CN109876782A - A kind of aqueous phase preparation method of kitasamycin tartrate molecular imprinted polymer on surface and its application - Google Patents
A kind of aqueous phase preparation method of kitasamycin tartrate molecular imprinted polymer on surface and its application Download PDFInfo
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- CN109876782A CN109876782A CN201910065208.4A CN201910065208A CN109876782A CN 109876782 A CN109876782 A CN 109876782A CN 201910065208 A CN201910065208 A CN 201910065208A CN 109876782 A CN109876782 A CN 109876782A
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Abstract
The invention discloses a kind of aqueous phase preparation method of kitasamycin tartrate molecular imprinted polymer on surface and its applications, belong to analytical chemistry and pollutant analysis detection technique field.Technical solution of the present invention main points are as follows: with pure water be prepare solvent, using [VAIM] Cl and/or AMPS as function monomer, kitasamycin tartrate is template molecule, pass through crosslinking agent N, the collective effect of N '-methylene-bisacrylamide and initiator azodiisobutyronitrile prepares kitasamycin tartrate molecular imprinted polymer on surface in styrene-divinylbenzene carrier particle surface.What the present invention used prepares solvent green non-pollution, molecularly imprinted polymer uniform particle sizes obtained, adsorption capacity are big, active site with three-dimensional cavity and specific bond with template molecule structure matching, it can be used in the Selective Separation of trace kitasamycin tartrate, enrichment and detection and analysis in environmental sample, have broad application prospects.
Description
Technical field
The invention belongs to analytical chemistry and pollutant analysis detection technique fields, are mainly used for macrolide in environmental sample
Selective Separation, enrichment and the analysis detection of class antibiotic, and in particular to a kind of kitasamycin tartrate surface molecule print is poly-
Close aqueous phase preparation method and its application of object.
Background technique
Macrolide antibiotics is the general name with the antibiotic of big lactone ring skeleton structure, by inhibiting in bacterium
The synthesis of protein in physianthropy and is veterinarily widely used to resist gram-positive bacteria and negative bacterium.Wherein,
Kitasamycin is largely used in animal husbandry at present based on its excellent anti-microbial property, with constantly making for kitasamycin
With Environmental Residues also gradually attract people's attention.It is well known that minimal residue of the antibiotic in complex environment medium is just
Greatly harm can be generated to the ecosystem and human health.Therefore, to the analysis detection of trace kitasamycin in environmental sample
It becomes as researcher's focus of attention.
Molecular imprinting technology is also known as molecular imprinting technique, is a kind of functional polymer technology of preparing based on molecular recognition.
Due to the presence in complementary steric structure and specific activity site, molecularly imprinted polymer has " memory function " to object, energy
Object in enough Selective recognition complex matrices realizes that complex matrices interfere lower low concentration intentional object in actual environment sample
Selective Separation and enrichment.However, current molecularly imprinted polymer toxic has in the nonpolarity such as acetonitrile, chloroform, toluene mostly
It is prepared in solvent, this aspect can bring secondary pollution, the polar solvent being on the other hand widely present in pollutant to environment
In the quasi polymer often show poor absorption property, therefore, how in green polarizable medium water to realize molecular engram
The preparation of polymer just becomes the focus of people's research hot spot.
The present invention uses surface molecule print technology, is to prepare solvent, 1- allyl -3- vinyl imidazole villaumite with pure water
([VAIM] Cl) and/or 2- acrylamide-2-methyl propane sulfonic (AMPS) are function monomer, and kitasamycin tartrate is template point
Son, by crosslinking agent N, the collective effect of N '-methylene-bisacrylamide (MBA) and initiator azodiisobutyronitrile (AIBN),
Molecular imprinted polymeric adsorbent material is prepared in styrene-divinylbenzene carrier surface.Prepare the table in this approach at present
Face molecularly imprinted polymer material is there is not yet pertinent literature is reported.
Summary of the invention
Kitasamycin tartrate surface molecular is prepared in green medium aqueous solution the object of the present invention is to provide a kind of
The method of imprinted polymer, kitasamycin tartrate molecular imprinted polymer on surface made from this method is to object and its structure
Analog has preferable selective absorption performance.
The present invention adopts the following technical scheme that a kind of kitasamycin tartrate surface molecule print is poly- to achieve the above object
Close the aqueous phase preparation method of object, it is characterised in that detailed process are as follows: by function monomer [VAIM] Cl and/or AMPS and template molecule
Kitasamycin tartrate is dissolved in the pure water solvent added with styrene-divinylbenzene carrier granular, after 30 DEG C of prepolymerizations
Crosslinking agent N, N '-methylene-bisacrylamide and initiator azodiisobutyronitrile is added, passes through crosslinking agent N under nitrogen protection,
The collective effect of N '-methylene-bisacrylamide and initiator azodiisobutyronitrile is in 60 DEG C of generation polymerization reactions in styrene-
Divinylbenzene carrier particle surface coats kitasamycin tartrate molecular imprinted polymer on surface layer, the kitasamycin tartrate
Molecular imprinted polymer on surface being capable of macrolide antibiotics pollutant in Selective recognition environmental water sample.
Preferably, the mass ratio that feeds intake of the template molecule and carrier granular is 1.1-1.4:1.
Preferably, the molar ratio of function monomer [VAIM] Cl and AMPS is 0-3:3-0.
Preferably, the molar ratio of the template molecule, function monomer, crosslinking agent and initiator is 1:4-8:15-25:
0.24。
The aqueous phase preparation method of kitasamycin tartrate molecular imprinted polymer on surface of the present invention, it is characterised in that
Specific steps are as follows: it is molten that template molecule kitasamycin tartrate and function monomer [VAIM] Cl and AMPS are dissolved in 25 mL pure water
In agent, 0.15 g styrene-divinylbenzene carrier granular is added, wherein the mass ratio of template molecule and carrier granular is
1.4:1, sealing, after vibrating 4 h of prepolymerization in 30 DEG C of water-baths, with template molecule, function monomer, crosslinking agent and initiator mole
Crosslinking agent MBA and initiator A IBN is added than the proportion for 1:4:20:0.24, after ultrasound, logical nitrogen, sealing in 60 DEG C of water-baths
Oscillation 20 h of polymerization, products therefrom is rinsed with methanol dry after be 8:2 with volume ratio the mixed liquor Soxhlet of methanol and acetic acid mention
Kitasamycin tartrate molecular imprinted polymer on surface is obtained in 60 DEG C of vacuum drying after taking 40 h, again with methanol to rinse, by 10
Kitasamycin tartrate molecular imprinted polymer on surface made from mg and non-imprinted polymer and 10 mL molar concentrations are 0.2
mmol L-1Kitasamycin tartrate aqueous solution mixing, shaken at room temperature adsorb 3 h after, take supernatant with ultraviolet specrophotometer
The absorbance value under 232 nm is detected, is computed, the kitasamycin tartrate molecular imprinted polymer on surface is to wine in aqueous solution
The adsorbance of stone acid kitasamycin is 80.87 μm of ol g-1。
Kitasamycin tartrate molecular imprinted polymer on surface of the present invention is in Selective recognition environmental water sample
The application of macrolide antibiotics pollutant, wherein kitasamycin tartrate molecular imprinted polymer on surface uniform particle sizes, have
There is the action site in the 3 D stereo hole and specific bond that match with kitasamycin tartrate, can be used in Complex Water Environment
The Selective Separation and enrichment of micro macrolide antibiotics pollutant in sample, the kitasamycin tartrate surface after regeneration
Molecularly imprinted polymer repetitive cycling uses.
Compared with the prior art, the invention has the following beneficial effects:
1, the present invention provides a kind of aqueous phase preparation method of kitasamycin tartrate molecular imprinted polymer on surface, party's legal systems
Kitasamycin tartrate molecular imprinted polymer on surface there are the excellent performances such as uniform particle sizes, adsorption capacity be big.
2, the present invention use pure water to prepare solvent, compared with previous acetonitrile, chloroform etc. nonpolarity toxic organic solvents, one
Aspect greatly reduces pollution of the solvent to environment, on the other hand improves what imprinted polymer was widely present in trace contaminant
Adsorption capacity in polarizable medium is laid a good foundation for synthesis of the molecularly imprinted polymer in water phase isopolarity medium.
Specific embodiment
Above content of the invention is described in further details by the following examples, but this should not be interpreted as to this
The range for inventing above-mentioned theme is only limitted to embodiment below, and all technologies realized based on above content of the present invention belong to this hair
Bright range.
Embodiment 1
By template molecule kitasamycin tartrate, function monomer [VAIM] Cl and AMPS(n[VAIM]Cl:nAMPS=1:1) it is dissolved in 25
In mL pure water solvent, 0.15 g styrene-divinylbenzene carrier granular is added, wherein the matter of template molecule and carrier granular
Amount is than being 1.4:1, sealing, after vibrating 4 h of prepolymerization in 30 DEG C of water-baths, with template molecule, function monomer, crosslinking agent and initiation
Crosslinking agent MBA and initiator A IBN is added in the proportion that agent molar ratio is 1:4:20:0.24, in 60 DEG C after ultrasound, logical nitrogen, sealing
In water-bath oscillation polymerization 20 h, products therefrom with methanol rinse dry after be 8:2 with volume ratio methanol and acetic acid mixed liquor
40 h of Soxhlet extraction, again with methanol obtain kitasamycin tartrate surface molecule print in 60 DEG C of vacuum drying after rinsing and polymerize
Object, kitasamycin tartrate molecular imprinted polymer on surface uniform particle sizes, has good selectivity absorption to template molecule
Performance.
Embodiment 2
By template molecule kitasamycin tartrate, function monomer [VAIM] Cl and AMPS(n[VAIM]Cl:nAMPS=1:1) it is dissolved in 25
In mL pure water solvent, 0.2 g styrene-divinylbenzene carrier granular is added, wherein the matter of template molecule and carrier granular
Amount is than being 1.1:1, sealing, after vibrating 6 h of prepolymerization in 30 DEG C of water-baths, with template molecule, function monomer, crosslinking agent and initiation
Crosslinking agent MBA and initiator A IBN is added in the proportion that agent molar ratio is 1:4:20:0.24, in 60 DEG C after ultrasound, logical nitrogen, sealing
In water-bath vibrate 0 h of polyase 13, products therefrom with methanol rinse dry after be 8:2 with volume ratio methanol and acetic acid mixed liquor
60 h of Soxhlet extraction, again with methanol obtain kitasamycin tartrate surface molecule print in 60 DEG C of vacuum drying after rinsing and polymerize
Object, kitasamycin tartrate molecular imprinted polymer on surface uniform particle sizes, has good selectivity absorption to template molecule
Performance.
Embodiment 3
By template molecule kitasamycin tartrate, function monomer [VAIM] Cl and AMPS(n[VAIM]Cl:nAMPS=0:3) it is dissolved in 25
In mL pure water solvent, 0.15 g styrene-divinylbenzene particle is added, wherein the mass ratio of template molecule and carrier granular
For 1.4:1, sealing, after prepolymerization 4h is vibrated in 30 DEG C of water-baths, with template molecule, function monomer, crosslinking agent and initiator mole
Crosslinking agent MBA and initiator A IBN is added than the proportion for 1:4:20:0.24, after ultrasound, logical nitrogen, sealing in 60 DEG C of water-baths
Oscillation 24 h of polymerization, methanol rinses dry after be 8:2 with volume ratio methanol and acetic acid 48 h of mixed liquor Soxhlet extraction, then use
Kitasamycin tartrate molecular imprinted polymer on surface is obtained in 60 DEG C of vacuum drying after methanol flushing, the kitasamycin tartrate
Molecular imprinted polymer on surface uniform particle sizes have good selectivity absorption property to template molecule.
Embodiment 4
By template molecule kitasamycin tartrate, function monomer [VAIM] Cl and AMPS(n[VAIM]Cl:nAMPS=1:1) it is dissolved in 25
In mL pure water solvent, 0.15 g styrene-divinylbenzene particle is added, wherein the mass ratio of template molecule and carrier granular
For 1.4:1, sealing is rubbed after vibrating prepolymerization 4h in 30 DEG C of water-baths with template molecule, function monomer, crosslinking agent and initiator
Crosslinking agent MBA and initiator A IBN is added than the proportion for being 1:4:20:0.24 in you, in 60 DEG C of water-baths after ultrasound, logical nitrogen, sealing
Middle oscillation polymerize 24 h, products therefrom is rinsed with methanol dry after be 8:2 with volume ratio methanol and acetic acid mixed liquor Soxhlet
48 h are extracted, obtain kitasamycin tartrate molecular imprinted polymer on surface in 60 DEG C of vacuum drying after again with methanol flushing, it should
Kitasamycin tartrate molecular imprinted polymer on surface uniform particle sizes, have good selectivity absorption property to template molecule.
Embodiment 5
By template molecule kitasamycin tartrate, function monomer [VAIM] Cl and AMPS(n[VAIM]Cl:nAMPS=3:0) it is dissolved in 25
In mL pure water solvent, 0.15 g styrene-divinylbenzene particle is added, wherein the mass ratio of template molecule and carrier granular
For 1.4:1, sealing is rubbed after vibrating 4 h of prepolymerization in 30 DEG C of water-baths with template molecule, function monomer, crosslinking agent and initiator
Crosslinking agent MBA and initiator A IBN is added than the proportion for being 1:4:20:0.24 in you, in 60 DEG C of water-baths after ultrasound, logical nitrogen, sealing
Middle oscillation polymerize 24 h, products therefrom is rinsed with methanol dry after be 8:2 with volume ratio methanol and acetic acid mixed liquor Soxhlet
48 h are extracted, obtain kitasamycin tartrate molecular imprinted polymer on surface in 60 DEG C of vacuum drying after again with methanol flushing, it should
Kitasamycin tartrate molecular imprinted polymer on surface uniform particle sizes, have good selectivity absorption property to template molecule.
Embodiment 6
By template molecule kitasamycin tartrate, function monomer [VAIM] Cl and AMPS(n[VAIM]Cl:nAMPS=1:1) it is dissolved in 25
In mL pure water solvent, 0.15 g styrene-divinylbenzene particle is added, wherein the mass ratio of template molecule and carrier granular
For 1.4:1, sealing is rubbed after vibrating 4 h of prepolymerization in 30 DEG C of water-baths with template molecule, function monomer, crosslinking agent and initiator
Crosslinking agent MBA and initiator A IBN is added than the proportion for being 1:6:20:0.24 in you, in 60 DEG C of water-baths after ultrasound, logical nitrogen, sealing
Middle oscillation polymerize 24 h, products therefrom is rinsed with methanol dry after be 8:2 with volume ratio methanol and acetic acid mixed liquor Soxhlet
48 h are extracted, obtain kitasamycin tartrate molecular imprinted polymer on surface in 60 DEG C of vacuum drying after again with methanol flushing, it should
Kitasamycin tartrate molecular imprinted polymer on surface uniform particle sizes, have good selectivity absorption property to template molecule.
Embodiment 7
By template molecule kitasamycin tartrate, function monomer [VAIM] Cl and AMPS(n[VAIM]Cl:nAMPS=1:1) it is dissolved in 25
In mL pure water solvent, 0.15 g styrene-divinylbenzene particle is added, wherein the mass ratio of template molecule and carrier granular
For 1.4:1, sealing is rubbed after vibrating 4 h of prepolymerization in 30 DEG C of water-baths with template molecule, function monomer, crosslinking agent and initiator
Crosslinking agent MBA and initiator A IBN is added than the proportion for being 1:8:20:0.24 in you, in 60 DEG C of water-baths after ultrasound, logical nitrogen, sealing
Middle oscillation polymerize 24 h, products therefrom is rinsed with methanol dry after be 8:2 with volume ratio methanol and acetic acid mixed liquor Soxhlet
48 h are extracted, obtain kitasamycin tartrate molecular imprinted polymer on surface in 60 DEG C of vacuum drying after again with methanol flushing, it should
Kitasamycin tartrate molecular imprinted polymer on surface uniform particle sizes, have good selectivity absorption property to template molecule.
Embodiment 8
By template molecule kitasamycin tartrate, function monomer [VAIM] Cl and AMPS(n[VAIM]Cl:nAMPS=1:1) it is dissolved in 25
In mL pure water solvent, 0.15 g styrene-divinylbenzene particle is added, wherein the mass ratio of template molecule and carrier granular
For 1.4:1, sealing is rubbed after vibrating 4 h of prepolymerization in 30 DEG C of water-baths with template molecule, function monomer, crosslinking agent and initiator
Crosslinking agent MBA and initiator A IBN is added than the proportion for being 1:4:15:0.24 in you, in 60 DEG C of water-baths after ultrasound, logical nitrogen, sealing
Middle oscillation polymerize 24 h, products therefrom is rinsed with methanol dry after be 8:2 with volume ratio methanol and acetic acid mixed liquor Soxhlet
48 h are extracted, obtain kitasamycin tartrate molecular imprinted polymer on surface in 60 DEG C of vacuum drying after again with methanol flushing, it should
Kitasamycin tartrate molecular imprinted polymer on surface uniform particle sizes, have good selectivity absorption property to template molecule.
Embodiment 9
By template molecule kitasamycin tartrate, function monomer [VAIM] Cl and AMPS(n[VAIM]Cl:nAMPS=1:1) it is dissolved in 25
In mL pure water solvent, 0.15 g styrene-divinylbenzene particle is added, wherein the mass ratio of template molecule and carrier granular
For 1.4:1, sealing is rubbed after vibrating 4 h of prepolymerization in 30 DEG C of water-baths with template molecule, function monomer, crosslinking agent and initiator
Crosslinking agent MBA and initiator A IBN is added than the proportion for being 1:4:25:0.24 in you, in 60 DEG C of water-baths after ultrasound, logical nitrogen, sealing
Middle oscillation polymerize 24 h, products therefrom is rinsed with methanol dry after be 8:2 with volume ratio methanol and acetic acid mixed liquor Soxhlet
48 h are extracted, obtain kitasamycin tartrate molecular imprinted polymer on surface in 60 DEG C of vacuum drying after again with methanol flushing, it should
Kitasamycin tartrate molecular imprinted polymer on surface uniform particle sizes, have good selectivity absorption property to template molecule.
Embodiment 10
Respectively by kitasamycin tartrate molecular imprinted polymer on surface made from 10 mg embodiments 1 and non-imprinted polymer with
10 mL molar concentrations are 0.2 mmol L-1Kitasamycin tartrate aqueous solution mixing, shaken at room temperature adsorb 3 h after, take supernatant
Liquid is with the absorbance value under 232 nm of UV spectrophotometer measuring.It is computed, kitasamycin tartrate surface molecule print is poly-
Closing object is 80.87 μm of ol g to the adsorbance of aqueous solution mesotartaric acid kitasamycin-1, non-imprinted polymer pair under corresponding conditions
The adsorbance of kitasamycin tartrate is only 14.57 μm of ol g-1, imprinted polymer is relative to non-imprinted polymer to template point
The selectivity factor of son is 5.55, and it is preferable to show that the kitasamycin tartrate molecular imprinted polymer on surface has template molecule
Specific adsorption performance.
Embodiment 11
By kitasamycin tartrate molecular imprinted polymer on surface made from 10 mg embodiments 1, non-imprinted polymer respectively with 10
ML molar concentration is 0.2 mmol L-1Kitasamycin tartrate, Tylosin Tartrate, tilmicosin phosphate aqueous solution it is mixed
It closes, after shaken at room temperature adsorbs 3 h, takes supernatant with the absorbance value under UV spectrophotometer measuring maximum absorption wavelength.Through
It calculates, which is respectively 80.87 μm of ol g to the adsorbance of above-mentioned substrate-1、
49.587 μmol g-1、45.21 μmol g-1, under corresponding conditions, non-imprinted polymer is below the adsorbance of above-mentioned substrate
4.76 μmol g-1, show kitasamycin tartrate molecular imprinted polymer on surface obtained to the analogue of template molecule
Also there is preferable adsorption capacity.
Embodiment 12
By kitasamycin tartrate molecular imprinted polymer on surface made from 10 mg embodiments 1, non-imprinted polymer respectively with 10
ML molar concentration is 0.2 mmol L-1Kitasamycin tartrate, Ceftriaxone Sodium, Amoxicillin Sodium, chlorination-N- butyl-pyridinium
Aqueous solution mixing takes supernatant with the extinction under UV spectrophotometer measuring maximum absorption wavelength after shaken at room temperature adsorbs 3 h
Angle value.It is computed, the absorption of the kitasamycin tartrate molecular imprinted polymer on surface to template molecule kitasamycin tartrate
Amount is 80.87 μm of ol g-1, the adsorbance of non-imprinted polymer paratartaric acid kitasamycin is lower than 2 μm of ol g-1；And corresponding item
The molecular imprinted polymer on surface and non-imprinted polymer are to non-structural analog Ceftriaxone Sodium, Amoxicillin Sodium, chlorine under part
The adsorbance of change-N- butyl-pyridinium is below 5.48 μm of ol g-1, show kitasamycin tartrate surface molecule print obtained
Polymer has good specific recognition effect to template molecule.
Embodiment 13
It is small that kitasamycin tartrate molecular imprinted polymer on surface obtained in embodiment 1 is filled in the polypropylene equipped with sieve plate
Column adds to the separation and concentration pretreatment of trace residue macrolides pollutant monitoring in actual waste water and animal food
Mark laboratory test results show that the rate of recovery reaches 90% or more, show gained kitasamycin tartrate molecular imprinted polymer on surface
With good practicability；It is recycled by 30 times or more, the absorption property of the solid phase extraction column shows without being decreased obviously
Gained kitasamycin tartrate molecular imprinted polymer on surface has excellent regenerative adsorption performance.
Embodiment above describes basic principles and main features of the invention and advantage, the technical staff of the industry should
Understand, the present invention is not limited to the above embodiments, and the above embodiments and description only describe originals of the invention
Reason, under the range for not departing from the principle of the invention, various changes and improvements may be made to the invention, these changes and improvements are each fallen within
In the scope of protection of the invention.
Claims (6)
1. a kind of aqueous phase preparation method of kitasamycin tartrate molecular imprinted polymer on surface, it is characterised in that detailed process
Are as follows: function monomer [VAIM] Cl and/or AMPS and template molecule kitasamycin tartrate are dissolved in added with stryrene divinyl
In the pure water solvent of base benzene carrier granular, crosslinking agent N, N '-methylene-bisacrylamide and initiation are added after 30 DEG C of prepolymerizations
Agent azodiisobutyronitrile passes through crosslinking agent N, two isobutyl of N '-methylene-bisacrylamide and initiator azo under nitrogen protection
The collective effect of nitrile is mould in styrene-divinylbenzene carrier particle surface cladding tartaric acid guitar in 60 DEG C of generation polymerization reactions
Plain molecular imprinted polymer on surface layer, the kitasamycin tartrate molecular imprinted polymer on surface being capable of Selective recognition water environments
Macrolide antibiotics pollutant in sample.
2. the aqueous phase preparation method of kitasamycin tartrate molecular imprinted polymer on surface according to claim 1, special
Sign is: the mass ratio that feeds intake of the template molecule and carrier granular is 1.1-1.4:1.
3. the aqueous phase preparation method of kitasamycin tartrate molecular imprinted polymer on surface according to claim 1, special
Sign is: the molar ratio of function monomer [VAIM] Cl and AMPS is 0-3:3-0.
4. the aqueous phase preparation method of kitasamycin tartrate molecular imprinted polymer on surface according to claim 1, special
Sign is: the template molecule, function monomer, crosslinking agent and initiator molar ratio be 1:4-8:15-25:0.24.
5. the aqueous phase preparation method of kitasamycin tartrate molecular imprinted polymer on surface according to claim 1, special
Sign is specific steps are as follows: template molecule kitasamycin tartrate and function monomer [VAIM] Cl and AMPS are dissolved in 25 mL
In pure water solvent, 0.15 g styrene-divinylbenzene carrier granular is added, wherein the quality of template molecule and carrier granular
Than for 1.4:1, sealing, after vibrating 4 h of prepolymerization in 30 DEG C of water-baths, with template molecule, function monomer, crosslinking agent and initiator
Crosslinking agent MBA and initiator A IBN is added in the proportion that molar ratio is 1:4:20:0.24, in 60 DEG C of water after ultrasound, logical nitrogen, sealing
In bath oscillation polymerization 20 h, products therefrom with methanol rinse dry after be 8:2 with volume ratio methanol and acetic acid mixed liquor rope
Family name extracts 40 h, obtains kitasamycin tartrate molecular imprinted polymer on surface in 60 DEG C of vacuum drying after again with methanol flushing,
It is with 10 mL molar concentrations by kitasamycin tartrate molecular imprinted polymer on surface made from 10 mg and non-imprinted polymer
0.2 mmol L-1Kitasamycin tartrate aqueous solution mixing, shaken at room temperature adsorb 3 h after, take supernatant with ultraviolet spectrometry light
Absorbance value under 232 nm of degree meter detection, is computed, the kitasamycin tartrate molecular imprinted polymer on surface is to aqueous solution
The adsorbance of mesotartaric acid kitasamycin is 80.87 μm of ol g-1。
6. kitasamycin tartrate surface molecule print made from method described in any one of -5 polymerize according to claim 1
The application of object macrolide antibiotics pollutant in Selective recognition environmental water sample, wherein kitasamycin tartrate surface
Molecularly imprinted polymer uniform particle sizes have the work in the 3 D stereo hole and specific bond that match with kitasamycin tartrate
With site, it can be used in the Selective Separation and enrichment of micro macrolide antibiotics pollutant in Complex Water Environment sample,
Kitasamycin tartrate molecular imprinted polymer on surface repetitive cycling after regeneration uses.
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