CN109868221A - A kind of biotechnology cell culture apparatus - Google Patents
A kind of biotechnology cell culture apparatus Download PDFInfo
- Publication number
- CN109868221A CN109868221A CN201910204106.6A CN201910204106A CN109868221A CN 109868221 A CN109868221 A CN 109868221A CN 201910204106 A CN201910204106 A CN 201910204106A CN 109868221 A CN109868221 A CN 109868221A
- Authority
- CN
- China
- Prior art keywords
- incubator
- control device
- circuit control
- culture
- exhaust pipe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 238000004113 cell culture Methods 0.000 title claims abstract description 22
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 30
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 22
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 22
- 239000001301 oxygen Substances 0.000 claims abstract description 22
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 15
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 15
- 238000012258 culturing Methods 0.000 claims abstract description 14
- 239000002912 waste gas Substances 0.000 claims abstract description 10
- 239000007789 gas Substances 0.000 claims abstract description 8
- 230000005611 electricity Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims 1
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 239000012530 fluid Substances 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 abstract description 2
- 238000012544 monitoring process Methods 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 7
- 239000006174 pH buffer Substances 0.000 description 3
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 239000012774 insulation material Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 239000012930 cell culture fluid Substances 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention provides a kind of biotechnology cell culture apparatus, including incubator, culturing rack, motor base, waste gas filter, oxygen storage device, exhaust pipe, air blower, oxygen cathete, solenoid valve, carbon dioxide sensor, circuit control device and power supply, the beneficial effects of the present invention are: exhaust gas is excluded by exhaust pipe by filtered fluid again, and unlatching incubator bring has been blocked to pollute；Culture environment, the automatic blower of circuit control device and solenoid valve in carbon dioxide sensor and temperature sensor monitoring incubator, it is accurate to control incubator composition of gases within；Motor base can drive culturing rack to rotate, and make environment locating for culture cell more uniform.
Description
Technical field
The present invention relates to a kind of biotechnologies, and in particular to a kind of biology cell culture apparatus.
Background technique
Currently, cell culture technology is also cell clone technology, the regular noun in biology is cell culture technology.
Though for entire biotechnology, or for one of them biological cloning technology, cell culture be all one must not
The process that can lack, the extensive clone of cell culture inherently cell.Cell culture had both included the culture of microbial cell,
Cell culture technology can become simple many cells that are unicellular or seldom breaking up by mass propgation by a cell, this is
The essential link of clone technology, and the clone of cell culture inherently cell.It is obtained largely by cell culture
Cell or its metabolite.Because biological product is got from cell, it is possible to say that cell culture technology is biotechnology
In most crucial, most basic technology.Cell culture work has been widely used in each neck such as biology, medicine, new drug development
Domain becomes one of most important basic science.
The pH of general cell culture fluid is between 7.0-7.4.Since carbonate pH buffer system is a kind of physiological pH buffering
System (it is most important pH buffer system in blood of human body), so most of culture solutions keep stable pH with it.With
When powder prepares culture solution, it is often necessary to a certain amount of sodium bicarbonate be added.To most of using carbonate as pH buffer system
Culture solution for, in order to maintain stable pH, the carbon dioxide in incubator needs to maintain between 2-10%, with keep
The concentration of the carbon dioxide dissolved in culture solution.The vessel needs of cell culture simultaneously are a degree of ventilative, in order to gas
Exchange.Therefore each gaseous substance concentration for how accurately controlling cell culture apparatus is urgently to be resolved.
Summary of the invention
To solve the above problems, the present invention provides a kind of biotechnology cell culture apparatus, including incubator, culture
Frame, motor base, waste gas filter, oxygen storage device, exhaust pipe, air blower, oxygen cathete, solenoid valve, carbon dioxide sensor,
Circuit control device and power supply, the incubator is interior to be equipped with culture chamber, and the culturing rack is mounted on the motor base, described
Motor base is mounted on the culture chamber surface, the incubator respectively with the waste gas filter and the oxygen storage device phase
It contacts and is contacting position equipped with aperture, the exhaust pipe first end is connected with the incubator aperture, the exhaust
Pipe second end, which passes through the waste gas filter and from top to bottom immerses, filters liquid layer in it, the oxygen storage device passes through oxygen cathete
It is connected across aperture with the incubator, the air blower is mounted on the culture corresponding with the exhaust pipe first end
Chamber inner sidewall, the solenoid valve are mounted on the culture chamber inner sidewall and are connected with the oxygen cathete, the carbon dioxide sensing
Device is mounted on the culturing rack, and the circuit control device and the power supply are mounted on the incubator lateral wall, the electricity
Machine base, the air blower, the solenoid valve, the carbon dioxide sensor and the power supply are filled with the circuit control
Set electrical connection.
More preferably, the culture box material is thermal insulation material.
More preferably, the circuit control device further includes temperature sensor and touch control display, the temperature sensor peace
On the culturing rack, the touch control display is mounted on the incubator one side wall, the temperature sensor and the touching
Control display is electrically connected with the circuit control device ontology.
The beneficial effects of the present invention are: exhaust gas is excluded by exhaust pipe by filtered fluid again, has blocked unlatching incubator
Bring pollution；Culture environment in carbon dioxide sensor and temperature sensor monitoring incubator, circuit control device drive automatically
Dynamic air blower and solenoid valve, it is accurate to control incubator composition of gases within；Motor base can drive culturing rack to rotate, and make culture thin
Environment locating for born of the same parents is more uniform.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of biotechnology cell culture apparatus of the present invention.
In figure: 1, incubator, 2, culturing rack, 3, motor base, 4, waste gas filter, 5, oxygen storage device, 6, exhaust pipe,
7, air blower, 8, oxygen cathete, 9, solenoid valve, 10, carbon dioxide sensor, 11-1, temperature sensor.
Specific embodiment
Scheme in the present invention to clearly illustrate, is given below preferred embodiment and is described with reference to the accompanying drawings.
Embodiment 1
As shown in Figure 1, to solve the above problems, the present invention provides a kind of biotechnology cell culture apparatus, including training
Support case 1, culturing rack 2, motor base 3, waste gas filter 4, oxygen storage device 5, exhaust pipe 6, air blower 7, oxygen cathete 8, electromagnetism
Valve 9, carbon dioxide sensor 10, circuit control device 11 and power supply 12, the incubator 1 is interior to be equipped with culture chamber 1-1, the training
It supports frame 2 to be mounted on the motor base 3, the motor base 3 is mounted on the surface the culture chamber 1-1, and the incubator 1 divides
It is not in contact with the waste gas filter 4 and the oxygen storage device 5 and is contacting position equipped with aperture, the exhaust
6 first end of pipe is connected with 1 aperture of incubator, 6 second end of exhaust pipe pass through the waste gas filter 4 and by upper and
Lower immerse filters liquid layer in it, the oxygen storage device 5 passes through aperture by oxygen cathete 8 and is connected with the incubator 1, the drum
Blower 7 is mounted on the culture chamber 1-1 inner sidewall corresponding with 6 first end of exhaust pipe, and the solenoid valve 9 is mounted on institute
It states culture chamber 1-1 inner sidewall and is connected with the oxygen cathete 8, the carbon dioxide sensor 10 is mounted on the culturing rack 2,
The circuit control device 11 and the power supply 12 are mounted on 1 lateral wall of incubator, the motor base 3, the air blast
Machine 7, the solenoid valve 9, the carbon dioxide sensor 10 and the power supply 12 are electrically connected with the circuit control device 11
It connects.
More preferably, 1 material of incubator is thermal insulation material.
More preferably, the circuit control device 11 further includes temperature sensor 11-1 and touch control display 11-2, the temperature
Degree sensor 11-1 is mounted on the culturing rack 2, and the touch control display 11-2 is mounted on 1 one side wall of incubator, institute
It states temperature sensor 11-1 and the touch control display 11-2 is electrically connected with the circuit control device ontology 11.
In use, checking the environmental parameter of entire culture apparatus by touch control display, automatic control or manual may be programmed
Entire culture apparatus operation is controlled, solenoid valve, starting electricity are opened when detecting gas concentration lwevel or temperature higher than predetermined critical line
Machine base open simultaneously air blower to culture chamber take a breath, when gas concentration lwevel or temperature are in normal range (NR) close solenoid valve,
Motor base and air blower.
Finally it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than the present invention is protected
The limitation of range is protected, although explaining in detail referring to preferred embodiment to the present invention, those skilled in the art are answered
Work as understanding, it can be with modification or equivalent replacement of the technical solution of the present invention are made, without departing from the reality of technical solution of the present invention
Matter and range.
Claims (3)
1. a kind of biotechnology cell culture apparatus characterized by comprising incubator, culturing rack, motor base, exhaust gas
Filter device, oxygen storage device, exhaust pipe, air blower, oxygen cathete, solenoid valve, carbon dioxide sensor, circuit control device and electricity
Source, the incubator is interior to be equipped with culture chamber, and the culturing rack is mounted on the motor base, and the motor base is mounted on institute
Culture chamber surface is stated, the incubator is in contact with the waste gas filter and the oxygen storage device respectively and connects with it
Contact portion position is equipped with aperture, and the exhaust pipe first end is connected with the incubator aperture, and the exhaust pipe second end passes through described
Waste gas filter simultaneously from top to bottom immerses filtering liquid layer in it, and the oxygen storage device oxygen cathete is filled by aperture and the culture
It sets and is connected, the air blown producer is mounted on the culture chamber inner sidewall corresponding with the exhaust pipe first end, the electromagnetism
Valve is mounted on the culture chamber inner sidewall and is connected with the oxygen cathete, and the carbon dioxide sensor is mounted on the culturing rack
On, the circuit control device and the power supply are mounted on the incubator lateral wall, the motor base, the air blower,
The solenoid valve, the carbon dioxide sensor and the power supply are electrically connected with the circuit control device.
2. biotechnology cell culture apparatus according to claim 1, which is characterized in that the culture box material is to protect
Adiabator.
3. biotechnology cell culture apparatus according to claim 1, which is characterized in that the circuit control device is also
It include: temperature sensor and touch control display, the temperature sensor is mounted on the culturing rack, the touch control display peace
Mounted in the incubator one side wall, the temperature sensor and the touch control display are electric with the circuit control device ontology
Connection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910204106.6A CN109868221A (en) | 2019-03-18 | 2019-03-18 | A kind of biotechnology cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910204106.6A CN109868221A (en) | 2019-03-18 | 2019-03-18 | A kind of biotechnology cell culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109868221A true CN109868221A (en) | 2019-06-11 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910204106.6A Pending CN109868221A (en) | 2019-03-18 | 2019-03-18 | A kind of biotechnology cell culture apparatus |
Country Status (1)
| Country | Link |
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| CN (1) | CN109868221A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111670811A (en) * | 2020-07-31 | 2020-09-18 | 广西壮族自治区农业科学院 | Constant temperature device for cell tissue culture |
| CN111849773A (en) * | 2020-08-11 | 2020-10-30 | 中国工程物理研究院核物理与化学研究所 | Cell tritium water irradiation experimental device |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100056017A (en) * | 2008-11-19 | 2010-05-27 | 주식회사 지니스 | Incubator for cell culture |
| CN207175971U (en) * | 2017-07-31 | 2018-04-03 | 恒盛高科（天津）生物技术有限公司 | A kind of biotechnology cell culture apparatus |
| CN108707550A (en) * | 2018-06-20 | 2018-10-26 | 郑州源冉生物技术有限公司 | A kind of biotechnology cell culture apparatus |
| CN108795758A (en) * | 2018-06-26 | 2018-11-13 | 郑州源冉生物技术有限公司 | A kind of biotechnology research and development constant temperature cell culture apparatus |
-
2019
- 2019-03-18 CN CN201910204106.6A patent/CN109868221A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100056017A (en) * | 2008-11-19 | 2010-05-27 | 주식회사 지니스 | Incubator for cell culture |
| CN207175971U (en) * | 2017-07-31 | 2018-04-03 | 恒盛高科（天津）生物技术有限公司 | A kind of biotechnology cell culture apparatus |
| CN108707550A (en) * | 2018-06-20 | 2018-10-26 | 郑州源冉生物技术有限公司 | A kind of biotechnology cell culture apparatus |
| CN108795758A (en) * | 2018-06-26 | 2018-11-13 | 郑州源冉生物技术有限公司 | A kind of biotechnology research and development constant temperature cell culture apparatus |
Non-Patent Citations (1)
| Title |
|---|
| 王传政等: "二氧化碳培养箱使用要点及注意事项", 《医疗装备》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111670811A (en) * | 2020-07-31 | 2020-09-18 | 广西壮族自治区农业科学院 | Constant temperature device for cell tissue culture |
| CN111849773A (en) * | 2020-08-11 | 2020-10-30 | 中国工程物理研究院核物理与化学研究所 | Cell tritium water irradiation experimental device |
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| PB01 | Publication | ||
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| SE01 | Entry into force of request for substantive examination | ||
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190611 |
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| RJ01 | Rejection of invention patent application after publication |