CN109884154A - One kind being based on Fe2+-H2O2The electrochemical sensing assays method of o-phenylenediamine system - Google Patents
One kind being based on Fe2+-H2O2The electrochemical sensing assays method of o-phenylenediamine system Download PDFInfo
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- CN109884154A CN109884154A CN201910170782.6A CN201910170782A CN109884154A CN 109884154 A CN109884154 A CN 109884154A CN 201910170782 A CN201910170782 A CN 201910170782A CN 109884154 A CN109884154 A CN 109884154A
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- glucose
- phenylenediamine
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Abstract
The invention discloses one kind to be based on Fe2+‑H2O2The electrochemical sensing assays method of o-phenylenediamine system, belongs to electrochemica biological analysis technical field.Object glucose, glucose oxidase are mixed and are incubated for；The reaction solution of glucose and glucose oxidase is added in HCl-NaAc buffer and solution of ferrous chloride；O-phenylenediamine solution is added, quantitative detection is carried out to mixed liquor using Differential Pulse Voltammetry.Operation of the present invention is simple, and without cumbersome electrode modification and cleaning process, detection method simplicity, quick, high sensitivity provide a kind of simple, cheap, quick, highly sensitive and stable performance new detecting method for clinical biochemical Molecular Detection.Compared with traditional Electrochemical Detection glucose, due to Fe2+‑H2O2O-phenylenediamine, which can produce, has very strong electrochemical response, so that it greatly improves the sensitivity of glucose detection.
Description
Technical field
The invention belongs to electrochemica biological analysis technical fields, more particularly to one kind to be based on Fe2+-H2O2O-phenylenediamine body
The electrochemical sensing assays method of system.
Background technique
Currently, the prior art commonly used in the trade is such that
Glucose detection plays an important role in the various fields from biomedical applications to ecological approach.Grape
Sugar is the important indicator of human body, especially to the assessment of diabetes.Therefore, physiology of the measurement of blood sugar concentration for monitoring human body
Situation is most important.The World Health Organization (WHO) and American Diabetes Association (ADA) receiving are easier, less expensive, repeat,
Sensitive and cost-effective glucose test method.On this basis, it has developed various based on unlike signal
The method and strategy of production principle are used for the detection of concentration of glucose, for example, being based on chemiluminescent method, fluorescence-based side
Method, the method based on colorimetric detection.But these most of methods are due to needing the scientific instrument of complex and expensive and the behaviour of profession
Make personnel, so limiting further applying for they.Electrochemical detection method is due to its low cost, quickly, high
Sensitivity and it is easy to the advantages that minimizing, is expected to be used for exempt from the foundation of the analysis method of expensive instrument, obtains rapidly
Development and application, gradually become one of most widely used bioanalytical method, are related to the every field of Biological Detection.Mesh
Before, electrochemical method, especially current measuring method are widely used to the sensing analysis of glucose.However, these are used for Portugal
The electrochemistry strategy of grape sugar detection is mostly based on heterogeneous assays, needs to carry out multi step modification and cleaning, process to electrode surface
It is cumbersome and time-consuming.And homogeneous determination has many advantages, such as easy to operate, and response quickly and reaction efficiency are high, and it is therefore, developing faster, more
Simply it is extremely important without immobilization electrochemical sensing method for detecting glucose.
Ferrous ion (Fe2+) it is one of ionic species of ferro element, it is the important gold in many confactors of native enzyme
Belong to ion, such as peroxidase, cytochrome oxidase and dehydrogenase.Key effect is played in enzymic catalytic reaction.As
Active transition metal ion, Fe2+Some chemical reactions can also be catalyzed, as Fenton reacts.Fenton reagent has very strong
Oxidation susceptibility.When ferrous ion is by H2O2When being oxidized to ferric ion, the hydroxyl free of hydroxide ion and high activity can be generated
Base.Since the oxidation susceptibility of Fenton reagent is strong, have received widespread attention.In recent years, Fenton reagent is used for a variety of ratios
To detect various analytes in colour sensor and fluorescent optical sensor, for the method for the highly sensitive Electrochemical Detection glucose of development
The open visual field newly, shows very promising application prospect in terms of bioanalysis.Fe2+As catalyst, can urge
Change H2O2It aoxidizes o-phenylenediamine (OPD) and generates 2, the 2 '-aminoazabenzols (DAP) with electro-chemical activity, can be used as electrochemistry
Signal designation agent.This unique oxidation susceptibility of Fenton reagent is very beneficial for building for detecting glucose high sensitivity nothing
The electrochemical sensor of immobilization.Therefore, Fe is utilized2+-H2O2O-phenylenediamine system carries out opening for electrochemical sensing assays method
Hair has great practical significance.
In conclusion problem of the existing technology is:
(1) method for detecting glucose, such as chemoluminescence method, fluorescence method, colorimetric method, these methods need complicated high
Expensive scientific instrument and professional operator, and be not easy to minimize.
(2) heterogeneous assays are mostly based on currently used for the electrochemistry strategy of glucose detection, need to electrode surface into
Row multi step modification and cleaning, process are cumbersome and time-consuming.
(3) selectivity of most of electrochemical glucose sensor is poor, because in the glycoxidative potential range of grape,
Some other sugar and some endogenous chaff interferents can be also oxidized.And sensitivity is low, detection range is narrow.
Solve the difficulty of above-mentioned technical problem: a kind of novel highly sensitive, highly selective glucose electricity without immobilization
The building of chemical sensor.
Solve the meaning of above-mentioned technical problem: open a kind of based on Fe2+-H2O2O-phenylenediamine system is equal for electrochemistry
Mutually detect the method for glucose.Compared with traditional glucose sensor, which obviate the foundation of the analysis method of expensive instrument；
The time of electrochemical response is shortened using homogeneous detection and avoids cumbersome electrode modification and cleaning process.Portugal of the invention
Grape sugar test method is easier, sensitiveer, selectivity and cost-effectiveness is higher, and is easy to minimize.For the inspection of clinical biochemical molecule
It surveys and provides a kind of simple, cheap, quick, highly sensitive and stable performance new detecting method.
Summary of the invention
In view of the problems of the existing technology, the present invention provides one kind to be based on Fe2+-H2O2The electricity of o-phenylenediamine system
Chemical sensitisation analysis method.
The invention is realized in this way a kind of be based on Fe2+-H2O2The electrochemical sensing assays method of o-phenylenediamine system,
Specifically includes the following steps:
Step 1: object glucose, glucose oxidase are mixed and are incubated for；
Step 2: the reaction solution of glucose and glucose oxidase is added to HCl-NaAc buffer and frerrous chloride is molten
In liquid；
Step 3: being added o-phenylenediamine solution, carries out quantitative detection to mixed liquor using Differential Pulse Voltammetry.
Further, in step 1, incubation temperature is 37 DEG C, time 1h.Object glucose and glucose oxidase
Reaction temperature and time control, are conducive to the two fully reacting, the detection error for avoiding unreacted completely caused.
Further, in step 1, the concentration of glucose oxidase is 1mg/ml, and dosage is 13 μ L.Glucose oxidase
Concentration and dosage control, guarantee to object it is complete be catalyzed and without waste.
Further, in step 1, different glucose dosage is 27 μ L.Under the premise of realizing detection, reach micro
The purpose of detection realizes the Non-destructive test to target sample.
Further, in step 2, the concentration of frerrous chloride is 10mM, and dosage is 20 μ L.The control of iron concentration and dosage
System guarantees electrochemical response signal peak and without waste (see Fig. 5).
Further, in step 2, buffer is 0.1M HCl-NaAc, and pH 3.0, dosage is 80 μ L.Detect bottom liquid pH control
System is also to obtain response signal peak value (see Fig. 6).
Further, in step 3, the concentration of o-phenylenediamine is 18mM, and dosage is 200 μ L.O-phenylenediamine concentration and dosage control
System guarantees signal acquisition and amplification effect (see Fig. 7).
Further, in step 3, the scanning potential range of Differential Pulse Voltammetry is 0.1V~-0.7V.It is rung in this method
It is near -0.3V, in order to more preferably show that response signal peak will scan potential range extension ± 0.4V that induction signal, which goes out peak position,.
It is of the invention based on Fe2+-H2O2The measuring principle of the glucose electrochemical sensing assays method of o-phenylenediamine system
Be: initially, glucose is converted to gluconic acid by glucose oxidase, while generating H2O2.The H of formation2O2With ferrous ion
(Fe2+) reaction, the hydroxyl radical free radical (OH) of high activity is generated, the further catalysis oxidation o-phenylenediamine (OPD) of OH generates tool
There are forceful electric power chemically active 2,2 '-aminoazabenzols (DAP), color becomes yellow from colourless.With the increasing of concentration of glucose
Add, generating, there is the amount of 2,2 '-aminoazabenzols of electro-chemical activity constantly to increase, and DPV signal response intensity also just constantly increases
By force, to achieve the purpose that quantitative detection glucose.
In conclusion advantages of the present invention and good effect are as follows:
(1) the present invention provides one kind to be based on Fe2+-H2O2O-phenylenediamine system homogeneously detects glucose for electrochemistry
Method, the electrochemical response time be less than 10s, detection range be 0.1 μM to 10000 μM, than existing electrochemical analysis method
Detection range for detecting glucose is wide (Talanta.2018,182:230-240), and in common interference object such as ascorbic acid
(AA), in the presence of dopamine (DA), uric acid (UA), glutamic acid (Glu E), to glucose all have good selectivity (see
Fig. 4).
(2) it is detected with other electrochemical analysis methods (J Colloid Interface Sci.2018,511:355-64)
Glucose is compared, due to Fe2+-H2O2O-phenylenediamine, which can produce, has very strong electrochemical response, so that it is to glucose
The sensitivity of detection greatly improves, and detection limit has reached 0.15 μM.
(3) present invention is closely related with biocatalytic reaction.By utilizing Fe2+-H2O2The electrochemistry of o-phenylenediamine system
Sensing analytical method cannot be only used for the quantitative detection of glucose, can be extended to a series of hypersensitivities of analysis and special
Property biomarker/corresponding oxidizing ferment, such as uric acid (UA)/urate oxidase (UOx), sarcosine (SO)/sarcosine oxidation
Enzyme (SOx), choline (CO)/choline oxidase (COx), lactic acid (LA)/lactate oxidase (LOx), xanthine (XO)/xanthine oxidase
Change objects such as enzyme (XOx).
The present invention carries out the quantitative inspection of high sensitivity by electrochemical sensing Differential Pulse Voltammetry to the concentration of glucose
It surveys, is related to homogeneous Electrochemical Detection, easy to operate, without cumbersome electrode modification and cleaning process, detection method is easy, fast
Speed, detection range be wide, sensitivity and selectivity are high, and it is simple, cheap, quick, high to provide one kind for clinical biochemical Molecular Detection
Sensitive and stable performance new detecting method.
Detailed description of the invention
Fig. 1 is provided in an embodiment of the present invention based on Fe2+-H2O2The glucose electrochemical sensing of o-phenylenediamine system point
Analyse method flow diagram.
Fig. 2 is provided in an embodiment of the present invention based on Fe2+-H2O2The glucose electrochemical sensing of o-phenylenediamine system point
Analyse Method And Principle schematic diagram.
Fig. 3 is provided in an embodiment of the present invention based on Fe2+-H2O2The glucose analysis method electrification of o-phenylenediamine system
Learn the result schematic diagram of detection glucose.
Fig. 4 is provided in an embodiment of the present invention based on Fe2+-H2O2The glucose analysis method electrification of o-phenylenediamine system
Learn the selective experimental result schematic diagram of detection glucose.
Fig. 5 is provided in an embodiment of the present invention based on Fe2+-H2O2The glucose analysis method electrification of o-phenylenediamine system
Learn the iron concentration optimum results schematic diagram of detection glucose.
Fig. 6 is provided in an embodiment of the present invention based on Fe2+-H2O2The glucose analysis method electrification of o-phenylenediamine system
Learn the pH optimum results schematic diagram of detection glucose.
Fig. 7 is provided in an embodiment of the present invention based on Fe2+-H2O2The glucose analysis method electrification of o-phenylenediamine system
Learn the o-phenylenediamine concentration optimization result schematic diagram of detection glucose.
Fig. 8 is provided in an embodiment of the present invention based on Fe2+-H2O2The glucose analysis method electrification of o-phenylenediamine system
Iron ion-the o-phenylenediamine for learning detection glucose enhances signal response results schematic diagram altogether.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
The purpose of the present invention is to provide one kind to be based on Fe2+-H2O2The electrochemical sensing assays side of o-phenylenediamine system
Method, by taking glucose detection as an example；Due to Fe2+It is catalyzed H2O2It aoxidizes o-phenylenediamine and generates the 2,2 '-amino with electro-chemical activity
After azobenzene, redox peak current is generated in electrode surface, causes current responsing signal size with H2O2The variation of concentration
And change；Using this feature, it is stable based on Fe to can establish a kind of simple, cheap, quick, sensitive and performance2+-H2O2-
The new method of the glucose electrochemical sensing assays of o-phenylenediamine system.
It elaborates with reference to the accompanying drawing to application principle of the invention；
As shown in Figure 1, provided in an embodiment of the present invention be based on Fe2+-H2O2The electrochemical sensing assays of o-phenylenediamine system
Method, specifically includes the following steps:
S101: object glucose, glucose oxidase are mixed and are incubated for；
S102: the reaction solution of glucose and glucose oxidase is added to HCl-NaAc buffer and solution of ferrous chloride
In；
S103: being added o-phenylenediamine solution, carries out quantitative detection to mixed liquor using Differential Pulse Voltammetry.
In step S101, incubation temperature provided in an embodiment of the present invention is 37 DEG C, time 1h.
In step S101, the concentration of glucose oxidase provided in an embodiment of the present invention is 1mg/ml, and dosage is 13 μ L.
In step S101, different glucose dosage provided in an embodiment of the present invention is 27 μ L.
In step S102, the concentration of frerrous chloride provided in an embodiment of the present invention is 10mM, and dosage is 20 μ L.
In step S102, buffer provided in an embodiment of the present invention is 0.1M HCl-NaAc, and pH 3.0, dosage is 80 μ
L。
In step S103, the concentration of o-phenylenediamine provided in an embodiment of the present invention is 18mM, and dosage is 200 μ L.
In step S103, the scanning potential range of Differential Pulse Voltammetry provided in an embodiment of the present invention be 0.1V~-
0.7V。
It is provided in an embodiment of the present invention to be based on Fe2+-H2O2The glucose electrochemical sensing assays method of o-phenylenediamine system
Measuring principle:
Glucose oxidase catalysis hydrolyzation of glucose generates gluconic acid and H2O2, the H of formation2O2With ferrous ion (Fe2+)
Reaction generates the hydroxyl radical free radical (OH) of high activity, and the further catalysis oxidation o-phenylenediamine (OPD) of OH, which generates, has forceful electric power
Chemically active 2,2 '-aminoazabenzols (DAP), color becomes yellow from colourless；With the increase of concentration of glucose, generate
2 with electro-chemical activity, the amount of 2 '-aminoazabenzols constantly increases, Differential Pulse Voltammetry (DPV) signal response intensity
Just constantly enhancing, to achieve the purpose that quantitative detection glucose.
Application principle of the invention is further described combined with specific embodiments below；
Embodiment 1；One kind being based on Fe2+-H2O2The electrochemical sensing assays method of o-phenylenediamine system, to detect grape
For sugar；
Based on Fe2+-H2O2The electrochemical sensing assays method of o-phenylenediamine system homogeneously detects glucose；
A series of Glucose standards sample of 27 concentration of μ L is mixed with the 1mg/mL glucose oxidase solution of 13 μ L respectively
Afterwards, it is incubated for 1 hour at 37 DEG C.It is resulting to contain H2O2Reaction solution be added containing 80 μ L, 0.1M HCl-NaAc (pH 3.0),
20 μ L, 10mM FeCl2Mixed liquor in, be eventually adding 200 μ L, 10mM OPD and this then recorded by electrochemical workstation
The differential pulse voltammetry voltammetric current of a little solution responds variation, the relationship changed according to the size of current responsing signal and concentration of glucose
Draw standard working curve.
As shown in Fig. 2, provided in an embodiment of the present invention be based on Fe2+-H2O2The glucose electrochemistry of o-phenylenediamine system passes
Feel analysis method schematic illustration.
As shown in figure 3, provided in an embodiment of the present invention be based on Fe2+-H2O2The glucose analysis method of o-phenylenediamine system
The result schematic diagram of Electrochemical Detection glucose.
As shown in figure 4, provided in an embodiment of the present invention be based on Fe2+-H2O2The glucose analysis method of o-phenylenediamine system
The selective experimental result schematic diagram of Electrochemical Detection glucose.
As shown in figure 5, provided in an embodiment of the present invention be based on Fe2+-H2O2The glucose analysis method of o-phenylenediamine system
The iron concentration optimum results schematic diagram of Electrochemical Detection glucose.
As shown in fig. 6, provided in an embodiment of the present invention be based on Fe2+-H2O2The glucose analysis method of o-phenylenediamine system
The pH optimum results schematic diagram of Electrochemical Detection glucose.
As shown in fig. 7, provided in an embodiment of the present invention be based on Fe2+-H2O2The glucose analysis method of o-phenylenediamine system
The o-phenylenediamine concentration optimization result schematic diagram of Electrochemical Detection glucose.
As shown in figure 8, provided in an embodiment of the present invention be based on Fe2+-H2O2The glucose analysis method of o-phenylenediamine system
Iron ion-o-phenylenediamine of Electrochemical Detection glucose enhances signal response results schematic diagram altogether.
In figure, (A) DPV response curve；(B) standard working curve figure.
The present invention can carry out determining for high sensitivity by concentration of the electrochemical sensing Differential Pulse Voltammetry to glucose
Amount detection, with the increase of concentration of glucose, current responsing signal constantly increases, and is limited to 0.15 μM to the detection of glucose, line
Property range is 0.1 μM~10000 μM.It is easy to operate the present invention relates to homogeneous Electrochemical Detection, without cumbersome electrode modification and
Cleaning process, detection method simplicity, quick, high sensitivity provide a kind of simple, cheap, fast for clinical biochemical Molecular Detection
Fast, highly sensitive and stable performance new detecting method.Experimental data is shown in Fig. 3-Fig. 8.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (8)
1. one kind is based on Fe2+-H2O2The electrochemical sensing assays method of o-phenylenediamine system, which is characterized in that it is described based on
Fe2+-H2O2The electrochemical sensing assays method of o-phenylenediamine system, specifically includes the following steps:
Step 1: object glucose, glucose oxidase are mixed and are incubated for；
Step 2: the reaction solution of glucose and glucose oxidase is added to HCl-NaAc buffer and solution of ferrous chloride
In；
Step 3: being added o-phenylenediamine solution, carries out quantitative detection to mixed liquor using Differential Pulse Voltammetry.
2. being based on Fe as described in claim 12+-H2O2The electrochemical sensing assays method of o-phenylenediamine system, feature exist
In in the step 1, incubation temperature is 37 DEG C, time 1h.
3. being based on Fe as described in claim 12+-H2O2The electrochemical sensing assays method of o-phenylenediamine system, feature exist
In in the step 1, the concentration of glucose oxidase is 1mg/ml, and dosage is 13 μ L.
4. being based on Fe as described in claim 12+-H2O2The electrochemical sensing assays method of o-phenylenediamine system, feature exist
In in the step 1, different glucose dosage is 27 μ L.
5. being based on Fe as described in claim 12+-H2O2The electrochemical sensing assays method of o-phenylenediamine system, feature exist
In in the step 2, the concentration of frerrous chloride is 10mM, and dosage is 20 μ L.
6. being based on Fe as described in claim 12+-H2O2The electrochemical sensing assays method of o-phenylenediamine system, feature exist
In in the step 2, HCl-NaAc buffer is 0.1M HCl-NaAc, and pH 3.0, dosage is 80 μ L.
7. being based on Fe as described in claim 12+-H2O2The electrochemical sensing assays method of o-phenylenediamine system, feature exist
In in the step 3, the concentration of o-phenylenediamine is 18mM, and dosage is 200 μ L.
8. being based on Fe as described in claim 12+-H2O2The electrochemical sensing assays method of o-phenylenediamine system, feature exist
In in the step 3, the scanning potential range of Differential Pulse Voltammetry is 0.1V~-0.7V.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1497256A (en) * | 2002-09-19 | 2004-05-19 | 王湘生 | Non-invasive method for monitoring blood sugar and its device |
| CN2783321Y (en) * | 2002-09-19 | 2006-05-24 | 王湘生 | Wound free blood sugar monitoring device |
| CN102654475A (en) * | 2012-03-23 | 2012-09-05 | 上海大学 | Bioelectrochemical sensor used for detecting hydrogen peroxide and manufacturing method thereof |
-
2019
- 2019-03-07 CN CN201910170782.6A patent/CN109884154A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1497256A (en) * | 2002-09-19 | 2004-05-19 | 王湘生 | Non-invasive method for monitoring blood sugar and its device |
| CN2783321Y (en) * | 2002-09-19 | 2006-05-24 | 王湘生 | Wound free blood sugar monitoring device |
| CN102654475A (en) * | 2012-03-23 | 2012-09-05 | 上海大学 | Bioelectrochemical sensor used for detecting hydrogen peroxide and manufacturing method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 亢晓峰 等: "极谱免疫法测定抗血清中溶血素的活性", 《分析化学》 * |
| 何亚楠 等: "邻苯二胺一过氧化氢一辣根过氧化物酶酶联免疫示差脉冲伏安法测定人血清类风湿因子抗体", 《分析化学》 * |
| 梁爱惠等: "Fe2+-邻苯二胺- H2O2催化体系分光光度法测定痕量过氧化氢", 《桂林工学院学报》 * |
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