1 is stereomicroscope observation eggshell outer surface figure;
Fig. 2 is stereomicroscope observation eggshell of the present invention outer surface figure;
Fig. 3 is stereomicroscope observation eggshell of the present invention outer surface figure;
Fig. 4 is eggshell ingredient of the present invention room difference figure;
Fig. 5 is each factor principal component analysis figure of eggshell of the present invention;
Fig. 6 is different cultivars egg discriminant analysis figure of the present invention;
Fig. 7 is different cultivars egg shell principal component analysis figure of the present invention;
Fig. 8 is 120 times of egg shell micro-structure diagrams of the invention;
Fig. 9 is 100 times of egg inner casing micro-structure diagrams of the invention;
Figure 10 is 1000 times of egg shell micro-structure diagrams of SEM of the present invention
Specific embodiment
Present invention will be described in further detail below with reference to the accompanying drawings, to enable those skilled in the art referring to specification text
Word can be implemented accordingly.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein do not allot one or more
The presence or addition of a other elements or combinations thereof.
Embodiment 1
Step 1: the egg identified will be needed to measure its average weight.It takes 3 pieces need to survey egg and measures preceding clear water for eggshell
Surface clean is clean, is measured after drying with thick balance, is accurate to 0.01g, average value 43.12g;According to calm and peaceful black-bone chicken egg
Average egg weight is 40.23 ± 3.6g, the average egg weight of hybridization black-bone chicken egg is 44.54 ± 4.5g, if surveyed egg size is in two groups of data
Intersection then carries out next step judgement;
Step 2: the egg identified will be needed to measure its Eggshell weight.Egg need to be surveyed by taking 3 pieces, take its eggshell, dry constant weight
After weigh, with thick balance measure, be accurate to 0.01g, 3.75g.
Step 3: the egg identified will be needed to measure its eggshell transverse diameter.Egg need to be surveyed by taking 3 pieces, with (or thousand points of vernier caliper
Ruler) it measures, middle part eggshell measures transverse diameter, is averaged, in millimeters, is accurate to 0.01mm, 3.78mm.
Step 4: obtaining discriminant equation formula Y=1.160* eggshell quality+8.922* eggshell transverse diameter-by discriminant analysis
39.404, Y value is then calm and peaceful black-bone chicken egg between -2.84 to -0.08, and Y value is then hybridization black-bone chicken egg between 0.12 to 2.46.
Between -2.84 to -0.08, preliminary judgement is calm and peaceful black-bone chicken egg for gained Y value=- 1.3288.
Step 5: being verified by stereomicroscope observation, it is more flat and smooth to meet calm and peaceful black-bone chicken egg case surface, takes a walk
There is irregular shape white dot, primitive decision is calm and peaceful black-bone chicken egg, as shown in Figure 1.
Embodiment 2
Step 1: the egg identified will be needed to measure its average weight.It takes 3 pieces need to survey egg and measures preceding clear water for eggshell
Surface clean is clean, is measured after drying with thick balance, is accurate to 0.01g, average value 41.98g;According to calm and peaceful black-bone chicken egg
Average egg weight is 40.23 ± 3.6g, the average egg weight of hybridization black-bone chicken egg is 44.54 ± 4.5g, if surveyed egg size is in two groups of data
Intersection then carries out next step judgement;
Step 2: the egg identified will be needed to measure its Eggshell weight.Egg need to be surveyed by taking 3 pieces, take its eggshell, dry constant weight
After weigh, with thick balance measure, be accurate to 0.01g, 3.54g.
Step 3: the egg identified will be needed to measure its eggshell transverse diameter.Egg need to be surveyed by taking 3 pieces, with (or thousand points of vernier caliper
Ruler) it measures, middle part eggshell measures transverse diameter, is averaged, in millimeters, is accurate to 0.01mm, 3.72mm.
Step 4: obtaining discriminant equation formula Y=1.160* eggshell quality+8.922* eggshell transverse diameter-by discriminant analysis
39.404, Y value is then calm and peaceful black-bone chicken egg between -2.84 to -0.08, and Y value is then hybridization black-bone chicken egg between 0.12 to 2.46.
Between -2.84 to -0.08, preliminary judgement is calm and peaceful black-bone chicken egg for gained Y value=- 2.11.
Step 5: being verified by stereomicroscope observation, it is more flat and smooth to meet calm and peaceful black-bone chicken egg case surface, takes a walk
There is irregular shape white dot, primitive decision is calm and peaceful black-bone chicken egg, as shown in Figure 2.
Embodiment 3
Step 1: the egg identified will be needed to measure its average weight.It takes 3 pieces need to survey egg and measures preceding clear water for eggshell
Surface clean is clean, is measured after drying with thick balance, is accurate to 0.01g, average value 43.83g;According to calm and peaceful black-bone chicken egg
Average egg weight is 40.23 ± 3.6g, the average egg weight of hybridization black-bone chicken egg is 44.54 ± 4.5g, if surveyed egg size is in two groups of data
Intersection then carries out next step judgement;
Step 2: the egg identified will be needed to measure its Eggshell weight.Egg need to be surveyed by taking 3 pieces, take its eggshell, dry constant weight
After weigh, with thick balance measure, be accurate to 0.01g, 4.04g.
Step 3: the egg identified will be needed to measure its eggshell transverse diameter.Egg need to be surveyed by taking 3 pieces, with (or thousand points of vernier caliper
Ruler) it measures, middle part eggshell measures transverse diameter, is averaged, in millimeters, is accurate to 0.01mm, 3.97mm.
Step 4: obtaining discriminant equation formula Y=1.160* eggshell quality+8.922* eggshell transverse diameter-by discriminant analysis
39.404, Y value is then calm and peaceful black-bone chicken egg between -2.84 to -0.08, and Y value is then hybridization black-bone chicken egg between 0.12 to 2.46.
Between 0.12 to 2.46, preliminary judgement is hybridization black-bone chicken egg for gained Y value=0.70.
Step 5: verified by stereomicroscope observation, meets hybridization black-bone chicken egg case surface and be distributed with and differ in size
Circular granular, difference are more obvious.Primitive decision is hybridization black-bone chicken egg, as shown in Figure 3.
Experimental verification:
1, discriminant parameter screen, as shown in figure 4, by calm and peaceful Gallus domesticlus brisson eggshell and hybridization Gallus domesticlus brisson eggshell coloration (L, a and
B value) (ingredient 1), transverse diameter and shell thickness (ingredient 2), vertical diameter and egg shape index (ingredient 3), eggshell strength and quality (ingredient 4)
Carry out comprehensive analysis discovery, the variable (being shown in Table 1) of preceding 4 principal components interpretable 82.49%, wherein principal component 1 and a and b value be just
Correlation, it is negatively correlated with L value, the 31.76% of the total variable specific gravity of Zhan;Principal component 2 and transverse diameter and shell thickness are positively correlated, the total variable of Zhan
The 19.05% of specific gravity;Principal component 3 is positively correlated with vertical diameter, is negatively correlated with egg shape index, and the 18.30% of the total variable specific gravity of Zhan;
Principal component 4 is positively correlated with eggshell strength and quality, and the 13.39% of the total variable specific gravity of Zhan, as shown in Figure 5.
Discrimination formula
Coloration (L, a and b value), transverse diameter, shell thickness, vertical diameter, egg shape index, eggshell strength and quality 9 based on eggshell
A index carries out discriminant analysis to calm and peaceful Gallus domesticlus brisson and hybridization Gallus domesticlus brisson eggshell, and research finds that the quality of eggshell and transverse diameter can be distinguished effectively
Two kinds of eggshells, as shown in Figure 6.Show that discriminant equation formula Y=1.160* eggshell quality+8.922* eggshell is horizontal by discriminant analysis
Diameter -39.404, Y value are then calm and peaceful Gallus domesticlus brisson eggshell between -2.84 to -0.08, and Y value is then hybridization crow between 0.12 to 2.46
Egg shell.Meanwhile differentiate the result shows that, the accuracy rate that the discrimination model established differentiates two kinds of egg shells is up to 83.3%.This
Discriminant analysis is verified based on the principal component where eggshell quality and eggshell transverse diameter outside, as shown in fig. 7, two kinds of egg shells can
Significantly to distinguish.Principal component analysis and discriminant analysis discovery, can effectively distinguish two kinds of eggshells based on eggshell quality and eggshell transverse diameter.
The practical application of model still needs to the application again and confirmation of mass data
3, stereomicroscope observation eggshell shell microstructure, as shown in figure 8, amplification factor be 120 × the visual field in
It can be seen that visibly different microstructure form is presented in the shell of two kinds of tested eggs: calm and peaceful black-bone chicken egg case surface is more smooth
Smooth, stroll has irregular shape white dot;The circular granular to differ in size is distributed in the eggshell outer surface of hybridization black-bone chicken egg,
Difference is more obvious
4, scanning electron microscopic observation eggshell microstructure (inner casing) 100 ×, as shown in figure 9, amplification factor be 100 × view
Yezhong can be seen, and visibly different microstructure form is presented in the inner casing of two kinds of tested eggs: calm and peaceful black-bone chicken egg inner housing surface is more
Flat and smooth, slightly cracking is evenly distributed;The eggshell inner surface for hybridizing black-bone chicken egg is that stacking is concave-convex, and difference is more obvious.
5, scanning electron microscopic observation eggshell microstructure (shell) 1000 ×, as shown in Figure 10, amplification factor be 1000 ×
The visual field in it can be seen that, visibly different microstructure form: calm and peaceful black-bone chicken egg inner housing surface is presented in the inner casing of two kinds of tested eggs
More cracking is evenly distributed, and has continuity features;The eggshell surface cracking for hybridizing black-bone chicken egg is of different sizes, does not have continuous
Property, difference is more obvious.
By stereomicroscope and scanning electron microscopic observation, calm and peaceful Gallus domesticlus brisson eggshell has in microstructure with Gallus domesticlus brisson eggshell is hybridized
Significant difference, it was demonstrated that the two difference by the feature of eggshell more can accurately identify calm and peaceful black-bone chicken egg with hybridize black-bone chicken egg.
Although the embodiments of the present invention have been disclosed as above, but it is not limited in listed fortune in specification and embodiments
With.It can be applied to various suitable the field of the invention completely.It for those skilled in the art, can be easily real
Now other modification.Therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is not limited to
Specific details and legend shown and described herein.
Claims (5)
1. the method for quick identification of calm and peaceful black-bone chicken egg and hybridization black-bone chicken egg, which comprises the following steps:
Step 1: screening: filtering out egg size in numerous eggs to be detected in the Gallus domesticlus brisson of 40.23 ± 3.6g, 44.54 ± 4.5g
Egg;
Step 2: measurement: filtering out the transverse diameter and eggshell quality of egg in measuring process one;
Step 3: identifying: differentiated by discriminant equation formula:
Y=1.160 × eggshell quality+8.922 × eggshell transverse diameter -39.494
Wherein: Y value is then calm and peaceful black-bone chicken egg between -2.84 to -0.08, and Y value is then hybridization Gallus domesticlus brisson between 0.12 to 2.46
Egg.
2. the method for quick identification of calm and peaceful black-bone chicken egg as described in claim 1 and hybridization black-bone chicken egg, which is characterized in that step 1
The black-bone chicken egg filtered out is preliminary identification, and egg size is calm and peaceful black-bone chicken egg in 40.23 ± 3.6g, and egg size is 44.54 ± 4.5g's
To hybridize black-bone chicken egg.
3. the method for quick identification of calm and peaceful black-bone chicken egg as described in claim 1 and hybridization black-bone chicken egg, which is characterized in that in step
In two, the transverse diameter is that the overall diameter of egg is accurate to 0.01mm by vernier caliper or miking.
4. the method for quick identification of calm and peaceful black-bone chicken egg as described in claim 1 and hybridization black-bone chicken egg, which is characterized in that in step
In one, when measuring egg size, need to egg be cleaned up and be dried, weighed, be accurate to using balance or high-precision platform balance
0.01g。
5. the method for quick identification of calm and peaceful black-bone chicken egg as described in claim 1 and hybridization black-bone chicken egg, which is characterized in that in step
In two, eggshell does not include matter in egg and egg film, and eggshell is cleaned up and dried, and is carried out using balance or high-precision platform balance
Weighing, is accurate to 0.01g.
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