CN109880781A - A kind of Novel spiral algae culture medium - Google Patents
A kind of Novel spiral algae culture medium Download PDFInfo
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- CN109880781A CN109880781A CN201910345790.XA CN201910345790A CN109880781A CN 109880781 A CN109880781 A CN 109880781A CN 201910345790 A CN201910345790 A CN 201910345790A CN 109880781 A CN109880781 A CN 109880781A
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- 241000195493 Cryptophyta Species 0.000 title claims abstract description 33
- 239000001963 growth medium Substances 0.000 title claims abstract description 26
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 67
- 239000013535 sea water Substances 0.000 claims abstract description 67
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 43
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 43
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 claims abstract description 41
- 229910001425 magnesium ion Inorganic materials 0.000 claims abstract description 41
- VTLYFUHAOXGGBS-UHFFFAOYSA-N Fe3+ Chemical compound [Fe+3] VTLYFUHAOXGGBS-UHFFFAOYSA-N 0.000 claims abstract description 34
- 229910001447 ferric ion Inorganic materials 0.000 claims abstract description 34
- 239000011780 sodium chloride Substances 0.000 claims abstract description 32
- 239000007788 liquid Substances 0.000 claims description 28
- 239000012528 membrane Substances 0.000 claims description 15
- 238000003756 stirring Methods 0.000 claims description 15
- 239000002609 medium Substances 0.000 claims description 13
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 235000016425 Arthrospira platensis Nutrition 0.000 abstract description 31
- 240000002900 Arthrospira platensis Species 0.000 abstract description 31
- 229940082787 spirulina Drugs 0.000 abstract description 31
- 238000009360 aquaculture Methods 0.000 abstract description 4
- 244000144974 aquaculture Species 0.000 abstract description 2
- 238000009313 farming Methods 0.000 description 8
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 5
- 238000005259 measurement Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 229930002868 chlorophyll a Natural products 0.000 description 3
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 239000013505 freshwater Substances 0.000 description 3
- 150000001455 metallic ions Chemical class 0.000 description 3
- 230000001228 trophic effect Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000010835 comparative analysis Methods 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000009364 mariculture Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000000967 suction filtration Methods 0.000 description 2
- 231100000033 toxigenic Toxicity 0.000 description 2
- 230000001551 toxigenic effect Effects 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 230000004300 dark adaptation Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Cultivation Of Seaweed (AREA)
Abstract
The invention discloses a kind of Novel spiral algae culture mediums, are formulated as Ca2+0.010g/L~0.015g/L, Mg2+0.015g/L~0.020g/L, Fe3+0.003g/L~0.004g/L, NaCl 1.5g/L, carbon source 0.15g/L~0.18g/L, surplus are basic seawater.The spirulina cultivated using above-mentioned Spirulina culture medium, yield is high, aquaculture cost is low.
Description
Technical field
The invention belongs to algal culture technical fields, and in particular to a kind of Novel spiral algae culture medium.
Background technique
The early stage of industry development is realized in spirulina, begins to the mariculture technology of concern spirulina in the world.
Since seawater is rich in various nutrients, sea water medium need to only add 4 kinds of elements such as carbon, nitrogen, phosphorus, iron in natural sea-water and be
Cultivation fertilizer amount is greatly reduced in the growth demand that spirulina can be met, and reduces toxigenic capacity.Research finds, spirulina pair
The high salinity of seawater and high-concentration Ca magnesium ion have good adaptability, and in high salinity environment with keeping preferable sociales
Other biological pollution is reduced in position.It is also to develop spirulina sea farming technology that freshwater resources and land cultivated land resource are increasingly in short supply
Another main cause.It is just come onto stage in recent years with the seawater spirulina industry that nature seawater is matrix culture spirulina.Benefit
Original intention with nature seawater culture spirulina is reduction toxigenic capacity, but further study show that, nature seawater culture spirulina
The yield that its active material can be improved increases the resistance of cell, and effectively avoid heavy metal ion enrichment with it is exceeded.Using
Cultivation in sea water spirulina, which has the advantage that, can develop uncultivated beach and utilize inexhaustible ocean water resource: seawater contains
There is a large amount of mineral nutrient element, fertilizer amount can be reduced；Seawater has good damping characteristics, is easier to stablize the pH of culture solution
Value；Sea water medium can be recycled for a long time.The early and middle portion stage of spirulina industry development in China, due to cultural technique
Not perfect, spirulina biology low output and quality are unstable, become the key factor of limitation spirulina industry development.Even if through
It crosses and manages with delicacy, unit area yield of biomass also can only achieve 1519g:m-2d1, and the production in commercial sizeization cultivation
Amount is less than the half of the yield.In conclusion natural sea-water cultivating spirulina contains than the nutritive component of spirulina of freshwater aquiculture
Amount is high.The cultivation of conventional helical algae is freshwater aquiculture, and mariculture technology requires more difficulty are higher to lead to sea-farming spirulina
Total output is lower, so research sea-farming spirulina promotes promotion of the trophic factors of yield to sea-farming spirulina technology
It is of great significance.
Summary of the invention
Object of the present invention is to: a kind of Novel spiral algae culture medium is provided, to solve the above problems.
The technical scheme is that
A kind of Novel spiral algae culture medium, formula are as follows: Ca2+0.010g/L~0.015g/L, Mg2+0.015g/L~
0.020g/L, Fe3+0.003g/L~0.004g/L, NaCl1.5g/L, carbon source 0.15g/L~0.18g/L, sea based on surplus
Water.
Further, Ca in the basic seawater2+For 0.4g/L, Mg2+For 1.31g/L, Fe3+It is for 0.20g/L, NaCl
32 ‰, carbon source 0.17g/L.
Further, the carbon source is CO3 2-Or HCO3 -。
Further, the pH value of the Novel spiral algae culture medium is 8.5-9.
It is of the invention another solution is that
A kind of preparation method of Novel spiral algae culture medium, comprising:
(1) seawater is first taken, the Ca in the seawater is measured2+、Mg2+、Fe3+, NaCl, carbon source concentration value；
(2) with Ca2+For 0.4g/L, Mg2+For 1.31g/L, Fe3+It is 32 ‰ for 0.20g/L, NaCl, carbon source 0.17g/L
As a reference value of basic seawater, the concentration value of the seawater is adjusted according to a reference value, obtains seawater reserve liquid, is made described
Ca in seawater reserve liquid2+、Mg2+、Fe3+, NaCl, Ca in the content of carbon source and the basic seawater2+、Mg2+、Fe3+, NaCl, carbon
The content in source is consistent；
(3) adjust the pH value of the seawater reserve liquid to 6.8-7.2,35-37 DEG C at a temperature of Fe is added3+0.003g/L
~0.004g/L stirs 3-5min in the case where centrifugal rotational speed is the revolving speed of 3000-4000r/min, crosses 0.12-0.14 μm of filter membrane,
It is 4000- in centrifugal rotational speed in 45-48 DEG C of at a temperature of addition carbon source 0.15g/L~0.18g/L that pH value, which is adjusted, to 6.3-7.0
6-8min is stirred under the revolving speed of 5000r/min, crosses 0.2-0.4 μm of filter membrane, adjusts pH value to 7.3-7.9, in 32-40 DEG C of temperature
Degree is lower to be added Ca2+0.010g/L~0.015g/L, Mg2+0.015g/L~0.020g/L is 3000-3500r/ in centrifugal rotational speed
Stir 2-3min under the revolving speed of min, cross 0.2-0.4 μm of filter membrane, 32-40 DEG C at a temperature of NaCl1.5g/L is added, from
2-3min is stirred under the revolving speed that heart revolving speed is 2000-2500r/min, obtains medium base liquid；
(4) pH value of the medium base liquid is adjusted to 8.5-9, obtains Novel spiral algae culture medium
The present invention provides a kind of Novel spiral algae culture mediums, based on seawater, change the trophic factors in seawater, i.e.,
Metallic ions Ca2+、Mg2+、Fe3+And NaCl and carbon source HCO3 -、CO3 2-, the nutritive proportion of maximum production is found, sea is reduced
Water aquaculture cost increases yield of spirulina.
Specific embodiment
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, With reference to embodiment
The present invention is described in further detail.
" one embodiment " or " embodiment " referred to herein, which refers to, may be included at least one implementation of the invention
A particular feature, structure, or characteristic." in one embodiment " that different places occur in the present specification not refers both to same
A embodiment, nor the individual or selective embodiment mutually exclusive with other embodiments.
Embodiment 1
The implementation case shows a kind of Novel spiral algae culture medium as follows:
Sea-farming spirulina under the conditions of the various concentration of the following Major Nutrient factor is selected, see Table 1 for details:
Select metallic ions Ca2+、Mg2+、Fe3+With NaCl as the primary variables factor, HCO3 -And CO3 2-To add carbon source,
Culture medium is done using same batch moon lake seawater and is grouped label, adds Ca2+Concentration range is most suitable for as one group, single is set
Five bottles are set as control, carries out that concentration tests are varied multiple times；Mg2+、Fe3+It is similarly tested with NaCl, HCO3 -And CO3 2-Addition
For the total amount of carbon source as one group, single is arranged five bottles as control, carries out that concentration tests are varied multiple times；Five groups of tests are found out respectively
Match proportional one group of conduct after most suitable range to continue to test, blunt top spirulina of the same race will be inoculated with (often after the sterilizing of all culture solutions
Bottle inoculation 4-5ml algae solution).It is cultivated in illumination box 20-30 days until adherent, cultivation temperature is set as 30 DEG C, illumination item
Part is 1000-2000Lux, and the time is set as 12Hr daytime/12Hr night.
Adopted seawater basis factor concentration is surveyed are as follows: Ca2+For 0.4g/L, Mg2+For 1.31g/L, Fe3+For 0.20g/L,
NaCl is 32 ‰, carbon source 0.17g/L, the trophic factors concentration proportioning of basis adjustment herein.
(2) measurement of upgrowth situation record and the speed of growth.
Its growth rate of results of regular determination.It is measured using optical densitometric method, timing sampling is existed with 722 type spectrophotometers
Densitometric under 560nm wavelength represents the growing state of algae with density value.[calculation formula are as follows: K=(lnN-lnN0)/t, K are
OD value, t when OD value, N0 when growth rate, N are culture the t days are culture the 1st day are culture the t days.] sampling when and survey
Timing should sufficiently shake up algae solution, and every group three bottles use its speed of growth of average value meter.
(3) yield of spirulina comparative analysis
After culture, will contain spirulina medium be placed in Suction filtration device suction filtration isolate spirulina, be put into together with filter paper
It is put into refrigerator and saves after label in storage pocket.The filter paper for being attached with spirulina is placed in 27 DEG C of baking ovens and dries 30min.So
The drying algae sample of each group is put into electronic balance afterwards and weighs and records data.(the produced algae of every bottle of culture solution will when weighing
It weighs, then every group of three bottles of produced algaes are averaged as yield of spirulina under this condition.)
(4) protein content comparative analysis
Culture obtain algae sample in, every part take the sample of equivalent be separately added into same amount of normal saline carry out grinding be made
Protein homogenate liquid, measuring it using spectrophotometer method, for the light absorption value at 280nm, then reference standard curve can in wavelength
Obtain the protein content of each part spirulina.
(5) Chlorophyll-a Content is analyzed
1. inspection apparatus connects, it is switched on after connecting well, and open PhytoWin software；2. taking out specimen cup, it is put into sample 1-
2ml (each volume should be unified), dark adaptation after closeing the lid；3. adjusting most suitable signal strength by Gain is hit；4. in Settings
It is 2 that Meas.Freq. is arranged in window, switches to Channels window, clicks SAT-Pulse, (Y at this time is Fv/ to measurement Fv/Fm
Fm)；5. it is 32 that Meas.Freq. is arranged in Settings window, it is switched to Channels window, is clicked Chl [MF32], measurement leaf is green
Plain a concentration；6. being switched to LightCurve window, clicks Start and measure photoresponse curve；7. the product of varying continue to measure；8. measuring
After the completion, it is switched to Report window, clicks File/SaveReport, storing data.Automatic measurement water sample Determination of Chlorophyll a content
(quantitative) and Chlorophyll a content.
(6) it show that data compare, finds the metallic ions Ca of most suitable sea-farming spirulina2+、Mg2+、Fe3+, and
NaCl and carbon source HCO3 -、CO3 2-Concentration compares original sea-farming yield, obtains volume increase of the scheme for original yield after improvement
Ratio.
Obtain optimal proportions conclusion formula, see Table 2 for details:
Embodiment 2
The implementation case shows a kind of preparation method of Novel spiral algae culture medium as follows:
(1) seawater is first taken, the Ca in the seawater is measured2+、Mg2+、Fe3+, NaCl, carbon source concentration value；
(2) with Ca2+For 0.4g/L, Mg2+For 1.31g/L, Fe3+It is 32 ‰ for 0.20g/L, NaCl, carbon source 0.17g/L
As a reference value of basic seawater, the concentration value of the seawater is adjusted according to a reference value, obtains seawater reserve liquid, is made described
Ca in seawater reserve liquid2+、Mg2+、Fe3+, NaCl, Ca in the content of carbon source and the basic seawater2+、Mg2+、Fe3+, NaCl, carbon
The content in source is consistent；
(3) adjust the pH value of the seawater reserve liquid to 6.8,35 DEG C at a temperature of Fe is added3+0.003g/L is being centrifuged
Stir 3min under the revolving speed that revolving speed is 3000r/min, cross 0.12 μm of filter membrane, adjustment pH value to 6.3,45 DEG C at a temperature of plus
Enter carbon source 0.15g/L, stir 6min in the case where centrifugal rotational speed is the revolving speed of 4000r/min, crosses 0.2 μm of filter membrane, adjustment pH value is extremely
7.3,32 DEG C at a temperature of Ca is added2+0.010g/L, Mg2+0.015g/L, in the case where centrifugal rotational speed is the revolving speed of 3000r/min
Stir 2min, cross 0.2 μm of filter membrane, 32 DEG C at a temperature of NaCl1.5g/L is added, be turning for 2000r/min in centrifugal rotational speed
Speed is lower to stir 2min, obtains medium base liquid；
(4) pH value of the medium base liquid is adjusted to 8.5, obtains Novel spiral algae culture medium.
Embodiment 3
The implementation case shows a kind of preparation method of Novel spiral algae culture medium as follows:
(1) seawater is first taken, the Ca in the seawater is measured2+、Mg2+、Fe3+, NaCl, carbon source concentration value；
(2) with Ca2+For 0.4g/L, Mg2+For 1.31g/L, Fe3+It is 32 ‰ for 0.20g/L, NaCl, carbon source 0.17g/L
As a reference value of basic seawater, the concentration value of the seawater is adjusted according to a reference value, obtains seawater reserve liquid, is made described
Ca in seawater reserve liquid2+、Mg2+、Fe3+, NaCl, Ca in the content of carbon source and the basic seawater2+、Mg2+、Fe3+, NaCl, carbon
The content in source is consistent；
(3) adjust the pH value of the seawater reserve liquid to 7,36 DEG C at a temperature of Fe is added3+0.0035g/L is being centrifuged
Stir 4min under the revolving speed that revolving speed is 3500r/min, cross 0.13 μm of filter membrane, adjustment pH value to 6.7,46 DEG C at a temperature of plus
Enter carbon source 0.17g/L, stir 7min in the case where centrifugal rotational speed is the revolving speed of 4500r/min, crosses 0.3 μm of filter membrane, adjustment pH value is extremely
7.7,38 DEG C at a temperature of Ca is added2+0.012g/L, Mg2+0.018g/L, in the case where centrifugal rotational speed is the revolving speed of 3200r/min
Stir 2.3min, cross 0.3 μm of filter membrane, 36 DEG C at a temperature of NaCl1.5g/L is added, be 2300r/min's in centrifugal rotational speed
2.3min is stirred under revolving speed, obtains medium base liquid；
(4) pH value of the medium base liquid is adjusted to 8.7, obtains Novel spiral algae culture medium.
Embodiment 4
The implementation case shows a kind of preparation method of Novel spiral algae culture medium as follows:
(1) seawater is first taken, the Ca in the seawater is measured2+、Mg2+、Fe3+, NaCl, carbon source concentration value；
(2) with Ca2+For 0.4g/L, Mg2+For 1.31g/L, Fe3+It is 32 ‰ for 0.20g/L, NaCl, carbon source 0.17g/L
As a reference value of basic seawater, the concentration value of the seawater is adjusted according to a reference value, obtains seawater reserve liquid, is made described
Ca in seawater reserve liquid2+、Mg2+、Fe3+, NaCl, Ca in the content of carbon source and the basic seawater2+、Mg2+、Fe3+, NaCl, carbon
The content in source is consistent；
(3) adjust the pH value of the seawater reserve liquid to 7.2,37 DEG C at a temperature of Fe is added3+0.004g/L is being centrifuged
Stir 5min under the revolving speed that revolving speed is 4000r/min, cross 0.14 μm of filter membrane, adjustment pH value to 7.0,48 DEG C at a temperature of plus
Enter carbon source 0.18g/L, stir 8min in the case where centrifugal rotational speed is the revolving speed of 5000r/min, crosses 0.4 μm of filter membrane, adjustment pH value is extremely
7.9,40 DEG C at a temperature of Ca is added2+0.015g/L, Mg2+0.020g/L, in the case where centrifugal rotational speed is the revolving speed of 3500r/min
Stir 3min, cross 0.4 μm of filter membrane, 40 DEG C at a temperature of NaCl1.5g/L is added, be turning for 2500r/min in centrifugal rotational speed
Speed is lower to stir 3min, obtains medium base liquid；
(4) pH value of the medium base liquid is adjusted to 9, obtains Novel spiral algae culture medium.
Comparative example 1
The implementation case shows a kind of preparation method of Spirulina culture medium as follows:
(1) seawater is first taken, the Ca in the seawater is measured2+、Mg2+、Fe3+, NaCl, carbon source concentration value；
(2) with Ca2+For 0.4g/L, Mg2+For 1.31g/L, Fe3+It is 32 ‰ for 0.20g/L, NaCl, carbon source 0.17g/L
As a reference value of basic seawater, the concentration value of the seawater is adjusted according to a reference value, obtains seawater reserve liquid, is made described
Ca in seawater reserve liquid2+、Mg2+、Fe3+, NaCl, Ca in the content of carbon source and the basic seawater2+、Mg2+、Fe3+, NaCl, carbon
The content in source is consistent；
(3) Fe is added3+0.003g/L~0.004g/L, Ca2+0.010g/L~0.015g/L, Mg2+0.015g/L~
0.020g/, NaCl1.5g/L, carbon source 0.15g/L~0.18g/L obtain Novel spiral algae culture medium.
Compared with prior art, the beneficial effects of the present invention are: reducing sea-farming cost, increase yield of spirulina.
It should be noted that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although referring to preferable
Embodiment describes the invention in detail, those skilled in the art should understand that, it can be to technology of the invention
Scheme is modified or replaced equivalently, and without departing from the spirit and scope of the technical solution of the present invention, should all be covered in this hair
In bright scope of the claims.
Claims (5)
1. a kind of Novel spiral algae culture medium, it is characterised in that: be formulated as Ca2+0.010g/L~0.015g/L, Mg2+0.015g/L
~0.020g/L, Fe3+0.003g/L~0.004g/L, NaCl1.5g/L, carbon source 0.15g/L~0.18g/L, sea based on surplus
Water.
2. a kind of Novel spiral algae culture medium according to claim 1, it is characterised in that: Ca in the basis seawater2+For
0.4g/L、Mg2+For 1.31g/L, Fe3+It is 32 ‰ for 0.20g/L, NaCl, carbon source 0.17g/L.
3. a kind of Novel spiral algae culture medium according to claim 1, it is characterised in that: the carbon source is CO3 2-Or
HCO3 -。
4. a kind of Novel spiral algae culture medium according to claim 1, it is characterised in that: the Novel spiral algae culture medium
PH value be 8.5-9.
5. a kind of preparation method of Novel spiral algae culture medium, it is characterised in that:
(1) seawater is first taken, the Ca in the seawater is measured2+、Mg2+、Fe3+, NaCl, carbon source concentration value；
(2) with Ca2+For 0.4g/L, Mg2+For 1.31g/L, Fe3+It is 32 ‰ for 0.20g/L, NaCl, carbon source is 0.17g/L conduct
The a reference value of basic seawater adjusts the concentration value of the seawater according to a reference value, obtains seawater reserve liquid, makes the seawater
Ca in reserve liquid2+、Mg2+、Fe3+, NaCl, Ca in the content of carbon source and the basic seawater2+、Mg2+、Fe3+, NaCl, carbon source
Content is consistent；
(3) adjust the pH value of the seawater reserve liquid to 6.8-7.2,35-37 DEG C at a temperature of Fe is added3+0.003g/L~
0.004g/L stirs 3-5min in the case where centrifugal rotational speed is the revolving speed of 3000-4000r/min, crosses 0.12-0.14 μm of filter membrane, adjust
Whole pH value is 4000- in centrifugal rotational speed in 45-48 DEG C of at a temperature of addition carbon source 0.15g/L~0.18g/L to 6.3-7.0
6-8min is stirred under the revolving speed of 5000r/min, crosses 0.2-0.4 μm of filter membrane, adjusts pH value to 7.3-7.9, in 32-40 DEG C of temperature
Degree is lower to be added Ca2+0.010g/L~0.015g/L, Mg2+0.015g/L~0.020g/L is 3000-3500r/ in centrifugal rotational speed
Stir 2-3min under the revolving speed of min, cross 0.2-0.4 μm of filter membrane, 32-40 DEG C at a temperature of NaCl1.5g/L is added, from
2-3min is stirred under the revolving speed that heart revolving speed is 2000-2500r/min, obtains medium base liquid；
(4) pH value of the medium base liquid is adjusted to 8.5-9, obtains Novel spiral algae culture medium.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101709009A (en) * | 2009-11-30 | 2010-05-19 | 北海洼地螺旋藻产业基地有限公司 | Nutrient solution for domestic culture of spirulina |
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