CN109852372A - A kind of CsPbBr of phospholipid micelles encapsulation3Nanocrystalline composite material and its application - Google Patents
A kind of CsPbBr of phospholipid micelles encapsulation3Nanocrystalline composite material and its application Download PDFInfo
- Publication number
- CN109852372A CN109852372A CN201811504153.4A CN201811504153A CN109852372A CN 109852372 A CN109852372 A CN 109852372A CN 201811504153 A CN201811504153 A CN 201811504153A CN 109852372 A CN109852372 A CN 109852372A
- Authority
- CN
- China
- Prior art keywords
- cspbbr
- nanocrystalline
- solution
- composite material
- phospholipid micelles
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The imaging of Hella cell is realized the invention discloses a kind of preparation method of water-soluble calcium titanium ore and as image probe.The present invention, which mainly passes through phosphatide and the hydrophobe interaction of oleyl amine, oleic acid, realizes phospholipid micelles to CsPbBr3Nanocrystalline encapsulation.The CsPbBr of resulting phospholipid micelles encapsulation3It is nanocrystalline, it can be dissolved in water, emit efficient green fluorescence, and present the stability of superelevation in water.In addition, this material has preferable biocompatibility, the imaging of high quality Hella cell is successfully realized.
Description
Technical field
The invention belongs to stabilization, the exploitation of bio-compatible fluorescence probe and application field, in particular to a kind of phospholipid micelles envelope
The CsPbBr of dress3Nanocrystalline composite material and its application.
Background technique
The past in recent years, CsPbBr3It is nanocrystalline to be used as star's material, attract great concern and research,
It has been widely used on light emitting diode, devices photovoltaic, sensor and imaging.Because of CsPbBr3The preparation of perovskite
It is simple and cheap.It is prior, it is obtained nanocrystalline usually to show optical property outstanding and charge carrier mobility.
For example, its high luminous efficiency and narrow luminous halfwidth, have greatly pushed CsPbBr3It is nanocrystalline in light emitting diode, it is aobvious
Show the application on device and imaging.However, due to big specific surface area and high activity, CsPbBr3It is nanocrystalline often suffer from difference
Stability, this to a certain degree on limit their practical application, the especially imaging of cell.In general, when there are water, by
In aquation, CsPbBr3Nanocrystalline lattice will be destroyed, and lead to the disappearance of optical property or the leakage of lead ion, this is greatly
Hinder the CsPbBr with high-efficiency fluorescence3The nanocrystalline application on cell imaging.Therefore, CsPbBr is utilized3It is nanocrystalline excellent
Optical property realize high quality cell imaging be all the time one greatly challenge.
In order to realize the application of cell imaging and biomarker, CsPbBr3It is nanocrystalline to couple some suitable protections
Material goes to overcome the attack of water.In the recent period, many effort are done, and for enhancing the resistivity of water, go to delay or prevent light
The degeneration for learning property, such as the encapsulation of stable inorganic material or waterrepellent polymeric matrix.For example, comparing silester based on methyl silicate
The partners of higher hydrolysis rate, Lee and Ta have synthesized SiO in analytically pure toluene2The CH of encapsulation3NH3PbBr3Quantum
Point.Although excellent optical stabilization performance is obtained, due to SiO under the LED light of 470nm2Often there is mesoporous or
Micropore can not fully hinder the entrance of hydrone, lead to the degeneration of optical property and the leakage of lead ion.The conjunction of woods and he
Authors have synthesized perovskite-polyphenyl of high fluorescence by swelling and shrinkage character of the polystyrene in toluene and hexamethylene
Ethylene composite ball, obtained composite ball present strong water resistant ability.But the ball of these green fluorescences is mainly distributed
At the edge of MCF-7 cell, this may be caused by oversized or low cellular uptake amount.As known, in addition to material
The halfwidth of material is narrow, and efficient outer, the small size that shines, good biocompatibility and high cellular uptake ability are also usually connect
By, improve cell imaging quality on play important role.Therefore, in order to pass through high fluorescence CsPbBr3It is nanocrystalline, it is real
Existing high quality imaging applications further promote the stability in water, reduce size, and the intake of enhancing cell is all urgent need to resolve
The problem of.
In view of the deficiencies of the prior art, it is an object of the present invention to provide a kind of CsPbBr of phospholipid micelles encapsulation3It is nanocrystalline
The compound of composite material.And it is applied to the imaging of Hella cell, the CsPbBr of phospholipid micelles encapsulation3Nanocomposite material
Expect the water solubility that has had, optical stability in excellent water, biocompatibility outstanding, the Hella for realizing high quality is thin
The imaging of born of the same parents.
Summary of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide a kind of CsPbBr of phospholipid micelles encapsulation3Nanometer
Crystal composite material and its application.
The purpose of the present invention is what is be achieved through the following technical solutions: a kind of CsPbBr of phospholipid micelles encapsulation3It is nanocrystalline
Composite material is prepared by the following method to obtain:
(1)0.4mmol PbBr2The DMF of 10mL, then, 1mL oleic acid are dissolved in by ultrasound with 0.4mmol CsBr
It is added in solution in the case of stirring with 0.5mL oleyl amine as stabilizer, the solution of acquisition is further as presoma
It uses.
(2) precursor solution of 1mL is taken to be injected into the toluene of 15mL in the case of stirring.After stirring 30s, solution quilt
8000 turns of centrifugation 5min, take supernatant as CsPbBr3Nanocrystalline mother liquor is further modified afterwards.Above-mentioned all operations are all in room
Temperature is lower to be carried out and (not to need the DMF inside removal here).
(3) CsPbBr of 4mL is taken3The toluene solution of the DSPE-PEG2000 (10mg/mL) of nanocrystalline mother liquor and 2mL mix
Together, at room temperature, 30min is stood.
(4) by the toluene in (3) solution, under vacuum, revolving is removed.
(5) it is added to 15mL water is adherent in (4) in residual solution, slowly rocks and realize nanocrystalline aquation.
(6) it by the super filter tube of (5) merging 20100KDa deadline size, washes three times for 4500 turns and obtains corresponding sample
A kind of CsPbBr of phospholipid micelles encapsulation3The application of nanocrystalline composite material, the application are by the material application
In the imaging of Hella cell.
The beneficial effects of the present invention are: the present invention passes through solvent pairs exchange process, in phosphatide-polyethylene glycol and oleyl amine, oleic acid
Hydrophobe effect induction self assembly driving under, be successfully prepared for the CsPbBr of water-soluble phospholipid micelles package3It receives
The brilliant compound of rice.The compound of this preparation not only presents high-efficiency fluorescence efficiency, but also illustrates superpower water resistant ability.More
Important, the CsPbBr of phospholipid micelles package3It is nanocrystalline that also there is very excellent biocompatibility.Based on these excellent property
Matter, the CsPbBr of phospholipid micelles package3It is nanocrystalline to be used as fluorescence probe, realize the high quality imaging of Hella cell.
Detailed description of the invention
Fig. 1 is the CsPbBr of phospholipid micelles encapsulation prepared by the present invention3The transmission electron microscope of nanocrystalline composite material
Picture (TEM).
Fig. 2 is the CsPbBr that the present invention prepares phospholipid micelles encapsulation3What nanocrystalline composite material changed over time in water
Photoluminescence spectra.
Fig. 3 is the CsPbBr that the present invention prepares phospholipid micelles encapsulation3Nanocrystalline composite material and Hella cell co-culture
Co-focusing imaging figure after for 24 hours.
Specific embodiment
Below with reference to embodiment the invention will be further described technical solution of the invention, these embodiments cannot
It is not understood as the limitation to technical solution.
Embodiment 1: the present embodiment prepares the CsPbBr of phospholipid micelles encapsulation3Nanocrystalline composite material specifically includes following
Step:
(1)0.4mmol PbBr2The DMF of 10mL, then, 1mL oleic acid are dissolved in by ultrasound with 0.4mmol CsBr
It is added in solution in the case of stirring with 0.5mL oleyl amine as stabilizer, the solution of acquisition is further as presoma
It uses.
(2) precursor solution of 1mL is taken to be injected into the toluene of 15mL in the case of stirring.After stirring 30s, solution quilt
8000 turns of centrifugation 5min, take supernatant as CsPbBr3Nanocrystalline mother liquor is further modified afterwards.Above-mentioned all operations are all in room
Temperature is lower to be carried out and (not to need the DMF inside removal here).
(3) CsPbBr of 4mL is taken3The toluene solution of the DSPE-PEG2000 (10mg/mL) of nanocrystalline mother liquor and 2mL mix
Together, at room temperature, 30min is stood.
(4) by the toluene in (3) solution, under vacuum, revolving is removed.
(5) it is added to 15mL water is adherent in (4) in residual solution, slowly rocks and realize nanocrystalline aquation.
(6) it by the super filter tube of (5) merging 20100KDa deadline size, washes three times for 4500 turns and obtains corresponding sample.
Fig. 1 is the transmission electron microscopy of the CsPbBr3 nanocrystalline composite material of standby phospholipid micelles encapsulation prepared by the present invention
Mirror figure (TEM), it can be seen that multiple CsPbBr3It is nanocrystalline to gather together, illustrate that phospholipid micelles encapsulate nanometer well
It is brilliant.
Embodiment 2 has studied the CsPbBr of the phospholipid micelles encapsulation of the preparation of embodiment 13Nanocomposite object is in water
Stability.Specifically: the CsPbBr for encapsulating the phospholipid micelles of 0.5mg3Nanocomposite object is added to the deionization of 3mL volume
In water, as time change tests its photoluminescence spectrum under 365nm excitation.As shown in Fig. 2, within 84h, fluorescence intensity and
Halfwidth there is no apparent variation, illustrate preferable stability, show that its material has excellent water resistant ability.
Embodiment 3: the CsPbBr of phospholipid micelles encapsulation prepared by embodiment 13Nanocomposite object and Hella cell are total
Culture, is imaged, specific embodiment is as follows under confocal microscope:
The CsPbBr that the phospholipid micelles of 1.5ug/mL are encapsulated3Nanocomposite object and Hella cell are in cell incubator
Middle co-cultivation for 24 hours, then takes out and is placed under Laser Scanning Confocal Microscope, in the receiving channel of 405nm laser channeling and 480-550nm
Under, be imaged while cell light field and dark field.As shown in figure 3, green fluorescence point all concentrates on the interior of Hella cell
Portion shows the CsPbBr of phospholipid micelles encapsulation3It is nanocrystalline can be by Hella cellular uptake, without inactivating.It shows high-quality
The imaging results of the Hella cell of amount.
The CsPbBr of the phospholipid micelles encapsulation of the method for the present invention preparation3The halogen perovskite of nanocrystalline very good solution is in water
In unstable problem, while also enhancing the intake ability of its cell compatibility and cell.Relative to other by halogen calcium titanium
The mining report in cell imaging illustrates the cell imaging figure of high quality.
Claims (3)
1. a kind of CsPbBr of phospholipid micelles encapsulation3Nanocrystalline composite material, which is characterized in that the material can pass through following step
Suddenly it is prepared:
(1) CsPbBr of 4mL is taken3The toluene solution of the DSPE-PEG2000 (10mg/mL) of nanocrystalline mother liquor and 2mL are blended in one
It rises, at room temperature, stands 30min.
(2) under vacuum, revolving removes toluene.
(3) adherent be added to of 15mL water in residual solution, is rocked into the nanocrystalline aquation of realization in (2).
(4) it is transferred in the super filter tube of 20100KDa deadline size, washes three times for 4500 turns and obtain corresponding sample.
2. composite material according to claim 1, which is characterized in that the CsPbBr3Nanocrystalline mother liquor is by the following method
It is prepared:
(1.1)0.4mmol PbBr2It is dissolved in the DMF of 10mL with 0.4mmol CsBr by ultrasound, then by 1mL oleic acid
It is added in DMF solution in the case of stirring with 0.5mL oleyl amine as stabilizer, the solution of acquisition is as presoma into one
Step uses.
(1.2) precursor solution of 1mL is taken to be injected into the toluene of 15mL in the case of stirring.After stirring 30s, solution quilt
8000 turns of centrifugation 5min, take supernatant as CsPbBr3Nanocrystalline mother liquor.
3. a kind of CsPbBr of phospholipid micelles encapsulation described in claim 13Nanocrystalline composite material is in Hella cell imaging
Application.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811504153.4A CN109852372B (en) | 2018-12-10 | 2018-12-10 | Phospholipid micelle encapsulated CsPbBr3Nanocrystalline composite material and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811504153.4A CN109852372B (en) | 2018-12-10 | 2018-12-10 | Phospholipid micelle encapsulated CsPbBr3Nanocrystalline composite material and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109852372A true CN109852372A (en) | 2019-06-07 |
| CN109852372B CN109852372B (en) | 2021-10-29 |
Family
ID=66890854
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811504153.4A Active CN109852372B (en) | 2018-12-10 | 2018-12-10 | Phospholipid micelle encapsulated CsPbBr3Nanocrystalline composite material and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109852372B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111072717A (en) * | 2019-12-06 | 2020-04-28 | 吉林大学 | Preparation method of organic-inorganic hybrid perovskite material with good stability in water |
| CN114295591A (en) * | 2021-12-01 | 2022-04-08 | 无锡市第二人民医院 | Double-signal nano probe and application thereof in detection of prostate specific antigen |
| CN114958367A (en) * | 2022-06-09 | 2022-08-30 | 南京工业大学 | Preparation of water-soluble lead-calcium-titanium halide ore nanocrystal and biological imaging application |
| CN115072767A (en) * | 2021-03-10 | 2022-09-20 | 南京大学 | Based on bionical CsPbBr 3 Method for detecting pore-forming toxin by NCs electrochemical and fluorescence dual-signal sensor |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107880875A (en) * | 2017-12-11 | 2018-04-06 | 东南大学 | Cell imaging probe based on full-inorganic perovskite quantum dot and preparation method thereof |
| CN108034419A (en) * | 2017-12-11 | 2018-05-15 | 东南大学 | A kind of water solubility full-inorganic perovskite quantum dot and preparation method thereof |
-
2018
- 2018-12-10 CN CN201811504153.4A patent/CN109852372B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107880875A (en) * | 2017-12-11 | 2018-04-06 | 东南大学 | Cell imaging probe based on full-inorganic perovskite quantum dot and preparation method thereof |
| CN108034419A (en) * | 2017-12-11 | 2018-05-15 | 东南大学 | A kind of water solubility full-inorganic perovskite quantum dot and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| MICHAEL J. MURCIA等: "Fluorescence correlation spectroscopy of CdSe/ZnS quantum dot optical bioimaging probes with ultra-thin biocompatible coatings", 《OPTICS COMMUNICATIONS 》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111072717A (en) * | 2019-12-06 | 2020-04-28 | 吉林大学 | Preparation method of organic-inorganic hybrid perovskite material with good stability in water |
| CN111072717B (en) * | 2019-12-06 | 2022-11-08 | 吉林大学 | Preparation method of organic-inorganic hybrid perovskite material with good stability in water |
| CN115072767A (en) * | 2021-03-10 | 2022-09-20 | 南京大学 | Based on bionical CsPbBr 3 Method for detecting pore-forming toxin by NCs electrochemical and fluorescence dual-signal sensor |
| CN115072767B (en) * | 2021-03-10 | 2024-03-29 | 南京大学 | Based on bionical CsPbBr 3 Method for detecting pore-forming toxin by using electrochemical and fluorescent double-signal sensor of NCs |
| CN114295591A (en) * | 2021-12-01 | 2022-04-08 | 无锡市第二人民医院 | Double-signal nano probe and application thereof in detection of prostate specific antigen |
| CN114958367A (en) * | 2022-06-09 | 2022-08-30 | 南京工业大学 | Preparation of water-soluble lead-calcium-titanium halide ore nanocrystal and biological imaging application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109852372B (en) | 2021-10-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109852372A (en) | A kind of CsPbBr of phospholipid micelles encapsulation3Nanocrystalline composite material and its application | |
| Liu et al. | Three models to encapsulate multicomponent dyes into nanocrystal pores: A new strategy for generating high-quality white light | |
| Tian et al. | Synthesis and luminescent properties of spindle-like CaWO4: Sm3+ phosphors | |
| Han et al. | Defect-sensitive crystals based on diaminomaleonitrile-functionalized Schiff base with aggregation-enhanced emission | |
| CN108034418B (en) | All-inorganic lead-halogen perovskite nano composite luminescent material, and preparation method and application thereof | |
| CN105062485B (en) | Nano/micron crystal preparation method is changed on gadolinium ion doping fluorination lutetium sodium | |
| CN110117024A (en) | A kind of unleaded caesium copper iodine perovskite blue light of zero dimension is nanocrystalline and preparation method thereof | |
| CA2939467C (en) | Luminescent hybrid nanomaterials with aggregation induced emission | |
| Cong et al. | Synthesis and optical properties of Zn2+ doped NaYF4: Yb3+, Er3+ upconversion nanoparticles | |
| Xu et al. | Preparation and properties of dual-mode luminescent NaYF 4: Yb, Tm@ SiO 2/carbon dot nanocomposites | |
| An et al. | Non-blinking, highly luminescent, pH-and heavy-metal-ion-stable organic nanodots for bio-imaging | |
| Das et al. | Selective binding of genomic Escherichia coli DNA with ZnO leads to white light emission: a new aspect of nano–bio interaction and interface | |
| CN109233807B (en) | Rare earth composite luminescent hydrogel material and preparation method and application thereof | |
| Venkatachalam et al. | Synthesis and toxicity assay of ceramic nanophosphors for bioimaging with near-infrared excitation | |
| CN110257061A (en) | Method for preparing yellow fluorescence carbon dots using 2,3- diamino-pyridine and products thereof and application | |
| Wu et al. | Smartphone‐based rapid and visual pathological diagnosis of glioma using perovskite probes | |
| CN108998029A (en) | A kind of water solubility NaYF4:Yb3+,Er3+@NaGdF4The preparation method of crystal grain | |
| Bagnall et al. | Mesoscopic FRET Antenna Materials by Self‐Assembling Iridium (III) Complexes and BODIPY Dyes | |
| CN109294577B (en) | NaYF4Eu @ CDs composite material and preparation method and application thereof | |
| Wu et al. | One-pot synthesis of hollow structured upconversion luminescent β-NaYF4: Yb0. 2Er0. 02 nanoparticles | |
| CN108714223B (en) | Contrast agent with magnetic resonance and fluorescence dual imaging characteristics and preparation method thereof | |
| Topolska et al. | The effect of gluconic acid secretion by phosphate-solubilizing Pseudomonas putida bacteria on dissolution of pyromorphite Pb_ {5}(PO_ {4}) _ {3} Cl and Pb remobilization | |
| CN115433567A (en) | Organic room temperature phosphorescent eutectic material and preparation method thereof | |
| CN106244145B (en) | A kind of method of the CdSe quantum dot of synthesis in water rare earth Eu doping | |
| CN109504364A (en) | Preparation method of one-dimensional organic core-shell structure micron crystal material |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |