CN109856282A - The quick screening method of external source hazardous material in a kind of white wine - Google Patents
The quick screening method of external source hazardous material in a kind of white wine Download PDFInfo
- Publication number
- CN109856282A CN109856282A CN201910180137.2A CN201910180137A CN109856282A CN 109856282 A CN109856282 A CN 109856282A CN 201910180137 A CN201910180137 A CN 201910180137A CN 109856282 A CN109856282 A CN 109856282A
- Authority
- CN
- China
- Prior art keywords
- white wine
- hazardous material
- external source
- screening
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention discloses a kind of quick screening methods of external source hazardous material in white wine, the ethyl alcohol in white wine is removed using vacuum concentration first, freeze-dried reduction volume again, the concentration of HLB Solid Phase Extraction column purification, UPLC-Q-Tof detection, it is matched using the accurate mass number of unknown object compound characteristic quasi-molecular ions, daughter fragment ion information, isotope matching, retention time and white wine external source hazardous material database, the suspicious external source hazardous material of screening.Compared with prior art, Screening analysis white wine specificity of the present invention is strong, it is easy to operate, quick and precisely, analysis throughput it is high, pesticide residue, mycotoxin, sweetener, biogenic amine, bisphenol can be analyzed simultaneously, quickly analyze simultaneously and scientific method is provided for quality of white spirit control and external source hazardous material.
Description
Technical field
The present invention relates to technical field of analysis and detection, the rapid screening side of external source hazardous material in specifically a kind of white wine
Method.
Background technique
Pesticide is extremely important a part in agricultural modernization production, eliminates insect pest for crops, increases agricultural
Yield is most important.It is in addition native since Farming process needs the drugs such as herbicide spraying, insecticide, fungicide, auxin
Earth, water source may also cause pesticide to remain in grain by pollution by pesticides.Although the degradable part pesticide of fermentation,
It is that the organic matters such as the ethyl alcohol in white wine have good dissolubility to specific pesticide, leads to pesticide residue in wine, threaten consumption
The health and life security of person.Mycotoxin (Mycotoxin) is some tools that various fungies generate during the growth process
Virose secondary metabolite with extremely strong toxicity and has carcinogenic, teratogenesis, mutagenic effect.There is scholar from wine
Wine is with having isolated the aspergillus flavus with Toxin producing C in high temperature Daqu.Some scholars detect from yeast, fermented grain, yellow water etc.
Aflatoxin B l and ochratoxin A, but not can determine that above two mycotoxin in former wine and finished wine there are feelings
Condition.Therefore the non-targeted quick screening method for establishing unknown mycotoxin in white wine is very urgent and necessary.
In addition to this, sweetener can be also introduced in white wine, which is must not provide to detect in the existing Chinese sprits standard of country
The illegal additive of white wine, but still have Some Enterprises or it is personal add privately, damage consumer's interests.Biogenic amine is a kind of nitrogenous
Aliphatic (putrescine, cadaverine, spermine, spermidine), aromatic series (tyrasamine, phenyl ethylamine) or heterocyclic (histamine, tryptamines) low molecule
Compound is mainly formed by the amination and transamination of the de- shuttle of amino acid or aldehyde and ketone.Research shows that excessive take the photograph
The bad physiological reaction such as headache, abdominal cramps, vomiting can be caused by entering biogenic amine.It has been proved that the biogenic amine contained in grape wine
Up to 20 kinds, also there is part detection in white wine.Bisphenol-A (BisphenolA, BPA) is a kind of environmental classes endocrine disruptor,
Belong to hypotoxicity chemicals, animal experiment discovery bisphenol-A has the effect of simulation estrogen, animal can be made to produce very low dosage
The effects of raw female sex premature, sperm count decline, prostate increase.In addition, there is data to show that bisphenol-A has certain embryotoxicity
And teratogenesis, the generation of the cancers such as animal ovary cancer, prostate cancer, leukaemia can be obviously increased.It is present in various coatings, coating
In, it is thus possible to white wine can be introduced by wine storage tank, cause food-safety problem.
Above-mentioned five major class food-safety problem is the exogenous hazardous material that may be introduced in white wine, can individually be examined,
But time-consuming, and pre-treatment is time-consuming and laborious, causes greatly to perplex to enterprise.Therefore a kind of exogenous hazardous material for white wine is researched and developed
Screening method is very urgent.
With generally answering for the technologies such as gas chromatography-mass spectrography (GC-MS), liquid chromatograph mass spectrography (LC-MS)
It is rapidly developed with the detection technique of, pesticide multi-residues, mycotoxin.It is with quadrupole rod series connection flight mass spectrum (Q-Tof)
The advantages that high resolution mass spectrum of representative has high resolution, and accurate mass number measures, highly sensitive under full scan, can be to complicated base
Compound in matter carries out qualitative confirmation.Since Q-Tof has high scanning speed, can not have to consider the limit on compound amounts
System realizes the screening high-throughput simultaneously of a large amount of pesticides.It can also be strong by the accurate mass number of compound and retention time, isotope
The information such as degree, isotope distribution, secondary fragment combine, and realize the quick without standard items of pesticide with specific compound database matching
Screening.Therefore, Q-Tof is widely used in the safety detection of varieties of food items.But the matrix of Wine Sample is complicated, contains
2000 lot of trace ingredients, not only can jamming target object (agricultural chemical compound) analysis, but also chromatographic column and mass spectrum can be caused
Fatal damage, therefore how effectively extracting object and purifying the interference impurity in removal white wine becomes a difficulty of urgent need to resolve
Topic.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of quick screening methods of external source hazardous material in white wine.This
Inventive method Screening analysis white wine specificity is strong, it is easy to operate, quick and precisely, analysis throughput it is high, can analyze simultaneously pesticide residue,
Mycotoxin, sweetener, biogenic amine, bisphenol are quickly analyzed provide simultaneously for quality of white spirit control and external source hazardous material
Scientific method.
The quick screening method of external source hazardous material, includes the following steps: in white wine of the present invention
Step 1: sample pre-treatments
Take white wine sample 500mL in evaporative flask, 40 DEG C of evaporating temperature, revolving speed 120r/min, concentrated by rotary evaporation to 200mL,
Taking-up is placed on drying in vacuum freeze drier and is condensed into solid, and 5mL ultrapure water is then added and redissolves, separately takes 5mL in three times
Ware wall is rinsed, 10mL concentrate is merged；The activation of 3mL methanol is taken to balance pillar in HLB solid-phase extraction column, then with 3mL ultrapure water, it will
10mL concentrate continues through the solid-phase extraction column of activation with the speed of 1mL/min, is then eluted with 2mL deionized water, takes out true
The dry solid-phase extraction column 5min of sky, discards efflux, finally elutes solid-phase extraction column with 5mL methanol, collect eluent, 40 DEG C of water
Nitrogen drying is bathed, 10% acetonitrile water is settled to 1mL, as prepare liquid；
Step 2:UPLC-Q-Tof detection
Prepare liquid divides the external sources hazardous material such as pesticide residue and mycotoxin by ultra performance liquid chromatography system
From, using be furnished with electric spray ion source UPLC-Q-Tof, MS is respectively adoptedEES+ and ES- both of which be acquired；
Step 3: external source hazardous material screening
Using MSEMode acquires sample parent ion and daughter ion data information simultaneously, in conjunction with the exogenous hazardous material number of white wine
Screening is carried out according to library, scientific library is according to accurate parent ion quality, retention time, fragment ion and isotope model
And intensity is identified, the information of unknown component m/z is obtained.
In step 2, the detection parameters of UPLC-Q-Tof detection are as follows:
Chromatographic condition:
Chromatographic column: ACQUITY UPLC Ben C18Column, 2.1mm × 50mm, 1.7 μm；
Mobile phase: mobile phase A is 0.1% formic acid water, and Mobile phase B is acetonitrile, flow velocity: 0.6mL/min, gradient elution；
Sample volume: 2 μ L；
Column temperature: 35 DEG C；
Mass Spectrometry Conditions:G2-S QTof mass spectrograph；Acquisition mode: MSE mode；
ESI+ ionizes mode condition: capillary voltage: 3.0kV；Sample orifice potential: 40V；Ion source temperature: 120 DEG C；
Desolventizing temperature: 450 DEG C；Desolvation gas flow velocity: 1000L/H；Reference mass: leucine enkephalin [M+H] +=
556.2771。
ESI- ionizes mode condition: capillary voltage: 2.5kV；Sample orifice potential: 40V；Ion source temperature: 120 DEG C；
Desolventizing temperature: 400 DEG C；Desolvation gas flow velocity: 1000L/H；Reference mass: leucine enkephalin [M-H] -=
554.2615。
Acquisition range: m/z 50~1200；Low energy collisions energy: 4eV；High energy gradual change collision energy: 10~45eV.
In step 2, gradient elution program is provided that
0~1min:90%A+10%B；1~4.5min:55%A+45%B；4.5min~4.6min:45%A+55%B；
4.6~7.9min:10%A+90%B；7.9~8.0min:10%A+90%B；8.0~8.1min:90%A+10%B；8.1
~10min:90%A+10%B.
In step 3, retrieval parameter are as follows: retention time range of search is ± 0.01min, and accurate mass deviation is 2mDa, from
Sonization formal character+H and-H mode.
Compared with prior art, the beneficial effects of the present invention are embodied in:
The present invention uses rotary evaporation-freeze-drying-HLB- nitrogen to blow for pretreatment mode, returns to the exogenous hazardous material of white wine
High income, stability are good, can effectively remove interfering substance, while quickly qualitative without standard items to realizing using UPLC-Q-Tof
Screening.This method quick and precisely, analysis throughput it is high, specificity is strong, analysis type is abundant, can be quality of white spirit control and external source danger
Quickly analysis provides scientific method to evil object simultaneously.
Detailed description of the invention
Fig. 1 is malathion (Malathion), chlopyrifos (Chlorpyrifos), zearalenone
(Zearalenone), chromatography of ions figure is extracted under histamine (Histamine) ES+ mode；
Fig. 2 is to extract ion chromatography under neotame (Neotame), bisphenol S (4,4'-Sulfonyldiphenol) ES- mode
Figure；
Fig. 3 is white wine external source hazardous material rapid screening verification result thumbnail ES+；
Fig. 4 is white wine external source hazardous material rapid screening verification result thumbnail ES-.
Specific embodiment
Of the invention for ease of understanding, below with reference to specific implementation example, the invention will be further described.
Embodiment:
1.1 reagents, drug
Pesticide residue, mycotoxin, sweetener, biogenic amine, bisphenol S standard substance, it is single to mark stock solution, by 10% acetonitrile
Water configuration, -20 DEG C are kept in dark place.
1.2 instrument and equipment
Waters ACQUITYI-Class systemG2-XS QTof mass spectrograph is furnished with electron spray ion
Source (ESI)；Switzerland walks fine jade R-210 vacuum and rotates evaporimeter；U.S.'s LABCONCO 4.5L vacuum freeze drier；CNW Poly-
Sery HLB solid-phase extraction column；U.S.'s Organomation N-EVAP-112 nitrogen evaporator.
1.3 sample pre-treatments
It takes white wine 500mL in evaporative flask, 40 DEG C of evaporating temperature, revolving speed 120r/min, is concentrated by evaporation to 200mL and takes out.
It is contained in evaporating dish and is condensed into solid in vacuum freeze drier, 5mL ultrapure water is added and redissolves, separately 5mL is taken to rinse ware in three times
Wall merges 10mL concentrate.The activation of 3mL methanol is taken to balance pillar in HLB solid phase extraction column, then with 3mL ultrapure water.10mL is dense
Contracting liquid continues through the solid-phase extraction column of activation with the speed of 1mL/min, after sample all exhausts, is eluted with 2mL deionized water
Pillar, vacuum drying solid phase extraction column 5min, discards efflux.Pillar finally is eluted with 5mL methanol, collects eluent,
40 DEG C of water-bath nitrogen dryings, it is to be measured that 10% acetonitrile water is settled to 1mL.
1.4 UPLC-Q-Tof detection
Compound is separated by liquid chromatographic system, chromatographic condition: chromatographic column: ACQUITY UPLC Ben C18
Column, 2.1mm × 50mm, 1.7 μm；Mobile phase: 0.1% formic acid water of A phase, B phase are acetonitrile, flow velocity: 0.6mL/min, gradient
Elution；0~1min:90%A+10%B；1~4.5min:55%A+45%B；4.5min~4.6min:45%A+55%B；
4.6~7.9min:10%A+90%B；7.9~8.0min:10%A+90%B；8.0~8.1min:90%A+10%B；8.1~
10min:90%A+10%B.Sample volume: 2 μ L；Column temperature: 35 DEG C；Mass Spectrometry Conditions:G2-S QTof mass spectrograph acquires mould
Formula: MSE mode；ESI+ ionizes mode condition: capillary voltage: 3.0kV；Sample orifice potential: 40V；Ion source temperature: 120
℃；Desolventizing temperature: 450 DEG C；Desolvation gas flow velocity: 1000L/H；Reference mass: leucine enkephalin [M+H] +=
556.2771.ESI- ionizes mode condition: capillary voltage: 2.5kV；Sample orifice potential: 40V；Ion source temperature: 120 DEG C；
Desolventizing temperature: 400 DEG C；Desolvation gas flow velocity: 1000L/H；Reference mass: leucine enkephalin [M-H] -=
554.2615.Acquisition range: m/z 50~1200；Low energy collisions energy: 4eV；High energy gradual change collision energy: 10~45eV.
1.5 screening method
Using MSEMode acquires parent ion and daughter ion data information under ES+ and ES- ionization mode, respectively to be free of
The residual ultrapure water of agriculture carries out screening using white wine external source hazardous material database as control, in conjunction with UNFI software, according to accurate
Parent ion quality, retention time, fragment ion and isotope model and intensity identified, rapid evaluation unknown component m/z
Information.
2. results and discussion
The rate of recovery and stability of 2.1 sample-pretreating methods
The matrix of Wine Sample is complicated, contains 2,000 lot of trace ingredients, not only can jamming target object it is (pesticide residue, true
Verticillium toxin) analysis, and fatal damage can be caused to chromatographic column and mass spectrum, therefore how effectively extract object and purify
Removing the interference impurity in white wine becomes a problem of urgent need to resolve.This experiment is first removed in white wine using vacuum concentration
Most ethyl alcohol, the reduction volume of vacuum freeze drying high degree, then extracted by CNW Poly-Sery HLB SPE solid phase
Take column purification to extract, then the accuracy and repeatability of this method evaluated, in pesticide residue malathion, poison with poison
Tick, zearalenone, the histamine in biogenic amine, the neotame in sweetener, the bisphenol S in bisphenol in mycotoxin
For, appearance time be respectively 3.96min, 4.95min, 3.59min, 0.32min, 2.28min, 2.81min, see Fig. 1 and
Fig. 2, under 200,300 and 500ng/mL pitch-based sphere, the rate of recovery is in 85%~108% range, and RSD < 10% (n=
5).Experimental result illustrates that the accuracy of pre-treating method and repeatability are good, can be applied to external source hazardous material in white wine and quickly sieves
It looks into.
The optimization of 2.2 detection parameters
This method screening use without agriculture is residual and the ultrapure water of mycotoxin as a control group, can remove significant false positive knot
It is ± 0.01min that fruit, which further includes retention time range of search, and accurate mass deviation is -2mDa~2mDa, ionized form selection
+ H and-H mode.
External source hazardous material rapid screening verification method in 2.3 white wine
Choose 1,4-DIAMINOBUTANE, 4,4'-Sulfonyldiphenol, AFLATOXIN B1, AFLATOXIN
B2、 AFLATOXIN G2、AFLATOXIN M1、Alitame、ALTENUENE、alternariol、alternariol
monomethyl ether、Aspartame、BETA-HCH、Bisphenol A、Chlorpyrifos、Chlorpyrifos-
methyl、 CYCLOPIAZONIC ACID_1、CYCLOPIAZONIC ACID_1、FUSARIC ACID、Histamine、 HT-
2TOXIN、Malathion、Neotame、OCHRATOXIN A、PAXILLINE、Spermidine、Spermine、
Stevioside、Zearalenone、OCHRATOXIN A、Sucralose、P,P'-DDD、enniatin B1、enniatin A
Deng 33 kinds of exogenous hazardous materials of white wine, 500mL is added to without in the white wine of above compound with the content of 500ng/mL, is pressed
Experiment and screening are carried out according to 1.3~1.5, screening results are shown in Fig. 3 (ES+ mode) and Fig. 4 (ES- mode), test screening results
It see the table below.
The analysis of 2.5 screening results
As seen from the above table, the exogenous hazardous material of 33 kinds of white wine all detects, recall rate 100%, and each chemical combination material resource implosion
Skill is found, and extracts ion chromatography peak at symmetrical peak, as a result reliably.Show UPLC-Q- used by institute of the invention simultaneously
Tof system and related white wine external source hazardous material database, screening software, retention time locking, the matching of accurate mass number, son
The technologies such as fragment ion matching, isotope matching, can accurately and reliably realize the screening of external source hazardous material in white wine.
Claims (5)
1. the quick screening method of external source hazardous material in a kind of white wine, it is characterised in that include the following steps:
Step 1: sample pre-treatments
White wine is rotated to vacuum freeze drying after removing alcohol, then through HLB Solid Phase Extraction column purification, 40 DEG C of nitrogen are dried up, 10%
Acetonitrile water is settled to 1mL, as prepare liquid；
Step 2:UPLC-Q-Tof detection
Prepare liquid separates the external sources hazardous material such as pesticide residue and mycotoxin by ultra performance liquid chromatography system, adopts
With the UPLC-Q-Tof for being furnished with electric spray ion source, MS is respectively adoptedEES+ and ES- both of which be acquired；
Step 3: external source hazardous material screening
Using MSEMode acquires sample parent ion and daughter ion data information simultaneously, in conjunction with the exogenous hazardous material database of white wine into
Row screening, scientific library is according to accurate parent ion quality, retention time, fragment ion and isotope model and intensity
It is identified, obtains the information of unknown component m/z.
2. according to the method described in claim 1, it is characterized by:
Step 1 includes the following steps:
Take white wine sample 500mL in evaporative flask, 40 DEG C of evaporating temperature, revolving speed 120r/min, concentrated by rotary evaporation to 200mL is taken out
It is placed on drying in vacuum freeze drier and is condensed into solid, 5mL ultrapure water is then added and redissolves, separately 5mL is taken to rinse in three times
Ware wall merges 10mL concentrate；The activation of 3mL methanol is taken to balance pillar in HLB solid-phase extraction column, then with 3mL ultrapure water, by 10mL
Concentrate continues through the solid-phase extraction column of activation with the speed of 1mL/min, is then eluted, is vacuumized dry with 2mL deionized water
Dry solid-phase extraction column 5min, discards efflux, finally elutes solid-phase extraction column with 5mL methanol, collects eluent, 40 DEG C of water-bath nitrogen
Drying, 10% acetonitrile water are settled to 1mL, as prepare liquid.
3. according to the method described in claim 1, it is characterized by:
In step 2, the detection parameters of UPLC-Q-Tof detection are as follows:
Chromatographic condition:
Chromatographic column: ACQUITY UPLC Ben C18 Column, 2.1mm × 50mm, 1.7 μm；
Mobile phase: mobile phase A is 0.1% formic acid water, and Mobile phase B is acetonitrile, flow velocity: 0.6mL/min, gradient elution；
Sample volume: 2 μ L；
Column temperature: 35 DEG C；
Mass Spectrometry Conditions:G2-S QTof mass spectrograph；Acquisition mode: MSEMode；
ESI+ ionizes mode condition: capillary voltage: 3.0kV；Sample orifice potential: 40V；Ion source temperature: 120 DEG C；Precipitation
Agent temperature: 450 DEG C；Desolvation gas flow velocity: 1000L/H；Reference mass: leucine enkephalin [M+H] +=556.2771.
ESI- ionizes mode condition: capillary voltage: 2.5kV；Sample orifice potential: 40V；Ion source temperature: 120 DEG C；Precipitation
Agent temperature: 400 DEG C；Desolvation gas flow velocity: 1000L/H；Reference mass: leucine enkephalin [M-H] -=554.2615.
Acquisition range: m/z 50~1200；Low energy collisions energy: 4eV；High energy gradual change collision energy: 10~45eV.
4. according to the method described in claim 3, it is characterized by:
In step 2, gradient elution program is provided that
0~1min:90%A+10%B；1~4.5min:55%A+45%B；4.5min~4.6min:45%A+55%B；4.6
~7.9min:10%A+90%B；7.9~8.0min:10%A+90%B；8.0~8.1min:90%A+10%B；8.1~
10min:90%A+10%B.
5. according to the method described in claim 1, it is characterized by:
In step 3, retrieval parameter are as follows: retention time range of search is ± 0.01min, and accurate mass deviation is 2mDa, ionization
Formal character+H and-H mode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910180137.2A CN109856282A (en) | 2019-03-11 | 2019-03-11 | The quick screening method of external source hazardous material in a kind of white wine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910180137.2A CN109856282A (en) | 2019-03-11 | 2019-03-11 | The quick screening method of external source hazardous material in a kind of white wine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109856282A true CN109856282A (en) | 2019-06-07 |
Family
ID=66900319
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910180137.2A Pending CN109856282A (en) | 2019-03-11 | 2019-03-11 | The quick screening method of external source hazardous material in a kind of white wine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109856282A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113092651A (en) * | 2021-03-29 | 2021-07-09 | 广州广电计量检测股份有限公司 | Method for detecting sweetener in milk or dairy product |
| CN113495107A (en) * | 2021-06-17 | 2021-10-12 | 河北省农林科学院遗传生理研究所（河北省农林科学院农产品质量安全研究中心） | Method for simultaneously detecting residual quantity of mycotoxin and pesticide in fruits |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101634648A (en) * | 2009-08-21 | 2010-01-27 | 江南大学 | Method for detecting six trace sweetening agents in white spirit by ultra-high pressure liquid chromatography and time-of-flight mass spectrometry |
| CN106324150A (en) * | 2016-11-25 | 2017-01-11 | 四川省食品药品检验检测院 | Method for determining eight sweetening agents in Baijiu by high-performance liquid chromatography-low-temperature evaporative light scattering detector method |
| CN107478737A (en) * | 2017-07-18 | 2017-12-15 | 贵州茅台酒股份有限公司 | A kind of method of 80 kinds of persticide residues in detection white wine |
| CN108490098A (en) * | 2018-05-21 | 2018-09-04 | 安徽瑞思威尔科技有限公司 | The non-targeted quick screening method of unknown pesticide residue in a kind of white wine |
| CN108956801A (en) * | 2018-05-21 | 2018-12-07 | 安徽瑞思威尔科技有限公司 | The non-targeted quick screening method of unknown mycotoxin in a kind of white wine |
-
2019
- 2019-03-11 CN CN201910180137.2A patent/CN109856282A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101634648A (en) * | 2009-08-21 | 2010-01-27 | 江南大学 | Method for detecting six trace sweetening agents in white spirit by ultra-high pressure liquid chromatography and time-of-flight mass spectrometry |
| CN106324150A (en) * | 2016-11-25 | 2017-01-11 | 四川省食品药品检验检测院 | Method for determining eight sweetening agents in Baijiu by high-performance liquid chromatography-low-temperature evaporative light scattering detector method |
| CN107478737A (en) * | 2017-07-18 | 2017-12-15 | 贵州茅台酒股份有限公司 | A kind of method of 80 kinds of persticide residues in detection white wine |
| CN108490098A (en) * | 2018-05-21 | 2018-09-04 | 安徽瑞思威尔科技有限公司 | The non-targeted quick screening method of unknown pesticide residue in a kind of white wine |
| CN108956801A (en) * | 2018-05-21 | 2018-12-07 | 安徽瑞思威尔科技有限公司 | The non-targeted quick screening method of unknown mycotoxin in a kind of white wine |
Non-Patent Citations (3)
| Title |
|---|
| KAI ZHANG ET AL.: "Multiresidue Pesticide Analysis of Wines by Dispersive Solid-Phase Extraction and Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry", 《JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY》 * |
| 李似姣等: "《现代色谱分析》", 30 June 2014, 国防工业出版社 * |
| 赵玉玲等: "超高效液相色谱-三重四级杆质谱法同时检测葡萄酒中16种高关注度化合物", 《食品安全质量检测学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113092651A (en) * | 2021-03-29 | 2021-07-09 | 广州广电计量检测股份有限公司 | Method for detecting sweetener in milk or dairy product |
| CN113495107A (en) * | 2021-06-17 | 2021-10-12 | 河北省农林科学院遗传生理研究所（河北省农林科学院农产品质量安全研究中心） | Method for simultaneously detecting residual quantity of mycotoxin and pesticide in fruits |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Walorczyk | Validation and use of a QuEChERS-based gas chromatographic–tandem mass spectrometric method for multiresidue pesticide analysis in blackcurrants including studies of matrix effects and estimation of measurement uncertainty | |
| Esquenazi et al. | Imaging mass spectrometry of natural products | |
| CN106053703B (en) | It is a kind of at the same detect food in various plants growth regulator method | |
| Greer et al. | Mass spectrometry imaging for drugs and metabolites | |
| Strege | High-performance liquid chromatographic–electrospray ionization mass spectrometric analyses for the integration of natural products with modern high-throughput screening | |
| Frenich et al. | Monitoring multi-class pesticide residues in fresh fruits and vegetables by liquid chromatography with tandem mass spectrometry | |
| Hernández et al. | Multiresidue liquid chromatography tandem mass spectrometry determination of 52 non gas chromatography-amenable pesticides and metabolites in different food commodities | |
| Chipuk et al. | Transmission mode desorption electrospray ionization | |
| Van Berkel et al. | Established and emerging atmospheric pressure surface sampling/ionization techniques for mass spectrometry | |
| Fernandez et al. | Liquid chromatographic–mass spectrometric determination of post-harvest fungicides in citrus fruits | |
| Agüera et al. | One-year routine application of a new method based on liquid chromatography–tandem mass spectrometry to the analysis of 16 multiclass pesticides in vegetable samples | |
| Kauppila et al. | Rapid analysis of metabolites and drugs of abuse from urine samples by desorption electrospray ionization-mass spectrometry | |
| Pozo et al. | Determination of the herbicide 4-chloro-2-methylphenoxyacetic acid and its main metabolite, 4-chloro-2-methylphenol in water and soil by liquid chromatography–electrospray tandem mass spectrometry | |
| Ramasubramanian et al. | A simple and sensitive single-step method for gas chromatography–mass spectrometric determination of fipronil and its metabolites in sugarcane juice, jaggery and sugar | |
| Yang et al. | Simultaneous analysis of multiple pesticide residues in minor fruits by ultrahigh-performance liquid chromatography/hybrid quadrupole time-of-fight mass spectrometry | |
| Lee et al. | Multiresidue pesticide analysis in Korean ginseng by gas chromatography–triple quadrupole tandem mass spectrometry | |
| Avula et al. | Identification and characterization of indole and oxindole alkaloids from leaves of Mitragyna speciosa Korth using liquid chromatography–accurate QToF mass spectrometry | |
| CN109856282A (en) | The quick screening method of external source hazardous material in a kind of white wine | |
| Ozawa et al. | Direct imaging mass spectrometry of plant leaves using surface-assisted laser desorption/ionization with sputterdeposited platinum film | |
| Caixach et al. | Liquid chromatography–mass spectrometry | |
| Picó et al. | Rapid and sensitive ultra-high-pressure liquid chromatography–quadrupole time-of-flight mass spectrometry for the quantification of amitraz and identification of its degradation products in fruits | |
| CN108490098A (en) | The non-targeted quick screening method of unknown pesticide residue in a kind of white wine | |
| Dopstadt et al. | Localization of ergot alkaloids in sclerotia of Claviceps purpurea by matrix-assisted laser desorption/ionization mass spectrometry imaging | |
| US10697945B2 (en) | Method of analyzing compound including amine group by using Boc compound | |
| El Atrache et al. | Identification of phenyl-N-methylcarbamates and their transformation products in Tunisian surface water by solid-phase extraction liquid chromatography–tandem mass spectrometry |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190607 |