CN109837259A - From the thermophilic esterase crystal three-dimensional structure and expression and purification method of marine bacteria - Google Patents

From the thermophilic esterase crystal three-dimensional structure and expression and purification method of marine bacteria Download PDF

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CN109837259A
CN109837259A CN201910134759.1A CN201910134759A CN109837259A CN 109837259 A CN109837259 A CN 109837259A CN 201910134759 A CN201910134759 A CN 201910134759A CN 109837259 A CN109837259 A CN 109837259A
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atom
leu
amino acid
ala
esterase
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CN109837259B (en
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李继喜
胡小健
李正阳
吴敌
季朝能
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Fudan University
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Fudan University
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Abstract

The invention belongs to field of biotechnology, specially derive from the thermophilic esterase crystal three-dimensional structure and its expression and purification method of marine bacteria.The thermophilic esterase AlinE4 contains 190 amino acid, and molecular weight is about 20.5 kDa, and isoelectric point is about 6.8；In expression bacterial strainEscherichia coli There is highly soluble expression in BL21, and show good heat-resisting, alkaline-resisting, salt tolerant, resistance to heavy metal property；Esterase AlinE4 obtains its three-dimensional structure by the method for X-ray diffraction, and resolution ratio reaches 1.18.The structure is by 5 β-pleated sheets, 9 α spiral shells and 2310Spiral composition；Wherein, the 13rd serine, the 162nd aspartic acid and the 165th hyte propylhomoserin constitute the enzyme activity center；13rd serine, 50 glycine and 81 asparagines form oxonium ion channel.The three-dimensional structure of esterase AlinE4 can screen in chiral precursor, be with a wide range of applications in terms of substrate transformation and raising enzyme activity in industrial enzyme preparation.

Description

From the thermophilic esterase crystal three-dimensional structure and expression and purification method of marine bacteria
Technical field
The invention belongs to field of biotechnology, and in particular to one kind from marine bacteria soap albumin enzyme crystal structure and its Expression and purification method.
Background technique
Esterase (esterase EC 3.1.1.1) is a kind of water that lipid can be decomposed into acid and alcohol by chemical reaction Solve enzyme.Usually there is the catalysis triangle being made of three serine, histidine and aspartic acid amino acid residues in esterase For its activated centre；Wherein, serine residue frequently appears in such a five conservative peptide sequences of GXSXG.Most of ester Confactor participation is not needed when enzymic catalytic reaction, and has popularity and certain stereochemical structure special reaction substrate Property, these characteristics pay close attention to it increasingly by people as a kind of biocatalyst.Nowadays, esterase is as a kind of environment friend Good, economic and clean catalyst plays more next in fields such as food, papermaking, fine chemistry synthesis and medical diagnosis More important role.The esterase industrially applied is mainly derived from different organisms, particularly from fungi and bacterium, This is mainly due to microbe-derived esterases to have yield height, stable reaction, by-product small toxicity and molecular biology manipulations The advantages that simple.
The esterase of marine source usually has an advantageous property relevant to marine environment, for example, temperature stability, salt tolerance, Alkali resistance, low temperature resistant and excellent chiral selectivity etc., therefore, filter out the ester to show unique characteristics from marine microorganism Enzyme just screens at chiral precursor and develops an important directions of infant industry enzyme preparation.The present invention utilizes the side crystallized Method has obtained the albumin crystal of esterase AlinE4 and has parsed its three-dimensional structure.AlinE4 crystal structure can be in chiral precursor It is with a wide range of applications in terms of substrate transformation and raising enzyme activity in screening, industrial enzyme preparation.
Summary of the invention
The purpose of the present invention is to provide a kind of expression and purification methods of albumen esterase from marine bacteria, its crystal Three-dimensional structure.
The present invention includes providing to from marine bacteriaAltererythrobacter indicus DSM 18604T's Esterase AlinE4 carries out genetic engineering transformation to obtain the high efficient expression in Escherichia coli, purifying and the method for crystallization, and The esterase AlinE4 the 1st is to the three-dimensional crystalline structure of the 190th amino acids and its in the application of industrial aspect.
The present invention will derive from marine bacteria firstAltererythrobacter indicus DSM 18604TEsterase AlinE4 has carried out genetic engineering transformation, its full-length gene is cloned on the carrier of pSMT3.Esterase AlinE4 amino acid sequence As shown in SEQ.ID.NO1, nucleic acid sequence such as SEQ.ID, shown in NO2.
The present invention preferentially select using Escherichia coli procaryotic cell expression system (but be not excluded for other expression systems, Such as expressed in other bacteriums or other eukaryotic cells), to albumen described above in a manner of fusion protein It is expressed, has preferentially selected His label to obtain solubility expression and (but be not excluded for other labels, such as MBP or GST label Also there is solubility expression).
The present invention also provides a kind of expression and purified source Yu Haiyang bacteriumsAltererythrobacter indicus DSM 18604TEsterase AlinE4 method, this method comprises: building fusion wild typealine4The recombination of full-length gene carries Body converts Escherichia coli with the carrier, so that expression has the fusion protein AlinE4 of label, the albumen of above-mentioned acquisition is passed through Specificity the method with the special tag recognition, preferably Ni affinity chromatography (separation principle are as follows: low imidazole concentration buffer into Row loading, high imidazole concentration buffer are eluted) separation destination protein, then obtained by the method for gel permeation chromatography high-purity The albumen of the AlinE4 of degree.
Wherein, the upstream primer for expanding esterase full genome is aline4F(5 '-TCGCGGATCCATGGGCGAATCGCG-3’ (SEQ. ID. NO3),BamHI) and downstream primer is aline4R (5 '-TCCGCTCGAGTCACTTCTTCGCAG-3 ' (SEQ. ID. NO4),XhoI)；Expression plasmid is pSMT3, and method for transformation is CaCl2 conversion method, and conversion bacterial strain is bacillus coli DH 5 alpha, Expression bacterial strain is e. coli bl21 (DE3).
Wherein, the equilibration buffer of the Ni affinity chromatography preferentially selects: 50mM Tris(pH 8.0), 100-500mM NaCl, 5% glycerol, 10mM imidazoles, 2mM β-Me, 0.2mM PMSF；
The Ni affinity chromatography elution buffer preferentially selects: 50mM Tris, pH 8.0,500mM NaCl, 5% glycerol, 250mM Imidazoles, 2mM β-Me；
The buffer of the gel permeation chromatography preferentially selects: 20mM Tris(pH7.5), 100 mM NaCl, 2mM DTT.
The present invention also provides the method for crystallizing esterase AlinE4 obtained above, specific steps are as follows: esterase AlinE4 is dense Being reduced to concentration is 5-30mg/ml, uses crystalline reagents box (the Crystal Screen from companies such as Hampton Research Kit I/II, Index, Salt, PEG/Ion etc.) primary dcreening operation condition as crystal growth, using hanging drop vapor phase grafting into Row crystallization.Temperature is 4-25 degrees Celsius, and preferential selection uses 18 degrees Celsius.
Crystallization condition: being crystallized using hanging drop vapor phase grafting, protein solution and crystalline reagents volume in crystallization drop Than in the range of 3:1 to 1:3, preferred volume ratio 1:1, protein concentration is 5-60mg/ml, crystallization condition in protein solution For 10-50mM cadmium sulfate, 0-100mMHepes(pH 7.5), 0.1-1M sodium acetate.Crystal culture at 4 to 25 degrees Celsius (preferably 18 degrees Celsius) it carries out.
The present invention also provides the crystal of the good esterase AlinE4 protein of diffraction property.
The present invention also provides the three-dimensional structure of esterase AlinE4, which describes the secondary structure group of esterase AlinE4 At peptide chain trend, enterprise schema and three-dimensional molecular construction.Wherein, diffraction is carried out for esterase AlinE4 albumen, obtains esterase The crystal diffraction data of AlinE4 albumen, to derive from bacteriumPseudomonas aeruginosaHomologous GDSL family ester Enzyme TesA(PDB ID:4JGG) it is that model carries out structure elucidation, finally obtain the three-dimensional structure of esterase AlinE4 albumen.
In a particular embodiment, the crystal three-dimensional structure of esterase AlinE4 is provided, the esterase AlinE4 contains 190 A amino acid, molecular weight are about 20.5 kDa, and isoelectric point is about 6.8；Atom in the crystal three-dimensional structure has institute in table 1 At least 40% atomic coordinates of column or any atom three with the main chain carbon skeleton of at least 40% amino acid residue in the coordinate The average root variance for tieing up coordinate is less than or equal to 1.2 structure.
Present invention obtains the crystal three-dimensional structure of preferred esterase AlinE4, resolution ratio 1.2, crystal structure spaces Group is P 42 212.Cell parameter are as follows: a=79.783, b=79.783, c=61.010, α=β=γ=90.000 °.
The crystal structure of esterase AlinE4 of the present invention or its foldable portion, with the original listed in Fig. 1,2,3 The three-dimensional structure that subcoordinate (x, y, z) ± (1.0,1.0,1.0) determines.
In a specific embodiment, the three-dimensional structure of the esterase AlinE4 is in a crystallography asymmetry unit Inside there is a protein molecular.The structure is altogether by 5 β-pleated sheets, 9 α spirals and 2310Spiral composition.
Wherein, 5 β-pleated sheets are located at center, and 9 α are spiraled about around β-pleated sheet.Wherein, β rolls over Folded 1, i.e. the amino acid section containing Arg5-Gly11；α spiral 1, i.e. the amino acid section containing Ser13-Ala16；α spiral 2, that is, contain The amino acid section of Tyr26-His37；β-pleated sheet 2, i.e. the amino acid section containing Ala41-Gly47；α spiral 3 contains Thr53- The amino acid section of Asp65；β-pleated sheet 3, i.e. the amino acid section containing Leu73-Glu77；α spiral 4, i.e., containing Gly80-Leu84's Amino acid section；α spiral 5, i.e. the amino acid section containing Pro89-Arg105；β-pleated sheet 4, i.e., containing the amino of Ile110-Met113 Sour section；310Spiral 1, i.e. the amino acid section containing Pro119-Leu121；α spiral 6, i.e., containing the amino acid of Ala123-Asp130 Section；α spiral 7, i.e. the amino acid section containing Ile132-Lys139；β-pleated sheet 5, i.e. the amino acid area containing Lys143-Val145 Section；α spiral 8, i.e. the amino acid section containing Phe148-Val152；310Spiral 3, i.e. the amino acid area containing Pro156-Leu158 Section；α spiral 9, i.e. the amino acid section containing Ala168-Ala185.
The present invention also provides the mechanism of action that esterase AlinE4 plays esterase hydrolyzed function.The esterase AlinE4 crystal In structure, the 13rd serine (Ser13), the 162nd aspartic acid (Asp162) and the 165th hyte propylhomoserin (His165) are constituted It is catalyzed three disjunctors composition activated centre.Activated centre is occupied by a cadmium ion, as shown in Figure 3.
The crystal structure of the esterase AlinE4, the 13rd serine (Ser13), the 50th glycine (Gly50) and the 81 asparagines (Asn81) constitute oxonium ion channel.
The present invention also provides the three-dimensional structures of esterase AlinE4 in chiral precursor screening, substrate in industrial enzyme preparation Application in terms of transformation and raising enzyme activity.
Detailed description of the invention
Fig. 1 is esterase AlinE4 monolithic three dimensional structure cartoon schematic diagram, α spiral and 310Spiral blue markings, β-pleated sheet make With yellow flag, random coil is indicated with grey.
Fig. 2 is esterase AlinE4 surface charge distribution and active site schematic diagram, cloud density level=1.0.
Fig. 3 is activated centre cadmium ion and catalytic triads interaction schematic diagram, cloud density level=1.0.
Fig. 4 is the protein purification and crystal the selection result of esterase AlinE4.Wherein, on (A): AlinE4 molecular sieve result is shown It is intended to, the pillar used is 75 16/600(GE of Superdex, the U.S.)；Under: the SDS- of AlinE4 molecular sieve elution samples PAGE testing result, left lane are albumen Marker.(B) AlinE4 crystal picture.
Specific embodiment
The contents of the present invention are further illustrated using specific embodiment below.
The following content is a further detailed description of the present invention in conjunction with specific preferred embodiments, and it cannot be said that Specific implementation of the invention is only limited to these instructions.For those of ordinary skill in the art to which the present invention belongs, exist Under the premise of not departing from present inventive concept, a number of simple deductions or replacements can also be made, all shall be regarded as belonging to of the invention Protection scope.
The expression and purification of 1. esterase AlinE4 of embodiment
From marine bacteriaAltererythrobacter indicus DSM 18604TEsterase AlinE4, amino acid Sequence is as shown in SEQ.ID.NO1, nucleic acid sequence such as SEQ.ID, shown in NO2.
By molecule clone technology by esterasealine4Full-length gene be cloned on the carrier of pSMT3, for expressing ammonia Recombinant vector is transformed into e. coli bl21 (DE3) by the fusion protein of 6 His of base terminal fusion, 37 DEG C of shaken cultivations The IPTG that final concentration of 0.5 mM is added when reaching 0.8 to OD600 carries out inducing expression, expression condition are as follows: 16 DEG C of shaken cultivations 20h or 20 DEG C of shaken cultivation 16h or 25 DEG C of shaken cultivation 8h.
The recombinant expression carrier built is transformed into the solubility that esterase AlinE4 is carried out in e. coli bl21 (DE3) Expression.It will be selection pressure with 50 μ g/ml kanamycins and 34 μ g/ml chloramphenicol, switching recombinant strains are in containing upper In the LB liquid medium for stating selection pressure, 37 DEG C of shaken cultivations to OD600 are added final concentration of 0.5 mM's when reaching 0.8 IPTG carries out inducing expression, preferred expression condition are as follows: 16 DEG C of shaken cultivation 20h.It is added when reaching 0.8 to OD600 final concentration of The IPTG of 0.5 mM carries out inducing expression.16 DEG C, 200rpm shaken cultivation, by the way that bacterium is collected by centrifugation after 20 hours, for pure Change.
By the expression bacterium being collected by centrifugation with appropriate amount of buffer solution (50mM Tris (pH 8.0), 500mM NaCl, 5% glycerol, 10mM imidazoles, 2mM β-Me, 0.2mM PMSF) bacterium is hanged, use low-temperature ultrahigh-pressure cell crushing instrument (Guangzhou cumulative biotechnology Co., Ltd) cracking thallus, with 18000rpm high speed centrifugation 1h removal precipitating and other particulate contaminations.By centrifuged supernatant After in conjunction with Ni-NTA affinity media, with 50mM Tris (pH 8.0), 500mM NaCl, 5% glycerol, 50mM imidazoles, 2mM β- The buffer scouring media of Me removes foreign protein.50mM Tris (pH 8.0) finally is used, 500mM NaCl, 5% glycerol, 250mM The eluent of imidazoles, 2mM β-Me elutes destination protein from affinity media, will be washed with the concentration tube that 10 KDa are retained De- liquid concentration.The method that protein solution after concentration further uses gel permeation chromatography (superdex75,16/600) is purified, The buffer used is 20mM Tris (pH7.5), 100 mM NaCl, 2mM DTT.The destination protein eluted is carried out dense Being reduced to concentration is 60mg/ml, is saved in -80 DEG C, and crystallization experiment is used for.
The crystallization of 2. esterase AlinE4 of embodiment
It is about 60mg/ml that the good esterase AlinE4 of method expression and purification as above, which is concentrated into concentration, (is come from using crystalline reagents box Crystal Screen Kit I/II, Index, Salt, PEG/Ion of the companies such as Hampton Research etc.) conduct The primary dcreening operation condition of crystal growth is crystallized using hanging drop air plllutant method.The present invention is in multiple and different crystalline reagents conditions Under obtain primary crystalline.Pass through optimizing and revising for later period, preferably 50mM cadmium sulfate, 100mM Hepes(pH 7.5), 1M second The buffer of sour sodium is collected into the X ray diffracting data that a set of resolution ratio is 1.18 as crystal growth condition.
The receipt of 3. esterase AlinE4 of embodiment is collected and structure elucidation
After the present invention handles the crystal of preparation using 25% glycerol as antifreezing agent, freeze in liquid nitrogen.Crystal X-Ray Diffraction Data collection shanghai light source (SSRF) biological macromolecule crystal light beam line station (five lines, six station BL17U1) carry out.At data Reason uses HKL2000, and structure elucidation is to derive from bacteriumPseudomonas aeruginosaHomologous GDSL family esterase TesA(PDB ID:4JGG) it is model, using molecular replacement technique, using Refmac program and it is aided with Coot software and does and further repair Just, final parsing obtains the crystal structure of esterase AlinE4 albumen.Those skilled in the art are it is found that in the wherein crystalline substance Atom in body three-dimensional structure has in the crystal three-dimensional structure of at least 40% atomic coordinates or 3D albumen listed in table 1 The atomic structure coordinate of the main chain carbon skeleton of at least 40% amino acid and the average root variance of the coordinate in table 1 are less than or equal to 1.2 Atomic coordinates, can be considered that there is identical structure with 3D albumen.
Specifically, the crystal three-dimensional structure of the esterase AlinE4, in a crystallography asymmetry unit there are two Asymmetric molecult.By 5 β-pleated sheets, 9 α spirals and 2310Spiral composition, wherein 5 β-pleated sheets are located at center, institute 9 α stated are spiraled about around β-pleated sheet.Wherein, β-pleated sheet 1, i.e. the amino acid section containing Arg5-Gly11；α spiral 1, That is the amino acid section containing Ser13-Ala16；α spiral 2, i.e. the amino acid section containing Tyr26-His37；β-pleated sheet 2, that is, contain The amino acid section of Ala41-Gly47；α spiral 3, i.e. the amino acid section containing Thr53-Asp65；β-pleated sheet 3 contains Leu73- The amino acid section of Glu77；α spiral 4, i.e. the amino acid section containing Gly80-Leu84；α spiral 5 contains Pro89-Arg105 Amino acid section；β-pleated sheet 4, i.e. the amino acid section containing Ile110-Met113；310Spiral 1, i.e., containing Pro119-Leu121's Amino acid section；α spiral 6, i.e. the amino acid section containing Ala123-Asp130；α spiral 7, i.e., containing the ammonia of Ile132-Lys139 Base acid section；β-pleated sheet 5, i.e. the amino acid section containing Lys143-Val145；α spiral 8, i.e., containing the amino of Phe148-Val152 Sour section；310Spiral 3, i.e. the amino acid section containing Pro156-Leu158；α spiral 9, i.e., containing the amino acid of Ala168-Ala185 Section.
The atomic coordinates group of albumen esterase AlinE4 crystal is following (referring to table 1):
Crystal space group: P 42 212 ；
Cell parameter are as follows:: a=79.783, b=79.783, c=61.010, α=β=γ=90.000 °；
I/ σ I:20.66；
Rwork/Rfree is respectively as follows: 0.178 and 0.189；
Resolving range (): 48.46-1.18；
Data integrity (%): 99.03.
Table 1
1 N SER A of atom, 0-25.136 6.782 8.343 1.00 13.15 N
2 CA SER A of atom, 0-24.109 5.938 8.933 1.00 14.44 C
3 C SER A of atom, 0-24.369 5.715 10.411 1.00 13.02 C
4 O SER A of atom, 0-25.061 6.500 11.043 1.00 12.87 O
5 CB SER A of atom, 0-22.745 6.600 8.795 1.00 16.23 C
6 OG SER A of atom, 0-22.450 6.888 7.441 1.00 16.45 O
7 N AMET A of atom, 1-23.828 4.622 10.932 0.60 14.33 N
8 CA AMET A of atom, 1-23.950 4.306 12.339 0.60 14.93 C
9 C AMET A of atom, 1-22.860 5.014 13.127 0.60 14.95 C
10 O AMET A of atom, 1-21.779 5.316 12.607 0.60 14.57 O
11 CB AMET A of atom, 1-23.780 2.802 12.541 0.60 17.63 C
12 CG AMET A of atom, 1-24.842 1.983 11.858 0.60 18.90 C
13 SD AMET A of atom, 1-26.398 2.188 12.725 0.60 22.10 S
14 CE AMET A of atom, 1-26.119 1.125 14.136 0.60 23.17 C
15 N BMET A of atom, 1-23.846 4.624 10.963 0.40 14.40 N
16 CA BMET A of atom, 1-24.059 4.313 12.367 0.40 15.02 C
17 C BMET A of atom, 1-22.876 4.793 13.196 0.40 15.27 C
18 O BMET A of atom, 1-21.725 4.729 12.753 0.40 17.88 O
19 CB BMET A of atom, 1-24.236 2.807 12.569 0.40 18.54 C
20 CG BMET A of atom, 1-25.534 2.246 12.004 0.40 18.04 C
21 SD BMET A of atom, 1-25.777 0.516 12.444 0.40 25.65 S
22 CE BMET A of atom, 1-25.532 0.569 14.216 0.40 23.95 C
23 N GLY A of atom, 2-23.165 5.293 14.385 1.00 12.68 N
24 CA GLY A of atom, 2-22.127 5.508 15.366 1.00 14.43 C
25 C GLY A of atom, 2-21.852 6.972 15.653 1.00 12.05 C
26 O GLY A of atom, 2-22.087 7.864 14.836 1.00 13.21 O
27 N GLU A of atom, 3-21.270 7.186 16.828 1.00 12.79 N
28 CA GLU A of atom, 3-20.766 8.488 17.231 1.00 11.17 C
29 C GLU A of atom, 3-19.627 8.920 16.314 1.00 10.90 C
30 O GLU A of atom, 3-18.928 8.103 15.702 1.00 10.75 O
31 CB GLU A of atom, 3-20.262 8.446 18.674 1.00 13.18 C
32 CG GLU A of atom, 3-19.216 7.406 18.917 1.00 16.03 C
33 CD GLU A of atom, 3-19.799 6.185 19.575 1.00 25.79 C
34 OE1 GLU A of atom, 3-19.307 5.801 20.655 1.00 31.35 O
35 OE2 GLU A of atom, 3-20.764 5.619 19.019 1.00 25.45 O
36 N SER A of atom, 4-19.448 10.231 16.232 1.00 9.83 N
37 CA SER A of atom, 4-18.447 10.807 15.354 1.00 11.13 C
38 C SER A of atom, 4-17.035 10.540 15.865 1.00 11.57 C
39 O SER A of atom, 4-16.801 10.366 17.068 1.00 12.43 O
40 CB SER A of atom, 4-18.679 12.311 15.259 1.00 11.73 C
41 OG SER A of atom, 4-19.938 12.582 14.669 1.00 11.07 O
42 N ARG A of atom, 5-16.089 10.526 14.927 1.00 11.42 N
43 CA ARG A of atom, 5-14.668 10.414 15.229 1.00 11.52 C
44 C ARG A of atom, 5-14.116 11.760 15.679 1.00 12.36 C
45 O ARG A of atom, 5-14.306 12.781 15.009 1.00 15.39 O
46 CB ARG A of atom, 5-13.897 9.965 13.986 1.00 12.50 C
47 CG ARG A of atom, 5-14.543 8.836 13.189 1.00 12.46 C
48 CD ARG A of atom, 5-14.555 7.514 13.973 1.00 12.96 C
49 NE ARG A of atom, 5-13.252 7.198 14.553 1.00 12.81 N
50 CZ ARG A of atom, 5-12.238 6.679 13.865 1.00 13.25 C
51 NH1 ARG A of atom, 5-12.355 6.417 12.568 1.00 13.94 N
52 NH2 ARG A of atom, 5-11.084 6.443 14.477 1.00 15.37 N
53 N VAL A of atom, 6-13.388 11.764 16.783 1.00 10.09 N
54 CA VAL A of atom, 6-12.930 13.013 17.375 1.00 9.75 C
55 C VAL A of atom, 6-11.516 13.334 16.903 1.00 10.09 C
56 O VAL A of atom, 6-10.593 12.526 17.068 1.00 10.20 O
57 CB VAL A of atom, 6-13.001 12.937 18.904 1.00 11.36 C
58 CG1 VAL A of atom, 6-12.447 14.208 19.528 1.00 11.71 C
59 CG2 VAL A of atom, 6-14.449 12.679 19.347 1.00 13.01 C
60 N ILE A of atom, 7-11.339 14.524 16.333 1.00 9.59 N
61 CA ILE A of atom, 7-10.042 14.988 15.843 1.00 9.06 C
62 C ILE A of atom, 7-9.731 16.304 16.532 1.00 8.22 C
63 O ILE A of atom, 7-10.578 17.197 16.570 1.00 10.70 O
64 CB ILE A of atom, 7-10.034 15.180 14.312 1.00 9.15 C
65 CG1 ILE A of atom, 7-10.387 13.863 13.617 1.00 9.73 C
66 CG2 ILE A of atom, 7-8.671 15.716 13.857 1.00 9.86 C
67 CD1 ILE A of atom, 7-10.557 13.973 12.099 1.00 11.55 C
68 N LEU A of atom, 8-8.526 16.423 17.092 1.00 8.82 N
69 CA LEU A of atom, 8-8.085 17.688 17.674 1.00 8.40 C
70 C LEU A of atom, 8-7.319 18.491 16.628 1.00 8.26 C
71 O LEU A of atom, 8-6.324 18.004 16.089 1.00 10.08 O
72 CB LEU A of atom, 8-7.161 17.405 18.852 1.00 9.24 C
73 CG LEU A of atom, 8-6.561 18.648 19.503 1.00 9.64 C
74 CD1 LEU A of atom, 8-7.651 19.488 20.156 1.00 12.16 C
75 CD2 LEU A of atom, 8-5.503 18.265 20.539 1.00 11.34 C
76 N ALA A of atom, 9-7.781 19.705 16.332 1.00 8.28 N
77 CA ALA A of atom, 9-7.066 20.623 15.440 1.00 8.12 C
78 C ALA A of atom, 9-6.344 21.640 16.322 1.00 8.30 C
79 O ALA A of atom, 9-6.948 22.594 16.808 1.00 9.31 O
80 CB ALA A of atom, 9-8.038 21.287 14.465 1.00 9.25 C
81 N PHE A of atom, 10-5.047 21.416 16.554 1.00 7.56 N
82 CA PHE A of atom, 10-4.265 22.179 17.527 1.00 7.68 C
83 C PHE A of atom, 10-3.337 23.106 16.748 1.00 8.07 C
84 O PHE A of atom, 10-2.350 22.647 16.170 1.00 9.14 O
85 CB PHE A of atom, 10-3.477 21.205 18.407 1.00 8.54 C
86 CG PHE A of atom, 10-2.834 21.846 19.604 1.00 7.78 C
87 CD1 PHE A of atom, 10-3.601 22.218 20.699 1.00 9.35 C
88 CD2 PHE A of atom, 10-1.461 22.069 19.645 1.00 8.05 C
89 CE1 PHE A of atom, 10-3.012 22.796 21.831 1.00 9.54 C
90 CE2 PHE A of atom, 10-0.862 22.645 20.773 1.00 9.72 C
91 CZ PHE A of atom, 10-1.640 23.017 21.861 1.00 9.66 C
92 N GLY A of atom, 11-3.658 24.395 16.706 1.00 8.30 N
93 CA GLY A of atom, 11-2.914 25.300 15.861 1.00 8.28 C
94 C GLY A of atom, 11-3.099 26.755 16.204 1.00 7.97 C
95 O GLY A of atom, 11-3.444 27.109 17.333 1.00 8.56 O
96 N ASP A of atom, 12-2.840 27.598 15.205 1.00 9.02 N
97 CA ASP A of atom, 12-2.819 29.042 15.388 1.00 9.30 C
98 C ASP A of atom, 12-3.929 29.699 14.572 1.00 9.96 C
99 O ASP A of atom, 12-5.020 29.130 14.453 1.00 10.25 O
100 CB ASP A of atom, 12-1.420 29.621 15.119 1.00 9.85 C
101 CG ASP A of atom, 12-0.887 29.257 13.757 1.00 10.69 C
102 OD1 ASP A of atom, 12-1.707 29.077 12.825 1.00 10.57 O
103 OD2 ASP A of atom, 12 0.347 29.179 13.591 1.00 10.54 O
104 N ASER A of atom, 13-3.676 30.882 14.009 0.52 10.62 N
105 CA ASER A of atom, 13-4.723 31.579 13.265 0.52 12.26 C
106 C ASER A of atom, 13-5.175 30.818 12.025 0.52 11.74 C
107 O ASER A of atom, 13-6.287 31.062 11.537 0.52 12.93 O
108 CB ASER A of atom, 13-4.266 32.986 12.876 0.52 16.09 C
109 OG ASER A of atom, 13-3.065 32.943 12.127 0.52 19.42 O
110 N BSER A of atom, 13-3.668 30.884 14.009 0.48 10.66 N
111 CA BSER A of atom, 13-4.697 31.589 13.245 0.48 12.29 C
112 C BSER A of atom, 13-5.200 30.767 12.068 0.48 11.72 C
113 O BSER A of atom, 13-6.363 30.914 11.662 0.48 13.20 O
114 CB BSER A of atom, 13-4.164 32.930 12.737 0.48 16.13 C
115 OG BSER A of atom, 13-3.942 33.838 13.799 0.48 15.63 O
116 N LEU A of atom, 14-4.348 29.911 11.497 1.00 10.58 N
117 CA LEU A of atom, 14-4.760 29.126 10.338 1.00 11.76 C
118 C LEU A of atom, 14-5.799 28.076 10.692 1.00 10.98 C
119 O LEU A of atom, 14-6.521 27.609 9.800 1.00 13.94 O
120 CB LEU A of atom, 14-3.556 28.458 9.682 1.00 13.48 C
121 CG LEU A of atom, 14-2.444 29.390 9.206 1.00 16.19 C
122 CD1 LEU A of atom, 14-1.297 28.584 8.603 1.00 16.99 C
123 CD2 LEU A of atom, 14-2.961 30.410 8.189 1.00 18.22 C
124 N PHE A of atom, 15-5.868 27.661 11.956 1.00 9.59 N
125 CA PHE A of atom, 15-6.958 26.817 12.416 1.00 10.07 C
126 C PHE A of atom, 15-8.080 27.620 13.055 1.00 11.19 C
127 O PHE A of atom, 15-9.246 27.258 12.895 1.00 12.38 O
128 CB PHE A of atom, 15-6.449 25.769 13.412 1.00 10.09 C
129 CG PHE A of atom, 15-5.767 24.584 12.771 1.00 9.26 C
130 CD1 PHE A of atom, 15-5.569 24.506 11.395 1.00 10.69 C
131 CD2 PHE A of atom, 15-5.311 23.545 13.564 1.00 9.46 C
132 CE1 PHE A of atom, 15-4.941 23.397 10.831 1.00 11.45 C
133 CE2 PHE A of atom, 15-4.687 22.443 13.016 1.00 10.15 C
134 CZ PHE A of atom, 15-4.508 22.363 11.641 1.00 10.77 C
135 N ALA A of atom, 16-7.760 28.716 13.759 1.00 11.29 N
136 CA ALA A of atom, 16-8.800 29.516 14.405 1.00 11.74 C
137 C ALA A of atom, 16-9.748 30.157 13.399 1.00 14.30 C
138 O ALA A of atom, 16-10.896 30.453 13.747 1.00 17.14 O
139 CB ALA A of atom, 16-8.176 30.599 15.284 1.00 12.72 C
140 N GLY A of atom, 17-9.296 30.397 12.171 1.00 13.40 N
141 CA GLY A of atom, 17-10.141 31.030 11.175 1.00 14.78 C
142 C GLY A of atom, 17-10.111 32.544 11.238 1.00 17.09 C
143 O GLY A of atom, 17-11.139 33.199 11.031 1.00 16.87 O
144 N TYR A of atom, 18-8.935 33.112 11.497 1.00 17.49 N
145 CA TYR A of atom, 18-8.805 34.557 11.632 1.00 17.26 C
146 C TYR A of atom, 18-9.291 35.261 10.371 1.00 20.22 C
147 O TYR A of atom, 18-8.893 34.910 9.255 1.00 19.20 O
148 CB TYR A of atom, 18-7.341 34.919 11.894 1.00 22.05 C
149 CG TYR A of atom, 18-7.062 36.401 11.768 1.00 28.04 C
150 CD1 TYR A of atom, 18-7.405 37.280 12.786 1.00 31.28 C
151 CD2 TYR A of atom, 18-6.469 36.921 10.628 1.00 28.39 C
152 CE1 TYR A of atom, 18-7.159 38.635 12.671 1.00 25.77 C
153 CE2 TYR A of atom, 18-6.219 38.274 10.504 1.00 33.60 C
154 CZ TYR A of atom, 18-6.563 39.125 11.528 1.00 32.23 C
155 OH TYR A of atom, 18-6.311 40.474 11.403 1.00 39.37 O
156 N GLY A of atom, 19-10.148 36.267 10.556 1.00 18.60 N
157 CA GLY A of atom, 19-10.689 37.044 9.458 1.00 19.65 C
158 C GLY A of atom, 19-11.837 36.398 8.723 1.00 19.43 C
159 O GLY A of atom, 19-12.351 36.993 7.765 1.00 20.82 O
160 N LEU A of atom, 20-12.265 35.211 9.134 1.00 18.55 N
161 CA LEU A of atom, 20-13.314 34.485 8.437 1.00 20.13 C
162 C LEU A of atom, 20-14.585 34.453 9.273 1.00 23.41 C
163 O LEU A of atom, 20-14.575 34.701 10.483 1.00 25.09 O
164 CB LEU A of atom, 20-12.871 33.054 8.112 1.00 17.00 C
165 CG LEU A of atom, 20-11.585 32.949 7.298 1.00 15.79 C
166 CD1 LEU A of atom, 20-11.214 31.494 7.111 1.00 16.08 C
167 CD2 LEU A of atom, 20-11.739 33.653 5.954 1.00 18.80 C
168 N ASP A of atom, 21-15.689 34.142 8.602 1.00 25.57 N
169 CA ASP A of atom, 21-16.980 34.059 9.260 1.00 25.67 C
170 C ASP A of atom, 21-17.167 32.673 9.868 1.00 25.22 C
171 O ASP A of atom, 21-16.334 31.774 9.710 1.00 24.22 O
172 CB ASP A of atom, 21-18.100 34.424 8.288 1.00 33.33 C
173 CG ASP A of atom, 21-18.101 35.898 7.929 1.00 41.76 C
174 OD1 ASP A of atom, 21-17.477 36.693 8.666 1.00 48.45 O
175 OD2 ASP A of atom, 21-18.724 36.264 6.911 1.00 44.13 O
176 N LYS A of atom, 22-18.279 32.501 10.576 1.00 26.79 N
177 CA LYS A of atom, 22-18.532 31.250 11.274 1.00 29.26 C
178 C LYS A of atom, 22-18.669 30.105 10.281 1.00 26.64 C
179 O LYS A of atom, 22-19.364 30.220 9.267 1.00 29.36 O
180 CB LYS A of atom, 22-19.800 31.373 12.120 1.00 32.19 C
181 CG LYS A of atom, 22-19.903 30.352 13.247 1.00 41.27 C
182 CD LYS A of atom, 22-18.553 30.110 13.919 1.00 44.86 C
183 CE LYS A of atom, 22-18.120 31.301 14.767 1.00 56.10 C
184 NZ LYS A of atom, 22-16.872 31.010 15.525 1.00 62.39 N
185 N GLY A of atom, 23-17.985 29.003 10.568 1.00 20.94 N
186 CA GLY A of atom, 23-18.034 27.844 9.713 1.00 18.99 C
187 C GLY A of atom, 23-17.086 27.864 8.534 1.00 14.68 C
188 O GLY A of atom, 23-17.043 26.881 7.787 1.00 17.98 O
189 N GLU A of atom, 24-16.317 28.938 8.346 1.00 15.50 N
190 CA GLU A of atom, 24-15.393 29.034 7.225 1.00 13.08 C
191 C GLU A of atom, 24-13.964 28.621 7.568 1.00 12.66 C
192 O GLU A of atom, 24-13.136 28.523 6.658 1.00 12.59 O
193 CB GLU A of atom, 24-15.385 30.461 6.670 1.00 17.05 C
194 CG GLU A of atom, 24-16.711 30.885 6.046 1.00 17.59 C
195 CD GLU A of atom, 24-16.694 32.310 5.520 1.00 22.67 C
196 OE1 GLU A of atom, 24-15.889 33.128 6.008 1.00 20.21 O
197 OE2 GLU A of atom, 24-17.488 32.613 4.607 1.00 25.96 O
198 N SER A of atom, 25-13.652 28.365 8.838 1.00 12.48 N
199 CA SER A of atom, 25-12.286 28.003 9.194 1.00 10.79 C
200 C SER A of atom, 25-11.922 26.639 8.630 1.00 10.99 C
201 O SER A of atom, 25-12.778 25.812 8.305 1.00 11.17 O
202 CB SER A of atom, 25-12.118 27.946 10.713 1.00 14.60 C
203 OG SER A of atom, 25-12.770 26.810 11.263 1.00 13.45 O
204 N TYR A of atom, 26-10.615 26.416 8.522 1.00 10.62 N
205 CA TYR A of atom, 26-10.101 25.133 8.050 1.00 10.38 C
206 C TYR A of atom, 26-10.654 23.940 8.821 1.00 9.07 C
207 O TYR A of atom, 26-11.108 22.979 8.175 1.00 9.46 O
208 CB TYR A of atom, 26-8.565 25.177 7.963 1.00 9.99 C
209 CG TYR A of atom, 26-7.961 23.841 7.609 1.00 8.56 C
210 CD1 TYR A of atom, 26-7.620 22.931 8.603 1.00 9.30 C
211 CD2 TYR A of atom, 26-7.738 23.487 6.289 1.00 9.29 C
212 CE1 TYR A of atom, 26-7.088 21.716 8.293 1.00 9.48 C
213 CE2 TYR A of atom, 26-7.202 22.263 5.969 1.00 9.46 C
214 CZ TYR A of atom, 26-6.873 21.376 6.972 1.00 8.83 C
215 OH TYR A of atom, 26-6.337 20.138 6.699 1.00 10.24 O
216 N PRO A of atom, 27-10.654 23.918 10.164 1.00 8.87 N
217 CA PRO A of atom, 27-11.219 22.742 10.852 1.00 9.56 C
218 C PRO A of atom, 27-12.687 22.505 10.523 1.00 9.14 C
219 O PRO A of atom, 27-13.099 21.348 10.345 1.00 11.09 O
220 CB PRO A of atom, 27-10.959 23.043 12.335 1.00 10.70 C
221 CG PRO A of atom, 27-9.750 23.966 12.314 1.00 10.59 C
222 CD PRO A of atom, 27-10.011 24.844 11.117 1.00 9.67 C
223 N ALA A of atom, 28-13.482 23.573 10.429 1.00 11.18 N
224 CA ALA A of atom, 28-14.901 23.411 10.122 1.00 12.41 C
225 C ALA A of atom, 28-15.108 22.873 8.710 1.00 11.50 C
226 O ALA A of atom, 28-15.932 21.977 8.490 1.00 12.28 O
227 CB ALA A of atom, 28-15.632 24.739 10.298 1.00 13.98 C
228 N LYS A of atom, 29-14.363 23.400 7.736 1.00 9.97 N
229 CA LYS A of atom, 29-14.537 22.930 6.368 1.00 10.43 C
230 C LYS A of atom, 29-13.940 21.545 6.185 1.00 9.97 C
231 O LYS A of atom, 29-14.454 20.743 5.394 1.00 11.07 O
232 CB LYS A of atom, 29-13.926 23.936 5.391 1.00 11.18 C
233 CG LYS A of atom, 29-14.739 25.225 5.256 1.00 12.80 C
234 CD LYS A of atom, 29-16.085 24.948 4.575 1.00 17.46 C
235 CE LYS A of atom, 29-16.924 26.205 4.394 1.00 19.90 C
236 NZ LYS A of atom, 29-16.437 27.080 3.284 1.00 18.49 N
237 N LEU A of atom, 30-12.859 21.240 6.913 1.00 9.91 N
238 CA LEU A of atom, 30-12.302 19.895 6.872 1.00 9.22 C
239 C LEU A of atom, 30-13.304 18.879 7.405 1.00 9.75 C
240 O LEU A of atom, 30-13.454 17.789 6.845 1.00 10.03 O
241 CB LEU A of atom, 30-11.007 19.846 7.685 1.00 9.70 C
242 CG LEU A of atom, 30-10.344 18.485 7.881 1.00 9.82 C
243 CD1 LEU A of atom, 30-9.897 17.897 6.560 1.00 10.43 C
244 CD2 LEU A of atom, 30-9.158 18.631 8.834 1.00 10.25 C
245 N GLU A of atom, 31-13.996 19.220 8.490 1.00 9.73 N
246 CA GLU A of atom, 31-14.997 18.314 9.046 1.00 11.29 C
247 C GLU A of atom, 31-16.031 17.941 7.990 1.00 11.25 C
248 O GLU A of atom, 31-16.362 16.762 7.814 1.00 13.43 O
249 CB GLU A of atom, 31-15.661 18.972 10.255 1.00 13.29 C
250 CG GLU A of atom, 31-16.635 18.065 10.969 1.00 14.24 C
251 CD GLU A of atom, 31-17.212 18.697 12.224 1.00 13.81 C
252 OE1 GLU A of atom, 31-16.463 19.098 13.152 1.00 14.29 O
253 OE2 GLU A of atom, 31-18.448 18.798 12.296 1.00 19.44 O
254 N THR A of atom, 32-16.528 18.935 7.255 1.00 11.26 N
255 CA THR A of atom, 32-17.510 18.651 6.216 1.00 13.69 C
256 C THR A of atom, 32-16.908 17.805 5.103 1.00 12.96 C
257 O THR A of atom, 32-17.541 16.851 4.628 1.00 12.69 O
258 CB THR A of atom, 32-18.066 19.959 5.660 1.00 14.30 C
259 OG1 THR A of atom, 32-18.711 20.680 6.714 1.00 19.11 O
260 CG2 THR A of atom, 32-19.067 19.688 4.540 1.00 18.67 C
261 N ALA A of atom, 33-15.678 18.123 4.687 1.00 11.35 N
262 CA ALA A of atom, 33-15.044 17.406 3.584 1.00 11.34 C
263 C ALA A of atom, 33-14.796 15.941 3.915 1.00 10.74 C
264 O ALA A of atom, 33-14.853 15.084 3.021 1.00 11.89 O
265 CB ALA A of atom, 33-13.732 18.083 3.197 1.00 11.36 C
266 N LEU A of atom, 34-14.534 15.622 5.184 1.00 9.80 N
267 CA LEU A of atom, 34-14.276 14.235 5.533 1.00 10.15 C
268 C LEU A of atom, 34-15.486 13.334 5.296 1.00 9.87 C
269 O LEU A of atom, 34-15.309 12.121 5.153 1.00 9.59 O
270 CB LEU A of atom, 34-13.795 14.144 6.981 1.00 9.38 C
271 CG LEU A of atom, 34-12.356 14.634 7.192 1.00 10.84 C
272 CD1 LEU A of atom, 34-12.015 14.669 8.668 1.00 11.24 C
273 CD2 LEU A of atom, 34-11.343 13.783 6.444 1.00 13.16 C
274 N ARG A of atom, 35-16.699 13.898 5.254 1.00 10.18 N
275 CA ARG A of atom, 35-17.874 13.095 4.906 1.00 12.67 C
276 C ARG A of atom, 35-17.678 12.388 3.574 1.00 13.88 C
277 O ARG A of atom, 35-18.127 11.248 3.391 1.00 16.32 O
278 CB ARG A of atom, 35-19.120 13.979 4.840 1.00 14.32 C
279 CG ARG A of atom, 35-19.604 14.529 6.167 1.00 19.57 C
280 CD ARG A of atom, 35-20.853 15.381 5.946 1.00 26.90 C
281 NE ARG A of atom, 35-20.868 16.575 6.784 1.00 33.65 N
282 CZ ARG A of atom, 35-21.670 17.615 6.585 1.00 37.12 C
283 NH1 ARG A of atom, 35-22.527 17.609 5.572 1.00 41.74 N
284 NH2 ARG A of atom, 35-21.617 18.659 7.400 1.00 46.45 N
285 N SER A of atom, 36-16.982 13.036 2.641 1.00 12.10 N
286 CA SER A of atom, 36-16.747 12.460 1.325 1.00 13.80 C
287 C SER A of atom, 36-15.702 11.362 1.359 1.00 13.62 C
288 O SER A of atom, 36-15.495 10.691 0.338 1.00 17.30 O
289 CB SER A of atom, 36-16.336 13.563 0.346 1.00 16.82 C
290 OG SER A of atom, 36-17.394 14.495 0.177 1.00 21.01 O
291 N HIS A of atom, 37-15.078 11.153 2.511 1.00 11.37 N
292 CA HIS A of atom, 37-14.122 10.068 2.700 1.00 12.81 C
293 C HIS A of atom, 37-14.756 8.970 3.557 1.00 12.54 C
294 O HIS A of atom, 37-14.055 8.117 4.085 1.00 14.49 O
295 CB HIS A of atom, 37-12.840 10.575 3.361 1.00 13.57 C
296 CG HIS A of atom, 37-12.075 11.536 2.515 1.00 13.44 C
297 ND1 HIS A of atom, 37-10.916 11.188 1.856 1.00 16.12 N
298 CD2 HIS A of atom, 37-12.325 12.824 2.186 1.00 14.64 C
299 CE1 HIS A of atom, 37-10.475 12.229 1.176 1.00 14.01 C
300 NE2 HIS A of atom, 37-11.310 13.233 1.356 1.00 15.55 N
301 N GLY A of atom, 38-16.077 9.026 3.728 1.00 11.83 N
302 CA GLY A of atom, 38-16.771 8.069 4.569 1.00 13.01 C
303 C GLY A of atom, 38-16.602 8.282 6.058 1.00 12.55 C
304 O GLY A of atom, 38-16.815 7.342 6.826 1.00 14.90 O
305 N ILE A of atom, 39-16.243 9.493 6.497 1.00 11.73 N
306 CA ILE A of atom, 39-15.917 9.775 7.894 1.00 11.88 C
307 C ILE A of atom, 39-16.861 10.840 8.424 1.00 11.16 C
308 O ILE A of atom, 39-16.977 11.924 7.844 1.00 11.31 O
309 CB ILE A of atom, 39-14.456 10.246 8.047 1.00 11.31 C
310 CG1 ILE A of atom, 39-13.497 9.122 7.667 1.00 12.03 C
311 CG2 ILE A of atom, 39-14.191 10.749 9.477 1.00 13.02 C
312 CD1 ILE A of atom, 39-12.087 9.605 7.332 1.00 14.48 C
313 N ASN A of atom, 40-17.510 10.542 9.557 1.00 11.25 N
314 CA ASN A of atom, 40-18.223 11.535 10.348 1.00 11.79 C
315 C ASN A of atom, 40-17.297 11.927 11.493 1.00 11.71 C
316 O ASN A of atom, 40-17.099 11.146 12.434 1.00 10.38 O
317 CB ASN A of atom, 40-19.527 10.962 10.899 1.00 13.35 C
318 CG ASN A of atom, 40-20.639 10.964 9.874 1.00 13.90 C
319 OD1 ASN A of atom, 40-20.834 11.945 9.157 1.00 20.53 O
320 ND2 ASN A of atom, 40-21.373 9.858 9.796 1.00 14.77 N
321 N ALA A of atom, 41-16.728 13.126 11.409 1.00 11.85 N
322 CA ALA A of atom, 41-15.793 13.628 12.403 1.00 11.21 C
323 C ALA A of atom, 41-16.415 14.779 13.179 1.00 13.36 C
324 O ALA A of atom, 41-17.291 15.496 12.680 1.00 15.55 O
325 CB ALA A of atom, 41-14.503 14.109 11.729 1.00 16.15 C
326 N ARG A of atom, 42-15.974 14.927 14.421 1.00 10.59 N
327 CA ARG A of atom, 42-16.175 16.132 15.209 1.00 10.99 C
328 C ARG A of atom, 42-14.778 16.682 15.412 1.00 11.21 C
329 O ARG A of atom, 42-13.955 16.054 16.091 1.00 11.68 O
330 CB ARG A of atom, 42-16.804 15.813 16.562 1.00 12.24 C
331 CG ARG A of atom, 42-16.941 17.032 17.442 1.00 14.06 C
332 CD ARG A of atom, 42-17.801 16.774 18.678 1.00 18.79 C
333 NE ARG A of atom, 42-17.168 15.945 19.708 1.00 17.21 N
334 CZ ARG A of atom, 42-16.253 16.379 20.574 1.00 20.51 C
335 NH1 ARG A of atom, 42-15.830 17.636 20.528 1.00 19.19 N
336 NH2 ARG A of atom, 42-15.768 15.555 21.497 1.00 17.67 N
337 N ILE A of atom, 43-14.487 17.810 14.788 1.00 10.15 N
338 CA ILE A of atom, 43-13.153 18.381 14.867 1.00 10.86 C
339 C ILE A of atom, 43-13.169 19.482 15.908 1.00 12.36 C
340 O ILE A of atom, 43-13.877 20.488 15.761 1.00 14.83 O
341 CB ILE A of atom, 43-12.654 18.874 13.506 1.00 11.39 C
342 CG1 ILE A of atom, 43-12.703 17.717 12.504 1.00 11.46 C
343 CG2 ILE A of atom, 43-11.228 19.414 13.653 1.00 11.27 C
344 CD1 ILE A of atom, 43-12.003 17.998 11.183 1.00 11.61 C
345 N ILE A of atom, 44-12.389 19.270 16.968 1.00 9.86 N
346 CA ILE A of atom, 44-12.194 20.251 18.027 1.00 10.61 C
347 C ILE A of atom, 44-11.309 21.353 17.470 1.00 9.46 C
348 O ILE A of atom, 44-10.146 21.113 17.116 1.00 11.41 O
349 CB ILE A of atom, 44-11.498 19.610 19.235 1.00 11.48 C
350 CG1 ILE A of atom, 44-12.210 18.355 19.728 1.00 13.63 C
351 CG2 ILE A of atom, 44-11.320 20.629 20.353 1.00 13.46 C
352 CD1 ILE A of atom, 44-11.257 17.423 20.455 1.00 14.90 C
353 N ASN A of atom, 45-11.836 22.565 17.398 1.00 10.86 N
354 CA ASN A of atom, 45-11.035 23.682 16.912 1.00 9.72 C
355 C ASN A of atom, 45-10.284 24.257 18.101 1.00 10.79 C
356 O ASN A of atom, 45-10.818 25.066 18.865 1.00 13.36 O
357 CB ASN A of atom, 45-11.883 24.732 16.207 1.00 12.13 C
358 CG ASN A of atom, 45-11.028 25.733 15.468 1.00 11.80 C
359 OD1 ASN A of atom, 45-9.875 25.965 15.856 1.00 13.53 O
360 ND2 ASN A of atom, 45-11.556 26.316 14.404 1.00 12.09 N
361 N ALA A of atom, 46-9.039 23.811 18.267 1.00 10.20 N
362 CA ALA A of atom, 46-8.134 24.358 19.267 1.00 10.25 C
363 C ALA A of atom, 46-7.113 25.288 18.629 1.00 11.04 C
364 O ALA A of atom, 46-5.955 25.346 19.053 1.00 11.43 O
365 CB ALA A of atom, 46-7.478 23.251 20.088 1.00 11.08 C
366 N GLY A of atom, 47-7.525 26.012 17.593 1.00 9.89 N
367 CA GLY A of atom, 47-6.692 27.059 17.050 1.00 10.26 C
368 C GLY A of atom, 47-6.794 28.315 17.896 1.00 10.79 C
369 O GLY A of atom, 47-7.877 28.717 18.332 1.00 12.90 O
370 N VAL A of atom, 48-5.639 28.916 18.165 1.00 10.15 N
371 CA VAL A of atom, 48-5.574 30.165 18.909 1.00 11.29 C
372 C VAL A of atom, 48-4.730 31.126 18.093 1.00 10.80 C
373 O VAL A of atom, 48-3.548 30.862 17.839 1.00 10.86 O
374 CB VAL A of atom, 48-4.982 29.983 20.315 1.00 11.83 C
375 CG1 VAL A of atom, 48-4.817 31.339 20.996 1.00 14.10 C
376 CG2 VAL A of atom, 48-5.881 29.078 21.138 1.00 13.07 C
377 N SER A of atom, 49-5.324 32.239 17.685 1.00 11.85 N
378 CA SER A of atom, 49-4.607 33.195 16.857 1.00 13.79 C
379 C SER A of atom, 49-3.393 33.721 17.611 1.00 13.73 C
380 O SER A of atom, 49-3.472 34.038 18.800 1.00 15.81 O
381 CB SER A of atom, 49-5.536 34.340 16.465 1.00 15.44 C
382 OG SER A of atom, 49-6.521 33.866 15.556 1.00 19.05 O
383 N GLY A of atom, 50-2.259 33.784 16.915 1.00 16.09 N
384 CA GLY A of atom, 50-1.031 34.276 17.504 1.00 17.30 C
385 C GLY A of atom, 50-0.225 33.284 18.319 1.00 14.10 C
386 O GLY A of atom, 50 0.868 33.643 18.781 1.00 15.63 O
387 N ASP A of atom, 51-0.711 32.059 18.514 1.00 11.53 N
388 CA ASP A of atom, 51 0.030 31.084 19.308 1.00 10.34 C
389 C ASP A of atom, 51 1.380 30.763 18.687 1.00 9.45 C
390 O ASP A of atom, 51 1.461 30.355 17.530 1.00 10.15 O
391 CB ASP A of atom, 51-0.753 29.775 19.414 1.00 11.28 C
392 CG ASP A of atom, 51-1.389 29.561 20.771 1.00 12.58 C
393 OD1 ASP A of atom, 51-1.572 30.539 21.534 1.00 13.07 O
394 OD2 ASP A of atom, 51-1.652 28.389 21.096 1.00 15.49 O
395 N THR A of atom, 52 2.432 30.907 19.480 1.00 9.65 N
396 CA THR A of atom, 52 3.745 30.388 19.130 1.00 10.06 C
397 C THR A of atom, 52 3.827 28.917 19.527 1.00 9.29 C
398 O THR A of atom, 52 2.944 28.381 20.197 1.00 9.01 O
399 CB THR A of atom, 52 4.810 31.126 19.930 1.00 11.39 C
400 OG1 THR A of atom, 52 4.501 30.966 21.319 1.00 10.69 O
401 CG2 THR A of atom, 52 4.837 32.617 19.572 1.00 12.95 C
402 N THR A of atom, 53 4.927 28.260 19.141 1.00 9.44 N
403 CA THR A of atom, 53 5.159 26.907 19.634 1.00 8.52 C
404 C THR A of atom, 53 5.211 26.869 21.161 1.00 7.86 C
405 O THR A of atom, 53 4.715 25.922 21.776 1.00 9.32 O
406 CB THR A of atom, 53 6.428 26.308 19.009 1.00 9.18 C
407 OG1 THR A of atom, 53 7.586 27.073 19.402 1.00 9.52 O
408 CG2 THR A of atom, 53 6.317 26.205 17.477 1.00 9.88 C
409 N ALA A of atom, 54 5.812 27.887 21.795 1.00 9.82 N
410 CA ALA A of atom, 54 5.850 27.904 23.257 1.00 8.56 C
411 C ALA A of atom, 54 4.448 28.036 23.837 1.00 8.45 C
412 O ALA A of atom, 54 4.119 27.376 24.828 1.00 8.87 O
413 CB ALA A of atom, 54 6.741 29.036 23.775 1.00 10.02 C
414 N ALA A of atom, 55 3.612 28.891 23.239 1.00 8.18 N
415 CA ALA A of atom, 55 2.235 29.003 23.717 1.00 9.53 C
416 C ALA A of atom, 55 1.487 27.680 23.570 1.00 7.93 C
417 O ALA A of atom, 55 0.728 27.275 24.462 1.00 9.32 O
418 CB ALA A of atom, 55 1.507 30.138 22.992 1.00 11.09 C
419 N GLY A of atom, 56 1.696 26.982 22.452 1.00 8.96 N
420 CA GLY A of atom, 56 1.059 25.688 22.286 1.00 9.29 C
421 C GLY A of atom, 56 1.530 24.686 23.318 1.00 8.08 C
422 O GLY A of atom, 56 0.733 23.939 23.886 1.00 9.42 O
423 N LEU A of atom, 57 2.837 24.659 23.581 1.00 9.10 N
424 CA LEU A of atom, 57 3.360 23.758 24.602 1.00 9.10 C
425 C LEU A of atom, 57 2.769 24.068 25.972 1.00 8.84 C
426 O LEU A of atom, 57 2.481 23.158 26.753 1.00 10.95 O
427 CB LEU A of atom, 57 4.885 23.841 24.623 1.00 9.77 C
428 CG LEU A of atom, 57 5.610 23.010 25.684 1.00 10.84 C
429 CD1 LEU A of atom, 57 5.276 21.531 25.537 1.00 12.43 C
430 CD2 LEU A of atom, 57 7.107 23.255 25.575 1.00 14.05 C
431 N GLN A of atom, 58 2.557 25.350 26.259 1.00 8.54 N
432 CA GLN A of atom, 58 2.030 25.748 27.556 1.00 9.32 C
433 C GLN A of atom, 58 0.616 25.237 27.780 1.00 9.41 C
434 O GLN A of atom, 58 0.224 24.997 28.931 1.00 10.86 O
435 CB GLN A of atom, 58 2.049 27.266 27.663 1.00 10.43 C
436 CG GLN A of atom, 58 1.759 27.775 29.072 1.00 11.92 C
437 CD GLN A of atom, 58 2.907 27.494 30.022 1.00 13.61 C
438 OE1 GLN A of atom, 58 4.072 27.603 29.644 1.00 16.64 O
439 NE2 GLN A of atom, 58 2.589 27.131 31.252 1.00 19.37 N
440 N ARG A of atom, 59-0.163 25.046 26.711 1.00 9.57 N
441 CA ARG A of atom, 59-1.572 24.695 26.847 1.00 8.79 C
442 C ARG A of atom, 59-1.923 23.281 26.409 1.00 9.71 C
443 O ARG A of atom, 59-3.077 22.883 26.593 1.00 10.41 O
444 CB ARG A of atom, 59-2.495 25.715 26.156 1.00 8.95 C
445 CG ARG A of atom, 59-2.385 25.744 24.654 1.00 10.27 C
446 CD ARG A of atom, 59-3.452 26.657 24.016 1.00 10.62 C
447 NE ARG A of atom, 59-3.141 26.816 22.600 1.00 10.32 N
448 CZ ARG A of atom, 59-3.847 26.298 21.599 1.00 10.89 C
449 NH1 ARG A of atom, 59-4.973 25.644 21.828 1.00 11.19 N
450 NH2 ARG A of atom, 59-3.421 26.456 20.358 1.00 10.77 N
451 N ILE A of atom, 60-0.988 22.497 25.856 1.00 9.44 N
452 CA ILE A of atom, 60-1.393 21.213 25.275 1.00 9.33 C
453 C ILE A of atom, 60-2.024 20.287 26.317 1.00 8.62 C
454 O ILE A of atom, 60-3.042 19.639 26.049 1.00 9.97 O
455 CB ILE A of atom, 60-0.255 20.548 24.477 1.00 9.44 C
456 CG1 ILE A of atom, 60-0.737 19.221 23.867 1.00 9.97 C
457 CG2 ILE A of atom, 60 0.988 20.296 25.344 1.00 10.76 C
458 CD1 ILE A of atom, 60-1.840 19.348 22.799 1.00 11.01 C
459 N ALYS A of atom, 61-1.433 20.201 27.510 0.44 9.90 N
460 CA ALYS A of atom, 61-2.001 19.326 28.529 0.44 10.03 C
461 C ALYS A of atom, 61-3.396 19.783 28.937 0.44 9.02 C
462 O ALYS A of atom, 61-4.313 18.963 29.064 0.44 9.96 O
463 CB ALYS A of atom, 61-1.077 19.266 29.740 0.44 11.66 C
464 CG ALYS A of atom, 61-1.587 18.384 30.852 0.44 14.06 C
465 CD ALYS A of atom, 61-0.653 18.412 32.047 0.44 15.32 C
466 CE ALYS A of atom, 61-1.393 18.047 33.324 0.44 16.99 C
467 NZ ALYS A of atom, 61-2.264 16.855 33.128 0.44 16.46 N
468 N BLYS A of atom, 61-1.429 20.193 27.510 0.56 9.81 N
469 CA BLYS A of atom, 61-1.999 19.326 28.539 0.56 10.03 C
470 C BLYS A of atom, 61-3.401 19.783 28.926 0.56 8.98 C
471 O BLYS A of atom, 61-4.324 18.968 29.036 0.56 9.92 O
472 CB BLYS A of atom, 61-1.093 19.296 29.768 0.56 11.66 C
473 CG BLYS A of atom, 61 0.118 18.370 29.671 0.56 11.85 C
474 CD BLYS A of atom, 61 0.971 18.487 30.942 0.56 16.23 C
475 CE BLYS A of atom, 61 2.184 17.566 30.931 0.56 19.55 C
476 NZ BLYS A of atom, 61 1.808 16.130 31.001 0.56 18.26 N
477 N PHE A of atom, 62-3.574 21.088 29.142 1.00 9.91 N
478 CA PHE A of atom, 62-4.887 21.615 29.507 1.00 9.45 C
479 C PHE A of atom, 62-5.922 21.326 28.428 1.00 9.81 C
480 O PHE A of atom, 62-7.055 20.934 28.729 1.00 9.85 O
481 CB PHE A of atom, 62-4.784 23.123 29.731 1.00 10.72 C
482 CG PHE A of atom, 62-6.093 23.773 30.090 1.00 10.80 C
483 CD1 PHE A of atom, 62-6.537 23.775 31.403 1.00 12.96 C
484 CD2 PHE A of atom, 62-6.888 24.364 29.128 1.00 14.10 C
485 CE1 PHE A of atom, 62-7.737 24.368 31.753 1.00 16.09 C
486 CE2 PHE A of atom, 62-8.100 24.959 29.480 1.00 16.73 C
487 CZ PHE A of atom, 62-8.524 24.953 30.791 1.00 16.87 C
488 N VAL A of atom, 63-5.561 21.546 27.164 1.00 8.93 N
489 CA VAL A of atom, 63-6.502 21.302 26.071 1.00 8.06 C
490 C VAL A of atom, 63-6.906 19.833 26.041 1.00 8.94 C
491 O VAL A of atom, 63-8.090 19.499 25.904 1.00 10.43 O
492 CB VAL A of atom, 63-5.899 21.771 24.731 1.00 8.72 C
493 CG1 VAL A of atom, 63-6.723 21.269 23.560 1.00 10.55 C
494 CG2 VAL A of atom, 63-5.802 23.288 24.698 1.00 10.01 C
495 N LEU A of atom, 64-5.939 18.932 26.196 1.00 8.89 N
496 CA LEU A of atom, 64-6.264 17.507 26.196 1.00 10.17 C
497 C LEU A of atom, 64-7.114 17.131 27.405 1.00 10.35 C
498 O LEU A of atom, 64-8.107 16.404 27.275 1.00 10.95 O
499 CB LEU A of atom, 64-4.979 16.689 26.171 1.00 9.39 C
500 CG LEU A of atom, 64-4.228 16.777 24.842 1.00 9.68 C
501 CD1 LEU A of atom, 64-2.828 16.189 24.969 1.00 11.80 C
502 CD2 LEU A of atom, 64-5.010 16.083 23.714 1.00 10.27 C
503 N ASP A of atom, 65-6.747 17.625 28.590 1.00 9.14 N
504 CA ASP A of atom, 65-7.493 17.316 29.810 1.00 9.07 C
505 C ASP A of atom, 65-8.888 17.913 29.787 1.00 10.26 C
506 O ASP A of atom, 65-9.752 17.470 30.556 1.00 13.17 O
507 CB ASP A of atom, 65-6.742 17.852 31.040 1.00 11.28 C
508 CG ASP A of atom, 65-5.459 17.100 31.339 1.00 11.59 C
509 OD1 ASP A of atom, 65-5.224 16.000 30.799 1.00 12.67 O
510 OD2 ASP A of atom, 65-4.671 17.612 32.153 1.00 13.55 O
511 N SER A of atom, 66-9.123 18.925 28.955 1.00 10.73 N
512 CA SER A of atom, 66-10.435 19.545 28.868 1.00 11.21 C
513 C SER A of atom, 66-11.407 18.757 28.011 1.00 12.68 C
514 O SER A of atom, 66-12.601 19.080 28.018 1.00 15.16 O
515 CB SER A of atom, 66-10.328 20.965 28.308 1.00 12.52 C
516 OG SER A of atom, 66-9.645 21.800 29.219 1.00 13.88 O
517 N GLN A of atom, 67-10.939 17.747 27.289 1.00 9.98 N
518 CA GLN A of atom, 67-11.770 17.065 26.303 1.00 11.63 C
519 C GLN A of atom, 67-12.642 16.009 26.966 1.00 12.79 C
520 O GLN A of atom, 67-12.120 15.133 27.659 1.00 13.02 O
521 CB GLN A of atom, 67-10.884 16.381 25.272 1.00 11.54 C
522 CG GLN A of atom, 67-10.079 17.352 24.444 1.00 11.64 C
523 CD GLN A of atom, 67-10.925 18.500 23.943 1.00 12.22 C
524 OE1 GLN A of atom, 67-12.017 18.296 23.398 1.00 13.37 O
525 NE2 GLN A of atom, 67-10.437 19.715 24.121 1.00 12.68 N
526 N PRO A of atom, 68-13.958 16.024 26.737 1.00 12.92 N
527 CA PRO A of atom, 68-14.778 14.900 27.215 1.00 13.15 C
528 C PRO A of atom, 68-14.457 13.613 26.495 1.00 12.46 C
529 O PRO A of atom, 68-14.555 12.535 27.092 1.00 14.51 O
530 CB PRO A of atom, 68-16.217 15.353 26.927 1.00 16.97 C
531 CG PRO A of atom, 68-16.131 16.842 26.742 1.00 23.94 C
532 CD PRO A of atom, 68-14.769 17.097 26.152 1.00 13.67 C
533 N ASP A of atom, 69-14.069 13.692 25.221 1.00 12.42 N
534 CA ASP A of atom, 69-13.718 12.523 24.432 1.00 12.04 C
535 C ASP A of atom, 69-12.274 12.649 23.976 1.00 12.50 C
536 O ASP A of atom, 69-11.894 13.657 23.370 1.00 11.69 O
537 CB ASP A of atom, 69-14.599 12.410 23.189 1.00 15.15 C
538 CG ASP A of atom, 69-16.065 12.302 23.518 1.00 17.65 C
539 OD1 ASP A of atom, 69-16.421 11.456 24.372 1.00 15.31 O
540 OD2 ASP A of atom, 69-16.853 13.064 22.909 1.00 19.72 O
541 N LYS A of atom, 70-11.487 11.625 24.235 1.00 12.07 N
542 CA LYS A of atom, 70-10.081 11.657 23.874 1.00 12.05 C
543 C LYS A of atom, 70-9.934 11.742 22.357 1.00 11.82 C
544 O LYS A of atom, 70-10.582 10.976 21.625 1.00 11.84 O
545 CB LYS A of atom, 70-9.418 10.380 24.367 1.00 14.87 C
546 CG LYS A of atom, 70-7.929 10.342 24.159 1.00 14.25 C
547 CD LYS A of atom, 70-7.356 9.055 24.727 1.00 18.79 C
548 CE LYS A of atom, 70-5.850 9.089 24.699 1.00 18.37 C
549 NZ LYS A of atom, 70-5.278 7.861 25.326 1.00 22.73 N
550 N PRO A of atom, 71-9.094 12.637 21.847 1.00 9.83 N
551 CA PRO A of atom, 71-8.894 12.709 20.397 1.00 9.98 C
552 C PRO A of atom, 71-8.369 11.402 19.838 1.00 10.31 C
553 O PRO A of atom, 71-7.508 10.744 20.427 1.00 11.80 O
554 CB PRO A of atom, 71-7.865 13.831 20.244 1.00 12.09 C
555 CG PRO A of atom, 71-8.094 14.703 21.453 1.00 12.65 C
556 CD PRO A of atom, 71-8.394 13.719 22.559 1.00 12.02 C
557 N GLU A of atom, 72-8.891 11.043 18.672 1.00 10.24 N
558 CA GLU A of atom, 72-8.434 9.879 17.940 1.00 10.61 C
559 C GLU A of atom, 72-7.350 10.222 16.924 1.00 11.18 C
560 O GLU A of atom, 72-6.694 9.321 16.396 1.00 11.33 O
561 CB GLU A of atom, 72-9.639 9.197 17.279 1.00 11.69 C
562 CG GLU A of atom, 72-10.689 8.764 18.310 1.00 13.22 C
563 CD GLU A of atom, 72-12.008 8.329 17.692 1.00 13.37 C
564 OE1 GLU A of atom, 72-12.998 9.091 17.799 1.00 16.14 O
565 OE2 GLU A of atom, 72-12.047 7.217 17.124 1.00 15.59 O
566 N LEU A of atom, 73-7.127 11.508 16.687 1.00 10.91 N
567 CA LEU A of atom, 73-6.056 12.026 15.854 1.00 9.17 C
568 C LEU A of atom, 73-5.853 13.455 16.320 1.00 9.01 C
569 O LEU A of atom, 73-6.829 14.148 16.615 1.00 9.64 O
570 CB LEU A of atom, 73-6.467 12.045 14.375 1.00 10.14 C
571 CG LEU A of atom, 73-5.540 12.777 13.402 1.00 9.36 C
572 CD1 LEU A of atom, 73-4.197 12.079 13.294 1.00 10.03 C
573 CD2 LEU A of atom, 73-6.193 12.866 12.022 1.00 10.36 C
574 N ALA A of atom, 74-4.597 13.882 16.391 1.00 9.52 N
575 CA ALA A of atom, 74-4.257 15.267 16.683 1.00 8.97 C
576 C ALA A of atom, 74-3.519 15.829 15.481 1.00 9.41 C
577 O ALA A of atom, 74-2.501 15.263 15.057 1.00 10.68 O
578 CB ALA A of atom, 74-3.362 15.378 17.917 1.00 10.09 C
579 N ILE A of atom, 75-4.021 16.936 14.949 1.00 8.51 N
580 CA ILE A of atom, 75-3.365 17.661 13.869 1.00 8.79 C
581 C ILE A of atom, 75-2.674 18.863 14.492 1.00 9.19 C
582 O ILE A of atom, 75-3.330 19.704 15.111 1.00 10.02 O
583 CB ILE A of atom, 75-4.374 18.116 12.803 1.00 9.06 C
584 CG1 ILE A of atom, 75-5.253 16.956 12.311 1.00 10.57 C
585 CG2 ILE A of atom, 75-3.635 18.787 11.654 1.00 9.64 C
586 CD1 ILE A of atom, 75-6.384 17.389 11.375 1.00 10.50 C
587 N VAL A of atom, 76-1.358 18.960 14.328 1.00 8.60 N
588 CA VAL A of atom, 76-0.562 19.994 14.989 1.00 8.68 C
589 C VAL A of atom, 76-0.037 20.953 13.930 1.00 7.78 C
590 O VAL A of atom, 76 0.686 20.541 13.012 1.00 8.44 O
591 CB VAL A of atom, 76 0.580 19.386 15.815 1.00 8.96 C
592 CG1 VAL A of atom, 76 1.449 20.482 16.411 1.00 10.63 C
593 CG2 VAL A of atom, 76 0.029 18.471 16.896 1.00 10.86 C
594 N GLU A of atom, 77-0.376 22.236 14.080 1.00 8.60 N
595 CA GLU A of atom, 77-0.086 23.265 13.083 1.00 8.46 C
596 C GLU A of atom, 77 0.438 24.492 13.827 1.00 7.65 C
597 O GLU A of atom, 77-0.330 25.377 14.193 1.00 8.41 O
598 CB GLU A of atom, 77-1.365 23.573 12.312 1.00 10.15 C
599 CG GLU A of atom, 77-1.258 24.673 11.296 1.00 9.50 C
600 CD GLU A of atom, 77-1.650 26.039 11.839 1.00 9.41 C
601 OE1 GLU A of atom, 77-0.892 26.992 11.539 1.00 9.35 O
602 OE2 GLU A of atom, 77-2.689 26.181 12.529 1.00 10.11 O
603 N LEU A of atom, 78 1.748 24.529 14.072 1.00 8.49 N
604 CA LEU A of atom, 78 2.336 25.616 14.838 1.00 9.17 C
605 C LEU A of atom, 78 3.734 25.889 14.314 1.00 9.27 C
606 O LEU A of atom, 78 4.410 25.008 13.769 1.00 9.55 O
607 CB LEU A of atom, 78 2.426 25.295 16.330 1.00 9.32 C
608 CG LEU A of atom, 78 1.098 25.252 17.076 1.00 8.27 C
609 CD1 LEU A of atom, 78 1.344 24.738 18.487 1.00 11.70 C
610 CD2 LEU A of atom, 78 0.472 26.621 17.142 1.00 10.01 C
611 N GLY A of atom, 79 4.179 27.119 14.550 1.00 9.52 N
612 CA GLY A of atom, 79 5.507 27.578 14.177 1.00 10.88 C
613 C GLY A of atom, 79 5.491 28.812 13.310 1.00 10.33 C
614 O GLY A of atom, 79 6.483 29.557 13.279 1.00 10.98 O
615 N GLY A of atom, 80 4.383 29.066 12.615 1.00 9.68 N
616 CA GLY A of atom, 80 4.288 30.275 11.818 1.00 11.89 C
617 C GLY A of atom, 80 4.507 31.537 12.631 1.00 12.65 C
618 O GLY A of atom, 80 5.153 32.479 12.166 1.00 13.37 O
619 N ASN A of atom, 81 3.990 31.573 13.858 1.00 10.09 N
620 CA ASN A of atom, 81 4.176 32.769 14.668 1.00 11.70 C
621 C ASN A of atom, 81 5.601 32.895 15.186 1.00 12.66 C
622 O ASN A of atom, 81 6.086 34.022 15.351 1.00 15.73 O
623 CB ASN A of atom, 81 3.128 32.850 15.774 1.00 12.10 C
624 CG ASN A of atom, 81 1.782 33.251 15.233 1.00 14.94 C
625 OD1 ASN A of atom, 81 1.632 34.310 14.621 1.00 22.82 O
626 ND2 ASN A of atom, 81 0.805 32.396 15.414 1.00 15.71 N
627 N ASP A of atom, 82 6.292 31.776 15.425 1.00 9.41 N
628 CA ASP A of atom, 82 7.720 31.855 15.733 1.00 10.09 C
629 C ASP A of atom, 82 8.472 32.516 14.589 1.00 12.69 C
630 O ASP A of atom, 82 9.312 33.394 14.808 1.00 13.35 O
631 CB ASP A of atom, 82 8.313 30.468 16.021 1.00 10.09 C
632 CG ASP A of atom, 82 7.651 29.761 17.177 1.00 9.99 C
633 OD1 ASP A of atom, 82 6.403 29.656 17.168 1.00 10.99 O
634 OD2 ASP A of atom, 82 8.375 29.278 18.072 1.00 10.29 O
635 N LEU A of atom, 83 8.181 32.100 13.353 1.00 13.31 N
636 CA LEU A of atom, 83 8.731 32.762 12.169 1.00 17.40 C
637 C LEU A of atom, 83 8.465 34.259 12.199 1.00 23.03 C
638 O LEU A of atom, 83 9.370 35.075 11.981 1.00 23.93 O
639 CB LEU A of atom, 83 8.023 32.219 10.935 1.00 20.59 C
640 CG LEU A of atom, 83 8.399 30.879 10.354 1.00 22.29 C
641 CD1 LEU A of atom, 83 7.444 30.537 9.217 1.00 25.28 C
642 CD2 LEU A of atom, 83 9.824 30.935 9.865 1.00 25.44 C
643 N LEU A of atom, 84 7.206 34.637 12.417 1.00 20.62 N
644 CA LEU A of atom, 84 6.812 36.037 12.316 1.00 22.02 C
645 C LEU A of atom, 84 7.470 36.893 13.387 1.00 24.11 C
646 O LEU A of atom, 84 7.600 38.108 13.205 1.00 28.71 O
647 CB LEU A of atom, 84 5.287 36.154 12.387 1.00 23.79 C
648 CG LEU A of atom, 84 4.475 35.933 11.097 1.00 32.24 C
649 CD1 LEU A of atom, 84 5.042 34.849 10.175 1.00 30.33 C
650 CD2 LEU A of atom, 84 3.028 35.618 11.433 1.00 31.15 C
651 N ARG A of atom, 85 7.881 36.288 14.495 1.00 20.85 N
652 CA ARG A of atom, 85 8.661 36.966 15.517 1.00 17.70 C
653 C ARG A of atom, 85 10.166 36.811 15.310 1.00 20.87 C
654 O ARG A of atom, 85 10.937 37.330 16.120 1.00 24.06 O
655 CB ARG A of atom, 85 8.261 36.467 16.910 1.00 21.09 C
656 CG ARG A of atom, 85 6.844 36.847 17.361 1.00 21.56 C
657 CD ARG A of atom, 85 6.525 36.174 18.692 1.00 27.13 C
658 NE ARG A of atom, 85 5.184 36.480 19.181 1.00 28.98 N
659 CZ ARG A of atom, 85 4.899 37.503 19.979 1.00 38.84 C
660 NH1 ARG A of atom, 85 3.651 37.706 20.379 1.00 37.12 N
661 NH2 ARG A of atom, 85 5.864 38.321 20.378 1.00 32.61 N
662 N GLY A of atom, 86 10.598 36.123 14.250 1.00 18.68 N
663 CA GLY A of atom, 86 12.018 35.888 14.010 1.00 19.31 C
664 C GLY A of atom, 86 12.713 35.021 15.037 1.00 12.51 C
665 O GLY A of atom, 86 13.939 35.106 15.190 1.00 14.27 O
666 N LEU A of atom, 87 11.966 34.179 15.737 1.00 10.70 N
667 CA LEU A of atom, 87 12.491 33.427 16.864 1.00 10.68 C
668 C LEU A of atom, 87 13.321 32.236 16.395 1.00 9.32 C
669 O LEU A of atom, 87 13.192 31.750 15.263 1.00 10.25 O
670 CB LEU A of atom, 87 11.351 32.914 17.739 1.00 11.29 C
671 CG LEU A of atom, 87 10.500 34.005 18.385 1.00 12.95 C
672 CD1 LEU A of atom, 87 9.387 33.358 19.212 1.00 15.16 C
673 CD2 LEU A of atom, 87 11.345 34.942 19.238 1.00 16.17 C
674 N SER A of atom, 88 14.140 31.737 17.307 1.00 10.07 N
675 CA SER A of atom, 88 15.040 30.625 17.053 1.00 9.52 C
676 C SER A of atom, 88 14.288 29.435 16.458 1.00 8.55 C
677 O SER A of atom, 88 13.351 28.919 17.091 1.00 8.97 O
678 CB SER A of atom, 88 15.653 30.207 18.384 1.00 10.48 C
679 OG SER A of atom, 88 16.247 28.926 18.279 1.00 11.14 O
680 N PRO A of atom, 89 14.704 28.926 15.298 1.00 9.51 N
681 CA PRO A of atom, 89 14.103 27.669 14.818 1.00 9.93 C
682 C PRO A of atom, 89 14.400 26.493 15.726 1.00 9.72 C
683 O PRO A of atom, 89 13.560 25.591 15.863 1.00 9.87 O
684 CB PRO A of atom, 89 14.731 27.478 13.433 1.00 11.02 C
685 CG PRO A of atom, 89 15.154 28.855 13.019 1.00 11.64 C
686 CD PRO A of atom, 89 15.594 29.533 14.287 1.00 10.96 C
687 N ALA A of atom, 90 15.581 26.472 16.354 1.00 9.54 N
688 CA ALA A of atom, 90 15.915 25.372 17.250 1.00 10.50 C
689 C ALA A of atom, 90 14.961 25.329 18.435 1.00 9.55 C
690 O ALA A of atom, 90 14.502 24.255 18.841 1.00 11.63 O
691 CB ALA A of atom, 90 17.365 25.503 17.716 1.00 13.08 C
692 N GLU A of atom, 91 14.641 26.491 18.991 1.00 9.04 N
693 CA GLU A of atom, 91 13.702 26.523 20.103 1.00 8.72 C
694 C GLU A of atom, 91 12.297 26.141 19.660 1.00 9.08 C
695 O GLU A of atom, 91 11.568 25.499 20.419 1.00 8.94 O
696 CB GLU A of atom, 91 13.735 27.892 20.761 1.00 11.28 C
697 CG GLU A of atom, 91 15.094 28.141 21.390 1.00 11.06 C
698 CD GLU A of atom, 91 15.171 29.412 22.147 1.00 12.39 C
699 OE1 GLU A of atom, 91 14.131 30.038 22.422 1.00 16.61 O
700 OE2 GLU A of atom, 91 16.301 29.793 22.511 1.00 14.10 O
701 N ALA A of atom, 92 11.896 26.530 18.441 1.00 9.49 N
702 CA ALA A of atom, 92 10.601 26.091 17.916 1.00 9.30 C
703 C ALA A of atom, 92 10.548 24.570 17.819 1.00 9.17 C
704 O ALA A of atom, 92 9.542 23.943 18.179 1.00 9.90 O
705 CB ALA A of atom, 92 10.341 26.734 16.550 1.00 10.34 C
706 N ARG A of atom, 93 11.638 23.961 17.347 1.00 8.53 N
707 CA ARG A of atom, 93 11.717 22.505 17.277 1.00 9.91 C
708 C ARG A of atom, 93 11.590 21.874 18.657 1.00 9.28 C
709 O ARG A of atom, 93 10.899 20.862 18.825 1.00 9.51 O
710 CB ARG A of atom, 93 13.036 22.094 16.623 1.00 8.92 C
711 CG ARG A of atom, 93 13.176 20.599 16.383 1.00 11.36 C
712 CD ARG A of atom, 93 14.533 20.261 15.758 1.00 12.46 C
713 NE ARG A of atom, 93 15.606 20.760 16.602 1.00 14.00 N
714 CZ ARG A of atom, 93 16.611 21.527 16.186 1.00 15.26 C
715 NH1 ARG A of atom, 93 17.512 21.960 17.061 1.00 15.90 N
716 NH2 ARG A of atom, 93 16.726 21.855 14.906 1.00 14.85 N
717 N GLN A of atom, 94 12.281 22.443 19.653 1.00 8.43 N
718 CA GLN A of atom, 94 12.207 21.919 21.015 1.00 9.28 C
719 C GLN A of atom, 94 10.796 22.032 21.578 1.00 7.99 C
720 O GLN A of atom, 94 10.297 21.101 22.217 1.00 9.30 O
721 CB GLN A of atom, 94 13.174 22.690 21.909 1.00 12.13 C
722 CG GLN A of atom, 94 14.637 22.583 21.522 1.00 21.28 C
723 CD GLN A of atom, 94 15.456 23.788 21.992 1.00 30.86 C
724 OE1 GLN A of atom, 94 15.048 24.525 22.896 1.00 29.78 O
725 NE2 GLN A of atom, 94 16.617 23.991 21.373 1.00 29.07 N
726 N ASN A of atom, 95 10.142 23.174 21.355 1.00 8.50 N
727 CA ASN A of atom, 95 8.784 23.358 21.859 1.00 9.02 C
728 C ASN A of atom, 95 7.821 22.386 21.189 1.00 8.55 C
729 O ASN A of atom, 95 7.008 21.734 21.861 1.00 9.61 O
730 CB ASN A of atom, 95 8.339 24.802 21.630 1.00 9.16 C
731 CG ASN A of atom, 95 9.118 25.799 22.452 1.00 9.64 C
732 OD1 ASN A of atom, 95 9.722 25.448 23.475 1.00 11.85 O
733 ND2 ASN A of atom, 95 9.099 27.053 22.024 1.00 10.74 N
734 N LEU A of atom, 96 7.919 22.256 19.862 1.00 8.83 N
735 CA LEU A of atom, 96 7.082 21.289 19.161 1.00 8.50 C
736 C LEU A of atom, 96 7.354 19.870 19.639 1.00 8.05 C
737 O LEU A of atom, 96 6.427 19.058 19.739 1.00 9.48 O
738 CB LEU A of atom, 96 7.305 21.393 17.654 1.00 9.53 C
739 CG LEU A of atom, 96 6.541 22.531 16.983 1.00 10.62 C
740 CD1 LEU A of atom, 96 7.161 22.909 15.649 1.00 11.73 C
741 CD2 LEU A of atom, 96 5.069 22.185 16.804 1.00 12.67 C
742 N SER A of atom, 97 8.621 19.555 19.946 1.00 9.90 N
743 CA SER A of atom, 97 8.945 18.231 20.470 1.00 10.34 C
744 C SER A of atom, 97 8.171 17.956 21.753 1.00 9.28 C
745 O SER A of atom, 97 7.675 16.845 21.960 1.00 10.32 O
746 CB SER A of atom, 97 10.448 18.122 20.741 1.00 10.69 C
747 OG SER A of atom, 97 11.196 18.155 19.547 1.00 13.21 O
748 N GLY A of atom, 98 8.067 18.958 22.629 1.00 8.86 N
749 CA GLY A of atom, 98 7.317 18.775 23.861 1.00 9.80 C
750 C GLY A of atom, 98 5.841 18.530 23.612 1.00 9.17 C
751 O GLY A of atom, 98 5.212 17.732 24.311 1.00 10.04 O
752 N ILE A of atom, 99 5.265 19.210 22.614 1.00 9.02 N
753 CA ILE A of atom, 99 3.866 18.971 22.258 1.00 9.84 C
754 C ILE A of atom, 99 3.681 17.547 21.759 1.00 8.20 C
755 O ILE A of atom, 99 2.767 16.830 22.188 1.00 10.00 O
756 CB ILE A of atom, 99 3.391 19.984 21.206 1.00 8.78 C
757 CG1 ILE A of atom, 99 3.426 21.397 21.778 1.00 10.08 C
758 CG2 ILE A of atom, 99 1.988 19.628 20.718 1.00 9.36 C
759 CD1 ILE A of atom, 99 3.332 22.479 20.727 1.00 10.20 C
760 N LEU A of atom, 100 4.551 17.116 20.847 1.00 8.42 N
761 CA LEU A of atom, 100 4.395 15.787 20.271 1.00 10.03 C
762 C LEU A of atom, 100 4.661 14.709 21.306 1.00 10.17 C
763 O LEU A of atom, 100 4.027 13.648 21.272 1.00 11.61 O
764 CB LEU A of atom, 100 5.322 15.614 19.071 1.00 10.50 C
765 CG LEU A of atom, 100 5.119 16.634 17.944 1.00 11.10 C
766 CD1 LEU A of atom, 100 5.849 16.191 16.690 1.00 15.31 C
767 CD2 LEU A of atom, 100 3.642 16.904 17.640 1.00 14.47 C
768 N GLU A of atom, 101 5.580 14.970 22.239 1.00 11.19 N
769 CA GLU A of atom, 101 5.864 14.004 23.290 1.00 12.30 C
770 C GLU A of atom, 101 4.646 13.787 24.170 1.00 12.00 C
771 O GLU A of atom, 101 4.373 12.657 24.591 1.00 14.16 O
772 CB GLU A of atom, 101 7.060 14.470 24.119 1.00 12.87 C
773 CG GLU A of atom, 101 8.379 14.092 23.491 1.00 18.99 C
774 CD GLU A of atom, 101 9.594 14.656 24.200 1.00 30.51 C
775 OE1 GLU A of atom, 101 9.443 15.275 25.279 1.00 32.65 O
776 OE2 GLU A of atom, 101 10.708 14.470 23.661 1.00 27.86 O
777 N GLU A of atom, 102 3.909 14.853 24.474 1.00 11.20 N
778 CA GLU A of atom, 102 2.696 14.706 25.269 1.00 11.26 C
779 C GLU A of atom, 102 1.638 13.902 24.520 1.00 11.09 C
780 O GLU A of atom, 102 0.986 13.028 25.103 1.00 12.09 O
781 CB GLU A of atom, 102 2.169 16.085 25.643 1.00 11.82 C
782 CG GLU A of atom, 102 0.876 16.058 26.446 1.00 13.61 C
783 CD GLU A of atom, 102 1.012 15.407 27.817 1.00 17.38 C
784 OE1 GLU A of atom, 102 2.147 15.276 28.319 1.00 21.05 O
785 OE2 GLU A of atom, 102-0.027 15.024 28.399 1.00 16.09 O
786 N LEU A of atom, 103 1.437 14.194 23.231 1.00 10.13 N
787 CA LEU A of atom, 103 0.494 13.408 22.439 1.00 9.90 C
788 C LEU A of atom, 103 0.915 11.945 22.379 1.00 11.38 C
789 O LEU A of atom, 103 0.078 11.045 22.519 1.00 11.96 O
790 CB LEU A of atom, 103 0.372 14.001 21.034 1.00 10.61 C
791 CG LEU A of atom, 103-0.292 15.381 21.010 1.00 9.94 C
792 CD1 LEU A of atom, 103-0.007 16.112 19.699 1.00 10.34 C
793 CD2 LEU A of atom, 103-1.785 15.274 21.245 1.00 10.39 C
794 N GLN A of atom, 104 2.211 11.689 22.181 1.00 11.66 N
795 CA GLN A of atom, 104 2.706 10.317 22.115 1.00 15.01 C
796 C GLN A of atom, 104 2.522 9.601 23.445 1.00 16.30 C
797 O GLN A of atom, 104 2.176 8.410 23.477 1.00 16.61 O
798 CB GLN A of atom, 104 4.182 10.341 21.734 1.00 19.40 C
799 CG GLN A of atom, 104 4.709 9.060 21.120 1.00 24.50 C
800 CD GLN A of atom, 104 6.109 9.235 20.576 1.00 27.42 C
801 OE1 GLN A of atom, 104 7.008 9.672 21.293 1.00 29.60 O
802 NE2 GLN A of atom, 104 6.301 8.905 19.304 1.00 29.23 N
803 N AARG A of atom, 105 2.743 10.319 24.554 0.46 14.14 N
804 CA AARG A of atom, 105 2.556 9.767 25.894 0.46 15.11 C
805 C AARG A of atom, 105 1.131 9.273 26.103 0.46 14.62 C
806 O AARG A of atom, 105 0.903 8.331 26.874 0.46 16.95 O
807 CB AARG A of atom, 105 2.877 10.858 26.922 0.46 16.60 C
808 CG AARG A of atom, 105 3.452 10.382 28.245 0.46 19.73 C
809 CD AARG A of atom, 105 4.536 11.335 28.762 0.46 17.82 C
810 NE AARG A of atom, 105 4.195 12.752 28.614 0.46 15.19 N
811 CZ AARG A of atom, 105 5.070 13.695 28.266 0.46 15.08 C
812 NH1 AARG A of atom, 105 6.335 13.372 28.033 0.46 16.44 N
813 NH2 AARG A of atom, 105 4.686 14.959 28.156 0.46 10.44 N
814 N BARG A of atom, 105 2.778 10.293 24.556 0.54 14.12 N
815 CA BARG A of atom, 105 2.551 9.681 25.858 0.54 15.02 C
816 C BARG A of atom, 105 1.132 9.146 25.956 0.54 13.75 C
817 O BARG A of atom, 105 0.907 8.041 26.467 0.54 14.24 O
818 CB BARG A of atom, 105 2.812 10.704 26.964 0.54 16.06 C
819 CG BARG A of atom, 105 2.790 10.140 28.369 0.54 17.91 C
820 CD BARG A of atom, 105 3.034 11.248 29.382 0.54 15.61 C
821 NE BARG A of atom, 105 1.900 12.164 29.463 0.54 17.10 N
822 CZ BARG A of atom, 105 0.791 11.919 30.151 0.54 18.36 C
823 NH1 BARG A of atom, 105 0.667 10.786 30.830 0.54 20.13 N
824 NH2 BARG A of atom, 105-0.194 12.805 30.165 0.54 17.39 N
825 N ARG A of atom, 106 0.162 9.907 25.447 1.00 12.63 N
826 CA ARG A of atom, 106-1.239 9.525 25.513 1.00 12.23 C
827 C ARG A of atom, 106-1.655 8.626 24.365 1.00 12.43 C
828 O ARG A of atom, 106-2.843 8.320 24.241 1.00 12.72 O
829 CB ARG A of atom, 106-2.132 10.767 25.544 1.00 12.61 C
830 CG ARG A of atom, 106-1.768 11.727 26.664 1.00 14.30 C
831 CD ARG A of atom, 106-2.869 12.733 26.857 1.00 17.57 C
832 NE ARG A of atom, 106-2.466 13.794 27.767 1.00 14.22 N
833 CZ ARG A of atom, 106-3.301 14.457 28.554 1.00 12.21 C
834 NH1 ARG A of atom, 106-4.591 14.167 28.560 1.00 14.39 N
835 NH2 ARG A of atom, 106-2.832 15.401 29.347 1.00 13.95 N
836 N LYS A of atom, 107-0.709 8.218 23.522 1.00 12.23 N
837 CA LYS A of atom, 107-0.981 7.333 22.392 1.00 13.11 C
838 C LYS A of atom, 107-2.003 7.926 21.421 1.00 12.26 C
839 O LYS A of atom, 107-2.853 7.218 20.877 1.00 14.87 O
840 CB LYS A of atom, 107-1.363 5.926 22.855 1.00 16.42 C
841 CG LYS A of atom, 107-0.285 5.304 23.731 1.00 19.72 C
842 CD LYS A of atom, 107-0.460 3.810 23.905 1.00 28.91 C
843 CE LYS A of atom, 107 0.818 3.178 24.435 1.00 34.97 C
844 NZ LYS A of atom, 107 1.060 1.837 23.831 1.00 47.46 N
845 N ILE A of atom, 108-1.931 9.235 21.205 1.00 11.31 N
846 CA ILE A of atom, 108-2.783 9.930 20.243 1.00 10.76 C
847 C ILE A of atom, 108-2.013 10.060 18.934 1.00 10.62 C
848 O ILE A of atom, 108-0.937 10.684 18.921 1.00 11.18 O
849 CB ILE A of atom, 108-3.224 11.302 20.764 1.00 9.91 C
850 CG1 ILE A of atom, 108-4.076 11.123 22.021 1.00 10.88 C
851 CG2 ILE A of atom, 108-4.002 12.051 19.673 1.00 10.73 C
852 CD1 ILE A of atom, 108-4.349 12.407 22.769 1.00 11.68 C
853 N PRO A of atom, 109-2.501 9.481 17.837 1.00 10.45 N
854 CA PRO A of atom, 109-1.825 9.643 16.548 1.00 11.39 C
855 C PRO A of atom, 109-1.692 11.106 16.158 1.00 9.44 C
856 O PRO A of atom, 109-2.553 11.934 16.460 1.00 10.00 O
857 CB PRO A of atom, 109-2.746 8.895 15.577 1.00 12.79 C
858 CG PRO A of atom, 109-3.482 7.896 16.429 1.00 14.09 C
859 CD PRO A of atom, 109-3.690 8.612 17.739 1.00 11.42 C
860 N ILE A of atom, 110-0.625 11.403 15.418 1.00 10.57 N
861 CA ILE A of atom, 110-0.242 12.773 15.091 1.00 11.18 C
862 C ILE A of atom, 110-0.175 12.948 13.577 1.00 9.33 C
863 O ILE A of atom, 110 0.459 12.147 12.872 1.00 10.76 O
864 CB ILE A of atom, 110 1.136 13.110 15.679 1.00 9.51 C
865 CG1 ILE A of atom, 110 1.140 12.946 17.201 1.00 12.23 C
866 CG2 ILE A of atom, 110 1.566 14.523 15.290 1.00 11.51 C
867 CD1 ILE A of atom, 110 2.534 12.869 17.778 1.00 12.28 C
868 N LEU A of atom, 111-0.770 14.033 13.092 1.00 9.31 N
869 CA LEU A of atom, 111-0.502 14.569 11.763 1.00 9.40 C
870 C LEU A of atom, 111 0.100 15.959 11.946 1.00 8.05 C
871 O LEU A of atom, 111-0.523 16.838 12.552 1.00 9.93 O
872 CB LEU A of atom, 111-1.785 14.654 10.934 1.00 9.88 C
873 CG LEU A of atom, 111-1.649 15.310 9.561 1.00 10.44 C
874 CD1 LEU A of atom, 111-0.680 14.544 8.681 1.00 14.31 C
875 CD2 LEU A of atom, 111-3.012 15.403 8.893 1.00 12.06 C
876 N LEU A of atom, 112 1.300 16.163 11.421 1.00 8.59 N
877 CA LEU A of atom, 112 2.011 17.426 11.549 1.00 8.72 C
878 C LEU A of atom, 112 1.847 18.230 10.265 1.00 8.78 C
879 O LEU A of atom, 112 2.106 17.718 9.171 1.00 10.93 O
880 CB LEU A of atom, 112 3.491 17.132 11.782 1.00 12.12 C
881 CG LEU A of atom, 112 4.384 18.285 12.234 1.00 11.22 C
882 CD1 LEU A of atom, 112 3.936 18.867 13.564 1.00 11.69 C
883 CD2 LEU A of atom, 112 5.832 17.827 12.313 1.00 11.99 C
884 N MET A of atom, 113 1.420 19.481 10.392 1.00 9.38 N
885 CA MET A of atom, 113 1.292 20.393 9.257 1.00 9.26 C
886 C MET A of atom, 113 2.590 21.180 9.097 1.00 9.15 C
887 O MET A of atom, 113 2.946 21.996 9.961 1.00 9.68 O
888 CB MET A of atom, 113 0.116 21.336 9.470 1.00 9.89 C
889 CG MET A of atom, 113-1.241 20.646 9.499 1.00 9.90 C
890 SD MET A of atom, 113-1.488 19.596 8.063 1.00 12.33 S
891 CE MET A of atom, 113-3.270 19.529 7.977 1.00 12.46 C
892 N GLY A of atom, 114 3.311 20.919 8.010 1.00 9.15 N
893 CA GLY A of atom, 114 4.599 21.561 7.795 1.00 8.98 C
894 C GLY A of atom, 114 4.444 23.031 7.449 1.00 8.24 C
895 O GLY A of atom, 114 3.619 23.406 6.606 1.00 10.54 O
896 N MET A of atom, 115 5.239 23.874 8.105 1.00 9.94 N
897 CA MET A of atom, 115 5.290 25.302 7.823 1.00 10.11 C
898 C MET A of atom, 115 6.572 25.623 7.064 1.00 10.22 C
899 O MET A of atom, 115 7.578 24.913 7.181 1.00 10.24 O
900 CB MET A of atom, 115 5.216 26.125 9.112 1.00 10.22 C
901 CG MET A of atom, 115 4.034 25.749 10.012 1.00 10.41 C
902 SD MET A of atom, 115 2.414 25.883 9.231 1.00 13.47 S
903 CE MET A of atom, 115 2.382 27.618 8.831 1.00 18.39 C
904 N ARG A of atom, 116 6.530 26.689 6.269 1.00 10.34 N
905 CA ARG A of atom, 116 7.666 27.082 5.446 1.00 11.43 C
906 C ARG A of atom, 116 7.984 28.553 5.652 1.00 12.19 C
907 O ARG A of atom, 116 7.080 29.388 5.778 1.00 14.42 O
908 CB ARG A of atom, 116 7.390 26.828 3.966 1.00 12.51 C
909 CG ARG A of atom, 116 7.267 25.358 3.636 1.00 12.76 C
910 CD ARG A of atom, 116 8.621 24.672 3.669 1.00 12.47 C
911 NE ARG A of atom, 116 8.523 23.239 3.395 1.00 12.37 N
912 CZ ARG A of atom, 116 8.329 22.712 2.190 1.00 12.68 C
913 NH1 ARG A of atom, 116 8.225 23.497 1.123 1.00 15.78 N
914 NH2 ARG A of atom, 116 8.253 21.395 2.062 1.00 14.05 N
915 N ALA A of atom, 117 9.274 28.860 5.691 1.00 12.61 N
916 CA ALA A of atom, 117 9.675 30.235 5.954 1.00 13.36 C
917 C ALA A of atom, 117 9.718 31.025 4.650 1.00 16.59 C
918 O ALA A of atom, 117 10.152 30.499 3.624 1.00 14.36 O
919 CB ALA A of atom, 117 11.063 30.263 6.587 1.00 14.84 C
920 N PRO A of atom, 118 9.281 32.282 4.672 1.00 18.20 N
921 CA PRO A of atom, 118 9.443 33.145 3.506 1.00 18.07 C
922 C PRO A of atom, 118 10.880 33.626 3.419 1.00 19.39 C
923 O PRO A of atom, 118 11.636 33.529 4.395 1.00 18.97 O
924 CB PRO A of atom, 118 8.485 34.307 3.809 1.00 22.56 C
925 CG PRO A of atom, 118 8.522 34.415 5.282 1.00 25.38 C
926 CD PRO A of atom, 118 8.669 32.997 5.806 1.00 20.44 C
927 N PRO A of atom, 119 11.302 34.146 2.267 1.00 16.33 N
928 CA PRO A of atom, 119 12.726 34.479 2.086 1.00 16.75 C
929 C PRO A of atom, 119 13.223 35.673 2.889 1.00 20.20 C
930 O PRO A of atom, 119 14.442 35.876 2.940 1.00 16.16 O
931 CB PRO A of atom, 119 12.834 34.749 0.580 1.00 19.41 C
932 CG PRO A of atom, 119 11.441 35.130 0.171 1.00 23.38 C
933 CD PRO A of atom, 119 10.541 34.285 1.014 1.00 22.66 C
934 N AASN A of atom, 120 12.343 36.492 3.473 0.45 18.06 N
935 CA AASN A of atom, 120 12.820 37.711 4.121 0.45 17.98 C
936 C AASN A of atom, 120 13.655 37.440 5.363 0.45 17.95 C
937 O AASN A of atom, 120 14.328 38.355 5.850 0.45 17.64 O
938 CB AASN A of atom, 120 11.675 38.689 4.418 0.45 21.66 C
939 CG AASN A of atom, 120 10.627 38.111 5.347 0.45 22.16 C
940 OD1 AASN A of atom, 120 10.925 37.712 6.473 0.45 25.19 O
941 ND2 AASN A of atom, 120 9.382 38.084 4.886 0.45 22.32 N
942 N BASN A of atom, 120 12.336 36.406 3.573 0.55 17.77 N
943 CA BASN A of atom, 120 12.717 37.671 4.205 0.55 17.66 C
944 C BASN A of atom, 120 13.824 37.496 5.241 0.55 17.54 C
945 O BASN A of atom, 120 14.739 38.323 5.320 0.55 19.53 O
946 CB BASN A of atom, 120 11.497 38.340 4.837 0.55 20.26 C
947 CG BASN A of atom, 120 11.780 39.763 5.282 0.55 23.12 C
948 OD1 BASN A of atom, 120 12.268 40.586 4.504 0.55 22.84 O
949 ND2 BASN A of atom, 120 11.475 40.060 6.540 0.55 20.57 N
950 N ALEU A of atom, 121 13.640 36.217 5.881 0.45 18.00 N
951 CA ALEU A of atom, 121 14.499 35.867 6.998 0.45 18.88 C
952 C ALEU A of atom, 121 15.847 35.310 6.559 0.45 14.41 C
953 O ALEU A of atom, 121 16.652 34.951 7.422 0.45 13.19 O
954 CB ALEU A of atom, 121 13.776 34.915 7.962 0.45 19.21 C
955 CG ALEU A of atom, 121 12.811 35.680 8.880 0.45 21.26 C
956 CD1 ALEU A of atom, 121 12.303 34.834 10.024 0.45 18.21 C
957 CD2 ALEU A of atom, 121 13.475 36.939 9.417 0.45 19.40 C
958 N BLEU A of atom, 121 13.757 36.442 6.056 0.55 17.77 N
959 CA BLEU A of atom, 121 14.788 36.258 7.069 0.55 19.53 C
960 C BLEU A of atom, 121 16.090 35.703 6.509 0.55 15.53 C
961 O BLEU A of atom, 121 17.083 35.664 7.243 0.55 16.85 O
962 CB BLEU A of atom, 121 14.302 35.357 8.208 0.55 19.57 C
963 CG BLEU A of atom, 121 12.952 35.678 8.840 0.55 21.55 C
964 CD1 BLEU A of atom, 121 11.920 34.682 8.356 0.55 19.71 C
965 CD2 BLEU A of atom, 121 13.069 35.640 10.346 0.55 18.34 C
966 N GLY A of atom, 122 16.113 35.273 5.255 1.00 13.79 N
967 CA GLY A of atom, 122 17.355 34.799 4.676 1.00 14.03 C
968 C GLY A of atom, 122 17.399 33.284 4.561 1.00 11.39 C
969 O GLY A of atom, 122 16.690 32.553 5.255 1.00 11.72 O
970 N ALA A of atom, 123 18.280 32.810 3.673 1.00 10.65 N
971 CA ALA A of atom, 123 18.359 31.382 3.374 1.00 10.25 C
972 C ALA A of atom, 123 18.820 30.564 4.573 1.00 10.42 C
973 O ALA A of atom, 123 18.357 29.436 4.773 1.00 10.52 O
974 CB ALA A of atom, 123 19.294 31.142 2.189 1.00 11.93 C
975 N LYS A of atom, 124 19.760 31.086 5.360 1.00 9.27 N
976 CA LYS A of atom, 124 20.242 30.335 6.518 1.00 9.73 C
977 C LYS A of atom, 124 19.107 30.043 7.494 1.00 9.97 C
978 O LYS A of atom, 124 18.900 28.895 7.896 1.00 10.78 O
979 CB LYS A of atom, 124 21.374 31.096 7.200 1.00 10.90 C
980 CG LYS A of atom, 124 21.941 30.365 8.395 1.00 12.19 C
981 CD LYS A of atom, 124 23.173 31.084 8.935 1.00 14.79 C
982 CE LYS A of atom, 124 23.728 30.420 10.194 1.00 17.28 C
983 NZ LYS A of atom, 124 25.012 31.072 10.614 1.00 21.92 N
984 N TYR A of atom, 125 18.351 31.074 7.871 1.00 9.44 N
985 CA TYR A of atom, 125 17.202 30.878 8.748 1.00 9.65 C
986 C TYR A of atom, 125 16.156 29.989 8.082 1.00 8.12 C
987 O TYR A of atom, 125 15.623 29.063 8.702 1.00 10.17 O
988 CB TYR A of atom, 125 16.600 32.235 9.120 1.00 10.63 C
989 CG TYR A of atom, 125 15.446 32.165 10.090 1.00 9.90 C
990 CD1 TYR A of atom, 125 14.177 31.738 9.695 1.00 12.25 C
991 CD2 TYR A of atom, 125 15.628 32.527 11.406 1.00 10.38 C
992 CE1 TYR A of atom, 125 13.138 31.665 10.607 1.00 12.54 C
993 CE2 TYR A of atom, 125 14.595 32.467 12.319 1.00 10.62 C
994 CZ TYR A of atom, 125 13.351 32.031 11.911 1.00 9.95 C
995 OH TYR A of atom, 125 12.284 31.972 12.782 1.00 10.99 O
996 N GLN A of atom, 126 15.836 30.270 6.820 1.00 9.41 N
997 CA GLN A of atom, 126 14.759 29.545 6.151 1.00 10.22 C
998 C GLN A of atom, 126 15.052 28.049 6.065 1.00 10.11 C
999 O GLN A of atom, 126 14.178 27.217 6.342 1.00 9.95 O
1000 CB GLN A of atom, 126 14.492 30.157 4.777 1.00 10.46 C
1001 CG GLN A of atom, 126 13.733 29.258 3.826 1.00 11.57 C
1002 CD GLN A of atom, 126 14.662 28.413 2.988 1.00 13.05 C
1003 OE1 GLN A of atom, 126 15.691 28.895 2.506 1.00 13.10 O
1004 NE2 GLN A of atom, 126 14.314 27.145 2.812 1.00 13.07 N
1005 N ARG A of atom, 127 16.274 27.682 5.682 1.00 9.51 N
1006 CA ARG A of atom, 127 16.606 26.265 5.619 1.00 10.45 C
1007 C ARG A of atom, 127 16.493 25.623 6.990 1.00 9.80 C
1008 O ARG A of atom, 127 15.999 24.497 7.121 1.00 10.64 O
1009 CB ARG A of atom, 127 18.028 26.076 5.107 1.00 12.33 C
1010 CG ARG A of atom, 127 18.183 26.265 3.601 1.00 14.70 C
1011 CD ARG A of atom, 127 19.618 25.981 3.153 1.00 18.99 C
1012 NE ARG A of atom, 127 20.572 26.904 3.759 1.00 16.32 N
1013 CZ ARG A of atom, 127 21.348 27.737 3.077 1.00 15.10 C
1014 NH1 ARG A of atom, 127 21.281 27.772 1.752 1.00 20.51 N
1015 NH2 ARG A of atom, 127 22.181 28.542 3.722 1.00 17.68 N
1016 N GLU A of atom, 128 16.984 26.310 8.024 1.00 9.52 N
1017 CA GLU A of atom, 128 16.920 25.755 9.370 1.00 9.50 C
1018 C GLU A of atom, 128 15.476 25.569 9.818 1.00 8.85 C
1019 O GLU A of atom, 128 15.110 24.512 10.347 1.00 10.55 O
1020 CB GLU A of atom, 128 17.685 26.645 10.354 1.00 10.54 C
1021 CG GLU A of atom, 128 17.869 25.965 11.718 1.00 11.45 C
1022 CD GLU A of atom, 128 18.446 26.845 12.812 1.00 12.19 C
1023 OE1 GLU A of atom, 128 18.853 27.993 12.557 1.00 12.56 O
1024 OE2 GLU A of atom, 128 18.470 26.369 13.967 1.00 13.07 O
1025 N APHE A of atom, 129 14.648 26.599 9.623 0.65 9.02 N
1026 CA APHE A of atom, 129 13.252 26.510 10.027 0.65 9.27 C
1027 C APHE A of atom, 129 12.534 25.394 9.281 0.65 8.98 C
1028 O APHE A of atom, 129 11.844 24.565 9.886 0.65 9.91 O
1029 CB APHE A of atom, 129 12.545 27.841 9.776 0.65 10.21 C
1030 CG APHE A of atom, 129 11.096 27.797 10.128 0.65 10.35 C
1031 CD1 APHE A of atom, 129 10.692 27.983 11.444 0.65 12.70 C
1032 CD2 APHE A of atom, 129 10.134 27.507 9.164 0.65 12.20 C
1033 CE1 APHE A of atom, 129 9.351 27.912 11.789 0.65 13.77 C
1034 CE2 APHE A of atom, 129 8.792 27.428 9.503 0.65 12.37 C
1035 CZ APHE A of atom, 129 8.400 27.629 10.819 0.65 13.41 C
1036 N BPHE A of atom, 129 14.631 26.580 9.606 0.35 9.21 N
1037 CA BPHE A of atom, 129 13.255 26.462 10.078 0.35 9.39 C
1038 C BPHE A of atom, 129 12.469 25.427 9.277 0.35 9.18 C
1039 O BPHE A of atom, 129 11.692 24.658 9.852 0.35 9.82 O
1040 CB BPHE A of atom, 129 12.552 27.823 10.110 0.35 11.75 C
1041 CG BPHE A of atom, 129 11.517 27.931 11.203 0.35 11.37 C
1042 CD1 BPHE A of atom, 129 10.356 27.174 11.149 0.35 12.15 C
1043 CD2 BPHE A of atom, 129 11.716 28.761 12.299 0.35 10.61 C
1044 CE1 BPHE A of atom, 129 9.413 27.250 12.155 0.35 12.80 C
1045 CE2 BPHE A of atom, 129 10.772 28.841 13.308 0.35 11.00 C
1046 CZ BPHE A of atom, 129 9.621 28.086 13.234 0.35 11.38 C
1047 N ASP A of atom, 130 12.656 25.385 7.953 1.00 9.67 N
1048 CA ASP A of atom, 130 11.893 24.433 7.152 1.00 9.31 C
1049 C ASP A of atom, 130 12.211 23.003 7.558 1.00 9.64 C
1050 O ASP A of atom, 130 11.333 22.130 7.533 1.00 10.63 O
1051 CB ASP A of atom, 130 12.203 24.622 5.670 1.00 10.66 C
1052 CG ASP A of atom, 130 11.599 25.872 5.094 1.00 10.23 C
1053 OD1 ASP A of atom, 130 10.945 26.650 5.813 1.00 11.45 O
1054 OD2 ASP A of atom, 130 11.804 26.069 3.882 1.00 11.02 O
1055 N GLY A of atom, 131 13.461 22.741 7.944 1.00 9.42 N
1056 CA GLY A of atom, 131 13.869 21.377 8.249 1.00 9.90 C
1057 C GLY A of atom, 131 13.344 20.830 9.564 1.00 8.99 C
1058 O GLY A of atom, 131 13.461 19.623 9.805 1.00 10.30 O
1059 N ILE A of atom, 132 12.785 21.674 10.431 1.00 9.01 N
1060 CA ILE A of atom, 132 12.351 21.135 11.721 1.00 8.91 C
1061 C ILE A of atom, 132 11.181 20.177 11.554 1.00 8.61 C
1062 O ILE A of atom, 132 11.043 19.226 12.329 1.00 9.50 O
1063 CB ILE A of atom, 132 12.076 22.214 12.791 1.00 10.64 C
1064 CG1 ILE A of atom, 132 10.761 22.964 12.545 1.00 9.98 C
1065 CG2 ILE A of atom, 132 13.265 23.162 12.922 1.00 11.44 C
1066 CD1 ILE A of atom, 132 10.399 23.971 13.644 1.00 11.88 C
1067 N TYR A of atom, 133 10.338 20.390 10.535 1.00 9.38 N
1068 CA TYR A of atom, 133 9.132 19.573 10.410 1.00 8.99 C
1069 C TYR A of atom, 133 9.432 18.147 9.969 1.00 9.72 C
1070 O TYR A of atom, 133 8.939 17.205 10.617 1.00 10.00 O
1071 CB TYR A of atom, 133 8.081 20.292 9.552 1.00 10.09 C
1072 CG TYR A of atom, 133 7.637 21.574 10.221 1.00 8.61 C
1073 CD1 TYR A of atom, 133 6.636 21.567 11.187 1.00 8.73 C
1074 CD2 TYR A of atom, 133 8.260 22.784 9.933 1.00 9.50 C
1075 CE1 TYR A of atom, 133 6.261 22.732 11.848 1.00 9.36 C
1076 CE2 TYR A of atom, 133 7.891 23.949 10.574 1.00 9.41 C
1077 CZ TYR A of atom, 133 6.893 23.915 11.533 1.00 6.97 C
1078 OH TYR A of atom, 133 6.553 25.084 12.165 1.00 9.21 O
1079 N PRO A of atom, 134 10.223 17.903 8.918 1.00 10.31 N
1080 CA PRO A of atom, 134 10.577 16.508 8.614 1.00 11.10 C
1081 C PRO A of atom, 134 11.335 15.837 9.741 1.00 12.30 C
1082 O PRO A of atom, 134 11.147 14.636 9.983 1.00 12.88 O
1083 CB PRO A of atom, 134 11.417 16.606 7.331 1.00 12.71 C
1084 CG PRO A of atom, 134 11.089 17.934 6.744 1.00 17.29 C
1085 CD PRO A of atom, 134 10.721 18.835 7.888 1.00 10.25 C
1086 N TYR A of atom, 135 12.166 16.587 10.464 1.00 11.35 N
1087 CA TYR A of atom, 135 12.869 16.017 11.605 1.00 11.09 C
1088 C TYR A of atom, 135 11.878 15.539 12.662 1.00 11.97 C
1089 O TYR A of atom, 135 11.992 14.419 13.183 1.00 12.88 O
1090 CB TYR A of atom, 135 13.823 17.053 12.198 1.00 13.77 C
1091 CG TYR A of atom, 135 14.436 16.611 13.502 1.00 16.42 C
1092 CD1 TYR A of atom, 135 15.595 15.843 13.520 1.00 20.53 C
1093 CD2 TYR A of atom, 135 13.862 16.962 14.714 1.00 16.18 C
1094 CE1 TYR A of atom, 135 16.160 15.431 14.710 1.00 23.68 C
1095 CE2 TYR A of atom, 135 14.422 16.551 15.917 1.00 20.34 C
1096 CZ TYR A of atom, 135 15.569 15.787 15.899 1.00 21.31 C
1097 OH TYR A of atom, 135 16.129 15.378 17.086 1.00 28.56 O
1098 N LEU A of atom, 136 10.906 16.387 13.010 1.00 10.06 N
1099 CA LEU A of atom, 136 9.940 16.026 14.047 1.00 11.32 C
1100 C LEU A of atom, 136 9.062 14.861 13.616 1.00 10.52 C
1101 O LEU A of atom, 136 8.734 13.992 14.434 1.00 12.06 O
1102 CB LEU A of atom, 136 9.059 17.229 14.370 1.00 12.27 C
1103 CG LEU A of atom, 136 9.742 18.388 15.084 1.00 12.04 C
1104 CD1 LEU A of atom, 136 8.934 19.670 14.891 1.00 13.51 C
1105 CD2 LEU A of atom, 136 9.930 18.063 16.566 1.00 17.38 C
1106 N ALA A of atom, 137 8.626 14.851 12.353 1.00 11.12 N
1107 CA ALA A of atom, 137 7.772 13.765 11.889 1.00 12.57 C
1108 C ALA A of atom, 137 8.488 12.429 11.997 1.00 12.90 C
1109 O ALA A of atom, 137 7.896 11.432 12.428 1.00 14.69 O
1110 CB ALA A of atom, 137 7.327 14.031 10.454 1.00 14.44 C
1111 N AGLU A of atom, 138 9.777 12.406 11.646 0.41 13.54 N
1112 CA AGLU A of atom, 138 10.569 11.181 11.702 0.41 16.43 C
1113 C AGLU A of atom, 138 10.826 10.757 13.142 0.41 16.84 C
1114 O AGLU A of atom, 138 10.779 9.564 13.465 0.41 18.77 O
1115 CB AGLU A of atom, 138 11.891 11.418 10.971 0.41 17.69 C
1116 CG AGLU A of atom, 138 12.871 10.258 10.989 0.41 21.55 C
1117 CD AGLU A of atom, 138 13.726 10.215 9.735 0.41 25.05 C
1118 OE1 AGLU A of atom, 138 13.469 9.352 8.870 0.41 30.93 O
1119 OE2 AGLU A of atom, 138 14.648 11.049 9.609 0.41 29.83 O
1120 N BGLU A of atom, 138 9.762 12.379 11.608 0.59 13.58 N
1121 CA BGLU A of atom, 138 10.508 11.132 11.737 0.59 16.37 C
1122 C BGLU A of atom, 138 10.677 10.760 13.201 0.59 16.57 C
1123 O BGLU A of atom, 138 10.421 9.617 13.599 0.59 16.61 O
1124 CB BGLU A of atom, 138 11.875 11.258 11.064 0.59 17.38 C
1125 CG BGLU A of atom, 138 12.550 9.915 10.766 0.59 21.62 C
1126 CD BGLU A of atom, 138 13.290 9.333 11.963 0.59 23.95 C
1127 OE1 BGLU A of atom, 138 13.597 10.087 12.912 0.59 25.98 O
1128 OE2 BGLU A of atom, 138 13.572 8.113 11.950 0.59 27.40 O
1129 N LYS A of atom, 139 11.095 11.720 14.023 1.00 15.11 N
1130 CA LYS A of atom, 139 11.432 11.393 15.401 1.00 15.82 C
1131 C LYS A of atom, 139 10.223 10.877 16.165 1.00 16.69 C
1132 O LYS A of atom, 139 10.341 9.949 16.976 1.00 17.18 O
1133 CB LYS A of atom, 139 12.025 12.621 16.088 1.00 16.52 C
1134 CG LYS A of atom, 139 12.413 12.371 17.530 1.00 15.19 C
1135 CD LYS A of atom, 139 13.179 13.538 18.123 1.00 19.86 C
1136 CE LYS A of atom, 139 13.399 13.341 19.621 1.00 22.25 C
1137 NZ LYS A of atom, 139 14.277 14.404 20.179 1.00 29.14 N
1138 N TYR A of atom, 140 9.047 11.446 15.911 1.00 13.85 N
1139 CA TYR A of atom, 140 7.856 11.127 16.682 1.00 15.64 C
1140 C TYR A of atom, 140 6.877 10.237 15.932 1.00 16.63 C
1141 O TYR A of atom, 140 5.735 10.085 16.377 1.00 17.69 O
1142 CB TYR A of atom, 140 7.198 12.407 17.208 1.00 16.90 C
1143 CG TYR A of atom, 140 8.096 13.081 18.213 1.00 15.04 C
1144 CD1 TYR A of atom, 140 8.223 12.573 19.493 1.00 17.92 C
1145 CD2 TYR A of atom, 140 8.877 14.169 17.860 1.00 14.61 C
1146 CE1 TYR A of atom, 140 9.075 13.155 20.407 1.00 17.89 C
1147 CE2 TYR A of atom, 140 9.731 14.761 18.767 1.00 15.54 C
1148 CZ TYR A of atom, 140 9.825 14.251 20.049 1.00 16.72 C
1149 OH TYR A of atom, 140 10.682 14.838 20.956 1.00 18.82 O
1150 N ASP A of atom, 141 7.307 9.632 14.824 1.00 17.02 N
1151 CA ASP A of atom, 141 6.469 8.735 14.028 1.00 19.04 C
1152 C ASP A of atom, 141 5.129 9.389 13.697 1.00 21.25 C
1153 O ASP A of atom, 141 4.052 8.808 13.861 1.00 21.49 O
1154 CB ASP A of atom, 141 6.294 7.375 14.707 1.00 24.94 C
1155 CG ASP A of atom, 141 5.526 6.385 13.846 1.00 33.21 C
1156 OD1 ASP A of atom, 141 5.569 6.514 12.603 1.00 37.35 O
1157 OD2 ASP A of atom, 141 4.868 5.490 14.418 1.00 41.81 O
1158 N ALA A of atom, 142 5.206 10.631 13.240 1.00 16.03 N
1159 CA ALA A of atom, 142 4.031 11.393 12.871 1.00 15.70 C
1160 C ALA A of atom, 142 3.939 11.449 11.358 1.00 13.41 C
1161 O ALA A of atom, 142 4.957 11.508 10.662 1.00 15.87 O
1162 CB ALA A of atom, 142 4.118 12.817 13.419 1.00 16.11 C
1163 N LYS A of atom, 143 2.722 11.430 10.848 1.00 12.61 N
1164 CA LYS A of atom, 143 2.542 11.729 9.439 1.00 13.06 C
1165 C LYS A of atom, 143 2.743 13.222 9.224 1.00 11.61 C
1166 O LYS A of atom, 143 2.542 14.030 10.136 1.00 12.79 O
1167 CB LYS A of atom, 143 1.163 11.282 8.984 1.00 14.70 C
1168 CG LYS A of atom, 143 1.073 9.759 8.851 1.00 22.53 C
1169 CD LYS A of atom, 143-0.353 9.277 8.728 1.00 30.17 C
1170 CE LYS A of atom, 143-0.400 7.816 8.295 1.00 33.58 C
1171 NZ LYS A of atom, 143-0.563 6.898 9.456 1.00 34.04 N
1172 N LEU A of atom, 144 3.175 13.585 8.021 1.00 12.27 N
1173 CA LEU A of atom, 144 3.557 14.959 7.723 1.00 11.86 C
1174 C LEU A of atom, 144 2.888 15.403 6.433 1.00 11.68 C
1175 O LEU A of atom, 144 2.968 14.709 5.412 1.00 12.65 O
1176 CB LEU A of atom, 144 5.075 15.080 7.558 1.00 13.11 C
1177 CG LEU A of atom, 144 5.590 16.441 7.087 1.00 11.86 C
1178 CD1 LEU A of atom, 144 5.395 17.504 8.162 1.00 12.57 C
1179 CD2 LEU A of atom, 144 7.055 16.340 6.705 1.00 16.97 C
1180 N VAL A of atom, 145 2.237 16.561 6.473 1.00 10.10 N
1181 CA VAL A of atom, 145 1.930 17.329 5.273 1.00 11.10 C
1182 C VAL A of atom, 145 3.104 18.277 5.061 1.00 10.39 C
1183 O VAL A of atom, 145 3.296 19.187 5.879 1.00 10.07 O
1184 CB VAL A of atom, 145 0.617 18.108 5.421 1.00 11.33 C
1185 CG1 VAL A of atom, 145 0.447 19.100 4.279 1.00 12.84 C
1186 CG2 VAL A of atom, 145-0.579 17.151 5.482 1.00 13.61 C
1187 N PRO A of atom, 146 3.940 18.070 4.044 1.00 10.26 N
1188 CA PRO A of atom, 146 5.200 18.824 3.996 1.00 10.89 C
1189 C PRO A of atom, 146 5.006 20.325 3.953 1.00 10.27 C
1190 O PRO A of atom, 146 5.771 21.062 4.585 1.00 10.24 O
1191 CB PRO A of atom, 146 5.887 18.292 2.729 1.00 13.22 C
1192 CG PRO A of atom, 146 5.327 16.916 2.565 1.00 16.38 C
1193 CD PRO A of atom, 146 3.888 17.008 3.024 1.00 12.89 C
1194 N PHE A of atom, 147 3.991 20.809 3.237 1.00 10.60 N
1195 CA PHE A of atom, 147 3.806 22.248 3.027 1.00 10.68 C
1196 C PHE A of atom, 147 2.306 22.519 3.125 1.00 9.65 C
1197 O PHE A of atom, 147 1.577 22.457 2.130 1.00 10.23 O
1198 CB PHE A of atom, 147 4.405 22.666 1.684 1.00 12.15 C
1199 CG PHE A of atom, 147 4.365 24.159 1.393 1.00 12.32 C
1200 CD1 PHE A of atom, 147 3.974 25.083 2.327 1.00 12.18 C
1201 CD2 PHE A of atom, 147 4.735 24.616 0.141 1.00 15.16 C
1202 CE1 PHE A of atom, 147 3.948 26.453 2.019 1.00 15.04 C
1203 CE2 PHE A of atom, 147 4.712 25.972-0.167 1.00 15.64 C
1204 CZ PHE A of atom, 147 4.317 26.886 0.765 1.00 17.13 C
1205 N PHE A of atom, 148 1.850 22.831 4.336 1.00 9.83 N
1206 CA PHE A of atom, 148 0.419 22.977 4.575 1.00 10.24 C
1207 C PHE A of atom, 148-0.204 24.034 3.671 1.00 10.84 C
1208 O PHE A of atom, 148-1.309 23.839 3.151 1.00 10.12 O
1209 CB PHE A of atom, 148 0.192 23.306 6.048 1.00 10.44 C
1210 CG PHE A of atom, 148-1.243 23.558 6.398 1.00 10.54 C
1211 CD1 PHE A of atom, 148-2.222 22.637 6.067 1.00 14.60 C
1212 CD2 PHE A of atom, 148-1.615 24.713 7.056 1.00 14.30 C
1213 CE1 PHE A of atom, 148-3.551 22.864 6.395 1.00 16.46 C
1214 CE2 PHE A of atom, 148-2.943 24.943 7.387 1.00 17.34 C
1215 CZ PHE A of atom, 148-3.907 24.014 7.049 1.00 16.59 C
1216 N LEU A of atom, 149 0.475 25.157 3.470 1.00 9.60 N
1217 CA LEU A of atom, 149-0.088 26.251 2.679 1.00 10.22 C
1218 C LEU A of atom, 149 0.236 26.155 1.190 1.00 10.27 C
1219 O LEU A of atom, 149 0.023 27.133 0.460 1.00 11.08 O
1220 CB LEU A of atom, 149 0.370 27.606 3.226 1.00 10.11 C
1221 CG LEU A of atom, 149-0.182 27.967 4.603 1.00 11.92 C
1222 CD1 LEU A of atom, 149 0.366 29.315 5.043 1.00 14.99 C
1223 CD2 LEU A of atom, 149-1.709 27.984 4.607 1.00 15.92 C
1224 N GLU A of atom, 150 0.748 25.020 0.723 1.00 10.00 N
1225 CA GLU A of atom, 150 1.138 24.895-0.679 1.00 12.27 C
1226 C GLU A of atom, 150 0.020 25.295-1.633 1.00 10.86 C
1227 O GLU A of atom, 150 0.272 25.948-2.653 1.00 12.01 O
1228 CB GLU A of atom, 150 1.572 23.463-0.956 1.00 11.46 C
1229 CG GLU A of atom, 150 2.131 23.249-2.348 1.00 14.66 C
1230 CD GLU A of atom, 150 2.689 21.847-2.513 1.00 25.34 C
1231 OE1 GLU A of atom, 150 2.058 20.893-2.007 1.00 30.31 O
1232 OE2 GLU A of atom, 150 3.755 21.699-3.147 1.00 34.85 O
1233 N ALA A of atom, 151-1.218 24.911-1.331 1.00 10.51 N
1234 CA ALA A of atom, 151-2.289 25.127-2.297 1.00 11.45 C
1235 C ALA A of atom, 151-2.663 26.592-2.460 1.00 12.64 C
1236 O ALA A of atom, 151-3.346 26.921-3.435 1.00 14.72 O
1237 CB ALA A of atom, 151-3.511 24.293-1.935 1.00 13.46 C
1238 N VAL A of atom, 152-2.241 27.481-1.567 1.00 11.90 N
1239 CA VAL A of atom, 152-2.456 28.911-1.765 1.00 13.11 C
1240 C VAL A of atom, 152-1.171 29.653-2.086 1.00 13.74 C
1241 O VAL A of atom, 152-1.207 30.880-2.277 1.00 14.66 O
1242 CB VAL A of atom, 152-3.213 29.561-0.593 1.00 12.93 C
1243 CG1 VAL A of atom, 152-4.600 28.940-0.455 1.00 16.42 C
1244 CG2 VAL A of atom, 152-2.411 29.438 0.706 1.00 14.95 C
1245 N ALA A of atom, 153-0.048 28.936-2.224 1.00 16.69 N
1246 CA ALA A of atom, 153 1.262 29.573-2.335 1.00 22.91 C
1247 C ALA A of atom, 153 1.354 30.460-3.566 1.00 22.50 C
1248 O ALA A of atom, 153 2.012 31.507-3.539 1.00 30.21 O
1249 CB ALA A of atom, 153 2.353 28.502-2.375 1.00 24.30 C
1250 N ASP A of atom, 154 0.720 30.049-4.660 1.00 19.76 N
1251 CA ASP A of atom, 154 0.766 30.794-5.909 1.00 21.53 C
1252 C ASP A of atom, 154-0.542 31.517-6.193 1.00 17.27 C
1253 O ASP A of atom, 154-0.833 31.830-7.351 1.00 18.88 O
1254 CB ASP A of atom, 154 1.122 29.867-7.073 1.00 23.91 C
1255 CG ASP A of atom, 154 2.500 29.256-6.931 1.00 40.79 C
1256 OD1 ASP A of atom, 154 3.426 29.970-6.491 1.00 43.68 O
1257 OD2 ASP A of atom, 154 2.656 28.059-7.259 1.00 51.68 O
1258 N ARG A of atom, 155-1.353 31.770-5.168 1.00 13.78 N
1259 CA ARG A of atom, 155-2.717 32.257-5.355 1.00 12.13 C
1260 C ARG A of atom, 155-2.979 33.453-4.451 1.00 13.31 C
1261 O ARG A of atom, 155-3.694 33.348-3.450 1.00 11.84 O
1262 CB ARG A of atom, 155-3.720 31.133-5.097 1.00 11.76 C
1263 CG ARG A of atom, 155-3.695 30.013-6.122 1.00 11.87 C
1264 CD ARG A of atom, 155-4.217 30.454-7.483 1.00 11.22 C
1265 NE ARG A of atom, 155-5.551 31.053-7.452 1.00 10.93 N
1266 CZ ARG A of atom, 155-6.688 30.388-7.628 1.00 10.40 C
1267 NH1 ARG A of atom, 155-6.676 29.076-7.812 1.00 12.13 N
1268 NH2 ARG A of atom, 155-7.846 31.034-7.620 1.00 10.93 N
1269 N PRO A of atom, 156-2.424 34.620-4.787 1.00 15.63 N
1270 CA PRO A of atom, 156-2.713 35.817-3.977 1.00 15.46 C
1271 C PRO A of atom, 156-4.189 36.160-3.918 1.00 14.67 C
1272 O PRO A of atom, 156-4.629 36.787-2.948 1.00 16.35 O
1273 CB PRO A of atom, 156-1.886 36.924-4.651 1.00 21.28 C
1274 CG PRO A of atom, 156-1.494 36.385-5.978 1.00 21.80 C
1275 CD PRO A of atom, 156-1.462 34.894-5.869 1.00 17.16 C
1276 N ASP A of atom, 157-4.975 35.737-4.909 1.00 12.29 N
1277 CA ASP A of atom, 157-6.416 35.950-4.876 1.00 13.35 C
1278 C ASP A of atom, 157-7.124 35.116-3.813 1.00 13.08 C
1279 O ASP A of atom, 157-8.303 35.370-3.535 1.00 13.70 O
1280 CB ASP A of atom, 157-7.034 35.683-6.248 1.00 12.70 C
1281 CG ASP A of atom, 157-6.724 34.296-6.787 1.00 12.73 C
1282 OD1 ASP A of atom, 157-5.655 33.726-6.454 1.00 13.07 O
1283 OD2 ASP A of atom, 157-7.534 33.784-7.590 1.00 13.59 O
1284 N LEU A of atom, 158-6.429 34.154-3.196 1.00 11.11 N
1285 CA LEU A of atom, 158-6.988 33.295-2.161 1.00 10.08 C
1286 C LEU A of atom, 158-6.438 33.629-0.787 1.00 12.24 C
1287 O LEU A of atom, 158-6.644 32.859 0.159 1.00 12.42 O
1288 CB LEU A of atom, 158-6.715 31.827-2.484 1.00 11.43 C
1289 CG LEU A of atom, 158-7.311 31.301-3.791 1.00 11.35 C
1290 CD1 LEU A of atom, 158-6.947 29.846-3.969 1.00 12.82 C
1291 CD2 LEU A of atom, 158-8.825 31.492-3.843 1.00 13.55 C
1292 N ILE A of atom, 159-5.748 34.761-0.658 1.00 12.74 N
1293 CA ILE A of atom, 159-5.130 35.197 0.588 1.00 14.92 C
1294 C ILE A of atom, 159-5.782 36.508 0.992 1.00 14.58 C
1295 O ILE A of atom, 159-5.947 37.409 0.158 1.00 14.30 O
1296 CB ILE A of atom, 159-3.608 35.367 0.422 1.00 17.20 C
1297 CG1 ILE A of atom, 159-2.967 34.040 0.002 1.00 17.99 C
1298 CG2 ILE A of atom, 159-2.974 35.894 1.697 1.00 18.69 C
1299 CD1 ILE A of atom, 159-3.298 32.908 0.918 1.00 22.39 C
1300 N GLN A of atom, 160-6.176 36.602 2.256 1.00 15.16 N
1301 CA GLN A of atom, 160-6.796 37.813 2.765 1.00 16.24 C
1302 C GLN A of atom, 160-5.799 38.958 2.726 1.00 19.09 C
1303 O GLN A of atom, 160-4.601 38.776 2.939 1.00 18.47 O
1304 CB GLN A of atom, 160-7.203 37.615 4.222 1.00 17.93 C
1305 CG GLN A of atom, 160-8.282 36.584 4.462 1.00 25.01 C
1306 CD GLN A of atom, 160-8.623 36.472 5.933 1.00 24.65 C
1307 OE1 GLN A of atom, 160-8.742 37.482 6.630 1.00 30.72 O
1308 NE2 GLN A of atom, 160-8.754 35.242 6.422 1.00 22.16 N
1309 N ALYS A of atom, 161-6.309 40.139 2.409 0.69 20.65 N
1310 CA ALYS A of atom, 161-5.564 41.375 2.573 0.69 23.23 C
1311 C ALYS A of atom, 161-5.808 41.845 4.000 0.69 25.37 C
1312 O ALYS A of atom, 161-6.941 42.176 4.365 0.69 24.88 O
1313 CB ALYS A of atom, 161-6.057 42.414 1.571 0.69 25.96 C
1314 CG ALYS A of atom, 161-5.343 43.754 1.650 0.69 26.87 C
1315 CD ALYS A of atom, 161-3.832 43.580 1.721 0.69 31.00 C
1316 CE ALYS A of atom, 161-3.132 44.272 0.558 0.69 28.07 C
1317 NZ ALYS A of atom, 161-2.883 43.333-0.572 0.69 30.55 N
1318 N BLYS A of atom, 161-6.288 40.161 2.470 0.31 21.09 N
1319 CA BLYS A of atom, 161-5.438 41.347 2.546 0.31 23.43 C
1320 C BLYS A of atom, 161-5.696 42.015 3.891 0.31 25.66 C
1321 O BLYS A of atom, 161-6.722 42.670 4.086 0.31 26.86 O
1322 CB BLYS A of atom, 161-5.698 42.294 1.384 0.31 26.27 C
1323 CG BLYS A of atom, 161-6.825 41.860 0.495 0.31 25.35 C
1324 CD BLYS A of atom, 161-7.570 43.059-0.021 0.31 26.49 C
1325 CE BLYS A of atom, 161-8.650 42.622-0.961 0.31 23.07 C
1326 NZ BLYS A of atom, 161-8.127 41.710-2.014 0.31 23.33 N
1327 N ASP A of atom, 162-4.759 41.838 4.819 1.00 25.68 N
1328 CA ASP A of atom, 162-4.870 42.403 6.157 1.00 28.56 C
1329 C ASP A of atom, 162-3.606 43.184 6.492 1.00 28.88 C
1330 O ASP A of atom, 162-3.525 44.387 6.218 1.00 30.53 O
1331 CB ASP A of atom, 162-5.160 41.313 7.199 1.00 29.07 C
1332 CG ASP A of atom, 162-4.432 40.013 6.911 1.00 27.75 C
1333 OD1 ASP A of atom, 162-3.217 40.053 6.630 1.00 29.09 O
1334 OD2 ASP A of atom, 162-5.076 38.942 6.964 1.00 30.35 O
1335 N HIS A of atom, 163-2.615 42.515 7.057 1.00 24.24 N
1336 CA HIS A of atom, 163-1.373 43.202 7.360 1.00 26.59 C
1337 C HIS A of atom, 163-0.698 43.639 6.077 1.00 27.71 C
1338 O HIS A of atom, 163-0.655 42.917 5.095 1.00 29.11 O
1339 CB HIS A of atom, 163-0.401 42.314 8.128 1.00 31.48 C
1340 CG HIS A of atom, 163-0.892 41.879 9.473 1.00 33.67 C
1341 ND1 HIS A of atom, 163-0.381 42.381 10.652 1.00 32.55 N
1342 CD2 HIS A of atom, 163-1.822 40.961 9.824 1.00 36.61 C
1343 CE1 HIS A of atom, 163-0.989 41.800 11.671 1.00 36.69 C
1344 NE2 HIS A of atom, 163-1.864 40.933 11.196 1.00 40.61 N
1345 N VAL A of atom, 164-0.154 44.835 6.104 1.00 26.44 N
1346 CA VAL A of atom, 164 0.571 45.399 4.968 1.00 27.88 C
1347 C VAL A of atom, 164 1.853 45.997 5.534 1.00 26.09 C
1348 O VAL A of atom, 164 1.848 47.126 6.038 1.00 32.33 O
1349 CB VAL A of atom, 164-0.254 46.448 4.205 1.00 27.23 C
1350 CG1 VAL A of atom, 164 0.610 47.196 3.192 1.00 24.79 C
1351 CG2 VAL A of atom, 164-1.428 45.789 3.504 1.00 29.29 C
1352 N HIS A of atom, 165 2.947 45.227 5.495 1.00 21.54 N
1353 CA HIS A of atom, 165 4.246 45.704 5.956 1.00 20.07 C
1354 C HIS A of atom, 165 5.106 46.033 4.756 1.00 18.53 C
1355 O HIS A of atom, 165 5.460 45.117 3.998 1.00 22.77 O
1356 CB HIS A of atom, 165 4.955 44.641 6.763 1.00 22.13 C
1357 CG HIS A of atom, 165 4.151 44.128 7.899 1.00 29.75 C
1358 ND1 HIS A of atom, 165 4.227 42.826 8.335 1.00 34.64 N
1359 CD2 HIS A of atom, 165 3.240 44.743 8.684 1.00 26.86 C
1360 CE1 HIS A of atom, 165 3.399 42.662 9.350 1.00 32.20 C
1361 NE2 HIS A of atom, 165 2.797 43.811 9.583 1.00 27.61 N
1362 N PRO A of atom, 166 5.510 47.281 4.567 1.00 13.92 N
1363 CA PRO A of atom, 166 6.410 47.594 3.451 1.00 13.35 C
1364 C PRO A of atom, 166 7.773 46.929 3.611 1.00 11.56 C
1365 O PRO A of atom, 166 8.297 46.796 4.719 1.00 14.08 O
1366 CB PRO A of atom, 166 6.533 49.123 3.514 1.00 12.32 C
1367 CG PRO A of atom, 166 5.312 49.567 4.308 1.00 20.34 C
1368 CD PRO A of atom, 166 5.088 48.480 5.313 1.00 16.34 C
1369 N THR A of atom, 167 8.356 46.518 2.484 1.00 11.67 N
1370 CA THR A of atom, 167 9.771 46.157 2.457 1.00 11.58 C
1371 C THR A of atom, 167 10.609 47.417 2.652 1.00 11.73 C
1372 O THR A of atom, 167 10.089 48.533 2.710 1.00 11.44 O
1373 CB THR A of atom, 167 10.133 45.536 1.113 1.00 11.18 C
1374 OG1 THR A of atom, 167 9.946 46.538 0.115 1.00 11.10 O
1375 CG2 THR A of atom, 167 9.241 44.329 0.795 1.00 12.93 C
1376 N ALA A of atom, 168 11.932 47.249 2.729 1.00 12.92 N
1377 CA ALA A of atom, 168 12.799 48.418 2.857 1.00 13.41 C
1378 C ALA A of atom, 168 12.616 49.364 1.675 1.00 12.24 C
1379 O ALA A of atom, 168 12.576 50.594 1.836 1.00 12.15 O
1380 CB ALA A of atom, 168 14.257 47.974 2.984 1.00 16.50 C
1381 N ARG A of atom, 169 12.470 48.803 0.476 1.00 11.42 N
1382 CA ARG A of atom, 169 12.209 49.620-0.698 1.00 11.91 C
1383 C ARG A of atom, 169 10.817 50.242-0.625 1.00 12.18 C
1384 O ARG A of atom, 169 10.622 51.396-1.020 1.00 11.20 O
1385 CB ARG A of atom, 169 12.388 48.758-1.949 1.00 13.09 C
1386 CG ARG A of atom, 169 12.209 49.461-3.264 1.00 15.94 C
1387 CD ARG A of atom, 169 13.300 50.491-3.547 1.00 24.40 C
1388 NE ARG A of atom, 169 12.984 51.219-4.774 1.00 27.09 N
1389 CZ ARG A of atom, 169 11.993 52.102-4.874 1.00 27.13 C
1390 NH1 ARG A of atom, 169 11.246 52.385-3.816 1.00 27.88 N
1391 NH2 ARG A of atom, 169 11.751 52.714-6.022 1.00 27.16 N
1392 N GLY A of atom, 170 9.841 49.493-0.104 1.00 10.43 N
1393 CA GLY A of atom, 170 8.522 50.065 0.112 1.00 10.60 C
1394 C GLY A of atom, 170 8.548 51.243 1.070 1.00 9.39 C
1395 O GLY A of atom, 170 7.831 52.224 0.872 1.00 10.77 O
1396 N VAL A of atom, 171 9.375 51.161 2.115 1.00 9.65 N
1397 CA VAL A of atom, 171 9.518 52.288 3.043 1.00 9.90 C
1398 C VAL A of atom, 171 10.021 53.519 2.307 1.00 10.48 C
1399 O VAL A of atom, 171 9.514 54.630 2.497 1.00 10.46 O
1400 CB VAL A of atom, 171 10.442 51.925 4.219 1.00 11.10 C
1401 CG1 VAL A of atom, 171 10.897 53.192 4.945 1.00 12.07 C
1402 CG2 VAL A of atom, 171 9.751 50.989 5.175 1.00 11.34 C
1403 N GLU A of atom, 172 11.023 53.342 1.436 1.00 11.47 N
1404 CA GLU A of atom, 172 11.533 54.478 0.675 1.00 12.28 C
1405 C GLU A of atom, 172 10.429 55.102-0.159 1.00 10.48 C
1406 O GLU A of atom, 172 10.365 56.326-0.312 1.00 12.23 O
1407 CB GLU A of atom, 172 12.718 54.038-0.188 1.00 14.42 C
1408 CG GLU A of atom, 172 13.899 53.598 0.654 1.00 16.84 C
1409 CD GLU A of atom, 172 15.062 53.087-0.169 1.00 26.14 C
1410 OE1 GLU A of atom, 172 14.913 52.946-1.403 1.00 26.18 O
1411 OE2 GLU A of atom, 172 16.126 52.820 0.428 1.00 29.13 O
1412 N AGLU A of atom, 173 9.543 54.273-0.714 0.53 10.74 N
1413 CA AGLU A of atom, 173 8.405 54.797-1.461 0.53 11.17 C
1414 C AGLU A of atom, 173 7.506 55.644-0.573 0.53 11.00 C
1415 O AGLU A of atom, 173 7.054 56.721-0.975 0.53 11.21 O
1416 CB AGLU A of atom, 173 7.597 53.641-2.034 0.53 13.54 C
1417 CG AGLU A of atom, 173 8.231 53.013-3.222 0.53 14.06 C
1418 CD AGLU A of atom, 173 8.262 53.943-4.390 0.53 17.96 C
1419 OE1 AGLU A of atom, 173 7.202 54.524-4.709 0.53 16.23 O
1420 OE2 AGLU A of atom, 173 9.346 54.099-4.982 0.53 15.14 O
1421 N BGLU A of atom, 173 9.546 54.264-0.705 0.47 10.76 N
1422 CA BGLU A of atom, 173 8.402 54.749-1.464 0.47 11.21 C
1423 C BGLU A of atom, 173 7.482 55.604-0.595 0.47 11.06 C
1424 O BGLU A of atom, 173 7.009 56.662-1.025 0.47 11.24 O
1425 CB BGLU A of atom, 173 7.655 53.537-2.022 0.47 13.45 C
1426 CG BGLU A of atom, 173 6.582 53.848-3.028 0.47 14.16 C
1427 CD BGLU A of atom, 173 7.142 54.386-4.314 0.47 17.86 C
1428 OE1 BGLU A of atom, 173 8.294 54.041-4.673 0.47 14.58 O
1429 OE2 BGLU A of atom, 173 6.424 55.163-4.972 0.47 14.24 O
1430 N LEU A of atom, 174 7.222 55.161 0.638 1.00 8.92 N
1431 CA LEU A of atom, 174 6.376 55.927 1.550 1.00 9.69 C
1432 C LEU A of atom, 174 7.042 57.233 1.939 1.00 9.85 C
1433 O LEU A of atom, 174 6.377 58.267 2.043 1.00 10.58 O
1434 CB LEU A of atom, 174 6.099 55.120 2.815 1.00 10.61 C
1435 CG LEU A of atom, 174 5.334 53.818 2.629 1.00 10.63 C
1436 CD1 LEU A of atom, 174 5.259 53.073 3.957 1.00 17.31 C
1437 CD2 LEU A of atom, 174 3.944 54.091 2.094 1.00 11.93 C
1438 N VAL A of atom, 175 8.354 57.190 2.173 1.00 9.05 N
1439 CA VAL A of atom, 175 9.088 58.402 2.527 1.00 10.08 C
1440 C VAL A of atom, 175 9.009 59.418 1.394 1.00 9.91 C
1441 O VAL A of atom, 175 8.767 60.610 1.617 1.00 11.49 O
1442 CB VAL A of atom, 175 10.540 58.050 2.906 1.00 9.82 C
1443 CG1 VAL A of atom, 175 11.383 59.302 3.024 1.00 12.59 C
1444 CG2 VAL A of atom, 175 10.568 57.281 4.209 1.00 11.17 C
1445 N ASER A of atom, 176 9.187 58.963 0.152 0.69 10.53 N
1446 CA ASER A of atom, 176 9.134 59.890-0.974 0.69 11.61 C
1447 C ASER A of atom, 176 7.760 60.525-1.109 0.69 10.72 C
1448 O ASER A of atom, 176 7.651 61.691-1.514 0.69 12.63 O
1449 CB ASER A of atom, 176 9.532 59.179-2.268 0.69 13.09 C
1450 OG ASER A of atom, 176 10.893 58.799-2.215 0.69 13.20 O
1451 N BSER A of atom, 176 9.184 58.956 0.152 0.31 10.67 N
1452 CA BSER A of atom, 176 9.134 59.866-0.989 0.31 11.69 C
1453 C BSER A of atom, 176 7.761 60.503-1.143 0.31 10.80 C
1454 O BSER A of atom, 176 7.655 61.646-1.604 0.31 12.75 O
1455 CB BSER A of atom, 176 9.537 59.135-2.274 0.31 13.15 C
1456 OG BSER A of atom, 176 8.542 58.211-2.685 0.31 13.49 O
1457 N ALA A of atom, 177 6.700 59.794-0.753 1.00 10.41 N
1458 CA ALA A of atom, 177 5.350 60.313-0.903 1.00 10.06 C
1459 C ALA A of atom, 177 4.913 61.217 0.241 1.00 11.06 C
1460 O ALA A of atom, 177 3.998 62.024 0.054 1.00 13.49 O
1461 CB ALA A of atom, 177 4.357 59.156-1.008 1.00 12.58 C
1462 N THR A of atom, 178 5.544 61.119 1.414 1.00 9.55 N
1463 CA THR A of atom, 178 5.057 61.807 2.606 1.00 10.26 C
1464 C THR A of atom, 178 6.022 62.833 3.186 1.00 10.17 C
1465 O THR A of atom, 178 5.632 63.557 4.115 1.00 10.56 O
1466 CB THR A of atom, 178 4.690 60.796 3.707 1.00 9.62 C
1467 OG1 THR A of atom, 178 5.846 60.036 4.068 1.00 10.59 O
1468 CG2 THR A of atom, 178 3.579 59.855 3.221 1.00 13.64 C
1469 N SER A of atom, 179 7.258 62.927 2.681 1.00 10.72 N
1470 CA SER A of atom, 179 8.217 63.838 3.299 1.00 11.00 C
1471 C SER A of atom, 179 7.792 65.291 3.150 1.00 10.57 C
1472 O SER A of atom, 179 8.049 66.101 4.046 1.00 10.62 O
1473 CB SER A of atom, 179 9.607 63.638 2.709 1.00 10.83 C
1474 OG SER A of atom, 179 10.203 62.454 3.197 1.00 11.85 O
1475 N ASN A of atom, 180 7.162 65.650 2.028 1.00 9.59 N
1476 CA ASN A of atom, 180 6.661 67.013 1.868 1.00 11.71 C
1477 C ASN A of atom, 180 5.698 67.359 2.989 1.00 11.58 C
1478 O ASN A of atom, 180 5.773 68.444 3.574 1.00 12.02 O
1479 CB ASN A of atom, 180 5.937 67.166 0.528 1.00 12.94 C
1480 CG ASN A of atom, 180 6.885 67.202-0.667 1.00 18.16 C
1481 OD1 ASN A of atom, 180 8.084 67.405-0.518 1.00 19.11 O
1482 ND2 ASN A of atom, 180 6.337 67.007-1.867 1.00 17.71 N
1483 N ALA A of atom, 181 4.776 66.445 3.299 1.00 10.56 N
1484 CA ALA A of atom, 181 3.793 66.712 4.346 1.00 10.80 C
1485 C ALA A of atom, 181 4.462 66.865 5.704 1.00 9.46 C
1486 O ALA A of atom, 181 4.063 67.717 6.510 1.00 11.90 O
1487 CB ALA A of atom, 181 2.762 65.584 4.387 1.00 13.72 C
1488 N VAL A of atom, 182 5.470 66.039 5.984 1.00 9.76 N
1489 CA VAL A of atom, 182 6.163 66.122 7.263 1.00 9.85 C
1490 C VAL A of atom, 182 6.909 67.444 7.379 1.00 9.49 C
1491 O VAL A of atom, 182 6.846 68.131 8.410 1.00 10.97 O
1492 CB VAL A of atom, 182 7.117 64.929 7.433 1.00 8.87 C
1493 CG1 VAL A of atom, 182 7.940 65.095 8.706 1.00 10.74 C
1494 CG2 VAL A of atom, 182 6.352 63.615 7.453 1.00 11.20 C
1495 N ALA A of atom, 183 7.632 67.817 6.315 1.00 9.90 N
1496 CA ALA A of atom, 183 8.389 69.066 6.333 1.00 10.92 C
1497 C ALA A of atom, 183 7.467 70.260 6.505 1.00 12.77 C
1498 O ALA A of atom, 183 7.806 71.219 7.213 1.00 14.12 O
1499 CB ALA A of atom, 183 9.208 69.207 5.049 1.00 12.40 C
1500 N ALYS A of atom, 184 6.281 70.205 5.890 0.50 11.99 N
1501 CA ALYS A of atom, 184 5.314 71.297 5.981 0.50 15.11 C
1502 C ALYS A of atom, 184 4.667 71.372 7.358 0.50 15.29 C
1503 O ALYS A of atom, 184 4.299 72.462 7.813 0.50 16.83 O
1504 CB ALYS A of atom, 184 4.251 71.131 4.890 0.50 15.81 C
1505 CG ALYS A of atom, 184 3.003 72.000 5.043 0.50 19.31 C
1506 CD ALYS A of atom, 184 2.104 71.894 3.815 0.50 22.85 C
1507 CE ALYS A of atom, 184 0.896 72.815 3.928 0.50 25.54 C
1508 NZ ALYS A of atom, 184 0.166 72.940 2.636 0.50 25.39 N
1509 N BLYS A of atom, 184 6.296 70.231 5.871 0.50 11.98 N
1510 CA BLYS A of atom, 184 5.363 71.341 6.027 0.50 15.13 C
1511 C BLYS A of atom, 184 4.799 71.395 7.441 0.50 15.20 C
1512 O BLYS A of atom, 184 4.629 72.483 8.007 0.50 15.35 O
1513 CB BLYS A of atom, 184 4.238 71.241 4.998 0.50 15.74 C
1514 CG BLYS A of atom, 184 4.664 71.534 3.569 0.50 17.74 C
1515 CD BLYS A of atom, 184 3.472 71.510 2.626 0.50 20.49 C
1516 CE BLYS A of atom, 184 3.869 71.009 1.248 0.50 23.98 C
1517 NZ BLYS A of atom, 184 2.806 70.159 0.653 0.50 26.21 N
1518 N ALA A of atom, 185 4.514 70.232 8.031 1.00 11.70 N
1519 CA ALA A of atom, 185 3.921 70.203 9.362 1.00 11.70 C
1520 C ALA A of atom, 185 4.893 70.641 10.452 1.00 11.67 C
1521 O ALA A of atom, 185 4.447 71.071 11.525 1.00 12.84 O
1522 CB ALA A of atom, 185 3.386 68.804 9.671 1.00 12.03 C
1523 N LEU A of atom, 186 6.194 70.503 10.231 1.00 11.76 N
1524 CA LEU A of atom, 186 7.180 70.901 11.227 1.00 11.46 C
1525 C LEU A of atom, 186 7.317 72.416 11.204 1.00 12.36 C
1526 O LEU A of atom, 186 7.673 72.975 10.160 1.00 13.46 O
1527 CB LEU A of atom, 186 8.523 70.271 10.882 1.00 12.42 C
1528 CG LEU A of atom, 186 9.690 70.599 11.821 1.00 12.97 C
1529 CD1 LEU A of atom, 186 9.475 69.998 13.219 1.00 13.01 C
1530 CD2 LEU A of atom, 186 11.003 70.118 11.216 1.00 15.31 C
1531 N PRO A of atom, 187 7.052 73.118 12.310 1.00 12.88 N
1532 CA PRO A of atom, 187 7.197 74.581 12.290 1.00 14.93 C
1533 C PRO A of atom, 187 8.616 74.993 11.927 1.00 15.61 C
1534 O PRO A of atom, 187 9.589 74.334 12.293 1.00 16.27 O
1535 CB PRO A of atom, 187 6.850 74.989 13.725 1.00 17.96 C
1536 CG PRO A of atom, 187 5.965 73.904 14.225 1.00 17.16 C
1537 CD PRO A of atom, 187 6.492 72.638 13.590 1.00 12.98 C
1538 N ALA A of atom, 188 8.728 76.099 11.200 1.00 20.65 N
1539 CA ALA A of atom, 188 10.032 76.589 10.765 1.00 23.44 C
1540 C ALA A of atom, 188 10.825 77.167 11.933 1.00 24.88 C
1541 O ALA A of atom, 188 10.315 77.286 13.048 1.00 27.41 O
1542 CB ALA A of atom, 188 9.864 77.632 9.667 1.00 29.70 C
TER 1543 ALA A 188
Sequence table
<110>Fudan University
<120>the thermophilic esterase crystal three-dimensional structure and expression and purification method of marine bacteria are derived from
<130> 001
<160> 4
<170> SIPOSequenceListing 1.0
<210> 1
<211> 190
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 1
Met Gly Glu Ser Arg Val Ile Leu Ala Phe Gly Asp Ser Leu Phe Ala
1 5 10 15
Gly Tyr Gly Leu Asp Lys Gly Glu Ser Tyr Pro Ala Lys Leu Glu Thr
20 25 30
Ala Leu Arg Ser His Gly Ile Asn Ala Arg Ile Ile Asn Ala Gly Val
35 40 45
Ser Gly Asp Thr Thr Ala Ala Gly Leu Gln Arg Ile Lys Phe Val Leu
50 55 60
Asp Ser Gln Pro Asp Lys Pro Glu Leu Ala Ile Val Glu Leu Gly Gly
65 70 75 80
Asn Asp Leu Leu Arg Gly Leu Ser Pro Ala Glu Ala Arg Gln Asn Leu
85 90 95
Ser Gly Ile Leu Glu Glu Leu Gln Arg Arg Lys Ile Pro Ile Leu Leu
100 105 110
Met Gly Met Arg Ala Pro Pro Asn Leu Gly Ala Lys Tyr Gln Arg Glu
115 120 125
Phe Asp Gly Ile Tyr Pro Tyr Leu Ala Glu Lys Tyr Asp Ala Lys Leu
130 135 140
Val Pro Phe Phe Leu Glu Ala Val Ala Asp Arg Pro Asp Leu Ile Gln
145 150 155 160
Lys Asp His Val His Pro Thr Ala Arg Gly Val Glu Glu Leu Val Ser
165 170 175
Ala Thr Ser Asn Ala Val Ala Lys Ala Leu Pro Ala Lys Lys
180 185 190
<210> 2
<211> 573
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 2
atgggcgaat cgcgcgtgat tctcgccttc ggagacagcc tgtttgcagg ctatggcctt 60
gataaggggg agagctatcc ggcaaagctg gaaactgcgc tgcgcagcca tggcatcaat 120
gccagaatca ttaatgccgg cgtttcgggt gacaccactg cggcagggct gcagcgaatc 180
aaattcgtgc tggatagcca gccggacaag ccggaattgg ccatagtgga actgggcggg 240
aatgaccttt tacgcggcct ctcaccagcc gaagcgcggc agaacctcag cggaatcctc 300
gaagaattgc agaggcggaa aattccaatc ctgttgatgg gaatgcgagc gccgcccaat 360
ctaggggcaa aatatcagcg cgaatttgat gggatttatc cctatctggc cgaaaaatat 420
gacgccaagc tggtaccttt cttccttgag gccgtggcag atagacctga cctcattcag 480
aaggatcacg ttcaccccac tgcgcgcggt gtggaggaac tcgtgtctgc aacatcgaat 540
gcagttgcca aggcgctgcc tgcgaagaag tga 573
<210> 3
<211> 24
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 3
tcgcggatcc atgggcgaat cgcg 24
<210> 4
<211> 24
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 4
tccgctcgag tcacttcttc gcag 24

Claims (7)

1. a kind of thermophilic esterase crystal three-dimensional structure from marine bacteria, the marine bacteria areAltererythrobacterindicus DSM 18604T, thermophilic esterase AlinE4 contains 190 amino acid, molecule Amount is 20.5 kDa；It is characterized in that, the atom in crystal three-dimensional structure has at least 40% atomic coordinates listed in table 1, Or the average root variance of the atomic structure coordinate of the main chain carbon skeleton of at least 40% amino acid and the coordinate in table 1 is less than or waits In 1.2.
2. thermophilic esterase crystal three-dimensional structure according to claim 1, which is characterized in that resolution ratio 1.18；Wherein, The crystal structure space group of the esterase AlinE4 is P 42 212, an asymmetry unit contains a molecule；Cell parameter Are as follows: a=79.783, b=79.783, c=61.010, α=β=γ=90.000 °.
3. thermophilic esterase crystal three-dimensional structure according to claim 1, which is characterized in that by 5 β-pleated sheets, 9 α spiral shells and 2 A 310Spiral rotation composition；
5 β-pleated sheets are located at center, and 9 α are spiraled about around β-pleated sheet；Wherein, β-pleated sheet 1 contains The amino acid section of Arg5-Gly11；α spiral 1, i.e. the amino acid section containing Ser13-Ala16；α spiral 2 contains Tyr26- The amino acid section of His37；β-pleated sheet 2, i.e. the amino acid section containing Ala41-Gly47；α spiral 3, i.e., containing Thr53-Asp65's Amino acid section；β-pleated sheet 3, i.e. the amino acid section containing Leu73-Glu77；α spiral 4, i.e., containing the amino acid of Gly80-Leu84 Section；α spiral 5, i.e. the amino acid section containing Pro89-Arg105；β-pleated sheet 4, i.e. the amino acid area containing Ile110-Met113 Section；310Spiral 1, i.e. the amino acid section containing Pro119-Leu121；α spiral 6, i.e. the amino acid area containing Ala123-Asp130 Section；α spiral 7, i.e. the amino acid section containing Ile132-Lys139；β-pleated sheet 5, i.e. the amino acid section containing Lys143-Val145； α spiral 8, i.e. the amino acid section containing Phe148-Val152；310Spiral 3, i.e. the amino acid section containing Pro156-Leu158；α Spiral 9, i.e. the amino acid section containing Ala168-Ala185.
4. thermophilic esterase crystal three-dimensional structure according to claim 1, which is characterized in that by the 13rd serine, the 162nd Position aspartic acid and the 165th hyte propylhomoserin constitute the activated centre of three disjunctors of catalysis；Activated centre is occupied by a cadmium ion.
5. thermophilic esterase crystal three-dimensional structure according to claim 1, which is characterized in that by the 13rd serine, the 50th Position glycine and the 81st asparagine constitute oxonium ion channel.
6. one kind derives from marine bacteriaAltererythrobacter indicus DSM 18604TEsterase AlinE4 table It reaches and purification process, which is characterized in that specific steps are as follows: wild type has been merged in buildingaline4The recombination of full-length gene carries Body, the carrier are converted to Escherichia coli, so that expression has the fusion protein AlinE4 of label, the albumen of above-mentioned acquisition is passed through The specific method with the special tag recognition isolates destination protein, then obtains height by the method for gel permeation chromatography The albumen of the esterase AlinE4 of purity；
The upstream primer for expanding esterase full genome is SEQ.ID.NO3 and downstream primer is SEQ.ID.NO4；Expression plasmid is PSMT3, method for transformation are CaCl2 conversion method, and conversion bacterial strain is bacillus coli DH 5 alpha, and expression bacterial strain is e. coli bl21 (DE3)。
7. one kind derives from marine bacteriaAltererythrobacter indicus DSM 18604TEsterase AlinE4 knot Crystal method, which is characterized in that the specific steps are it is 10-60 mg/ml that albumin A linE4, which is concentrated into concentration, uses crystalline reagents Primary dcreening operation condition of the box as crystal growth, is crystallized using hanging drop vapor phase grafting, and temperature is 4-25 degrees Celsius.
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110184254A (en) * 2019-03-21 2019-08-30 复旦大学 It is a kind of with the esterase mutant of high alkali resistance and its application
CN110184254B (en) * 2019-03-21 2022-07-22 复旦大学 Esterase mutant with high alkali resistance and application thereof

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