CN109874933A - A kind of high copper feed - Google Patents

Abstract

A kind of high copper feed.The preparation method is that: soybean oil and cocoa butter heating and melting are weighed in proportion in half fluidised form rouge material；Raw material are weighed again: casein, cornstarch, maltodextrin, sucrose, cellulose, l-cysteine, composite mineral matter, calcium phosphate dibasic anhydrous, calcium carbonate, monohydrate potassium potassium, multi-vitamins and choline chloride；The weighed raw material of institute are mixed, it is 10mm that diameter, which is made, by ring die pelleter, and length is the feed granules of 10~20mm；24~48h of removal moisture drying is carried out by dehumidifier again again, it is final must dry after feed granules；By product packaging, irradiation sterilization is carried out with Co 60, obtains finished product.Product of the present invention nutritional ingredient is stablized, and the hardness and mouthfeel of rodent eating habits are met；Model success rate in the present invention is high, and process is simple, good at fruit is imitated.

Description

A kind of high copper feed

Technical field

The present invention relates to building pharmaceutical experiment animal model technical fields.

Background technique

Copper is the essential trace elements of the human body, participates in a variety of important biomolecule enzymatic synthesis as prothetic group.Normal adult daily from Diet regimen 2~5mg of copper, about 30% in Stomach duodenum and jejunum upper end absorbed into serum, the most of and loose combination of albumin Into liver cell, copper and alpha2 Globulin are firmly combined at ceruloplasmin (ceruloplasmin, CP) in liver cell, and CP is aerobic Change enzymatic activity, is inNavy blue, residual copper is incorporated into other special cupreins.The daily bile of normal person arranges about 1200 μ of amount of copper g.About 70% CP is present in blood plasma, remaining is present in outside blood vessel, and 90%~95% copper is incorporated on CP in blood circulation.CP There is important physiological function, the donor that can be used as copper participates in cromoci and the synthesis of other cupreins, and there is ferrous oxidase to make With, by ferrous oxidation be high-speed rail state, make hydrogen reduction Cheng Shui.Remaining copper is excreted by bile, urine and sweat.Liver beans Shape nuclear degeneration patient's ceruloplasmin dyssynthesis, 90% or more patients serum's CP amount significantly reduces, but preceding ceruloplasmin in liver (Apo-CP) content and structure are normal, and prompting biochemical disorders that Apo-CP link in conjunction with copper, CP dyssynthesis in liver occurs is The basic genetic defect of this disease.Copper metabolic disorder causes change of serum C P dyssynthesis in liver, and CP is caused to reduce, and cupruresis increases, Biliary tract is arranged copper and is reduced, and excess copper causes a disease in tissue depositions such as liver, brain, kidney and corneas.

Normal hepatocytes copper content is 50 μ g/g dry weights, and hepatolenticular degeneration patient's liver copper content is mostly 250 μ g or more/g dry Weight.The normal reference value of liver function main indicator glutamic-pyruvic transaminase is 0~40U/L.Hepatolenticular degeneration patient usually urinates copper Measure>200 μ g/24h (normal<50 μ g/24h).

Hepatolenticular degeneration animal model can preferable simulation liver copper deposition induced liver injury pathologic process, and have The features such as simple and practical, reproducible is to study the ideal animal model of copper dysbolism disease.

Currently, domestic common high dose copper-bath carries out stomach-filling to rodent and makes hepatolenticular degeneration animal Model；The shortcomings that administration by gavage is: 1, the dosage that in use although administration by gavage uses is accurate, but in operation can Certain stimulation is caused to the body of animal, be especially easy to injure alimentary canal and is easy drug being strayed into tracheae, to cause Since breathing asphyxia causes the abnormalities of animal dead, and rodent free choice feeding is not met using administration by gavage yet Eating habits；2, the heavy workload that staff not only operates in operation increases the work load of staff, when long Between operation be easy to appear mistake, increase and increase the unstability of experimental result so as to cause the death rate of animal；3, Administration by gavage is easy to cause experimental animal to generate stress reaction, and be also easy to cause phenotype is unstable, experimental data has deviation and It is low at mould rate；By the present invention in that with the high-copper forage feed rodent for strengthening copper carbonate, in preparation process using it is single, Stablize the stable feed of the nutritional ingredient of nutrient composition, such feed granules have good forming effect, hardness strong, not fragile Broken feature not only ensure that the food ration of animal, and preferably guarantee the process of grinding one's teeth in sleep of rodent itself；Meet and nibbles The hardness and mouthfeel of tooth animal eating habits, the hepatolenticular degeneration animal model success rate that the present invention induces are high.

Summary of the invention

The present invention a kind of constituted while being strengthened by the nutrient of single stable in view of the deficienciess of the prior art, providing The modeling feed of copper carbonate (being 25~30 times of chow diet), the feed construct the success rate of hepatolenticular degeneration animal model It is high, good, repeatable strong at fruit is imitated.

To achieve the above object, a kind of the technical solution adopted by the present invention are as follows: building hepatolenticular degeneration animal model High copper feed is made of the component of following weight ratio: casein 15%~30%, cornstarch 30%~50%, maltodextrin 10%~25%, sucrose 15%~30%, cellulose 4%~10%, soybean oil 1%~8%, cocoa butter 0.5%~7%, L- Cystine 0.1%~1%, composite mineral matter 0.5%~4%, calcium phosphate dibasic anhydrous 1%~3%, calcium carbonate 0.2%~1%, Monohydrate potassium potassium 0.5%~3%, multi-vitamins 0.5%~3% and choline chloride 0.2%~1%.

The multi-vitamins are made of by weight following components: vitamine A acetate 0.0005%~0.005%, Vitamin D₃0.005%~0.05%, Vitwas E 0.08%~0.3%, vitamin K₁0.0001%~0.001%, Biotin 0.001%~0.05%, vitamin B₁₂0.001%~0.01%, folic acid 0.0001%~0.001%, niacin 0.007%~0.07%, calcium pantothenate 0.005%~0.01%, vitamin B₆0.0001%~0.001%, vitamin B₂0.0001%~0.001%, vitamin B₁0.0001%~0.001% and cornstarch 0.4%~2.5%.

Preparation method includes the following steps:

1), pre-treatment of raw material,

Firstly, weigh soybean oil 1%~8%, cocoa butter 0.5%~7%, soybean oil and cocoa butter are heated to 80~ 90 DEG C of thawings, are added the water of raw material total weight 5%~12%, stir evenly, and are in half fluidised form rouge material；It shelves stand-by；

Proportionally weigh raw material: casein, maltodextrin, sucrose, cellulose, L- cystine, is answered at cornstarch Close minerals, calcium phosphate dibasic anhydrous, calcium carbonate, monohydrate potassium potassium, multi-vitamins and choline chloride；

2) mixture, is prepared,

The weighed raw material of institute are mixed, stirs evenly, above-mentioned half fluidised form rouge material is added, continues to mix 2~4 minutes；

3) product, is prepared,

At 20~30 DEG C, it is 10mm that diameter, which is made, by ring die pelleter, and length is the feed granules of 10~20mm；? At 18~25 DEG C, feed granules are subjected to 24~48h of removal moisture drying by dehumidifier again, it is final must dry after feed granules, feeding The content for expecting moisture in particle is 5%~10%；

4) finished product, is obtained,

The said goods are packed, irradiation sterilization is carried out with Co 60, obtains finished product.

Co-60 radiation source generates gamma-rays, acts on microorganism, can directly or indirectly destroy the nucleus of microorganism, To kill microorganism, play the role of disinfection and sterilization.

Wherein: casein: having pH sensibility, can solidify under the acidic environment of stomach and become gel-like therefore junket egg White digestibility is slower；Digestion has many benefits slowly, can protect for a long time, repair and nourish body.

Cornstarch: nutrient rich in contains a large amount of lecithin, linoleic acid, grain alcohol, vitamin in corn E, cellulose etc. has the plurality kinds of health care such as lowering blood pressure and blood fat, anti arteriosclerosis, prevention intestinal cancer, beautifying face and moistering lotion, anti-aging Effect.

Maltodextrin: have thickening property strong, carrier-mediated good, Fermented is small, and filling effect is good, and nonhygroscopic, free from extraneous odour easily disappears The features such as change, low-heat, low sugariness.Maltodextrin contains a large amount of polysaccharide, in addition also containing micro- beneficial to human body such as calcium, iron Secondary element and minerals, and human normal can be promotedMetabolism。

Sucrose: being photosynthetic primary product, is the principal mode of plant storage, accumulation and transport sugar, You Yifen Sub- glucose and a molecule fructose dehydrating condensation are formed, soluble easily in water to be relatively insoluble in ethyl alcohol.

Cellulose: it is unobstructed to be able to maintain enteron aisle, toxin expelling defaecation, safeguards skin health.

Soybean oil: vitamin E containing palmitinic acid, stearic acid, arachidic acid, oleic acid, linoleic acid, linolenic acid and volume, Vitamin D, vitamin A, carrotene, calcium, phosphorus, iron and lecithin abundant, digestibility are up to 98%.It is solid to can promote gallbladder Alcohol decomposes excretion, reduces Blood Cholesterol in the deposition of vascular wall, reduces incidence of cardiovascular disease, protect body, promotes big The growth and development of brain, nerve.

Cocoa butter: be fromCocoa liquorThe milk yellow hardness natural plant grease of middle taking-up is a kind of very unique oil Rouge.It is a kind of existing hardness, dissolves to obtain fast grease again.Cocoa butter is known most stable ofEdible oil, containing can prevent from going bad 'sNatural。

L-cysteine: having and body cell promoted to aoxidize and restore function, increases white blood cell and prevents the works such as pathogen development With.

Choline chloride: one of choline chloride category B family vitamin, it is acetylcholine in biological tissue, lecithin and nerve The component part of phosphide, the in animal body metabolism and conversion of adjustable fat can prevent to deposit and its organize in liver It is denaturalized the donor as a kind of methyl, re-forming for amino acid is can promote, improves the utilization rate of amino acid.

Copper carbonate: providing copper, and copper is the essential trace elements of the human body, participates in a variety of important biomolecule enzymes as prothetic group and closes At.

Calcium phosphate dibasic anhydrous: the mineral matter nutritionals such as phosphorus, calcium, animal pole absorption easy to digest are mainly provided.Animal can be accelerated raw Long development shortens fattening period, quickly increases weight；The breeding rate and survival rate of animal can be improved, while having enhancing animal disease resistant resistance to Cold ability has preventive and therapeutic effect to the rickets of livestock and poultry, dysentery characterized by white mucous stool disease, paralysed disease disease.

Calcium carbonate: calcium supplement absorptivity can be made up to 39%, be only second toCalcium citrate malateDissolve in gastric acid, it has also become dosage form is most It is more, using most calcium supplements.

Monohydrate potassium potassium: also known as potassium citrate, be used as buffer, chelating agent, stabilizer, antioxidant, emulsifier, Flavoring agent etc..

Vitamin D₃: absorption of the human body to calcium, phosphorus is improved, plasma calcium and the level of blood plasma phosphorus is made to reach degree of saturation；Promote Into growth and bone calcification, promote tooth sound；Increase the absorption of phosphorus by intestinal wall, and inhaling again for phosphorus is increased by renal tubule It receives；Maintain the normal level of citrate in blood；Vitamin D₃It is active 7- dehydrocholesterol.

Vitamin K₁: liver can be promoted to synthesize factor, be used forHypoprothrombinemia；Also there is analgesia, alleviate branch The effect of tracheospasm has obvious effects on colic pain caused by visceral smooth muscle colic pain, cholepathia spastica, enterospasm；It can be with Additive for multidimensional food and poultry and livestock feed.

Biotin: be fat and the indispensable substance of protein eubolism, maintain the complete of epithelial structures and It is sound；Enhance organism immune response and resistance；Maintain normal growth and development.

Niacin: being converted into niacinamide usp in vivo, and niacinamide usp is the component part of coenzyme, participates in HypercholesterolemicRats, tissue The oxidation engineering of breathing and the process of carbohydrate anaerobic decomposition.

Calcium pantothenate: for medicine, food andFeed addictive.It isCoacetylaseIngredient, participate in carbohydrate, fat and The metabolism of protein.

Beneficial effects of the present invention: in modeling feed of the present invention, strengthening carbonic acid copper content (is the 25~30 of chow diet Times, copper content is 10mg/kg in chow diet), rodent is fed by high copper feed, induces hepatolenticular degeneration mould Type, the pathologic process of preferable simulation liver copper deposition induced liver injury.Product using single stable nutrient constitute, nutrition at Divide and stablize, meets the hardness and mouthfeel of rodent eating habits.The present invention constructs model success rate height, and process is simple, Cheng Mo Effect is good.

Detailed description of the invention

Fig. 1 is preparation flow schematic diagram of the invention；

Fig. 2 is Wistar rat blood serum copper (Cu) content curve graph；

Fig. 3 is Wistar liver tissues of rats copper (Cu) content curve graph；

Fig. 4 is Wistar rat blood serum glutamic-pyruvic transaminase (ALT) horizontal line graph.

Specific embodiment

Below with regard to specific embodiment, the present invention is furture elucidated, it should be understood that these embodiments are merely to illustrate this hair Bright rather than limit the scope of the invention, after the present invention has been read, those skilled in the art are to of the invention various etc. The modification of valence form falls within the application range as defined in the appended claims.

In order to understand the present invention, below the present invention is further described in detail.

A kind of high copper feed constructing hepatolenticular degeneration animal model, is made of: casein the component of following weight ratio 15%~30%, cornstarch 30%~50%, maltodextrin 10%~25%, sucrose 15%~30%, cellulose 4%~ 10%, soybean oil 1%~8%, cocoa butter 0.5%~7%, l-cysteine 0.1%~1%, composite mineral matter 0.5%~4%, Calcium phosphate dibasic anhydrous 1%~3%, calcium carbonate 0.2%~1%, monohydrate potassium potassium 0.5%~3%, multi-vitamins 0.5%~3% and choline chloride 0.2%~1%.

The composite mineral matter is made of by weight following components: sodium chloride 0.1%~1%, magnesia 0.01%~ 0.2%, magnesium sulfate 0.1%~1%, Ammonium Molybdate Tetrahydrate 0.0001%~0.008%, potassium chromium sulfate 0.004%~0.01%, Copper carbonate 0.02%~0.1%, ironic citrate 0.05%~0.5%, manganese carbonate hydrate 0.01%~0.1%, Potassiumiodate 0.00005%~0.0005%, sodium fluoride 0.0008%~0.001%, sodium selenite 0.00005%~0.0005%, carbonic acid Zinc 0.005%~0.08% and cornstarch 0.2%~1%.

The multi-vitamins are made of by weight following components: vitamine A acetate 0.0005%~0.005%, Vitamin D₃0.005%~0.05%, Vitwas E 0.08%~0.3%, vitamin K₁0.0001%~0.001%, Biotin 0.001%~0.05%, vitamin B₁₂0.001%~0.01%, folic acid 0.0001%~0.001%, niacin 0.007%~0.07%, calcium pantothenate 0.005%~0.01%, vitamin B₆0.0001%~0.001%, vitamin B₂0.0001%~0.001%, vitamin B₁0.0001%~0.001% and cornstarch 0.4%~2.5%.

Preparation method includes the following steps:

1), pre-treatment of raw material,

Firstly, weigh soybean oil 1%~8%, cocoa butter 0.5%~7%, soybean oil and cocoa butter are heated to 80~ 90 DEG C of thawings, are added the water of raw material total weight 5%~12%, stir evenly, and are in half fluidised form rouge material；It shelves stand-by；

Proportionally weigh raw material: casein, maltodextrin, sucrose, cellulose, L- cystine, is answered at cornstarch Close minerals, calcium phosphate dibasic anhydrous, calcium carbonate, monohydrate potassium potassium, multi-vitamins and choline chloride；

2) mixture, is prepared,

The weighed raw material of institute are mixed, stirs evenly, above-mentioned half fluidised form rouge material is added, continues to mix 2~4 minutes；

3) product, is prepared,

At 20~30 DEG C, it is 10mm that diameter, which is made, by ring die pelleter, and length is the feed granules of 10~20mm；? At 18~25 DEG C, feed granules are subjected to 24~48h of removal moisture drying by dehumidifier again, it is final must dry after feed granules, feeding The content for expecting moisture in particle is 5%~10%；

4) finished product, is obtained,

The said goods are packed, irradiation sterilization is carried out with Co 60, obtains finished product.

Specific embodiment one

1), pre-treatment of raw material,

Firstly, weighing the material in raw material: soybean oil and cocoa butter are heated to by soybean oil 1%, cocoa butter 0.5% 80 DEG C melt, and the water of raw material total weight 5% is added in the soybean oil and cocoa lipoprotein mixture after heating and melting, and stirring is equal It is even, it is in half fluidised form rouge material；It shelves stand-by；

Proportionally weigh in raw material other materials: casein 15%, cornstarch 30%, maltodextrin 10%, Sucrose 15%, cellulose 4%, l-cysteine 0.1%, composite mineral matter 0.5%, calcium phosphate dibasic anhydrous 1%, calcium carbonate 0.2%, Monohydrate potassium potassium 0.5%, multi-vitamins 0.5% and choline chloride 0.2%；

2) mixture, is prepared,

The above-mentioned weighed raw material of institute are mixed, stirs evenly, above-mentioned half fluidised form rouge material is added, continues to mix 2~4 points Clock；

3) product, is prepared,

At 20~30 DEG C, it is 10mm that diameter, which is made, by ring die pelleter, and length is the feed granules of 10~20mm；? At 18~25 DEG C, feed granules are subjected to 24~48h of removal moisture drying by dehumidifier again, it is final must dry after feed granules, feeding The content for expecting moisture in particle is 5%~10%；

4) finished product, is obtained,

The said goods are packed, irradiation sterilization is carried out with Co 60, obtains finished product.

Specific embodiment two:

1), pre-treatment of raw material,

Firstly, weighing the material in raw material: soybean oil and cocoa butter are heated to 90 by soybean oil 8%, cocoa butter 7% DEG C melt, the water of raw material total weight 12% is added in the soybean oil and cocoa lipoprotein mixture toward heating and melting after, stirring is It is even, it is in half fluidised form rouge material；It shelves stand-by；

Proportionally weigh in raw material other materials: casein 30%, cornstarch 50%, maltodextrin 25%, Sucrose 30%, cellulose 10%, l-cysteine 1%, composite mineral matter 4%, calcium phosphate dibasic anhydrous 3%, calcium carbonate 1%, a water Close potassium citrate 3%, multi-vitamins 3% and choline chloride 1%；

2) mixture, is prepared,

The weighed raw material of institute are mixed, stirs evenly, above-mentioned half fluidised form rouge material is added, continues to mix 2~4 minutes；

3) product, is prepared,

At 20~30 DEG C, it is 10mm that diameter, which is made, by ring die pelleter, and length is the feed granules of 10~20mm；? At 18~25 DEG C, feed granules are subjected to 24~48h of removal moisture drying by dehumidifier again, it is final must dry after feed granules, feeding The content for expecting moisture in particle is 5%~10%；

4) finished product, is obtained,

The said goods are packed, irradiation sterilization is carried out with Co 60, obtains finished product.

Specific embodiment three:

1), pre-treatment of raw material,

Firstly, weighing the material in raw material: soybean oil 4.5%, cocoa butter 3.8% heat soybean oil and cocoa butter Melt to 85 DEG C, the water of raw material total weight 9% is added in the soybean oil and cocoa lipoprotein mixture after heating and melting, stirring is equal It is even, it is in half fluidised form rouge material；It shelves stand-by；

Proportionally weigh in raw material other materials: casein 23%, cornstarch 40%, maltodextrin 18%, Sucrose 23%, cellulose 7%, l-cysteine 0.6%, composite mineral matter 2.3%, calcium phosphate dibasic anhydrous 2%, calcium carbonate 0.6%, Monohydrate potassium potassium 1.8%, multi-vitamins 1.8% and choline chloride 0.6%.

2) mixture, is prepared,

The weighed raw material of institute are mixed, stirs evenly, above-mentioned half fluidised form rouge material is added, continues to mix 2~4 minutes；

3) product, is prepared,

At 20~30 DEG C, it is 10mm that diameter, which is made, by ring die pelleter, and length is the feed granules of 10~20mm；? At 18~25 DEG C, feed granules are subjected to 24~48h of removal moisture drying by dehumidifier again, it is final must dry after feed granules, feeding The content for expecting moisture in particle is 5%~10%；

4) finished product, is obtained,

The said goods are packed, irradiation sterilization is carried out with Co 60, obtains finished product.

Show that the present invention uses the high-copper that copper carbonate is made of while strengthened the nutrient of single stable to raise through animal experiment Material；It is high to construct model success rate, modelling process is simple, good at fruit is imitated；Repeatable, stability waits well and is animal experiment Data proved, related animal experiment data of the invention:

1. prepared by animal model

Healthy male Wistar rat, 40, at 3 monthly ages, rat is divided into model group and control at random by 210 ± 25g of weight Group.Model group rats feed the high copper feed of the present invention, and control rats feed normal diet, the equal ad lib of each group, drinking-water.Hello It supports 12 weeks；Detect serum and hepatic tissue copper content, serum glutamic pyruvic transminase (ALT).

2. the measuring method of serum and hepatic tissue copper (Cu) and serum glutamic pyruvic transminase (ALT)

Serum copper detection mixes after being diluted with test serum with deionized water 1:10, is surveyed using atomic absorption spectrophotometer Amount.300~500mg of hepatic tissue is first cleaned with 0.9%NaC1 repeatedly, then is blotted with clean filter paper, with concentrated nitric acid (analysis is pure) 10mL low-temperature heat is digested, and tissues is waited to be completely dissolved to yellow clear, surveys copper content with atomic absorption method.Serum Automatic biochemical analyzer measurement glutamic-pyruvic transaminase (ALT) on sample.

3. experimental result

1), the variation of liver tissues of rats

Control rats lobuli hepatis is normal, and central vein, hepatic sinusoid are clear in structure, the visible mitochondria of model group rats liver Swelling, reticulum dilatation, lysosome particle increased significantly.Wistar rat is fed high copper feed 12 weeks, a large amount of copper of hepatic tissue Ion deposition, the up to decades of times of control group, copper ion can lead to the destruction of liver cell in the excess deposition of liver.

2), the variation of serum and hepatic tissue copper (Cu) content and serum glutamic pyruvic transminase (ALT)

The serum copper content of model group rats is (4.68 ± 0.37mg/mL), is significantly higher than control group (1.99 ± 0.31 mg/mL)；The hepatic tissue copper content of model group rats be (15.93 ± 3.22mg/kg), be significantly higher than control group (0.41 ± 0.23mg/kg), illustrate the extension with the high-copper forage feed time, hepatic tissue and serum copper content increase, but the copper of hepatic tissue Content increasing degree is bigger.The horizontal serum glutamic pyruvic transminase (ALT) of model group rats is (198.27 ± 15.42U/L), hence it is evident that Higher than control group (37.06 ± 1.13U/L).The glutamic-pyruvic transaminase of model group rats largely increases, and hepatocellular damage is serious.

Beneficial effects of the present invention: in modeling feed of the present invention, strengthening carbonic acid copper content (is the 25~30 of chow diet Times, copper content is 10mg/kg in chow diet), rodent is fed by high copper feed, induces hepatolenticular degeneration mould Type, the pathologic process of preferable simulation liver copper deposition induced liver injury.Product using single stable nutrient constitute, nutrition at Divide and stablize, meets the hardness and mouthfeel of rodent eating habits.The present invention constructs model success rate height, and process is simple, Cheng Mo Effect is good.

Claims (3)

1. a kind of high copper feed, which is characterized in that be made of the component of following weight ratio: casein 15%~30%, corn form sediment Powder 30%~50%, maltodextrin 10%~25%, sucrose 15%~30%, cellulose 4%~10%, soybean oil 1%~ 8%, cocoa butter 0.5%~7%, l-cysteine 0.1%~1%, composite mineral matter 0.5%~4%, calcium phosphate dibasic anhydrous 1% ~3%, calcium carbonate 0.2%~1%, monohydrate potassium potassium 0.5%~3%, multi-vitamins 0.5%~3% and chlorination gallbladder Alkali 0.2%~1%.

2. a kind of high copper feed according to claim 1 is it is characterized in that, the multi-vitamins press weight by following components Amount is than composition: vitamine A acetate 0.0005%~0.005%, vitamin D₃0.005%~0.05%, Vitwas E 0.08%~0.3%, vitamin K₁0.0001%~0.001%, biotin 0.001%~0.05%, vitamin B₁₂0.001% ~0.01%, folic acid 0.0001%~0.001%, niacin 0.007%~0.07%, calcium pantothenate 0.005%~0.01%, dimension life Plain B₆0.0001%~0.001%, vitamin B₂0.0001%~0.001%, vitamin B₁0.0001%~0.001% and beautiful Rice starch 0.4%~2.5%.

3. a kind of a kind of preparation method of high copper feed described in claim 1, which comprises the following steps:

1), pre-treatment of raw material,

Firstly, weighing soybean oil 1%~8%, soybean oil and cocoa butter are heated to 80~90 DEG C and melted by cocoa butter 0.5%~7% Change, the water of raw material total weight 5%~12% is added, stirs evenly, is in half fluidised form rouge material；It shelves stand-by；

Proportionally weigh raw material: casein, cornstarch, maltodextrin, sucrose, cellulose, l-cysteine, complex mineral Matter, calcium phosphate dibasic anhydrous, calcium carbonate, monohydrate potassium potassium, multi-vitamins and choline chloride；

2) mixture, is prepared,

The weighed raw material of institute are mixed, stirs evenly, above-mentioned half fluidised form rouge material is added, continues to mix 2~4 minutes；

3) product, is prepared,

At 20~30 DEG C, it is 10mm that diameter, which is made, by ring die pelleter, and length is the feed granules of 10~20mm；18~ At 25 DEG C, feed granules are subjected to 24~48h of removal moisture drying by dehumidifier again, it is final must dry after feed granules, feed The content of moisture is 5%~10% in grain；

4) finished product, is obtained,

The said goods are packed, irradiation sterilization is carried out with Co 60, obtains finished product.