CN109913406A - Preprocess method and its application before a kind of measurement of animal sperm mobility - Google Patents
Preprocess method and its application before a kind of measurement of animal sperm mobility Download PDFInfo
- Publication number
- CN109913406A CN109913406A CN201711327639.0A CN201711327639A CN109913406A CN 109913406 A CN109913406 A CN 109913406A CN 201711327639 A CN201711327639 A CN 201711327639A CN 109913406 A CN109913406 A CN 109913406A
- Authority
- CN
- China
- Prior art keywords
- sperm
- incubation
- free medium
- serum
- incubation liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 28
- 241001465754 Metazoa Species 0.000 title claims abstract description 14
- 238000005259 measurement Methods 0.000 title claims description 7
- 238000011534 incubation Methods 0.000 claims abstract description 39
- 239000007788 liquid Substances 0.000 claims abstract description 30
- 239000012679 serum free medium Substances 0.000 claims abstract description 12
- 210000000538 tail Anatomy 0.000 claims abstract description 12
- 210000000918 epididymis Anatomy 0.000 claims abstract description 11
- 201000010063 epididymitis Diseases 0.000 claims abstract description 11
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims abstract description 10
- 229940098773 bovine serum albumin Drugs 0.000 claims abstract description 10
- 239000000725 suspension Substances 0.000 claims abstract description 7
- 239000000203 mixture Substances 0.000 claims abstract description 3
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 claims description 6
- 239000012980 RPMI-1640 medium Substances 0.000 claims description 5
- 238000004458 analytical method Methods 0.000 claims description 5
- 210000004369 blood Anatomy 0.000 claims description 3
- 239000008280 blood Substances 0.000 claims description 3
- 241000282693 Cercopithecidae Species 0.000 claims description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 2
- 238000001514 detection method Methods 0.000 abstract description 4
- 238000005457 optimization Methods 0.000 abstract description 3
- 230000037230 mobility Effects 0.000 description 19
- 230000019100 sperm motility Effects 0.000 description 9
- 239000000243 solution Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 5
- 230000033001 locomotion Effects 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 231100000027 toxicology Toxicity 0.000 description 3
- 239000003085 diluting agent Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 230000004899 motility Effects 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 210000000582 semen Anatomy 0.000 description 2
- 230000035899 viability Effects 0.000 description 2
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000036760 body temperature Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- IQFVPQOLBLOTPF-HKXUKFGYSA-L congo red Chemical compound [Na+].[Na+].C1=CC=CC2=C(N)C(/N=N/C3=CC=C(C=C3)C3=CC=C(C=C3)/N=N/C3=C(C4=CC=CC=C4C(=C3)S([O-])(=O)=O)N)=CC(S([O-])(=O)=O)=C21 IQFVPQOLBLOTPF-HKXUKFGYSA-L 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000000819 hypertonic solution Substances 0.000 description 1
- 229940021223 hypertonic solution Drugs 0.000 description 1
- 239000000815 hypotonic solution Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000033458 reproduction Effects 0.000 description 1
- 230000014639 sexual reproduction Effects 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 210000001177 vas deferen Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Preprocess method and its application before being measured the invention discloses a kind of animal sperm mobility.The preprocess method is the following steps are included: (1) takes rapidly in the sperm incubation liquid that the cauda epididymis of dead animal is placed in (37 ± 1) DEG C, pH is 6.8~7.0；The sperm incubation liquid is the serum-free medium containing 0.3~0.7% bovine serum albumin, and the percentage is quality percent by volume；(2) cauda epididymis is shredded, constant-temperature incubation 2 in Yu Suoshu sperm incubation liquid~mix after five minutes obtains sperm suspensions.The present invention so that sperm mobility detection is more quickly, accurately, and is measured reproducible by formula, pH and the time to sperm incubation of optimization sperm incubation liquid.
Description
Technical field
The present invention relates to sperm detection fields, and in particular to preprocess method before a kind of measurement of animal sperm mobility and
It is applied.
Background technique
In recent years, go deep into male reproductive toxicology research, propose with sperm morphology structure and Sperm Motility
Significant observation index is influenced Deng as thefunction of male reproduction.The locomitivity of sperm be sperm variation and function status it is comprehensive
Zoarium is existing, is the basis for completing fertilization process, all using it as the master for measuring semen quality in male reproductive toxicology research
Want one of index.In Sperm motiliy parameter, sperm motility decline will lead to fertility-rate decline.Therefore, the movement energy of sperm
Power can be used as the early stage sensitive indicator of male (hero) sexual reproduction toxicity assessment.The optimization of sperm motility measuring method is to arrenotoky poison
Pharmacological research is of great significance.
There are many factor for influencing sperm metabolism and viability, prevent adverse factor, correctly utilize favorable factor, protect sperm
There are among suitable environment, it is very crucial to extend its holding time.It influences sperm motility and an important factor for time-to-live is
Temperature, osmotic pressure and pH.37 DEG C~38 DEG C preference temperatures for being to maintain sperm proper motion, as the temperature gradually increases, essence
The mobility and metabolism of son are gradually reinforced, and energy consumption is gradually accelerated, and the time-to-live starts to shorten.Such as temperature is more than
At 50 DEG C, sperm within 5min will irreversibly devitalization power, and most cells can be by congo red staining；?
Between 20 DEG C of room temperature and 37 DEG C of body temperature, the difference of motility of sperm is fairly obvious, therefore, evaluates the product of sperm under constant temperature conditions
Matter just seems just particularly significant.Osmotic pressure be also influence sperm in vitro mobility an important factor for, sperm is only isotonic
Normal form and viability are just able to maintain in solution.Moisture is entered in spermatoblast by cell membrane in hypotonic solution, is made
Cyclic annular or semi-circular curvature occurs for sperm expansion, tail portion, and oscillating motion is dead quickly.The moisture in sperm in hypertonic solution
Saw tooth shaped crimp occurs for outside disperse, sperm shrinkage, tail portion, and movement is slowly, last dead.Therefore, in semen dilution, configuration
Isotonic spermatozoa diluent is very crucial, and used diluent is all that isotonic (under normal conditions, sperm is the grape with 6.9%
Sugar juice is isotonic), configuration dosage is accurate, and when operation prevents sperm from contacting with water.As described above, the activity of sperm in vitro
Degree is affected by various factors, and when saving and diluting sperm, is important to the pH value in view of sperm, however at present at this
It is suitable for the current not unified standard of range for the pH value that sperm in vitro is incubated in field.
Summary of the invention
The present invention be overcome in the prior art sperm mobility method for measuring time-consuming, accuracy is low and it is repeated not
Good defect, preprocess method and its application before a kind of animal sperm mobility measurement is provided.The preprocess method passes through
Optimize formula, pH and the time to sperm incubation of sperm incubation liquid, so that sperm mobility detection is more quickly, accurately,
And what is measured is reproducible.
The present invention provides the preprocess method before a kind of measurement of animal sperm mobility comprising following steps:
(1) it takes rapidly in the sperm incubation liquid that the cauda epididymis of dead animal is placed in (37 ± 1) DEG C, pH is 6.8~7.0；
The sperm incubation liquid is the serum-free medium containing 0.3~0.7% bovine serum albumin, and the percentage is quality volume basis
Than；
(2) cauda epididymis is shredded, constant-temperature incubation 2 in Yu Suoshu sperm incubation liquid~mix after five minutes obtains sperm
Suspension.If less than 2 minutes constant-temperature incubation time sperm cannot abundant separate out, if more than 5 minutes sperm motilities
Decline.
Wherein, serum-free medium described in step (1) can be the serum-free medium of this field routine, preferably
RPMI1640 or DMEM.According to the present invention, RPMI1640 and DMEM contains cultivates ingredient substantially required for cell culture,
It is equally applicable for the incubation of sperm in the present invention.
Sperm incubation liquid described in step (1) is preferably the serum-free medium for containing 0.5% bovine serum albumin, described
Percentage is quality percent by volume.When the quality percent by volume of contained bovine serum albumin in the sperm incubation liquid is lower than
0.3% or be higher than 0.7% when, bovine serum albumin cannot play the role of physiology and mechanical protection and carrier function.
The time of constant-temperature incubation described in step (2) is no more than 3 minutes.
In the preprocess method, the animal can be the small-sized mammalian of this field routine, preferably monkey, rabbit
Son, mouse or rat.
The present invention also provides a kind of measuring method of the sperm mobility of non-diagnostic purpose, the measuring method includes above-mentioned
Preprocess method.Preferably, detecting the sperm suspension using compater assisted sperm analysis after the preprocess method
Liquid.
The present invention also provides a kind of sperm incubation liquid, which is characterized in that it is 0.3~0.7% bovine serum albumin without blood
Clear culture solution, the percentage are quality percent by volume.Preferably, the pH of the sperm incubation liquid is 6.8~7.0.Work as pH
When being worth not in the range, motility of sperm, which can be reduced quickly, even there is death.When the pH of the sperm incubation liquid is higher than
When 7.0, sperm motility is rapid, the time-to-live is short.When pH is lower than 6.8, sperm motility is slow, the time-to-live is long.
The present invention also provides a kind of application of above-mentioned sperm incubation liquid before animal sperm mobility measures in terms of pretreatment.
On the basis of common knowledge of the art, above-mentioned each optimum condition, can any combination to get each preferable reality of the present invention
Example.
The reagents and materials used in the present invention are commercially available.
The positive effect of the present invention is that:
The present invention passes through formula, pH and the time to sperm incubation of optimization sperm incubation liquid, so that sperm mobility
Detection more quickly, accurately, and measures reproducible.
Specific embodiment
The present invention is further illustrated below by the mode of embodiment, but does not therefore limit the present invention to the reality
It applies among a range.In the following examples, the experimental methods for specific conditions are not specified, according to conventional methods and conditions, or according to quotient
The selection of product specification.
Embodiment 1
One, preparation
1, the preparation of sperm incubation liquid
It weighs 0.5g bovine serum albumin(BSA) (BSA is purchased from Sinopharm Chemical Reagent Co., Ltd.) and is dissolved in 100mL without blood
Clear DMEM (being purchased from gibco company), adjusts pH to 7.0.
2, instrument temperature is adjusted
The temperature of water-bath, insulating box is adjusted to (37 ± 1) DEG C.
3, surgical instrument and utensil constant temperature
The eye scissors for shredding cauda epididymidis are placed in the test tube equipped with sperm incubation liquid, (37 ± 1) DEG C water-bath is placed in, it will
Pipettor gun is first-class to be placed in insulating box.
4,5mL centrifuge tube is taken, the above-mentioned sperm incubation liquid being prepared of 3.9mL is added in it.
Two, the pretreatment before sperm mobility measurement
1, healthy rat is implemented and is euthanized；
2, its left testes and cauda epididymis are exposed rapidly；
3, cauda epididymis is cut in cauda epididymis and vas deferens bifurcation, weigh and recorded；
4, the cauda epididymis removed is put into the sperm incubation liquid prepared in (37 ± 1) DEG C above-mentioned first part；
5, cauda epididymis is shredded with the eye scissors that sperm incubation liquid is kept the temperature, constant temperature 2~3 minutes, swims out of sperm；Then
It mixes, obtains sperm suspensions.
6, rat spermatozoa suspension 0.1mL is drawn with the pipette tips of preheating to be added equipped with the sperm prepared in above-mentioned first part
The 5mL test tube of Incubating Solution is used for sperm mobility inspection.
Embodiment 2
In addition to constant temperature time is 4~5 minutes in second part step 5, remaining same embodiment 1.
Comparative example
In addition to the pH > 7 of sperm incubation liquid in first part's step 1, remaining same embodiment 1.
Application Example sperm mobility inspection
Sperm mobility inspection is carried out by pretreated sperm to what above-described embodiment 1,2 and comparative example obtained, is used
Hamilton Thorne computer assisted sperm analysis instrument is detected with RAT Head Toxicology software: entering setup mesh
Record, successively sets analysis, optical and stage parameter is sequentially input into information screen
SETUP INFORMATION, STUDY INFORMATION (use analysis the same day date as Study Number) and
SAMPLE INFORMATION.It completes to detect into the interface Acquire.The testing result corresponding with comparative example of embodiment 1,2
Respectively table 1,2 and 3.
The result (Incubating Solution pH=7) that table 1 detects after being incubated for 2~3 minutes
Table 2 is incubated for the 4~result (Incubating Solution pH=7) that detects after five minutes
The result (Incubating Solution pH > 7) that table 3 detects after being incubated for 2~3 minutes
Tables 1 and 2 data are examined using Mana-Whitney U, the sperm motility after being incubated for 4~5 minutes and 2~3 minutes
Degree can satisfy the demand for carrying out subsequent experimental or processing to it in the art；And the sperm motility after being incubated for 2~3 minutes
Degree is apparently higher than the sperm mobility (p < 0.05) of incubation 4~after five minutes.
Table 1 and 3 data of table are examined using Mana-Whitney U, with Incubating Solution pH=7 testing result ratio, utilize pH > 7
The sperm mobility that is incubated for of Incubating Solution be substantially less than the sperm mobility after two groups of sperm mobilities are incubated for 2~3 minutes (p <
0.05)。
Claims (10)
1. the preprocess method before a kind of measurement of animal sperm mobility, which is characterized in that itself the following steps are included:
(1) it takes rapidly in the sperm incubation liquid that the cauda epididymis of dead animal is placed in (37 ± 1) DEG C, pH is 6.8~7.0；It is described
Sperm incubation liquid is the serum-free medium containing 0.3~0.7% bovine serum albumin, and the percentage is quality percent by volume；
(2) cauda epididymis is shredded, constant-temperature incubation 2 in Yu Suoshu sperm incubation liquid~mix after five minutes obtains sperm suspension
Liquid.
2. preprocess method as described in claim 1, which is characterized in that serum-free medium described in step (1) is
RPMI1640 or DMEM.
3. preprocess method as described in claim 1, which is characterized in that Incubating Solution described in step (1) is containing 0.5% ox blood
Albuminised serum-free medium, the percentage are quality percent by volume.
4. preprocess method as described in claim 1, which is characterized in that the time of constant-temperature incubation described in step (2) does not surpass
Spend 3 minutes.
5. such as the described in any item preprocess methods of Claims 1 to 4, which is characterized in that the animal is monkey, rabbit, small
Mouse or rat.
6. a kind of measuring method of the animal sperm mobility of non-diagnostic purpose, which is characterized in that the measuring method includes such as
The described in any item preprocess methods of Claims 1 to 5.
7. measuring method as claimed in claim 6, which is characterized in that the described method includes: after the preprocess method, it will
The sperm suspensions detect wherein sperm mobility using compater assisted sperm analysis.
8. a kind of sperm incubation liquid, which is characterized in that it is the serum-free medium containing 0.3~0.7% bovine serum albumin, described
Percentage is quality percent by volume；Preferably, the serum-free medium is RPMI1640 or DMEM.
9. sperm incubation liquid as claimed in claim 8, which is characterized in that the pH of the sperm incubation liquid is 6.8~7.0.
10. a kind of serum-free medium containing 0.3~0.7% bovine serum albumin is preparing the application in sperm incubation liquid, described
Percentage is quality percent by volume；Preferably, the serum-free medium is RPMI1640 or DMEM.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711327639.0A CN109913406A (en) | 2017-12-13 | 2017-12-13 | Preprocess method and its application before a kind of measurement of animal sperm mobility |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711327639.0A CN109913406A (en) | 2017-12-13 | 2017-12-13 | Preprocess method and its application before a kind of measurement of animal sperm mobility |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109913406A true CN109913406A (en) | 2019-06-21 |
Family
ID=66958560
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711327639.0A Pending CN109913406A (en) | 2017-12-13 | 2017-12-13 | Preprocess method and its application before a kind of measurement of animal sperm mobility |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109913406A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114459857A (en) * | 2022-01-29 | 2022-05-10 | 上海益诺思生物技术股份有限公司 | Pretreatment method for maintaining animal sperm motility and determining animal sperm apoptosis |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103006930A (en) * | 2012-11-30 | 2013-04-03 | 重庆市中药研究院 | Application of ginseng and rhizoma curculiginis combined medicinal herbs in preparation of medicament for treating male infertility |
| CN104435353A (en) * | 2014-11-14 | 2015-03-25 | 马静 | Combined medicine for treating male infertility and application thereof |
| CN104498584A (en) * | 2014-12-31 | 2015-04-08 | 云南农业大学 | Fluorescent staining method of evaluating sperm motility of tree shrew |
| CN105154516A (en) * | 2015-10-15 | 2015-12-16 | 中国农业大学 | Quality detection method of experimental primate animal sperms |
-
2017
- 2017-12-13 CN CN201711327639.0A patent/CN109913406A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103006930A (en) * | 2012-11-30 | 2013-04-03 | 重庆市中药研究院 | Application of ginseng and rhizoma curculiginis combined medicinal herbs in preparation of medicament for treating male infertility |
| CN104435353A (en) * | 2014-11-14 | 2015-03-25 | 马静 | Combined medicine for treating male infertility and application thereof |
| CN104498584A (en) * | 2014-12-31 | 2015-04-08 | 云南农业大学 | Fluorescent staining method of evaluating sperm motility of tree shrew |
| CN105154516A (en) * | 2015-10-15 | 2015-12-16 | 中国农业大学 | Quality detection method of experimental primate animal sperms |
Non-Patent Citations (5)
| Title |
|---|
| DINESH TIWARI等: "Mutagenic effect of Bisphenol A on adult rat male germ cells and their fertility", vol. 40, pages 60 - 68, XP028684154, DOI: 10.1016/j.reprotox.2013.05.013 * |
| 于利: "大鼠睾丸的Sertoli细胞的分离纯化及体外培养", no. 03, pages 204 - 205 * |
| 卢锋;乔玲;马远方;: "番茄红素对实验小鼠免疫功能的影响", no. 02, pages 151 - 155 * |
| 张春燕;刘丽均;徐平;芮荣;: "大鼠体外受精技术", no. 08, pages 107 - 110 * |
| 王立蕊;甄林青;杨强震;徐芳;张宇静;谢硕;冯健;李新红;: "培养液pH值对小鼠精子体外培养过程中活力及蛋白修饰的影响", no. 03, pages 10 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114459857A (en) * | 2022-01-29 | 2022-05-10 | 上海益诺思生物技术股份有限公司 | Pretreatment method for maintaining animal sperm motility and determining animal sperm apoptosis |
| CN114459857B (en) * | 2022-01-29 | 2023-09-05 | 上海益诺思生物技术股份有限公司 | Pretreatment method for maintaining animal sperm motility and animal sperm apoptosis determination |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Tanga et al. | Semen evaluation: Methodological advancements in sperm quality-specific fertility assessment—A review | |
| Ramu et al. | The hypo-osmotic swelling test for evaluation of sperm membrane integrity | |
| Perfetto et al. | Amine‐reactive dyes for dead cell discrimination in fixed samples | |
| Pinkel et al. | Flow cytometric determination of the proportions of X-and Y-chromosome-bearing sperm in samples of purportedly separated bull sperm | |
| Sigman et al. | Semen analysis and sperm function assays: what do they mean? | |
| Lopes et al. | Respiration rates of individual bovine in vitro-produced embryos measured with a novel, non-invasive and highly sensitive microsensor system | |
| Leelatian et al. | Characterizing phenotypes and signaling networks of single human cells by mass cytometry | |
| Esteves et al. | Evaluation of acrosomal status and sperm viability in fresh and cryopreserved specimens by the use of fluorescent peanut agglutinin lectin in conjunction with hypo-osmotic swelling test | |
| JP7124062B2 (en) | Method for detecting the presence of an analyte in a urine sample | |
| Egeberg Palme et al. | Viable acrosome-intact human spermatozoa in the ejaculate as a marker of semen quality and fertility status | |
| van der Horst et al. | Not just the marriage of Figaro: but the marriage of WHO/ESHRE semen analysis criteria with sperm functionality | |
| Dolník et al. | Flow cytometry in assessment of sperm integrity and functionality–a review | |
| Giudice et al. | Optimization and standardization of fluorescent cell barcoding for multiplexed flow cytometric phenotyping | |
| CN101126758A (en) | Flow cytometry synchronous detection method for multiple protein expression of tumor cell | |
| Lombó et al. | Sperm metabolomics through nuclear magnetic resonance spectroscopy | |
| Colton et al. | Characterization of islet preparations | |
| CN110608991A (en) | Cell cycle detection kit based on mass flow detection technology and detection method | |
| CN109913406A (en) | Preprocess method and its application before a kind of measurement of animal sperm mobility | |
| Zarchi et al. | The effects of in vitro incubation of asthenoteratozoospermic semen after density gradient centrifugation at room temperature and 37 C on sperm parameters, chromatin quality and DNA fragmentation in a short time period | |
| CN112985966A (en) | Diluent for analyzing urine visible components and preparation method thereof | |
| Dias | Measurement of reactive oxygen species in semen samples using chemiluminescence | |
| CN108152189B (en) | Quantitative detection method for sperm surface clouding protein 1 | |
| CN114875108A (en) | In vitro embryo development potential prediction and evaluation technology based on glucose and glutamine level test | |
| Zeng et al. | Multiplexed detection and the establishment of a novel high-throughput method for human germ cell quality screening based on aggregation-induced emission | |
| Brombacher et al. | Characterization of Dendritic Cell Metabolism by Flow Cytometry |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: 199 GuoShouJing Road, Pudong New Area pilot Free Trade Zone, Shanghai, 201203 Applicant after: Shanghai Yinuosi biotechnology Limited by Share Ltd. Address before: 201203 No. 199, Guo Shoujing Road, China (Shanghai) Free Trade Pilot Zone, Pudong New Area, Shanghai Applicant before: Shanghai Yinuosi biotechnology Limited by Share Ltd. |