CN109825298A - The method of paper chip the detection silver ion and serine of CdTe quantum based on dimercaptosuccinic acid modification - Google Patents
The method of paper chip the detection silver ion and serine of CdTe quantum based on dimercaptosuccinic acid modification Download PDFInfo
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Abstract
The present invention relates to a kind of methods of paper chip sensory field more particularly to paper chip the detection silver ion and serine of CdTe quantum based on dimercaptosuccinic acid modification.The method of CdTe quantum paper chip detection silver ion based on dimercaptosuccinic acid modification, which is characterized in that comprising steps of the preparation of the CdTe quantum of (1) dimercaptosuccinic acid modification；(2) preparation of the paper chip of the CdTe quantum of dimercaptosuccinic acid modification；(3) silver ion standard color comparison card is made；(4) silver ion of sample is detected.Compared with the detection method of existing silver ion and serine, the present invention is based on paper chip detection silver ion and serine have the characteristics that preparation it is simple, can rapid field detection, at low cost, fast response time, sensitivity and selectively high, and paper chip has specific reaction to silver ion and serine, can be used for the detection of silver ion or serine in complex matrices water sample.
Description
Technical field
The present invention relates to paper chip sensory field more particularly to a kind of CdTe quantums based on dimercaptosuccinic acid modification
The method of paper chip detection silver ion and serine.
Background technique
Water-soluble quantum dot has good photostability, fluorescence lifetime is long, well as outstanding fluorescent nano material
Biocompatibility, be a kind of ideal fluorescence probe the advantages that biggish Stokes shift.It, will based on its fluorescent characteristic
It is used for environmental monitoring, medical diagnosis, clinical medicine etc. as efficient fluorescence probe.It is industrialized with China
Develop, the silver ion pollution problem in drinking water, food becomes increasingly conspicuous, and certain threat is caused to human health.In addition, the U.S.
Bureau for Environmental Protection (EPA) points out that the toxic level of silver in drinking water ion is 460nM, U.S. government's industrial hygiene meeting
(ACGIH) the biology exposure index of the silver ion defined in blood is 2 μM, therefore, drinking water, food, the biological sample of monitoring
The concentration of silver ion is vital in product.Traditional detection method includes Atomic absorption/emission spectrum, plasma mass
With polarography etc., they are directed to the instrument of complex precise, cumbersome operating process etc., make its application by certain limit
System, is insufficient for the needs that silver ion quickly detects on the spot, therefore, it is necessary to establish it is a kind of simple, economical, quickly, can be with
The Silver detection method detected on the spot.
Summary of the invention
In order to solve problem above, the object of the present invention is to provide a kind of CdTe quantums based on dimercaptosuccinic acid modification
Paper chip detection silver ion and serine method, silver ion and serine can not only be detected fast, in high sensitivity, and
It can be realized and exempt from the detection immediately of instrument scene.
To achieve the above object, the paper chip of the CdTe quantum of the dimercaptosuccinic acid modification designed by the present invention, including
Paper base material and the CdTe quantum of the dimercaptosuccinic acid being fixed on paper base material modification, the CdTe of the dimercaptosuccinic acid modification
Quantum dot emission feux rouges and launch wavelength are 620~640nm.
Preferably, the concentration of the CdTe quantum of the dimercaptosuccinic acid modification is 20~25 μm of ol/L, sulfydryl
The partial size of the CdTe quantum of succinic acid modification is 3~5nm.
The preparation method of the paper chip of the CdTe quantum of dimercaptosuccinic acid modification, comprising steps of
(1) preparation of the CdTe quantum of dimercaptosuccinic acid modification
Dichloride cadmium and dimercaptosuccinic acid are dissolved in pure water, sequentially add tellurious after pH value of solution is adjusted to 10.5~11.5
Sour sodium and sodium borohydride, stir evenly, and finally react 40~60 minutes solution in 180~220 DEG C of baking oven, are emitted
The CdTe quantum for the dimercaptosuccinic acid modification to glow that wavelength is 620~640nm；
(2) preparation of the paper chip of the CdTe quantum of dimercaptosuccinic acid modification
The CdTe quantum of dimercaptosuccinic acid modification is added dropwise in paper base material, paper base material absorbs fixed dimercaptosuccinic acid modification
CdTe quantum after up to dimercaptosuccinic acid modification CdTe quantum paper chip.
Preferably, the concentration of the CdTe quantum of the dimercaptosuccinic acid modification is 20~25 μm of ol/L, sulfydryl
The partial size of the CdTe quantum of succinic acid modification is 3~5nm.
Preferably, in the step (2), the CdTe quantum dripping quantity of dimercaptosuccinic acid modification is 8~10uL,
Paper base material is the circular filter paper that diameter is 4~6mm, and the paper chip that the CdTe quantum of dimercaptosuccinic acid modification has been added dropwise is placed in
In 36~39 DEG C of baking oven, dry 5~7 minutes.
The method of CdTe quantum paper chip detection silver ion based on dimercaptosuccinic acid modification, which is characterized in that including
Step:
(1) preparation of the CdTe quantum of dimercaptosuccinic acid modification
Dichloride cadmium and dimercaptosuccinic acid are dissolved in pure water, sequentially add tellurious after pH value of solution is adjusted to 10.5~11.5
Sour sodium and sodium borohydride, stir evenly, and finally react 40~60 minutes solution in 180~220 DEG C of baking oven, are sent out
The CdTe quantum for the dimercaptosuccinic acid modification of a length of 620~640nm of ejected wave to glow；
(2) preparation of the paper chip of the CdTe quantum of dimercaptosuccinic acid modification
The CdTe quantum of dimercaptosuccinic acid modification is added dropwise in paper base material, paper base material absorbs fixed dimercaptosuccinic acid modification
CdTe quantum after up to dimercaptosuccinic acid modification CdTe quantum paper chip；
(3) silver ion standard color comparison card is made
The silver ion solution of various concentration is successively added dropwise in the paper chip of the CdTe quantum of dimercaptosuccinic acid modification,
The various concentration silver ion color different from paper chip reaction generation, takes pictures to each paper chip, and arrangement obtains silver ion
Standard color comparison card；
(4) silver ion of sample is detected
Sample is added dropwise in the paper chip for the CdTe quantum that dimercaptosuccinic acid is modified, paper chip has color to ring on sample
It answers, control silver ion standard color comparison card obtains the concentration of silver ion in sample.
Preferably, in the step (3), various concentration silver ion is reacted with paper chip and generates different colours
Photo imports the color data in PaintShop extraction picture, simulates silver ion standard color comparison card using color data.
Preferably, the concentration of silver ion is 0~1000nM in the step (3).
The method of the paper chip detection serine of CdTe quantum based on dimercaptosuccinic acid modification, which is characterized in that packet
Include step:
(1) preparation of the CdTe quantum of dimercaptosuccinic acid modification
Dichloride cadmium and dimercaptosuccinic acid are dissolved in pure water, sequentially add tellurious after pH value of solution is adjusted to 10.5~11.5
Sour sodium and sodium borohydride, stir evenly, and finally react 40~60 minutes solution in 180~220 DEG C of baking oven, are sent out
The CdTe quantum for the dimercaptosuccinic acid modification of a length of 620~640nm of ejected wave to glow；
(2) preparation of the paper chip of the CdTe quantum of dimercaptosuccinic acid modification
The CdTe quantum of dimercaptosuccinic acid modification is added dropwise in paper base material, paper base material absorbs fixed dimercaptosuccinic acid modification
CdTe quantum after up to dimercaptosuccinic acid modification CdTe quantum paper chip；
(3) serine standard color comparison card is made
The serine of various concentration and the silver ion mixed solution of fixed concentration are prepared, mixed solution is successively added dropwise in mercapto
In the paper chip of the CdTe quantum of base succinic acid modification, observed in ultraviolet camera bellows, various concentration serine and paper chip are anti-
Different colors should be generated, is taken pictures to each paper chip, arrangement obtains serine standard color comparison card；
(4) serine in sample to be tested is detected
By sample and 10-6Mol/L silver ion solution is made mixed solution and the CdTe quantum modified in dimercaptosuccinic acid is added dropwise
In the paper chip of point, paper chip has a color response to sample, and control serine standard color comparison card obtains the dense of serine in sample
Degree.
Preferably, mixed solution is followed successively by 10 in the step (3)-6Mol/L silver ion, 50nM serine and
10-6Mol/L silver ion, 100nM serine and 10-6Mol/L silver ion, 400nM serine and 10-6Mol/L silver ion, 700nM
Serine and 10-6Mol/L silver ion, 1000nM serine and 10-6Mol/L silver ion.
The present invention has the advantages that the present invention is based on paper chips compared with the detection method of existing silver ion and serine
Detect silver ion and serine have preparation it is simple, can rapid field detection, at low cost, fast response time, sensitivity and selection
Property high feature, and paper chip has specific reaction to silver ion and serine, can be used for silver-colored in complex matrices water sample
The detection of ion or serine.
Detailed description of the invention
Fig. 1 is the mechanism schematic diagram that the CdTe quantum of dimercaptosuccinic acid modification is reacted with silver ion；
Fig. 2 is the CdTe quantum transmission electron microscope figure of dimercaptosuccinic acid modification；
Fig. 3 is silver ion standard colorimetric of the CdTe quantum paper chip to various concentration anion of dimercaptosuccinic acid modification
Card；It is 0,100nM, 400nM, 600nM, 800nM, the response diagram of 1000nM that wherein Fig. 3 a~3f, which is followed successively by silver ion solution,；Figure
3A~Fig. 7 F is respectively that Fig. 3 a~3f extracts the silver ion standard color comparison card that color data obtains；
Fig. 4 is serine mark of the CdTe quantum paper chip to the serine solution of various concentration of dimercaptosuccinic acid modification
Quasi- colorimetric card；It is 0,50nM, 100nM, 400nM, 700nM, the reaction of 1000nM that wherein Fig. 4 a~4f, which is followed successively by serine solution,
Figure；Fig. 4 A~Fig. 4 F is respectively that Fig. 4 a~4f extracts the serine standard color comparison card that color data obtains；
Fig. 5 is that the CdTe quantum paper chip of dimercaptosuccinic acid modification detects the color reaction figure of different ions；Fig. 5 A~
5P is followed successively by blank sample, Cd2+、Hg2+、Ca2+、Zn2+、Co2+、Mg2+、Mn2+、Na+、Fe3+、K+、Al3+、Ni2+、Cu2+、Ba2+、Ag+
Response diagram；
Fig. 6 is that the CdTe quantum paper chip of dimercaptosuccinic acid modification detects silver ion figure in complex matrices；Wherein scheme
6A is the silver ion that various concentration is detected in human plasma matrix；Fig. 6 B be in cow's serum matrix detect various concentration silver from
Son；Fig. 6 C is the silver ion that various concentration is detected in South Lake water-based；Fig. 6 D is that detection is different dense in Longjing tea water-based
The silver ion of degree；
Fig. 7 is that the CdTe quantum of dimercaptosuccinic acid modification detects the mechanism schematic diagram of serine.
Specific embodiment
For a better understanding of the invention, invention is described in detail below with reference to drawings and concrete examples.
Complicated for operation to solve existing Silver detection process instruments, the problem of analysis time length, the present invention provides one kind
The method of paper chip the detection silver ion and serine of CdTe quantum based on dimercaptosuccinic acid modification, specifically, by mercapto
The CdTe quantum of base succinic acid modification, which is fixed on paper base material, obtains paper chip, and sample to be detected is added dropwise in paper chip
The concentration of silver ion in sample is judged by the variation of color.It below will be by specific embodiment come to the present invention is based on sulfydryls
The preferred embodiment of the method for the paper chip detection silver ion and serine of the CdTe quantum of succinic acid modification carries out in detail
It is bright.
Embodiment 1
The preparation method of the paper chip of the CdTe quantum of dimercaptosuccinic acid modification, comprising steps of
(1) preparation of the CdTe quantum (TMA-capped CdTe) of dimercaptosuccinic acid modification
Dichloride cadmium (0.1142g, 12.5mM) and dimercaptosuccinic acid (0.0901g, 15mM) are dissolved in 40mL ultrapure water,
PH value of solution is adjusted to 11, then inflated with nitrogen ice bath stirring 20 minutes, be added sodium tellurite (0.0216g, 2.5mM), stirs 15 points
Clock adds sodium borohydride (0.0113g, 7.5mM), and stirring obtains mixed solution in 15 minutes, is finally put into mixed solution instead
It answers in kettle, is reacted 50 minutes in 200 DEG C of baking oven, be cooled to room temperature, obtain 7.10 × 10-5The modification of mol/L dimercaptosuccinic acid
CdTe quantum.Using transmission electron microscope observation, as shown in Fig. 2, it is 3 that transmission electron microscope, which characterizes its particle size,
~5nm.
(2) preparation of the paper chip of the CdTe quantum of dimercaptosuccinic acid modification
It is added dropwise respectively with the CdTe quantum (23.7 μm of ol/L) that liquid-transfering gun draws the modification of 10 μ L dimercaptosuccinic acids in diameter
For the CdTe quantum paper chip that dimercaptosuccinic acid modification is made on the circular filter paper of 5mm, paper chip is placed on 37 DEG C of baking ovens
It dries 4 minutes or so and is done to pico-, be viewed as peony in the ultraviolet camera bellows of 365nm.
Embodiment 2
The method of CdTe quantum paper chip detection silver ion based on dimercaptosuccinic acid modification, comprising steps of
(1) preparation of the CdTe quantum (TMA-capped CdTe) of dimercaptosuccinic acid modification
Dichloride cadmium (0.1142g, 12.5mM) and dimercaptosuccinic acid (0.0901g, 15mM) are dissolved in 40mL ultrapure water,
PH value of solution is adjusted to 11, then inflated with nitrogen ice bath stirring 20 minutes, be added sodium tellurite (0.0216g, 2.5mM), stirs 15 points
Clock adds sodium borohydride (0.0113g, 7.5mM), and stirring obtains mixed solution in 15 minutes, is finally put into mixed solution instead
It answers in kettle, is reacted 50 minutes in 200 DEG C of baking oven, be cooled to room temperature, obtain 7.10 × 10-5The modification of mol/L dimercaptosuccinic acid
CdTe quantum.Using transmission electron microscope observation, as shown in Fig. 2, it is 3 that transmission electron microscope, which characterizes its particle size,
~5nm.
(2) preparation of the paper chip of the CdTe quantum of dimercaptosuccinic acid modification
It is added dropwise respectively with the CdTe quantum (23.7 μm of ol/L) that liquid-transfering gun draws the modification of 10 μ L dimercaptosuccinic acids straight at 3
The CdTe quantum paper chip of 3 dimercaptosuccinic acids modification is made on the circular filter paper that diameter is 5mm, 3 dimercaptosuccinic acids are repaired
The CdTe quantum paper chip of decorations obtains one group of paper chip group by sticking double faced adhesive tape in a paper support, and paper chip group is placed
37 DEG C of baking ovens dry 4 minutes or so to pico- dry, the CdTe quantum paper of observation dimercaptosuccinic acid modification in the ultraviolet camera bellows of 365nm
Chip is peony.
(3) silver ion standard color comparison card is made
Prepare 6 groups of paper chip groups, prepare different silver ion solutions (respectively blank sample, 100nM, 400nM, 600nM,
800nM, 1000nM), the silver ion solution for drawing 10 μ L various concentrations is successively added drop-wise in 6 groups of paper chip groups, and 3 on same group
The concentration of silver ions of a paper chip is identical.Every group of paper chip is different to the response color of silver ion, such as Fig. 3 a~3f institute of Fig. 3
Show, take pictures to every group of paper chip, the color that every group of paper chip generates is imported in Photoshop photo handling software and is extracted
Color data in picture simulates silver ion standard color comparison card based on color data, as shown in Fig. 3 A~3F.
(4) silver ion of sample is detected
Sample is added dropwise in the paper chip for the CdTe quantum that dimercaptosuccinic acid is modified, paper chip has color to ring on sample
It answers, control silver ion standard color comparison card obtains the concentration of silver ion in sample.
The principle of CdTe quantum paper chip detection silver ion based on dimercaptosuccinic acid modification is as shown in Figure 1.
Silver ion can be accurately detected in complex matrices in order to verify the CdTe quantum paper chip of dimercaptosuccinic acid modification,
Carry out complex matrices verification test.Detailed process is that human plasma matrix: preparing 100nM, 400nM respectively in human plasma,
The silver ion of 600nM, 800nM, 1000nM；Cow's serum matrix: preparing 100nM, 400nM, 600nM respectively in cow's serum,
The silver ion of 800nM, 1000nM；South Lake water-based: preparing 100nM, 400nM, 600nM, 800nM in South Lake water respectively,
The silver ion of 1000nM；Longjing tea water-based: preparing 100nM, 400nM, 600nM, 800nM in Dragon Well tea tea respectively,
The silver ion of 1000nM.
The solution for the silver ion for drawing above-mentioned different substrates various concentration with liquid-transfering gun rapidly is added drop-wise to dimercaptosuccinic acid and repairs
In the CdTe quantum paper chip of decorations, as a result as shown in fig. 6, being observed in the ultraviolet camera bellows of 365nm, the complex matrices of various concentration
Middle silver ion color still different from paper chip reaction generation, and as the concentration of silver ion increases, color is by dark red on paper
Discoloration is pink, then becomes pale red, eventually becomes aubergine, reaches in complex matrices Visual retrieval silver ion on paper
Effect.It can thus be seen that dimercaptosuccinic acid modification CdTe quantum paper chip can in complex matrices accurately detection silver from
Son, the interference by matrix are small.
In order to further verify the color response that the CdTe quantum of dimercaptosuccinic acid modification has specificity to silver ion,
Carry out specific test.10 μ L different ions (as shown in connection with fig. 5) are drawn with liquid-transfering gun rapidly to be added dropwise in paper chip, are added dropwise
The paper chip dot of other ions is still in peony, and being added in the paper chip of 10 μ L silver ions becomes purplish red from peony
Color.Illustrate that the CdTe quantum of dimercaptosuccinic acid modification has stronger selectivity to silver ion.
Embodiment 3
The method of CdTe quantum paper chip detection serine based on dimercaptosuccinic acid modification, comprising steps of
(1) preparation of the CdTe quantum (TMA-capped CdTe) of dimercaptosuccinic acid modification
Dichloride cadmium (0.1142g, 12.5mM) and dimercaptosuccinic acid (0.0901g, 15mM) are dissolved in 40mL ultrapure water,
PH value of solution is adjusted to 11, then inflated with nitrogen ice bath stirring 20 minutes, be added sodium tellurite (0.0216g, 2.5mM), stirs 15 points
Clock adds sodium borohydride (0.0113g, 7.5mM), and stirring obtains mixed solution in 15 minutes, is finally put into mixed solution instead
It answers in kettle, is reacted 50 minutes in 200 DEG C of baking oven, be cooled to room temperature, obtain 7.10 × 10-5The modification of mol/L dimercaptosuccinic acid
CdTe quantum.Based on transmission electron microscope observation, as shown in Fig. 2, it is 3 that transmission electron microscope, which characterizes its particle size,
~5nm.
(2) preparation of the paper chip of the CdTe quantum of dimercaptosuccinic acid modification
It is added dropwise respectively with the CdTe quantum (23.7 μm of ol/L) that liquid-transfering gun draws the modification of 10 μ L dimercaptosuccinic acids straight at 3
3 paper chips are made on the circular filter paper that diameter is 5mm, 3 paper chips are obtained one in a paper support by sticking double faced adhesive tape
Paper chip group is placed 37 DEG C of baking ovens and dries 4 minutes or so to doing slightly, is viewed as in the ultraviolet camera bellows of 365nm by group paper chip group
Peony.
(3) serine standard color comparison card is made
It prepares 6 groups of paper chip groups, prepares different serine solution (see solution 1 once to solution 6)
Solution 1:10-6Mol/L silver ion solution；
Solution 2:50nM serine solution+10-6Mol/L silver ion solution；
Solution 3:100nM serine solution+10-6Mol/L silver ion solution；
Solution 4:400nM serine solution+10-6Mol/L silver ion solution；
Solution 5:700nM serine solution+10-6Mol/L silver ion solution；
Solution 6:1000nM serine solution+10-6Mol/L silver ion solution；
The above-mentioned solution 1 of 10 μ L to solution 6 is drawn successively to be added drop-wise in 6 groups of paper chip groups, 3 paper chips on same group
Solution is identical.Every group of paper chip is different to the response color of the serine solution of various concentration, as shown in Fig. 4 b~4f of Fig. 4,
It takes pictures in the ultraviolet camera bellows of 365nm to every group of paper chip, the color that every group of paper chip is generated imports Photoshop picture
The color data in picture is extracted in processing software, serine standard color comparison card is simulated based on color data, such as Fig. 4 A~4F
It is shown.From fig. 4, it can be seen that color becomes pale red from aubergine on paper as serine concentration increases, then become pink
Color eventually becomes peony.Principle such as Fig. 7 of the paper chip detection serine of CdTe quantum based on dimercaptosuccinic acid modification
Shown, principle is, silver ion on serine amino and carboxyl active force it is stronger so that silver ion and quantum dot effect become
Weak, fluorescence restores.
(4) serine in sample to be tested is detected
By sample and 10-6Mol/L silver ion solution is made mixed solution and the CdTe quantum modified in dimercaptosuccinic acid is added dropwise
In the paper chip of point, paper chip has a color response to sample, and control serine standard color comparison card obtains the dense of serine in sample
Degree.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. a kind of paper chip of the CdTe quantum of dimercaptosuccinic acid modification, which is characterized in that including paper base material and be fixed on
The CdTe quantum of dimercaptosuccinic acid modification on paper base material, the CdTe quantum transmitting feux rouges of dimercaptosuccinic acid modification and
Launch wavelength is 620~640nm.
2. the paper chip of the CdTe quantum of dimercaptosuccinic acid modification according to claim 1, which is characterized in that the mercapto
The concentration of the CdTe quantum of base succinic acid modification is 20~25 μm of ol/L, the partial size of the CdTe quantum of dimercaptosuccinic acid modification
For 3~5nm.
3. a kind of method for the CdTe quantum paper chip for preparing dimercaptosuccinic acid modification, which is characterized in that comprising steps of
(1) preparation of the CdTe quantum of dimercaptosuccinic acid modification
Dichloride cadmium and dimercaptosuccinic acid are dissolved in pure water, sequentially add sodium tellurite after pH value of solution is adjusted to 10.5~11.5
And sodium borohydride, it stirs evenly, solution is reacted 40~60 minutes in 180~220 DEG C of baking oven finally, obtains launch wavelength
For the CdTe quantum for the dimercaptosuccinic acid modification of 620~640nm to glow；
(2) preparation of the paper chip of the CdTe quantum of dimercaptosuccinic acid modification
The CdTe quantum of dimercaptosuccinic acid modification is added dropwise in paper base material, paper base material absorbs fixed dimercaptosuccinic acid modification
Up to the CdTe quantum paper chip of dimercaptosuccinic acid modification after CdTe quantum.
4. the method for the CdTe quantum paper chip of dimercaptosuccinic acid modification according to claim 3, which is characterized in that institute
The concentration for stating the CdTe quantum of dimercaptosuccinic acid modification is 20~25 μm of ol/L, the CdTe quantum of dimercaptosuccinic acid modification
Partial size is 3~5nm.
5. the method for the CdTe quantum paper chip of dimercaptosuccinic acid modification according to claim 3, which is characterized in that institute
It states in step (2), the CdTe quantum dripping quantity of dimercaptosuccinic acid modification is 8~10uL, and paper base material is that diameter is 4~6mm's
The paper chip that the CdTe quantum of dimercaptosuccinic acid modification has been added dropwise is placed in 36~39 DEG C of baking oven by circular filter paper, and baking 5~
7 minutes.
6. a kind of method of the paper chip detection silver ion of CdTe quantum based on dimercaptosuccinic acid modification, which is characterized in that
Comprising steps of
(1) preparation of the CdTe quantum of dimercaptosuccinic acid modification
Dichloride cadmium and dimercaptosuccinic acid are dissolved in pure water, sequentially add tellurous acid after pH value of solution is adjusted to 10.5~11.5
Sodium and sodium borohydride, stir evenly, and finally react 40~60 minutes solution in 180~220 DEG C of baking oven, are emitted
The CdTe quantum for the dimercaptosuccinic acid modification to glow that wavelength is 620~640nm；
(2) preparation of the paper chip of the CdTe quantum of dimercaptosuccinic acid modification
The CdTe quantum of dimercaptosuccinic acid modification is added dropwise in paper base material, paper base material absorbs fixed dimercaptosuccinic acid modification
Up to the CdTe quantum paper chip of dimercaptosuccinic acid modification after CdTe quantum；
(3) silver ion standard color comparison card is made
The silver ion solution of various concentration is successively added dropwise in the paper chip of the CdTe quantum of dimercaptosuccinic acid modification,
It is observed in the ultraviolet camera bellows of 365nm, the various concentration silver ion color different from paper chip reaction generation carries out each paper chip
It takes pictures, arrangement obtains silver ion standard color comparison card；
(4) silver ion of sample is detected
Sample is added dropwise in the paper chip for the CdTe quantum that dimercaptosuccinic acid is modified, paper chip has color response to sample,
Control silver ion standard color comparison card obtains the concentration of silver ion in sample.
7. the side of the paper chip detection silver ion of the CdTe quantum according to claim 6 based on dimercaptosuccinic acid modification
Method, which is characterized in that in the step (3), the photo that various concentration silver ion is reacted to generation different colours with paper chip is led
Enter the color data in PaintShop extraction picture, simulates silver ion standard color comparison card using color data.
8. the side of the paper chip detection silver ion of the CdTe quantum according to claim 6 based on dimercaptosuccinic acid modification
Method, which is characterized in that the concentration of silver ion is 0~1000nM in the step (3).
9. a kind of method of the paper chip detection serine of CdTe quantum based on dimercaptosuccinic acid modification, which is characterized in that
Comprising steps of
(1) preparation of the CdTe quantum of dimercaptosuccinic acid modification
Dichloride cadmium and dimercaptosuccinic acid are dissolved in pure water, sequentially add tellurous acid after pH value of solution is adjusted to 10.5~11.5
Sodium and sodium borohydride, stir evenly, and finally react 40~60 minutes solution in 180~220 DEG C of baking oven, are emitted
The CdTe quantum for the dimercaptosuccinic acid modification to glow that wavelength is 620~640nm；
(2) preparation of the paper chip of the CdTe quantum of dimercaptosuccinic acid modification
The CdTe quantum of dimercaptosuccinic acid modification is added dropwise in paper base material, paper base material absorbs fixed dimercaptosuccinic acid modification
Up to the CdTe quantum paper chip of dimercaptosuccinic acid modification after CdTe quantum；
(3) serine standard color comparison card is made
The serine of various concentration and the silver ion mixed solution of fixed concentration are prepared, mixed solution is successively added dropwise in sulfydryl fourth
In the paper chip of the CdTe quantum of diacid modification, observed in ultraviolet camera bellows, various concentration serine reacts production with paper chip
Raw different color, takes pictures to each paper chip, and arrangement obtains serine standard color comparison card；
(4) serine in sample to be tested is detected
By sample and 10-6Mol/L silver ion solution is made mixed solution and the CdTe quantum modified in dimercaptosuccinic acid is added dropwise
In paper chip, paper chip has color response to sample, and control serine standard color comparison card obtains the concentration of serine in sample.
10. the paper chip detection serine of the CdTe quantum according to claim 9 based on dimercaptosuccinic acid modification
Method, mixed solution is followed successively by 10 in the step (3)-6Mol/L silver ion, 50nM serine and 10-6Mol/L silver ion,
100nM serine and 10-6Mol/L silver ion, 400nM serine and 10-6Mol/L silver ion, 700nM serine and 10-6mol/
L silver ion, 1000nM serine and 10-6Mol/L silver ion.
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| CN111504969A (en) * | 2020-04-29 | 2020-08-07 | 中南民族大学 | Preparation method and application of fluorescent sensor based on carbon dot-quantum dot ratio |
| CN112033943A (en) * | 2020-08-17 | 2020-12-04 | 中南民族大学 | Arginine detection method based on quantum dot-copper ion fluorescent substrate sensor |
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| LIAO SEN: "Novel S, N-doped carbon quantum dot-based "off-on" fluorescent sensor for silver ion and cysteine", 《TALANTA》 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111504969A (en) * | 2020-04-29 | 2020-08-07 | 中南民族大学 | Preparation method and application of fluorescent sensor based on carbon dot-quantum dot ratio |
| CN112033943A (en) * | 2020-08-17 | 2020-12-04 | 中南民族大学 | Arginine detection method based on quantum dot-copper ion fluorescent substrate sensor |
| CN112033943B (en) * | 2020-08-17 | 2021-03-30 | 中南民族大学 | Arginine detection method based on quantum dot-copper ion fluorescent substrate sensor |
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