CN109864244A - A kind of method that aurantiin improves beta carotene liposome stability - Google Patents
A kind of method that aurantiin improves beta carotene liposome stability Download PDFInfo
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- CN109864244A CN109864244A CN201711272435.1A CN201711272435A CN109864244A CN 109864244 A CN109864244 A CN 109864244A CN 201711272435 A CN201711272435 A CN 201711272435A CN 109864244 A CN109864244 A CN 109864244A
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Abstract
The invention belongs to food processing technology fields, disclose a kind of method that aurantiin improves beta carotene liposome stability, include the following steps: that beta carotene, lecithin, cholesterol are dissolved in 2: 1 chloroforms of volume ratio-methanol solution by (1), obtains the beta carotene solution that mass concentration is 0.1%~2.0%；(2) aurantiin is dissolved in methanol solution, and obtaining mass concentration is 0.5~10% aurantiin solution；(3) beta carotene solution and aurantiin solution are mixed under stirring condition, rotary evaporation removes organic solvent, and compound is made to form uniform film in round-bottomed flask bottom；(4) cholate solution will be added in complex thin film to redissolve, after sonicated, 3~6h is stirred at a temperature of 30~40 DEG C；Aurantiin-beta carotene liposome is obtained after mixed liquor centrifugation.The present invention prepares that beta carotene liposome stability is good, bioavailability is high, biological safety is good.
Description
Technical field
The invention belongs to functional food processing technique fields, and in particular to a kind of aurantiin raising beta carotene lipid
The method of body stability.
Background technique
Beta carotene is the pigment being widely present in red, orange and yellow fruits and vegetables, it not only has anticancer, antioxygen
Change, prevent the important physiological functions such as eye disease, and is the precursor substance that vitamin A synthesizes in humans and animals body.But β-carrot
The factors such as element is liposoluble substance, and dissolubility is poor in water, is not easy to disperse in body keep its bioavailability lower.In addition,
Unsaturated double-bond in its molecular structure is all unstable to light, oxygen and heat, limits beta carotene answering in medicine and food
With.
Emulsification system based on nanoemulsions and large biological molecule such as self-emulsifying carrier, nanometer self-emulsifying carrier, nanoparticle
And nanometer emulsified compound system embeds carotenoid, is to solve carotenoid dissolubility, stability and biology benefit
The effective way of expenditure.Liposome is one of the carrier system of field of food most application potential, because it is to slightly solubility nutrient
There is significant solubilization, and there is good biocompatibility, the absorption of mucous membrane, and oral lipid body are penetrated conducive to nutrient
Slow-releasing and controlled-releasing action is known as to nutrition, the absorption of nutrient can be extended.But liposome is as self assembly particle pharmaceutical dispersions, to acid,
The sensitivities such as heat easily assemble fusion, flocculation in storage period, core material are caused to leak.In addition, the line of content full cis-beta-carotene structure
Property degree and hardness increase the space occupy-place of molecule, make beta carotene that aggregation and crystallization easily occur in liposome, to lipid film
Structure have potential destruction.These factors adversely affect the stability of liposome, limit carotenoid rouge
Practical application of the plastid as function factor delivery vehicles.
Using covering material modified liposome, the internal external stability of liposome can be improved, delay active in liposome
Ingredient circulation time improves the bioavailability of nutrient.Flavonoid class substance can be adsorbed on the surface of fat drips, be grease cream
The good stabilizer of change system forms stable water-in-oil type pickering emulsion.Pickering emulsion is to utilize insoluble flavonoids
Oil-water interfaces are adsorbed in, form fine and close protective layer to prevent aggregation and the contraction of fat drips and make emulsion stability.In addition, by gallbladder
Salt is added in liposome the performance that lipoid plastid can be made to have height self-deformation, can efficiently pass through small duct, prepares
With high-effective penetrating, high-flexibility and hydrophilic liposome.Aurantiin is coated on β-carrot by self assembly by the present invention
Plain surface of liposome is aided with cholate and carries out secondary cladding to liposome, can be prepared a kind of with high stability, slow release effect
Beta carotene orient carrier.It will be helpful to improve external stability in beta carotene Via Liposomes, extend circulation time in vivo
And improve bioavilability.
Summary of the invention
The purpose of the present invention is to provide a kind of method that aurantiin improves beta carotene liposome stability, this method
The beta carotene liposome stability of preparation is good, bioavailability is high, biological safety is good.
For achieving the above object, the technical solution adopted by the present invention is that:
(1) beta carotene, lipid phase are dissolved in chloroform-methanol solution that volume ratio is 2: 1, obtain β-carrot
Plain lipoprotein solution.
(2) aurantiin is dissolved in methanol solution, obtains the aurantiin solution that mass concentration is 0.5~10%.
(3) beta carotene solution and aurantiin solution are mixed under stirring condition, rotary evaporation removes organic solvent, makes
Compound forms uniform film in round-bottomed flask bottom, improves vacuum degree and continues to drain 30min to 0.1MPa.
(4) cholate solution is added into step (3) complex thin film obtained to redissolve, ice-bath ultrasonic, ultrasonic power
800W continues 3~6h of stirring, 800~1200r/min of speed of agitator after handling 10~30min of time at a temperature of 30~40 DEG C；
(5) it will be centrifuged 10min under mixed liquor 5000r/min velocity conditions that step (4) obtains, obtains aurantiin-β-Hu
Radish element liposome.
Preferably, lipid phase described in step (1) is made of cholesterol and egg yolk lecithin.
Further, cholesterol and egg yolk lecithin total concentration are 2~8%, cholesterol and egg yolk lecithin mass ratio 0.2~
0.5。
Preferably, beta carotene concentration is 0.1~2.0% in beta carotene lipoprotein solution described in step (1).
Preferably, the volume ratio 1: 2~1: 10 of beta carotene solution described in step (3) and aurantiin solution.
Preferably, cholate concentration of polymer solution described in step (4) is 0.5~2%, pH 5.0~7.5.
Further, the molar ratio of cholate and lipid phase is 0.1~0.5.
The beneficial effects are mainly reflected as follows:
(1) present invention utilizes aurantiin modified liposome bilayer structure using cholesterol and egg yolk lecithin as membrane material
And performance, it is aided with the secondary cladding of cholate, constructs stable beta carotene liposome.Resulting liposome beta carotene embeds energy
Power is strong, and with the raising of carrying capacity, for beta carotene encapsulation rate up to 60%~80%, effective carrying capacity can be to 50%~80%.
(2) liposome stability is good, and average diameter is at 0.1~0.6 μM, and after storage in 10 days, average grain diameter increases ratio
Example is lower than 5%, shows that aurantiin cladding can effectively stable beta carotene liposome.This is because aurantiin is polyhydroxy
Macromolecular has molecular polarity, is adsorbed in oil-water interfaces, significantly enhances the activation energy that polar molecule passes through rouge body film, into
And effectively inhibit the infiltration of oxygen, hydrone etc. in storage period, there is good protective effect to beta carotene liposome.
(3) retention rate of liposome beta carotene prepared by the present invention is high, after storage in 10 days, carrying capacity 0.1%~
In 2.0% range, liposome beta carotene retention rate >=80%.
(4) present invention utilizes aurantiin coating beta-carotenoid lipid body, increases the polarity of liposome, is conducive to β-carrot
Element is transferred to digestive juice upper layer, significantly improves beta carotene liposome bioavailability.
(5) aurantiin of the present invention once coat, the secondary Coated Liposomes of cholate, improve beta carotene liposome in stomach and intestine
Stability in road.The liposome of preparation can inhibit β-carrot by that can resist gastric enzyme in gastrointestinal tract, acid and the destruction of bile
In the quick release stage of element, delays beta carotene liposome to discharge in the gastrointestinal tract, effectively improve beta carotene biological utilisation
Rate.
(6) in different load ranges, the present invention obtains liposome beta carotene bioavailability >=50%.
Detailed description of the invention
Fig. 1 beta carotene liposomal particle size distribution map
Specific embodiment
The following examples are a further detailed description of the invention, but are not meant to any limit of the invention
System.
Embodiment 1
Beta carotene, cholesterol and egg yolk lecithin are dissolved in chloroform-methanol solution that volume ratio is 2: 1,
It obtains containing 0.1% beta carotene, 1.0% egg yolk lecithin, the solution of 0.5% cholesterol.Aurantiin is dissolved in methanol solution
In, obtain the aurantiin solution that mass concentration is 0.5%.Beta carotene oil solution and aurantiin solution are mixed under stirring condition
It closes, rotary evaporation removes organic solvent, and compound is made to form uniform film in round-bottomed flask bottom.Complex thin film obtained
Middle addition pH7.4,0.75% cholate solution redissolve, ice-bath ultrasonic, ultrasonic power 800W, after being ultrasonically treated 15min, 37 DEG C of temperature
Under continue stir 4h, speed of agitator 1200r/min.It is centrifuged 10min under obtained mixed liquor 5000r/min velocity conditions, is obtained
Aurantiin-beta carotene liposome, as experimental group.
Control group: beta carotene, cholesterol and egg yolk lecithin are dissolved in chloroform-methanol that volume ratio is 2: 1
In solution, obtain containing 0.1% beta carotene, 1.0% egg yolk lecithin, the solution of 0.5% cholesterol.Rotary evaporation removing has
0.01mol/L is added in solvent, in pH7.4 phosphate buffer solution, ice-bath ultrasonic, and ultrasonic power 800W, ultrasonic treatment
After 15min, continue to stir 4h, speed of agitator 1200r/min at a temperature of 37 DEG C.Obtained mixed liquor 5000r/min velocity conditions
Lower centrifugation 10min obtains common beta carotene liposome, as a control group.
Detection: through detecting, control group beta carotene encapsulation rate is 65.7%, and effective carrying capacity is 55.5%, experimental group β-Hu
Radish element encapsulation rate is 66.1%, and effective carrying capacity is 52.3%, with control group without significant difference.Control group beta carotene liposome
0.15 μM of average grain diameter, after storage in 10 days, average grain diameter increases 20.4%, and experimental group beta carotene liposome is average
0.18 μM of partial size, after storage in 10 days, average grain diameter increases 2.4%.After storage in 10 days, control group beta carotene
Retention rate is 70.2%, and experimental group beta carotene retention rate is 85.2%.By control group and experimental group through in-vitro simulated stomach, intestines
Digestion experiment, result of study show release of the beta carotene of control group in gastric juice when carrying capacity beta carotene is 0.1%
Rate is higher than 10%, and the release rate in intestinal juice is higher than 30%, in entire digestion process, there is the beta carotene for accounting for total amount 30%
It is transferred in micellar phase.Release rate of the experimental group beta carotene in gastric juice is lower than 10%, and the release rate in intestinal juice is lower than
30%, in entire digestion process, there is the beta carotene for accounting for total amount 40% to be transferred in micellar phase.
Embodiment 2
Beta carotene, cholesterol and egg yolk lecithin are dissolved in chloroform-methanol solution that volume ratio is 2: 1,
It obtains containing 0.5% beta carotene, 2.0% egg yolk lecithin, the solution of 1.0% cholesterol.Aurantiin is dissolved in methanol solution
In, obtain the aurantiin solution that mass concentration is 2.0%.Beta carotene oil solution and aurantiin solution are mixed under stirring condition
It closes, rotary evaporation removes organic solvent, and compound is made to form uniform film in round-bottomed flask bottom.Complex thin film obtained
Middle addition pH6.8,1.5% cholate solution redissolve, ice-bath ultrasonic, ultrasonic power 800W, after being ultrasonically treated 20min, 37 DEG C of temperature
Under continue stir 5h, speed of agitator 1200r/min.It is centrifuged 10min under obtained mixed liquor 5000r/min velocity conditions, is obtained
Aurantiin-beta carotene liposome, as experimental group.
Control group: beta carotene, cholesterol and egg yolk lecithin are dissolved in chloroform-methanol that volume ratio is 2: 1
In solution, obtain containing 0.5% beta carotene, 2.0% egg yolk lecithin, the solution of 1.0% cholesterol.Rotary evaporation removing has
0.01mol/L is added in solvent, in pH7.4 phosphate buffer solution, ice-bath ultrasonic, and ultrasonic power 800W, ultrasonic treatment
After 20min, continue to stir 5h, speed of agitator 1200r/min at a temperature of 37 DEG C.Obtained mixed liquor 5000r/min velocity conditions
Lower centrifugation 10min obtains common beta carotene liposome, as a control group.
Detection: through detecting, control group beta carotene encapsulation rate is 72.1%, and effective carrying capacity is 70.2%, experimental group β-Hu
Radish element encapsulation rate is 73.5%, and effective carrying capacity is 69.5%, with control group without significant difference.Control group beta carotene liposome
0.23 μM of average grain diameter, after storage in 10 days, average grain diameter increases 25%, and experimental group beta carotene liposome is averaged grain
0.24 μM of diameter, after storage in 10 days, average grain diameter increases 3.7%.After storage in 10 days, control group beta carotene is protected
Staying rate is 65.4%, and experimental group beta carotene retention rate is 82.1%.Control group and experimental group are disappeared through in-vitro simulated stomach, intestines
Change experiment, result of study shows when carrying capacity beta carotene is 0.5%, release rate of the beta carotene of control group in gastric juice
Higher than 15%, the release rate in intestinal juice is higher than 35%, in entire digestion process, has the beta carotene for accounting for total amount 40% to turn
It moves in micellar phase.Release rate of the experimental group beta carotene in gastric juice is lower than 15%, and the release rate in intestinal juice is lower than
30%, in entire digestion process, there is the beta carotene for accounting for total amount 55% to be transferred in micellar phase.
Embodiment 3
Beta carotene, cholesterol and egg yolk lecithin are dissolved in chloroform-methanol solution that volume ratio is 2: 1,
It obtains containing 2.0% beta carotene, 3.0% egg yolk lecithin, the solution of 1.5% cholesterol.Aurantiin is dissolved in methanol solution
In, obtain the aurantiin solution that mass concentration is 4.0%.Beta carotene oil solution and aurantiin solution are mixed under stirring condition
It closes, rotary evaporation removes organic solvent, and compound is made to form uniform film in round-bottomed flask bottom.Complex thin film obtained
Middle addition pH6.0,2.25% cholate solution redissolve, ice-bath ultrasonic, ultrasonic power 800W, after being ultrasonically treated 20min, 37 DEG C of temperature
Under continue stir 5h, speed of agitator 1200r/min.It is centrifuged 10min under obtained mixed liquor 5000r/min velocity conditions, is obtained
Aurantiin-beta carotene liposome, as experimental group.
Control group: beta carotene, cholesterol and egg yolk lecithin are dissolved in chloroform-methanol that volume ratio is 2: 1
In solution, obtain containing 2.0% beta carotene, 3.0% egg yolk lecithin, the solution of 1.5% cholesterol.Rotary evaporation removing has
0.01mol/L is added in solvent, in pH6.0 phosphate buffer solution, ice-bath ultrasonic, and ultrasonic power 800W, ultrasonic treatment
After 20min, continue to stir 5h, speed of agitator 1200r/min at a temperature of 37 DEG C.Obtained mixed liquor 5000r/min velocity conditions
Lower centrifugation 10min obtains common beta carotene liposome, as a control group.
Detection: through detecting, control group beta carotene encapsulation rate is 78.4%, and effective carrying capacity is 80.5%, experimental group β-Hu
Radish element encapsulation rate is 76.5%, and effective carrying capacity is 79.8%, with control group without significant difference.Control group beta carotene liposome
0.32 μM of average grain diameter, after storage in 10 days, average grain diameter increases 31.4%, and experimental group beta carotene liposome is average
0.31 μM of partial size, after storage in 10 days, average grain diameter increases 3.0%.After storage in 10 days, control group beta carotene
Retention rate is 55.3%, and experimental group beta carotene retention rate is 87.4%.By control group and experimental group through in-vitro simulated stomach, intestines
Digestion experiment, result of study show carrying capacity beta carotene be 2% when, release rate of the beta carotene of control group in gastric juice
Higher than 20%, the release rate in intestinal juice is higher than 40%, in entire digestion process, has the beta carotene for accounting for total amount 50% to turn
It moves in micellar phase.Release rate of the experimental group beta carotene in gastric juice is lower than 20%, and the release rate in intestinal juice is lower than
40%, in entire digestion process, there is the beta carotene for accounting for total amount 70% to be transferred in micellar phase.
Claims (7)
1. a kind of method that aurantiin improves beta carotene liposome stability, it is characterised in that:
(1) beta carotene, lipid phase are dissolved in chloroform-methanol solution that volume ratio is 2: 1, obtain beta carotene rouge
Solution.
(2) aurantiin is dissolved in methanol solution, obtains the aurantiin solution that mass concentration is 0.5~10%.
(3) beta carotene lipoprotein solution and aurantiin solution are mixed under stirring condition, rotary evaporation removes organic solvent, makes multiple
It closes object and forms uniform film in round-bottomed flask bottom, improve vacuum degree and continue to drain 30min to 0.1MPa.
(4) cholate solution is added into step (3) complex thin film obtained to redissolve, ice-bath ultrasonic, ultrasonic power 800W, arteries and veins
5s/5s is rushed, after handling 10~30min of time, continues 3~6h of stirring, 800~1200r/ of speed of agitator at a temperature of 30~40 DEG C
min；
(5) it will be centrifuged 10min under mixed liquor 5000r/min velocity conditions that step (4) obtains, obtains aurantiin-beta carotene
Liposome.
2. the method that a kind of aurantiin according to claim 1 improves beta carotene liposome stability, feature exist
In: lipid phase is made of cholesterol and egg yolk lecithin.
3. the method that a kind of aurantiin according to claim 2 improves beta carotene liposome stability, feature exist
In: cholesterol is 2~8% with egg yolk lecithin total concentration, cholesterol and egg yolk lecithin mass ratio 0.2~0.5.
4. the method that a kind of aurantiin according to claim 1 improves beta carotene liposome stability, feature exist
In: beta carotene concentration is 0.1~2.0% in beta carotene lipoprotein solution.
5. the method that a kind of aurantiin according to claim 1 improves beta carotene liposome stability, feature exist
In: the volume ratio 1: 2~1: 10 of beta carotene solution and aurantiin solution.
6. the method that a kind of aurantiin according to claim 1 improves beta carotene liposome stability, feature exist
In: cholate concentration of polymer solution is 0.5~2%, pH5.0~7.5.
7. the method that a kind of aurantiin according to claim 1 improves beta carotene liposome stability, feature exist
In: the molar ratio of cholate and lipid phase is 0.1~0.5.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114747634A (en) * | 2022-05-10 | 2022-07-15 | 沈阳大学 | Preparation method of peanut milk rich in beta-carotene-loaded lipid particles |
| CN114947126A (en) * | 2020-12-15 | 2022-08-30 | 江苏省农业科学院 | Method for improving bioavailability of beta-carotene by modifying hesperidin |
| CN115530317A (en) * | 2022-11-03 | 2022-12-30 | 广东省农业科学院蚕业与农产品加工研究所 | Method for improving stability of mango peel carotenoid and stably embedding mango peel carotenoid |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030059462A1 (en) * | 2001-02-13 | 2003-03-27 | Yissum Research Development Company Of The Hebrew University | Carotenoid-loaded liposomes |
| CN104983591A (en) * | 2015-07-09 | 2015-10-21 | 西安艾尔菲生物科技有限公司 | Double modified beta-carotene lipidosome and preparation method thereof |
| CN107007553A (en) * | 2017-05-19 | 2017-08-04 | 泰山医学院 | A kind of polysaccharide cholate liposome for being used to be administered orally and preparation method thereof |
-
2017
- 2017-12-01 CN CN201711272435.1A patent/CN109864244B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030059462A1 (en) * | 2001-02-13 | 2003-03-27 | Yissum Research Development Company Of The Hebrew University | Carotenoid-loaded liposomes |
| CN104983591A (en) * | 2015-07-09 | 2015-10-21 | 西安艾尔菲生物科技有限公司 | Double modified beta-carotene lipidosome and preparation method thereof |
| CN107007553A (en) * | 2017-05-19 | 2017-08-04 | 泰山医学院 | A kind of polysaccharide cholate liposome for being used to be administered orally and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| ZIJUN LUO等: ""Particle-Stabilizing Effects of Flavonoids at the Oil-Water Interface"", 《JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114947126A (en) * | 2020-12-15 | 2022-08-30 | 江苏省农业科学院 | Method for improving bioavailability of beta-carotene by modifying hesperidin |
| CN114747634A (en) * | 2022-05-10 | 2022-07-15 | 沈阳大学 | Preparation method of peanut milk rich in beta-carotene-loaded lipid particles |
| CN115530317A (en) * | 2022-11-03 | 2022-12-30 | 广东省农业科学院蚕业与农产品加工研究所 | Method for improving stability of mango peel carotenoid and stably embedding mango peel carotenoid |
| CN115530317B (en) * | 2022-11-03 | 2023-08-22 | 广东省农业科学院蚕业与农产品加工研究所 | Method for improving stability and steady embedding of carotenoid in mango peel |
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