CN109852705A - Horse derived components rapid detection method and kit in a kind of food - Google Patents
Horse derived components rapid detection method and kit in a kind of food Download PDFInfo
- Publication number
- CN109852705A CN109852705A CN201910290268.6A CN201910290268A CN109852705A CN 109852705 A CN109852705 A CN 109852705A CN 201910290268 A CN201910290268 A CN 201910290268A CN 109852705 A CN109852705 A CN 109852705A
- Authority
- CN
- China
- Prior art keywords
- horse
- lamp
- detection
- gene
- derived components
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention provides horse derived components quick detection kit in a kind of food, is related to biological species identification technology field.The present invention filters out the general internal standard gene LOC106782588 in a horse source first, its nucleotide sequence is located on the 25th chromosome, copy number is constant in horse species as shown in SEQ ID NO.1, there is no allelic variations, can be used as the target gene of identification Ma Yuan.LAMP amplimer is devised by target sequence of the gene, constant-temperature amplification is carried out together with LAMP reaction solution, LAMP reaction product is detected for colloidal gold nucleic acid test strip.The detection of LAMP reaction bonded colloidal gold nucleic acid test strip constitutes quick detection kit, can rapidly and sensitively detect the horse derived components in food, detection sensitivity is up to 0.2% (w/w).Kit application method of the present invention is simple, low in cost, and reaction result is easy to observe, and specificity is good, is highly suitable for live real-time detection.
Description
Technical field
The present invention relates to biological species identification technology fields, more particularly to a kind of ring of horse derived components in detection food
The quick detection kit of mediated isothermal amplification technology (LAMP) association colloid gold nucleic acid test strip detection.
Background technique
With rapid development of economy, the raising of living standards of the people, demand of China resident to meat product increases year by year
Add.Although many country's clear stipulaties mark type, the source of meat with requiring food labelling true, unambiguous, forbid adulterated behavior,
But have the event of many meat adulterations, such as still in the market in order to reduce cost, be mixed in donkey fire pork or
Horseflesh mixes beef in mutton or other meats etc. behavior is adulterated by pork, Malaysia and China.Currently, for the detection side of food adulteration
Method is mainly PCR method.PCR method is needed by specific apparatus, and the judgement of final result needs agarose gel electrophoresis complete
At whole process takes a long time.Therefore, it is an object of the present invention to provide a kind of rapid sensitive detection reagents of horse derived components in food
Box.
Currently, internal standard gene is widely used for identifying food adulteration, but how to filter out suitable internal standard base
Because being particularly important.Current meat products detection of adulterations technology both domestic and external is specifically drawn for the gene design on mitochondria
Object carries out real-time fluorescence quantitative PCR amplification, and since chondriogen is multi-copy gene, detection sensitivity is high, but simultaneously
There is puzzlement when distinguishing as unconscious cross contamination caused by processes and the conscious illegal addition such as selling, transporting.
In addition, the concentration of high copy number mitochondrial DNA can not be corresponding with the concentration of genomic DNA, therefore essence can not be carried out to sample
True quantitative analysis, simultaneously because chondriogen homology is high, it is difficult to realize qualitative detection by regular-PCR.Therefore, the party
Method can only realize that the screening of meat adulteration identifies by quantitative fluorescent PCR.To identify the cross contamination unintentionally of low concentration and having
The illegal addition of meaning and clearly adulterated ratio realization rapid screening, then want the low copy gene on selective staining body as meat internal standard
Quasi- gene.
Loop-mediated isothermal amplification (loop-mediated isotherm amplification, LAMP) be by
Constant temperature nucleic acid amplification method Notomi novel in one kind of exploitation in 2000, principle are polymerize using a kind of strand displacement DNA
Enzyme (BstDNA polymerase) and two pairs of special primers specifically identify 6 isolated areas on target sequence, in isothermal
Under the conditions of (65 DEG C or so) heat preservation dozens of minutes, nucleic acid amplification reaction can be completed.
Colloidal gold nucleic acid test strip is the specific binding by antigen and antibody, therefore it is with high sensitivity.
The present invention combines LAMP technology with the detection of colloidal gold nucleic acid test strip, provides a kind of quick spirit of horse derived components in food
Quick detection kit.The present invention is without complicated instrument, and the time is short, and easy to operate, high sensitivity can satisfy live inspection completely
The demand of survey.
Summary of the invention
The object of the present invention is to provide the general internal standard genes in horse source for detecting horse derived components in food.
Another object of the present invention is to provide a kind of in high sensitivity, high specific, detection food easy to operate
The quick detection kit of the LAMP association colloid gold nucleic acid test strip detection of horse derived components.
It is a kind of for detecting the gene of horse derived components in food, be internal standard gene LOC106782588, there is SEQ
Sequence shown in ID NO.1.
The present invention provides application of the above-mentioned internal standard gene LOC106782588 in detection horse derived components.
The application that the present invention provides above-mentioned internal standard gene LOC106782588 in food in the identification of horse derived components.
The present invention provides a kind of for detecting the specific LAMP primer group of above-mentioned internal standard gene LOC106782588
It closes, including following 4 primers:
F3:5 '-TGGATCTGAGGAGATGAGG-3 '；
B3:5 '-TTGAGGAGCATAGTCTTGAA-3 '；
FIP:5 '-ATAGCTCCATCAGATCCTGGGCCAGGGTGCAGTAAAAGC-3 '；
BIP:5 '-CAAGGGAAGGTAGAGTCAGAGGGGAGGATGTCAGTATGAGAG-3 '.
5 ' end mark fluorescents of wherein 5 ' end labels biotin (Biotin) of inner primer FIP, BIP are plain (FITC).
The present invention provides application of the above-mentioned specific LAMP primer composition in the identification of horse derived components.
The present invention provides above-mentioned specific LAMP primer compositions in preparation horse derived components detection kit or detection reagent
In application.
Further, the present invention provides a kind of the quick of detection horse derived components containing above-mentioned specific LAMP primer composition
Detection kit.
The present invention provides a kind of method for detecting horse derived components in food, comprising the following steps:
(1) sample to be tested extracts DNA；
(2) to extract DNA as template, LAMP detection is carried out using specificity LAMP primer composition described in claim 3；
(3) result judges: carrying out result judgement using colloidal gold nucleic acid test strip.Detection T line and Quality Control C line have red
Band contains target gene；Quality Control C line has red stripes, and detects T line without band, does not contain target gene.
In the above method, step (2) the LAMP detection, the concrete configuration of 25 μ L LAMP detection architectures are as follows: 1 ×
Thermopol buffer, 0.4mM dNTP, 3mM MgSO4, 1.0M glycine betaine, 1.6 μM of primers Fs IP, 1.6 μM of primer BIP,
0.2 μM of primers F, 3,0.2 μM of primer B3,8U Bst archaeal dna polymerase large fragment.
In the above method, LAMP detects reaction condition are as follows: then 60-65 DEG C of constant temperature 20min, 85 DEG C of 5min terminate reaction.
In the above method, colloidal gold nucleic acid test strip described in step (3) include colloidal gold nucleic acid test strip p-wire with
Nature controlling line, p-wire are marked with FITC antibody, and nature controlling line is marked with biotin secondary antibody, in conjunction with being lined with colloidal gold-biotin antibody
Marker.
The present invention screens source internal standard gene of going into action on chromosome for the first time.The present invention verifies internal standard with multiple kind horses
Gene, it was demonstrated that the internal standard gene is stablized, and allelic variation is not present.The selection of internal standard gene generally requires copy number low
And stablize, general animal internal standard gene often selects on mitochondria, and such reference gene copy number is more, is not easy to quantitative.
The present invention selects the gene on the 29th chromosome as internal standard gene, and copy number is low, is easy to quantitative, compared on mitochondria
Gene, mutation rate is lower.
Fig. 1 is ring mediated isothermal amplification and colloidal gold nucleic acid test strip detection method schematic diagram.The present invention is anti-using LAMP
It answers, designs 4 primers for horse 6 specific regions of specificity internal standard gene target sequence to carry out isothermal duplication.LAMP's
Inner primer FIP and BIP mark biotin (Biotin) and fluorescein (FITC) respectively.It can produce by LAMP reaction a large amount of
Double stranded DNA target substance with biotin and fluorescein.Then LAMP reaction product is detected with colloidal gold nucleic acid test strip.Colloid
Golden nucleic acid test strip p-wire (TL) and nature controlling line (CL) are marked with FITC antibody and biotin secondary antibody respectively, in conjunction with being lined with colloid
Gold-biotin antibody marker.When LAMP reaction product is added drop-wise to test strips sample pad, due to capillarity, product meeting
Successively pass through bonding pad, detection line (TL), nature controlling line (CL), specific binding effect and colloidal gold due to " Ag-Ab "
Coagulation effect, when LAMP reaction product is positive, p-wire (TL) and nature controlling line (CL) have red stripes；Reaction product is yin
When property, then only nature controlling line (CL) has red stripes.
Colloidal gold nucleic acid test strip is the specific binding by antigen and antibody, therefore it is with high sensitivity.
The present invention is by passing through LAMP reaction bonded colloidal gold core for the mixing of the quality such as horseflesh minced meat and non-horseflesh minced meat progress gradient
Sour test strips are detected to probe into the sensitivity of this detection method.The results show that the detectable limit of detection kit of the present invention is
0.2% (w/w).
Based on present invention determine that for detecting the internal standard gene LOC106782588 of horse derived components in food, the present invention
The LAMP primer composition for detecting the gene is devised, can detect using LAMP reaction bonded colloidal gold nucleic acid test strip to be measured
Whether horse derived components are had in sample, this rapid reaction is time-consuming few, and specificity is good, and high sensitivity is easy to operate, does not need profession
Personnel's operation, is as a result easy to observe, and is very suitable for the use of base's food supervision and inspection.
Detailed description of the invention
Fig. 1 is the principle of the quick detection kit of ring mediated isothermal amplification and the detection of colloidal gold nucleic acid test strip；
Fig. 2 be horse specificity internal standard gene in 16 kinds of animals LAMP amplification, swimming lane 1 be horse LAMP product, 1:
Horse；2: pig；3: sheep；4: goat: 5: chicken；6: duck；7: goose: 8: ox；9: donkey；10: deer；11: yak；12: buffalo；13: ermine；
14: camel；15: fish；16: rat；M:Maker DL2000；
Fig. 3 is the positive judgement with negative findings in the detection of horse source LAMP product colloidal gold nucleic acid test strip；1: horse；2:
Chicken；3: ox；4: sheep: 5: pig；6: donkey；7: goose: 8: duck；9: goat；10: fish；11: yak；12: buffalo；13: rat；Sample 1
P-wire (TL) and nature controlling line (CL) there are red stripes then to prove positive sample, sample 2-13 only has nature controlling line (CL) to have
Red stripes are then negative sample；
Fig. 4 is that the detection sensitivity of LAMP- colloidal gold nucleic acid test strip is tested；1: 4 times of gradient of mixing, as original quality
25%；2: 20 times of gradient of mixing, as original quality 5%；3: 100 times of gradient of mixing, as original quality 1%；4: mixing
500 times of gradient, as original quality 0.2%；5: negative.
Specific embodiment
Following embodiment further illustrates the contents of the present invention, but should not be construed as limiting the invention.Without departing substantially from
In the case where spirit of that invention and essence, to modifications or substitutions made by the method for the present invention, step or condition, the present invention is belonged to
Range.
Unless otherwise specified, the conventional means that technological means used in embodiment is well known to those skilled in the art.
Pig (Sus scrofa), ox (Bos taurus), sheep (Ovis aries), common family chicken (Gallus gallus),
Pheasant (Phasianuscolchicus), turkey (Meleagris gallopavo), Gallus domesticlus brisson (Gallus domesticus
Brisson), duck (Anas platyrhynchos), goose (Goose calicivirus), dog (Canis lupus
Familiaris), rabbit (Oryctolagus cuniculus), yak (Bos mutus), yellow croaker (Pseudosciaena
Polyactis it) is bought for supermarket.Horse (Equus caballus), donkey (Equus asinus) are the purchase of Beijing market of farm produce.Always
Mouse (Mus musculus) is provided by China Agricultural University's food safety and Molecular Biology Lab.Buffalo (Bubalus
Bubalis), ermine (Martes zibellina), camel (Camelus ferus), deer (Cervus) are entered and left the border by Tianjin and are examined
Doctor Li Zongmeng of office provides.
The screening of the general internal standard gene LOC106782588 in 1 horse source of embodiment
By the gene information in search GenBank about horse, target gene group is downloaded from NCBI, and saves as
" .FASTA " format.Analyzed for the full-length genome information of horse, using 4.0 software of BLAST and DNAMAN Version into
Row homology analysis filters out LOC106782588 gene.By 20 kinds of meats (be common family chicken (Gallus gallus) respectively,
Pheasant (Phasianuscolchicus), turkey (Meleagris gallopavo), Gallus domesticlus brisson (Gallus domesticus
Brisson), pig (Sus scrofa), ox (Bos taurus), sheep (Ovis aries), duck (Anas platyrhynchos),
Goose (Goose calicivirus), dog (Canis lupus familiaris), rabbit (Oryctolagus cuniculus), yak
Ox (Bos mutus), yellow croaker (Pseudosciaena polyactis), horse (Equus caballus), donkey (Equus
Asinus), mouse (Mus musculus), buffalo (Bubalus bubalis), ermine (Martes zibellina), camel
(Camelus ferus), deer (Cervus)) LOC106782588 channel genes DNAMAN Version 4.0, carry out more sequences
Column specificity analysis, sequence alignment result save as " .seq " format.The high segment of selection specificity carries out BLAST analysis again,
Search sequence homology and specificity in database.It is integrated finally by sequence, determines final specific targets base
Because LOC106782588 gene can be used as internal standard gene.The nucleotide sequence of the LOC106782588 segment such as SEQ ID
Shown in NO.1.
The foundation of 2 horse derived components LAMP detection method of embodiment
Primer Photographing On-line software LAMP primer designing is mediated using Japanese Rong Yan Co., Ltd. ring
software primerexplorer V 5.0(http://primerexplorer.jp/elamp5.0.0/index.html)
LAMP primer, including 2 outer primers F3, B3 and 2 inner primers are designed for the LOC106782588 gene that embodiment 1 determines
FIP, BIP are shown in Table 1.5 ' end mark fluorescents of 5 ' end labels biotin (Biotin) of inner primer FIP, BIP are plain (FITC).
1 LAMP primer sequence of table
Horse sample, 25 μ L of reaction system, including 1x Thermopol buffer, 0.4mM are quickly detected using LAMP
DNTP, 3mMMgSO4, 1.0M glycine betaine, 1.6 μM of primers Fs IP, 1.6 μM of primer BIP, 0.2 μM of primers F 3,0.2 μM of primer B3,8U
BstDNA polymerase Large fragment.Response procedures are 65 DEG C of constant temperature 1h, 85 DEG C of 5min.After amplification, 2% Ago-Gel is utilized
Electrophoresis carries out product judgement, ladder-like band proof occurs and expands successfully, contains target gene.As a result as shown in Fig. 2, horse is special
Property internal standard gene in 16 kinds of animals LAMP amplification, swimming lane 1 be horse LAMP product, 1: horse；2: pig；3: sheep；4: mountain
Sheep: 5: chicken；6: duck；7: goose: 8: ox；9: donkey；10: deer；11: yak；12: buffalo；13: ermine；14: camel；15: fish；16: big
Mouse；M:MakerDL2000；Only there is bright band in horse sample, shows LOC106782588 gene and corresponding LAMP body
System can be used for the quick detection of horse type.
The foundation of 3 horse derived components LAMP product colloidal gold nucleic acid test strip detection method of embodiment
The preparation of colloidal gold labeled monoclonal antibody prepares colloidal gold using trisodium citrate improved method, and is purified with supercentrifugal process
Gold labeling antibody stores for future use the gold labeling antibody prepared at 4 DEG C.
FITC antibody is diluted to optium concentration with buffer respectively.P-wire (TL) between nature controlling line (CL) at a distance from be
4.5mm is sprayed on NC film respectively by 1.0 μ L/cm.It will be spare after 37 DEG C of NC film sprayed drying overnight.Test strips are cut into
3.8mm wide.
It is added dropwise in the sample pad of colloidal gold nucleic acid test strip after LAMP reaction product and buffer are sufficiently mixed, at this time
Mixed liquor passes through bonding pad and NC film under capillary power, and continues, 3min after i.e. observable inspection mobile to water absorption pad direction
Survey result.As shown in figure 3, the p-wire (TL) of sample 1 and nature controlling line (CL) have red stripes then to prove positive sample, sample
It is then negative sample that product 2-13, which only has nature controlling line (CL) to have red stripes,.1: horse；2: chicken；3: ox；4: sheep: 5: pig；6: donkey；7:
Goose: 8: duck；9: goat；10: fish；11: yak；12: buffalo；13: rat；
Colloidal gold nucleic acid test strip is the specific binding by antigen and antibody, therefore it is with high sensitivity.
Colloidal gold nucleic acid test strip is closed in advance with the BSA solution that concentration is 3%, before not influencing its normal positive colour developing
Put the appearance for avoiding false positive.By the way that horseflesh minced meat is carried out 5 times with non-horseflesh minced meat (ox, pig, mouse etc. mix minced meat)
The mixing of the quality such as gradient carries out LAMP reaction, then combines with colloidal gold nucleic acid test strip to probe into colloidal gold nucleic acid test paper
The sensitivity of detection method.As a result as shown in figure 4,1: 4 times of gradient of mixing, as the 25% of original quality；2: mixing gradient
20 times, as original quality 5%；3: 100 times of gradient of mixing, as original quality 1%；4: 500 times of gradient of mixing, it is as original
Quality 0.2%；5: negative.It is initial when mixing gradient is 500 times (mixing 500 times that minced meat quality is horseflesh minced meat quality)
Quality 0.2% when, detection T line is especially shallow, therefore the detection of detection kit of the present invention is limited to 0.2% (w/w).
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, without departing from the technical principles of the invention, several improvements and modifications can also be made, these improvements and modifications
Also it should be regarded as protection scope of the present invention.
Sequence table
<110>China Agricultural University
<120>horse derived components rapid detection method and kit in a kind of food
<130> MP1907471Z
<160> 5
<170> SIPOSequenceListing 1.0
<210> 1
<211> 300
<212> DNA
<213>horse (Equus caballus)
<400> 1
ccaaaccatt aatgtggatc tgaggagatg aggttctcca gggtgcagta aaagcccctc 60
atcacttaca gagctggttg cccaggatct gatggagcta tgtcttcaaa caaaggttgc 120
tagaaagttt gagctccaag ggaaggtaga gtcagaggac aaacctcaag gctgtgccac 180
tggcatgctc cttcaagact ctcatactga catcctcctt caagactatg ctcctcaaag 240
aatgtaccac caaaatgctc cttgctgcag aattcttggt ttctcacaag tcacagtcca 300
<210> 2
<211> 19
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 2
tggatctgag gagatgagg 19
<210> 3
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 3
ttgaggagca tagtcttgaa 20
<210> 4
<211> 39
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 4
atagctccat cagatcctgg gccagggtgc agtaaaagc 39
<210> 5
<211> 42
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 5
caagggaagg tagagtcaga ggggaggatg tcagtatgag ag 42
Claims (10)
1. it is a kind of for detecting the gene of horse derived components in food, it is internal standard gene, there is sequence shown in SEQ ID NO.1
Column.
2. application of the gene described in claim 1 in detection horse derived components.
3. application of the gene described in claim 1 in food horse Components identification.
4. the specific LAMP primer composition for detecting gene described in claim 1, including sequence such as SEQ ID NO.2-5 institute
Following 4 primers shown:
F3:5 '-TGGATCTGAGGAGATGAGG-3 '；
B3:5 '-TTGAGGAGCATAGTCTTGAA-3 '；
FIP:5 '-ATAGCTCCATCAGATCCTGGGCCAGGGTGCAGTAAAAGC-3 '；
BIP:5 '-CAAGGGAAGGTAGAGTCAGAGGGGAGGATGTCAGTATGAGAG-3 '.
5. application of the specificity LAMP primer composition as claimed in claim 4 in the identification of horse derived components.
6. specificity LAMP primer composition as claimed in claim 4 is in preparation horse derived components detection kit or detection reagent
Using.
7. the rapid detection method of horse derived components in a kind of detection food, which comprises the following steps:
(1) sample to be tested extracts DNA；
(2) to extract DNA as template, LAMP detection is carried out using specificity LAMP primer composition described in claim 4；
(3) result judges: carrying out result judgement using colloidal gold nucleic acid test strip, detecting T line and Quality Control C line has red bar
Band contains target gene；Quality Control C line has red stripes, and detects T line without band, does not contain target gene.
8. the method for claim 7, which is characterized in that step (2) the LAMP detection, 25 μ L LAMP detect body
The concrete configuration of system are as follows: 1x Thermopol buffer, 0.4mM dNTP, 3mM MgSO4, 1.0M glycine betaine, 1.6 μM of primers
FIP, 1.6 μM of primer BIP, 0.2 μM of primers F, 3,0.2 μM of primer B3,8U Bst archaeal dna polymerase large fragment.
9. method as claimed in claim 7 or 8, which is characterized in that LAMP detects reaction condition are as follows: 60-65 DEG C of constant temperature
Then 20min, 85 DEG C of 5min terminate reaction.
10. method as claimed in claim 7 or 8, which is characterized in that colloidal gold nucleic acid test strip described in step (3) includes
Colloidal gold nucleic acid test strip p-wire and nature controlling line, p-wire are marked with FITC antibody, and nature controlling line is marked with biotin secondary antibody, knot
Conjunction is lined with colloidal gold-biotin antibody marker.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910290268.6A CN109852705B (en) | 2019-04-11 | 2019-04-11 | Method and kit for rapidly detecting horse-derived components in food |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910290268.6A CN109852705B (en) | 2019-04-11 | 2019-04-11 | Method and kit for rapidly detecting horse-derived components in food |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109852705A true CN109852705A (en) | 2019-06-07 |
| CN109852705B CN109852705B (en) | 2021-01-01 |
Family
ID=66903834
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910290268.6A Active CN109852705B (en) | 2019-04-11 | 2019-04-11 | Method and kit for rapidly detecting horse-derived components in food |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109852705B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112501320A (en) * | 2021-02-08 | 2021-03-16 | 中国农业大学 | Snake origin component rapid detection kit and application thereof |
| CN112680531A (en) * | 2021-01-22 | 2021-04-20 | 华中农业大学 | Primer pair, kit and method for quickly detecting horse-derived components in horse skins and mule skins |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102311998A (en) * | 2011-08-31 | 2012-01-11 | 深圳出入境检验检疫局动植物检验检疫技术中心 | Horse or horse source component real time fluorescent PCR detection method and primers and probe for detection |
| CN103630619A (en) * | 2013-10-28 | 2014-03-12 | 山东东阿阿胶股份有限公司 | Detection substance for tortoise-shell glue and products thereof and MS (Mass Spectrometry) detection method thereof |
| CN104250667A (en) * | 2014-10-09 | 2014-12-31 | 北京农学院 | Detecting method for horse source elements in meat and meat products and detecting method for donkey source element in meat and meat products |
| CN107290540A (en) * | 2017-06-22 | 2017-10-24 | 中国农业大学 | Escherichia coli O 157:H7 and the common Test paper of salmonella typhimurium and preparation method and application |
-
2019
- 2019-04-11 CN CN201910290268.6A patent/CN109852705B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102311998A (en) * | 2011-08-31 | 2012-01-11 | 深圳出入境检验检疫局动植物检验检疫技术中心 | Horse or horse source component real time fluorescent PCR detection method and primers and probe for detection |
| CN103630619A (en) * | 2013-10-28 | 2014-03-12 | 山东东阿阿胶股份有限公司 | Detection substance for tortoise-shell glue and products thereof and MS (Mass Spectrometry) detection method thereof |
| CN104250667A (en) * | 2014-10-09 | 2014-12-31 | 北京农学院 | Detecting method for horse source elements in meat and meat products and detecting method for donkey source element in meat and meat products |
| CN107290540A (en) * | 2017-06-22 | 2017-10-24 | 中国农业大学 | Escherichia coli O 157:H7 and the common Test paper of salmonella typhimurium and preparation method and application |
Non-Patent Citations (3)
| Title |
|---|
| GENBANK: "LOC106782588", 《GENBANK》 * |
| MARIA MAGIATI ET,AL.: "Lateral flow test for meat authentication with visual detection", 《FOOD CHEMISTRY》 * |
| 罗云波等: "核酸侧流层析传感器的构建及其在食品安全快速检测中的应用", 《中国食品学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112680531A (en) * | 2021-01-22 | 2021-04-20 | 华中农业大学 | Primer pair, kit and method for quickly detecting horse-derived components in horse skins and mule skins |
| CN112501320A (en) * | 2021-02-08 | 2021-03-16 | 中国农业大学 | Snake origin component rapid detection kit and application thereof |
| CN112501320B (en) * | 2021-02-08 | 2021-04-27 | 中国农业大学 | Snake origin component rapid detection kit and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109852705B (en) | 2021-01-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110055340A (en) | Bovine material rapid detection method and kit in a kind of food | |
| CN105177150B (en) | A kind of the multiple PCR primer system and detection method of quick detection pig sheep ox animal derived materials | |
| CN109852705A (en) | Horse derived components rapid detection method and kit in a kind of food | |
| CN109868321B (en) | Multiple fluorescence PCR method and kit for identifying animal-derived components | |
| CN114752690A (en) | Method for rapidly identifying duck-origin components in meat products based on MIRA technology | |
| CN110055339B (en) | Quick detection kit for duck source components in food and application thereof | |
| CN109825613A (en) | Duck derived components rapid detection method and kit in a kind of food | |
| CN109852707A (en) | Chicken derived components rapid detection method and kit in a kind of food | |
| CN109825610B (en) | Kit for rapidly detecting horse-derived components in food and application thereof | |
| CN109825612B (en) | Kit for rapidly detecting bovine-derived components in food and application thereof | |
| CN109825611A (en) | Goose derived components rapid detection method and kit in a kind of food | |
| CN110195112A (en) | Pig derived components rapid detection method and kit in a kind of food | |
| CN109811066A (en) | Sheep derived components rapid detection method and kit in a kind of food | |
| CN109897902A (en) | Donkey derived components quickly detect method for testing and kit in a kind of food | |
| CN109811067A (en) | Chicken derived components quick detection kit and its application in a kind of food | |
| CN109811069A (en) | Donkey derived components quick detection kit and its application in a kind of food | |
| CN109825609A (en) | The kit of bovine material and its application in a kind of Rapid identification food | |
| CN109852706A (en) | The kit of duck derived components and its application in a kind of Rapid identification food | |
| CN109811065B (en) | Rapid detection kit for goose-derived components in food and application thereof | |
| CN109825606B (en) | Kit for rapidly detecting pig-derived components in food and application thereof | |
| CN109852709B (en) | Kit for rapidly detecting goat-derived components in food and application thereof | |
| CN110195111B (en) | Kit for rapidly detecting sheep-derived components in food and application thereof | |
| CN109825607B (en) | Kit for rapidly identifying donkey-derived components in food and application thereof | |
| CN108251534A (en) | A kind of quick detection meat source food multiple PCR detection kit | |
| CN109852708B (en) | Kit for rapidly identifying goose-derived components in food and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |