CN109847097A - Intrinsic bioremediation film, preparation method and application - Google Patents
Intrinsic bioremediation film, preparation method and application Download PDFInfo
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Abstract
The invention discloses a kind of intrinsic bioremediation film, preparation method and applications.The intrinsic bioremediation film raw material is made of water soluble chitosan, alginate, Isin glue collagen active peptide and auxiliary material.Intrinsic bioremediation film provided by the invention uses natural bioactive material to be made completely, switch to hydrogel docile at ulcer after absorbing liquid, it not only can provide physical barriers and reduce pain caused by friction, ulcer wound surface reparation can also be effectively facilitated, reduce stimulation of the inflammatory reaction to the surface of a wound, various aspects mitigate patient suffering.In addition, canker sore provided by the invention does not add any medicine components or chemical reagent with intrinsic bioremediation film, no drug abuse risk is highly-safe.
Description
Technical field
The present invention relates to the field of medical instrument technology, in particular to a kind of intrinsic bioremediation film, preparation method
And application.
Background technique
Canker sore is a kind of common ulcerative damages illness for betiding mucous membrane of mouth.Local trauma, stress,
Food, drug, malnutrition, hormonal readiness change and many factors such as vitamin or microelement deficiencies can lead to oral cavity and burst
Ulcer.Sharp ache when canker sore is broken out, local cusalgia is obvious, and serious person also will affect diet, speak, and causes to daily life
It is greatly inconvenient, it can also cause chronic pharyngitis, have a headache and fever, the constitutional symptoms such as enlargement of lymph nodes.
Existing dental ulcer treatment mainly includes antibiotics (such as antibiotic mouthwash, antibiotic mostly based on drug
Medicine film), steroids (such as oral dexamethasone ulcer pad pasting), Chinese medicine class (such as Erhuang mouth ulcer is clear) and biomaterial class (such as seaweed
Acids canker sore patch) etc., wherein antibiotics and steroids clinical use are strictly controlled and that drug has been used for multiple times is resistance to
By risk, natural biologic material class canker sore repair materials have both moist more invasive and repair induction type, and use is safer
Effectively, therefore exploitation canker sore intrinsic bioremediation film has good potential applicability in clinical practice, but part canker sore is used
Repair membrane is using cellulose family or seaweed acrylic materials as substrate, most but since the antibacterial anti-inflammatory of substrate itself is not good enough
Repair membrane class product still contains medicine components, belongs to medical instruments combination product.
Summary of the invention
The present invention is intended to provide a kind of intrinsic bioremediation film, preparation method and application, are used for canker sore to improve
Drug safety.
To achieve the goals above, according to an aspect of the invention, there is provided a kind of intrinsic bioremediation film.This is natural
Biological prosthetic film raw material is made of water soluble chitosan, alginate, Isin glue collagen active peptide and auxiliary material.
Further, the mass ratio of water soluble chitosan and alginate is 1:1~10:1, Isin glue collagen active peptide matter
Amount is the 0.01%~10.0% of water soluble chitosan quality.
Further, molecular weight >=150KDa of water soluble chitosan, deacetylation 60~98%, degree of substitution >=0.8；Sea
Molecular weight >=100KDa of alginates, M/G >=1.0；Molecular weight≤3000Da of Isin glue collagen active peptide.
Further, water soluble chitosan is selected from by carboxymethyl chitosan/chitin, hydroxypropyl chitosan/chitin, hydroxyl
Ethyl chitosan/chitin, hydroxyl butyl chitosan/chitin, chitosan quaternary ammonium salt, chitosan lactate, chitosan glutamate
One of group of salt and sulfated chitosan composition is a variety of.
Further, alginate be selected from one of group for being made of sodium alginate, potassium alginate and ammonium alginate or
It is a variety of.
Further, fish-skin, fish scale or fish-bone of the Isin glue collagen active peptide from marine fishes or freshwater fish.
Further, the molecular weight of water soluble chitosan is more than or equal to 200KDa and is less than or equal to 500KDa, deacetylation 75
~95%, degree of substitution >=0.85；The molecular weight of alginate is more than or equal to 100KDa and is less than or equal to 500KDa, M/G >=1.2；Fish
Molecular weight≤3000Da of collagen active peptide.
Further, the mass ratio of water soluble chitosan and alginate is 3:2~5:1.
Further, intrinsic bioremediation film is applied to canker sore.
According to another aspect of the present invention, a kind of preparation method of any of the above-described kind of intrinsic bioremediation film is provided.It should
Preparation method is the following steps are included: a kind of canker sore intrinsic bioremediation film, and preparation includes the following steps: S1, by water
The physiological saline solution of soluble chitosan and Isin glue collagen active peptide containing 0.5% calcium chloride, is configured to solution A；S2, will be extra large
Alginates are configured to solution B with physiological saline；And S3, solution A and solution B are blended in lyophilised state pushing film-like and obtained
Intrinsic bioremediation film.
Further, the concentration of water soluble chitosan is 1.5~4wt% in solution A, and Isin glue collagen active peptide quality is
The 0.01%~10.0% of water soluble chitosan quality.
Further, after solution A and solution B mix, the mass ratio of water soluble chitosan and alginate is 1:1~10:
1。
Further, the preparation of solution A and solution B aseptically carries out；It preferably, further include to day after S3
So biological prosthetic film carries out the step of irradiation sterilization.
Intrinsic bioremediation film provided by the invention uses natural bioactive material to be made completely, switchs to after absorbing liquid
Hydrogel docile not only can provide physical barriers and reduce pain caused by friction, can also effectively facilitate ulcer wound surface at ulcer
It repairs, reduce stimulation of the inflammatory reaction to the surface of a wound, various aspects mitigate patient suffering.In addition, canker sore provided by the invention is used
Intrinsic bioremediation film does not add any medicine components or chemical reagent, and no drug abuse risk is highly-safe.
Specific embodiment
It should be noted that in the absence of conflict, the features in the embodiments and the embodiments of the present application can phase
Mutually combination.Below in conjunction with embodiment, the present invention will be described in detail.
A kind of typical embodiment according to the present invention provides a kind of intrinsic bioremediation film.The intrinsic bioremediation film
Raw material is made of water soluble chitosan, alginate, Isin glue collagen active peptide and auxiliary material.
Wherein, chitosan substance have the function of it is antibacterial, hemostasis, promote more, analgesic, epithelial cell can be promoted, at fiber
The division of the various kinds of cell such as cell and extracellular matrix growth, hence it is evident that accelerate the healing of the surface of a wound and reduce scar to be formed, can be used as function
Property dressing.Seaweed acids product can keep the Moist healing environment of wound, at the same can also by the bacterium of wound surface, inflammatory because
Son, tissue or cell debris etc. are locked in inside gel, are reached clinical debridement effect, be can be used as the product of wound care.Collagen egg
White active peptide has the effects that removing free radical, induced cell proliferation, wound healing, can also behave as antibacterial peptide activity.
Intrinsic bioremediation film provided by the invention uses natural bioactive material to be made completely, switchs to after absorbing liquid
Hydrogel docile not only can provide physical barriers and reduce pain caused by friction, can also effectively facilitate ulcer wound surface at ulcer
It repairs, reduce stimulation of the inflammatory reaction to the surface of a wound, various aspects mitigate patient suffering.In addition, canker sore provided by the invention is used
Intrinsic bioremediation film does not add any medicine components or chemical reagent, and no drug abuse risk is highly-safe.
In addition, the commercially available purchase of three kinds of Main Components, cost and risk needed for the present invention are controllable；It is provided by the invention
Intrinsic bioremediation film can be changed into hydrogel state and attach in affected part after being used for ulcer wound surface, form moist barrier, reduce with
The friction of its hetero-organization or substance, and can promote ulcer wound surface healing, inhibit inflammatory reaction and reduce infection and other effects；The present invention
Drug containing or other substances, no drug abuse risk be not highly-safe for the intrinsic bioremediation film of offer.
Preferably, the mass ratio of water soluble chitosan and alginate is 1:1~10:1, more preferably 3:2~5:1；Fish
Collagen active peptide quality is the 0.01%~10.0% of water soluble chitosan quality.
Preferably, molecular weight >=150KDa of water soluble chitosan, deacetylation 60~98%, degree of substitution >=0.8；Seaweed
Molecular weight >=100KDa of hydrochlorate, M/G >=1.0；Molecular weight≤3000Da of Isin glue collagen active peptide.Above-mentioned performance is limited to refer to
Mark, can largely optimize the performance indicator of intrinsic bioremediation film.It is furthermore preferred that the molecular weight of water soluble chitosan
It is less than or equal to 500KDa, deacetylation 75~95%, degree of substitution >=0.85 more than or equal to 200KDa；The molecular weight of alginate is big
It is less than or equal to 500KDa, M/G >=1.2 in being equal to 150KDa；Molecular weight≤3000Da of Isin glue collagen active peptide, thus into one
The performance indicator of step optimization intrinsic bioremediation film.
A kind of typical embodiment according to the present invention, water soluble chitosan are selected from by carboxymethyl chitosan/chitin, hydroxyl
Propyl chitosan/chitin, hydroxyethyl chitosan/chitin, hydroxyl butyl chitosan/chitin, chitosan quaternary ammonium salt, chitosan
One of group of lactate, glutamate and sulfated chitosan composition is a variety of；Alginate is selected from by seaweed
One of group of sour sodium, potassium alginate and ammonium alginate composition is a variety of；Isin glue collagen active peptide derives from marine fishes
Or fish-skin, fish scale or the fish-bone of freshwater fish.
A kind of typical embodiment according to the present invention, intrinsic bioremediation film are applied to canker sore, can also answer certainly
Reparation for other similar wound.
A kind of typical embodiment according to the present invention provides a kind of preparation method of above-mentioned intrinsic bioremediation film.It should
Preparation method is the following steps are included: S1, the physiology containing 0.5% calcium chloride by water soluble chitosan and Isin glue collagen active peptide
Salt water dissolution, is configured to solution A；Alginate is configured to solution B with physiological saline by S2；And S3, by solution A and solution B
It is blended in lyophilised state pushing film-like and obtains intrinsic bioremediation film.
Water soluble chitosan has good hemostatic and film forming, can effectively facilitate epithelial cell proliferation and inhibit into fibre
Tie up cell Proliferation, it is possible to provide good microenvironment promotes surface of a wound hemostasis, healing and repairs, and there are also certain antibacterial and anti-inflammation functions, can
Mitigate inflammatory reaction；Alginate will form flexible hydro-gel after absorbing body fluid, play the role of inflammatory exclusion, Wound protection；It adopts
The inflammatory reaction that Isin glue collagen active peptide can effectively facilitate mucosa regeneration reparation, inhibit ulcer wound surface, improves patient's
Compliance.The present invention is prepared after processing using water soluble chitosan, alginate, Isin glue collagen active peptide as main component
Intrinsic bioremediation film is obtained, can be used for canker sore etc..In the present invention, by being physical crosslinking, freezing extrusion forming technique, most
Finished product provides the film that form is mechanical property enhancing, easy to use and operate.
Preferably, the concentration of water soluble chitosan is 1.5~4wt% in solution A, and Isin glue collagen active peptide quality is water
The 0.01%~10.0% of soluble chitosan quality；It is furthermore preferred that after solution A and solution B mixing, water soluble chitosan and seaweed
The mass ratio of hydrochlorate is 1:1~10:1.
In order to improve the safety in utilization of intrinsic bioremediation film, the preparation of solution A and solution B aseptically into
Row；Preferably, further include the steps that carrying out irradiation sterilization to intrinsic bioremediation film after S3.
Beneficial effects of the present invention are further illustrated below in conjunction with embodiment.The reagent that do not write exactly in detail in the present invention
Or technological means, the conventional reagent or conventional technical means that this field can be used are realized, it can't be to the final result of experiment
Cause the influence with significant difference.
Embodiment 1
S1, take germ-free condition carboxymethyl chitosan 1.5g (molecular weight 200KDa, deacetylation 95%, degree of substitution 0.8),
Isin glue collagen active peptide 0.1g (molecular weight 3000Da) is dissolved in the sterile saline that 100ml contains 0.5% calcium chloride, preparation
Homogeneous solution A；
S2 takes the sodium alginate 1.5g (molecular weight 100KDa, M/G 1.2) of germ-free condition, is dissolved in 100ml physiological saline,
Prepare homogeneous solution B；
Under stirring well, solution A is slowly added into solution B by S3, and after mixing evenly, vacuum degassing bubble pours into pre-
Re-compacted at diaphragm after freeze-drying in cold mold, irradiation sterilization is after packaging to get intrinsic bioremediation film.
Above step carries out under sterile, clean state.
Embodiment 2
S1, take germ-free condition carboxymethyl chitosan 4.0g (molecular weight 250KDa, deacetylation 95%, degree of substitution 1.0),
Isin glue collagen active peptide 0.1g (molecular weight 3000Da) is dissolved in the sterile saline that 100ml contains 0.5% calcium chloride, preparation
Homogeneous solution A；
S2 takes the sodium alginate 0.4g (molecular weight 200KDa, M/G 1.2) of germ-free condition, is dissolved in 100ml physiological saline,
Prepare homogeneous solution B；
Under stirring well, solution A is slowly added into solution B by S3, and after mixing evenly, vacuum degassing bubble pours into pre-
Re-compacted at diaphragm after freeze-drying in cold mold, irradiation sterilization is after packaging to get intrinsic bioremediation film.
Above step carries out under sterile, clean state.
Embodiment 3
S1, take germ-free condition carboxymethyl chitosan 4.0g (molecular weight 250KDa, deacetylation 95%, degree of substitution 1.0),
Isin glue collagen active peptide 0.4g (molecular weight 2000Da) is dissolved in the sterile saline that 100ml contains 0.5% calcium chloride, preparation
Homogeneous solution A；
S2 takes the sodium alginate 4.0g (molecular weight 200KDa, M/G 1.5) of germ-free condition, is dissolved in 100ml physiological saline,
Prepare homogeneous solution B；
Under stirring well, solution A is slowly added into solution B by S3, and after mixing evenly, vacuum degassing bubble pours into pre-
Re-compacted at diaphragm after freeze-drying in cold mold, irradiation sterilization is after packaging to get intrinsic bioremediation film.
Above step carries out under sterile, clean state.
Embodiment 4
S1, take germ-free condition carboxymethyl chitosan 4.0g (molecular weight 250KDa, deacetylation 95%, degree of substitution 1.0),
Isin glue collagen active peptide 0.4g (molecular weight 2000Da) is dissolved in the sterile saline that 100ml contains 0.5% calcium chloride, preparation
Homogeneous solution A；
S2 takes the sodium alginate 2.0g (molecular weight 250KDa, M/G 1.2) of germ-free condition, is dissolved in 100ml physiological saline,
Prepare homogeneous solution B；
Under stirring well, solution A is slowly added into solution B by S3, and after mixing evenly, vacuum degassing bubble pours into pre-
Re-compacted at diaphragm after freeze-drying in cold mold, irradiation sterilization is after packaging to get intrinsic bioremediation film.
Above step carries out under sterile, clean state.
Embodiment 5
S1, take germ-free condition carboxymethyl chitosan 3.0g (molecular weight 300KDa, deacetylation 95%, degree of substitution 1.0),
Isin glue collagen active peptide 0.3g (molecular weight 2000Da) is dissolved in the sterile saline that 100ml contains 0.5% calcium chloride, preparation
Homogeneous solution A；
S2 takes the sodium alginate 2.0g (molecular weight 250KDa, M/G 1.5) of germ-free condition, is dissolved in 100ml physiological saline,
Prepare homogeneous solution B；
Under stirring well, solution A is slowly added into solution B by S3, and after mixing evenly, vacuum degassing bubble pours into pre-
Re-compacted at diaphragm after freeze-drying in cold mold, irradiation sterilization is after packaging to get intrinsic bioremediation film.
Above step carries out under sterile, clean state.
Embodiment 6
S1, take germ-free condition hydroxypropyl chitosan 3.0g (molecular weight 150KDa, deacetylation 95%, degree of substitution 0.8),
Isin glue collagen active peptide 0.2g (molecular weight 3000Da) is dissolved in the sterile saline that 100ml contains 0.5% calcium chloride, preparation
Homogeneous solution A；
S2 takes the sodium alginate 2.0g (molecular weight 100KDa, M/G 1.0) of germ-free condition, is dissolved in 100ml physiological saline,
Prepare homogeneous solution B；
Under stirring well, solution A is slowly added into solution B by S3, and after mixing evenly, vacuum degassing bubble pours into pre-
Re-compacted at diaphragm after freeze-drying in cold mold, irradiation sterilization is after packaging to get intrinsic bioremediation film.
Above step carries out under sterile, clean state.
Embodiment 7
S1, take germ-free condition chitosan lactate 3.0g (molecular weight 250KDa, deacetylation 95%, degree of substitution 1.0),
Isin glue collagen active peptide 0.3g (molecular weight 2000Da) is dissolved in the sterile saline that 100ml contains 0.5% calcium chloride, preparation
Homogeneous solution A；
S2 takes the potassium alginate 2.0g (molecular weight 250KDa, M/G 1.2) of germ-free condition, is dissolved in 100ml physiological saline,
Prepare homogeneous solution B；
Under stirring well, solution A is slowly added into solution B by S3, and after mixing evenly, vacuum degassing bubble pours into pre-
Re-compacted at diaphragm after freeze-drying in cold mold, irradiation sterilization is after packaging to get intrinsic bioremediation film.
Above step carries out under sterile, clean state.
Embodiment 8
S1, take germ-free condition hydroxypropyl chitosan 3.0g (molecular weight 250KDa, deacetylation 95%, degree of substitution 1.0),
Isin glue collagen active peptide 0.3g (molecular weight 2000Da) is dissolved in the sterile saline that 100ml contains 0.5% calcium chloride, preparation
Homogeneous solution A；
S2 takes the potassium alginate 2.0g (molecular weight 250KDa, M/G 1.2) of germ-free condition, is dissolved in 100ml physiological saline,
Prepare homogeneous solution B；
Under stirring well, solution A is slowly added into solution B by S3, and after mixing evenly, vacuum degassing bubble pours into pre-
Re-compacted at diaphragm after freeze-drying in cold mold, irradiation sterilization is after packaging to get intrinsic bioremediation film.
Above step carries out under sterile, clean state.
Embodiment 9
S1, take germ-free condition carboxymethyl chitosan 2.0g (molecular weight 250KDa, deacetylation 95%, degree of substitution 1.0),
Hydroxyethyl chitosan 2.0g (molecular weight 250KDa, deacetylation 95%, degree of substitution 1.0), Isin glue collagen active peptide 0.3g (divide
Son amount 2000Da), it is dissolved in the sterile saline that 100ml contains 0.5% calcium chloride, prepares homogeneous solution A；
S2 takes the sodium alginate 2.0g (molecular weight 250KDa, M/G 1.2) of germ-free condition, is dissolved in 100ml physiological saline,
Prepare homogeneous solution B；
Under stirring well, solution A is slowly added into solution B by S3, and after mixing evenly, vacuum degassing bubble pours into pre-
Re-compacted at diaphragm after freeze-drying in cold mold, irradiation sterilization is after packaging to get intrinsic bioremediation film.
Above step carries out under sterile, clean state.
Embodiment 10
S1, take germ-free condition carboxymethyl chitosan 2.0g (molecular weight 250KDa, deacetylation 95%, degree of substitution 1.0),
Hydroxyethyl chitosan 2.0g (molecular weight 250KDa, deacetylation 95%, degree of substitution 1.0), Isin glue collagen active peptide 0.3g (divide
Son amount 2000Da), it is dissolved in the sterile saline that 100ml contains 0.5% calcium chloride, prepares homogeneous solution A；
S2 takes the sodium alginate 2.0g (molecular weight 250KDa, M/G 1.2) of germ-free condition, is dissolved in 100ml physiological saline,
Prepare homogeneous solution B；
Under stirring well, solution A is slowly added into solution B by S3, and after mixing evenly, vacuum degassing bubble pours into pre-
Re-compacted at diaphragm after freeze-drying in cold mold, irradiation sterilization is after packaging to get intrinsic bioremediation film.
Above step carries out under sterile, clean state.
Embodiment 11
S1, take germ-free condition carboxymethyl chitosan 3.0g (molecular weight 250KDa, deacetylation 60%, degree of substitution 0.8),
Isin glue collagen active peptide 0.3g (molecular weight 2000Da) is dissolved in the sterile saline that 100ml contains 0.5% calcium chloride, preparation
Homogeneous solution A；
S2 takes the sodium alginate 2.0g (molecular weight 250KDa, M/G 1.5) of germ-free condition, is dissolved in 100ml physiological saline,
Prepare homogeneous solution B；
Under stirring well, solution A is slowly added into solution B by S3, and after mixing evenly, vacuum degassing bubble pours into pre-
Re-compacted at diaphragm after freeze-drying in cold mold, irradiation sterilization is after packaging to get intrinsic bioremediation film.
Above step carries out under sterile, clean state.
Embodiment 12
S1, take germ-free condition carboxymethyl chitosan 3.0g (molecular weight 250KDa, deacetylation 75%, degree of substitution 0.8),
Isin glue collagen active peptide 0.3g (molecular weight 2000Da) is dissolved in the sterile saline that 100ml contains 0.5% calcium chloride, preparation
Homogeneous solution A；
S2 takes the sodium alginate 2.0g (molecular weight 250KDa, M/G 1.5) of germ-free condition, is dissolved in 100ml physiological saline,
Prepare homogeneous solution B；
Under stirring well, solution A is slowly added into solution B by S3, and after mixing evenly, vacuum degassing bubble pours into pre-
Re-compacted at diaphragm after freeze-drying in cold mold, irradiation sterilization is after packaging to get intrinsic bioremediation film.
Above step carries out under sterile, clean state.
Embodiment 13
S1 takes carboxymethyl chitosan 3.0g (molecular weight 250KDa, deacetylation 75%, the degree of substitution of germ-free condition
0.85), Isin glue collagen active peptide 0.3g (molecular weight 2000Da) is dissolved in the sterile physiological salt that 100ml contains 0.5% calcium chloride
Water prepares homogeneous solution A；
S2 takes the sodium alginate 2.0g (molecular weight 250KDa, M/G 1.5) of germ-free condition, is dissolved in 100ml physiological saline,
Prepare homogeneous solution B；
Under stirring well, solution A is slowly added into solution B by S3, and after mixing evenly, vacuum degassing bubble pours into pre-
Re-compacted at diaphragm after freeze-drying in cold mold, irradiation sterilization is after packaging to get intrinsic bioremediation film.
Above step carries out under sterile, clean state.
Embodiment 14
S1 takes carboxymethyl chitosan 3.0g (molecular weight 250KDa, deacetylation 75%, the degree of substitution of germ-free condition
0.85), Isin glue collagen active peptide 0.3g (molecular weight 2000Da) is dissolved in the sterile physiological salt that 100ml contains 0.5% calcium chloride
Water prepares homogeneous solution A；
S2 takes the sodium alginate 2.0g (molecular weight 250KDa, M/G 1.5) of germ-free condition, is dissolved in 100ml physiological saline,
Prepare homogeneous solution B；
Under stirring well, solution A is slowly added into solution B by S3, and after mixing evenly, vacuum degassing bubble pours into pre-
Re-compacted at diaphragm after freeze-drying in cold mold, irradiation sterilization is after packaging to get intrinsic bioremediation film.
Above step carries out under sterile, clean state.
Embodiment 15
S1, take germ-free condition carboxymethyl chitosan 1.5g (molecular weight 200KDa, deacetylation 95%, degree of substitution 0.8),
Isin glue collagen active peptide 0.1g (molecular weight 3000Da) is dissolved in the sterile saline that 100ml contains 0.5% calcium chloride, preparation
Homogeneous solution A；
S2 takes the sodium alginate 1.5g (molecular weight 100KDa, M/G 1.0) of germ-free condition, is dissolved in 100ml physiological saline,
Prepare homogeneous solution B；
Under stirring well, solution A is slowly added into solution B by S3, and after mixing evenly, vacuum degassing bubble pours into pre-
Re-compacted at diaphragm after freeze-drying in cold mold, irradiation sterilization is after packaging to get intrinsic bioremediation film.
Above step carries out under sterile, clean state.
By the various embodiments described above compared with market sale watermelon crystal spray carries out effect, canker sore patient bursts sooner or later daily
Respectively paste a piece of at ulcer, 1 tablet once；Watermelon crystal spray by specification uses；Embodiment and comparative example respectively counts making for 40 patients
With effect, record such as the following table 1.
Table 1
Note: ulcer surface healing in 1 to 3 day is significant；Ulcer surface healing in 4 to 7 days is general；Ulcer surface, which heals, after 8 days is
It is inefficient.
It can be seen from the above description that the above embodiments of the present invention realized the following chievements:
1) intrinsic bioremediation film of the invention is by days such as water soluble chitosan, alginate and Isin glue collagen active peptides
Right bio-matrix composition, height bionic structure and function bionics, for can be changed into after ulcer wound surface hydrogel state attach in
Affected part forms moist barrier, reduces the friction with its hetero-organization or substance, and can promote ulcer wound surface healing, inhibits inflammatory anti-
Answer and reduce infection and other effects；
2) any drug or chemical reagent are not contained, it is living by the antibacterial of native biomatrix itself, anti-inflammatory and promoting healing
Property improve ulcer wound surface Regeneration and Repair situation and patient compliance, highly-safe, no drug abuse risk or chemical reagent
Remain intoxicating, teratogenesis risk；
3) by the mechanical property of physical crosslinking, freezing extrusion forming process improving wound repairing membrane, gained film has
Stronger mechanical property is easy to use operation；
4) water soluble chitosan, native biomatrix are contained only, without other any adding ingredients, cross contamination risk is low；Also
It is strong with process operability, the period is short, quality stability is good, the advantages such as low in cost；
5) formula is simple, does not introduce any other drug or ingredient, no drug resistance or allergy risk；It is with short production cycle,
Risk is controllable, low in cost, and quality is stablized.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (13)
1. a kind of intrinsic bioremediation film, which is characterized in that raw material is living by water soluble chitosan, alginate, Isin glue collagen
Property peptide and auxiliary material composition.
2. intrinsic bioremediation film according to claim 1, which is characterized in that the water soluble chitosan and the seaweed
The mass ratio of hydrochlorate is 1:1~10:1, and the Isin glue collagen active peptide quality is the water soluble chitosan quality
0.01%~10.0%.
3. intrinsic bioremediation film according to claim 1, which is characterized in that the molecular weight of the water soluble chitosan >=
150KDa, deacetylation 60~98%, degree of substitution >=0.8；Molecular weight >=100KDa of the alginate, M/G >=1.0；Institute
State molecular weight≤3000Da of Isin glue collagen active peptide.
4. intrinsic bioremediation film according to claim 1, which is characterized in that the water soluble chitosan is selected from by carboxylic first
Base enclosure glycan/chitin, hydroxypropyl chitosan/chitin, hydroxyethyl chitosan/chitin, hydroxyl butyl chitosan/chitin,
One of group that chitosan quaternary ammonium salt, chitosan lactate, glutamate and sulfated chitosan form is a variety of.
5. intrinsic bioremediation film according to claim 1, which is characterized in that the alginate is selected from by alginic acid
One of group of sodium, potassium alginate and ammonium alginate composition is a variety of.
6. intrinsic bioremediation film according to claim 1, which is characterized in that the Isin glue collagen active peptide derives from
The fish-skin of marine fishes or freshwater fish, fish scale or fish-bone.
7. intrinsic bioremediation film according to claim 3, which is characterized in that the molecular weight of the water soluble chitosan is big
It is less than or equal to 500KDa, deacetylation 75~95%, degree of substitution >=0.85 in being equal to 200KDa；The molecular weight of the alginate
It is less than or equal to 500KDa, M/G >=1.2 more than or equal to 100KDa；Molecular weight≤3000Da of the Isin glue collagen active peptide.
8. intrinsic bioremediation film according to claim 2, which is characterized in that the water soluble chitosan and the seaweed
The mass ratio of hydrochlorate is 3:2~5:1.
9. intrinsic bioremediation film according to any one of claim 1 to 8, which is characterized in that the natural biological is repaired
Laminating adhesive is applied to canker sore.
10. a kind of preparation method of intrinsic bioremediation film as claimed in any one of claims 1-9 wherein, which is characterized in that packet
Include following steps: a kind of canker sore intrinsic bioremediation film, preparation include the following steps:
S1, the physiological saline solution containing 0.5% calcium chloride by water soluble chitosan and Isin glue collagen active peptide, is configured to molten
Liquid A；
Alginate is configured to solution B with physiological saline by S2；And
The solution A and the solution B are blended in lyophilised state pushing film-like and obtain the intrinsic bioremediation film by S3.
11. preparation method according to claim 10, which is characterized in that water soluble chitosan described in the solution A
Concentration be 1.5~4wt%, the Isin glue collagen active peptide quality be the water soluble chitosan quality 0.01%~
10.0%.
12. preparation method according to claim 10, which is characterized in that after the solution A and solution B mixing, institute
The mass ratio for stating water soluble chitosan and the alginate is 1:1~10:1.
13. preparation method according to claim 10, which is characterized in that the preparation of the solution A and the solution B exists
It is carried out under aseptic condition；Preferably, further include the steps that carrying out irradiation sterilization to the intrinsic bioremediation film after the S3.
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