CN109879949A - A kind of biological agent and its preparation method and application for algae of degrading - Google Patents

A kind of biological agent and its preparation method and application for algae of degrading Download PDF

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CN109879949A
CN109879949A CN201910066010.8A CN201910066010A CN109879949A CN 109879949 A CN109879949 A CN 109879949A CN 201910066010 A CN201910066010 A CN 201910066010A CN 109879949 A CN109879949 A CN 109879949A
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陆祖宏
何春鹏
韩婷玉
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Southeast University
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    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
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    • A62D3/30—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by reacting with chemical agents
    • C—CHEMISTRY; METALLURGY
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    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

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Abstract

A kind of biological agent and its preparation method and application for algae of degrading, effective component is protein specific expressed in the epithelial cell of lancelet coecum.The present invention uses biological agent, the cost for significantly reducing algae degradation, administering.The biological agent that the present invention uses will not generate any harmful substance, avoid causing secondary pollution to environment.The biological agent that the present invention uses can effectively remove algae toxin.Algae can be degraded to nonhazardous, absorbable small molecule nutriment in a short time by the present invention, related algae catabolite by be the feed components such as dregs of beans, meat meal tankage and fish meal strong low-cost and high-quality substitute.

Description

A kind of biological agent and its preparation method and application for algae of degrading
Technical field
The invention belongs to the improvement and application field of algae (covering all seawater algaes and algae), and in particular to Yi Zhonggao The biological agent and its preparation method and application of effect degradation algae.
Background technique
Algae (such as cyanobacteria) is a kind of original and ancient unicellular organism, when temperature condition is preferable (especially in the summer Season) be easy the lake in eutrophication, reservoir, mass propagation growth in river and ocean, and with float, drift, aggregation Phenomena such as, it can do great damage to ecological environment.When such as cyanobacterial bloom, one layer of blue-green is gathered into the water surface and has bad smell Offscum etc..
With the development of the social economy, algal bloom problem has become a domestic and international main environmental problem.Algae water The harm of magnificent bring is mainly shown as: barrier air is exchanged with gas in water, it is dead after algae consumed molten because of decomposition Oxygen is solved, the dissolved oxygen concentration in water body is reduced rapidly, causes a large amount of aquatic animal anoxia asphyxias dead；Shielded from sunlight, it is unfavorable Submerged plant growth；The existence of aquatile is seriously affected, the ecological balance is destroyed, eventually leads to collapsing for entire water ecosystem It bursts；Discharge algae toxin；Water quality is destroyed, water treatment system is blocked；Landscape is influenced, peculiar smell is discharged.Therefore to Measures of Algae in Water Body Improvement becomes environmentally friendly personnel's focus of attention in recent years.
Country's algae-removing method can substantially be divided into physical method, chemical method and bioanalysis three classes at present.1) physical method mainly includes The methods of salvage, filter, settle, adsorb, but its sphere of action is small, is easy recurrence, it is at high cost.2) chemical method mainly utilizes Chlorine dioxide (ClO2), ozone (O3), potassium permanganate (KMnO4) etc. strong oxidizers oxidisability, by water algae aoxidize, reach It is not only at high cost to be also easy to cause secondary pollution to environment to the purpose for quickly killing algae.3) bioanalysis is gone out from the angle of ecology Hair, controls algae by Ecological Principles such as interbiotic nutrient competition, food chain, food webs.Microorganism is mainly used at present The methods of prevention and treatment, algophagous, water plant inhibition, although such methods have certain reference to the algae in removal water body Value, but the stable chemical structure of Microcystin can not be effectively destroyed, and Microcystin has richness on food chain The characteristic of collection, therefore it most probably passes through food chain and brings potential hazard to human body.
In terms of feed industry, although algae nutritive value is high, it is not utilized effectively so far.Mainly by Two aspect reasons cause below: 1) algae directly cannot effectively degrade due to containing cell wall for animal；2) algae contains algae poison Element, existing industrial processing method cannot effectively remove it.
Summary of the invention
The technical issues of solution: it the present invention provides biological agent of a kind of efficient degradation algae and preparation method thereof and answers With, with solve in existing algae governance process there are it is at high cost, be also easy to produce secondary pollution, can not decompose algae toxin, administer produce The problems such as object cannot efficiently use.
Technical solution: a kind of biological agent for algae of degrading, effective component are special in the epithelial cell of lancelet coecum Property expression protein.
Specifically, effective component is made of following protein: cathepsin L, cathepsin B, cathepsin D, ferritin, trypsin-like serine protease, lysozymes C, lysozymes G, carboxypeptidase Z/N, tetraspanin, legumain, saposin B, subtilisin-like protease, arylsulfatase B, endo-beta-1,4-glucanase, methionine adenosyltransferase, pancreatic lipase-like protein, plasminogen, peroxiredoxin V, VCBP4, VCBP1, VCBP3, VCBP5, Gram-negative bacteria-binding protein, alpha2-macroglobulin, chitotriosidase 1-like protein, big defensin, Proprotein convertase subtilisin/kexin type 1, Toll-interacting protein；Protein Correlated series it is as follows:
>cathepsin L
MRLLIAVVCVAMATASPLMNPQWEVFKKAYNRVYAAEEEFARRLIFEDNLKTIQMHNEEADRGLHTFRLGVNQ YADMTHKEFLENVIGGCLLDTNTSKSTADHVHEYDPTLTDLPDTVDWRDKGYVTPVKNQEQCGSCWAFSTTGSLEGQ HFKSTQKLVSLSEQNLVDCSRKRGTGLPGRLMDQGFKYIKDNGGIDTEECYPYKAKNEKCNYQASCSGATLTAKRRQ DEGRGALQQAVATVGPISVAIDAGHSSFQLYQSGVYHKFFCSETKMDHGVLAVGYGTEEGKDYWLVKNSWGASWGEK GYIKMSRNRHNNWGIATSASYPTV
>cathepsin B
MLAWVVLSVLAAVSAKEFPIHQPLTQEIIDYVNTIDTTWKAGWNFQGATVSYVKGLCGVIRDPNNHKLPLKLH ELNAQDIPDTFDSRTQWANCPTIKEVRDQGSCGSCWALAAVEAMSDRICVASKGSTMAHISAEDLNSCCKSCGNGCN GGFPEAAWEYWKRDGLVTGGPYGSHQGCQPYEIKPCEHHINGSRPACGKLEPTPRCKKSCESGYNVTFAKDKHYAKT AYSVSSKVQQIQMEIMTNGPVEAAFTVYADFPHYKSGVYQHESGAELGGHAVKMIGWGTEGSTPYWLIANSWNTDWG NMGFFKILRGQDECGIERDIVAGEPKLD
>cathepsin D
MKFLSVLFALVVFASALHRIPLTKMKTVRRQLADVGITYDQVLDKDYSGKYYNIKDAPEPLTNYLDAQYYGPI SIGTPAQNFQVVFDTGSSNLWVPSKKCKLSDIACLLHNKYDSTQSSTYMKNGTDFAIRYGSGSLTGFLSEDTVTIGG LKVQNQTFAEAVTQPGITFVAAKFDGILGMGYDTISVDGVVPPFYNMVQQKLVDKPVFSFYLNRDPSSTTRGELLLG GTDPKYYTGDFTFLDVTKPGYWQFKMDGIMINGKATDYCKGGCAAIADTGTSLIAGPTTEVQALNKQIGATPIPGGE YMVDCSQVSSLPPISFMLGGKAFELQGKDYVLQVTTMGQTVCVSGFLGIDVPAGPLWILGDVFIGPYYTLFDMGNNR VGFAPTAHVNTTRV
>ferritin
MHAMMKTLTFVLLVLQCTLGARREDNQWLISREDAGDPDNQDFARQNFHEDCEAGINKQINLEMFAGYTYRSM ASYFNRDDVALKGVADFFRHHSEEELEHAQLLEEFQNKRGGRVVYENLRKPEKDTWGSALEAMQAALTLEKNVNQRL INLHKTAGQHNDMQMQDFLDSHFMTEQVEGIKQIADYITNIKRVGTSGWASTCSTSTPSAAASRGNSVRRTREERNL HRTKSYL
>trypsin-like serine protease
MIWCLLLLALAARGDAWLFSCSASDFQCSNGNCIPGSWECDGEADCSDGSDEASCSGGGTCQQGVHFFCANGL YCIDSNWVCDGDNDCGDMSDEQNCGGGSTGTTGTTAPITSFTGDCGQPAISPQNVRVVGGVQAVQGSWPWQASLKLY GGHVCGGQIIAPNWIVTAAHCVDGQSNPSQWRVSLGSHRRTSTDSTQQDFSVTRIIMHESYDSNRINNDVALMKLSG NAQFNNYVSPICLPTQDVAAGTNCVTTGWGDTGSGASTYLMQATVPIMEWNKCNSAQYMNGAITDKMICAGYDQGGK DACQGDSGGPLVCNYSGKWTLDGIVSWGYGCAQAYKPGIYTRVTQFVSWINNKMASN
>lysozyme C
MFLAVVLVMGLVCSAHAKTYERCELARELVSRGLTSRSQAGEWICLVQHESSFRTGARGGPNWDGSYDHGLFQ INDHYWCDNGGPHNDCGVSCSNLRDNNIADDVRCAKLIYQRHGFSAWYGWQNNCQGSTSSYVSGCF
>lysozyme G
MMFLVILAIVGVATADDWQCTTSVAANGQNGQCAHVDTCPYHYFVSNKCPSYGNDVKCCYNCHLGGCSISGGS SGGTGGSGSGSYGNIMEVDTTGASSQTASQDNIWYSGVSASHQLASNDLGRLNNYKSQIFDAGNAKNMDAAVIAAII SRESRAGAALAADGTGDNGNGFGLMQVDYRYHTPAGGPYSTEHMMQGTQILIDTINCVKRNHPNWNDNMTLKGGISG YNAGCGNVQTYAGMDGGTTGEDYANDVVARAQWLKGQGF
>carboxypeptidase Z/N
MIFAILLAALPALALAEVDSSFVFEYHRYTALRSVLLAVSQDCPDITRLYSIGQSVQGRELLVLEISDNPGQH ELGEPEFKYVGNMHGNEVRGRELIILLAQYLCGEYKAGNSRIVSLVRDTRIHLMPTMNPDGFEVAANQGPDNNGWTT GRNNMQGIDLNRNFPELNSIAYSGESSGTNQDHIPIPSSYWSGTVAPETRAMITWLQSYPFVLSANMHDGDLVANYP YDTAKSGGFWGSGYAATPDDALWRDLASTYAQAHGTMATTGGGSCGFQGQGGITNGADWYSLSGGMQDFNYLHTNCY ELTLELGCDKYPRESELRMEWNNNKESLLAFMEKVHIGIKGVVTDTNGNGVADAKIKVQGNAHGVNSAADGDYWRLL RPGTYSVTATKGQASQTKSCTVGSGAATTCDFTLDAAGVLSGGTTGTGRLDISM
>tetraspanin
MEAKGGMQCIKYLLFAFNLIFWITGLAMIGVGATVKIAFGDYFDFTGNAFASAPVFLIILGVFVSIIGFLGCC GAIKENYCMVTTFAGRLSLIFILEIAAGITGYVLRNDIENVIDTAMTDFMMKWTNKTEGRLAFDGVHKKFECCGVTN YTDWFDKSPNFNPNNQPNVPGSCCLNAKTEFDVKNPDDTCGEGADTTKINTEGCLLKLEGWLAQNILIIGGVGIGIA FIQVVGIIFACCLMRSIKQEYEVV
>legumain
MLSIVMLSLFGAVLGFPADFEEAIPVAEPEPGVNWAVLIAGSTGWGNYRHQADVCHAYQILHRNGIPDERIVV MMADDLAHNIRNPTKGIIINHPDGKDVYHGVPKDYTRFDVTAKNFLRVLKGDKEGVAGIGSGKVIESGPHDNVFVYY TDHGAPGIVAMPHGGMLHADDLVTTLKEMHQENKFNKLVFYLESCESGSMFDKMLPENINIFATTAANGKESSYACY MDTKRKTYLGDLYSVNWMEDSDVEKVDKETLEQQFEKVKMLTNRSHVQEYGDVNMKNDVIGLYQSTINYPSTPLPRY KQVPHDAVPAPDVPLAILNLRLETAETEEERQTVLAELKEELNLRTTIAQTMKDIATMATDDADKAEDILVNHHRDI TARACYKAAVNYFHDKCYDLSQYEHALRHMYTLVNLCEEQVPLDRVTAAMDSVC
>saposin B
MKSVAVVLAVLLASQCLAVRPPSRRGPALVRAGGLSIGQVFKPSKVDLDLCKFCIDFSQQALNQLLNIILQVG VVGTCGDICQALADKTGSQALGVACNLLCDIEGVKEFVKIIENADLDPIYYCELLKQCTIKDDGDAKITSLDVSPKS GPQGTFMIDIEYMSKNGTGTGEMVVDIDTVDGIPLGQGFILEAQEAGSYGQKISLKAQPDPDCDPTQGPCEQWMPGV YNVNIAICNGECGSKHPHSQIYDQGMTNFTITG
>subtilisin-like protease
MKSLRVLAVLAAVLGVAMATAPMLRLDSKTAIPNEYIVVLQKNLTNYAVGKHMNSVKTLLTGTNDTKILFEHN FRWFKAYSIRTNQKMIRHLAEQPEVRYIEANQVVKAYQAQCQDQLEATWGLVRTVERDLLLDGIYHYQGGDGEGVDA YIIDTGIYTEHSEFGGRAKWGVDTVDTPSPETDENGHGTHVAGTVMSDAWGLAKKATAIAVKVLSRSGSGSTAGVIE GVEWTGQQHSGNNKKSVANMSLGGGRSDAMNEAVKAVVEAGVVMVVAAGNEGWEACNVSPASEPTAITVGCSDNEDD FCFFSNYGTCMDIIAPGQDVTSAWIGGQFADNTISGTSMSAPHIAGIVAKYMSRQSSVPSPEEVKDFLQTTSTKDKI NQIPSNSDTLNYLGFMDCGGGPDV
>arylsulfatase B
MLRVPLLLLTGALVGLVSAQDGSQNATKPHILFIVADDLGWNDVGWHNPDVKTPVLDKLAHEGVILNQSYVNY VCTPSRTAFMTGYYPYHAGSQHLVFLPQQAQGIPYNFTFLPEKLKDLGYATHMVGKWHLGFCNWKYTPTYRGFDSFY GYYNADEDYYTHVVAGGLDLRDDKEVVNTKNGQYGTYFFTDRMVDIIEKHPADTPLFLYLPFQNVHEPLEVPERFEN IYMNVQNENRRKLLGMVSALDEAVGNVTMAMKNAGLWDNTLVIFTTDNGGWIIASGNNYPLRGGKVTLWEGGTRGVA FVHGKMLQKTGYTNNEMIHAVDWFPTILAAAGGTADDAMDGVSQLDTIMSGQPSPRTEFVYNIDDVFPTKQGAIRII CTLCLRVGDYKLIEGVAGKPDGWIHPDNLTDSQLDSGDPTSGTWLFNLKDDPTEHHNLADSMPDKLKEMQAKLAEYR KSLVPAINPPPDPKSFPKNWGGAWSPGWC
>endo-beta-1,4-glucanase
MDQDYERRLLRQQNGQNCFGSPVSSPISPMAGRESLGTSPVSSPSKDKYGDRFIPSRAGANWEIGFNSIQGMY EKTSGQARKAREANSDNGKDGLAYTCLLKNELLGAGIEDLKEQTEDRRGVLSPTTPEKRNLFRYHLTAKQASPENTD HLSPYSLSPVGKKSQKLLRSPRKQTRKISKIPFKVLDAPELQDDFYLNLVDWSATNILSVGLGTCVYLWSACTSQVT RLCDLSCDGDSVTSVNWNERGNLVAVGTHKGYVQVWDAMAGKRISMLEGHSARVGALAWNADILSSGSRDRLILQRD VRTPSVVPERRLAGHRQEVCGLKWSPDHQHLASGGNDNKRIVYKKGTFVDSFRPTNMRCTALILLALLGAARAQSYY EVTGDWGDRFQANVYIPVSQAVNGWTATLKFDKAVELEIWLAEVTSTSDGNTVFELKDMGWNANVPAGTFELSFIAN TQGSAANLVGLWFNGQEVTSGGTGTGTGTGTGTGTTHRPSTVSTSHTHATAPTAAQSGTTLGVVPPVVGAYDYVDAL AKSILFYEAQRAGNLPGNNRVPWRRSCCQGDGQDVGLDLSGGWFDAGDHLKLHFPLSYTITVLSWGMIEFKRAYEAA GEMANALDSIKWVTDYLIKCHPSKFEFVAQVSTHGYSQNQILVWKYPSLVQVAKLTGHSYRVLYLAMSPDGEAIVTG AGDETLRFWNVFSKTRSNKESKSVLNLYTHIR
>methionine adenosyltransferase
MANQDTFLFTSESVGEGHPDKLCDQISDAILDAHLKKDPNAKVACEACTKTGYIMVFGEVNTTATIDYQTVVR DVIKRVGYDHSDKGFDYNTCAVIQAVEQQSPDIAQGVHEKRGAEDIGAGDQGLMFGYASDETEECMPLTCMLAHGLN RKLAELRRDGTFTWLRPDTKTQVTVEYRMSGGACVPIRVHTVVMSVQTDDVVTLDDLREQLKERVVKAVIPSRYLDD KTIYHLQPSGKFQRGGPQADAGLTGRKIIVDTYGGWGAHGGGAFSGKDFSKVDRSAGYAARWVAKSLVKAGLCRRVL VQVSYAIGISHPLSVLVNSYGTCQDGMTDARLLKIVNKNFDLRPGVIVRELGLQRPIYEKTACYGHFGRMDPDFTWE QPKKLDLNV
>pancreatic lipase-like protein
MLLSVLSILSLACAAQAAEVCYGDLGCFTNIAPFWGLNRPLSSLPDPPDVVGTKFYLYTRANPTMALRERLVA DSIATLSASHFSSSKPTRMVIHGFTGSAEHAWVQTIVDELLLKEDINVITVDWADGASIGGSYGQATANSRVVGAEV AKIVNYMSAQTGANARNFHLIGHSLGCHVAGYAGDILGNVGRITALDASEPYFDGMDAIVKLDPTDALFVDVIHSDG SPFIGTLGMGTSLPTGHVDFYPNGGEYQPGCHDNFVSSVVSTGFGLLTEGYDGAEAAAACSHLRAIDYFTESINSEC PFTAYPCESYDKFKDGFCMSCAGSTCSQMGYRARDHYGARGKLYLTTTDGTQPGGKFCAYHYKVQVTSSTRMEETAG RIYVTFQGPSGITDTMEVTDGDVMDLKPGVTYQKLVSSASNIGDVSRVGLRFERSSSIWDFFAAQYYTVGKVTITAG ETQTSYKFCGSDTPVNNGETLQLTQVGGTC
>plasminogen
MRTLLALLGLLALTAAQDCYVGRGSAYRGAVNVTSDGLECQPWSVQAPHGHAFTPANYPDAGLDGNYCRNPDN DLRPWCYTMDPNVRFGYCDAPQCDCLFNTGEFYKGSKATSISGLTCQRWDSQSPHQHDRTPQNYPEHDLRENYCRNP DGEPRTWCYTTDPNVRWEHCDVPKCDTADLGTCGVPAIQPTFSRIVGGSVATPGSWPWQVSLGYGSNGQHVCGATLI APEWVLSAAHCFAQLSTNPGSFIVKVGKHNKASTDSTEQRMQVAQIIVHPRYQQDGQNTNDIALLKLTQRVQLNDYV SQACITETEAPEGAICVATGFGNTEGTGGDNYLKQVQLPSLSNAQCRSWLGSVIKSTMVCAGYEGGGSDTCQGDSGG PLTCPRLGQWFVSGVTSFGQGCADPRKPGVYTRVGYYIDWILKYTADN
>peroxiredoxin V
MLIPVTICSLARRLPSAIGARCAIYTATAYNMPIKVGDKLPGIDLYENTPGNKVNVSELFAGKKGVLFAVPGA FTPGCSKTHLPGYVGKAGDLKAKGVQVIACVSVNDPFVMEAWGKDQKAEGKVRMLADTGAEFTKAIGLDLDATGLLG NIRSKRYSMLVEDGEVKQLNVEPDGTGLTCSLAEGLKL
>VCBP4
MAPFTPFVLCIILAGAMAQSPFRIMTVTVPRPEVTANVGSDVKLPCFYSIPAASSGLNPTINWYKGDEGIASA TKIFSLSYHGSTQIAEAYEGYELRVELESLTDPTLKLSGVRASDHGRYWCAVFDSDEHNQFGMDSKSVLLTVIDPSI QQDQGMGRPGRVELRVPAVRQVYQGEDVELTCECDDCHWTSTKSWFTVSFDDTWAATEELVATVGPCVSHQCHGGTT TVAPAFRDHFSATREALRISAAKRLDKRRYWCQVEDMTFSRGSGVDAQSVALAVESRCEGKPAGRYQHPDDCSKYYT CAEGGLQYDGISACPPGLMYDQANGYCNWATQVTCL
>VCBP1
MKFVLGLVLLAVGAHAMTIVTVSTPEPKVEASVGGSAELKCEFDIQPNSTQPPTIAWFKGNDDFRGVERIYTG HKVWGNETERREDSFGDYIGRVEVADLDKPAIKISGIKSTDFARYWCTVAEWGVRTELGVDAKSVLLTETGHSEASI DISVSGEKDVEEGGDVEMTCRCHGCTSAAIFDWFKGAFAGSEWVTTGNYTHIAAKVDVGVLGFPNPIEIDDGFGQFS VTPSNSLRLTGAQVADAGRYWCKVTSGGSVDIKATVLKVKVPEFTCAGKADGYYPDPEDCAMYYQCLYGFPQPFHRP CGYAGMVFNPEHLYCDWAFNVGPPCGSKA
>VCBP3
MQMFLLVSVCLGMAYGQSIMTVRTTHTEVEVHAGGTVELPCSYQLANDTQPPVISWLKGASPDRSTKVFKGNY NWQGEGLGFVESDSYKESFGDFLGRASVANLAAPTLRLTHVHPQDGGRYWCQVAQWSIRTEFGLDAKSVVLKVTGHT PSNNVHVSTAEVVQVDEGNDITMTCPCTDCANANVTWYTGPTFFENYETGTYQPLANKNQFGITWFSSEIAGRASFS GARNLVIRAAKITDAGRVWCELATGQGELDADRSSTILKVQLEPFTCDGKPTGLYADPTACDYYYQCIPGYPPLHRP CGYAGMVFNEEMQYCDWDINVPPPCGSKPV
>VCBP5
MSVLLVSIILLLYVGPESADAVSITTVTVPDRGGWVVMWPRPGDPTWVNRIEFRCEYSISPASATPPTITWLK GVFADADRQVIYKWSSSGEVYVHPEYAGRVSVPSRTHPTLVLTDAKFDDWGRYWCRVTNEDQSDEFGTDEESLLFWY KLGYDPPTRLSQVSLDKTPVRVATGGTARLDCTGTSGREASILWVKGPTSCTQGESCDSYETVIHKSAVWGAGNPEP ENITISPSFDGRVSLDADGSGSFTPTLTITDIRPSDSGRYWCAPDISEVYSNLGLLNRDAQSVVIIVNDPGTEPTCA GKPDGMYQHPADCAQFYTCSGGLSYGTNNCPAGLVFNQELQLCDWANNVICL
>Gram-negative bacteria-binding protein
MALLAVLLFHAALWAAGEAQGTYLGCYIDTPNDRDFPHVTTMTYTNGYTTCSQYCASLGYSYIGLQYAIECFC GHTYGKHGRAAESDCYYGCSGGTGGKCGGDYRNTVWDIGDVGGGSTGTGTSDNLELVFEDTFDTLDTTKWRPEVTAG GGGNEEFQYYMGHPDNLYVQNGNLYLKPTLASEHYGDNFMTSGYIDFWHGYNYVGCFNDGGDADLTESLLTASDMNP GKCVTHCANGGYEYFGLKNTECRCGNSFGRFGHGHGCHISCPGDSGKECGGNGVTSVYGQTYGDCTSDFPQGRACYL QGSQNAPLPPIQSARVTTVESFAFKYGKVEVWAKLPTGDWIWPAIWLLPRDNVYGGWPASGEIDIMESRGNRNLYGS WGGSIGVDDMGSTLHWGPRWPYNGFQQTTASKVEVDDQEVLLVSPPTNFWDMGNFGLPSYENPWAGGGKMTPFDREF YLIFNVAVGGTNYYFPDDATNQPYPKPWSNNNSREGAMRDFWNAKNQWYPTWNGDDVAMQIQSIKVWRFV
>alpha2-macroglobulin
MRGLLLLCGVLGSVPAQKLDPTPGPTPGTPGTFLIMAPKVVRPGRPLTLSVSILDPAAASVQVTATLLQNGRP TGTPVSGDFSRNADGTLDLVVPGALTAGSAYKLKVEGTGGLTFTEEKDLTFNSKSSSIFVQTDKGKYKPGQTVKMRA LAVDAELKTKKEPFDIDIFDPQGNEIVQWRGLQNDDGLVEKEFALSDQPLLGDWKIVAMLPGGDEVEQPFGVEEYVL PKFEVTISTNSFFARTEDTLQGTFNAKYTYGKNVEGQGKVTVKLRSVGSNFRPTDNSIEHNNIAMVEGSGSFQFTRG DIEGLYGNPDQLYYGSYQLELIVEVTEGLTGNTQEATKELDLVQTKYKLESFNTPDNFKPGLQYTAYVRTSYHDGTP MLGMDLAQPVTLSKNYGWDGPTAPPAEELTIPGSGVIKIVMDAPSTANSMTLSVMYNDISETATTHTAQKAESPSSS YIQVTTSTPEVTAGEDAQFTVKSTSPIGYFSYQVLSRGNIVVVDRVEVTPEDTLKSFTVPTNTQMAPSSRMVVYWMT DTGEICNDGLTFTVKGAFENQVSLSYDKDRAEPAEDIAVTVRADPDSLVALLTVDQGVLLLQSGNDITQDKVLAELE EYDPAEDTSNSGGPEIMWRRRKRSIWWPMPTSGDDAHSVFENAGVIVLTDAMVYQKQPDYWWQWGILPVADFDLGMM EFMPAAAAGESAGGGGGRGGLQEVKRVRSFFPETWLWTNATAGADGTAVFASTVPDTITSWVASAFALNDVSGLGVA DTPASMEAFRPFFVSLNLPYSVVNKEETAIQALVFNYMDVDMDVTVTLKGSSGFMHIVMDTNDVNSIGTEVAGDQAV VLTVPAGSSRSKSFPIIPKQFGAITVKVLAQSTLAADAVERQLLVEPEGEQKEYTKSFILDLGDSGNGDITKDLYLS VPQSGLVAGSAYARVSVMGDMMGSTISGLDSLLQMPYGCGEQTMITFAPNVYVMHYLNETNQVTPEIKSKALKFMTS GYQRELTYTHDDGSFSAFGSSDASGSTWLSAFVMKSFSQAMDYIYIDPKVLAKTIRWLIRQQQDTGVFSEPGNVIHK EMQGGLSSDVTMTSYVLVALMEYDKILATLDAPDSDKVDVTIAKNRAVAFLESRLDSETDVYTIVTMTYALTLAGSR RKDAAFQKMNSLATTEAGQMYWTKGESATSPPVSDDLMWTPPYHNPPSADVEMTAYALLTYLDRDDLVAGMPIMKWL TEQRNAYGGYSSTQDTVVGLQALGYFAAAVRAGGLDMTVTITSDTDTDFSHTFTVNDQNAIVLQRVEIPNVSGTLTA RAQGQGMGLLQLFVRYNVDVVEAVPAFNLIATVKNSDTNSVTCETCGTYQLGGSSGMAVMEIGIPSGFYVEDADLMA LVDNQAMPTLKRAERSTDNKRAVIYFDEMNASPTCVTLTSHRSMPVAKTQPSVVKVYDYYKPDVKASNIYQSETLKN TNICEVCADCTGCSGRQGRPRSAAAGLAVDTLLGVVLLLLTRLLVRQCRPTNTCEECLNVPCSELVTKLRL
>chitotriosidase 1-like protein
MLTVLALLCVMLSLGQASVIPAQRNTYRRVCYHTNWSQYRHGVGQFFPEDIDPTLCTHAIYAFAKMTNNQLQP YEWNDDSTDWSTGMYERFNTHLKPHGVKTLLGVGGWNFGSTAFSDMAATAAGRRTFVTTSIDFLRQRNFDGLDLDWE YPAARGGRPSDKQTFTLLRKELREAFDAEGARTGRAPLLLTAAMPAGEDNAMNGYEMAEVAGYLDFLNLMAYDLHGQ WETYTGLNSPLYAAGDETGDDRKLNQAFAVDMWLAGGVPPSKLNLGMGTYGRSFTTTGDNSIRAPANGGGNAGLYTR EKGYLAYYEICTMLNQGATRVFHSEHLSPYAYLGNQWVGYDDVESLTYKVEYLKSKNLGGAMVWAYDIDDFSGSSCG QGRYPLMNAIKNLLETGSAGVVLPPGPTHPPINPATQAPNPGVTQGPNPVVTQAPVVVEQGNLAAVTCDSKPDGFHP DPTDCGKYFQCWGGTMWPGHCSNGLQWNQAMLGCDWPYNVNC
>big defensin
MEKKTAYCLLFLVLLVPYTALGAVLKRAPAKKEKRAVPLAVPLVYWGASVSPAVWNWLLVTFGAAAVAAAAVT VSDNDSHSCANNRGWCRSRCFSHEYIDSWHSDVCGSYDCCRPRY
>proprotein convertase subtilisin/kexin type 1
MGRFYAWLVLAIVAFSSHGRAEDGEDQPGHFTNTWAVEIYGGPDRADLLALDHGYENLGQIGNLEDHYLFRHK DVPHRSRRGAHQHTKRLGDDERIQWVAQQVGRARSKRGPMGQQRRQSDDTRPMTFRDPYWEKQWYLHDTRTSTNLPK LDLHVLPVWRKGITGKGIVVAVLDDGIEKDHPDLVDNYDPDASYDFNDNDDDPQPRYEETNENKHGTRCAGEIAMAA NNSECGVGIAFNARIGGVRMLDGVVTDAVEASSIGFNIQHVDIYSASWGPNDDGKTVEGPEKLARAAFEKGVREGRG GKGSIFAWASGNGGSNGDNCDCDGYTSSIYTVSISSASQQGGSPWYGEKCASTLATAYSSGEYKDQKISSTDLHHEC TDSHTGTSAAAPLAAGVLALALEANPNLTWRDVQHLIVWTSEYDPLSSNPGWFQNGAGLWVNSRFGYGLLNAEAMVD MALTWKTVPEKTKCEVRIENFQPRDLGNGEEIIIELETDGCRGQNHVEALEHVQVKTTIDYSRRGDLRIVLTSPSGT STTLLDTRRQDKSQMGFQDWPFMSTHNWGEKPQGKWTLTIEDKSDHAENNGVVKDVVLILHGTPEQPAYQSGGRVYT DYNRVQGDRNVEISAAKAAPAPAADAAPARGSEERIPGSGPFGSAASVVIEEIPEKETEFLVNWQDGMNRYETDFNP VNSGPFEPSADAGSDVYMDAEDLRPWEAFKYLQRQMKSPSGQRHPAHAYNKPSSQYIRDPWVSQNALDKEANLVKYY LQLLGYES
>Toll-interacting protein
MATKVTKRGQVMLGDLPDDFLRIEATPQQLQATADMQAAQMYAQYGFMGQSSGRLSITVAQAKLAKNYGVTRM DPYCRIRVGHAVFETPTAYNGAKNPRWNKVVHAPIPQGVNSFYLEIFDERSFTMDDRVAWGHITIPESVFNGETVDD WYTLSGRQGDDKEGMINLVLSHSTVPMMQQVPQVMPQMPMMMVPQPVYYPTVQAGVPYQVPAGQMPAQQQPPAQPAI TEEDLKALQDMFPNMDAEVVRSVLEANRGNKDAAVNSLLSITAE
The preparation method of the biological agent of degradation algae, comprising the following steps: (1) obtain the coecum tissue of lancelet；(2) it extracts The full length mRNA s of coecum tissue, and it is converted into cDNA；(3) overall length is constructed using methods of homologous recombination or total gene synthesis method Functional protein group library；(4) pass through vivoexpression functional protein group product.
Specific expressed protein is in the biological agent of preparation degradation algae in the epithelial cell of lancelet coecum Using.
Preferably, above-mentioned protein and the mass ratio of algae are 1:100-1:100,000,000.
The utility model has the advantages that the present invention uses biological agent, the cost for significantly reducing algae degradation, administering.The present invention adopts Biological agent will not generate any harmful substance, avoid causing secondary pollution to environment.The biological agent that the present invention uses Algae toxin can effectively be removed.Algae can be degraded to nonhazardous in a short time by the present invention, absorbable small molecule is sought Support substance, related algae catabolite by be the feed components such as dregs of beans, meat meal tankage and fish meal strong low-cost and high-quality substitute Product.
Detailed description of the invention
Fig. 1 is that the library for the total functional protein group degraded for algae prepares embodiment.
Specific embodiment
Following is a specific embodiment of the present invention in conjunction with the accompanying drawings, technical scheme of the present invention will be further described, However, the present invention is not limited to these examples.
Embodiment 1:
1. dissecting lancelet coecum tissue；2. extracting total serum IgE；3. from Total RNAs extraction mRNA；4. such as Fig. 1, from the end 3' synthesis first Chain digests cDNA/RNA heterozygote with Rnase I, captures full-length cDNA with the end 5' Cap specific antibody and paramagnetic particle method, washs Full-length cDNA, constructs the second chain cDNA from the end 5', and obtained double-strand cDNA is carried out library construction and high-flux sequence, is utilized PYES-DEST52 carrier and Gateway methods of homologous recombination construct overall length functional protein group library, high-flux sequence；5. Recombinant vector imports saccharomyces cerevisiae (Saccharomyces cerevisiae), magnanimity expressive function protein group, abstraction function Protein group expression product；6. being mixed with Taihu Lake cyanobacteria with mass ratio 1:1,000 come algae of degrading, revolved by 24 hours 60rpm After turning, the degradation state of catabolite is detected；7. being detected using degradation effect of the LC-MS chromatography to catabolite, send out The above are molecular weight for existing catabolite 90% less than small molecules nutriments such as the oligosaccharides, oligopeptides, amino acid of 300 dalton, does not send out Existing algae toxin；8. extracting catabolite, it to be used for feed industry application.The major protein components of correlation function protein group are as follows:
>cathepsin L
MRLLIAVVCVAMATASPLMNPQWEVFKKAYNRVYAAEEEFARRLIFEDNLKTIQMHNEEADRGLHTFRLGVNQ YADMTHKEFLENVIGGCLLDTNTSKSTADHVHEYDPTLTDLPDTVDWRDKGYVTPVKNQEQCGSCWAFSTTGSLEGQ HFKSTQKLVSLSEQNLVDCSRKRGTGLPGRLMDQGFKYIKDNGGIDTEECYPYKAKNEKCNYQASCSGATLTAKRRQ DEGRGALQQAVATVGPISVAIDAGHSSFQLYQSGVYHKFFCSETKMDHGVLAVGYGTEEGKDYWLVKNSWGASWGEK GYIKMSRNRHNNWGIATSASYPTV
>cathepsin B
MLAWVVLSVLAAVSAKEFPIHQPLTQEIIDYVNTIDTTWKAGWNFQGATVSYVKGLCGVIRDPNNHKLPLKLH ELNAQDIPDTFDSRTQWANCPTIKEVRDQGSCGSCWALAAVEAMSDRICVASKGSTMAHISAEDLNSCCKSCGNGCN GGFPEAAWEYWKRDGLVTGGPYGSHQGCQPYEIKPCEHHINGSRPACGKLEPTPRCKKSCESGYNVTFAKDKHYAKT AYSVSSKVQQIQMEIMTNGPVEAAFTVYADFPHYKSGVYQHESGAELGGHAVKMIGWGTEGSTPYWLIANSWNTDWG NMGFFKILRGQDECGIERDIVAGEPKLD
>cathepsin D
MKFLSVLFALVVFASALHRIPLTKMKTVRRQLADVGITYDQVLDKDYSGKYYNIKDAPEPLTNYLDAQYYGPI SIGTPAQNFQVVFDTGSSNLWVPSKKCKLSDIACLLHNKYDSTQSSTYMKNGTDFAIRYGSGSLTGFLSEDTVTIGG LKVQNQTFAEAVTQPGITFVAAKFDGILGMGYDTISVDGVVPPFYNMVQQKLVDKPVFSFYLNRDPSSTTRGELLLG GTDPKYYTGDFTFLDVTKPGYWQFKMDGIMINGKATDYCKGGCAAIADTGTSLIAGPTTEVQALNKQIGATPIPGGE YMVDCSQVSSLPPISFMLGGKAFELQGKDYVLQVTTMGQTVCVSGFLGIDVPAGPLWILGDVFIGPYYTLFDMGNNR VGFAPTAHVNTTRV
>ferritin
MHAMMKTLTFVLLVLQCTLGARREDNQWLISREDAGDPDNQDFARQNFHEDCEAGINKQINLEMFAGYTYRSM ASYFNRDDVALKGVADFFRHHSEEELEHAQLLEEFQNKRGGRVVYENLRKPEKDTWGSALEAMQAALTLEKNVNQRL INLHKTAGQHNDMQMQDFLDSHFMTEQVEGIKQIADYITNIKRVGTSGWASTCSTSTPSAAASRGNSVRRTREERNL HRTKSYL
>trypsin-like serine protease
MIWCLLLLALAARGDAWLFSCSASDFQCSNGNCIPGSWECDGEADCSDGSDEASCSGGGTCQQGVHFFCANGL YCIDSNWVCDGDNDCGDMSDEQNCGGGSTGTTGTTAPITSFTGDCGQPAISPQNVRVVGGVQAVQGSWPWQASLKLY GGHVCGGQIIAPNWIVTAAHCVDGQSNPSQWRVSLGSHRRTSTDSTQQDFSVTRIIMHESYDSNRINNDVALMKLSG NAQFNNYVSPICLPTQDVAAGTNCVTTGWGDTGSGASTYLMQATVPIMEWNKCNSAQYMNGAITDKMICAGYDQGGK DACQGDSGGPLVCNYSGKWTLDGIVSWGYGCAQAYKPGIYTRVTQFVSWINNKMASN
>lysozyme C
MFLAVVLVMGLVCSAHAKTYERCELARELVSRGLTSRSQAGEWICLVQHESSFRTGARGGPNWDGSYDHGLFQ INDHYWCDNGGPHNDCGVSCSNLRDNNIADDVRCAKLIYQRHGFSAWYGWQNNCQGSTSSYVSGCF
>lysozyme G
MMFLVILAIVGVATADDWQCTTSVAANGQNGQCAHVDTCPYHYFVSNKCPSYGNDVKCCYNCHLGGCSISGGS SGGTGGSGSGSYGNIMEVDTTGASSQTASQDNIWYSGVSASHQLASNDLGRLNNYKSQIFDAGNAKNMDAAVIAAII SRESRAGAALAADGTGDNGNGFGLMQVDYRYHTPAGGPYSTEHMMQGTQILIDTINCVKRNHPNWNDNMTLKGGISG YNAGCGNVQTYAGMDGGTTGEDYANDVVARAQWLKGQGF
>carboxypeptidase Z/N
MIFAILLAALPALALAEVDSSFVFEYHRYTALRSVLLAVSQDCPDITRLYSIGQSVQGRELLVLEISDNPGQH ELGEPEFKYVGNMHGNEVRGRELIILLAQYLCGEYKAGNSRIVSLVRDTRIHLMPTMNPDGFEVAANQGPDNNGWTT GRNNMQGIDLNRNFPELNSIAYSGESSGTNQDHIPIPSSYWSGTVAPETRAMITWLQSYPFVLSANMHDGDLVANYP YDTAKSGGFWGSGYAATPDDALWRDLASTYAQAHGTMATTGGGSCGFQGQGGITNGADWYSLSGGMQDFNYLHTNCY ELTLELGCDKYPRESELRMEWNNNKESLLAFMEKVHIGIKGVVTDTNGNGVADAKIKVQGNAHGVNSAADGDYWRLL RPGTYSVTATKGQASQTKSCTVGSGAATTCDFTLDAAGVLSGGTTGTGRLDISM
>tetraspanin
MEAKGGMQCIKYLLFAFNLIFWITGLAMIGVGATVKIAFGDYFDFTGNAFASAPVFLIILGVFVSIIGFLGCC GAIKENYCMVTTFAGRLSLIFILEIAAGITGYVLRNDIENVIDTAMTDFMMKWTNKTEGRLAFDGVHKKFECCGVTN YTDWFDKSPNFNPNNQPNVPGSCCLNAKTEFDVKNPDDTCGEGADTTKINTEGCLLKLEGWLAQNILIIGGVGIGIA FIQVVGIIFACCLMRSIKQEYEVV
>legumain
MLSIVMLSLFGAVLGFPADFEEAIPVAEPEPGVNWAVLIAGSTGWGNYRHQADVCHAYQILHRNGIPDERIVV MMADDLAHNIRNPTKGIIINHPDGKDVYHGVPKDYTRFDVTAKNFLRVLKGDKEGVAGIGSGKVIESGPHDNVFVYY TDHGAPGIVAMPHGGMLHADDLVTTLKEMHQENKFNKLVFYLESCESGSMFDKMLPENINIFATTAANGKESSYACY MDTKRKTYLGDLYSVNWMEDSDVEKVDKETLEQQFEKVKMLTNRSHVQEYGDVNMKNDVIGLYQSTINYPSTPLPRY KQVPHDAVPAPDVPLAILNLRLETAETEEERQTVLAELKEELNLRTTIAQTMKDIATMATDDADKAEDILVNHHRDI TARACYKAAVNYFHDKCYDLSQYEHALRHMYTLVNLCEEQVPLDRVTAAMDSVC
>saposin B
MKSVAVVLAVLLASQCLAVRPPSRRGPALVRAGGLSIGQVFKPSKVDLDLCKFCIDFSQQALNQLLNIILQVG VVGTCGDICQALADKTGSQALGVACNLLCDIEGVKEFVKIIENADLDPIYYCELLKQCTIKDDGDAKITSLDVSPKS GPQGTFMIDIEYMSKNGTGTGEMVVDIDTVDGIPLGQGFILEAQEAGSYGQKISLKAQPDPDCDPTQGPCEQWMPGV YNVNIAICNGECGSKHPHSQIYDQGMTNFTITG
>subtilisin-like protease
MKSLRVLAVLAAVLGVAMATAPMLRLDSKTAIPNEYIVVLQKNLTNYAVGKHMNSVKTLLTGTNDTKILFEHN FRWFKAYSIRTNQKMIRHLAEQPEVRYIEANQVVKAYQAQCQDQLEATWGLVRTVERDLLLDGIYHYQGGDGEGVDA YIIDTGIYTEHSEFGGRAKWGVDTVDTPSPETDENGHGTHVAGTVMSDAWGLAKKATAIAVKVLSRSGSGSTAGVIE GVEWTGQQHSGNNKKSVANMSLGGGRSDAMNEAVKAVVEAGVVMVVAAGNEGWEACNVSPASEPTAITVGCSDNEDD FCFFSNYGTCMDIIAPGQDVTSAWIGGQFADNTISGTSMSAPHIAGIVAKYMSRQSSVPSPEEVKDFLQTTSTKDKI NQIPSNSDTLNYLGFMDCGGGPDV
>arylsulfatase B
MLRVPLLLLTGALVGLVSAQDGSQNATKPHILFIVADDLGWNDVGWHNPDVKTPVLDKLAHEGVILNQSYVNY VCTPSRTAFMTGYYPYHAGSQHLVFLPQQAQGIPYNFTFLPEKLKDLGYATHMVGKWHLGFCNWKYTPTYRGFDSFY GYYNADEDYYTHVVAGGLDLRDDKEVVNTKNGQYGTYFFTDRMVDIIEKHPADTPLFLYLPFQNVHEPLEVPERFEN IYMNVQNENRRKLLGMVSALDEAVGNVTMAMKNAGLWDNTLVIFTTDNGGWIIASGNNYPLRGGKVTLWEGGTRGVA FVHGKMLQKTGYTNNEMIHAVDWFPTILAAAGGTADDAMDGVSQLDTIMSGQPSPRTEFVYNIDDVFPTKQGAIRII CTLCLRVGDYKLIEGVAGKPDGWIHPDNLTDSQLDSGDPTSGTWLFNLKDDPTEHHNLADSMPDKLKEMQAKLAEYR KSLVPAINPPPDPKSFPKNWGGAWSPGWC
>endo-beta-1,4-glucanase
MDQDYERRLLRQQNGQNCFGSPVSSPISPMAGRESLGTSPVSSPSKDKYGDRFIPSRAGANWEIGFNSIQGMY EKTSGQARKAREANSDNGKDGLAYTCLLKNELLGAGIEDLKEQTEDRRGVLSPTTPEKRNLFRYHLTAKQASPENTD HLSPYSLSPVGKKSQKLLRSPRKQTRKISKIPFKVLDAPELQDDFYLNLVDWSATNILSVGLGTCVYLWSACTSQVT RLCDLSCDGDSVTSVNWNERGNLVAVGTHKGYVQVWDAMAGKRISMLEGHSARVGALAWNADILSSGSRDRLILQRD VRTPSVVPERRLAGHRQEVCGLKWSPDHQHLASGGNDNKRIVYKKGTFVDSFRPTNMRCTALILLALLGAARAQSYY EVTGDWGDRFQANVYIPVSQAVNGWTATLKFDKAVELEIWLAEVTSTSDGNTVFELKDMGWNANVPAGTFELSFIAN TQGSAANLVGLWFNGQEVTSGGTGTGTGTGTGTGTTHRPSTVSTSHTHATAPTAAQSGTTLGVVPPVVGAYDYVDAL AKSILFYEAQRAGNLPGNNRVPWRRSCCQGDGQDVGLDLSGGWFDAGDHLKLHFPLSYTITVLSWGMIEFKRAYEAA GEMANALDSIKWVTDYLIKCHPSKFEFVAQVSTHGYSQNQILVWKYPSLVQVAKLTGHSYRVLYLAMSPDGEAIVTG AGDETLRFWNVFSKTRSNKESKSVLNLYTHIR
>methionine adenosyltransferase
MANQDTFLFTSESVGEGHPDKLCDQISDAILDAHLKKDPNAKVACEACTKTGYIMVFGEVNTTATIDYQTVVR DVIKRVGYDHSDKGFDYNTCAVIQAVEQQSPDIAQGVHEKRGAEDIGAGDQGLMFGYASDETEECMPLTCMLAHGLN RKLAELRRDGTFTWLRPDTKTQVTVEYRMSGGACVPIRVHTVVMSVQTDDVVTLDDLREQLKERVVKAVIPSRYLDD KTIYHLQPSGKFQRGGPQADAGLTGRKIIVDTYGGWGAHGGGAFSGKDFSKVDRSAGYAARWVAKSLVKAGLCRRVL VQVSYAIGISHPLSVLVNSYGTCQDGMTDARLLKIVNKNFDLRPGVIVRELGLQRPIYEKTACYGHFGRMDPDFTWE QPKKLDLNV
>pancreatic lipase-like protein
MLLSVLSILSLACAAQAAEVCYGDLGCFTNIAPFWGLNRPLSSLPDPPDVVGTKFYLYTRANPTMALRERLVA DSIATLSASHFSSSKPTRMVIHGFTGSAEHAWVQTIVDELLLKEDINVITVDWADGASIGGSYGQATANSRVVGAEV AKIVNYMSAQTGANARNFHLIGHSLGCHVAGYAGDILGNVGRITALDASEPYFDGMDAIVKLDPTDALFVDVIHSDG SPFIGTLGMGTSLPTGHVDFYPNGGEYQPGCHDNFVSSVVSTGFGLLTEGYDGAEAAAACSHLRAIDYFTESINSEC PFTAYPCESYDKFKDGFCMSCAGSTCSQMGYRARDHYGARGKLYLTTTDGTQPGGKFCAYHYKVQVTSSTRMEETAG RIYVTFQGPSGITDTMEVTDGDVMDLKPGVTYQKLVSSASNIGDVSRVGLRFERSSSIWDFFAAQYYTVGKVTITAG ETQTSYKFCGSDTPVNNGETLQLTQVGGTC
>plasminogen
MRTLLALLGLLALTAAQDCYVGRGSAYRGAVNVTSDGLECQPWSVQAPHGHAFTPANYPDAGLDGNYCRNPDN DLRPWCYTMDPNVRFGYCDAPQCDCLFNTGEFYKGSKATSISGLTCQRWDSQSPHQHDRTPQNYPEHDLRENYCRNP DGEPRTWCYTTDPNVRWEHCDVPKCDTADLGTCGVPAIQPTFSRIVGGSVATPGSWPWQVSLGYGSNGQHVCGATLI APEWVLSAAHCFAQLSTNPGSFIVKVGKHNKASTDSTEQRMQVAQIIVHPRYQQDGQNTNDIALLKLTQRVQLNDYV SQACITETEAPEGAICVATGFGNTEGTGGDNYLKQVQLPSLSNAQCRSWLGSVIKSTMVCAGYEGGGSDTCQGDSGG PLTCPRLGQWFVSGVTSFGQGCADPRKPGVYTRVGYYIDWILKYTADN
>peroxiredoxin V
MLIPVTICSLARRLPSAIGARCAIYTATAYNMPIKVGDKLPGIDLYENTPGNKVNVSELFAGKKGVLFAVPGA FTPGCSKTHLPGYVGKAGDLKAKGVQVIACVSVNDPFVMEAWGKDQKAEGKVRMLADTGAEFTKAIGLDLDATGLLG NIRSKRYSMLVEDGEVKQLNVEPDGTGLTCSLAEGLKL
>VCBP4
MAPFTPFVLCIILAGAMAQSPFRIMTVTVPRPEVTANVGSDVKLPCFYSIPAASSGLNPTINWYKGDEGIASA TKIFSLSYHGSTQIAEAYEGYELRVELESLTDPTLKLSGVRASDHGRYWCAVFDSDEHNQFGMDSKSVLLTVIDPSI QQDQGMGRPGRVELRVPAVRQVYQGEDVELTCECDDCHWTSTKSWFTVSFDDTWAATEELVATVGPCVSHQCHGGTT TVAPAFRDHFSATREALRISAAKRLDKRRYWCQVEDMTFSRGSGVDAQSVALAVESRCEGKPAGRYQHPDDCSKYYT CAEGGLQYDGISACPPGLMYDQANGYCNWATQVTCL
>VCBP1
MKFVLGLVLLAVGAHAMTIVTVSTPEPKVEASVGGSAELKCEFDIQPNSTQPPTIAWFKGNDDFRGVERIYTG HKVWGNETERREDSFGDYIGRVEVADLDKPAIKISGIKSTDFARYWCTVAEWGVRTELGVDAKSVLLTETGHSEASI DISVSGEKDVEEGGDVEMTCRCHGCTSAAIFDWFKGAFAGSEWVTTGNYTHIAAKVDVGVLGFPNPIEIDDGFGQFS VTPSNSLRLTGAQVADAGRYWCKVTSGGSVDIKATVLKVKVPEFTCAGKADGYYPDPEDCAMYYQCLYGFPQPFHRP CGYAGMVFNPEHLYCDWAFNVGPPCGSKA
>VCBP3
MQMFLLVSVCLGMAYGQSIMTVRTTHTEVEVHAGGTVELPCSYQLANDTQPPVISWLKGASPDRSTKVFKGNY NWQGEGLGFVESDSYKESFGDFLGRASVANLAAPTLRLTHVHPQDGGRYWCQVAQWSIRTEFGLDAKSVVLKVTGHT PSNNVHVSTAEVVQVDEGNDITMTCPCTDCANANVTWYTGPTFFENYETGTYQPLANKNQFGITWFSSEIAGRASFS GARNLVIRAAKITDAGRVWCELATGQGELDADRSSTILKVQLEPFTCDGKPTGLYADPTACDYYYQCIPGYPPLHRP CGYAGMVFNEEMQYCDWDINVPPPCGSKPV
>VCBP5
MSVLLVSIILLLYVGPESADAVSITTVTVPDRGGWVVMWPRPGDPTWVNRIEFRCEYSISPASATPPTITWLK GVFADADRQVIYKWSSSGEVYVHPEYAGRVSVPSRTHPTLVLTDAKFDDWGRYWCRVTNEDQSDEFGTDEESLLFWY KLGYDPPTRLSQVSLDKTPVRVATGGTARLDCTGTSGREASILWVKGPTSCTQGESCDSYETVIHKSAVWGAGNPEP ENITISPSFDGRVSLDADGSGSFTPTLTITDIRPSDSGRYWCAPDISEVYSNLGLLNRDAQSVVIIVNDPGTEPTCA GKPDGMYQHPADCAQFYTCSGGLSYGTNNCPAGLVFNQELQLCDWANNVICL
>Gram-negative bacteria-binding protein
MALLAVLLFHAALWAAGEAQGTYLGCYIDTPNDRDFPHVTTMTYTNGYTTCSQYCASLGYSYIGLQYAIECFC GHTYGKHGRAAESDCYYGCSGGTGGKCGGDYRNTVWDIGDVGGGSTGTGTSDNLELVFEDTFDTLDTTKWRPEVTAG GGGNEEFQYYMGHPDNLYVQNGNLYLKPTLASEHYGDNFMTSGYIDFWHGYNYVGCFNDGGDADLTESLLTASDMNP GKCVTHCANGGYEYFGLKNTECRCGNSFGRFGHGHGCHISCPGDSGKECGGNGVTSVYGQTYGDCTSDFPQGRACYL QGSQNAPLPPIQSARVTTVESFAFKYGKVEVWAKLPTGDWIWPAIWLLPRDNVYGGWPASGEIDIMESRGNRNLYGS WGGSIGVDDMGSTLHWGPRWPYNGFQQTTASKVEVDDQEVLLVSPPTNFWDMGNFGLPSYENPWAGGGKMTPFDREF YLIFNVAVGGTNYYFPDDATNQPYPKPWSNNNSREGAMRDFWNAKNQWYPTWNGDDVAMQIQSIKVWRFV
>alpha2-macroglobulin
MRGLLLLCGVLGSVPAQKLDPTPGPTPGTPGTFLIMAPKVVRPGRPLTLSVSILDPAAASVQVTATLLQNGRP TGTPVSGDFSRNADGTLDLVVPGALTAGSAYKLKVEGTGGLTFTEEKDLTFNSKSSSIFVQTDKGKYKPGQTVKMRA LAVDAELKTKKEPFDIDIFDPQGNEIVQWRGLQNDDGLVEKEFALSDQPLLGDWKIVAMLPGGDEVEQPFGVEEYVL PKFEVTISTNSFFARTEDTLQGTFNAKYTYGKNVEGQGKVTVKLRSVGSNFRPTDNSIEHNNIAMVEGSGSFQFTRG DIEGLYGNPDQLYYGSYQLELIVEVTEGLTGNTQEATKELDLVQTKYKLESFNTPDNFKPGLQYTAYVRTSYHDGTP MLGMDLAQPVTLSKNYGWDGPTAPPAEELTIPGSGVIKIVMDAPSTANSMTLSVMYNDISETATTHTAQKAESPSSS YIQVTTSTPEVTAGEDAQFTVKSTSPIGYFSYQVLSRGNIVVVDRVEVTPEDTLKSFTVPTNTQMAPSSRMVVYWMT DTGEICNDGLTFTVKGAFENQVSLSYDKDRAEPAEDIAVTVRADPDSLVALLTVDQGVLLLQSGNDITQDKVLAELE EYDPAEDTSNSGGPEIMWRRRKRSIWWPMPTSGDDAHSVFENAGVIVLTDAMVYQKQPDYWWQWGILPVADFDLGMM EFMPAAAAGESAGGGGGRGGLQEVKRVRSFFPETWLWTNATAGADGTAVFASTVPDTITSWVASAFALNDVSGLGVA DTPASMEAFRPFFVSLNLPYSVVNKEETAIQALVFNYMDVDMDVTVTLKGSSGFMHIVMDTNDVNSIGTEVAGDQAV VLTVPAGSSRSKSFPIIPKQFGAITVKVLAQSTLAADAVERQLLVEPEGEQKEYTKSFILDLGDSGNGDITKDLYLS VPQSGLVAGSAYARVSVMGDMMGSTISGLDSLLQMPYGCGEQTMITFAPNVYVMHYLNETNQVTPEIKSKALKFMTS GYQRELTYTHDDGSFSAFGSSDASGSTWLSAFVMKSFSQAMDYIYIDPKVLAKTIRWLIRQQQDTGVFSEPGNVIHK EMQGGLSSDVTMTSYVLVALMEYDKILATLDAPDSDKVDVTIAKNRAVAFLESRLDSETDVYTIVTMTYALTLAGSR RKDAAFQKMNSLATTEAGQMYWTKGESATSPPVSDDLMWTPPYHNPPSADVEMTAYALLTYLDRDDLVAGMPIMKWL TEQRNAYGGYSSTQDTVVGLQALGYFAAAVRAGGLDMTVTITSDTDTDFSHTFTVNDQNAIVLQRVEIPNVSGTLTA RAQGQGMGLLQLFVRYNVDVVEAVPAFNLIATVKNSDTNSVTCETCGTYQLGGSSGMAVMEIGIPSGFYVEDADLMA LVDNQAMPTLKRAERSTDNKRAVIYFDEMNASPTCVTLTSHRSMPVAKTQPSVVKVYDYYKPDVKASNIYQSETLKN TNICEVCADCTGCSGRQGRPRSAAAGLAVDTLLGVVLLLLTRLLVRQCRPTNTCEECLNVPCSELVTKLRL
>chitotriosidase 1-like protein
MLTVLALLCVMLSLGQASVIPAQRNTYRRVCYHTNWSQYRHGVGQFFPEDIDPTLCTHAIYAFAKMTNNQLQP YEWNDDSTDWSTGMYERFNTHLKPHGVKTLLGVGGWNFGSTAFSDMAATAAGRRTFVTTSIDFLRQRNFDGLDLDWE YPAARGGRPSDKQTFTLLRKELREAFDAEGARTGRAPLLLTAAMPAGEDNAMNGYEMAEVAGYLDFLNLMAYDLHGQ WETYTGLNSPLYAAGDETGDDRKLNQAFAVDMWLAGGVPPSKLNLGMGTYGRSFTTTGDNSIRAPANGGGNAGLYTR EKGYLAYYEICTMLNQGATRVFHSEHLSPYAYLGNQWVGYDDVESLTYKVEYLKSKNLGGAMVWAYDIDDFSGSSCG QGRYPLMNAIKNLLETGSAGVVLPPGPTHPPINPATQAPNPGVTQGPNPVVTQAPVVVEQGNLAAVTCDSKPDGFHP DPTDCGKYFQCWGGTMWPGHCSNGLQWNQAMLGCDWPYNVNC
>big defensin
MEKKTAYCLLFLVLLVPYTALGAVLKRAPAKKEKRAVPLAVPLVYWGASVSPAVWNWLLVTFGAAAVAAAAVT VSDNDSHSCANNRGWCRSRCFSHEYIDSWHSDVCGSYDCCRPRY
>proprotein convertase subtilisin/kexin type 1
MGRFYAWLVLAIVAFSSHGRAEDGEDQPGHFTNTWAVEIYGGPDRADLLALDHGYENLGQIGNLEDHYLFRHK DVPHRSRRGAHQHTKRLGDDERIQWVAQQVGRARSKRGPMGQQRRQSDDTRPMTFRDPYWEKQWYLHDTRTSTNLPK LDLHVLPVWRKGITGKGIVVAVLDDGIEKDHPDLVDNYDPDASYDFNDNDDDPQPRYEETNENKHGTRCAGEIAMAA NNSECGVGIAFNARIGGVRMLDGVVTDAVEASSIGFNIQHVDIYSASWGPNDDGKTVEGPEKLARAAFEKGVREGRG GKGSIFAWASGNGGSNGDNCDCDGYTSSIYTVSISSASQQGGSPWYGEKCASTLATAYSSGEYKDQKISSTDLHHEC TDSHTGTSAAAPLAAGVLALALEANPNLTWRDVQHLIVWTSEYDPLSSNPGWFQNGAGLWVNSRFGYGLLNAEAMVD MALTWKTVPEKTKCEVRIENFQPRDLGNGEEIIIELETDGCRGQNHVEALEHVQVKTTIDYSRRGDLRIVLTSPSGT STTLLDTRRQDKSQMGFQDWPFMSTHNWGEKPQGKWTLTIEDKSDHAENNGVVKDVVLILHGTPEQPAYQSGGRVYT DYNRVQGDRNVEISAAKAAPAPAADAAPARGSEERIPGSGPFGSAASVVIEEIPEKETEFLVNWQDGMNRYETDFNP VNSGPFEPSADAGSDVYMDAEDLRPWEAFKYLQRQMKSPSGQRHPAHAYNKPSSQYIRDPWVSQNALDKEANLVKYY LQLLGYES
>Toll-interacting protein
MATKVTKRGQVMLGDLPDDFLRIEATPQQLQATADMQAAQMYAQYGFMGQSSGRLSITVAQAKLAKNYGVTRM DPYCRIRVGHAVFETPTAYNGAKNPRWNKVVHAPIPQGVNSFYLEIFDERSFTMDDRVAWGHITIPESVFNGETVDD WYTLSGRQGDDKEGMINLVLSHSTVPMMQQVPQVMPQMPMMMVPQPVYYPTVQAGVPYQVPAGQMPAQQQPPAQPAI TEEDLKALQDMFPNMDAEVVRSVLEANRGNKDAAVNSLLSITAE
The above is only a preferred embodiment of the present invention, it is noted that those skilled in the art are come It says, several improvement can also be made without departing from the principle of the present invention, these improvement also should be regarded as protection of the invention Range.
Sequence table
<110>Southeast China University
<120>a kind of biological agent and its preparation method and application for algae of degrading
<160> 28
<170> SIPOSequenceListing 1.0
<210> 1
<211> 328
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 1
Met Arg Leu Leu Ile Ala Val Val Cys Val Ala Met Ala Thr Ala Ser
1 5 10 15
Pro Leu Met Asn Pro Gln Trp Glu Val Phe Lys Lys Ala Tyr Asn Arg
20 25 30
Val Tyr Ala Ala Glu Glu Glu Phe Ala Arg Arg Leu Ile Phe Glu Asp
35 40 45
Asn Leu Lys Thr Ile Gln Met His Asn Glu Glu Ala Asp Arg Gly Leu
50 55 60
His Thr Phe Arg Leu Gly Val Asn Gln Tyr Ala Asp Met Thr His Lys
65 70 75 80
Glu Phe Leu Glu Asn Val Ile Gly Gly Cys Leu Leu Asp Thr Asn Thr
85 90 95
Ser Lys Ser Thr Ala Asp His Val His Glu Tyr Asp Pro Thr Leu Thr
100 105 110
Asp Leu Pro Asp Thr Val Asp Trp Arg Asp Lys Gly Tyr Val Thr Pro
115 120 125
Val Lys Asn Gln Glu Gln Cys Gly Ser Cys Trp Ala Phe Ser Thr Thr
130 135 140
Gly Ser Leu Glu Gly Gln His Phe Lys Ser Thr Gln Lys Leu Val Ser
145 150 155 160
Leu Ser Glu Gln Asn Leu Val Asp Cys Ser Arg Lys Arg Gly Thr Gly
165 170 175
Leu Pro Gly Arg Leu Met Asp Gln Gly Phe Lys Tyr Ile Lys Asp Asn
180 185 190
Gly Gly Ile Asp Thr Glu Glu Cys Tyr Pro Tyr Lys Ala Lys Asn Glu
195 200 205
Lys Cys Asn Tyr Gln Ala Ser Cys Ser Gly Ala Thr Leu Thr Ala Lys
210 215 220
Arg Arg Gln Asp Glu Gly Arg Gly Ala Leu Gln Gln Ala Val Ala Thr
225 230 235 240
Val Gly Pro Ile Ser Val Ala Ile Asp Ala Gly His Ser Ser Phe Gln
245 250 255
Leu Tyr Gln Ser Gly Val Tyr His Lys Phe Phe Cys Ser Glu Thr Lys
260 265 270
Met Asp His Gly Val Leu Ala Val Gly Tyr Gly Thr Glu Glu Gly Lys
275 280 285
Asp Tyr Trp Leu Val Lys Asn Ser Trp Gly Ala Ser Trp Gly Glu Lys
290 295 300
Gly Tyr Ile Lys Met Ser Arg Asn Arg His Asn Asn Trp Gly Ile Ala
305 310 315 320
Thr Ser Ala Ser Tyr Pro Thr Val
325
<210> 2
<211> 332
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 2
Met Leu Ala Trp Val Val Leu Ser Val Leu Ala Ala Val Ser Ala Lys
1 5 10 15
Glu Phe Pro Ile His Gln Pro Leu Thr Gln Glu Ile Ile Asp Tyr Val
20 25 30
Asn Thr Ile Asp Thr Thr Trp Lys Ala Gly Trp Asn Phe Gln Gly Ala
35 40 45
Thr Val Ser Tyr Val Lys Gly Leu Cys Gly Val Ile Arg Asp Pro Asn
50 55 60
Asn His Lys Leu Pro Leu Lys Leu His Glu Leu Asn Ala Gln Asp Ile
65 70 75 80
Pro Asp Thr Phe Asp Ser Arg Thr Gln Trp Ala Asn Cys Pro Thr Ile
85 90 95
Lys Glu Val Arg Asp Gln Gly Ser Cys Gly Ser Cys Trp Ala Leu Ala
100 105 110
Ala Val Glu Ala Met Ser Asp Arg Ile Cys Val Ala Ser Lys Gly Ser
115 120 125
Thr Met Ala His Ile Ser Ala Glu Asp Leu Asn Ser Cys Cys Lys Ser
130 135 140
Cys Gly Asn Gly Cys Asn Gly Gly Phe Pro Glu Ala Ala Trp Glu Tyr
145 150 155 160
Trp Lys Arg Asp Gly Leu Val Thr Gly Gly Pro Tyr Gly Ser His Gln
165 170 175
Gly Cys Gln Pro Tyr Glu Ile Lys Pro Cys Glu His His Ile Asn Gly
180 185 190
Ser Arg Pro Ala Cys Gly Lys Leu Glu Pro Thr Pro Arg Cys Lys Lys
195 200 205
Ser Cys Glu Ser Gly Tyr Asn Val Thr Phe Ala Lys Asp Lys His Tyr
210 215 220
Ala Lys Thr Ala Tyr Ser Val Ser Ser Lys Val Gln Gln Ile Gln Met
225 230 235 240
Glu Ile Met Thr Asn Gly Pro Val Glu Ala Ala Phe Thr Val Tyr Ala
245 250 255
Asp Phe Pro His Tyr Lys Ser Gly Val Tyr Gln His Glu Ser Gly Ala
260 265 270
Glu Leu Gly Gly His Ala Val Lys Met Ile Gly Trp Gly Thr Glu Gly
275 280 285
Ser Thr Pro Tyr Trp Leu Ile Ala Asn Ser Trp Asn Thr Asp Trp Gly
290 295 300
Asn Met Gly Phe Phe Lys Ile Leu Arg Gly Gln Asp Glu Cys Gly Ile
305 310 315 320
Glu Arg Asp Ile Val Ala Gly Glu Pro Lys Leu Asp
325 330
<210> 3
<211> 395
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 3
Met Lys Phe Leu Ser Val Leu Phe Ala Leu Val Val Phe Ala Ser Ala
1 5 10 15
Leu His Arg Ile Pro Leu Thr Lys Met Lys Thr Val Arg Arg Gln Leu
20 25 30
Ala Asp Val Gly Ile Thr Tyr Asp Gln Val Leu Asp Lys Asp Tyr Ser
35 40 45
Gly Lys Tyr Tyr Asn Ile Lys Asp Ala Pro Glu Pro Leu Thr Asn Tyr
50 55 60
Leu Asp Ala Gln Tyr Tyr Gly Pro Ile Ser Ile Gly Thr Pro Ala Gln
65 70 75 80
Asn Phe Gln Val Val Phe Asp Thr Gly Ser Ser Asn Leu Trp Val Pro
85 90 95
Ser Lys Lys Cys Lys Leu Ser Asp Ile Ala Cys Leu Leu His Asn Lys
100 105 110
Tyr Asp Ser Thr Gln Ser Ser Thr Tyr Met Lys Asn Gly Thr Asp Phe
115 120 125
Ala Ile Arg Tyr Gly Ser Gly Ser Leu Thr Gly Phe Leu Ser Glu Asp
130 135 140
Thr Val Thr Ile Gly Gly Leu Lys Val Gln Asn Gln Thr Phe Ala Glu
145 150 155 160
Ala Val Thr Gln Pro Gly Ile Thr Phe Val Ala Ala Lys Phe Asp Gly
165 170 175
Ile Leu Gly Met Gly Tyr Asp Thr Ile Ser Val Asp Gly Val Val Pro
180 185 190
Pro Phe Tyr Asn Met Val Gln Gln Lys Leu Val Asp Lys Pro Val Phe
195 200 205
Ser Phe Tyr Leu Asn Arg Asp Pro Ser Ser Thr Thr Arg Gly Glu Leu
210 215 220
Leu Leu Gly Gly Thr Asp Pro Lys Tyr Tyr Thr Gly Asp Phe Thr Phe
225 230 235 240
Leu Asp Val Thr Lys Pro Gly Tyr Trp Gln Phe Lys Met Asp Gly Ile
245 250 255
Met Ile Asn Gly Lys Ala Thr Asp Tyr Cys Lys Gly Gly Cys Ala Ala
260 265 270
Ile Ala Asp Thr Gly Thr Ser Leu Ile Ala Gly Pro Thr Thr Glu Val
275 280 285
Gln Ala Leu Asn Lys Gln Ile Gly Ala Thr Pro Ile Pro Gly Gly Glu
290 295 300
Tyr Met Val Asp Cys Ser Gln Val Ser Ser Leu Pro Pro Ile Ser Phe
305 310 315 320
Met Leu Gly Gly Lys Ala Phe Glu Leu Gln Gly Lys Asp Tyr Val Leu
325 330 335
Gln Val Thr Thr Met Gly Gln Thr Val Cys Val Ser Gly Phe Leu Gly
340 345 350
Ile Asp Val Pro Ala Gly Pro Leu Trp Ile Leu Gly Asp Val Phe Ile
355 360 365
Gly Pro Tyr Tyr Thr Leu Phe Asp Met Gly Asn Asn Arg Val Gly Phe
370 375 380
Ala Pro Thr Ala His Val Asn Thr Thr Arg Val
385 390 395
<210> 4
<211> 234
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 4
Met His Ala Met Met Lys Thr Leu Thr Phe Val Leu Leu Val Leu Gln
1 5 10 15
Cys Thr Leu Gly Ala Arg Arg Glu Asp Asn Gln Trp Leu Ile Ser Arg
20 25 30
Glu Asp Ala Gly Asp Pro Asp Asn Gln Asp Phe Ala Arg Gln Asn Phe
35 40 45
His Glu Asp Cys Glu Ala Gly Ile Asn Lys Gln Ile Asn Leu Glu Met
50 55 60
Phe Ala Gly Tyr Thr Tyr Arg Ser Met Ala Ser Tyr Phe Asn Arg Asp
65 70 75 80
Asp Val Ala Leu Lys Gly Val Ala Asp Phe Phe Arg His His Ser Glu
85 90 95
Glu Glu Leu Glu His Ala Gln Leu Leu Glu Glu Phe Gln Asn Lys Arg
100 105 110
Gly Gly Arg Val Val Tyr Glu Asn Leu Arg Lys Pro Glu Lys Asp Thr
115 120 125
Trp Gly Ser Ala Leu Glu Ala Met Gln Ala Ala Leu Thr Leu Glu Lys
130 135 140
Asn Val Asn Gln Arg Leu Ile Asn Leu His Lys Thr Ala Gly Gln His
145 150 155 160
Asn Asp Met Gln Met Gln Asp Phe Leu Asp Ser His Phe Met Thr Glu
165 170 175
Gln Val Glu Gly Ile Lys Gln Ile Ala Asp Tyr Ile Thr Asn Ile Lys
180 185 190
Arg Val Gly Thr Ser Gly Trp Ala Ser Thr Cys Ser Thr Ser Thr Pro
195 200 205
Ser Ala Ala Ala Ser Arg Gly Asn Ser Val Arg Arg Thr Arg Glu Glu
210 215 220
Arg Asn Leu His Arg Thr Lys Ser Tyr Leu
225 230
<210> 5
<211> 361
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 5
Met Ile Trp Cys Leu Leu Leu Leu Ala Leu Ala Ala Arg Gly Asp Ala
1 5 10 15
Trp Leu Phe Ser Cys Ser Ala Ser Asp Phe Gln Cys Ser Asn Gly Asn
20 25 30
Cys Ile Pro Gly Ser Trp Glu Cys Asp Gly Glu Ala Asp Cys Ser Asp
35 40 45
Gly Ser Asp Glu Ala Ser Cys Ser Gly Gly Gly Thr Cys Gln Gln Gly
50 55 60
Val His Phe Phe Cys Ala Asn Gly Leu Tyr Cys Ile Asp Ser Asn Trp
65 70 75 80
Val Cys Asp Gly Asp Asn Asp Cys Gly Asp Met Ser Asp Glu Gln Asn
85 90 95
Cys Gly Gly Gly Ser Thr Gly Thr Thr Gly Thr Thr Ala Pro Ile Thr
100 105 110
Ser Phe Thr Gly Asp Cys Gly Gln Pro Ala Ile Ser Pro Gln Asn Val
115 120 125
Arg Val Val Gly Gly Val Gln Ala Val Gln Gly Ser Trp Pro Trp Gln
130 135 140
Ala Ser Leu Lys Leu Tyr Gly Gly His Val Cys Gly Gly Gln Ile Ile
145 150 155 160
Ala Pro Asn Trp Ile Val Thr Ala Ala His Cys Val Asp Gly Gln Ser
165 170 175
Asn Pro Ser Gln Trp Arg Val Ser Leu Gly Ser His Arg Arg Thr Ser
180 185 190
Thr Asp Ser Thr Gln Gln Asp Phe Ser Val Thr Arg Ile Ile Met His
195 200 205
Glu Ser Tyr Asp Ser Asn Arg Ile Asn Asn Asp Val Ala Leu Met Lys
210 215 220
Leu Ser Gly Asn Ala Gln Phe Asn Asn Tyr Val Ser Pro Ile Cys Leu
225 230 235 240
Pro Thr Gln Asp Val Ala Ala Gly Thr Asn Cys Val Thr Thr Gly Trp
245 250 255
Gly Asp Thr Gly Ser Gly Ala Ser Thr Tyr Leu Met Gln Ala Thr Val
260 265 270
Pro Ile Met Glu Trp Asn Lys Cys Asn Ser Ala Gln Tyr Met Asn Gly
275 280 285
Ala Ile Thr Asp Lys Met Ile Cys Ala Gly Tyr Asp Gln Gly Gly Lys
290 295 300
Asp Ala Cys Gln Gly Asp Ser Gly Gly Pro Leu Val Cys Asn Tyr Ser
305 310 315 320
Gly Lys Trp Thr Leu Asp Gly Ile Val Ser Trp Gly Tyr Gly Cys Ala
325 330 335
Gln Ala Tyr Lys Pro Gly Ile Tyr Thr Arg Val Thr Gln Phe Val Ser
340 345 350
Trp Ile Asn Asn Lys Met Ala Ser Asn
355 360
<210> 6
<211> 139
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 6
Met Phe Leu Ala Val Val Leu Val Met Gly Leu Val Cys Ser Ala His
1 5 10 15
Ala Lys Thr Tyr Glu Arg Cys Glu Leu Ala Arg Glu Leu Val Ser Arg
20 25 30
Gly Leu Thr Ser Arg Ser Gln Ala Gly Glu Trp Ile Cys Leu Val Gln
35 40 45
His Glu Ser Ser Phe Arg Thr Gly Ala Arg Gly Gly Pro Asn Trp Asp
50 55 60
Gly Ser Tyr Asp His Gly Leu Phe Gln Ile Asn Asp His Tyr Trp Cys
65 70 75 80
Asp Asn Gly Gly Pro His Asn Asp Cys Gly Val Ser Cys Ser Asn Leu
85 90 95
Arg Asp Asn Asn Ile Ala Asp Asp Val Arg Cys Ala Lys Leu Ile Tyr
100 105 110
Gln Arg His Gly Phe Ser Ala Trp Tyr Gly Trp Gln Asn Asn Cys Gln
115 120 125
Gly Ser Thr Ser Ser Tyr Val Ser Gly Cys Phe
130 135
<210> 7
<211> 266
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 7
Met Met Phe Leu Val Ile Leu Ala Ile Val Gly Val Ala Thr Ala Asp
1 5 10 15
Asp Trp Gln Cys Thr Thr Ser Val Ala Ala Asn Gly Gln Asn Gly Gln
20 25 30
Cys Ala His Val Asp Thr Cys Pro Tyr His Tyr Phe Val Ser Asn Lys
35 40 45
Cys Pro Ser Tyr Gly Asn Asp Val Lys Cys Cys Tyr Asn Cys His Leu
50 55 60
Gly Gly Cys Ser Ile Ser Gly Gly Ser Ser Gly Gly Thr Gly Gly Ser
65 70 75 80
Gly Ser Gly Ser Tyr Gly Asn Ile Met Glu Val Asp Thr Thr Gly Ala
85 90 95
Ser Ser Gln Thr Ala Ser Gln Asp Asn Ile Trp Tyr Ser Gly Val Ser
100 105 110
Ala Ser His Gln Leu Ala Ser Asn Asp Leu Gly Arg Leu Asn Asn Tyr
115 120 125
Lys Ser Gln Ile Phe Asp Ala Gly Asn Ala Lys Asn Met Asp Ala Ala
130 135 140
Val Ile Ala Ala Ile Ile Ser Arg Glu Ser Arg Ala Gly Ala Ala Leu
145 150 155 160
Ala Ala Asp Gly Thr Gly Asp Asn Gly Asn Gly Phe Gly Leu Met Gln
165 170 175
Val Asp Tyr Arg Tyr His Thr Pro Ala Gly Gly Pro Tyr Ser Thr Glu
180 185 190
His Met Met Gln Gly Thr Gln Ile Leu Ile Asp Thr Ile Asn Cys Val
195 200 205
Lys Arg Asn His Pro Asn Trp Asn Asp Asn Met Thr Leu Lys Gly Gly
210 215 220
Ile Ser Gly Tyr Asn Ala Gly Cys Gly Asn Val Gln Thr Tyr Ala Gly
225 230 235 240
Met Asp Gly Gly Thr Thr Gly Glu Asp Tyr Ala Asn Asp Val Val Ala
245 250 255
Arg Ala Gln Trp Leu Lys Gly Gln Gly Phe
260 265
<210> 8
<211> 435
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 8
Met Ile Phe Ala Ile Leu Leu Ala Ala Leu Pro Ala Leu Ala Leu Ala
1 5 10 15
Glu Val Asp Ser Ser Phe Val Phe Glu Tyr His Arg Tyr Thr Ala Leu
20 25 30
Arg Ser Val Leu Leu Ala Val Ser Gln Asp Cys Pro Asp Ile Thr Arg
35 40 45
Leu Tyr Ser Ile Gly Gln Ser Val Gln Gly Arg Glu Leu Leu Val Leu
50 55 60
Glu Ile Ser Asp Asn Pro Gly Gln His Glu Leu Gly Glu Pro Glu Phe
65 70 75 80
Lys Tyr Val Gly Asn Met His Gly Asn Glu Val Arg Gly Arg Glu Leu
85 90 95
Ile Ile Leu Leu Ala Gln Tyr Leu Cys Gly Glu Tyr Lys Ala Gly Asn
100 105 110
Ser Arg Ile Val Ser Leu Val Arg Asp Thr Arg Ile His Leu Met Pro
115 120 125
Thr Met Asn Pro Asp Gly Phe Glu Val Ala Ala Asn Gln Gly Pro Asp
130 135 140
Asn Asn Gly Trp Thr Thr Gly Arg Asn Asn Met Gln Gly Ile Asp Leu
145 150 155 160
Asn Arg Asn Phe Pro Glu Leu Asn Ser Ile Ala Tyr Ser Gly Glu Ser
165 170 175
Ser Gly Thr Asn Gln Asp His Ile Pro Ile Pro Ser Ser Tyr Trp Ser
180 185 190
Gly Thr Val Ala Pro Glu Thr Arg Ala Met Ile Thr Trp Leu Gln Ser
195 200 205
Tyr Pro Phe Val Leu Ser Ala Asn Met His Asp Gly Asp Leu Val Ala
210 215 220
Asn Tyr Pro Tyr Asp Thr Ala Lys Ser Gly Gly Phe Trp Gly Ser Gly
225 230 235 240
Tyr Ala Ala Thr Pro Asp Asp Ala Leu Trp Arg Asp Leu Ala Ser Thr
245 250 255
Tyr Ala Gln Ala His Gly Thr Met Ala Thr Thr Gly Gly Gly Ser Cys
260 265 270
Gly Phe Gln Gly Gln Gly Gly Ile Thr Asn Gly Ala Asp Trp Tyr Ser
275 280 285
Leu Ser Gly Gly Met Gln Asp Phe Asn Tyr Leu His Thr Asn Cys Tyr
290 295 300
Glu Leu Thr Leu Glu Leu Gly Cys Asp Lys Tyr Pro Arg Glu Ser Glu
305 310 315 320
Leu Arg Met Glu Trp Asn Asn Asn Lys Glu Ser Leu Leu Ala Phe Met
325 330 335
Glu Lys Val His Ile Gly Ile Lys Gly Val Val Thr Asp Thr Asn Gly
340 345 350
Asn Gly Val Ala Asp Ala Lys Ile Lys Val Gln Gly Asn Ala His Gly
355 360 365
Val Asn Ser Ala Ala Asp Gly Asp Tyr Trp Arg Leu Leu Arg Pro Gly
370 375 380
Thr Tyr Ser Val Thr Ala Thr Lys Gly Gln Ala Ser Gln Thr Lys Ser
385 390 395 400
Cys Thr Val Gly Ser Gly Ala Ala Thr Thr Cys Asp Phe Thr Leu Asp
405 410 415
Ala Ala Gly Val Leu Ser Gly Gly Thr Thr Gly Thr Gly Arg Leu Asp
420 425 430
Ile Ser Met
435
<210> 9
<211> 251
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 9
Met Glu Ala Lys Gly Gly Met Gln Cys Ile Lys Tyr Leu Leu Phe Ala
1 5 10 15
Phe Asn Leu Ile Phe Trp Ile Thr Gly Leu Ala Met Ile Gly Val Gly
20 25 30
Ala Thr Val Lys Ile Ala Phe Gly Asp Tyr Phe Asp Phe Thr Gly Asn
35 40 45
Ala Phe Ala Ser Ala Pro Val Phe Leu Ile Ile Leu Gly Val Phe Val
50 55 60
Ser Ile Ile Gly Phe Leu Gly Cys Cys Gly Ala Ile Lys Glu Asn Tyr
65 70 75 80
Cys Met Val Thr Thr Phe Ala Gly Arg Leu Ser Leu Ile Phe Ile Leu
85 90 95
Glu Ile Ala Ala Gly Ile Thr Gly Tyr Val Leu Arg Asn Asp Ile Glu
100 105 110
Asn Val Ile Asp Thr Ala Met Thr Asp Phe Met Met Lys Trp Thr Asn
115 120 125
Lys Thr Glu Gly Arg Leu Ala Phe Asp Gly Val His Lys Lys Phe Glu
130 135 140
Cys Cys Gly Val Thr Asn Tyr Thr Asp Trp Phe Asp Lys Ser Pro Asn
145 150 155 160
Phe Asn Pro Asn Asn Gln Pro Asn Val Pro Gly Ser Cys Cys Leu Asn
165 170 175
Ala Lys Thr Glu Phe Asp Val Lys Asn Pro Asp Asp Thr Cys Gly Glu
180 185 190
Gly Ala Asp Thr Thr Lys Ile Asn Thr Glu Gly Cys Leu Leu Lys Leu
195 200 205
Glu Gly Trp Leu Ala Gln Asn Ile Leu Ile Ile Gly Gly Val Gly Ile
210 215 220
Gly Ile Ala Phe Ile Gln Val Val Gly Ile Ile Phe Ala Cys Cys Leu
225 230 235 240
Met Arg Ser Ile Lys Gln Glu Tyr Glu Val Val
245 250
<210> 10
<211> 435
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 10
Met Leu Ser Ile Val Met Leu Ser Leu Phe Gly Ala Val Leu Gly Phe
1 5 10 15
Pro Ala Asp Phe Glu Glu Ala Ile Pro Val Ala Glu Pro Glu Pro Gly
20 25 30
Val Asn Trp Ala Val Leu Ile Ala Gly Ser Thr Gly Trp Gly Asn Tyr
35 40 45
Arg His Gln Ala Asp Val Cys His Ala Tyr Gln Ile Leu His Arg Asn
50 55 60
Gly Ile Pro Asp Glu Arg Ile Val Val Met Met Ala Asp Asp Leu Ala
65 70 75 80
His Asn Ile Arg Asn Pro Thr Lys Gly Ile Ile Ile Asn His Pro Asp
85 90 95
Gly Lys Asp Val Tyr His Gly Val Pro Lys Asp Tyr Thr Arg Phe Asp
100 105 110
Val Thr Ala Lys Asn Phe Leu Arg Val Leu Lys Gly Asp Lys Glu Gly
115 120 125
Val Ala Gly Ile Gly Ser Gly Lys Val Ile Glu Ser Gly Pro His Asp
130 135 140
Asn Val Phe Val Tyr Tyr Thr Asp His Gly Ala Pro Gly Ile Val Ala
145 150 155 160
Met Pro His Gly Gly Met Leu His Ala Asp Asp Leu Val Thr Thr Leu
165 170 175
Lys Glu Met His Gln Glu Asn Lys Phe Asn Lys Leu Val Phe Tyr Leu
180 185 190
Glu Ser Cys Glu Ser Gly Ser Met Phe Asp Lys Met Leu Pro Glu Asn
195 200 205
Ile Asn Ile Phe Ala Thr Thr Ala Ala Asn Gly Lys Glu Ser Ser Tyr
210 215 220
Ala Cys Tyr Met Asp Thr Lys Arg Lys Thr Tyr Leu Gly Asp Leu Tyr
225 230 235 240
Ser Val Asn Trp Met Glu Asp Ser Asp Val Glu Lys Val Asp Lys Glu
245 250 255
Thr Leu Glu Gln Gln Phe Glu Lys Val Lys Met Leu Thr Asn Arg Ser
260 265 270
His Val Gln Glu Tyr Gly Asp Val Asn Met Lys Asn Asp Val Ile Gly
275 280 285
Leu Tyr Gln Ser Thr Ile Asn Tyr Pro Ser Thr Pro Leu Pro Arg Tyr
290 295 300
Lys Gln Val Pro His Asp Ala Val Pro Ala Pro Asp Val Pro Leu Ala
305 310 315 320
Ile Leu Asn Leu Arg Leu Glu Thr Ala Glu Thr Glu Glu Glu Arg Gln
325 330 335
Thr Val Leu Ala Glu Leu Lys Glu Glu Leu Asn Leu Arg Thr Thr Ile
340 345 350
Ala Gln Thr Met Lys Asp Ile Ala Thr Met Ala Thr Asp Asp Ala Asp
355 360 365
Lys Ala Glu Asp Ile Leu Val Asn His His Arg Asp Ile Thr Ala Arg
370 375 380
Ala Cys Tyr Lys Ala Ala Val Asn Tyr Phe His Asp Lys Cys Tyr Asp
385 390 395 400
Leu Ser Gln Tyr Glu His Ala Leu Arg His Met Tyr Thr Leu Val Asn
405 410 415
Leu Cys Glu Glu Gln Val Pro Leu Asp Arg Val Thr Ala Ala Met Asp
420 425 430
Ser Val Cys
435
<210> 11
<211> 260
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 11
Met Lys Ser Val Ala Val Val Leu Ala Val Leu Leu Ala Ser Gln Cys
1 5 10 15
Leu Ala Val Arg Pro Pro Ser Arg Arg Gly Pro Ala Leu Val Arg Ala
20 25 30
Gly Gly Leu Ser Ile Gly Gln Val Phe Lys Pro Ser Lys Val Asp Leu
35 40 45
Asp Leu Cys Lys Phe Cys Ile Asp Phe Ser Gln Gln Ala Leu Asn Gln
50 55 60
Leu Leu Asn Ile Ile Leu Gln Val Gly Val Val Gly Thr Cys Gly Asp
65 70 75 80
Ile Cys Gln Ala Leu Ala Asp Lys Thr Gly Ser Gln Ala Leu Gly Val
85 90 95
Ala Cys Asn Leu Leu Cys Asp Ile Glu Gly Val Lys Glu Phe Val Lys
100 105 110
Ile Ile Glu Asn Ala Asp Leu Asp Pro Ile Tyr Tyr Cys Glu Leu Leu
115 120 125
Lys Gln Cys Thr Ile Lys Asp Asp Gly Asp Ala Lys Ile Thr Ser Leu
130 135 140
Asp Val Ser Pro Lys Ser Gly Pro Gln Gly Thr Phe Met Ile Asp Ile
145 150 155 160
Glu Tyr Met Ser Lys Asn Gly Thr Gly Thr Gly Glu Met Val Val Asp
165 170 175
Ile Asp Thr Val Asp Gly Ile Pro Leu Gly Gln Gly Phe Ile Leu Glu
180 185 190
Ala Gln Glu Ala Gly Ser Tyr Gly Gln Lys Ile Ser Leu Lys Ala Gln
195 200 205
Pro Asp Pro Asp Cys Asp Pro Thr Gln Gly Pro Cys Glu Gln Trp Met
210 215 220
Pro Gly Val Tyr Asn Val Asn Ile Ala Ile Cys Asn Gly Glu Cys Gly
225 230 235 240
Ser Lys His Pro His Ser Gln Ile Tyr Asp Gln Gly Met Thr Asn Phe
245 250 255
Thr Ile Thr Gly
260
<210> 12
<211> 405
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 12
Met Lys Ser Leu Arg Val Leu Ala Val Leu Ala Ala Val Leu Gly Val
1 5 10 15
Ala Met Ala Thr Ala Pro Met Leu Arg Leu Asp Ser Lys Thr Ala Ile
20 25 30
Pro Asn Glu Tyr Ile Val Val Leu Gln Lys Asn Leu Thr Asn Tyr Ala
35 40 45
Val Gly Lys His Met Asn Ser Val Lys Thr Leu Leu Thr Gly Thr Asn
50 55 60
Asp Thr Lys Ile Leu Phe Glu His Asn Phe Arg Trp Phe Lys Ala Tyr
65 70 75 80
Ser Ile Arg Thr Asn Gln Lys Met Ile Arg His Leu Ala Glu Gln Pro
85 90 95
Glu Val Arg Tyr Ile Glu Ala Asn Gln Val Val Lys Ala Tyr Gln Ala
100 105 110
Gln Cys Gln Asp Gln Leu Glu Ala Thr Trp Gly Leu Val Arg Thr Val
115 120 125
Glu Arg Asp Leu Leu Leu Asp Gly Ile Tyr His Tyr Gln Gly Gly Asp
130 135 140
Gly Glu Gly Val Asp Ala Tyr Ile Ile Asp Thr Gly Ile Tyr Thr Glu
145 150 155 160
His Ser Glu Phe Gly Gly Arg Ala Lys Trp Gly Val Asp Thr Val Asp
165 170 175
Thr Pro Ser Pro Glu Thr Asp Glu Asn Gly His Gly Thr His Val Ala
180 185 190
Gly Thr Val Met Ser Asp Ala Trp Gly Leu Ala Lys Lys Ala Thr Ala
195 200 205
Ile Ala Val Lys Val Leu Ser Arg Ser Gly Ser Gly Ser Thr Ala Gly
210 215 220
Val Ile Glu Gly Val Glu Trp Thr Gly Gln Gln His Ser Gly Asn Asn
225 230 235 240
Lys Lys Ser Val Ala Asn Met Ser Leu Gly Gly Gly Arg Ser Asp Ala
245 250 255
Met Asn Glu Ala Val Lys Ala Val Val Glu Ala Gly Val Val Met Val
260 265 270
Val Ala Ala Gly Asn Glu Gly Trp Glu Ala Cys Asn Val Ser Pro Ala
275 280 285
Ser Glu Pro Thr Ala Ile Thr Val Gly Cys Ser Asp Asn Glu Asp Asp
290 295 300
Phe Cys Phe Phe Ser Asn Tyr Gly Thr Cys Met Asp Ile Ile Ala Pro
305 310 315 320
Gly Gln Asp Val Thr Ser Ala Trp Ile Gly Gly Gln Phe Ala Asp Asn
325 330 335
Thr Ile Ser Gly Thr Ser Met Ser Ala Pro His Ile Ala Gly Ile Val
340 345 350
Ala Lys Tyr Met Ser Arg Gln Ser Ser Val Pro Ser Pro Glu Glu Val
355 360 365
Lys Asp Phe Leu Gln Thr Thr Ser Thr Lys Asp Lys Ile Asn Gln Ile
370 375 380
Pro Ser Asn Ser Asp Thr Leu Asn Tyr Leu Gly Phe Met Asp Cys Gly
385 390 395 400
Gly Gly Pro Asp Val
405
<210> 13
<211> 487
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 13
Met Leu Arg Val Pro Leu Leu Leu Leu Thr Gly Ala Leu Val Gly Leu
1 5 10 15
Val Ser Ala Gln Asp Gly Ser Gln Asn Ala Thr Lys Pro His Ile Leu
20 25 30
Phe Ile Val Ala Asp Asp Leu Gly Trp Asn Asp Val Gly Trp His Asn
35 40 45
Pro Asp Val Lys Thr Pro Val Leu Asp Lys Leu Ala His Glu Gly Val
50 55 60
Ile Leu Asn Gln Ser Tyr Val Asn Tyr Val Cys Thr Pro Ser Arg Thr
65 70 75 80
Ala Phe Met Thr Gly Tyr Tyr Pro Tyr His Ala Gly Ser Gln His Leu
85 90 95
Val Phe Leu Pro Gln Gln Ala Gln Gly Ile Pro Tyr Asn Phe Thr Phe
100 105 110
Leu Pro Glu Lys Leu Lys Asp Leu Gly Tyr Ala Thr His Met Val Gly
115 120 125
Lys Trp His Leu Gly Phe Cys Asn Trp Lys Tyr Thr Pro Thr Tyr Arg
130 135 140
Gly Phe Asp Ser Phe Tyr Gly Tyr Tyr Asn Ala Asp Glu Asp Tyr Tyr
145 150 155 160
Thr His Val Val Ala Gly Gly Leu Asp Leu Arg Asp Asp Lys Glu Val
165 170 175
Val Asn Thr Lys Asn Gly Gln Tyr Gly Thr Tyr Phe Phe Thr Asp Arg
180 185 190
Met Val Asp Ile Ile Glu Lys His Pro Ala Asp Thr Pro Leu Phe Leu
195 200 205
Tyr Leu Pro Phe Gln Asn Val His Glu Pro Leu Glu Val Pro Glu Arg
210 215 220
Phe Glu Asn Ile Tyr Met Asn Val Gln Asn Glu Asn Arg Arg Lys Leu
225 230 235 240
Leu Gly Met Val Ser Ala Leu Asp Glu Ala Val Gly Asn Val Thr Met
245 250 255
Ala Met Lys Asn Ala Gly Leu Trp Asp Asn Thr Leu Val Ile Phe Thr
260 265 270
Thr Asp Asn Gly Gly Trp Ile Ile Ala Ser Gly Asn Asn Tyr Pro Leu
275 280 285
Arg Gly Gly Lys Val Thr Leu Trp Glu Gly Gly Thr Arg Gly Val Ala
290 295 300
Phe Val His Gly Lys Met Leu Gln Lys Thr Gly Tyr Thr Asn Asn Glu
305 310 315 320
Met Ile His Ala Val Asp Trp Phe Pro Thr Ile Leu Ala Ala Ala Gly
325 330 335
Gly Thr Ala Asp Asp Ala Met Asp Gly Val Ser Gln Leu Asp Thr Ile
340 345 350
Met Ser Gly Gln Pro Ser Pro Arg Thr Glu Phe Val Tyr Asn Ile Asp
355 360 365
Asp Val Phe Pro Thr Lys Gln Gly Ala Ile Arg Ile Ile Cys Thr Leu
370 375 380
Cys Leu Arg Val Gly Asp Tyr Lys Leu Ile Glu Gly Val Ala Gly Lys
385 390 395 400
Pro Asp Gly Trp Ile His Pro Asp Asn Leu Thr Asp Ser Gln Leu Asp
405 410 415
Ser Gly Asp Pro Thr Ser Gly Thr Trp Leu Phe Asn Leu Lys Asp Asp
420 425 430
Pro Thr Glu His His Asn Leu Ala Asp Ser Met Pro Asp Lys Leu Lys
435 440 445
Glu Met Gln Ala Lys Leu Ala Glu Tyr Arg Lys Ser Leu Val Pro Ala
450 455 460
Ile Asn Pro Pro Pro Asp Pro Lys Ser Phe Pro Lys Asn Trp Gly Gly
465 470 475 480
Ala Trp Ser Pro Gly Trp Cys
485
<210> 14
<211> 721
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 14
Met Asp Gln Asp Tyr Glu Arg Arg Leu Leu Arg Gln Gln Asn Gly Gln
1 5 10 15
Asn Cys Phe Gly Ser Pro Val Ser Ser Pro Ile Ser Pro Met Ala Gly
20 25 30
Arg Glu Ser Leu Gly Thr Ser Pro Val Ser Ser Pro Ser Lys Asp Lys
35 40 45
Tyr Gly Asp Arg Phe Ile Pro Ser Arg Ala Gly Ala Asn Trp Glu Ile
50 55 60
Gly Phe Asn Ser Ile Gln Gly Met Tyr Glu Lys Thr Ser Gly Gln Ala
65 70 75 80
Arg Lys Ala Arg Glu Ala Asn Ser Asp Asn Gly Lys Asp Gly Leu Ala
85 90 95
Tyr Thr Cys Leu Leu Lys Asn Glu Leu Leu Gly Ala Gly Ile Glu Asp
100 105 110
Leu Lys Glu Gln Thr Glu Asp Arg Arg Gly Val Leu Ser Pro Thr Thr
115 120 125
Pro Glu Lys Arg Asn Leu Phe Arg Tyr His Leu Thr Ala Lys Gln Ala
130 135 140
Ser Pro Glu Asn Thr Asp His Leu Ser Pro Tyr Ser Leu Ser Pro Val
145 150 155 160
Gly Lys Lys Ser Gln Lys Leu Leu Arg Ser Pro Arg Lys Gln Thr Arg
165 170 175
Lys Ile Ser Lys Ile Pro Phe Lys Val Leu Asp Ala Pro Glu Leu Gln
180 185 190
Asp Asp Phe Tyr Leu Asn Leu Val Asp Trp Ser Ala Thr Asn Ile Leu
195 200 205
Ser Val Gly Leu Gly Thr Cys Val Tyr Leu Trp Ser Ala Cys Thr Ser
210 215 220
Gln Val Thr Arg Leu Cys Asp Leu Ser Cys Asp Gly Asp Ser Val Thr
225 230 235 240
Ser Val Asn Trp Asn Glu Arg Gly Asn Leu Val Ala Val Gly Thr His
245 250 255
Lys Gly Tyr Val Gln Val Trp Asp Ala Met Ala Gly Lys Arg Ile Ser
260 265 270
Met Leu Glu Gly His Ser Ala Arg Val Gly Ala Leu Ala Trp Asn Ala
275 280 285
Asp Ile Leu Ser Ser Gly Ser Arg Asp Arg Leu Ile Leu Gln Arg Asp
290 295 300
Val Arg Thr Pro Ser Val Val Pro Glu Arg Arg Leu Ala Gly His Arg
305 310 315 320
Gln Glu Val Cys Gly Leu Lys Trp Ser Pro Asp His Gln His Leu Ala
325 330 335
Ser Gly Gly Asn Asp Asn Lys Arg Ile Val Tyr Lys Lys Gly Thr Phe
340 345 350
Val Asp Ser Phe Arg Pro Thr Asn Met Arg Cys Thr Ala Leu Ile Leu
355 360 365
Leu Ala Leu Leu Gly Ala Ala Arg Ala Gln Ser Tyr Tyr Glu Val Thr
370 375 380
Gly Asp Trp Gly Asp Arg Phe Gln Ala Asn Val Tyr Ile Pro Val Ser
385 390 395 400
Gln Ala Val Asn Gly Trp Thr Ala Thr Leu Lys Phe Asp Lys Ala Val
405 410 415
Glu Leu Glu Ile Trp Leu Ala Glu Val Thr Ser Thr Ser Asp Gly Asn
420 425 430
Thr Val Phe Glu Leu Lys Asp Met Gly Trp Asn Ala Asn Val Pro Ala
435 440 445
Gly Thr Phe Glu Leu Ser Phe Ile Ala Asn Thr Gln Gly Ser Ala Ala
450 455 460
Asn Leu Val Gly Leu Trp Phe Asn Gly Gln Glu Val Thr Ser Gly Gly
465 470 475 480
Thr Gly Thr Gly Thr Gly Thr Gly Thr Gly Thr Gly Thr Thr His Arg
485 490 495
Pro Ser Thr Val Ser Thr Ser His Thr His Ala Thr Ala Pro Thr Ala
500 505 510
Ala Gln Ser Gly Thr Thr Leu Gly Val Val Pro Pro Val Val Gly Ala
515 520 525
Tyr Asp Tyr Val Asp Ala Leu Ala Lys Ser Ile Leu Phe Tyr Glu Ala
530 535 540
Gln Arg Ala Gly Asn Leu Pro Gly Asn Asn Arg Val Pro Trp Arg Arg
545 550 555 560
Ser Cys Cys Gln Gly Asp Gly Gln Asp Val Gly Leu Asp Leu Ser Gly
565 570 575
Gly Trp Phe Asp Ala Gly Asp His Leu Lys Leu His Phe Pro Leu Ser
580 585 590
Tyr Thr Ile Thr Val Leu Ser Trp Gly Met Ile Glu Phe Lys Arg Ala
595 600 605
Tyr Glu Ala Ala Gly Glu Met Ala Asn Ala Leu Asp Ser Ile Lys Trp
610 615 620
Val Thr Asp Tyr Leu Ile Lys Cys His Pro Ser Lys Phe Glu Phe Val
625 630 635 640
Ala Gln Val Ser Thr His Gly Tyr Ser Gln Asn Gln Ile Leu Val Trp
645 650 655
Lys Tyr Pro Ser Leu Val Gln Val Ala Lys Leu Thr Gly His Ser Tyr
660 665 670
Arg Val Leu Tyr Leu Ala Met Ser Pro Asp Gly Glu Ala Ile Val Thr
675 680 685
Gly Ala Gly Asp Glu Thr Leu Arg Phe Trp Asn Val Phe Ser Lys Thr
690 695 700
Arg Ser Asn Lys Glu Ser Lys Ser Val Leu Asn Leu Tyr Thr His Ile
705 710 715 720
Arg
<210> 15
<211> 390
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 15
Met Ala Asn Gln Asp Thr Phe Leu Phe Thr Ser Glu Ser Val Gly Glu
1 5 10 15
Gly His Pro Asp Lys Leu Cys Asp Gln Ile Ser Asp Ala Ile Leu Asp
20 25 30
Ala His Leu Lys Lys Asp Pro Asn Ala Lys Val Ala Cys Glu Ala Cys
35 40 45
Thr Lys Thr Gly Tyr Ile Met Val Phe Gly Glu Val Asn Thr Thr Ala
50 55 60
Thr Ile Asp Tyr Gln Thr Val Val Arg Asp Val Ile Lys Arg Val Gly
65 70 75 80
Tyr Asp His Ser Asp Lys Gly Phe Asp Tyr Asn Thr Cys Ala Val Ile
85 90 95
Gln Ala Val Glu Gln Gln Ser Pro Asp Ile Ala Gln Gly Val His Glu
100 105 110
Lys Arg Gly Ala Glu Asp Ile Gly Ala Gly Asp Gln Gly Leu Met Phe
115 120 125
Gly Tyr Ala Ser Asp Glu Thr Glu Glu Cys Met Pro Leu Thr Cys Met
130 135 140
Leu Ala His Gly Leu Asn Arg Lys Leu Ala Glu Leu Arg Arg Asp Gly
145 150 155 160
Thr Phe Thr Trp Leu Arg Pro Asp Thr Lys Thr Gln Val Thr Val Glu
165 170 175
Tyr Arg Met Ser Gly Gly Ala Cys Val Pro Ile Arg Val His Thr Val
180 185 190
Val Met Ser Val Gln Thr Asp Asp Val Val Thr Leu Asp Asp Leu Arg
195 200 205
Glu Gln Leu Lys Glu Arg Val Val Lys Ala Val Ile Pro Ser Arg Tyr
210 215 220
Leu Asp Asp Lys Thr Ile Tyr His Leu Gln Pro Ser Gly Lys Phe Gln
225 230 235 240
Arg Gly Gly Pro Gln Ala Asp Ala Gly Leu Thr Gly Arg Lys Ile Ile
245 250 255
Val Asp Thr Tyr Gly Gly Trp Gly Ala His Gly Gly Gly Ala Phe Ser
260 265 270
Gly Lys Asp Phe Ser Lys Val Asp Arg Ser Ala Gly Tyr Ala Ala Arg
275 280 285
Trp Val Ala Lys Ser Leu Val Lys Ala Gly Leu Cys Arg Arg Val Leu
290 295 300
Val Gln Val Ser Tyr Ala Ile Gly Ile Ser His Pro Leu Ser Val Leu
305 310 315 320
Val Asn Ser Tyr Gly Thr Cys Gln Asp Gly Met Thr Asp Ala Arg Leu
325 330 335
Leu Lys Ile Val Asn Lys Asn Phe Asp Leu Arg Pro Gly Val Ile Val
340 345 350
Arg Glu Leu Gly Leu Gln Arg Pro Ile Tyr Glu Lys Thr Ala Cys Tyr
355 360 365
Gly His Phe Gly Arg Met Asp Pro Asp Phe Thr Trp Glu Gln Pro Lys
370 375 380
Lys Leu Asp Leu Asn Val
385 390
<210> 16
<211> 488
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 16
Met Leu Leu Ser Val Leu Ser Ile Leu Ser Leu Ala Cys Ala Ala Gln
1 5 10 15
Ala Ala Glu Val Cys Tyr Gly Asp Leu Gly Cys Phe Thr Asn Ile Ala
20 25 30
Pro Phe Trp Gly Leu Asn Arg Pro Leu Ser Ser Leu Pro Asp Pro Pro
35 40 45
Asp Val Val Gly Thr Lys Phe Tyr Leu Tyr Thr Arg Ala Asn Pro Thr
50 55 60
Met Ala Leu Arg Glu Arg Leu Val Ala Asp Ser Ile Ala Thr Leu Ser
65 70 75 80
Ala Ser His Phe Ser Ser Ser Lys Pro Thr Arg Met Val Ile His Gly
85 90 95
Phe Thr Gly Ser Ala Glu His Ala Trp Val Gln Thr Ile Val Asp Glu
100 105 110
Leu Leu Leu Lys Glu Asp Ile Asn Val Ile Thr Val Asp Trp Ala Asp
115 120 125
Gly Ala Ser Ile Gly Gly Ser Tyr Gly Gln Ala Thr Ala Asn Ser Arg
130 135 140
Val Val Gly Ala Glu Val Ala Lys Ile Val Asn Tyr Met Ser Ala Gln
145 150 155 160
Thr Gly Ala Asn Ala Arg Asn Phe His Leu Ile Gly His Ser Leu Gly
165 170 175
Cys His Val Ala Gly Tyr Ala Gly Asp Ile Leu Gly Asn Val Gly Arg
180 185 190
Ile Thr Ala Leu Asp Ala Ser Glu Pro Tyr Phe Asp Gly Met Asp Ala
195 200 205
Ile Val Lys Leu Asp Pro Thr Asp Ala Leu Phe Val Asp Val Ile His
210 215 220
Ser Asp Gly Ser Pro Phe Ile Gly Thr Leu Gly Met Gly Thr Ser Leu
225 230 235 240
Pro Thr Gly His Val Asp Phe Tyr Pro Asn Gly Gly Glu Tyr Gln Pro
245 250 255
Gly Cys His Asp Asn Phe Val Ser Ser Val Val Ser Thr Gly Phe Gly
260 265 270
Leu Leu Thr Glu Gly Tyr Asp Gly Ala Glu Ala Ala Ala Ala Cys Ser
275 280 285
His Leu Arg Ala Ile Asp Tyr Phe Thr Glu Ser Ile Asn Ser Glu Cys
290 295 300
Pro Phe Thr Ala Tyr Pro Cys Glu Ser Tyr Asp Lys Phe Lys Asp Gly
305 310 315 320
Phe Cys Met Ser Cys Ala Gly Ser Thr Cys Ser Gln Met Gly Tyr Arg
325 330 335
Ala Arg Asp His Tyr Gly Ala Arg Gly Lys Leu Tyr Leu Thr Thr Thr
340 345 350
Asp Gly Thr Gln Pro Gly Gly Lys Phe Cys Ala Tyr His Tyr Lys Val
355 360 365
Gln Val Thr Ser Ser Thr Arg Met Glu Glu Thr Ala Gly Arg Ile Tyr
370 375 380
Val Thr Phe Gln Gly Pro Ser Gly Ile Thr Asp Thr Met Glu Val Thr
385 390 395 400
Asp Gly Asp Val Met Asp Leu Lys Pro Gly Val Thr Tyr Gln Lys Leu
405 410 415
Val Ser Ser Ala Ser Asn Ile Gly Asp Val Ser Arg Val Gly Leu Arg
420 425 430
Phe Glu Arg Ser Ser Ser Ile Trp Asp Phe Phe Ala Ala Gln Tyr Tyr
435 440 445
Thr Val Gly Lys Val Thr Ile Thr Ala Gly Glu Thr Gln Thr Ser Tyr
450 455 460
Lys Phe Cys Gly Ser Asp Thr Pro Val Asn Asn Gly Glu Thr Leu Gln
465 470 475 480
Leu Thr Gln Val Gly Gly Thr Cys
485
<210> 17
<211> 429
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 17
Met Arg Thr Leu Leu Ala Leu Leu Gly Leu Leu Ala Leu Thr Ala Ala
1 5 10 15
Gln Asp Cys Tyr Val Gly Arg Gly Ser Ala Tyr Arg Gly Ala Val Asn
20 25 30
Val Thr Ser Asp Gly Leu Glu Cys Gln Pro Trp Ser Val Gln Ala Pro
35 40 45
His Gly His Ala Phe Thr Pro Ala Asn Tyr Pro Asp Ala Gly Leu Asp
50 55 60
Gly Asn Tyr Cys Arg Asn Pro Asp Asn Asp Leu Arg Pro Trp Cys Tyr
65 70 75 80
Thr Met Asp Pro Asn Val Arg Phe Gly Tyr Cys Asp Ala Pro Gln Cys
85 90 95
Asp Cys Leu Phe Asn Thr Gly Glu Phe Tyr Lys Gly Ser Lys Ala Thr
100 105 110
Ser Ile Ser Gly Leu Thr Cys Gln Arg Trp Asp Ser Gln Ser Pro His
115 120 125
Gln His Asp Arg Thr Pro Gln Asn Tyr Pro Glu His Asp Leu Arg Glu
130 135 140
Asn Tyr Cys Arg Asn Pro Asp Gly Glu Pro Arg Thr Trp Cys Tyr Thr
145 150 155 160
Thr Asp Pro Asn Val Arg Trp Glu His Cys Asp Val Pro Lys Cys Asp
165 170 175
Thr Ala Asp Leu Gly Thr Cys Gly Val Pro Ala Ile Gln Pro Thr Phe
180 185 190
Ser Arg Ile Val Gly Gly Ser Val Ala Thr Pro Gly Ser Trp Pro Trp
195 200 205
Gln Val Ser Leu Gly Tyr Gly Ser Asn Gly Gln His Val Cys Gly Ala
210 215 220
Thr Leu Ile Ala Pro Glu Trp Val Leu Ser Ala Ala His Cys Phe Ala
225 230 235 240
Gln Leu Ser Thr Asn Pro Gly Ser Phe Ile Val Lys Val Gly Lys His
245 250 255
Asn Lys Ala Ser Thr Asp Ser Thr Glu Gln Arg Met Gln Val Ala Gln
260 265 270
Ile Ile Val His Pro Arg Tyr Gln Gln Asp Gly Gln Asn Thr Asn Asp
275 280 285
Ile Ala Leu Leu Lys Leu Thr Gln Arg Val Gln Leu Asn Asp Tyr Val
290 295 300
Ser Gln Ala Cys Ile Thr Glu Thr Glu Ala Pro Glu Gly Ala Ile Cys
305 310 315 320
Val Ala Thr Gly Phe Gly Asn Thr Glu Gly Thr Gly Gly Asp Asn Tyr
325 330 335
Leu Lys Gln Val Gln Leu Pro Ser Leu Ser Asn Ala Gln Cys Arg Ser
340 345 350
Trp Leu Gly Ser Val Ile Lys Ser Thr Met Val Cys Ala Gly Tyr Glu
355 360 365
Gly Gly Gly Ser Asp Thr Cys Gln Gly Asp Ser Gly Gly Pro Leu Thr
370 375 380
Cys Pro Arg Leu Gly Gln Trp Phe Val Ser Gly Val Thr Ser Phe Gly
385 390 395 400
Gln Gly Cys Ala Asp Pro Arg Lys Pro Gly Val Tyr Thr Arg Val Gly
405 410 415
Tyr Tyr Ile Asp Trp Ile Leu Lys Tyr Thr Ala Asp Asn
420 425
<210> 18
<211> 188
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 18
Met Leu Ile Pro Val Thr Ile Cys Ser Leu Ala Arg Arg Leu Pro Ser
1 5 10 15
Ala Ile Gly Ala Arg Cys Ala Ile Tyr Thr Ala Thr Ala Tyr Asn Met
20 25 30
Pro Ile Lys Val Gly Asp Lys Leu Pro Gly Ile Asp Leu Tyr Glu Asn
35 40 45
Thr Pro Gly Asn Lys Val Asn Val Ser Glu Leu Phe Ala Gly Lys Lys
50 55 60
Gly Val Leu Phe Ala Val Pro Gly Ala Phe Thr Pro Gly Cys Ser Lys
65 70 75 80
Thr His Leu Pro Gly Tyr Val Gly Lys Ala Gly Asp Leu Lys Ala Lys
85 90 95
Gly Val Gln Val Ile Ala Cys Val Ser Val Asn Asp Pro Phe Val Met
100 105 110
Glu Ala Trp Gly Lys Asp Gln Lys Ala Glu Gly Lys Val Arg Met Leu
115 120 125
Ala Asp Thr Gly Ala Glu Phe Thr Lys Ala Ile Gly Leu Asp Leu Asp
130 135 140
Ala Thr Gly Leu Leu Gly Asn Ile Arg Ser Lys Arg Tyr Ser Met Leu
145 150 155 160
Val Glu Asp Gly Glu Val Lys Gln Leu Asn Val Glu Pro Asp Gly Thr
165 170 175
Gly Leu Thr Cys Ser Leu Ala Glu Gly Leu Lys Leu
180 185
<210> 19
<211> 340
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 19
Met Ala Pro Phe Thr Pro Phe Val Leu Cys Ile Ile Leu Ala Gly Ala
1 5 10 15
Met Ala Gln Ser Pro Phe Arg Ile Met Thr Val Thr Val Pro Arg Pro
20 25 30
Glu Val Thr Ala Asn Val Gly Ser Asp Val Lys Leu Pro Cys Phe Tyr
35 40 45
Ser Ile Pro Ala Ala Ser Ser Gly Leu Asn Pro Thr Ile Asn Trp Tyr
50 55 60
Lys Gly Asp Glu Gly Ile Ala Ser Ala Thr Lys Ile Phe Ser Leu Ser
65 70 75 80
Tyr His Gly Ser Thr Gln Ile Ala Glu Ala Tyr Glu Gly Tyr Glu Leu
85 90 95
Arg Val Glu Leu Glu Ser Leu Thr Asp Pro Thr Leu Lys Leu Ser Gly
100 105 110
Val Arg Ala Ser Asp His Gly Arg Tyr Trp Cys Ala Val Phe Asp Ser
115 120 125
Asp Glu His Asn Gln Phe Gly Met Asp Ser Lys Ser Val Leu Leu Thr
130 135 140
Val Ile Asp Pro Ser Ile Gln Gln Asp Gln Gly Met Gly Arg Pro Gly
145 150 155 160
Arg Val Glu Leu Arg Val Pro Ala Val Arg Gln Val Tyr Gln Gly Glu
165 170 175
Asp Val Glu Leu Thr Cys Glu Cys Asp Asp Cys His Trp Thr Ser Thr
180 185 190
Lys Ser Trp Phe Thr Val Ser Phe Asp Asp Thr Trp Ala Ala Thr Glu
195 200 205
Glu Leu Val Ala Thr Val Gly Pro Cys Val Ser His Gln Cys His Gly
210 215 220
Gly Thr Thr Thr Val Ala Pro Ala Phe Arg Asp His Phe Ser Ala Thr
225 230 235 240
Arg Glu Ala Leu Arg Ile Ser Ala Ala Lys Arg Leu Asp Lys Arg Arg
245 250 255
Tyr Trp Cys Gln Val Glu Asp Met Thr Phe Ser Arg Gly Ser Gly Val
260 265 270
Asp Ala Gln Ser Val Ala Leu Ala Val Glu Ser Arg Cys Glu Gly Lys
275 280 285
Pro Ala Gly Arg Tyr Gln His Pro Asp Asp Cys Ser Lys Tyr Tyr Thr
290 295 300
Cys Ala Glu Gly Gly Leu Gln Tyr Asp Gly Ile Ser Ala Cys Pro Pro
305 310 315 320
Gly Leu Met Tyr Asp Gln Ala Asn Gly Tyr Cys Asn Trp Ala Thr Gln
325 330 335
Val Thr Cys Leu
340
<210> 20
<211> 333
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 20
Met Lys Phe Val Leu Gly Leu Val Leu Leu Ala Val Gly Ala His Ala
1 5 10 15
Met Thr Ile Val Thr Val Ser Thr Pro Glu Pro Lys Val Glu Ala Ser
20 25 30
Val Gly Gly Ser Ala Glu Leu Lys Cys Glu Phe Asp Ile Gln Pro Asn
35 40 45
Ser Thr Gln Pro Pro Thr Ile Ala Trp Phe Lys Gly Asn Asp Asp Phe
50 55 60
Arg Gly Val Glu Arg Ile Tyr Thr Gly His Lys Val Trp Gly Asn Glu
65 70 75 80
Thr Glu Arg Arg Glu Asp Ser Phe Gly Asp Tyr Ile Gly Arg Val Glu
85 90 95
Val Ala Asp Leu Asp Lys Pro Ala Ile Lys Ile Ser Gly Ile Lys Ser
100 105 110
Thr Asp Phe Ala Arg Tyr Trp Cys Thr Val Ala Glu Trp Gly Val Arg
115 120 125
Thr Glu Leu Gly Val Asp Ala Lys Ser Val Leu Leu Thr Glu Thr Gly
130 135 140
His Ser Glu Ala Ser Ile Asp Ile Ser Val Ser Gly Glu Lys Asp Val
145 150 155 160
Glu Glu Gly Gly Asp Val Glu Met Thr Cys Arg Cys His Gly Cys Thr
165 170 175
Ser Ala Ala Ile Phe Asp Trp Phe Lys Gly Ala Phe Ala Gly Ser Glu
180 185 190
Trp Val Thr Thr Gly Asn Tyr Thr His Ile Ala Ala Lys Val Asp Val
195 200 205
Gly Val Leu Gly Phe Pro Asn Pro Ile Glu Ile Asp Asp Gly Phe Gly
210 215 220
Gln Phe Ser Val Thr Pro Ser Asn Ser Leu Arg Leu Thr Gly Ala Gln
225 230 235 240
Val Ala Asp Ala Gly Arg Tyr Trp Cys Lys Val Thr Ser Gly Gly Ser
245 250 255
Val Asp Ile Lys Ala Thr Val Leu Lys Val Lys Val Pro Glu Phe Thr
260 265 270
Cys Ala Gly Lys Ala Asp Gly Tyr Tyr Pro Asp Pro Glu Asp Cys Ala
275 280 285
Met Tyr Tyr Gln Cys Leu Tyr Gly Phe Pro Gln Pro Phe His Arg Pro
290 295 300
Cys Gly Tyr Ala Gly Met Val Phe Asn Pro Glu His Leu Tyr Cys Asp
305 310 315 320
Trp Ala Phe Asn Val Gly Pro Pro Cys Gly Ser Lys Ala
325 330
<210> 21
<211> 334
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 21
Met Gln Met Phe Leu Leu Val Ser Val Cys Leu Gly Met Ala Tyr Gly
1 5 10 15
Gln Ser Ile Met Thr Val Arg Thr Thr His Thr Glu Val Glu Val His
20 25 30
Ala Gly Gly Thr Val Glu Leu Pro Cys Ser Tyr Gln Leu Ala Asn Asp
35 40 45
Thr Gln Pro Pro Val Ile Ser Trp Leu Lys Gly Ala Ser Pro Asp Arg
50 55 60
Ser Thr Lys Val Phe Lys Gly Asn Tyr Asn Trp Gln Gly Glu Gly Leu
65 70 75 80
Gly Phe Val Glu Ser Asp Ser Tyr Lys Glu Ser Phe Gly Asp Phe Leu
85 90 95
Gly Arg Ala Ser Val Ala Asn Leu Ala Ala Pro Thr Leu Arg Leu Thr
100 105 110
His Val His Pro Gln Asp Gly Gly Arg Tyr Trp Cys Gln Val Ala Gln
115 120 125
Trp Ser Ile Arg Thr Glu Phe Gly Leu Asp Ala Lys Ser Val Val Leu
130 135 140
Lys Val Thr Gly His Thr Pro Ser Asn Asn Val His Val Ser Thr Ala
145 150 155 160
Glu Val Val Gln Val Asp Glu Gly Asn Asp Ile Thr Met Thr Cys Pro
165 170 175
Cys Thr Asp Cys Ala Asn Ala Asn Val Thr Trp Tyr Thr Gly Pro Thr
180 185 190
Phe Phe Glu Asn Tyr Glu Thr Gly Thr Tyr Gln Pro Leu Ala Asn Lys
195 200 205
Asn Gln Phe Gly Ile Thr Trp Phe Ser Ser Glu Ile Ala Gly Arg Ala
210 215 220
Ser Phe Ser Gly Ala Arg Asn Leu Val Ile Arg Ala Ala Lys Ile Thr
225 230 235 240
Asp Ala Gly Arg Val Trp Cys Glu Leu Ala Thr Gly Gln Gly Glu Leu
245 250 255
Asp Ala Asp Arg Ser Ser Thr Ile Leu Lys Val Gln Leu Glu Pro Phe
260 265 270
Thr Cys Asp Gly Lys Pro Thr Gly Leu Tyr Ala Asp Pro Thr Ala Cys
275 280 285
Asp Tyr Tyr Tyr Gln Cys Ile Pro Gly Tyr Pro Pro Leu His Arg Pro
290 295 300
Cys Gly Tyr Ala Gly Met Val Phe Asn Glu Glu Met Gln Tyr Cys Asp
305 310 315 320
Trp Asp Ile Asn Val Pro Pro Pro Cys Gly Ser Lys Pro Val
325 330
<210> 22
<211> 356
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 22
Met Ser Val Leu Leu Val Ser Ile Ile Leu Leu Leu Tyr Val Gly Pro
1 5 10 15
Glu Ser Ala Asp Ala Val Ser Ile Thr Thr Val Thr Val Pro Asp Arg
20 25 30
Gly Gly Trp Val Val Met Trp Pro Arg Pro Gly Asp Pro Thr Trp Val
35 40 45
Asn Arg Ile Glu Phe Arg Cys Glu Tyr Ser Ile Ser Pro Ala Ser Ala
50 55 60
Thr Pro Pro Thr Ile Thr Trp Leu Lys Gly Val Phe Ala Asp Ala Asp
65 70 75 80
Arg Gln Val Ile Tyr Lys Trp Ser Ser Ser Gly Glu Val Tyr Val His
85 90 95
Pro Glu Tyr Ala Gly Arg Val Ser Val Pro Ser Arg Thr His Pro Thr
100 105 110
Leu Val Leu Thr Asp Ala Lys Phe Asp Asp Trp Gly Arg Tyr Trp Cys
115 120 125
Arg Val Thr Asn Glu Asp Gln Ser Asp Glu Phe Gly Thr Asp Glu Glu
130 135 140
Ser Leu Leu Phe Trp Tyr Lys Leu Gly Tyr Asp Pro Pro Thr Arg Leu
145 150 155 160
Ser Gln Val Ser Leu Asp Lys Thr Pro Val Arg Val Ala Thr Gly Gly
165 170 175
Thr Ala Arg Leu Asp Cys Thr Gly Thr Ser Gly Arg Glu Ala Ser Ile
180 185 190
Leu Trp Val Lys Gly Pro Thr Ser Cys Thr Gln Gly Glu Ser Cys Asp
195 200 205
Ser Tyr Glu Thr Val Ile His Lys Ser Ala Val Trp Gly Ala Gly Asn
210 215 220
Pro Glu Pro Glu Asn Ile Thr Ile Ser Pro Ser Phe Asp Gly Arg Val
225 230 235 240
Ser Leu Asp Ala Asp Gly Ser Gly Ser Phe Thr Pro Thr Leu Thr Ile
245 250 255
Thr Asp Ile Arg Pro Ser Asp Ser Gly Arg Tyr Trp Cys Ala Pro Asp
260 265 270
Ile Ser Glu Val Tyr Ser Asn Leu Gly Leu Leu Asn Arg Asp Ala Gln
275 280 285
Ser Val Val Ile Ile Val Asn Asp Pro Gly Thr Glu Pro Thr Cys Ala
290 295 300
Gly Lys Pro Asp Gly Met Tyr Gln His Pro Ala Asp Cys Ala Gln Phe
305 310 315 320
Tyr Thr Cys Ser Gly Gly Leu Ser Tyr Gly Thr Asn Asn Cys Pro Ala
325 330 335
Gly Leu Val Phe Asn Gln Glu Leu Gln Leu Cys Asp Trp Ala Asn Asn
340 345 350
Val Ile Cys Leu
355
<210> 23
<211> 528
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 23
Met Ala Leu Leu Ala Val Leu Leu Phe His Ala Ala Leu Trp Ala Ala
1 5 10 15
Gly Glu Ala Gln Gly Thr Tyr Leu Gly Cys Tyr Ile Asp Thr Pro Asn
20 25 30
Asp Arg Asp Phe Pro His Val Thr Thr Met Thr Tyr Thr Asn Gly Tyr
35 40 45
Thr Thr Cys Ser Gln Tyr Cys Ala Ser Leu Gly Tyr Ser Tyr Ile Gly
50 55 60
Leu Gln Tyr Ala Ile Glu Cys Phe Cys Gly His Thr Tyr Gly Lys His
65 70 75 80
Gly Arg Ala Ala Glu Ser Asp Cys Tyr Tyr Gly Cys Ser Gly Gly Thr
85 90 95
Gly Gly Lys Cys Gly Gly Asp Tyr Arg Asn Thr Val Trp Asp Ile Gly
100 105 110
Asp Val Gly Gly Gly Ser Thr Gly Thr Gly Thr Ser Asp Asn Leu Glu
115 120 125
Leu Val Phe Glu Asp Thr Phe Asp Thr Leu Asp Thr Thr Lys Trp Arg
130 135 140
Pro Glu Val Thr Ala Gly Gly Gly Gly Asn Glu Glu Phe Gln Tyr Tyr
145 150 155 160
Met Gly His Pro Asp Asn Leu Tyr Val Gln Asn Gly Asn Leu Tyr Leu
165 170 175
Lys Pro Thr Leu Ala Ser Glu His Tyr Gly Asp Asn Phe Met Thr Ser
180 185 190
Gly Tyr Ile Asp Phe Trp His Gly Tyr Asn Tyr Val Gly Cys Phe Asn
195 200 205
Asp Gly Gly Asp Ala Asp Leu Thr Glu Ser Leu Leu Thr Ala Ser Asp
210 215 220
Met Asn Pro Gly Lys Cys Val Thr His Cys Ala Asn Gly Gly Tyr Glu
225 230 235 240
Tyr Phe Gly Leu Lys Asn Thr Glu Cys Arg Cys Gly Asn Ser Phe Gly
245 250 255
Arg Phe Gly His Gly His Gly Cys His Ile Ser Cys Pro Gly Asp Ser
260 265 270
Gly Lys Glu Cys Gly Gly Asn Gly Val Thr Ser Val Tyr Gly Gln Thr
275 280 285
Tyr Gly Asp Cys Thr Ser Asp Phe Pro Gln Gly Arg Ala Cys Tyr Leu
290 295 300
Gln Gly Ser Gln Asn Ala Pro Leu Pro Pro Ile Gln Ser Ala Arg Val
305 310 315 320
Thr Thr Val Glu Ser Phe Ala Phe Lys Tyr Gly Lys Val Glu Val Trp
325 330 335
Ala Lys Leu Pro Thr Gly Asp Trp Ile Trp Pro Ala Ile Trp Leu Leu
340 345 350
Pro Arg Asp Asn Val Tyr Gly Gly Trp Pro Ala Ser Gly Glu Ile Asp
355 360 365
Ile Met Glu Ser Arg Gly Asn Arg Asn Leu Tyr Gly Ser Trp Gly Gly
370 375 380
Ser Ile Gly Val Asp Asp Met Gly Ser Thr Leu His Trp Gly Pro Arg
385 390 395 400
Trp Pro Tyr Asn Gly Phe Gln Gln Thr Thr Ala Ser Lys Val Glu Val
405 410 415
Asp Asp Gln Glu Val Leu Leu Val Ser Pro Pro Thr Asn Phe Trp Asp
420 425 430
Met Gly Asn Phe Gly Leu Pro Ser Tyr Glu Asn Pro Trp Ala Gly Gly
435 440 445
Gly Lys Met Thr Pro Phe Asp Arg Glu Phe Tyr Leu Ile Phe Asn Val
450 455 460
Ala Val Gly Gly Thr Asn Tyr Tyr Phe Pro Asp Asp Ala Thr Asn Gln
465 470 475 480
Pro Tyr Pro Lys Pro Trp Ser Asn Asn Asn Ser Arg Glu Gly Ala Met
485 490 495
Arg Asp Phe Trp Asn Ala Lys Asn Gln Trp Tyr Pro Thr Trp Asn Gly
500 505 510
Asp Asp Val Ala Met Gln Ile Gln Ser Ile Lys Val Trp Arg Phe Val
515 520 525
<210> 24
<211> 1530
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 24
Met Arg Gly Leu Leu Leu Leu Cys Gly Val Leu Gly Ser Val Pro Ala
1 5 10 15
Gln Lys Leu Asp Pro Thr Pro Gly Pro Thr Pro Gly Thr Pro Gly Thr
20 25 30
Phe Leu Ile Met Ala Pro Lys Val Val Arg Pro Gly Arg Pro Leu Thr
35 40 45
Leu Ser Val Ser Ile Leu Asp Pro Ala Ala Ala Ser Val Gln Val Thr
50 55 60
Ala Thr Leu Leu Gln Asn Gly Arg Pro Thr Gly Thr Pro Val Ser Gly
65 70 75 80
Asp Phe Ser Arg Asn Ala Asp Gly Thr Leu Asp Leu Val Val Pro Gly
85 90 95
Ala Leu Thr Ala Gly Ser Ala Tyr Lys Leu Lys Val Glu Gly Thr Gly
100 105 110
Gly Leu Thr Phe Thr Glu Glu Lys Asp Leu Thr Phe Asn Ser Lys Ser
115 120 125
Ser Ser Ile Phe Val Gln Thr Asp Lys Gly Lys Tyr Lys Pro Gly Gln
130 135 140
Thr Val Lys Met Arg Ala Leu Ala Val Asp Ala Glu Leu Lys Thr Lys
145 150 155 160
Lys Glu Pro Phe Asp Ile Asp Ile Phe Asp Pro Gln Gly Asn Glu Ile
165 170 175
Val Gln Trp Arg Gly Leu Gln Asn Asp Asp Gly Leu Val Glu Lys Glu
180 185 190
Phe Ala Leu Ser Asp Gln Pro Leu Leu Gly Asp Trp Lys Ile Val Ala
195 200 205
Met Leu Pro Gly Gly Asp Glu Val Glu Gln Pro Phe Gly Val Glu Glu
210 215 220
Tyr Val Leu Pro Lys Phe Glu Val Thr Ile Ser Thr Asn Ser Phe Phe
225 230 235 240
Ala Arg Thr Glu Asp Thr Leu Gln Gly Thr Phe Asn Ala Lys Tyr Thr
245 250 255
Tyr Gly Lys Asn Val Glu Gly Gln Gly Lys Val Thr Val Lys Leu Arg
260 265 270
Ser Val Gly Ser Asn Phe Arg Pro Thr Asp Asn Ser Ile Glu His Asn
275 280 285
Asn Ile Ala Met Val Glu Gly Ser Gly Ser Phe Gln Phe Thr Arg Gly
290 295 300
Asp Ile Glu Gly Leu Tyr Gly Asn Pro Asp Gln Leu Tyr Tyr Gly Ser
305 310 315 320
Tyr Gln Leu Glu Leu Ile Val Glu Val Thr Glu Gly Leu Thr Gly Asn
325 330 335
Thr Gln Glu Ala Thr Lys Glu Leu Asp Leu Val Gln Thr Lys Tyr Lys
340 345 350
Leu Glu Ser Phe Asn Thr Pro Asp Asn Phe Lys Pro Gly Leu Gln Tyr
355 360 365
Thr Ala Tyr Val Arg Thr Ser Tyr His Asp Gly Thr Pro Met Leu Gly
370 375 380
Met Asp Leu Ala Gln Pro Val Thr Leu Ser Lys Asn Tyr Gly Trp Asp
385 390 395 400
Gly Pro Thr Ala Pro Pro Ala Glu Glu Leu Thr Ile Pro Gly Ser Gly
405 410 415
Val Ile Lys Ile Val Met Asp Ala Pro Ser Thr Ala Asn Ser Met Thr
420 425 430
Leu Ser Val Met Tyr Asn Asp Ile Ser Glu Thr Ala Thr Thr His Thr
435 440 445
Ala Gln Lys Ala Glu Ser Pro Ser Ser Ser Tyr Ile Gln Val Thr Thr
450 455 460
Ser Thr Pro Glu Val Thr Ala Gly Glu Asp Ala Gln Phe Thr Val Lys
465 470 475 480
Ser Thr Ser Pro Ile Gly Tyr Phe Ser Tyr Gln Val Leu Ser Arg Gly
485 490 495
Asn Ile Val Val Val Asp Arg Val Glu Val Thr Pro Glu Asp Thr Leu
500 505 510
Lys Ser Phe Thr Val Pro Thr Asn Thr Gln Met Ala Pro Ser Ser Arg
515 520 525
Met Val Val Tyr Trp Met Thr Asp Thr Gly Glu Ile Cys Asn Asp Gly
530 535 540
Leu Thr Phe Thr Val Lys Gly Ala Phe Glu Asn Gln Val Ser Leu Ser
545 550 555 560
Tyr Asp Lys Asp Arg Ala Glu Pro Ala Glu Asp Ile Ala Val Thr Val
565 570 575
Arg Ala Asp Pro Asp Ser Leu Val Ala Leu Leu Thr Val Asp Gln Gly
580 585 590
Val Leu Leu Leu Gln Ser Gly Asn Asp Ile Thr Gln Asp Lys Val Leu
595 600 605
Ala Glu Leu Glu Glu Tyr Asp Pro Ala Glu Asp Thr Ser Asn Ser Gly
610 615 620
Gly Pro Glu Ile Met Trp Arg Arg Arg Lys Arg Ser Ile Trp Trp Pro
625 630 635 640
Met Pro Thr Ser Gly Asp Asp Ala His Ser Val Phe Glu Asn Ala Gly
645 650 655
Val Ile Val Leu Thr Asp Ala Met Val Tyr Gln Lys Gln Pro Asp Tyr
660 665 670
Trp Trp Gln Trp Gly Ile Leu Pro Val Ala Asp Phe Asp Leu Gly Met
675 680 685
Met Glu Phe Met Pro Ala Ala Ala Ala Gly Glu Ser Ala Gly Gly Gly
690 695 700
Gly Gly Arg Gly Gly Leu Gln Glu Val Lys Arg Val Arg Ser Phe Phe
705 710 715 720
Pro Glu Thr Trp Leu Trp Thr Asn Ala Thr Ala Gly Ala Asp Gly Thr
725 730 735
Ala Val Phe Ala Ser Thr Val Pro Asp Thr Ile Thr Ser Trp Val Ala
740 745 750
Ser Ala Phe Ala Leu Asn Asp Val Ser Gly Leu Gly Val Ala Asp Thr
755 760 765
Pro Ala Ser Met Glu Ala Phe Arg Pro Phe Phe Val Ser Leu Asn Leu
770 775 780
Pro Tyr Ser Val Val Asn Lys Glu Glu Thr Ala Ile Gln Ala Leu Val
785 790 795 800
Phe Asn Tyr Met Asp Val Asp Met Asp Val Thr Val Thr Leu Lys Gly
805 810 815
Ser Ser Gly Phe Met His Ile Val Met Asp Thr Asn Asp Val Asn Ser
820 825 830
Ile Gly Thr Glu Val Ala Gly Asp Gln Ala Val Val Leu Thr Val Pro
835 840 845
Ala Gly Ser Ser Arg Ser Lys Ser Phe Pro Ile Ile Pro Lys Gln Phe
850 855 860
Gly Ala Ile Thr Val Lys Val Leu Ala Gln Ser Thr Leu Ala Ala Asp
865 870 875 880
Ala Val Glu Arg Gln Leu Leu Val Glu Pro Glu Gly Glu Gln Lys Glu
885 890 895
Tyr Thr Lys Ser Phe Ile Leu Asp Leu Gly Asp Ser Gly Asn Gly Asp
900 905 910
Ile Thr Lys Asp Leu Tyr Leu Ser Val Pro Gln Ser Gly Leu Val Ala
915 920 925
Gly Ser Ala Tyr Ala Arg Val Ser Val Met Gly Asp Met Met Gly Ser
930 935 940
Thr Ile Ser Gly Leu Asp Ser Leu Leu Gln Met Pro Tyr Gly Cys Gly
945 950 955 960
Glu Gln Thr Met Ile Thr Phe Ala Pro Asn Val Tyr Val Met His Tyr
965 970 975
Leu Asn Glu Thr Asn Gln Val Thr Pro Glu Ile Lys Ser Lys Ala Leu
980 985 990
Lys Phe Met Thr Ser Gly Tyr Gln Arg Glu Leu Thr Tyr Thr His Asp
995 1000 1005
Asp Gly Ser Phe Ser Ala Phe Gly Ser Ser Asp Ala Ser Gly Ser Thr
1010 1015 1020
Trp Leu Ser Ala Phe Val Met Lys Ser Phe Ser Gln Ala Met Asp Tyr
1025 1030 1035 1040
Ile Tyr Ile Asp Pro Lys Val Leu Ala Lys Thr Ile Arg Trp Leu Ile
1045 1050 1055
Arg Gln Gln Gln Asp Thr Gly Val Phe Ser Glu Pro Gly Asn Val Ile
1060 1065 1070
His Lys Glu Met Gln Gly Gly Leu Ser Ser Asp Val Thr Met Thr Ser
1075 1080 1085
Tyr Val Leu Val Ala Leu Met Glu Tyr Asp Lys Ile Leu Ala Thr Leu
1090 1095 1100
Asp Ala Pro Asp Ser Asp Lys Val Asp Val Thr Ile Ala Lys Asn Arg
1105 1110 1115 1120
Ala Val Ala Phe Leu Glu Ser Arg Leu Asp Ser Glu Thr Asp Val Tyr
1125 1130 1135
Thr Ile Val Thr Met Thr Tyr Ala Leu Thr Leu Ala Gly Ser Arg Arg
1140 1145 1150
Lys Asp Ala Ala Phe Gln Lys Met Asn Ser Leu Ala Thr Thr Glu Ala
1155 1160 1165
Gly Gln Met Tyr Trp Thr Lys Gly Glu Ser Ala Thr Ser Pro Pro Val
1170 1175 1180
Ser Asp Asp Leu Met Trp Thr Pro Pro Tyr His Asn Pro Pro Ser Ala
1185 1190 1195 1200
Asp Val Glu Met Thr Ala Tyr Ala Leu Leu Thr Tyr Leu Asp Arg Asp
1205 1210 1215
Asp Leu Val Ala Gly Met Pro Ile Met Lys Trp Leu Thr Glu Gln Arg
1220 1225 1230
Asn Ala Tyr Gly Gly Tyr Ser Ser Thr Gln Asp Thr Val Val Gly Leu
1235 1240 1245
Gln Ala Leu Gly Tyr Phe Ala Ala Ala Val Arg Ala Gly Gly Leu Asp
1250 1255 1260
Met Thr Val Thr Ile Thr Ser Asp Thr Asp Thr Asp Phe Ser His Thr
1265 1270 1275 1280
Phe Thr Val Asn Asp Gln Asn Ala Ile Val Leu Gln Arg Val Glu Ile
1285 1290 1295
Pro Asn Val Ser Gly Thr Leu Thr Ala Arg Ala Gln Gly Gln Gly Met
1300 1305 1310
Gly Leu Leu Gln Leu Phe Val Arg Tyr Asn Val Asp Val Val Glu Ala
1315 1320 1325
Val Pro Ala Phe Asn Leu Ile Ala Thr Val Lys Asn Ser Asp Thr Asn
1330 1335 1340
Ser Val Thr Cys Glu Thr Cys Gly Thr Tyr Gln Leu Gly Gly Ser Ser
1345 1350 1355 1360
Gly Met Ala Val Met Glu Ile Gly Ile Pro Ser Gly Phe Tyr Val Glu
1365 1370 1375
Asp Ala Asp Leu Met Ala Leu Val Asp Asn Gln Ala Met Pro Thr Leu
1380 1385 1390
Lys Arg Ala Glu Arg Ser Thr Asp Asn Lys Arg Ala Val Ile Tyr Phe
1395 1400 1405
Asp Glu Met Asn Ala Ser Pro Thr Cys Val Thr Leu Thr Ser His Arg
1410 1415 1420
Ser Met Pro Val Ala Lys Thr Gln Pro Ser Val Val Lys Val Tyr Asp
1425 1430 1435 1440
Tyr Tyr Lys Pro Asp Val Lys Ala Ser Asn Ile Tyr Gln Ser Glu Thr
1445 1450 1455
Leu Lys Asn Thr Asn Ile Cys Glu Val Cys Ala Asp Cys Thr Gly Cys
1460 1465 1470
Ser Gly Arg Gln Gly Arg Pro Arg Ser Ala Ala Ala Gly Leu Ala Val
1475 1480 1485
Asp Thr Leu Leu Gly Val Val Leu Leu Leu Leu Thr Arg Leu Leu Val
1490 1495 1500
Arg Gln Cys Arg Pro Thr Asn Thr Cys Glu Glu Cys Leu Asn Val Pro
1505 1510 1515 1520
Cys Ser Glu Leu Val Thr Lys Leu Arg Leu
1525 1530
<210> 25
<211> 500
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 25
Met Leu Thr Val Leu Ala Leu Leu Cys Val Met Leu Ser Leu Gly Gln
1 5 10 15
Ala Ser Val Ile Pro Ala Gln Arg Asn Thr Tyr Arg Arg Val Cys Tyr
20 25 30
His Thr Asn Trp Ser Gln Tyr Arg His Gly Val Gly Gln Phe Phe Pro
35 40 45
Glu Asp Ile Asp Pro Thr Leu Cys Thr His Ala Ile Tyr Ala Phe Ala
50 55 60
Lys Met Thr Asn Asn Gln Leu Gln Pro Tyr Glu Trp Asn Asp Asp Ser
65 70 75 80
Thr Asp Trp Ser Thr Gly Met Tyr Glu Arg Phe Asn Thr His Leu Lys
85 90 95
Pro His Gly Val Lys Thr Leu Leu Gly Val Gly Gly Trp Asn Phe Gly
100 105 110
Ser Thr Ala Phe Ser Asp Met Ala Ala Thr Ala Ala Gly Arg Arg Thr
115 120 125
Phe Val Thr Thr Ser Ile Asp Phe Leu Arg Gln Arg Asn Phe Asp Gly
130 135 140
Leu Asp Leu Asp Trp Glu Tyr Pro Ala Ala Arg Gly Gly Arg Pro Ser
145 150 155 160
Asp Lys Gln Thr Phe Thr Leu Leu Arg Lys Glu Leu Arg Glu Ala Phe
165 170 175
Asp Ala Glu Gly Ala Arg Thr Gly Arg Ala Pro Leu Leu Leu Thr Ala
180 185 190
Ala Met Pro Ala Gly Glu Asp Asn Ala Met Asn Gly Tyr Glu Met Ala
195 200 205
Glu Val Ala Gly Tyr Leu Asp Phe Leu Asn Leu Met Ala Tyr Asp Leu
210 215 220
His Gly Gln Trp Glu Thr Tyr Thr Gly Leu Asn Ser Pro Leu Tyr Ala
225 230 235 240
Ala Gly Asp Glu Thr Gly Asp Asp Arg Lys Leu Asn Gln Ala Phe Ala
245 250 255
Val Asp Met Trp Leu Ala Gly Gly Val Pro Pro Ser Lys Leu Asn Leu
260 265 270
Gly Met Gly Thr Tyr Gly Arg Ser Phe Thr Thr Thr Gly Asp Asn Ser
275 280 285
Ile Arg Ala Pro Ala Asn Gly Gly Gly Asn Ala Gly Leu Tyr Thr Arg
290 295 300
Glu Lys Gly Tyr Leu Ala Tyr Tyr Glu Ile Cys Thr Met Leu Asn Gln
305 310 315 320
Gly Ala Thr Arg Val Phe His Ser Glu His Leu Ser Pro Tyr Ala Tyr
325 330 335
Leu Gly Asn Gln Trp Val Gly Tyr Asp Asp Val Glu Ser Leu Thr Tyr
340 345 350
Lys Val Glu Tyr Leu Lys Ser Lys Asn Leu Gly Gly Ala Met Val Trp
355 360 365
Ala Tyr Asp Ile Asp Asp Phe Ser Gly Ser Ser Cys Gly Gln Gly Arg
370 375 380
Tyr Pro Leu Met Asn Ala Ile Lys Asn Leu Leu Glu Thr Gly Ser Ala
385 390 395 400
Gly Val Val Leu Pro Pro Gly Pro Thr His Pro Pro Ile Asn Pro Ala
405 410 415
Thr Gln Ala Pro Asn Pro Gly Val Thr Gln Gly Pro Asn Pro Val Val
420 425 430
Thr Gln Ala Pro Val Val Val Glu Gln Gly Asn Leu Ala Ala Val Thr
435 440 445
Cys Asp Ser Lys Pro Asp Gly Phe His Pro Asp Pro Thr Asp Cys Gly
450 455 460
Lys Tyr Phe Gln Cys Trp Gly Gly Thr Met Trp Pro Gly His Cys Ser
465 470 475 480
Asn Gly Leu Gln Trp Asn Gln Ala Met Leu Gly Cys Asp Trp Pro Tyr
485 490 495
Asn Val Asn Cys
500
<210> 26
<211> 117
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 26
Met Glu Lys Lys Thr Ala Tyr Cys Leu Leu Phe Leu Val Leu Leu Val
1 5 10 15
Pro Tyr Thr Ala Leu Gly Ala Val Leu Lys Arg Ala Pro Ala Lys Lys
20 25 30
Glu Lys Arg Ala Val Pro Leu Ala Val Pro Leu Val Tyr Trp Gly Ala
35 40 45
Ser Val Ser Pro Ala Val Trp Asn Trp Leu Leu Val Thr Phe Gly Ala
50 55 60
Ala Ala Val Ala Ala Ala Ala Val Thr Val Ser Asp Asn Asp Ser His
65 70 75 80
Ser Cys Ala Asn Asn Arg Gly Trp Cys Arg Ser Arg Cys Phe Ser His
85 90 95
Glu Tyr Ile Asp Ser Trp His Ser Asp Val Cys Gly Ser Tyr Asp Cys
100 105 110
Cys Arg Pro Arg Tyr
115
<210> 27
<211> 774
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 27
Met Gly Arg Phe Tyr Ala Trp Leu Val Leu Ala Ile Val Ala Phe Ser
1 5 10 15
Ser His Gly Arg Ala Glu Asp Gly Glu Asp Gln Pro Gly His Phe Thr
20 25 30
Asn Thr Trp Ala Val Glu Ile Tyr Gly Gly Pro Asp Arg Ala Asp Leu
35 40 45
Leu Ala Leu Asp His Gly Tyr Glu Asn Leu Gly Gln Ile Gly Asn Leu
50 55 60
Glu Asp His Tyr Leu Phe Arg His Lys Asp Val Pro His Arg Ser Arg
65 70 75 80
Arg Gly Ala His Gln His Thr Lys Arg Leu Gly Asp Asp Glu Arg Ile
85 90 95
Gln Trp Val Ala Gln Gln Val Gly Arg Ala Arg Ser Lys Arg Gly Pro
100 105 110
Met Gly Gln Gln Arg Arg Gln Ser Asp Asp Thr Arg Pro Met Thr Phe
115 120 125
Arg Asp Pro Tyr Trp Glu Lys Gln Trp Tyr Leu His Asp Thr Arg Thr
130 135 140
Ser Thr Asn Leu Pro Lys Leu Asp Leu His Val Leu Pro Val Trp Arg
145 150 155 160
Lys Gly Ile Thr Gly Lys Gly Ile Val Val Ala Val Leu Asp Asp Gly
165 170 175
Ile Glu Lys Asp His Pro Asp Leu Val Asp Asn Tyr Asp Pro Asp Ala
180 185 190
Ser Tyr Asp Phe Asn Asp Asn Asp Asp Asp Pro Gln Pro Arg Tyr Glu
195 200 205
Glu Thr Asn Glu Asn Lys His Gly Thr Arg Cys Ala Gly Glu Ile Ala
210 215 220
Met Ala Ala Asn Asn Ser Glu Cys Gly Val Gly Ile Ala Phe Asn Ala
225 230 235 240
Arg Ile Gly Gly Val Arg Met Leu Asp Gly Val Val Thr Asp Ala Val
245 250 255
Glu Ala Ser Ser Ile Gly Phe Asn Ile Gln His Val Asp Ile Tyr Ser
260 265 270
Ala Ser Trp Gly Pro Asn Asp Asp Gly Lys Thr Val Glu Gly Pro Glu
275 280 285
Lys Leu Ala Arg Ala Ala Phe Glu Lys Gly Val Arg Glu Gly Arg Gly
290 295 300
Gly Lys Gly Ser Ile Phe Ala Trp Ala Ser Gly Asn Gly Gly Ser Asn
305 310 315 320
Gly Asp Asn Cys Asp Cys Asp Gly Tyr Thr Ser Ser Ile Tyr Thr Val
325 330 335
Ser Ile Ser Ser Ala Ser Gln Gln Gly Gly Ser Pro Trp Tyr Gly Glu
340 345 350
Lys Cys Ala Ser Thr Leu Ala Thr Ala Tyr Ser Ser Gly Glu Tyr Lys
355 360 365
Asp Gln Lys Ile Ser Ser Thr Asp Leu His His Glu Cys Thr Asp Ser
370 375 380
His Thr Gly Thr Ser Ala Ala Ala Pro Leu Ala Ala Gly Val Leu Ala
385 390 395 400
Leu Ala Leu Glu Ala Asn Pro Asn Leu Thr Trp Arg Asp Val Gln His
405 410 415
Leu Ile Val Trp Thr Ser Glu Tyr Asp Pro Leu Ser Ser Asn Pro Gly
420 425 430
Trp Phe Gln Asn Gly Ala Gly Leu Trp Val Asn Ser Arg Phe Gly Tyr
435 440 445
Gly Leu Leu Asn Ala Glu Ala Met Val Asp Met Ala Leu Thr Trp Lys
450 455 460
Thr Val Pro Glu Lys Thr Lys Cys Glu Val Arg Ile Glu Asn Phe Gln
465 470 475 480
Pro Arg Asp Leu Gly Asn Gly Glu Glu Ile Ile Ile Glu Leu Glu Thr
485 490 495
Asp Gly Cys Arg Gly Gln Asn His Val Glu Ala Leu Glu His Val Gln
500 505 510
Val Lys Thr Thr Ile Asp Tyr Ser Arg Arg Gly Asp Leu Arg Ile Val
515 520 525
Leu Thr Ser Pro Ser Gly Thr Ser Thr Thr Leu Leu Asp Thr Arg Arg
530 535 540
Gln Asp Lys Ser Gln Met Gly Phe Gln Asp Trp Pro Phe Met Ser Thr
545 550 555 560
His Asn Trp Gly Glu Lys Pro Gln Gly Lys Trp Thr Leu Thr Ile Glu
565 570 575
Asp Lys Ser Asp His Ala Glu Asn Asn Gly Val Val Lys Asp Val Val
580 585 590
Leu Ile Leu His Gly Thr Pro Glu Gln Pro Ala Tyr Gln Ser Gly Gly
595 600 605
Arg Val Tyr Thr Asp Tyr Asn Arg Val Gln Gly Asp Arg Asn Val Glu
610 615 620
Ile Ser Ala Ala Lys Ala Ala Pro Ala Pro Ala Ala Asp Ala Ala Pro
625 630 635 640
Ala Arg Gly Ser Glu Glu Arg Ile Pro Gly Ser Gly Pro Phe Gly Ser
645 650 655
Ala Ala Ser Val Val Ile Glu Glu Ile Pro Glu Lys Glu Thr Glu Phe
660 665 670
Leu Val Asn Trp Gln Asp Gly Met Asn Arg Tyr Glu Thr Asp Phe Asn
675 680 685
Pro Val Asn Ser Gly Pro Phe Glu Pro Ser Ala Asp Ala Gly Ser Asp
690 695 700
Val Tyr Met Asp Ala Glu Asp Leu Arg Pro Trp Glu Ala Phe Lys Tyr
705 710 715 720
Leu Gln Arg Gln Met Lys Ser Pro Ser Gly Gln Arg His Pro Ala His
725 730 735
Ala Tyr Asn Lys Pro Ser Ser Gln Tyr Ile Arg Asp Pro Trp Val Ser
740 745 750
Gln Asn Ala Leu Asp Lys Glu Ala Asn Leu Val Lys Tyr Tyr Leu Gln
755 760 765
Leu Leu Gly Tyr Glu Ser
770
<210> 28
<211> 271
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 28
Met Ala Thr Lys Val Thr Lys Arg Gly Gln Val Met Leu Gly Asp Leu
1 5 10 15
Pro Asp Asp Phe Leu Arg Ile Glu Ala Thr Pro Gln Gln Leu Gln Ala
20 25 30
Thr Ala Asp Met Gln Ala Ala Gln Met Tyr Ala Gln Tyr Gly Phe Met
35 40 45
Gly Gln Ser Ser Gly Arg Leu Ser Ile Thr Val Ala Gln Ala Lys Leu
50 55 60
Ala Lys Asn Tyr Gly Val Thr Arg Met Asp Pro Tyr Cys Arg Ile Arg
65 70 75 80
Val Gly His Ala Val Phe Glu Thr Pro Thr Ala Tyr Asn Gly Ala Lys
85 90 95
Asn Pro Arg Trp Asn Lys Val Val His Ala Pro Ile Pro Gln Gly Val
100 105 110
Asn Ser Phe Tyr Leu Glu Ile Phe Asp Glu Arg Ser Phe Thr Met Asp
115 120 125
Asp Arg Val Ala Trp Gly His Ile Thr Ile Pro Glu Ser Val Phe Asn
130 135 140
Gly Glu Thr Val Asp Asp Trp Tyr Thr Leu Ser Gly Arg Gln Gly Asp
145 150 155 160
Asp Lys Glu Gly Met Ile Asn Leu Val Leu Ser His Ser Thr Val Pro
165 170 175
Met Met Gln Gln Val Pro Gln Val Met Pro Gln Met Pro Met Met Met
180 185 190
Val Pro Gln Pro Val Tyr Tyr Pro Thr Val Gln Ala Gly Val Pro Tyr
195 200 205
Gln Val Pro Ala Gly Gln Met Pro Ala Gln Gln Gln Pro Pro Ala Gln
210 215 220
Pro Ala Ile Thr Glu Glu Asp Leu Lys Ala Leu Gln Asp Met Phe Pro
225 230 235 240
Asn Met Asp Ala Glu Val Val Arg Ser Val Leu Glu Ala Asn Arg Gly
245 250 255
Asn Lys Asp Ala Ala Val Asn Ser Leu Leu Ser Ile Thr Ala Glu
260 265 270

Claims (5)

1. a kind of biological agent for algae of degrading, it is characterised in that effective component is specificity in the epithelial cell of lancelet coecum The protein of expression.
2. a kind of biological agent for algae of degrading, it is characterised in that effective component is made of following protein: cathepsin L, cathepsin B, cathepsin D, ferritin, trypsin-like serine protease, lysozymes C, lysozymes G, carboxypeptidase Z/N, tetraspanin, legumain, saposin B, subtilisin-like protease, arylsulfatase B, endo-beta-1,4-glucanase, methionine adenosyltransferase, pancreatic lipase-like protein, plasminogen, peroxiredoxin V, VCBP4, VCBP1, VCBP3, VCBP5, Gram-negative bacteria-binding protein, alpha2-macroglobulin, chitotriosidase 1-like protein, big defensin, Proprotein convertase subtilisin/kexin type 1, Toll-interacting protein；Protein Correlated series it is as follows:
>cathepsin L
MRLLIAVVCVAMATASPLMNPQWEVFKKAYNRVYAAEEEFARRLIFEDNLKTIQMHNEEADRGLHTFRLGVN QYADMTHKEFLENVIGGCLLDTNTSKSTADHVHEYDPTLTDLPDTVDWRDKGYVTPVKNQEQCGSCWAFSTTGSLE GQHFKSTQKLVSLSEQNLVDCSRKRGTGLPGRLMDQGFKYIKDNGGIDTEECYPYKAKNEKCNYQASCSGATLTAK RRQDEGRGALQQAVATVGPISVAIDAGHSSFQLYQSGVYHKFFCSETKMDHGVLAVGYGTEEGKDYWLVKNSWGAS WGEKGYIKMSRNRHNNWGIATSASYPTV；
>cathepsin B
MLAWVVLSVLAAVSAKEFPIHQPLTQEIIDYVNTIDTTWKAGWNFQGATVSYVKGLCGVIRDPNNHKLPLKL HELNAQDIPDTFDSRTQWANCPTIKEVRDQGSCGSCWALAAVEAMSDRICVASKGSTMAHISAEDLNSCCKSCGNG CNGGFPEAAWEYWKRDGLVTGGPYGSHQGCQPYEIKPCEHHINGSRPACGKLEPTPRCKKSCESGYNVTFAKDKHY AKTAYSVSSKVQQIQMEIMTNGPVEAAFTVYADFPHYKSGVYQHESGAELGGHAVKMIGWGTEGSTPYWLIANSWN TDWGNMGFFKILRGQDECGIERDIVAGEPKLD；
>cathepsin D
MKFLSVLFALVVFASALHRIPLTKMKTVRRQLADVGITYDQVLDKDYSGKYYNIKDAPEPLTNYLDAQYYGP ISIGTPAQNFQVVFDTGSSNLWVPSKKCKLSDIACLLHNKYDSTQSSTYMKNGTDFAIRYGSGSLTGFLSEDTVTI GGLKVQNQTFAEAVTQPGITFVAAKFDGILGMGYDTISVDGVVPPFYNMVQQKLVDKPVFSFYLNRDPSSTTRGEL LLGGTDPKYYTGDFTFLDVTKPGYWQFKMDGIMINGKATDYCKGGCAAIADTGTSLIAGPTTEVQALNKQIGATPI PGGEYMVDCSQVSSLPPISFMLGGKAFELQGKDYVLQVTTMGQTVCVSGFLGIDVPAGPLWILGDVFIGPYYTLFD MGNNRVGFAPTAHVNTTRV；
>ferritin
MHAMMKTLTFVLLVLQCTLGARREDNQWLISREDAGDPDNQDFARQNFHEDCEAGINKQINLEMFAGYTYRS MASYFNRDDVALKGVADFFRHHSEEELEHAQLLEEFQNKRGGRVVYENLRKPEKDTWGSALEAMQAALTLEKNVNQ RLINLHKTAGQHNDMQMQDFLDSHFMTEQVEGIKQIADYITNIKRVGTSGWASTCSTSTPSAAASRGNSVRRTREE RNLHRTKSYL；
>trypsin-like serine protease
MIWCLLLLALAARGDAWLFSCSASDFQCSNGNCIPGSWECDGEADCSDGSDEASCSGGGTCQQGVHFFCANG LYCIDSNWVCDGDNDCGDMSDEQNCGGGSTGTTGTTAPITSFTGDCGQPAISPQNVRVVGGVQAVQGSWPWQASLK LYGGHVCGGQIIAPNWIVTAAHCVDGQSNPSQWRVSLGSHRRTSTDSTQQDFSVTRIIMHESYDSNRINNDVALMK LSGNAQFNNYVSPICLPTQDVAAGTNCVTTGWGDTGSGASTYLMQATVPIMEWNKCNSAQYMNGAITDKMICAGYD QGGKDACQGDSGGPLVCNYSGKWTLDGIVSWGYGCAQAYKPGIYTRVTQFVSWINNKMASN；
>lysozyme C
MFLAVVLVMGLVCSAHAKTYERCELARELVSRGLTSRSQAGEWICLVQHESSFRTGARGGPNWDGSYDHGLF QINDHYWCDNGGPHNDCGVSCSNLRDNNIADDVRCAKLIYQRHGFSAWYGWQNNCQGSTSSYVSGCF；
>lysozyme G
MMFLVILAIVGVATADDWQCTTSVAANGQNGQCAHVDTCPYHYFVSNKCPSYGNDVKCCYNCHLGGCSISGG SSGGTGGSGSGSYGNIMEVDTTGASSQTASQDNIWYSGVSASHQLASNDLGRLNNYKSQIFDAGNAKNMDAAVIAA IISRESRAGAALAADGTGDNGNGFGLMQVDYRYHTPAGGPYSTEHMMQGTQILIDTINCVKRNHPNWNDNMTLKGG ISGYNAGCGNVQTYAGMDGGTTGEDYANDVVARAQWLKGQGF；
>carboxypeptidase Z/N
MIFAILLAALPALALAEVDSSFVFEYHRYTALRSVLLAVSQDCPDITRLYSIGQSVQGRELLVLEISDNPGQH ELGEPEFKYVGNMHGNEVRGRELIILLAQYLCGEYKAGNSRIVSLVRDTRIHLMPTMNPDGFEVAANQGPDNNGWTT GRNNMQGIDLNRNFPELNSIAYSGESSGTNQDHIPIPSSYWSGTVAPETRAMITWLQSYPFVLSANMHDGDLVANYP YDTAKSGGFWGSGYAATPDDALWRDLASTYAQAHGTMATTGGGSCGFQGQGGITNGADWYSLSGGMQDFNYLHTNCY ELTLELGCDKYPRESELRMEWNNNKESLLAFMEKVHIGIKGVVTDTNGNGVADAKIKVQGNAHGVNSAADGDYWRLL RPGTYSVTATKGQASQTKSCTVGSGAATTCDFTLDAAGVLSGGTTGTGRLDISM
>tetraspanin
MEAKGGMQCIKYLLFAFNLIFWITGLAMIGVGATVKIAFGDYFDFTGNAFASAPVFLIILGVFVSIIGFLGC CGAIKENYCMVTTFAGRLSLIFILEIAAGITGYVLRNDIENVIDTAMTDFMMKWTNKTEGRLAFDGVHKKFECCGV TNYTDWFDKSPNFNPNNQPNVPGSCCLNAKTEFDVKNPDDTCGEGADTTKINTEGCLLKLEGWLAQNILIIGGVGI GIAFIQVVGIIFACCLMRSIKQEYEVV；
>legumain
MLSIVMLSLFGAVLGFPADFEEAIPVAEPEPGVNWAVLIAGSTGWGNYRHQADVCHAYQILHRNGIPDERIV VMMADDLAHNIRNPTKGIIINHPDGKDVYHGVPKDYTRFDVTAKNFLRVLKGDKEGVAGIGSGKVIESGPHDNVFV YYTDHGAPGIVAMPHGGMLHADDLVTTLKEMHQENKFNKLVFYLESCESGSMFDKMLPENINIFATTAANGKESSY ACYMDTKRKTYLGDLYSVNWMEDSDVEKVDKETLEQQFEKVKMLTNRSHVQEYGDVNMKNDVIGLYQSTINYPSTP LPRYKQVPHDAVPAPDVPLAILNLRLETAETEEERQTVLAELKEELNLRTTIAQTMKDIATMATDDADKAEDILVN HHRDITARACYKAAVNYFHDKCYDLSQYEHALRHMYTLVNLCEEQVPLDRVTAAMDSVC；
>saposin B
MKSVAVVLAVLLASQCLAVRPPSRRGPALVRAGGLSIGQVFKPSKVDLDLCKFCIDFSQQALNQLLNIILQV GVVGTCGDICQALADKTGSQALGVACNLLCDIEGVKEFVKIIENADLDPIYYCELLKQCTIKDDGDAKITSLDVSP KSGPQGTFMIDIEYMSKNGTGTGEMVVDIDTVDGIPLGQGFILEAQEAGSYGQKISLKAQPDPDCDPTQGPCEQWM PGVYNVNIAICNGECGSKHPHSQIYDQGMTNFTITG；
>subtilisin-like protease
MKSLRVLAVLAAVLGVAMATAPMLRLDSKTAIPNEYIVVLQKNLTNYAVGKHMNSVKTLLTGTNDTKILFEH NFRWFKAYSIRTNQKMIRHLAEQPEVRYIEANQVVKAYQAQCQDQLEATWGLVRTVERDLLLDGIYHYQGGDGEGV DAYIIDTGIYTEHSEFGGRAKWGVDTVDTPSPETDENGHGTHVAGTVMSDAWGLAKKATAIAVKVLSRSGSGSTAG VIEGVEWTGQQHSGNNKKSVANMSLGGGRSDAMNEAVKAVVEAGVVMVVAAGNEGWEACNVSPASEPTAITVGCSD NEDDFCFFSNYGTCMDIIAPGQDVTSAWIGGQFADNTISGTSMSAPHIAGIVAKYMSRQSSVPSPEEVKDFLQTTS TKDKINQIPSNSDTLNYLGFMDCGGGPDV；
>arylsulfatase B
MLRVPLLLLTGALVGLVSAQDGSQNATKPHILFIVADDLGWNDVGWHNPDVKTPVLDKLAHEGVILNQSYVN YVCTPSRTAFMTGYYPYHAGSQHLVFLPQQAQGIPYNFTFLPEKLKDLGYATHMVGKWHLGFCNWKYTPTYRGFDS FYGYYNADEDYYTHVVAGGLDLRDDKEVVNTKNGQYGTYFFTDRMVDIIEKHPADTPLFLYLPFQNVHEPLEVPER FENIYMNVQNENRRKLLGMVSALDEAVGNVTMAMKNAGLWDNTLVIFTTDNGGWIIASGNNYPLRGGKVTLWEGGT RGVAFVHGKMLQKTGYTNNEMIHAVDWFPTILAAAGGTADDAMDGVSQLDTIMSGQPSPRTEFVYNIDDVFPTKQG AIRIICTLCLRVGDYKLIEGVAGKPDGWIHPDNLTDSQLDSGDPTSGTWLFNLKDDPTEHHNLADSMPDKLKEMQA KLAEYRKSLVPAINPPPDPKSFPKNWGGAWSPGWC；
>endo-beta-1,4-glucanase
MDQDYERRLLRQQNGQNCFGSPVSSPISPMAGRESLGTSPVSSPSKDKYGDRFIPSRAGANWEIGFNSIQGM YEKTSGQARKAREANSDNGKDGLAYTCLLKNELLGAGIEDLKEQTEDRRGVLSPTTPEKRNLFRYHLTAKQASPEN TDHLSPYSLSPVGKKSQKLLRSPRKQTRKISKIPFKVLDAPELQDDFYLNLVDWSATNILSVGLGTCVYLWSACTS QVTRLCDLSCDGDSVTSVNWNERGNLVAVGTHKGYVQVWDAMAGKRISMLEGHSARVGALAWNADILSSGSRDRLI LQRDVRTPSVVPERRLAGHRQEVCGLKWSPDHQHLASGGNDNKRIVYKKGTFVDSFRPTNMRCTALILLALLGAAR AQSYYEVTGDWGDRFQANVYIPVSQAVNGWTATLKFDKAVELEIWLAEVTSTSDGNTVFELKDMGWNANVPAGTFE LSFIANTQGSAANLVGLWFNGQEVTSGGTGTGTGTGTGTGTTHRPSTVSTSHTHATAPTAAQSGTTLGVVPPVVGA YDYVDALAKSILFYEAQRAGNLPGNNRVPWRRSCCQGDGQDVGLDLSGGWFDAGDHLKLHFPLSYTITVLSWGMIE FKRAYEAAGEMANALDSIKWVTDYLIKCHPSKFEFVAQVSTHGYSQNQILVWKYPSLVQVAKLTGHSYRVLYLAMS PDGEAIVTGAGDETLRFWNVFSKTRSNKESKSVLNLYTHIR；
>methionine adenosyltransferase
MANQDTFLFTSESVGEGHPDKLCDQISDAILDAHLKKDPNAKVACEACTKTGYIMVFGEVNTTATIDYQTVV RDVIKRVGYDHSDKGFDYNTCAVIQAVEQQSPDIAQGVHEKRGAEDIGAGDQGLMFGYASDETEECMPLTCMLAHG LNRKLAELRRDGTFTWLRPDTKTQVTVEYRMSGGACVPIRVHTVVMSVQTDDVVTLDDLREQLKERVVKAVIPSRY LDDKTIYHLQPSGKFQRGGPQADAGLTGRKIIVDTYGGWGAHGGGAFSGKDFSKVDRSAGYAARWVAKSLVKAGLC RRVLVQVSYAIGISHPLSVLVNSYGTCQDGMTDARLLKIVNKNFDLRPGVIVRELGLQRPIYEKTACYGHFGRMDP DFTWEQPKKLDLNV；
>pancreatic lipase-like protein
MLLSVLSILSLACAAQAAEVCYGDLGCFTNIAPFWGLNRPLSSLPDPPDVVGTKFYLYTRANPTMALRERLV ADSIATLSASHFSSSKPTRMVIHGFTGSAEHAWVQTIVDELLLKEDINVITVDWADGASIGGSYGQATANSRVVGA EVAKIVNYMSAQTGANARNFHLIGHSLGCHVAGYAGDILGNVGRITALDASEPYFDGMDAIVKLDPTDALFVDVIH SDGSPFIGTLGMGTSLPTGHVDFYPNGGEYQPGCHDNFVSSVVSTGFGLLTEGYDGAEAAAACSHLRAIDYFTESI NSECPFTAYPCESYDKFKDGFCMSCAGSTCSQMGYRARDHYGARGKLYLTTTDGTQPGGKFCAYHYKVQVTSSTRM EETAGRIYVTFQGPSGITDTMEVTDGDVMDLKPGVTYQKLVSSASNIGDVSRVGLRFERSSSIWDFFAAQYYTVGK VTITAGETQTSYKFCGSDTPVNNGETLQLTQVGGTC；
>plasminogen
MRTLLALLGLLALTAAQDCYVGRGSAYRGAVNVTSDGLECQPWSVQAPHGHAFTPANYPDAGLDGNYCRNPD NDLRPWCYTMDPNVRFGYCDAPQCDCLFNTGEFYKGSKATSISGLTCQRWDSQSPHQHDRTPQNYPEHDLRENYCR NPDGEPRTWCYTTDPNVRWEHCDVPKCDTADLGTCGVPAIQPTFSRIVGGSVATPGSWPWQVSLGYGSNGQHVCGA TLIAPEWVLSAAHCFAQLSTNPGSFIVKVGKHNKASTDSTEQRMQVAQIIVHPRYQQDGQNTNDIALLKLTQRVQL NDYVSQACITETEAPEGAICVATGFGNTEGTGGDNYLKQVQLPSLSNAQCRSWLGSVIKSTMVCAGYEGGGSDTCQ GDSGGPLTCPRLGQWFVSGVTSFGQGCADPRKPGVYTRVGYYIDWILKYTADN；
>peroxiredoxin V
MLIPVTICSLARRLPSAIGARCAIYTATAYNMPIKVGDKLPGIDLYENTPGNKVNVSELFAGKKGVLFAVPG AFTPGCSKTHLPGYVGKAGDLKAKGVQVIACVSVNDPFVMEAWGKDQKAEGKVRMLADTGAEFTKAIGLDLDATGL LGNIRSKRYSMLVEDGEVKQLNVEPDGTGLTCSLAEGLKL；
>VCBP4
MAPFTPFVLCIILAGAMAQSPFRIMTVTVPRPEVTANVGSDVKLPCFYSIPAASSGLNPTINWYKGDEGIAS ATKIFSLSYHGSTQIAEAYEGYELRVELESLTDPTLKLSGVRASDHGRYWCAVFDSDEHNQFGMDSKSVLLTVIDP SIQQDQGMGRPGRVELRVPAVRQVYQGEDVELTCECDDCHWTSTKSWFTVSFDDTWAATEELVATVGPCVSHQCHG GTTTVAPAFRDHFSATREALRISAAKRLDKRRYWCQVEDMTFSRGSGVDAQSVALAVESRCEGKPAGRYQHPDDCS KYYTCAEGGLQYDGISACPPGLMYDQANGYCNWATQVTCL；
>VCBP1
MKFVLGLVLLAVGAHAMTIVTVSTPEPKVEASVGGSAELKCEFDIQPNSTQPPTIAWFKGNDDFRGVERIYT GHKVWGNETERREDSFGDYIGRVEVADLDKPAIKISGIKSTDFARYWCTVAEWGVRTELGVDAKSVLLTETGHSEA SIDISVSGEKDVEEGGDVEMTCRCHGCTSAAIFDWFKGAFAGSEWVTTGNYTHIAAKVDVGVLGFPNPIEIDDGFG QFSVTPSNSLRLTGAQVADAGRYWCKVTSGGSVDIKATVLKVKVPEFTCAGKADGYYPDPEDCAMYYQCLYGFPQP FHRPCGYAGMVFNPEHLYCDWAFNVGPPCGSKA；
>VCBP3
MQMFLLVSVCLGMAYGQSIMTVRTTHTEVEVHAGGTVELPCSYQLANDTQPPVISWLKGASPDRSTKVFKGN YNWQGEGLGFVESDSYKESFGDFLGRASVANLAAPTLRLTHVHPQDGGRYWCQVAQWSIRTEFGLDAKSVVLKVTG HTPSNNVHVSTAEVVQVDEGNDITMTCPCTDCANANVTWYTGPTFFENYETGTYQPLANKNQFGITWFSSEIAGRA SFSGARNLVIRAAKITDAGRVWCELATGQGELDADRSSTILKVQLEPFTCDGKPTGLYADPTACDYYYQCIPGYPP LHRPCGYAGMVFNEEMQYCDWDINVPPPCGSKPV；
>VCBP5
MSVLLVSIILLLYVGPESADAVSITTVTVPDRGGWVVMWPRPGDPTWVNRIEFRCEYSISPASATPPTITWL KGVFADADRQVIYKWSSSGEVYVHPEYAGRVSVPSRTHPTLVLTDAKFDDWGRYWCRVTNEDQSDEFGTDEESLLF WYKLGYDPPTRLSQVSLDKTPVRVATGGTARLDCTGTSGREASILWVKGPTSCTQGESCDSYETVIHKSAVWGAGN PEPENITISPSFDGRVSLDADGSGSFTPTLTITDIRPSDSGRYWCAPDISEVYSNLGLLNRDAQSVVIIVNDPGTE PTCAGKPDGMYQHPADCAQFYTCSGGLSYGTNNCPAGLVFNQELQLCDWANNVICL；
>Gram-negative bacteria-binding protein
MALLAVLLFHAALWAAGEAQGTYLGCYIDTPNDRDFPHVTTMTYTNGYTTCSQYCASLGYSYIGLQYAIECF CGHTYGKHGRAAESDCYYGCSGGTGGKCGGDYRNTVWDIGDVGGGSTGTGTSDNLELVFEDTFDTLDTTKWRPEVT AGGGGNEEFQYYMGHPDNLYVQNGNLYLKPTLASEHYGDNFMTSGYIDFWHGYNYVGCFNDGGDADLTESLLTASD MNPGKCVTHCANGGYEYFGLKNTECRCGNSFGRFGHGHGCHISCPGDSGKECGGNGVTSVYGQTYGDCTSDFPQGR ACYLQGSQNAPLPPIQSARVTTVESFAFKYGKVEVWAKLPTGDWIWPAIWLLPRDNVYGGWPASGEIDIMESRGNR NLYGSWGGSIGVDDMGSTLHWGPRWPYNGFQQTTASKVEVDDQEVLLVSPPTNFWDMGNFGLPSYENPWAGGGKMT PFDREFYLIFNVAVGGTNYYFPDDATNQPYPKPWSNNNSREGAMRDFWNAKNQWYPTWNGDDVAMQIQSIKVWRFV；
>alpha2-macroglobulin
MRGLLLLCGVLGSVPAQKLDPTPGPTPGTPGTFLIMAPKVVRPGRPLTLSVSILDPAAASVQVTATLLQNGR PTGTPVSGDFSRNADGTLDLVVPGALTAGSAYKLKVEGTGGLTFTEEKDLTFNSKSSSIFVQTDKGKYKPGQTVKM RALAVDAELKTKKEPFDIDIFDPQGNEIVQWRGLQNDDGLVEKEFALSDQPLLGDWKIVAMLPGGDEVEQPFGVEE YVLPKFEVTISTNSFFARTEDTLQGTFNAKYTYGKNVEGQGKVTVKLRSVGSNFRPTDNSIEHNNIAMVEGSGSFQ FTRGDIEGLYGNPDQLYYGSYQLELIVEVTEGLTGNTQEATKELDLVQTKYKLESFNTPDNFKPGLQYTAYVRTSY HDGTPMLGMDLAQPVTLSKNYGWDGPTAPPAEELTIPGSGVIKIVMDAPSTANSMTLSVMYNDISETATTHTAQKA ESPSSSYIQVTTSTPEVTAGEDAQFTVKSTSPIGYFSYQVLSRGNIVVVDRVEVTPEDTLKSFTVPTNTQMAPSSR MVVYWMTDTGEICNDGLTFTVKGAFENQVSLSYDKDRAEPAEDIAVTVRADPDSLVALLTVDQGVLLLQSGNDITQ DKVLAELEEYDPAEDTSNSGGPEIMWRRRKRSIWWPMPTSGDDAHSVFENAGVIVLTDAMVYQKQPDYWWQWGILP VADFDLGMMEFMPAAAAGESAGGGGGRGGLQEVKRVRSFFPETWLWTNATAGADGTAVFASTVPDTITSWVASAFA LNDVSGLGVADTPASMEAFRPFFVSLNLPYSVVNKEETAIQALVFNYMDVDMDVTVTLKGSSGFMHIVMDTNDVNS IGTEVAGDQAVVLTVPAGSSRSKSFPIIPKQFGAITVKVLAQSTLAADAVERQLLVEPEGEQKEYTKSFILDLGDS GNGDITKDLYLSVPQSGLVAGSAYARVSVMGDMMGSTISGLDSLLQMPYGCGEQTMITFAPNVYVMHYLNETNQVT PEIKSKALKFMTSGYQRELTYTHDDGSFSAFGSSDASGSTWLSAFVMKSFSQAMDYIYIDPKVLAKTIRWLIRQQQ DTGVFSEPGNVIHKEMQGGLSSDVTMTSYVLVALMEYDKILATLDAPDSDKVDVTIAKNRAVAFLESRLDSETDVY TIVTMTYALTLAGSRRKDAAFQKMNSLATTEAGQMYWTKGESATSPPVSDDLMWTPPYHNPPSADVEMTAYALLTY LDRDDLVAGMPIMKWLTEQRNAYGGYSSTQDTVVGLQALGYFAAAVRAGGLDMTVTITSDTDTDFSHTFTVNDQNA IVLQRVEIPNVSGTLTARAQGQGMGLLQLFVRYNVDVVEAVPAFNLIATVKNSDTNSVTCETCGTYQLGGSSGMAV MEIGIPSGFYVEDADLMALVDNQAMPTLKRAERSTDNKRAVIYFDEMNASPTCVTLTSHRSMPVAKTQPSVVKVYD YYKPDVKASNIYQSETLKNTNICEVCADCTGCSGRQGRPRSAAAGLAVDTLLGVVLLLLTRLLVRQCRPTNTCEEC LNVPCSELVTKLRL；
>chitotriosidase 1-like protein
MLTVLALLCVMLSLGQASVIPAQRNTYRRVCYHTNWSQYRHGVGQFFPEDIDPTLCTHAIYAFAKMTNNQLQ PYEWNDDSTDWSTGMYERFNTHLKPHGVKTLLGVGGWNFGSTAFSDMAATAAGRRTFVTTSIDFLRQRNFDGLDLD WEYPAARGGRPSDKQTFTLLRKELREAFDAEGARTGRAPLLLTAAMPAGEDNAMNGYEMAEVAGYLDFLNLMAYDL HGQWETYTGLNSPLYAAGDETGDDRKLNQAFAVDMWLAGGVPPSKLNLGMGTYGRSFTTTGDNSIRAPANGGGNAG LYTREKGYLAYYEICTMLNQGATRVFHSEHLSPYAYLGNQWVGYDDVESLTYKVEYLKSKNLGGAMVWAYDIDDFS GSSCGQGRYPLMNAIKNLLETGSAGVVLPPGPTHPPINPATQAPNPGVTQGPNPVVTQAPVVVEQGNLAAVTCDSK PDGFHPDPTDCGKYFQCWGGTMWPGHCSNGLQWNQAMLGCDWPYNVNC；
>big defensin
MEKKTAYCLLFLVLLVPYTALGAVLKRAPAKKEKRAVPLAVPLVYWGASVSPAVWNWLLVTFGAAAVAAAAV TVSDNDSHSCANNRGWCRSRCFSHEYIDSWHSDVCGSYDCCRPRY；
>proprotein convertase subtilisin/kexin type 1
MGRFYAWLVLAIVAFSSHGRAEDGEDQPGHFTNTWAVEIYGGPDRADLLALDHGYENLGQIGNLEDHYLFRH KDVPHRSRRGAHQHTKRLGDDERIQWVAQQVGRARSKRGPMGQQRRQSDDTRPMTFRDPYWEKQWYLHDTRTSTNL PKLDLHVLPVWRKGITGKGIVVAVLDDGIEKDHPDLVDNYDPDASYDFNDNDDDPQPRYEETNENKHGTRCAGEIA MAANNSECGVGIAFNARIGGVRMLDGVVTDAVEASSIGFNIQHVDIYSASWGPNDDGKTVEGPEKLARAAFEKGVR EGRGGKGSIFAWASGNGGSNGDNCDCDGYTSSIYTVSISSASQQGGSPWYGEKCASTLATAYSSGEYKDQKISSTD LHHECTDSHTGTSAAAPLAAGVLALALEANPNLTWRDVQHLIVWTSEYDPLSSNPGWFQNGAGLWVNSRFGYGLLN AEAMVDMALTWKTVPEKTKCEVRIENFQPRDLGNGEEIIIELETDGCRGQNHVEALEHVQVKTTIDYSRRGDLRIV LTSPSGTSTTLLDTRRQDKSQMGFQDWPFMSTHNWGEKPQGKWTLTIEDKSDHAENNGVVKDVVLILHGTPEQPAY QSGGRVYTDYNRVQGDRNVEISAAKAAPAPAADAAPARGSEERIPGSGPFGSAASVVIEEIPEKETEFLVNWQDGM NRYETDFNPVNSGPFEPSADAGSDVYMDAEDLRPWEAFKYLQRQMKSPSGQRHPAHAYNKPSSQYIRDPWVSQNAL DKEANLVKYYLQLLGYES；
>Toll-interacting protein
MATKVTKRGQVMLGDLPDDFLRIEATPQQLQATADMQAAQMYAQYGFMGQSSGRLSITVAQAKLAKNYGVTR MDPYCRIRVGHAVFETPTAYNGAKNPRWNKVVHAPIPQGVNSFYLEIFDERSFTMDDRVAWGHITIPESVFNGETV DDWYTLSGRQGDDKEGMINLVLSHSTVPMMQQVPQVMPQMPMMMVPQPVYYPTVQAGVPYQVPAGQMPAQQQPPAQ PAITEEDLKALQDMFPNMDAEVVRSVLEANRGNKDAAVNSLLSITAE。
3. the preparation method of the biological agent for algae of degrading described in claim 1, it is characterised in that the following steps are included: (1) is obtained Take the coecum tissue of lancelet；(2) the full length mRNA s of coecum tissue is extracted, and is converted into cDNA；(3) homologous recombination side is utilized Method or total gene synthesis method construct overall length functional protein group library；(4) pass through vivoexpression functional protein group product.
4. specific expressed protein answering in the biological agent of preparation degradation algae in the epithelial cell of lancelet coecum With.
5. application according to claim 4, it is characterised in that the protein and the mass ratio of algae are 1:100-1: 100,000,000。
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Application publication date: 20190614