CN109828075A - The discrimination method of pterygospermin and spirochin in a kind of leaf of Moringa - Google Patents
The discrimination method of pterygospermin and spirochin in a kind of leaf of Moringa Download PDFInfo
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- CN109828075A CN109828075A CN201910230489.4A CN201910230489A CN109828075A CN 109828075 A CN109828075 A CN 109828075A CN 201910230489 A CN201910230489 A CN 201910230489A CN 109828075 A CN109828075 A CN 109828075A
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Abstract
The present invention relates to the discrimination methods of pterygospermin and spirochin in a kind of leaf of Moringa, it is characterized in that by after leaf of Moringa water extract LSA5B type purification with macroreticular resin, identified in 0~10 DEG C with thin-layered chromatography, the exclusive discrimination method of pterygospermin and spirochin in a kind of leaf of Moringa is provided, Moringa leaf preparation is made effectively to ensure drug quality in production and application and curative effect.
Description
Technical field
The present invention relates to compound discrimination methods, and in particular to pterygospermin and spirochin in a kind of leaf of Moringa
Discrimination method belongs to field of pharmaceutical technology.
Background technique
Moringa (Moringa oleiferamLam. India and African Territories) are originated in, are Moringaceae Moringa platymiscium.Mesh
It is preceding to be planted extensively in Asia, African subtropical and tropical zones, because growth is rapid, full of nutrition, medical value is high for it, it is known as
" tree of miracle ", " medical treasure case " etc..
Leaf of Moringa nutriment rich in and medicinal ingredient, studies have shown that leaf of Moringa powder has antibacterial, anti-inflammatory etc. medicinal
Effect, effective component is mainly alkaloid, and alkaloid is distributed in the content in leaf and is significantly higher than stem, and total alkaloid is up to
4.62mg/g.The study found that the alkaloid in leaf of Moringa to Escherichia coli, Pseudomonas aeruginosa, staphylococcus aureus and produces gas intestines
There is coccus etc. broad-spectrum antibacterial effect, especially pterygospermin and spirochin to have apparent bactericidal effect, can be used to
Produce bacteriostatic agent.
Existing literature is more to the discrimination method of alkaloid, but seldom to the identification of alkaloid in leaf of Moringa, more has no pair
The report that its major antibacterial ingredient pterygospermin and spirochin identify.It cannot effectively guarantee to produce in production or use
Quality, therefore pharmaceutical effectiveness cannot be guaranteed.
Since both pterygospermin and spirochin chemical property are very much like, it is difficult separation and identifies, we
By a series of researchs, discovery is using after first being purified leaf of Moringa water, then TLC twice is unfolded in 0~10 DEG C
Method can identify pterygospermin in leaf of Moringa and spirochin.
Summary of the invention
The object of the present invention is to provide the discrimination methods of pterygospermin and spirochin in a kind of leaf of Moringa.Institute
The discrimination method of pterygospermin and spirochin is as follows in the leaf of Moringa stated: by leaf of Moringa water extract LSA5B type macropore
Purification on adsorbent resins will be used as test liquid after the concentration of chloroform eluent.Pterygospermin and spirochin reference substance are taken respectively
It is dissolved in chloroform, as contrast solution.By test liquid and contrast solution with capillary point in same silica HF 254 thin-layer chromatography glass
On plate, at room temperature after a certain period of time with the presaturation of ammonia steam, then with chlorofonn-ethylacetate-glacial acetic acid-water in 0~10 DEG C
(3.5:2:0.03:0.5) is that solvent is unfolded twice, and spray is after 105 DEG C of dryings to improve the exclusive colour developing of bismuth potassium iodide alkaloid
Agent is inspected in the sunlight.
Preferably, the test liquid is first water-soluble with a large amount of pigments of LSA5B type macroporous absorbent resin elution removing, polysaccharide etc.
Property impurity.
Preferably, 15~25min of the ammonia steam presaturation.
Preferably, described that temperature is unfolded for solvent with chlorofonn-ethylacetate-glacial acetic acid-water (3.5:2:0.03:0.5)
It is 0~10 DEG C.
Preferably, described that number is unfolded for solvent with chlorofonn-ethylacetate-glacial acetic acid-water (3.5:2:0.03:0.5)
For twice.
Preferably, it is described with chlorofonn-ethylacetate-glacial acetic acid-water (3.5:2:0.03:0.5) be solvent, for the first time
It is expanded to the 1/3~2/3 of lamellae forward position, is expanded to the 3/4~1 of lamellae frontal line for the second time.
Detailed description of the invention
Fig. 1: 1 thin layer of embodiment identifies figure: 1,2,4 for the three batches of leaf of Moringa test liquids (lot number be respectively 7L1103,
7N0122,8L1005), 3 be reference substance solution.
Fig. 2: 2 thin layer of embodiment identifies figure: 1,2,4 for the three batches of leaf of Moringa test liquids (lot number be respectively 7L1103,
7N0122,8L1005), 3 be reference substance solution.
Fig. 3: 3 thin layer of embodiment identifies figure: 1,3,4 for the three batches of leaf of Moringa test liquids (lot number be respectively 7L1103,
7N0122,8L1005), 2 be reference substance solution.
Specific embodiment
Leaf of Moringa water extract LSA5B type purification with macroreticular resin is first removed a large amount of pigments, sugar with water by embodiment 1
Class and water soluble protein, then eluted with chloroform, test liquid will be used as after the concentration of chloroform eluent.Pterygospermin pair is taken respectively
It is dissolved in chloroform according to product and spirochin reference substance, as reference substance solution.By test liquid and reference substance solution capillary point
On same silica HF 254 thin-layer chromatography glass plate, at room temperature with after ammonia steam presaturation 20min, then in 0~10 DEG C with
Chlorofonn-ethylacetate-glacial acetic acid-water (3.5:2:0.03:0.5) is that solvent is unfolded twice, before being expanded to lamellae for the first time
The 1/2 of edge is expanded to lamellae frontal line for the second time, and it is exclusive to improve bismuth potassium iodide alkaloid to take out the spray after 105 DEG C of dryings
Color developing agent is inspected in the sunlight.
Leaf of Moringa water extract LSA5B type purification with macroreticular resin is first removed a large amount of pigments, sugar with water by embodiment 2
Class and water soluble protein, then eluted with chloroform, test liquid will be used as after the concentration of chloroform eluent.Pterygospermin pair is taken respectively
It is dissolved in chloroform according to product and spirochin reference substance, as reference substance solution.By test liquid and reference substance solution capillary point
On same silica HF 254 thin-layer chromatography glass plate, at room temperature with after ammonia steam presaturation 25min, then in 0~10 DEG C with
Chlorofonn-ethylacetate-glacial acetic acid-water (3.5:2:0.03:0.5) is that solvent is unfolded twice, before being expanded to lamellae for the first time
The 1/3 of edge is expanded to the 4/5 of lamellae frontal line for the second time, takes out and sprays after 105 DEG C of dryings to improve bismuth potassium iodide alkaloid
Exclusive color developing agent, is inspected in the sunlight.
Leaf of Moringa water extract LSA5B type purification with macroreticular resin is first removed a large amount of pigments, sugar with water by embodiment 3
Class and water soluble protein, then eluted with chloroform, test liquid will be used as after the concentration of chloroform eluent.Pterygospermin pair is taken respectively
It is dissolved in chloroform according to product and spirochin reference substance, as reference substance solution.By test liquid and reference substance solution capillary point
On same silica HF 254 thin-layer chromatography glass plate, at room temperature with after ammonia steam presaturation 15min, then in 0~10 DEG C with
Chlorofonn-ethylacetate-glacial acetic acid-water (3.5:2:0.03:0.5) is that solvent is unfolded twice, before being expanded to lamellae for the first time
The 2/3 of edge is expanded to lamellae frontal line for the second time, and it is exclusive to improve bismuth potassium iodide alkaloid to take out the spray after 105 DEG C of dryings
Color developing agent is inspected in the sunlight.
Claims (6)
1. the discrimination method of pterygospermin and spirochin in a kind of leaf of Moringa, its step are as follows: by leaf of Moringa water extract
With LSA5B type purification with macroreticular resin, it will be used as test liquid after the concentration of chloroform eluent, take pterygospermin and phoenix tail respectively
Pterigospermin reference substance is dissolved in chloroform, as contrast solution, by test liquid and contrast solution with capillary point in same silica HF 254
On thin-layer chromatography glass plate, at room temperature after a certain period of time with the presaturation of ammonia steam, then with chloroform-acetic acid second in 0~10 DEG C
Ester-glacial acetic acid-water (3.5:2:0.03:0.5) is that solvent is unfolded twice, is sprayed after 105 DEG C of dryings to improve bismuth potassium iodide biology
The exclusive color developing agent of alkali, is inspected in the sunlight.
2. the discrimination method of pterygospermin and spirochin in leaf of Moringa as described in claim 1, which is characterized in that supply
Test solution is first to remove the water-solubility impurities such as a large amount of pigments, polysaccharide with the elution of LSA5B type macroporous absorbent resin.
3. the discrimination method of pterygospermin and spirochin in leaf of Moringa as described in claim 1, which is characterized in that ammonia
15~25min of steam presaturation.
4. the discrimination method of pterygospermin and spirochin in leaf of Moringa as described in claim 1, which is characterized in that exhibition
Opening temperature is 0~10 DEG C.
5. the discrimination method of pterygospermin and spirochin in leaf of Moringa as described in claim 1, which is characterized in that with
Chlorofonn-ethylacetate-glacial acetic acid-water (3.5:2:0.03:0.5) is that solvent expansion number is twice.
6. the discrimination method of pterygospermin and spirochin in leaf of Moringa as described in claim 1, which is characterized in that with
Chlorofonn-ethylacetate-glacial acetic acid-water (3.5:2:0.03:0.5) is solvent, is expanded to the 1/3 of lamellae forward position for the first time
~2/3, it is expanded to the 3/4~1 of lamellae frontal line for the second time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910230489.4A CN109828075B (en) | 2019-03-26 | 2019-03-26 | Method for identifying indian moringa oleifera and phoenix tail moringa oleifera in moringa leaves |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910230489.4A CN109828075B (en) | 2019-03-26 | 2019-03-26 | Method for identifying indian moringa oleifera and phoenix tail moringa oleifera in moringa leaves |
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| Publication Number | Publication Date |
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| CN109828075A true CN109828075A (en) | 2019-05-31 |
| CN109828075B CN109828075B (en) | 2020-12-01 |
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| CN201910230489.4A Active CN109828075B (en) | 2019-03-26 | 2019-03-26 | Method for identifying indian moringa oleifera and phoenix tail moringa oleifera in moringa leaves |
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Citations (5)
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| CA2821700A1 (en) * | 2012-08-01 | 2014-02-01 | General Electric Company | Material and exhaust gas system and method for using the same |
| CN103980291A (en) * | 2014-04-29 | 2014-08-13 | 天津大学 | Method for extracting pterygsspermine from moringa oleifera lam roots |
| CN104490954A (en) * | 2014-12-24 | 2015-04-08 | 广州泽力医药科技有限公司 | Method for extracting moringa oleifera |
| CN107184621A (en) * | 2017-05-16 | 2017-09-22 | 云南省林业科学院 | A kind of extracting method of leaf of Moringa active ingredient and its application |
| CN107586303A (en) * | 2017-11-03 | 2018-01-16 | 隆安县荣胜养殖有限公司 | Method for extracting and purifying moringa oleifera from moringa oleifera roots |
-
2019
- 2019-03-26 CN CN201910230489.4A patent/CN109828075B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2821700A1 (en) * | 2012-08-01 | 2014-02-01 | General Electric Company | Material and exhaust gas system and method for using the same |
| CN103980291A (en) * | 2014-04-29 | 2014-08-13 | 天津大学 | Method for extracting pterygsspermine from moringa oleifera lam roots |
| CN104490954A (en) * | 2014-12-24 | 2015-04-08 | 广州泽力医药科技有限公司 | Method for extracting moringa oleifera |
| CN107184621A (en) * | 2017-05-16 | 2017-09-22 | 云南省林业科学院 | A kind of extracting method of leaf of Moringa active ingredient and its application |
| CN107586303A (en) * | 2017-11-03 | 2018-01-16 | 隆安县荣胜养殖有限公司 | Method for extracting and purifying moringa oleifera from moringa oleifera roots |
Non-Patent Citations (4)
| Title |
|---|
| SHIKHA MEHTA 等: "Role of Spectral Studies in Detection of Antibacterial Phytoelements and Phytochemicals of Moringa oleifera", 《FOOD BIOPHYSICS》 * |
| 刘红 等: "辣木籽仁的化学成分分析", 《热带农业工程》 * |
| 郭利群 等: "辣木的药用价值及应用研究", 《热带农业科学》 * |
| 陈瑞娇 等: "辣木叶总黄酮的提取及其降血糖作用", 《食品与生物技术学报》 * |
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