CN109845638A

CN109845638A - A kind of storage tolerance tomato selection

Info

Publication number: CN109845638A
Application number: CN201910036280.4A
Authority: CN
Inventors: 宰文珊; 马彦如; 叶曙光; 史建磊; 熊自立; 张海利
Original assignee: Wenzhou Polytechnic
Current assignee: Wenzhou Polytechnic
Priority date: 2019-01-15
Filing date: 2019-01-15
Publication date: 2019-06-07

Abstract

The present invention relates to tomato breeding fields, and in particular to a kind of storage tolerance tomato selection, comprising the following steps: (1) is introduced, be continuously selfed, the tomato resources of storage tolerance are isolated in screening by seed collection, branch acquisition；(2) carry out hybridization apolegamy for screening isolated material, in combination with store index it is relatively high be it is maternal, hybridized；(3) first filial generation after hybridization is cultivated, picks fruit, arrange to carry out storage test.By hybridization research to tomato and storage test, for discovery storage index with maternal related, the high first filial generation of female parent storage index is corresponding also higher.So being hybridized in Breeding Process with storing the high female parent of index, the higher first filial generation of available storage index.

Description

A kind of storage tolerance tomato selection

Technical field

The present invention relates to tomato breeding fields, and in particular to a kind of storage tolerance tomato selection.

Background technique

Tomato is the second largest industrial crops in China, is only second to cucumber.China region is wide, and north-south climate difference is big, thus makes At the seasonality of tomato production and the anniversary harmony contradiction of demand.Cause dull season insufficient supply, price high, and in busy season tomato Listing is concentrated, and drug on the market, rots.Loss is up to 50% after adopting every year according to Luo Yun wave report tomato, only the annual tomato in Xinjiang Postharvest decay loses just at 40,000,000 yuan or more.How balanced tomato market supply, reduce tomato and rot loss, be that China is current The major subjects of tomato research.About 40,000 mu of Wenzhou City's tomato cultivation area, this several years a large amount of introduction Israel, Holland, France etc. The tomato variety of country, because import variety yield is high, quality is good, storage tolerance, promotes quickly in the city.But external variety seeds Price is very expensive, and peasant planting cost is big, simultaneously because the difference of region and corresponding cultivation techniques, plants external kind risk Also big.And the kind of dependence foreign countries simply is also unfavorable for the development of tomato industry.According to producers and consumers to tomato The requirement of kind, we want positive breeding storage tolerance New Tomato Variety.

Summary of the invention

The purpose of the invention is to overcome shortcoming and defect of the existing technology, and provide a kind of storage tolerance tomato choosing Educate method.

The technical solution used in the present invention is as follows: a kind of storage tolerance tomato selection, comprising the following steps:

(1) it introduces that Comprehensive Traits are excellent and material containing slow ripe gene mutation body by seed collection, branch acquisition, carries out The tomato resources of storage tolerance are isolated in continuous selfing, screening；

(2) hybridization apolegamy is carried out for screening isolated material, it is relatively high for female parent to store index in combination, it stores Index is relatively low for male parent, is hybridized, obtains storage tolerance tomato；

(3) first filial generation after hybridization is cultivated, picks fruit, arrange to carry out storage test.

In step (2), the first filial generation after hybridization is cultivated, random sampling, investigation plant biology are carried out during cultivation Shape, Fruit Characteristics are learned, and counts commodity fruit yield in harvesting latter stage, following kind is screened out: single fruit weight being 1. less than 180g Kind；2. the ratio that the quantity of defective, dehiscent fruit or empty fruit accounts for the total number of fruits by fruit face is greater than 10% kind.

In step (1), it is as follows to screen the yellow fruit resource isolated:

Lima company, 720-4-2 strain material source France forms, major traits through continuous 8 generation selfed breeding are as follows: unlimited Growth type, growth potential are strong；Dark green leaf, fruit setting power are strong；Calyx is big calyx, calyx gauffer；Fruit circle, shoulder of having no result are green Fruit is white, and mellow fruit is yellow fruit, and fruit is hard, stores without putrefaction in six months at room temperature；3~4 ventricle of fruit, pulp are averaged thickness 10mm, 200~240 grams of single fruit weight；

Lima company, 720-6-1-11 strain material source France forms, major traits through continuous 11 generation selfed breeding are as follows: Indeterminate growth type, plant type is sparse, dark green leaf, and fruit setting power is strong；Fruit circle, shoulder of having no result, Chinese olive is white, and mellow fruit is yellow fruit, Fruit is hard；3~4 ventricle of fruit, pulp are averaged thickness 8.5mm, and 170 grams of single fruit weight or so；

Lima company, 720-9-1-6 strain material source France forms, major traits through continuous 11 generation selfed breeding are as follows: Resisting etiolation leaf curl viral disease；Indeterminate growth type, growth potential is medium, dark green leaf, and fruit circle, shoulder of having no result, Chinese olive is white, mature Fruit is yellow fruit, and fruit is hard；3~4 ventricle of fruit, pulp are averaged thickness 9.5mm, and 160 grams of single fruit weight or so；

Lima company, 716-2-2-15 strain material source France forms, major traits through continuous 8 generation selfed breeding are as follows: Indeterminate growth type, growth potential is strong, dark green leaf, and fruit setting power is strong；Calyx is big calyx, spray acropetal leaf；Fruit circle is slightly flat, Fruit surface smooth, fruit type is good, and shoulder of having no result, Chinese olive is white, and mellow fruit is yellow fruit, and single fruit is big, and fruit is hard；2~4 ventricle of fruit, pulp are flat Equal thickness 10mm, 200~220 grams of single fruit weight；

712 Lima companies, short Huang -1-6 strain material source France, form, major traits through continuous 8 generation selfed breeding are as follows: Determinate growth type, growth potential are strong；Blade is big, potato blade profile, light green color, and flowering fruit is good under low temperature；Calyx is big calyx, fruit Real circle, Chinese olive is white, shoulder of having no result, mature fruits yellow；Fruit sepal is big, light green color, the oversensitive room of fruit, firmly, average fruit kind 220 grams；

Lima company, 78-3-1-11 strain material source France forms through continuous 8 generation selfed breeding；Major traits are as follows: nothing Growth type is limited, growth potential is strong, dark green leaf, and fruit setting power is strong；Fruit is oblate, and fruit type is good, and mellow fruit is yellow fruit, and fruit is hard； 3~5 ventricle of fruit, pulp are averaged thickness 6.5mm, and 200~210 grams of single fruit weight；

The parent material number part matched therewith, the feature of main material are filtered out simultaneously are as follows: 711-2-35-19 strain Lima company, material source France forms through continuous 11 generation selfed breeding；Major traits are as follows: resisting etiolation leaf curl viral disease；Infinitely Growth type, plant type is sparse, and cold resistance is good, dark green leaf, and calyx is more open and more flat；Fruit circle is slightly flat, and light fruit shoulder, Chinese olive is green, Mature fruits large red, uniform coloring, pulp is red, and pulp is averaged thickness 6.5mm, 3~4 ventricle of fruit, mean fruit weight 150 Gram；

3-22-2-1 strain material source Israel Hai Zela is formed by FA189 through continuous 13 generation selfed breeding；Main property Shape are as follows: indeterminate growth type, growth is medium, and cold resistance is good, and fruit setting power is strong；Fruit circle is slightly flat, and light fruit shoulder, Chinese olive is green, mature Fruits large red, uniform coloring；Fruit sepal is elongated, and bottle green, carpopodium is short, and inflorescence is unfolded；Every Honoka florescence neat and consistent, 3~4 flowers of every Honoka sequence；4~5 ventricle of fruit, 220 grams of mean fruit weight.

The tomato of first filial generation, which is cultivated, can use soil cultivation, soilless cultivation two ways.

Preferably, in step (2), using soil cultivation, decomposed dung 800kg, calcium magnesium phosphate 50kg are experimental field applied per acre, Ternary compound fertilizer is base manure, and entire breeding time top dressing 4 times, accumulative mu imposes urea 60kg, ternary compound fertilizer 50kg, potassium chloride 60kg is experimental field covered using fly net, carries out repeatedly spray prevention and treatment to Bemisia tabaci during test.

Preferably, in step (2), using soilless cultivation, in sowing the latter moon seedling separation, another is colonized in cultivation after the moon Slot, cultivation bed are made of cystosepiment, layer overlay silver gray plastic foil and open aperture by 0.8m spacing in slot, after fill out to send out Ferment sawdust is the mixed-matrix of primary raw material, uses nutrient fluid drip irrigation in cultivation, tests and uses RANDOMIZED BLOCK DESIGN, 2 times Repeat, every cell plants 92 plants of tomato, spacing in the rows 0.20m, using Netted melon, the oblique hanging tendril of lanyard, odd number strain unify lanyard in Cultivation bed upper left side, even number strain unify lanyard in cultivation bed upper right side, promote fruit setting using drop-proof agent spraying decoration, test greenhouse surrounding It is experimental field interior to hang Yellow sticky trap at a certain distance using 30 mesh fly net insect prevention, carry out 3 sprays during test altogether to Bemisia tabaci Medicine prevention and treatment.

Above two mode respectively has advantage and disadvantage, and first filial generation quantity type is more after hybridization, preferably soil cultivation, cost compared with Low, management is simple, can screen out the kind that low output, fruit are small, commodity is low, after hybridization first filial generation quantity type compared with It is few, or after screening out part kind by soil cultivation, by soilless cultivation, convenient for management, effective control of environmental factor, Storage test is finally carried out, the optimal kind of storage performance is selected, comparative analysis is more scientific.

In step (3), storage test can be carried out using following two mode:

In the harvesting later period, in experimental plot, the fruit of annesl, no disease and pests harm and mechanical damage is completed in 90% or more fruit face of picking Real, each fruit picking 100 of each kind arranges to carry out room temperature storage test, and storage period room temperature is 20 DEG C ± 5 DEG C, and humidity is 80% ± 5%, the fruit for losing commodity is recorded and rejected daily, until each kind healthy fruit is reduced to 50% or so end storage examination It tests, and counts storage period number of days and bad fruit type and ratio.

In harvesting mid-term, taken out of each cell respectively disease-free, neat and consistent tamato fruit several, be put into plastic crate It is interior, it impregnates to take out after five minutes through 2.5% sodium hypochlorite and dry, be placed on 25 DEG C under room temperature, it is primary every investigation in 10 days, it goes Except the fruit for losing commodity value, green fruit and red fruit healthy fruit and storage index are calculated.

Beneficial effects of the present invention are as follows: by hybridization research to tomato and storage test, discovery storage index is with mother This is related, and the high first filial generation of female parent storage index is corresponding also higher.So in Breeding Process, with store index it is high it is maternal into Row hybridization, the higher first filial generation of available storage index.

Specific embodiment

(1) Tomato Germplasm Resources collect the screening with storage tolerance material

It is excellent and containing slow ripe gene mutation body from some Comprehensive Traits are introduced both at home and abroad by seed collection, branch acquisition Nearly more than 80 part of material is created the long tomato breeding material of storage tolerance, shelf life using various methods, is passed through for these materials Inhereditary feature assessment identification (economical character, fruit properties), filters out 6 parts of parent material of storage tolerance, main feature characteristic Have:

Lima company, 720-4-2 strain material source France forms through continuous 8 generation selfed breeding.Major traits are as follows: unlimited Growth type, growth potential are strong；Dark green leaf, fruit setting power are strong；Calyx is big calyx, calyx gauffer.Fruit circle, shoulder of having no result are green Fruit is white, and mellow fruit is yellow fruit, and fruit is hard, stores without putrefaction in six months at room temperature；3~4 ventricle of fruit, pulp are averaged thickness 10mm, 200~240 grams of single fruit weight；

Lima company, 720-6-1-11 strain material source France forms through continuous 11 generation selfed breeding.Major traits are as follows: Indeterminate growth type, plant type is sparse, dark green leaf, and fruit setting power is strong；Fruit circle, shoulder of having no result, Chinese olive is white, and mellow fruit is yellow fruit, Fruit is hard；3~4 ventricle of fruit, pulp are averaged thickness 8.5mm, and 170 grams of single fruit weight or so；

Lima company, 720-9-1-6 strain material source France forms through continuous 11 generation selfed breeding.Major traits are as follows: Resisting etiolation leaf curl viral disease；Indeterminate growth type, growth potential is medium, dark green leaf, and fruit circle, shoulder of having no result, Chinese olive is white, mature Fruit is yellow fruit, and fruit is hard；3~4 ventricle of fruit, pulp are averaged thickness 9.5mm, and 160 grams of single fruit weight or so；

Lima company, 716-2-2-15 strain material source France forms through continuous 8 generation selfed breeding.Major traits are as follows: Indeterminate growth type, growth potential is strong, dark green leaf, and fruit setting power is strong；Calyx is big calyx, spray acropetal leaf.Fruit circle is slightly flat, Fruit surface smooth, fruit type is good, and shoulder of having no result, Chinese olive is white, and mellow fruit is yellow fruit, and single fruit is big, and fruit is hard；2~4 ventricle of fruit, pulp are flat Equal thickness 10mm, 200~220 grams of single fruit weight.

712 Lima companies, short Huang -1-6 strain material source France, form through continuous 8 generation selfed breeding.Major traits are as follows: Determinate growth type, growth potential are strong；Blade is big, potato blade profile, light green color, and flowering fruit is good under low temperature；Calyx is big calyx, fruit Real circle, Chinese olive is white, shoulder of having no result, mature fruits yellow；Fruit sepal is big, light green color, the oversensitive room of fruit, firmly, average fruit kind 220 grams.

Lima company, 78-3-1-11 strain material source France forms through continuous 8 generation selfed breeding.Major traits are as follows: nothing Growth type is limited, growth potential is strong, dark green leaf, and fruit setting power is strong；Fruit is oblate, and fruit type is good, and mellow fruit is yellow fruit, and fruit is hard； 3~5 ventricle of fruit, pulp are averaged thickness 6.5mm, and 200~210 grams of single fruit weight.

The parent material number part matched therewith, the feature of main material are filtered out simultaneously are as follows:

Lima company, 711-2-35-19 strain material source France forms through continuous 11 generation selfed breeding.Major traits Are as follows: resisting etiolation leaf curl viral disease；Indeterminate growth type, plant type is sparse, and cold resistance is good, dark green leaf, and calyx is more open and more flat；Fruit Round slightly flat, light fruit shoulder, Chinese olive is green, and mature fruits large red, uniform coloring, pulp is red, and pulp is averaged thickness 6.5mm, fruit 3 ~4 ventricles, 150 grams of mean fruit weight.

3-22-2-1 strain material source Israel Hai Zela is formed by FA189 through continuous 13 generation selfed breeding.Main property Shape are as follows: indeterminate growth type, growth is medium, and cold resistance is good, and fruit setting power is strong；Fruit circle is slightly flat, and light fruit shoulder, Chinese olive is green, mature Fruits large red, uniform coloring；Fruit sepal is elongated, and bottle green, carpopodium is short, and inflorescence is unfolded；Every Honoka florescence neat and consistent, 3~4 flowers of every Honoka sequence；4~5 ventricle of fruit, 220 grams of mean fruit weight.

(2) hybridization apolegamy and Combination nova field variety comparative test

Material preferable for the performance of screening carries out hybridization apolegamy, and the second half year in 2007 configures combination altogether 21, compiles Number it is respectively as follows: 801 (720-7-1 × 6-13-5), 802 (716-6-3 × 6-13-5), 804 (540-1-2 × 720-4-2), 805- 1 (625-1-4 × 720-4-2), 805-2 (720-8-2 × 627-5-1), 806-1 (711-2-35 × 720-6-1), 806-2 (720-6-1 × 711-2-35), 806-3 (720-4-10 × 711-2-35), 807 (78-3-1 × 720-4-2), 808 (543- 22-2-1 × 720-4-2), 809 (6-13-5 × 720-4-2), 813 (711-2-32 × 151-2-1), 814 (543-3-1 × 78- 3-1), 815 (625-1-4 × 78-3-1), 816 (543-2-1 × 711-2-24), 817 (716-6-3 × 151-2-1), 820 (151-2-1 × 711-2-35), 821 (627-5-1 × 711-2-35), 822 (712-3-6 × 627-5-1), 824 (720-4-3 Red × 711-2-35), 825 (543-3-1 × 711-2-35).Development field Comprehensive Traits sieve in 2008 is combined in for apolegamy Choosing.Test arrangement carries out in this institute, vegetables institute, is control with FA189.It is sowed July 18 current year, unified transplanting in August 20th Field planting, test are repeated 2 times repetition, and every combination and kind are colonized 54 plants, experimental field apply decomposed dung 800kg, calcium magnesium phosphate per acre 50kg, ternary compound fertilizer are base manure, and entire breeding time top dressing 4 times, accumulative mu imposes urea 60kg, ternary compound fertilizer 50kg, chlorine Change potassium 60kg.It is experimental field covered using fly net, repeatedly spray prevention and treatment is carried out to Bemisia tabaci during test, other management are the same as normal Rule.During test 10 plants of random sampling in each combination and kind, plant biological character, Fruit Characteristics are investigated, and in harvesting Each combination of latter stage (April 15 next year) statistics or kind cell commodity fruit yield, concrete condition are as follows:

(1) plant biological character compares

1 plant biological character of table compares

(tomato yellow leaf curl China viral disease being occurred for part, and more serious combination of falling ill, do not count in table)

From table 1, each Combination nova plant growing way compared with FA189 is relatively strong, and special 808 growth potential is stronger.Each Combination nova Make comparisons with FA189, in addition to 806-1,806-2,806-3,804 begin that beginning fruiting period is spent to do sth. in advance 3~4d, remaining each combination substantially with FA189 is suitable.In terms of inflorescence spacing, 821 spacing of combination will obviously be grown compared with FA189, and 813,815 spacing of combination are obvious compared with FA189 Want short, remaining combination difference is unobvious.1~3 grade of fruit setting sum of tomato single plant is investigated, except combination 813,821,804,805-1 ratio FA189 wants low outer, remaining Combination nova and FA189 difference are unobvious.

(2) Fruit Characteristics and commodity fruit Yield comparison

As shown in Table 2, it respectively participates in the experiment combination or kind fruit shape is had nothing in common with each other, but fruit setting uniformity, annesl are preferable, and fruit shoulder Thin or shoulder of having no result.In terms of single fruit weight, combine 806-1,806-3,821,804,803 in 180g hereinafter, be middle fruitlet, remaining Combination be in big fruit, easily for market receive.Part combination (813,821,803,815) fruit face is defective or there are more dehiscent fruits Or empty fruit phenomenon, the commodity of strong influence fruit.In terms of hardness of fruit test value, 806-2,806-3,808 fruit Hardness is more apparent to be better than other combinations and kind.Compare cell commodity fruit yield, obviously more other groups of 808,806-2 and 805-2 Conjunction wants high, and FA189 commodity fruit yield is minimum, and main cause is related with TLCV disease incidence.

2 Fruit Characteristics of table and commodity fruit Yield comparison

By combining each character to participating in the experiment and being comprehensively compared, preliminary screening goes out general performance preferable 808, and (805-2 is 808 reciprocal cross materials) and two combinations of 806-2 (the reciprocal cross material that 806-1 is 806-2), it samples and surveys on the basis of screening Fixed 808,806-2 and control FA189 quality.

(3) fruit quality measures

Known (experimental data is provided by central laboratory, Zhejiang Prov. Inst. of Subtropical Crops) by table 3, three kinds, fruit contains Water is suitable；806 content highest of soluble solid, FA189 content is minimum, and 808 fall between, but three's content difference away from It is smaller；Total acid, soluble sugar content are suitable, and 806 content highest of Vc content, 808 contents are minimum, FA189 between 806,808 it Between；The hardness of fruit 808 is most hard, and FA189 is most soft, and 806 between 808 and FA189.

3 Tomato Quality of table measures (average ± standard error)

The different English alphabets marked after identical index show the significance level of difference (p < 0.05) of the two

It can be seen that three kinds from field variety comparative test and quality determination data to be wanting in quality away from little, but in field Between show and respectively have feature, bowl show 806 is precocious compared to control, and disease resistance is good, and bowl show 808 is compared to control yielding ability, and later period table It is now good.But from the point of view of character of bearing fruit, what is behaved oneself best is still control FA189, but FA189 variable rate technology is easily susceptible.

2, preferred kind variety comparative test

By above-mentioned test, preferably 808,806 two kinds, comparison kind are FA189 (CK1) (by Israel Hai Ze out Drawing company for kind), again be full of (CK2) (by Syngenta Co., Ltd, Switzerland for kind).

Test site is located in company, vegetables institute, academy of agricultural sciences, Wenzhou City vinyl house, and test is carried out using Soilless Culture Methods (mainly overcoming the possible bacterial wilt of soil many years sequential cropping cultivation, wilt disease harm), (slot is made using cystosepiment in cultivation bed Wide 0.28m, groove depth 0.15m), layer overlay silver gray plastic foil and open aperture by 0.8m spacing in slot, after fill out with the saw that ferments End is the mixed-matrix of primary raw material, (is matched with the chemical plant soilless cultivation of Hangzhou, Zhejiang province Longshan in cultivation using nutrient solution Square fertilizer dilution is made) trickle irrigation.Test uses RANDOMIZED BLOCK DESIGN, 2 repetitions, and every cell plants 92 plants of tomato, spacing in the rows 0.20m.It tests and is sowed July 20 next year, be divided into seedlings within next year August 10th, be colonized in cultivation bed within next year September 10th, using list Bar training, the oblique hanging tendril of lanyard, odd number strain unify lanyard in cultivation bed upper left side, and even number strain unifies lanyard in cultivation bed upper right Side promotes fruit setting using drop-proof agent spraying decoration.It tests greenhouse surrounding and uses 30 mesh fly net insect prevention, it is experimental field interior to hang at a certain distance Yellow sticky trap carries out 3 spray prevention and treatments altogether to Bemisia tabaci during test, other management are the same as conventional.

Sowing time, the setting date of tomato are recorded during test, the early flowering season and receipts phase fruit beginning of each kind, meter are recorded in observation It calculates each kind and is seeded into receipts phase beginning total number of days.

It is sample that test early period, which randomly selects 10 plants of plant in the every cell of each kind, observes the 1st inflorescence section and height, system Count that 1-5 inflorescence average headway, taking the 5th inflorescence of sample plant, its leaf width of each Blade measuring and leaf are long up and down, to plant Growth potential and cold resistance carry out sensory evaluation.

Each kind Chinese olive fruit shoulder is observed during test, sense organ is carried out to fruit annesl speed and annesl degree and is commented Valence is observed fruit table feature, and it is that sample is listed that test early period, which randomly selects 10 plants of plant in each cell of each kind, The malformed fruits rates such as navel corruption fruit, dehiscent fruit, empty fruit are counted during test；Test mid-term take the 5th grade of inflorescence of each kind sample plant at Ripe commodity fruit carries out single fruit weight, longitudinal and transverse demeter ratio, pulp thickness, the hardness of fruit and weighs or measure.

In on March 1, in third, in above-mentioned variety comparative test field, annesl, no disease and pests harm and machine are completed in 90% or more fruit face of picking The fruit of tool damage, each fruit picking 100 of each kind arrange to carry out room temperature storage test.Storage period room temperature is 20 DEG C ± 5 DEG C, humidity is 80% ± 5%, records daily and rejects the fruit (also known as bad fruit) for losing commodity, until each kind healthy fruit is (again Claim commodity fruit rate) it is reduced to 50% or so end storage test, and count storage period number of days and bad fruit type and ratio.

Three different times statistics tomato yields and the output value before, during and after this test is pressed, early period be on November next year 10 extremely December 31 next year, mid-term are 20 days 2 months 1 day January in third year to third year, and later period yield is 21 days 2 months third year to third On April 10, in.

(1) breeding time compares

The breeding time of each tested variety is investigated, the results are shown in Table 4.In terms of the flower situation that begins, 806F₁、808F₁Earliest, FA189 secondly, be full of at the latest again；In terms of receipts phase beginning, 806F₁Earliest, 808F₁, FA189 secondly, be full of again relatively late.

4 breeding time of table compares

(2) plant biological characteristics compare

5 plant biological characteristics of table compare

In terms of the 1st inflorescence section, the 1st inflorescence height, inflorescence average headway (see Table 5 for details), it is full of again in each tested variety Every numerical value shows minimum, illustrate the kind yield positive results position distribution it is compact, have typical yielding ability feature；806F₁ It is substantially close with every numerical value of FA189, and 808F₁Every numerical value is slightly biased big.In terms of blade profile, 808F₁Length of blade is most short, 806F₁Width of blade is most narrow, and the blade that is full of again is most wide.The early growth gesture of each tested variety is relatively strong, but with flowering fruit section The increase of position removes bowl show 808F₁Growth potential is still strong outer, remaining 3 kind growth potential gradually dies down.Each kind plant of participating in the experiment is cold-resistant Property it is relatively strong.

(3) fruit properties compare

(6 are shown in Table) in terms of fruit appearance, and each kind of participating in the experiment is oblateness；It is full of again, FA189 kind is without Chinese olive shoulder, annesl Fastly, color is scarlet after annesl, and fruit mass color is sliding without rib, 808F₁And 806F₁There is fruit shoulder, annesl is very fast, is in large red, fruit table after annesl Slightly rib ditch.In terms of single fruit weight, it is full of maximum again, 808F₁With FA189 secondly, 806F₁It is minimum.In terms of hardness of fruit measured value, 808F₁And 806F₁It is full of compared with FA189, again big, field statistics discovery FA189 and is full of navel corruption fruit rate and Cracking fruit again compared with 808F₁ And 806F₁Height, 806F₁Empty fruit rate is minimum.

6 fruit properties of table compare

(4) sex investigation is stored

Test result shows and (is shown in Table 7) 808F₁、806F₁Storage keeps the storage number of days point of 100% commodity fruit at room temperature Not Wei 11 days, 10 days, and FA189 and be full of again only have 9 days；The storage number of days for keeping 50% commodity fruit, be full of again, FA189, 806F₁、808F₁Respectively 24 days, 26 days, 28 days and 30 days.It can be seen that 808F₁、806F₁Keeping quality be better than FA189 and It is full of kind again.

Table 7 stores sex investigation

(6) yield and the output value compare

8 yield of table and the output value compare

Note: early yield is 10 days November next year to fruit picking per mu yield in 31 days December next year, 2.8 yuan/kg of price；Mid-term produces Amount is 20 days 2 months 1 day January in third year to third year fruit picking per mu yield, 3.4 yuan/kg of price；Later period yield is 2 months 21 third year Day to fruit picking per mu yield in 10 days April in third year, 3.0 yuan/kg of price.

This test fruit picking since 12 days November next year, until on April in third year 10 stopped adopting, three periods before, during and after pressing Statistics yield simultaneously calculates the output value, and three period product market price are shown in Table 8 notes.Test result shows: FA189 yield and the output value are most It is low, it is full of again secondly, 806F₁And 808F₁Yield and output value highest.

(3) parent material and combinations thereof storage property, quality trait research

Keeping quality test and nutrition Quality Analysis are carried out to 806,808 combined female parents, male parent and reciprocal cross material, with Theoretical foundation is provided for reasonable utilize of slow ripe genetic material.

Test material is that label is that it is 3- that 720-4-2 (808 male parent) is marked later to 3-22-2 (808 is maternal) later 22-2 × 720-4-2 (808 orthotropic material) is marked later, 720-4-2 × 3-22-2 (808 reciprocal cross material) mark later for F2；Label is that it is 720-6-1 × 711-2- that 711-2-35 (806 male parent) is marked later to 720-6-1 (806 is maternal) later 35 (806 orthotropic materials) mark later, and 711-2-35 × 720-6-1 (806 reciprocal cross material) is marked later is.Material in It sows within August 21 days, is divided into seedlings within September 10th, was colonized in Wenzhou Academy of Agricultural Sciences Vegetable Research Institute greenhouse on October 6, soil cultivation, often Part material plants a cell, 30 plants of every cell, and spacing in the rows 0.4m bears fruit naturally, remaining production management by convention.

It stores the measurement of index: taking disease-free, neat and consistent tomato out of each cell respectively in 1 year 19 days 1 month year Fruit several, be put into plastic crate, through 2.5% sodium hypochlorite impregnate after five minutes take out dry, be placed on 25 DEG C of room temperature conditions Under.Primary every investigation in 10 days, removal loses the fruit (shrinkage of fruit face is faint in color, rots) of commodity value, and it is (green to calculate green fruit Ripe phase, veraison) and red fruit (pink phase, red ripe phase) healthy fruit and storage index,

Hardness of fruit measurement: each material takes veraison, red ripe period two-phase fruit measurement respectively.Around fruit equator Portion, it is equidistant to take 3 point GY-1 type fruit hardness meters.Every material measures 6 fruits, 3 repetitions every time.

The measurement of capacitive solid content: first being returned to zero hand refractometer with clear water, take the tomato pulp of different parts, is squeezed For juice drop on refractometer, juice covers entire smooth surface, is repeated three times.It varies to be cleaned smooth surface with clear water before product and wipe It is dry.The soluble solid content of red ripe phase and veraison tomato are measured respectively.

The measurement of Soluble adhesion molecule: it is measured using anthrone method.

Vitamin C content measurement: it is measured using 2,4 dinitrophenyl hydrazine colorimetric method.

The measurement of lycopene content: taking tamato fruit, is ground into homogenate, fills 10ml centrifuge tube, in 6000r/min from The heart 20 minutes.Residue 1.0000g is taken, acetone 5ml, petroleum ether 5ml are added, mixing is poured into 50ml conical flask, stirred with glass bar Uniformly, it covers, is shaken 1 hour on shaking table.It takes clarified solution in 15ml centrifuge tube, adds 5ml distilled water, shake up, in 4000r/min Centrifugation 10 minutes, takes supernatant in 10ml volumetric flask, is settled to 10ml with petroleum ether, which is prepare liquid.

1, the specificity analysis of resistance to storage

(1) index is stored

As can be seen from Table 9, healthy fruit is higher when green fruit stores 20 days, minimum 69.23；At 40 days, in addition to P4 and P14, He keeps higher healthy fruit at kind；At 50 days, P2, F12 and P3 still keep higher healthy fruit.Pass through chi-square analysis, storage 40 days or 50 days, the green fruit healthy fruit of different cultivars had extremely significant difference.With the growth of storage time, green fruit healthy fruit by Decrescence small, wherein the green fruit healthy fruit of P4 is minimum, P2, F12 and P3 higher.When storing 40 days, F12 healthy fruit is maximum, and P4 is good Fruit rate is minimum；When storing 50 days, P2 is maximum, and P4 is minimum, and P4 fruit all loses commodity value.

The green fruit healthy fruit of the different tomatoes of table 9, storage index variation and annesl situation

Table9 Comparison Among the Green Tomato Fruit in Good Quality Percentage,ASI and Color Development.

The different tomato red fruit healthy fruits of table 10, storage index variation

Table 10Comparison Among the red Tomato Fruit in Good Quality Percentage and ASI.

As can be seen from Table 10, in addition to P4, other tomato healthy fruits are higher when red fruit is stored 20 days；At 40 days, various tomatoes Healthy fruit significantly reduces, and up to P1 is worth and all loses commodity value for 45.45, P4；At 50 days, healthy fruit continues to reduce, most High level only has 13.64, P2 and F13 healthy fruit is 0.By chi-square analysis, store 20 days, 40 days or 50 days, different cultivars it is red Fruit healthy fruit has extremely significant difference.With the growth of storage time, green fruit healthy fruit is gradually reduced, wherein the green fruit healthy fruit of P4 It is minimum, P2, F12 and P3 higher.When storing 40 days, F12 healthy fruit is maximum, and P4 healthy fruit is minimum；When storing 50 days, P2 is most Greatly, P4 is minimum, and P4 fruit all loses commodity value.From the healthy fruit changing rule of red fruit, P4 fruit shelf can be speculated Phase is within 20 days, and P1, P2, F11, F13, F14 fruit shelf life are between 20-40 days.

Test result shows the green fruit storage index of different cultivars tomato there are significant difference, after storage 50 days, except F1 with Outside P4, other kinds storage index is all very high, reaches as high as 98, this illustrates that the storage property of the green fruit of most of kinds is very good.Respectively It is respectively P2, F2, P3, F3, P1, F1, F4, P4 that kind stores index from high to low, and data show first filial generation reciprocal cross result not Equally, for storage index with maternal related, the high first filial generation of female parent storage index is corresponding also higher.806 serial tomato first filial generations are green Fruit stores index between two parents, and 808 serial tomatoes show lower than corresponding parent.

When red fruit stores index measurement, F2 and P3 do not measure (field does not have red fruit), other 6 kinds pass through card side point Analysis show that there are extremely significant difference, compared with green fruit, the storage index of red fruit is all smaller, after storage 50 days, up to 42.27, Minimum only 3.24.Storing index from high to low is respectively P1, F1, P2, F4, F3, P4, and P4 red fruit is than other during the experiment Kind tomato shows obvious not storage tolerance.

It is examined by T and shows the storage index of green fruit and red fruit there are significant differences, by data it can also be seen that green fruit stores The higher red fruit storage index of index also can be higher.

By test process, it can also be seen that, after storage 20 days, different quality tomato annesl situations are there are significant difference, wherein P4 All turn red, P3 is unable to annesl, other parts annesl.Annesl situation from it is good to difference be respectively P4, F1, P1, F4, F3, F2, F3, P2,P3.By the measurement to storage index it can find that storage index and fruit annesl have certain relationship, storage index is big, Its fruit annesl situation is accordingly poor.

(2) hardness of fruit

When sampling, P4 material is without red fruit and annesl fruit.As can be seen from Table 15, in the red ripe phase, the hardness of fruit of various tomatoes There are extremely significant differences, and then are found by Multiple range test, significant difference between P2, F1, F2, significant difference between F1, F2, Significant difference between significant difference F3, F4 between P3, F3, F4.This illustrates that red ripe phase fruit parental generation and first filial generation, first filial generation are positive and negative The hardness of fruit between friendship is there are significant difference, and the parental generation hardness of fruit is big, and the first filial generation hardness of fruit is also big, and maternal Hereditary transmission capacity it is stronger.

In veraison, for the hardness of fruit of various tomatoes there are extremely significant difference, Multiple range test discovery is poor between P1, F1, F4 Different not significant, difference is not significant between F2, F3, and there are significant differences for first three and latter two, but difference is not shown between F3, F4 It writes.This illustrates that difference is not present in the hardness of fruit between veraison parental generation and first filial generation, and 808 serial first filial generation reciprocal crosss exist significant Difference, and difference is not present in 806 serial reciprocal crosss.

In 7 measured kinds, there is significant difference (P2, P3 removed in red ripe phase and veraison tamato fruit hardness Outside), the veraison hardness of fruit is significantly higher than red ripe phase fruit, under this shows that tamato fruit hardness is significant during Ripening and Softening Drop.

11 different ripening stages tamato fruit nonhomogeneous hardness of table

Table11Difference of Hardness between Different Ripening Stages.

Data are average value ± standard error

Date are presented as mean±SE.

(2) soluble solid

Soluble solid content compares in the different tomatoes of table 12

Table 12Comparison of soluble solid content in Different Tomatoes.

Data are average value ± standard error, show significant difference in the different subscript of same row.

Date are presented as mean±SE.The different superscripts in the same row indicate significant differences.

As can be seen from Table 12, all there is significant difference in the soluble solid of different tomatoes, red ripe in red ripe phase and veraison When the phase, Multiple range test finds F1 and F2 significant difference, and significant difference between F3 and F4, this shows exist between red ripe phase reciprocal cross Significant difference.

During Ripening and Softening, downward trend is presented in soluble solid, and wherein F4 decline is most fast.

(4) PG enzyme

All there is significant difference (being shown in Table 13) between various tomatoes in phase same time during Ripening and Softening.When green ripe stage, PG Enzyme activities trend is F2, F4, P2, P1, F1, P3, F3 from high to low, wherein preceding 3 inter-species, rear 4 inter-species are without significant difference； When veraison, PG Enzyme activities trend is F1, F4, F2, P1, F3 from high to low, wherein after 4 kinds without significant difference；The pink phase When, PG Enzyme activities trend is F4, F1, F2, F3, P1 from high to low, wherein without significant difference between F1 and F2, F3 and P1； When the red ripe phase, PG Enzyme activities trend is P4, F2, F4, F1, F3, P1 from high to low.

During Ripening and Softening, PG enzymatic activity is gradually increased, significant in different ripening stages variation, through Multiple range test It was found that green ripe stage and veraison have no significant difference, other when during there are significant differences, this shows from green ripe stage to annesl Phase, tomato PG Enzyme activities are little, weaker, and activity very fast rising when from veraison to the pink phase, on the red ripe phase continues It rises, reaches maximum.

By Multiple range test as can be seen that each period in maturation, F1 and F2, equal difference is aobvious between F3 and F4 It writes, this illustrates that there are significant differences between reciprocal cross.

The different tomato PG enzyme activity variances in maturation of table 13 compare

Table 13 Comparison of PG Activities Among Different Tomatoes During Ripening Stages.

2, fruit quality is analyzed

(1) total Soluble Sugar

The different tomato soluble sugar content comparison in difference in maturation of table 14

Table 14 Comparison of Soluble Sugar Content Among Different Tomatoes During RipeningStages

As shown in table 14, during Ripening and Softening, soluble sugar content variation is obvious, but each interracial variation tendency It is not consistent.P4, F4, P1, P2 are high in green ripe stage content, then begin to decline, P4 slightly rises in the pink phase, and the red ripe phase subtracts again Small, F4 is continued to decline, and P1 reaches minimum in the pink phase, then bottom out, and can reach the content of green ripe stage.And F3, P3, F2, F1 are relatively low in green ripe stage content, then begin to ramp up.F3 rising is most fast, reaches maximum in the pink phase, then declines. F2, F1 variation are more gentle, and F2 declines in veraison, and persistently rise in pink phase Shi Shangsheng, F1, after the pink phase slightly under Drop.Tomato soluble sugar content concentrates between 2-20mg/g, maximum value 19.9413mg/g.As can be seen from Table 13, different tomatoes In green ripe stage, pink phase, red ripe phase soluble sugar content significant difference, and in veraison various tomatoes soluble sugar content It is closer to, has no significant difference.The various tomato soluble sugar contents of green ripe stage be from high to low P4, F4, P2, P1, F3, P3, F2, F1, wherein without significant difference between P4 and F4, P2 and P1, F3 and P3, P3 and F2；Pink phase various tomato soluble sugar contents from It is high to Low be F3, P4, F1, F4, P1, F2, wherein each kind two-by-two between there is significant difference；Red various tomatoes of ripe phase are soluble Sugared content is P1, P4, F3, F1, F2, F4 from high to low, wherein without significant difference between P4 and F3, F1 and F2.

(2) vitamin C

As shown in Table 15, during Ripening and Softening, change of vitamin C is obvious, but the variation tendency of various tomatoes It is essentially identical, only slightly have difference in the time that peak value occurs.In addition to P1 Vitamin C content peak appear in the pink phase with Outside, other materials Vitamin C content peak appears in veraison, as the mature Vitamin C content of tamato fruit declines.Respectively Kind tomato Vitamin C content concentrates between 160-300 μ g/g, and highest content can reach 300.0500 μ g/g, and minimum value is 144.4600μg/g.Secondly the wherein highest F4 for veraison of Vitamin C content is the P1 of pink phase, is then veraison P4.Reciprocal cross material is shown in equal significant difference, due to there is a parent material for not annesl material, does not measure its vitamin C Content, thus cannot relatively more corresponding hereditary transmission capacity.

The different tomato Vitamin C content comparison in difference in maturation of table 15

Table 15Comparison of Vc Content Among Different Tomatoes During Ripening Stages.

(3) lycopene

The different tomato lycopene content comparison in difference in maturation of table 16

Table16 Comparison of lycopene Content Among Different Tomatoes During Ripening Stages.

As shown in table 16, various tomatoes are extremely low in green ripe stage lycopene content, close to 0, with tamato fruit at Ripe, lycopene content is gradually increasing, and reaches maximum in the red ripe phase, lycopene content concentrate on 0.005-0.025mg/g it Between, maximum value can reach 0.04256010mg/g, and wherein the variation of P4 lycopene content is most obvious.As can be seen from Table 20, in difference Maturity period, there is significant difference in lycopene content between each kind tomato.The various tomato lycopene contents of green ripe stage from High to Low is F1, F4, F2, P4, P2, F3, P1, wherein F1 and F2, P4 and P2, is not present significant difference between P2 and P1, F3 and F4 significant difference.Veraison various tomato lycopene contents are F2, P1, F3, P4, F1, F4 from high to low, poor between parent material It is different significant, and Reciprocal crosses are significant.Pink phase various tomato lycopene contents be from high to low P4, P1, F3, F1, F4, F2, wherein each kind two-by-two between there is significant difference.Red various tomato lycopene contents of ripe phase be from high to low P4, F1, Significant difference, significant difference between other materials is wherein not present in P1, F4, F3, F2 between F1 and P1.

By experiment:

From the point of view of storing index investigation, first filial generation reciprocal cross result is different, and storage index is maternal with maternal related It is corresponding also higher to store the high first filial generation of index；The higher red fruit storage index of green fruit storage index also can be higher；Storage refers to Several have certain relationship with fruit annesl, and storage index is big, and fruit annesl situation is accordingly poor.

From the point of view of hardness of fruit measured value, there is significant difference, veraison in red ripe phase and veraison tamato fruit hardness The hardness of fruit is significantly higher than red ripe phase fruit, this shows that tamato fruit hardness is remarkably decreased during Ripening and Softening；The red ripe phase The hardness of fruit between fruit parental generation and first filial generation, first filial generation reciprocal cross is there are significant difference, and the parental generation hardness of fruit is big, Its first filial generation hardness of fruit is also big, and maternal hereditary transmission capacity is stronger.This is as the result of storage index reflection.

From the point of view of the result that soluble solid measures: there are significant differences between red ripe phase reciprocal cross, and mature soft During change, downward trend is presented in soluble solid.

From the point of view of the result of PG enzymatic determination: during Ripening and Softening, PG enzymatic activity is gradually increased, green ripe stage and veraison Have no significant difference, other when during there are significant differences, this shows from green ripe stage to veraison, and tomato PG Enzyme activities are not Greatly, the very fast rising of activity, red ripe phase continue to rise when and from veraison to the pink phase, reach maximum；It is deposited between reciprocal cross simultaneously In significant difference.

From the point of view of the result that total Soluble Sugar measures: there are significant differences between red ripe phase reciprocal cross, and in Ripening and Softening In the process, downward trend is presented in soluble solid.

From the point of view of the result that correlation of attributes factor measures: due to different materials maturity period difference, thus in storeroom correlation The more difficult analysis of property, only from performance optimal from the point of view of the result measured between individual be P4, be secondly P1, be then F4, F1, F3, F2。

3, comprehensive analysis

(1) keeping quality

It is very popular to the cultivation of keeping quality tomato and research in recent years, studies have found that during Ripening and Softening, The decline of tamato fruit soluble solid, PG enzyme content rise, and external manifestation becomes smaller for the hardness of fruit, and softening is rotted, and PG Enzyme plays very important effect.Simultaneously found in the research process to keeping quality tomato, storage property mutant with compare For group compared to having very high storage index, Storage is much better.

In Tomato Ripening softening process, the hardness of fruit, soluble solid content are gradually reduced, PG enzyme content gradually on It rises.Further analysis is found in the different maturity period hardnesses of fruit, soluble solid content, there are significance differences for PG enzyme content It is different.This illustrates that the Ripening and Softening of PG enzymatic activity and fruit has substantial connection, and PG enzymatic activity is bigger, the hardness of fruit and soluble solid Object content is lower.But it was found that the hardness of fruit and the variation tendency of soluble solid are similar, i.e. fruits between tomato not of the same race Its soluble solid content of the big kind of real hardness is higher, this illustrates that soluble solid has certain shadow to the hardness of fruit It rings.

As for storage index, green fruit is significantly higher than red fruit, and there is also significant differences between different tomato varieties.Storing index is Fruit keeping quality most intuitively shows, and storage index height then shows fruit storage tolerance, and the shelf time is long.In green fruit between each kind Or store index when red fruit and there is significant difference, and their height variation tendency is consistent, this illustrates these resistance to storages kind There are significant differences for the keeping quality of eggplant.This research also found that there are certain passes with PG enzyme for the storage index of various tomatoes System stores that index is big, and PG enzyme content is opposite just lower in the identical maturity period.

By each index of keeping quality of the serial tomato of analysis 808 and 806, it is found that two serial certain qualities have phase As Storage, difference is all not present at the hardness of fruit, soluble solid, the aspect of PG enzyme three between F1 and F4, F2 and F3, This illustrates that significant difference is not present between corresponding first filial generation in terms of storage.Research finds the storage feature of parental generation and first filial generation There is certain connection, the storage index of first filial generation is partial to female parent material between parental generation.

The tested green fruit Storage of kind tomato is good, and healthy fruit and storage index are still very high after P2, P3 are stored 50 days, but they Fruit annesl it is in bad order, be unable to annesl or annesl be uneven, if can solve this problem in the tomato breeding in future, will Greatly improve the storage quality of tomato.

(2) nutritional quality

The nutritive value quality of tamato fruit mainly includes total Soluble Sugar, vitamin C, lycopene etc..Especially tie up Raw element C and lycopene, as people increasingly pay close attention to healthy in recent years, they are two also increasingly by many researchers' Concern.Studies have pointed out that the appearance of keeping quality tomato, mouthfeel be not as good as Lycopersicon esculentum, therefore improve the battalion of keeping quality tomato It is most important to support quality.

Total Soluble Sugar, vitamin C, lycopene all change significantly during Ripening and Softening, wherein solvable Property total reducing sugar, vitamin C variation tendency in different cultivars tomato are inconsistent, and lycopene then shows to be gradually increasing trend, because This lycopene has more researching value, other two kinds of need carry out further experiment research.Pass through the ratio to different tomato varieties Compared with showing that these three qualities all have significant difference in each maturity period, this illustrates different cultivars tomato in terms of nutritive value It is what there were significant differences.From analysis of experimental results, the trend of red ripe three kinds of qualities of phase is substantially uniform, thus infer nutritive value compared with That high is P1 and P4.

In short, finding PG enzyme and solubility between different tomato varieties in conjunction with the comparison of storage quality and nutritive value quality Total sugar content relationship is more close, and general relationship is that its Soluble adhesion molecule of PG enzymatic activity height kind is lower.

The above disclosure is only the preferred embodiments of the present invention, cannot limit the right model of the present invention with this certainly It encloses, therefore equivalent changes made in accordance with the claims of the present invention, is still within the scope of the present invention.

Claims

1. a kind of storage tolerance tomato selection, which comprises the following steps:

(1) it introduces that Comprehensive Traits are excellent and material containing slow ripe gene mutation body by seed collection, branch acquisition, carries out continuous The tomato resources of storage tolerance are isolated in selfing, screening；

(2) hybridization apolegamy is carried out for screening isolated material, with relatively high for female parent, storage index of storing index in combination It is relatively low for male parent, hybridized, obtain storage tolerance tomato；

2. storage tolerance tomato selection according to claim 1, it is characterised in that: in step (2), to the son after hybridization A generation is cultivated, and random sampling is carried out during cultivation, investigates plant biology shape, Fruit Characteristics, and in harvesting latter stage system Commodity fruit yield is counted, following kind is screened out: 1. single fruit weight is less than to the kind of 180g；2. defective, dehiscent fruit or sky by fruit face The ratio that the quantity of hole fruit accounts for the total number of fruits is greater than 10% kind.

3. storage tolerance tomato selection according to claim 1, it is characterised in that: in step (1), screen and isolate Yellow fruit resource is as follows:

Lima company, 720-4-2 strain material source France forms, major traits are as follows: indeterminate growth through continuous 8 generation selfed breeding Type, growth potential are strong；Dark green leaf, fruit setting power are strong；Calyx is big calyx, calyx gauffer；Fruit circle, shoulder of having no result, Chinese olive is white, Mellow fruit is yellow fruit, and fruit is hard, stores without putrefaction in six months at room temperature；3~4 ventricle of fruit, pulp are averaged thickness 10mm, single fruit weight 200~240 grams；

Lima company, 720-6-1-11 strain material source France forms, major traits through continuous 11 generation selfed breeding are as follows: unlimited Growth type, plant type is sparse, dark green leaf, and fruit setting power is strong；Fruit circle, shoulder of having no result, Chinese olive is white, and mellow fruit is yellow fruit, fruit Firmly；3~4 ventricle of fruit, pulp are averaged thickness 8.5mm, and 170 grams of single fruit weight or so；

Lima company, 720-9-1-6 strain material source France forms, major traits are as follows: anti-yellowing through continuous 11 generation selfed breeding Change leaf curl viral disease；Indeterminate growth type, growth potential is medium, dark green leaf, and fruit circle, shoulder of having no result, Chinese olive is white, and mellow fruit is Yellow fruit, fruit are hard；3~4 ventricle of fruit, pulp are averaged thickness 9.5mm, and 160 grams of single fruit weight or so；

Lima company, 716-2-2-15 strain material source France forms, major traits through continuous 8 generation selfed breeding are as follows: unlimited Growth type, growth potential is strong, dark green leaf, and fruit setting power is strong；Calyx is big calyx, spray acropetal leaf；Fruit justifies slightly flat, fruit face Smooth, fruit type is good, and shoulder of having no result, Chinese olive is white, and mellow fruit is yellow fruit, and single fruit is big, and fruit is hard；2~4 ventricle of fruit, the average thickness of pulp 10mm, 200~220 grams of single fruit weight；

712 Lima companies, short Huang -1-6 strain material source France, form, major traits are as follows: limited through continuous 8 generation selfed breeding Growth type, growth potential are strong；Blade is big, potato blade profile, light green color, and flowering fruit is good under low temperature；Calyx is big calyx, fruit Circle, Chinese olive is white, shoulder of having no result, mature fruits yellow；Fruit sepal is big, light green color, the oversensitive room of fruit, firmly, average fruit kind 220 Gram；

Lima company, 78-3-1-11 strain material source France forms through continuous 8 generation selfed breeding；Major traits are as follows: unlimited raw Long type, growth potential is strong, dark green leaf, and fruit setting power is strong；Fruit is oblate, and fruit type is good, and mellow fruit is yellow fruit, and fruit is hard；Fruit 3 ~5 ventricles, pulp are averaged thickness 6.5mm, and 200~210 grams of single fruit weight；

Lima company, 711-2-35-19 strain material source France forms through continuous 11 generation selfed breeding；Major traits are as follows: anti- Tomato yellow leaf curl China viral disease；Indeterminate growth type, plant type is sparse, and cold resistance is good, dark green leaf, and calyx is more open and more flat；Fruit is round Slightly flat, light fruit shoulder, Chinese olive is green, and mature fruits large red, uniform coloring, pulp is red, and pulp is averaged thickness 6.5mm, fruit 3~4 Ventricle, 150 grams of mean fruit weight；

3-22-2-1 strain material source Israel Hai Zela is formed by FA189 through continuous 13 generation selfed breeding；Major traits Are as follows: indeterminate growth type, growth is medium, and cold resistance is good, and fruit setting power is strong；Fruit circle is slightly flat, and light fruit shoulder, Chinese olive is green, mellow fruit Fruit large red, uniform coloring；Fruit sepal is elongated, and bottle green, carpopodium is short, and inflorescence is unfolded；Every Honoka florescence neat and consistent, often 3~4 flowers of Honoka sequence；4~5 ventricle of fruit, 220 grams of mean fruit weight.

4. storage tolerance tomato selection according to claim 1, it is characterised in that: in step (2), using there is native cultivation Training, experimental field applies decomposed dung 800kg, calcium magnesium phosphate 50kg per acre, and ternary compound fertilizer is base manure, entire breeding time top dressing 4 times, Accumulative mu imposes urea 60kg, ternary compound fertilizer 50kg, and potassium chloride 60kg is experimental field covered using fly net, right during test Bemisia tabaci carries out repeatedly spray prevention and treatment.

5. storage tolerance tomato selection according to claim 1, it is characterised in that: in step (2), planted using without soil Training, in sowing the latter moon seedling separation, another is colonized in cultivation bed after the moon, cultivation bed is made of cystosepiment, and one is spread in slot Layer silver gray plastic foil simultaneously open aperture by 0.8m spacing, after fill out using the sawdust that ferments as the mixed-matrix of primary raw material, cultivated Nutrient fluid drip irrigation is used in journey, test uses RANDOMIZED BLOCK DESIGN, 2 repetitions, and every cell plants 92 plants of tomato, spacing in the rows 0.20m, Using Netted melon, the oblique hanging tendril of lanyard, odd number strain unifies lanyard in cultivation bed upper left side, and even number strain unifies lanyard in cultivation bed Upper right side promotes fruit setting using drop-proof agent spraying decoration, and test greenhouse surrounding uses 30 mesh fly net insect prevention, experimental field in by between certain Away from Yellow sticky trap is hung, 3 spray prevention and treatments are carried out during test altogether to Bemisia tabaci.

6. storage tolerance tomato selection according to claim 1, it is characterised in that: in step (3), storage test process As follows: in the Yu Caishou later period, in experimental plot, the fruit of annesl, no disease and pests harm and mechanical damage is completed in 90% or more fruit face of picking, Each fruit picking 100 of each kind, arrange carry out room temperature storage test, storage period room temperature be 20 DEG C ± 5 DEG C, humidity be 80% ± 5%, the fruit for losing commodity is recorded and rejected daily, until each kind healthy fruit is reduced to 50% or so end storage test, and Count storage period number of days and bad fruit type and ratio.

7. storage tolerance tomato selection according to claim 1, it is characterised in that: in step (3), storage test process It is as follows: Yu Caishou mid-term, taken out of each cell respectively disease-free, neat and consistent tamato fruit several, be put into plastic crate, It impregnates to take out after five minutes through 2.5% sodium hypochlorite and dry, be placed on 25 DEG C under room temperature, primary every investigation in 10 days, removal is lost The fruit for going commodity value calculates green fruit and red fruit healthy fruit and storage index.