CN109893520A - The new application of spirulina gamma-Linolenic acid - Google Patents
The new application of spirulina gamma-Linolenic acid Download PDFInfo
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- CN109893520A CN109893520A CN201910215558.4A CN201910215558A CN109893520A CN 109893520 A CN109893520 A CN 109893520A CN 201910215558 A CN201910215558 A CN 201910215558A CN 109893520 A CN109893520 A CN 109893520A
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Abstract
The present invention relates to a kind of spirulina gamma-Linolenic acid prepare wound reparation, the purposes in anti-inflammatory or antiallergy product.The product is zebra fish wound preparation for repairing or the product behaviour wound reparation, anti-inflammatory or antianaphylactic product.The present invention utilizes living body (zebra fish juvenile fish) for the first time the experiment proves that spirulina gamma-Linolenic acid is in doses or concentration range, with following bioactivity: wound reparation accelerates regeneration, Scavenger of ROS, anti-inflammatory and antiallergy, and also these activity have certain dosage effective range.Application of the spirulina gamma-Linolenic acid in the fields such as drug, food, cosmetics and feed is claimed in above-mentioned activity based on spirulina gamma-Linolenic acid, the present invention.
Description
Technical field
Invention belongs to medicine, food or cosmetic technical field, and in particular to spirulina gamma-Linolenic acid is in preparation for creating
New application in mouth reparation, anti-inflammatory or antiallergy product.
Background technique
Spirulina (Spirulina) it is under the jurisdiction of Cyanophyta, it is one kind filamentous as made of the aggregation of unicellular or many cells
Prokaryotes, also referred to as " arthrospira (Arthrospira) ".The current spirulina manually cultivated on a large scale both at home and abroad, mainly has
Blunt top spirulina (Arthrospira platensis ) and spirulina maxim (Arthrospira maxima) etc. types.Spiral shell
Algae is revolved rich in good protein, polysaccharide, carotenoid, gamma-Linolenic acid, several mineral materials and vitamin etc.1.Gamma-Linolenic acid
It is gradually concerned by people due to multiple biological activities, the gamma-Linolenic acid in spirulina forms glycolipid in conjunction with lactose
(main to include single galactolipin diacetyl glycerolipid and double galactolipin diacetyl glycerolipid), has both the function of gamma-Linolenic acid and glycolipid
Can, it makes it have and is more widely applied range and value1。
Gamma-Linolenic acid is GAMMA-Linolenic acid, is the serial polyunsaturated fatty acid of ω -6.γ-flax
The important physiological activity of acid is that he is the precursor of human body synthesis of prostaglandins2.In addition to this, gamma-Linolenic acid closes rheumatic
Section is scorching3,4, eczema5, bowel syndrome6,7And neuralgia8Etc. diseases all there is relaxation effect.
Gamma-Linolenic acid currently on the market is mainly derived from the seed of higher plant, and since raw material supply is limited, utilization is micro-
The method of biofermentation becomes a kind of alternative route for preparing gamma-Linolenic acid.But pass through Production by Microorganism Fermentation γ-at present
Linolenic yield is still relatively low, is difficult to realize industrialization.Also, there are a large amount of undesirable rouge in fungal oil extract
Fat acid obtains gamma-Linolenic acid and needs laborious and expensive purification step.
Glycolipid type gamma-Linolenic acid accounts for about the 2% of spirulina dry weight in spirulina, is one of ideal source of gamma-Linolenic acid,
But the activity research of the gamma-Linolenic acid in spirulina source is very limited, and there has been no the reports of animal intracorporeal active experiment.
The results of in vitro studies such as Wang Fei show that spirulina gamma-Linolenic acid has antioxidation activity in vitro9.Wang F's etc. research shows that from
The gamma-Linolenic acid extracted in spirulina has the function of inducing hepatocellular carcinoma H22 apoptosis and antithrombotic10。Esquivel-
Hernandez DA's etc. research shows that spirulina gamma-Linolenic acid has the function of antibacterial11.Therefore, carry out spirulina γ-flax
Sour intracorporeal active experiment determines its bioactivity and function, has for the commercial applications of spirulina gamma-Linolenic acid important
Directive function.
In addition, gamma-Linolenic acid reported at present is mainly triglycerides form, i.e., gamma-Linolenic acid is in the form of side chain
It is incorporated on glycerol molecule.And the gamma-Linolenic acid in spirulina is present in the membrane structure of cell in the form of glycolipid.Although
Have more activity research for the gamma-Linolenic acid of triglycerides form, but for the gamma-Linolenic acid of glycolipid form activity
Research be but still rarely reported.Spirulina glycolipid type gamma-Linolenic acid activity and functional study extremely lack, especially living in vivo
Property experiment shortage, limit the application of spirulina gamma-Linolenic acid.
Therefore, for further study to the bioactivity of spirulina gamma-Linolenic acid and discussion, to spirulina gamma-Linolenic acid
Development and application direction and field have very important directive significance.
Relevant references:
1.Cohen Z, Reungjitchachawali M, Siangdung W, et al. Production and
partial purification of γ-linolenic acid and some pigments from Spirulina
platensis[J]. Journal of Applied Phycology, 1993, 5: 109-115.
2.Kim, D.-H., Yoo, T.-H.; Lee, S.H.; Kang, H.Y.; Nam, B.Y.; Kwak, S.J.;
Kim, J.-K.; Park, J.T.; Han, S.H.; Kang, S.-W. Gamma Linolenic Acid Exerts
Anti-Inflammatory and Anti-Fibrotic Effects in Diabetic Nephropathy. Yonsei Med. J. 2012, 53, 1165–1175.
3.Zurier, R.B.; Rossetti, R.G.; Jacobson, E.W.; DeMarco, D.M.; Liu, N.Y.;
Temming, J.E.; White, B.M.; Laposata, M. Gamma linolenic acid treatment of
rheumatoid arthritis. A randomized, placebo-controlled trial. Arthritis Rheum. 1996, 39, 1808–1817.
4.Kast, R.E. Borage oil reduction of rheumatoid arthritis activity may be
mediated by increased cAMP that suppresses tumor necrosis factor-alpha. Int. Immunopharmacol. 2001, 1, 2197–2199.
5.Worm, M.; Henz, B.M. Novel unconventional therapeutic approaches to
atopic eczema. Dermatology 2000, 201, 191–195.
6.Ponnala, S.; Rao, K.P.; Chaudhury, J.R.; Ahmed, J.; Rama Rao, B.;
Kanjilal, S.; Hasan, Q.; Das, U.N. Effect of polyunsaturated fatty acids on
diphenyl hydantoin-induced genetic damage in vitro and in vivo.Prostaglandins Leukot. Essent. Fatty Acids 2009, 80, 43–50
7.Bugnariu, S. Beneficial effect of gamma-linolenic acid-enriched diet
therapeutic properties of Oenothera biennis oil (evening primrose oil).Farmacia1996, 44, 3–4.
8.Wanasundara, U.N.; Wanasundara, J.P.D.; Akoh, C.C. γ-Linolenic acid:
purification and functionality. In Handbook of Functional Lipids 2006; pp.
47–75.
9. the extraction of Wang Fei, She Xingxing, Sun Bingjie, Du Jiachen, Zhang Jiawei, Ren Difeng, Lu Jun spirulina gamma-Linolenic acid optimizes
And the research food industry science and technology of antioxidation activity in vitro, 2014,35 (19): 68-72.
10.Wang, F.; Miao, M.; Chen, B.; Wang, R.; Sun, B.; Ren, D.; Lu, J.
Antineoplastic activity of γ-linolenic acid extract from Spirulina platensis
on HepG2 cells and its inhibition effect on platelet aggregation. Food Agric.
Immunol. 2014, 26, 97–108.
11.Esquivel-Hernandez, D.A.; Rodriguez-Rodriguez, J.; Cuellar-Bermudez,
S.P.; Garcia-Perez, J.S.; Mancera-Andrade, E.I.; Nunez-Echevarria, J.E.;
Ontiveros-Valencia, A.; Rostro-Alanis, M.; Garcia-Garcia, R.M.; Torres, J.A.
et al. Effect of Supercritical Carbon Dioxide Extraction Parameters on the
Biological Activities and Metabolites Present in Extracts from Arthrospira
platensis. Mar. Drugs 2017, 15, 174。
Summary of the invention
(1) technical problems to be solved
In view of the above problems, inventor has made intensive studies the activity of the gamma-Linolenic acid in spirulina source, it has unexpectedly been found that
Spirulina gamma-Linolenic acid in wound reparation, accelerate regeneration, Scavenger of ROS, the biology in terms of anti-inflammatory and antiallergy is living
Property and application.
(2) technical solution
In order to achieve the above object, the main technical schemes that the present invention uses include:
Spirulina gamma-Linolenic acid prepare wound reparation, the purposes in anti-inflammatory or antiallergy product.
Further, application of the spirulina gamma-Linolenic acid in preparation zebra fish wound reparation preparation.
Further, purposes of the spirulina gamma-Linolenic acid in preparation people's wound reparation, anti-inflammatory or antiallergy product.
Further, the product is drug, cosmetics, food or feed.
Further, the product is externally applied product, such as lotion, soaking agent, spray, paste or creme.
Preferably, the preparation is soaking agent, and in the soaking agent, and the concentration of spirulina gamma-Linolenic acid is 50mg/
L~100mg/L；More preferably 100mg/L.
Preferably, the product is food or feed.
Preferably, the food or feed are liquid product；In the liquid product, spirulina gamma-Linolenic acid it is dense
Degree is 100mg/L.
Experiment shows: spirulina gamma-Linolenic acid is within the scope of the solution concentration of 50mg/L ~ 100mg/L, with wound or wound
It directly contacts, has the function of accelerating wound tissue repair；
Zebra fish juvenile fish is impregnated with the solution of the spirulina gamma-Linolenic acid containing 100mg/L, fish body can be improved and removed after being wound
The ability of active oxygen near its wound；
Zebra fish juvenile fish is impregnated with the solution of the spirulina gamma-Linolenic acid containing 100mg/L, it is anti-after being wound that fish body can be improved
Scorching and antiallergy ability.
In conclusion spirulina gamma-Linolenic acid within the scope of the solution concentration of 50mg/L ~ 100mg/L, has following work
Property: accelerate wound regeneration, remove wound nearby active oxygen, there is anti-inflammatory activity in early stage of being wound, have in the later period of being wound
Antiallergic activity.
(3) beneficial effect
The beneficial effects of the present invention are:
The present invention demonstrates spirulina gamma-Linolenic acid in doses or concentration range using experiment made on the living for the first time, under having
Column bioactivity: wound reparation accelerates regeneration, Scavenger of ROS, anti-inflammatory and antiallergy.The present invention is to spirulina γ-Asia
The development and application of numb acid gives direction.
The above-mentioned function of spirulina gamma-Linolenic acid makes it have important open in the fields such as food, cosmetics and feed
Hair and application value.
The present invention utilize zebra fish for the first time the experiment proves that spirulina gamma-Linolenic acid above-mentioned bioactivity, and due to big
About 82% study of human disease-related gene position all has homologous gene in zebra fish, therefore zebra fish has become drug screening, drug
The valid model of therapeutic evaluation and the parsing of drug effect molecular mechanism.Therefore, the present invention selects zebra fishtail fin injury repair mould
Type has carried out experimental verification to the activity of spirulina glycolipid type gamma-Linolenic acid, and the conclusion of experimental verification is also applied for mankind's wound
Related products, such as cosmetics such as mouth reparation, regeneration, anti-inflammatory antiallergy.
Detailed description of the invention
The A of Fig. 1 is after zebra fish juvenile fish tail fin is removed, to raise the spiral in 0,50,100,250,500 mg/L respectively
After raising 24,48,72,96,120 hours in algae gamma-Linolenic acid solution, tail fin wound and regeneration situation are observed, and use body
The photo of stereomicroscope (AF205, Leica) shooting；
The B of Fig. 1 is after zebra fish juvenile fish tail fin is removed, to raise the spirulina in 0,50,100,250,500 mg/L respectively
In gamma-Linolenic acid solution, the zebra fish juvenile fish tail fin length (μm) of measurement with feeding time change curve.
The A of Fig. 2 be zebra fish juvenile fish respectively 0,100, raising 24 is small in the spirulina gamma-Linolenic acid solution of 250mg/L
Shi Hou cuts off its part tail fin, the photo of the fluorescence intensity of active oxygen near observation zebra fish juvenile fish wound；
The B of Fig. 2 be zebra fish juvenile fish respectively 0,100, raise 24 hours in the spirulina gamma-Linolenic acid solution of 250mg/L after,
Its part tail fin is cut off, fluorescence intensity-zebra fish juvenile fish tail fin of active oxygen is long near the zebra fish juvenile fish wound observed
Spend (μm) graph of relation.
After the A of Fig. 3 is raised 24 hours in the spirulina gamma-Linolenic acid solution of 0,100mg/L respectively for zebra fish juvenile fish,
Its part tail fin is cut off, observation 20 minutes after a resection, neutrophil leucocyte quantity at 2,4,8 hours wounds, and uses Leica
The photo of 6000 microscope photographing of DMI.
After the B of Fig. 3 is raised 24 hours in the spirulina gamma-Linolenic acid solution of 0,100mg/L respectively for zebra fish juvenile fish,
Its part tail fin is cut off, the quantity of the neutrophil leucocyte at 0.2,2,4,8 hour wound after a resection is counted.
Specific embodiment
In order to preferably explain the present invention, in order to understand, with reference to the accompanying drawing, by specific embodiment, to this hair
It is bright to be described in detail.
Key point of the invention is to demonstrate spirulina gamma-Linolenic acid in doses or concentration using experiment made on the living for the first time
In range, with following bioactivity: wound reparation, acceleration regeneration, Scavenger of ROS, anti-inflammatory and antiallergy, and this
A little activity have certain dosage effective range.
Spirulina gamma-Linolenic acid is claimed in drug, food in above-mentioned activity based on spirulina gamma-Linolenic acid, the present invention
Application in the fields such as product, cosmetics and feed.
It is that spirulina gamma-Linolenic acid is applied to wild type AB plants of zebra fish below to prove related conclusions of the present invention
Experimentation and corresponding experimental result.
One, experimental material:
(1) spirulina glycolipid type gamma-Linolenic acid: from spirulina powder (graning century-old bioengineering Co., Ltd purchased from Jiangsu),
It extracts and purifies according to method disclosed in embodiment 1 in the patent application CN107874253A announced.
(2) wild type AB plants of zebra fish and Tg (mpx: EGFP) transgenic zebrafish are by Chinese Academy of Sciences aquatile
Zebra fish resource center, country, research institute (http://zfish.cn) provides, safeguards and raises.
Two, experimental program
(1) purpose of this experiment is: after the excision of zebra fish juvenile fish tail fin part, being immersed in spirulina γ-Asia of various concentration
In numb acid solution, the reparative regeneration situation of wound is observed.Experiment specific steps are as follows:
(1) 54 hours AB plants of zebra fish juvenile fish of wild type carry out this experiment after selective fertilization.
(2) at anatomical lens (Olympus), zebra fish juvenile fish notochord is cut off along the direction vertical with notochord with scalpel
The part tail fin of tail end.
(3) it prepares the spirulina gamma-Linolenic acid solution of various concentration: spirulina gamma-Linolenic acid is dissolved in dimethyl sulfoxide,
The gamma-Linolenic acid liquid storage for preparing 50mg/ml, with 0.3 × Danieau buffer, (Danieau buffer contains: 17.4 mM
NaCl, 0.21 mM KCl, 0.12 mM MgSO4, 0.18 mM Ca(NO3)2, 1.5 mM HEPES, pH 7.6), then
Spirulina gamma-Linolenic acid liquid storage is diluted to the working concentration of needs.
(4) the zebra fish juvenile fish that part tail fin is cut off individually is raised in 6 orifice plates, the hole 2ml/ is previously added in hole
The spirulina gamma-Linolenic acid solution of various concentration (50,100,250,500 mg/L).Each concentration makees the sample of 12 fishes,
Blank control group is done with 0.1% dimethyl sulfoxide dilution.
(5) after zebra fish juvenile fish part tail fin is cut off 24,48,72,96,120 hours, tail fin wound and again is observed
Raw situation, and take pictures.Before taking pictures, zebra fish juvenile fish first uses 0.015% tricaine (being diluted with 0.3 × Danieau buffer)
Anesthesia, then be placed on 2% methylcellulose liner plate, it is taken pictures with stereomicroscope (AF205, Leica).
(6) zebra fish juvenile fish has been clapped to be placed in 0.3 × Danieau buffer after photograph and recover, and is then put back into fresh
Continue to cultivate in the gamma-Linolenic acid solution of configuration, until experiment terminates.
Experimental result impregnates zebra wound as shown in Figure 1, adding 50mg/L and 100mg/L spirulina gamma-Linolenic acid in hole
Mouthful, zebra fish juvenile fish tail fin wound reparation can be substantially speeded up, and use high concentration spirulina γ-Asia of 250mg/L and 500mg/L
Numb acid soak then makes zebra fish juvenile fish tail fin wound reparation slow down.The results showed that the gamma-Linolenic acid of low concentration can accelerate
Wound reparation, and the gamma-Linolenic acid of high concentration can then inhibit wound reparation.
(2) purpose of this experiment is: it is small that zebra fish juvenile fish is supported in various concentration spirulina gamma-Linolenic acid solution 24
Shi Houzai cuts off the part tail fin of zebra fish juvenile fish, observes removing of the zebra fish juvenile fish of separate sources to active oxygen at its wound
Ability.Experiment specific steps are as follows:
(1) 48 hours AB plants of zebra fish juvenile fish of wild type carry out this experiment after selective fertilization.
(2) zebra fish juvenile fish is individually supported in 6 orifice plates, be previously added in hole the hole 2ml/ various concentration (50,
100,250 mg/L) spirulina gamma-Linolenic acid solution and 0.1% dimethyl sulfoxide dilution (blank control group), each concentration
Do 10 fishes.
(3) after zebra fish juvenile fish is temporarily supported 24 hours in above-mentioned (2) step, first with 0.015% tricaine (with 0.3 ×
The dilution of Danieau buffer) anesthesia, then with scalpel along the direction excision zebra fish juvenile fish notochord tail end vertical with notochord
Part tail fin.
(4) juvenile fish that part tail fin is removed is placed in 1ml ROS detection kit (ENZO, Cat. No:ENZ-
In working solution in 51042-K500), it is protected from light holding 10 minutes.
(5) it after being washed zebra fish juvenile fish three times with HBSS, is placed on 2% methylcellulose liner plate, with Leica DMI
6000 microscopes are taken pictures.
(6) fluorescence intensity quantifies: 8 bit images being imported Fiji software and are converted to 32, are selected with the rectangle tool
" ROI " manager is simultaneously applied in wound area, utilizes the fluorescence intensity of " Plot Profile " functional examination selected areas.
As a result as shown in Fig. 2, being soaked for a period of time later zebra fish juvenile fish in 100mg/L spirulina gamma-Linolenic acid,
The ability that fish body removes active oxygen near wound can be improved, and the spirulina gamma-Linolenic acid of 250mg/L is then without this effect.
(3) purpose of this experiment is: it is small that zebra fish juvenile fish is supported in various concentration spirulina gamma-Linolenic acid solution 24
Shi Houzai cuts off the part tail fin of zebra fish juvenile fish, observes neutrophil leucocyte quantity at its wound of the zebra fish juvenile fish of separate sources
And inflammatory conditions.
Experiment specific steps are as follows:
(1) 48 hours Tg (mpx: EGFP) transgenic zebrafish juvenile fish carry out this experiment after selective fertilization.
(2) zebra fish juvenile fish is individually supported in 6 orifice plates, γ-Asia of 100 mg/L of the hole 2ml/ is previously added in hole
Numb acid solution and 0.1% dimethyl sulfoxide dilution (blank control group), every group is done 10 fishes.
(3) after zebra fish juvenile fish is temporarily supported 24 hours in above-mentioned a step, first with 0.015% tricaine (with 0.3 ×
The dilution of Danieau buffer) anesthesia, then with scalpel along the direction excision zebra fish juvenile fish notochord tail end vertical with notochord
Part tail fin.
(4) juvenile fish that part tail fin is removed is embedded in 1% low melting-point agarose, is placed in 60mm culture dish, and
The covering of 0.3 × Danieau of 10ml buffer is added on surface, is placed in 28.5 °C.
(5) it after 20 minutes, 2,4,8 hours, is taken pictures with 6000 microscope of Leica DMI, photo is led after tail fin excision
Enter Fiji software and the neutrophil leucocyte of wound area is counted.
As a result as shown in figure 3, after zebra fish juvenile fish is soaked for a period of time in the spirulina gamma-Linolenic acid of 100mg/L,
Its tail fin be wound after initial stage, neutrophil leucocyte quantity is higher than control group, and after tail fin is wound 2 hours, neutrophil leucocyte
Quantity then declines rapidly.
Therefore, it shows the zebra fish juvenile fish through 100mg/L spirulina gamma-Linolenic acid immersion treatment, has in early stage of being wound
Stronger anti-inflammatory ability, and there is antiallergy ability in stage.
The present invention obtains following research conclusion, it may be assumed that by organism (in this application mainly include human body, terrestrial animal,
Aquatic animal) wound directly contact or impregnate with certain density spirulina gamma-Linolenic acid solution, organism wound can be accelerated
Reparation.In addition, if with the spirulina gamma-Linolenic acid feeding of a certain concentration or dosage or raising organism for a period of time after, can
Reinforce the following ability of the organism: understanding that nearby the ability of active oxygen, wound generate the anti-inflammatory power of early stage to wound, wound generates
The antiallergy ability in later period.
Since the spirulina gamma-Linolenic acid of known a certain concentration (50-100g/L) has said effect, inevitable deducibility is worked as
Spirulina gamma-Linolenic acid be fabricated to other products, such as solid food, solid feed, smear cosmetics when, if control is appropriate
Additive amount, it may have similar bioactivity.But it is limited in view of experiment condition, not yet external application smear is being made in determination completely
Should be controlled when cosmetics, solid food, solid feed spirulina gamma-Linolenic acid additive amount why.But speech and in short, the present invention
The spirulina gamma-Linolenic acid for being disclosed a certain concentration or dosage is accelerating regeneration, is promoting wound reparation, scavenging capacity
Oxygen, the bioactivity of anti-inflammatory and antiallergy etc..
Accordingly, those skilled in the technology concerned are done according to the application guidance, such as apply spirulina gamma-Linolenic acid
Wound, antiallergy, paste, creme, lotion, soaking agent anti-inflammatory or that remove oxidant are repaired in production, for improving body certainly
Body is anti-inflammatory, antiallergy, the food of antioxidant capacity or feed etc., should all be included within the scope of the present invention.
Claims (10)
1. spirulina gamma-Linolenic acid prepare wound reparation, the purposes in anti-inflammatory or antiallergy product.
2. purposes according to claim 1, which is characterized in that the product is zebra fish wound preparation for repairing.
3. purposes according to claim 1, which is characterized in that the product behaviour wound reparation, anti-inflammatory or antiallergy
Product.
4. wanting the described in any item purposes of 1-3 according to right, which is characterized in that the product is drug, cosmetics, food or feeding
Material.
5. according to right want 4 described in purposes, which is characterized in that the product be externally applied product.
6. according to right want 5 described in purposes, the product be external preparation, the preparation be lotion, soaking agent, spray, cream
Agent or creme.
7. according to right want 6 described in purposes, the preparation is soaking agent, and in the soaking agent, spirulina gamma-Linolenic acid
Concentration be 50mg/L ~ 100mg/L；More preferably 100mg/L.
8. according to right want 4 described in purposes, which is characterized in that the product be food or feed.
9. according to right want 8 described in purposes, which is characterized in that the food or feed are liquid product, are produced in the liquid
In product, the concentration of spirulina gamma-Linolenic acid is 100mg/L.
10. wanting the described in any item purposes of 1-9 according to right, it is characterised in that:
Spirulina gamma-Linolenic acid directly contacts with wound or wound, has within the scope of the solution concentration of 50mg/L ~ 100mg/L
Accelerate the effect of wound tissue repair；
Zebra fish juvenile fish is impregnated with the solution of the spirulina gamma-Linolenic acid containing 100mg/L, fish body can be improved and removed after being wound
The ability of active oxygen near its wound；
Zebra fish juvenile fish is impregnated with the solution of the spirulina gamma-Linolenic acid containing 100mg/L, it is anti-after being wound that fish body can be improved
Scorching and antiallergy ability.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910215558.4A CN109893520A (en) | 2019-03-21 | 2019-03-21 | The new application of spirulina gamma-Linolenic acid |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1168104A (en) * | 1995-01-18 | 1997-12-17 | 大正制药株式会社 | Remedy for dermatitis |
| CN103193694A (en) * | 2013-04-27 | 2013-07-10 | 云南蓝钻生物科技有限公司 | Method for extracting beta carotene and gamma-linolenic acid from spirulina |
| CN106714792A (en) * | 2014-08-08 | 2017-05-24 | R·米利亚乔 | Mixtures of fatty acids and palmitoylethanolamide for the treatment of inflammatory and allergic pathologies |
| CN108358772A (en) * | 2017-12-20 | 2018-08-03 | 湖北工业大学 | A kind of preparation method and applications of spirulina gamma-Linolenic acid extract |
-
2019
- 2019-03-21 CN CN201910215558.4A patent/CN109893520A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1168104A (en) * | 1995-01-18 | 1997-12-17 | 大正制药株式会社 | Remedy for dermatitis |
| CN103193694A (en) * | 2013-04-27 | 2013-07-10 | 云南蓝钻生物科技有限公司 | Method for extracting beta carotene and gamma-linolenic acid from spirulina |
| CN106714792A (en) * | 2014-08-08 | 2017-05-24 | R·米利亚乔 | Mixtures of fatty acids and palmitoylethanolamide for the treatment of inflammatory and allergic pathologies |
| CN108358772A (en) * | 2017-12-20 | 2018-08-03 | 湖北工业大学 | A kind of preparation method and applications of spirulina gamma-Linolenic acid extract |
Non-Patent Citations (1)
| Title |
|---|
| DEREK J. RUTHIG等: "Both (n-3) and (n-6) Fatty Acids Stimulate Wound Healing in the Rat Intestinal Epithelial Cell Line, IEC-6", 《J NUTR.》 * |
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