CN109884012A - Fluorescent microsphere testing piece, its production method and production component - Google Patents
Fluorescent microsphere testing piece, its production method and production component Download PDFInfo
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- CN109884012A CN109884012A CN201910160304.7A CN201910160304A CN109884012A CN 109884012 A CN109884012 A CN 109884012A CN 201910160304 A CN201910160304 A CN 201910160304A CN 109884012 A CN109884012 A CN 109884012A
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- fluorescent microsphere
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Abstract
The production method of present invention offer fluorescent microsphere testing piece, including making raw material accommodating area, fluorescent microsphere solution is instilled into the natural air-dried fluorescent microsphere for forming one layer of tiling of levelling in raw material accommodating area, resin is added dropwise on the fluorescent microsphere, covered fills full resin tight so that raw material accommodating area is formed in encapsulated space, and fluorescent microsphere testing piece can be obtained in removing coverslip after solidified resin.By making fluorescent microsphere solution instill the natural air-dried fluorescent microsphere for forming one layer of tiling of levelling in raw material accommodating area in this method, to ensure that fluorescent microsphere is not laminated in any longitudinal arrangement, does not accumulate, and then accurate testing result can get；Controlled as measuring scale it is final made from fluorescent microsphere testing piece thickness, so as to obtain the fluorescent microsphere testing piece of different-thickness according to demand.
Description
Technical field
The present invention relates to the technical field of performance test of confocal endoscope, survey in particular to a kind of fluorescent microsphere
Test piece, its production method and production component.
Background technique
Fluorescent microsphere testing piece has very extensive purposes in the integrated detection field of confocal endoscope, including is used for
Inspection and the inspection of fluorescent staining cell of longitudinal resolution etc., however the structure of current fluorescent microsphere testing piece includes
Glass slide, coverslip and fluorescent microsphere between the two, the fluorescent microsphere of this structure have the defects that it is certain, to detect axial direction
For resolution ratio, on the one hand due to being detected when need to obtain the grey scale change of fluorescent microsphere along longitudinal direction, need fluorescence in this way
Microballoon is not laminated in any longitudinal arrangement, does not accumulate, but fluorescent microsphere is at random in existing fluorescent microsphere testing piece is distributed in
In glass slide and coverslip, it is cumbersome to will lead to the satisfactory fluorescent microsphere testing piece of production in this way；On the other hand due to packet
Include coverslip, and coverslip with a thickness of 170um so that the thickness of entire fluorescent microsphere testing piece is greater than 170um, for
Working distance is less than the confocal endoscope of 170um, if coverslip cannot be made to cover using the fluorescent microsphere testing piece of this kind of structure
Fluorescent microsphere imaging under lid, cannot also detect axial resolution certainly.
Therefore it provides a kind of, to prepare simple, the accurate fluorescent microsphere testing piece of testing result be problem to be solved.
Summary of the invention
It is an object of the invention to solve at least one technical problem existing in the prior art, fluorescent microsphere test is provided
Fluorescent microsphere solution is instilled natural levelling in raw material accommodating area and air-dries shape by the production method of piece, including production raw material accommodating area
At one layer of fluorescent microsphere of tiling, resin is added dropwise on the fluorescent microsphere, covered is so that raw material accommodating area forms envelope
It fills and fills full resin tight in space, glimmering big gun microballoon testing piece can be obtained in removing coverslip after solidified resin.In this method
By making fluorescent microsphere solution instill the natural air-dried fluorescent microsphere for forming one layer of tiling of levelling in raw material accommodating area, to guarantee
Fluorescent microsphere is not laminated in any longitudinal arrangement, does not accumulate, and then can get accurate testing result；Pass through measuring scale
The thickness of final obtained fluorescent microsphere testing piece is controlled, so as to obtaining the fluorescent microsphere test of different-thickness according to demand
Piece.
It is of the invention the specific scheme is that
A kind of production method of fluorescent microsphere testing piece, comprising:
Two or more measuring scale interval horizontal are formed on the glass slide raw material and held by S1 horizontal glass slide
Set area；
Fluorescent microsphere solution is instilled the raw material accommodating area in the step S1 by S2, allows the fluorescent microsphere solution of instillation
Natural levelling is so that the fluorescent microsphere in the fluorescent microsphere solution is laid into one layer；
S3 air-dries the fluorescent microsphere solution in the step S2 to obtain fluorescent microsphere；
Resin is added dropwise to the raw material accommodating area on the fluorescent microsphere that the step S3 is obtained in S4, is then covered with lid glass
Piece, so as to fill to obtain tight and the fluorescent microsphere quilt in the enclosure space that the glass slide, measuring scale and coverslip are formed
It is fully wrapped around；
S5 solidifies the resin in the step S4 in enclosure space；
S6 removes the coverslip on the resin after solidifying in the step S5, includes resin, fluorescent microsphere layer to be formed
And the fluorescent microsphere testing piece of glass slide.
It can get predetermined thickness through the above steps, fluorescent microsphere is not laminated in any longitudinal arrangement, it is glimmering not accumulate
Light microballoon testing piece.
The present invention also provides a kind of fluorescent microsphere testing pieces, are made by above-mentioned production method, including glass slide, resin
And fluorescent microsphere, the resin are set on the glass slide, the fluorescent microsphere is embedded in the resin so that the resin
The fully wrapped around fluorescent microsphere, the fluorescent microsphere and the glass slide are tangent.
The present invention provides a kind of production component for making above-mentioned fluorescent microsphere testing piece again, comprising:
Raw material accommodating area forms component, including glass slide, measuring scale, the measuring scale are vertically installed in the glass slide
On, the measuring scale is spaced and parallel to be provided with 2 or more to form raw material accommodating area；
Raw material, fluorescent microsphere solution, resin, the fluorescent microsphere solution for instill in the raw material accommodating area air-dry after
The fluorescent microsphere of single layer is formed on the glass slide, the resin, which is used to instill the raw material above the fluorescent microsphere, to be held
It sets in area；Coverslip is set on the measuring scale, so that the enclosure space that the glass slide, measuring scale and coverslip are formed
Interior resin fill tight and the fluorescent microsphere are completely wrapped；
Solidification equipment, for solidifying the resin in the enclosure space；
Stripping off device, for removing the coverslip on the resin after solidifying.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment attached
Figure is briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not construed as pair
The restriction of range for those of ordinary skill in the art without creative efforts, can also be according to this
A little attached drawings obtain other relevant attached drawings.
Fig. 1 is a kind of flow chart of the production method of fluorescent microsphere testing piece provided by the invention；
Fig. 2 is a kind of cooperation schematic diagram of the production component of fluorescent microsphere testing piece provided by the invention；
Fig. 3 is a kind of structural schematic diagram of fluorescent microsphere testing piece provided by the invention；
Fig. 4 is that the fluorescence for a certain longitudinal cross-section for carrying out axial resolution test using the fluorescent microsphere testing piece of Fig. 3 is micro-
Ball image；
Fig. 5 is the axial resolution data obtained using the fluorescent microsphere testing piece of Fig. 3.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is
A part of the embodiment of the present invention, instead of all the embodiments.The present invention being usually described and illustrated herein in the accompanying drawings is implemented
The component of example can be arranged and be designed with a variety of different configurations.
Therefore, the detailed description of the embodiment of the present invention provided in the accompanying drawings is not intended to limit below claimed
The scope of the present invention, but be merely representative of selected embodiment of the invention.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without creative efforts belongs to the model that the present invention protects
It encloses.
It should also be noted that similar label and letter indicate similar terms in following attached drawing, therefore, once a certain Xiang Yi
It is defined in a attached drawing, does not then need that it is further defined and explained in subsequent attached drawing.
In the description of the present invention, it should be noted that term " in ", "upper", "lower", "left", "right", "inner", "outside"
The orientation or positional relationship of equal instructions is to be based on the orientation or positional relationship shown in the drawings or when invention product uses is used
The orientation or positional relationship often put, is merely for convenience of description of the present invention and simplification of the description, rather than indication or suggestion is signified
Device or element must have a particular orientation, be constructed and operated in a specific orientation, therefore should not be understood as to the present invention
Limitation.In addition, term " first ", " second ", " third " etc. are only used for distinguishing description, it is not understood to indicate or imply phase
To importance.
In addition, the terms such as term "horizontal", "vertical", " pendency " are not offered as requiring component abswolute level or pendency, and
It is that can be slightly tilted.It is not to indicate the structure if "horizontal" only refers to that its direction is more horizontal with respect to for "vertical"
It has to fully horizontally, but can be slightly tilted.
In the description of the present invention, it is also necessary to which explanation is unless specifically defined or limited otherwise, term " setting ",
" installation ", " connected ", " connection " shall be understood in a broad sense, for example, it may be fixedly connected, may be a detachable connection or one
Connect to body；It can be mechanical connection, be also possible to be electrically connected；It can be directly connected, it can also be indirect by intermediary
It is connected, can be the connection inside two elements.For the ordinary skill in the art, on being understood with concrete condition
State the concrete meaning of term in the present invention.
As shown in Figure 1, the present invention provides a kind of production method of fluorescent microsphere testing piece, comprising:
Two or more measuring scale interval horizontal are formed on the glass slide raw material and held by S1 horizontal glass slide
Set area；
Fluorescent microsphere solution is instilled the raw material accommodating area in the step S1 by S2, allows the fluorescent microsphere solution of instillation
Natural levelling is so that the fluorescent microsphere in the fluorescent microsphere solution is laid into one layer；
S3 air-dries the fluorescent microsphere solution in the step S2 to obtain fluorescent microsphere；
Resin is added dropwise to the raw material accommodating area on the fluorescent microsphere that the step S3 is obtained in S4, is then covered with lid glass
Piece, so as to fill to obtain tight and the fluorescent microsphere quilt in the enclosure space that the glass slide, measuring scale and coverslip are formed
It is fully wrapped around；
S5 solidifies the resin in the step S4 in enclosure space；
S6 removes the coverslip on the resin after solidifying in the step S5, includes resin, fluorescent microsphere layer to be formed
And the fluorescent microsphere testing piece of glass slide.
In this method, the raw material accommodating area of glass slide, measuring scale formation predetermined altitude and size is first passed through, then to the original
Expect that accommodating area instills fluorescent microsphere solution, which is laid into one layer with fluorescent microsphere therein after natural levelling and is
Preferably, then air-dry the fluorescent microsphere solution and obtain fluorescent microsphere, then resin is added on the fluorescent microsphere, be added the amount of resin with
Be covered in the enclosure space that raw material accommodating area is formed after coverslip fill tight and the fluorescent microsphere be completely wrapped for
Preferably；Fluorescent microsphere testing piece is obtained after further removing coverslip after solidified resin.This method is not only simple to operation, but also
Ensure that fluorescent microsphere is not laminated in any longitudinal arrangement, does not accumulate, can also be controlled as measuring scale it is final made from
The thickness of fluorescent microsphere testing piece, so as to obtain the fluorescent microsphere testing piece of different-thickness according to demand.
The present embodiment is illustrated by taking the axial resolution for measuring confocal endoscope as an example, further, the step
Measuring scale in S1 will select thickness less than the measuring scale of the working distance of the confocal endoscope, and the present embodiment is due to that will detect
The working distance of confocal endoscope belong to micron order, therefore select micron-sized clearance gauge as measuring scale, the folding of the resin
Penetrating rate range is 1.33-1.40, and the thickness of clearance gauge is less than the working distance of confocal endoscope, so that fluorescent microsphere can be at when measurement
Picture, to guarantee going on smoothly for detection；Resin selects the range, mainly when designing confocal endoscope, can assume that altogether
The object space for focusing objective of endoscope is water, and the refractive index of water is about 1.33, this range can guarantee the testing piece and copolymerization
The miniature object lens operating condition of burnt endoscope distal end matches.
Further, obtain the fluorescent microsphere solution in the step S2 method particularly includes: by solution concentration be 0.3%
Fluorescent microsphere solution dilute 1000-10000 times.When the fluorescent microsphere solution directly bought dense tides over big, need to it
It is diluted, such as matches the InSpeck of Mo Feishier companyTMGreen series.
Further, cured in the step S5 method particularly includes: using uniform ultraviolet source along by glass slide to
The resin is irradiated in the direction of coverslip." being irradiated by the direction of glass slide to coverslip " can make the resin close to glass slide solid
Change is more thorough, and such resin more fastens in conjunction with glass slide, is more easier to separate with coverslip.
Specifically, the fluorescent microsphere testing piece that resin thickness is 40um is finally obtained, then parameter selection: the step S1
In clearance gauge to select the clearance gauge of 40um, the ranges of indices of refraction of the resin is 1.33；The fluorescence for being 0.3% by solution concentration is micro-
Ball solution dilutes 10000 times.
The thickness of above-mentioned clearance gauge, the refractive index of resin can be adjusted as the case may be, corresponding fluorometric investigation piece
Production method and the refractive index of resin be the identical with a thickness of 40um of 1.33, clearance gauge, only modify corresponding resin index or
The thickness parameter of clearance gauge is just.Whether need to dilute and dilute how many times as the concentration of fluorescent microsphere solution, also according to reality
Depending on the situation of border, as long as meeting the fluorescent microsphere solution nature levelling instilled so that fluorescence in the fluorescent microsphere solution
Microballoon is laid into one layer.
As shown in Fig. 2, the present invention also provides a kind of production components for making above-mentioned fluorescent microsphere testing piece, comprising:
Raw material accommodating area forms component, including glass slide 1, measuring scale 2, the measuring scale are vertically installed in the glass slide
On, the measuring scale is spaced and parallel to be provided with 2 or more to form raw material accommodating area；
Raw material, fluorescent microsphere solution 3, resin 4, the fluorescent microsphere solution are air-dried for instilling in the raw material accommodating area
The fluorescent microsphere of single layer is formed on the glass slide afterwards, the resin is used to instill the raw material above the fluorescent microsphere
In accommodating area；
Coverslip 5 is set on the measuring scale, so that the closing that the glass slide, measuring scale and coverslip are formed is empty
In resin fill tight and the fluorescent microsphere are completely wrapped；
Solidification equipment 6, for solidifying the resin in the enclosure space；
Stripping off device, for removing the coverslip on the resin after solidifying.
The working principle of the production component are as follows: component construction raw material accommodating area is formed by raw material accommodating area, raw material is pressed
It according to certain sequence and requires to be added in raw material accommodating area, then covered fills to obtain tight and institute to form resin
It states the enclosure space that fluorescent microsphere is completely wrapped and passes through stripping off device after solidifying finally by solidification equipment to resin
Coverslip is removed.Solidification equipment in this production component can be ultraviolet lamp or other can reach the dress of resin solidification
It sets, stripping off device can be tweezers or other devices for realizing coverslip removing resin.
Further, it since the working distance of the present embodiment confocal endoscope to be detected belongs to micron order, selects
Micron-sized clearance gauge as measuring scale, the clearance gauge with a thickness of 20um-50um, the refractive index of resin is 1.33-1.40；It is described
The concentration of fluorescent microsphere solution is 0.00001%-0.001%, uses the clearance gauge of the thickness range that final fluorescent microsphere is surveyed
The thickness of resin is also in the range in test piece, to guarantee that the fluorescent microsphere testing piece finally obtained can measure working distance and exist
The confocal endoscope of 70-100um；The concentration of the fluorescent microsphere solution is 0.00001%-0.001%, it is ensured that is directly dripped
When adding the fluorescent microsphere solution, fluorescent microsphere therein is not stacked after the solution levelling；Resin selects the range, is mainly setting
When counting confocal endoscope, it can assume that the object space of the object lens of confocal endoscope is water, and the refractive index of water is about 1.33, this
Range can guarantee that the testing piece focuses the miniature object lens operating condition matching of endoscope distal end together.
Further, the solidification equipment is uniform ultraviolet source.Wavelength 365nm, 5 watts of power are selected in the present embodiment
LED ultraviolet lamp, curing time be 3-5 minutes.
It further, further include air-drying device, the air-drying device is baking oven, for air-drying the fluorescent microsphere solution
Obtain fluorescent microsphere.Air-dry using baking oven and can shorten the time for entirely preparing fluorescent microsphere testing piece.
As shown in figure 3, the present invention also provides a kind of fluorescent microsphere testing pieces, production component as shown in Figure 2 is according to Fig. 1 institute
The production method shown is made, including glass slide 1, resin 31 and fluorescent microsphere 21, the resin are set to the glass slide
On, the fluorescent microsphere is embedded in the resin so that the fully wrapped around fluorescent microsphere of the resin, the fluorescent microsphere with
The glass slide is tangent.Since fluorescent microsphere is tangent with glass slide, to ensure that fluorescent microsphere in any longitudinal arrangement not
Stacking is not accumulated.The fluorescent microsphere testing piece is due to doing raw material using resin and fluorescent microsphere, since the performance of the two is more steady
It is fixed, therefore the fluorescent microsphere testing piece can save for a long time.
Further, the refractive index of the resin be 1.33, the resin with a thickness of 40um.The resin with a thickness of
40um, to guarantee that the fluorescent microsphere testing piece finally obtained can measure working distance in the confocal endoscope of 70-100um.
Resin selects the range, mainly when designing confocal endoscope, can assume that the object space of the object lens of confocal endoscope is water,
And the refractive index of water is about 1.33, this range can guarantee that the testing piece focuses the miniature object lens work item of endoscope distal end together
Part matching.
Although the refractive index that the present embodiment only has chosen resin is 1.33, resin makes fluorescent microsphere with a thickness of 40um
Testing piece, but according to actual needs, the refractive index that also can choose resin is 1.4 or 1.37 etc., resin with a thickness of 20um or
30um etc. makes fluorescent microsphere testing piece, and production method and structure are 1.33 with the refractive index of resin, the thickness of resin
It is identical for 40um, only modify corresponding resin index or resin thickness parameter just.
Fig. 4 gives a certain longitudinal cross-section that axial resolution test is carried out using the fluorescent microsphere testing piece in Fig. 3
Fluorescent microsphere image, wherein brighter one piece represent is image formed by fluorescent microsphere, background is image-carrying fiber bundle end face
Image；Fig. 5 is the axial resolution data obtained by a series of fluorescent microsphere image procossing, specially sets the fortune of motor
Then dynamic step pitch (as distance/pixel) 1um obtains the light intensity (i.e. gray value) of fluorescent microsphere every a step pitch to get arriving
The curve for the gray value that abscissa is distance/pixel, ordinate is fluorescent microsphere, the curve negotiating Manual Logos go out full width at half maximum
The axial direction point of the confocal endoscope obtained by the fluorescent microsphere testing piece can be obtained in (can also be with gaussian curve approximation)
Resolution 7um.Fig. 4,5 are from the fluorescent microsphere testing piece in fact demonstrating use with a thickness of 40um, one kind provided by the invention
Fluorescent microsphere testing piece can be used for measuring the axial resolution of confocal endoscope of the working distance less than 100um.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of production method of fluorescent microsphere testing piece characterized by comprising
Two or more measuring scale interval horizontal are formed raw material accommodating by S1 horizontal glass slide on the glass slide
Area；
Fluorescent microsphere solution is instilled the raw material accommodating area in the step S1 by S2, makes the fluorescent microsphere solution of instillation natural
Levelling is so that the fluorescent microsphere in the fluorescent microsphere solution is laid into one layer；
S3 air-dries the fluorescent microsphere solution in the step S2 to obtain fluorescent microsphere；
Resin is added dropwise to the raw material accommodating area on the fluorescent microsphere that the step S3 is obtained in S4, is then covered with coverslip, with
Filled in the enclosure space for forming the glass slide, measuring scale and coverslip tight and the fluorescent microsphere are wrapped completely
It wraps up in；
S5 solidifies the resin in the step S4 in enclosure space；
S6 removes the coverslip on the resin after solidifying in the step S5, includes resin, fluorescent microsphere layer and load to be formed
The fluorescent microsphere testing piece of slide.
2. manufacturing method according to claim 1, which is characterized in that the fluorescent microsphere testing piece is peeped for being copolymerized in coke
The measurement of the axial resolution of mirror, then the measuring scale in the step S1 will select work of the thickness less than the confocal endoscope
Make away from measuring scale, the ranges of indices of refraction of the resin is 1.33-1.40.
3. production method according to claim 2, which is characterized in that obtain the fluorescent microsphere solution in the step S2
Method particularly includes: the fluorescent microsphere solution that solution concentration is 0.3% is diluted 1000-10000 times.
4. production method according to claim 2 or 3, which is characterized in that cured in the step S5 method particularly includes:
The resin is irradiated along by the direction of glass slide to coverslip using uniform ultraviolet source.
5. a kind of fluorescent microsphere testing piece, which is characterized in that be made, including carried by any production method of claim 1-4
Slide, resin and fluorescent microsphere, the resin are set on the glass slide, the fluorescent microsphere be embedded in the resin so that
The fully wrapped around fluorescent microsphere of resin, the fluorescent microsphere and the glass slide are tangent.
6. fluorescent microsphere testing piece according to claim 5, which is characterized in that the ranges of indices of refraction of the resin is
1.33-1.40 the resin with a thickness of 20um-50um.
7. a kind of production component for making fluorescent microsphere testing piece described in claim 5 characterized by comprising
Raw material accommodating area forms component, including glass slide, measuring scale, the measuring scale are vertically installed on the glass slide, institute
State that measuring scale is spaced and parallel to be provided with 2 or more to form raw material accommodating area；
Raw material, fluorescent microsphere solution, resin, the fluorescent microsphere solution for instill in the raw material accommodating area air-dry after in institute
The fluorescent microsphere that single layer is formed on glass slide is stated, the resin is used to instill the raw material accommodating area above the fluorescent microsphere
It is interior；
Coverslip is set on the measuring scale, so that in the enclosure space that the glass slide, measuring scale and coverslip are formed
Resin fill tight and the fluorescent microsphere are completely wrapped；
Solidification equipment, for solidifying the resin in the enclosure space；
Stripping off device, for removing the coverslip on the resin after solidifying.
8. production component according to claim 6, which is characterized in that the measuring scale with a thickness of 20um-50um, resin
Refractive index be；The concentration of the fluorescent microsphere solution is 0.00001%-0.001%.
9. production component according to claim 6, which is characterized in that the solidification equipment is uniform ultraviolet source.
10. production component according to claim 6, which is characterized in that further include air-drying device, the air-drying device is to dry
Case obtains fluorescent microsphere for air-drying the fluorescent microsphere solution.
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| Publication number | Publication date |
|---|---|
| CN109884012B (en) | 2022-04-08 |
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