CN109913464A - Pmel17 gene relevant to snowy peak black-bone chicken chest muscle melanin deposition and genetic marking method - Google Patents
Pmel17 gene relevant to snowy peak black-bone chicken chest muscle melanin deposition and genetic marking method Download PDFInfo
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Abstract
The invention discloses a kind of pmel17 gene and its as the genetic marker of snowy peak black-bone chicken chest muscle melanin character, by screening snowy peak black-bone chicken pmel17 gene pleiomorphism, and determine snowy peak black-bone chicken chest muscle melanin content, then pmel17 gene pleiomorphism and melanin trait associations are analyzed, it was found that in snowy peak black-bone chicken pmel17 gene as shown in sequence table, there are a G/C base mutation at 185bp, GG genotype snowy peak black-bone chicken chest muscle melanin content is significantly higher than other genotype.The present invention provides molecular labeling for the marker assisted selection of snowy peak black-bone chicken chest muscle melanin character.
Description
Technical field
The invention belongs to snowy peak black-bone chicken molecular biotechnology and breeding fields, mainly measurement and chest muscle melanin deposition
Relevant genetic marker, specifically, be measure pmel17 gene in polymorphism, i.e., with snowy peak black-bone chicken chest muscle melanin
Deposit the single nucleotide polymorphism (Singlenucleotide of related premelanosome protein 17 (pmel17) gene
Polymorphism is abbreviated as SNP) presence.
Technical background
Snowy peak black-bone chicken is the national genetic resources local varieties for originating in Hunan Province's Huaihua City, Fresh & Tender in Texture, battalion
It supports and enriches, there is health-care efficacy.Black-bone chicken medical value is related to melanin, and Compendium of Material Medica just has that " the more black person of its color, enters
Medicine is better " record, consumer is also more prone to choose the deeper Gallus domesticlus brisson of black color.And snowy peak black-bone chicken body melanin deposition individual
Between differ greatly, black degree is inhomogenous, and conventional herd breeding process is slow.With the development of biomolecule technology, allow us to from
DNA level finds major gene resistance relevant to snowy peak black-bone chicken melanin deposition or molecular labeling, and is applied in breeding
Breeding process is improved in marker assisted selection, one, obtains bigger economic benefits.
Premelanosome protein 17 (pre-melanosomal prtein17, pmel17) is that one kind synthesizes in endoplasmic reticulum
I type transmembrane transport glycoprotein, be transported in melanosome, promote melanosome structure formation, ensure the suitable of melanin
Benefit synthesis.Mankind's patients with vitiligo body leads to pmel17 malfunction, it is small to hinder melanin there are itself pmel17 antibody
The formation of body is formed to influence melanin.Research finds the one terminator codon mutation of mouse pmel17 gene and one
Mononucleotide insertion causes the mutation of additional 12 amino acid all to can produce silver color hair phenotype.Horse pmel17 gene
P.Arg618Cys mutation also can produce silver color hair phenotype；Chicken pmel17 gene contains 11 exons and 10 intrones, chicken
Pmel17 gene p.R618C mutation generates bronzing feather phenotype, and one insertion point 9bp of exon10 generates dominant white plumage
Hair phenotype.It can be seen that pmel17 gene function and melanin deposition are closely related.
The research of snowy peak black-bone chicken DNA genetic marker, genetic diversity and correlation function gene is still at an early stage, closes
In the relevant genetic marker of snowy peak black-bone chicken melanin deposition, there is not been reported.
Summary of the invention
In view of the above shortcomings of the prior art, a kind of pmel17 gene is provided and its as snowy peak black-bone chicken chest muscle melanin
The purposes of traits genetic markers；Screening SNP relevant to snowy peak black-bone chicken chest muscle melanin character simultaneously, to be applied to label
Assisted Selection or label auxiliary are penetrated into, and provide application of the above-mentioned genetic marker in snowy peak black-bone chicken marker assisted selection.
The embodiments of the present invention are implemented as follows, a kind of pmel17 gene, and the gene is and indicates snowy peak black-bone chicken chest
The relevant gene of flesh melanin deposition.
Further, the gene pmel17 sequence 185bp has base mutation G/C at one.
Further, clone includes that pmel17 gene extron 2 is as follows to exon 6 full sequence the primer:
Forward primer: 5 '-ATCGCATCCCCGTCCCTTATC-3 '
Reverse primer: 5 '-GGACCCAACCATCCCCTTGC-3 '
The another object of the embodiment of the present invention, which is to provide, a kind of uses pmel17 gene as snowy peak black-bone chicken chest muscle black
The method that element deposits related genetic marker, includes the following steps:
(1) it is covered outside the gene according to the sequence of chicken pmel17 gene in GenBank with 5.0 design primer of Primer
Son 2 is shown to exon 6 full sequence；
(2) using above-mentioned snowy peak black-bone chicken genomic DNA as template, PCR amplification, product purification, clone, acquisition such as sequence table
Nucleotide sequence shown in SEQ ID NO.1；
(3) it identifies at gene order 185bp with the presence or absence of polymorphism.
Further, the method selects snowy peak black-bone chicken as research object, screens snow using the method for molecular biology
Peak black-bone chicken chest muscle melanin trait related gene, its step are as follows:
(1) 200 progress feeding experiments of same batch health snowy peak black-bone chicken are selected；
(2) according to the primers of pmel17 gene, sample is expanded, is sequenced；
(3) SNP relevant to chest muscle melanin deposition is screened.
Snowy peak black-bone chicken related gene SNP screening
Select 200 progress feeding experiments of healthy snowy peak black-bone chicken of identical age in days.It is black to those snowy peak black-bone chicken chest muscles
Pigment content is measured, and extracts its genomic DNA respectively from high melanin content and low melanin content sample.
According to chicken pmel17 gene order in GenBank, with Primer5.0 design primer, cover the gene extron 2 to
Exon 6 full sequence, primer sequence are as follows: 5 '-ATCGCATCCCCGTCCCTTATC-3 ' (forward direction), 5 '-
GGACCCAACCATCCCCTTGC-3 ' (reversed), by Hunan, Qing Ke Bioisystech Co., Ltd is synthesized.It is right respectively with above-mentioned primer
Snowy peak black-bone chicken genomic DNA is expanded.
PCR:20 μ l reaction system: 2 × Master mix, 12 μ l, upstream and downstream primer (10 μm of ol/L)) each 0.8 μ l, DNA
1 μ l of template, ultrapure water are mended to required volume.
PCR amplification program: 34 PCR cycle parameters after 95 DEG C of 5 minutes initial denaturations are as follows: 95 DEG C 30 seconds, 62.6 DEG C 30 seconds, 72
DEG C 30 seconds, last 72 DEG C 5 minutes.
5 μ l of PCR product is taken to carry out gel electrophoresis, whether detection product clip size meets target fragment size.
30 μ l of pcr amplification product is taken, Hunan Qing Ke Bioisystech Co., Ltd is sent to be sequenced after product purification, forward direction sequencing one
A reaction, it is pmel17 gene that sequencing result, which confirms PCR product, and sequence is as shown in the SEQ ID NO:1 in table.
Fig. 1 is mutational site and sequencing peak figure.
It is above-mentioned the experimental results showed that, the melanin extracted from snowy peak black-bone chicken chest muscle is trait related because of pmel17 base
Cause, and SNP related with chest muscle melanin deposition is screened in the gene order, it is likely that here it is influence snowy peak crow bone
Thoracic muscle melanogen because.
The verifying of SNP related with snowy peak black-bone chicken chest muscle melanin in production practice
Select 44 progress feeding experiments of healthy snowy peak black-bone chicken of identical age in days and identical feeding and management condition.All confessions
Examination snowy peak black-bone chicken is butchered in 120 ages in days, measures chest muscle melanin content.
With 44 snowy peak black-bone chicken breast muscle sample genomic DNAs of extraction, and using the primer amplification in embodiment 1,
And its SNP is detected according to the method for embodiment 1.
1、PCR
PCR reaction system is as shown in the table:
Table 1PCR reaction system
PCR cycle parameter is as follows:
34 circulations: 95 DEG C, 5min；95 DEG C, 30s；62.6 DEG C, 30s, 72 DEG C, 30s；72 DEG C, 5min
2, pmel17 gene SNP site genotype frequency is analyzed
By Chi-square Test mutational site genotype frequency and gene frequency, the results are shown in Table 2, which is in
In Hardy-Weinberg balance (P > 0.05).
Table 2SNP loci gene type and gene frequency
3, the correlation analysis of pmel17 gene and snowy peak black-bone chicken chest muscle melanin character
Influence of the different SNP genotype to snowy peak black-bone chicken chest muscle melanin content is analyzed, the results are shown in Table 3 from the following table 3
It can be seen that: different SNP genotype have a significant impact snowy peak black-bone chicken chest muscle melanin；Wherein GG genotype snowy peak black-bone chicken chest muscle
The high GC type individual 57.32% of melanin content, higher by 78.20% than CC type individual, difference reaches extremely significant horizontal (P < 0.01).
GC type and CC type chest muscle melanin deposition difference is not significant.
3 different genotype chest muscle melanin content of table compares
Note: it is that difference is extremely significant (P < 0.01) that same row shoulder, which infuses different capitalizations,.
The present invention uses method of the PCR in conjunction with DNA sequencing to filter out pmel17 gene first as snowy peak black-bone chicken chest muscle
The related gene of melanin deposition then screens SNP relevant to character from snowy peak black-bone chicken pmel17 gene, and leads to
Crossing production practices and verifying determining pmel17 gene is melanin deposition related gene, and the SNP of screening is associated SNP.This
The SNP for inventing the snowy peak black-bone chicken pmel17 gene of screening can be used as the genetic marker of snowy peak black-bone chicken assisted Selection, to train
It is more to bring out melanin deposition, the black deeper snowy peak black-bone chicken of color has extremely important application value and economic benefit.
Detailed description of the invention: Fig. 1 is mutational site and sequencing peak figure
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
1 snowy peak black-bone chicken related gene SNP of embodiment screening
Select 200 progress feeding experiments of healthy snowy peak black-bone chicken of identical age in days.It is black to those snowy peak black-bone chicken chest muscles
Pigment content is measured, and extracts its genomic DNA respectively from high melanin content and low melanin content sample.
According to chicken pmel17 gene order in GenBank, with Primer5.0 design primer, cover the gene extron 2 to
Exon 6 full sequence, primer sequence are as follows: 5 '-ATCGCATCCCCGTCCCTTATC-3 ' (forward direction), 5 '-
GGACCCAACCATCCCCTTGC-3 ' (reversed), by Hunan, Qing Ke Bioisystech Co., Ltd is synthesized.It is right respectively with above-mentioned primer
Snowy peak black-bone chicken genomic DNA is expanded.
PCR:20 μ l reaction system: 2 × Master mix, 12 μ l, upstream and downstream primer (10 μm of ol/L)) each 0.8 μ l, DNA
1 μ l of template, ultrapure water are mended to required volume.
PCR amplification program: 34 PCR cycle parameters after 95 DEG C of 5 minutes initial denaturations are as follows: 95 DEG C 30 seconds, 62.6 DEG C 30 seconds, 72
DEG C 30 seconds, last 72 DEG C 5 minutes.
5 μ l of PCR product is taken to carry out gel electrophoresis, whether detection product clip size meets target fragment size.
30 μ l of pcr amplification product is taken, Hunan Qing Ke Bioisystech Co., Ltd is sent to be sequenced after product purification, forward direction sequencing one
A reaction, it is pmel17 gene that sequencing result, which confirms PCR product, and sequence is as shown in the SEQ ID NO:1 in table.Fig. 1 is prominent
Displacement point and sequencing peak figure.
It is above-mentioned the experimental results showed that, the melanin extracted from snowy peak black-bone chicken chest muscle is trait related because of pmel17 base
Cause, and SNP related with chest muscle melanin deposition is screened in the gene order, it is likely that here it is influence snowy peak crow bone
Thoracic muscle melanogen because.
The verifying of the SNP related with snowy peak black-bone chicken chest muscle melanin of embodiment 2 in production practice
Select 44 progress feeding experiments of healthy snowy peak black-bone chicken of identical age in days and identical feeding and management condition.All confessions
Examination snowy peak black-bone chicken is butchered in 120 ages in days, measures chest muscle melanin content.
With 44 snowy peak black-bone chicken breast muscle sample genomic DNAs of extraction, and using the primer amplification in embodiment 1,
And its SNP is detected according to the method for embodiment 1.
2、PCR
PCR reaction system is as shown in the table:
Table 1PCR reaction system
PCR cycle parameter is as follows:
34 circulations: 95 DEG C, 5min；95 DEG C, 30s；62.6 DEG C, 30s, 72 DEG C, 30s；72 DEG C, 5min
2, pmel17 gene SNP site genotype frequency is analyzed
By Chi-square Test mutational site genotype frequency and gene frequency, the results are shown in Table 2, which is in
In Hardy-Weinberg balance (P > 0.05).
Table 2SNP loci gene type and gene frequency
3, the correlation analysis of pmel17 gene and snowy peak black-bone chicken chest muscle melanin character
Influence of the different SNP genotype to snowy peak black-bone chicken chest muscle melanin content is analyzed, the results are shown in Table 3, from the following table 3
It can be seen that: different SNP genotype have a significant impact snowy peak black-bone chicken chest muscle melanin；Wherein GG genotype snowy peak black-bone chicken chest muscle
The high GC type individual 57.32% of melanin content, higher by 78.20% than CC type individual, difference reaches extremely significant horizontal (P < 0.01).
GC type and CC type chest muscle melanin deposition difference is not significant.
3 different genotype chest muscle melanin content of table compares
Note: it is that difference is extremely significant (P < 0.01) that same row shoulder, which infuses different capitalizations,.
<110>Hunan Hongjiang Song Yunqin industry Co., Ltd
<120>pmel17 gene relevant to snowy peak black-bone chicken chest muscle melanin deposition and genetic marking method
<130>
<160> 1
<170> PatentIn version 3.1
<210> 1
<211> 512
<212> DNA
<213>chicken (chicken)
<220>
<221> mutation
<222>185
<223>b=c or g
<400> 1
atcgcatccc cgtcccttat cccgcaggag gcggtggccg gagccgcggt ggcgttaagg 60
gctcggtgtg gggtggccgc ccggcgccgt tccggagctg ggatacagcg cggtaccgcc 120
catggcagga ggggacggcg cggcagaacg actgctggag aggtgagcgc ggggacacac 180
agacbtgggg acagacggct gcgacgccgc tccctgagcc tggctgtccc cgtgcctggc 240
aggcggcgac gtcaccttcg acatcagcaa cgatgctccc accctggtgg gcgccagggc 300
caccttctcc atcgctctgc gcttccccgg cacgcagacg gtgctgcccg acggccgcgt 360
ggtgtggagc cagaactgca ccgtcaatgg tgggcacggc tgggggcaca gctgagggca 420
cagctggagg ctcggctggg gacacagctg gggacacagc tggagacaca gctggaggtt 480
cagcggtgcc tcgctcgtgc cccctgcagg ca 512
Claims (5)
1. a kind of pmel17 gene, it is characterised in that: the gene is related to snowy peak black-bone chicken chest muscle melanin deposition is indicated
Gene, nucleotide sequence is as shown in sequence table SEQ ID NO.1.
2. pmel17 gene as described in claim 1, which is characterized in that have G/C at the pmel17 gene order 185bp
Mutation, cause polymorphism to exist.
3. the primer pair being mutated at the pmel17 gene order 185bp as described in claim 1 or claim 2 is detected,
The primer sequence is as follows:
Forward primer: 5 '-ATCGCATCCCCGTCCCTTATC-3 '
Reverse primer: 5 '-GGACCCAACCATCCCCTTGC-3 '.
4. a kind of method for using pmel17 gene to mark as snowy peak black-bone chicken chest muscle melanin deposition correlated inheritance, feature exist
In including the following steps:
(1) download chicken pmel17 gene order in GenBank, with Primer5.0 design primer, cover the gene extron 2 to
Exon 6 full sequence；
(2) using snowy peak black-bone chicken genomic DNA as template, PCR amplification, product purification, one reaction of forward direction sequencing, acquisition such as sequence
Nucleotide sequence shown in list SEQ ID NO.1；
(3) identify the gene order 185bp with the presence or absence of mutation.
5. method as claimed in claim 4, which is characterized in that the method selects snowy peak black-bone chicken as research object, adopts
Snowy peak black-bone chicken chest muscle melanin trait related gene is screened with the method for molecular biology, its step are as follows:
(1) 200 progress feeding experiments of same batch health snowy peak black-bone chicken are selected；
(2) according to the primers of pmel17 gene, sample is expanded, is sequenced；
(3) SNP relevant to chest muscle melanin deposition is screened.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116814811A (en) * | 2023-08-11 | 2023-09-29 | 江苏省家禽科学研究所 | SLC38A11 gene SNPs molecular marker primer combination and application thereof in molecular assisted breeding |
| CN117004740A (en) * | 2023-08-23 | 2023-11-07 | 江苏省家禽科学研究所 | Application of EDNRB2 gene SNP molecular marker in molecular assisted breeding of black-bone chickens |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103563851A (en) * | 2013-11-20 | 2014-02-12 | 河南农业大学 | Method for cultivating dominant-green-shin white feather broiler strain based on molecular assistant selection |
| CN103571963A (en) * | 2013-11-20 | 2014-02-12 | 河南农业大学 | Primer, kit and detection method for detecting recessive white feather locus genotype of chicken |
| CN103583470A (en) * | 2013-11-20 | 2014-02-19 | 河南农业大学 | Molecular-assisted-selection cultivation method for green-shin recessive-white-feather chicken line |
| CN103583469A (en) * | 2013-11-20 | 2014-02-19 | 河南农业大学 | Method for selecting and breeding green-shin dominant white-feather broiler chicken strain |
| CN103602745A (en) * | 2013-11-20 | 2014-02-26 | 河南农业大学 | Primers, kit and detection method for detecting gene type of dominant white feather site of chicken PMEL17 gene |
| CN104823919A (en) * | 2015-05-18 | 2015-08-12 | 贵州省畜牧兽医研究所 | Cultivation method and application of recessive white feather new strain of Xiaoxiang chicken in southeast of Guizhou province |
-
2019
- 2019-01-02 CN CN201910002048.9A patent/CN109913464B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103563851A (en) * | 2013-11-20 | 2014-02-12 | 河南农业大学 | Method for cultivating dominant-green-shin white feather broiler strain based on molecular assistant selection |
| CN103571963A (en) * | 2013-11-20 | 2014-02-12 | 河南农业大学 | Primer, kit and detection method for detecting recessive white feather locus genotype of chicken |
| CN103583470A (en) * | 2013-11-20 | 2014-02-19 | 河南农业大学 | Molecular-assisted-selection cultivation method for green-shin recessive-white-feather chicken line |
| CN103583469A (en) * | 2013-11-20 | 2014-02-19 | 河南农业大学 | Method for selecting and breeding green-shin dominant white-feather broiler chicken strain |
| CN103602745A (en) * | 2013-11-20 | 2014-02-26 | 河南农业大学 | Primers, kit and detection method for detecting gene type of dominant white feather site of chicken PMEL17 gene |
| CN104823919A (en) * | 2015-05-18 | 2015-08-12 | 贵州省畜牧兽医研究所 | Cultivation method and application of recessive white feather new strain of Xiaoxiang chicken in southeast of Guizhou province |
Non-Patent Citations (4)
| Title |
|---|
| AMER AJEEL RASHID ALHATEEMI等: "Study of chicken PMEL17 gene polymorphism using DNA sequencing method in chicken and their effects on protein function", 《LIFE SCIENCE JOURNAL》 * |
| JOANNE F. BERSON等: "Pmel17 Initiates Premelanosome Morphogenesis within Multivesicular Bodies", 《MOL BIOL CELL.》 * |
| NCBI: "Gallus gallus PMEL17 protein gene, complete cds", 《GENBANK：AY636129.1》 * |
| 彭灿阳等: "乌骨鸡黑色素沉积机理及相关候选基因", 《动物营养学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116814811A (en) * | 2023-08-11 | 2023-09-29 | 江苏省家禽科学研究所 | SLC38A11 gene SNPs molecular marker primer combination and application thereof in molecular assisted breeding |
| CN116814811B (en) * | 2023-08-11 | 2024-02-06 | 江苏省家禽科学研究所 | SLC38A11 gene SNPs molecular marker primer combination and application thereof in molecular assisted breeding |
| CN117004740A (en) * | 2023-08-23 | 2023-11-07 | 江苏省家禽科学研究所 | Application of EDNRB2 gene SNP molecular marker in molecular assisted breeding of black-bone chickens |
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