CN109824644A - A method of directly extracting iris aglycone from flower of kudzuvine - Google Patents
A method of directly extracting iris aglycone from flower of kudzuvine Download PDFInfo
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Abstract
The method that the invention discloses a kind of directly to extract iris aglycone from flower of kudzuvine, the following steps are included: Step 1: flower of kudzuvine crushed 60 meshes, flower of kudzuvine powder is weighed to be added in the hydrochloric acid solution that concentration is 0.1-2.0mol/L, it is subsequently placed in thermostat water bath and hydrolyzes 2-6h, hydrolysis temperature is arranged at 50-90 DEG C, and lower sediment thing is centrifuged to obtain after hydrolyzing；Step 2: gained sediment distillation is washed to neutrality, the ethanol solution of 200mL85% is added, after extracting 2h at 60 DEG C, lower sediment is abandoned in centrifugation, obtains supernatant leaching liquor；And upper extraction clear liquid is centrifuged to obtain supernatant as 4 DEG C of cold heavy 4h；Step 3: gained supernatant is concentrated under reduced pressure into 10-20mL, then gained concentrate addition 10 times of amounts, 70 DEG C of distilled water are stirred and evenly mixed, cold heavy 12h, centrifuging and taking precipitating are freeze-dried 36h, obtain extraction powder, i.e. iris aglycone at 4 DEG C after being cooled to room temperature.The present invention directly obtains iris aglycone from flower of kudzuvine, and without the extraction step of iridin, the recovery rate of simple process, and iris aglycone is high.
Description
Technical field
The present invention relates to plants ' medicinal component extractive technique fields, particularly relate to one kind and directly extract from flower of kudzuvine
The method of iris aglycone.
Background technique
Isoflavones (isoflavoen) is a kind of phytoestrogen (phytoestrongen), has oestrogen-like hormone sample or anti-
Estrogen action, referred to as natural selective estrogen receptor modulators play important protective effect to human health.Different Huang
Ketone poisonous side effect of medicine is small, securely and reliably, has multiple biological activities, application prospect is extensive.However, the separation of isoflavones is pure
Chemical industry sequence is loaded down with trivial details, time and effort consuming, and usually needs a variety of toxic organic solvents, not only increases the cost of drug, and give
Environment brings more or less pollution, limits the development and application of osajin drug to a certain extent.
Isoflavone compound is distributed widely in plant kingdom, but the content of isoflavones is very low.Iris aglycone is as different Huang
One kind of ketone, the active ingredient of the iridin extracted from flower of kudzuvine are its aglycon, different containing a methoxyl group and three hydroxyls
Flavones has strong anti-oxidative activity.Flower of kudzuvine extracts isoflavones effective component from flower of kudzuvine and uses in Natural Resources in China very abundant
It is an effective measure for efficiently using natural resources in new drug development.
The present invention discloses a kind of iris aglycone new preparation process, iris aglycone is directly acquired from flower of kudzuvine, without iridin
Extraction step, for making in flower of kudzuvine active increase of isoflavones that there is far-reaching influence.
Summary of the invention
To solve the problems mentioned above in the background art, the purpose of the present invention is to provide one kind directly to mention from flower of kudzuvine
The method for taking iris aglycone.
To achieve the above object, the technical scheme adopted by the invention is as follows:
A method of directly extracting iris aglycone from flower of kudzuvine, comprising the following steps:
Step 1: flower of kudzuvine crushed 60 meshes, weighs flower of kudzuvine powder and be added to the hydrochloric acid that concentration is 0.1-2.0mol/L
Solution is subsequently placed in thermostat water bath that is, in the aqueous solution of HCl and hydrolyzes 2-6h, and hydrolysis temperature is arranged at 50-90 DEG C, to water
Lower sediment thing is centrifuged to obtain after solution；
Step 2: the distillation of step 1 gained sediment is washed to neutrality, the ethanol solution of 200mL85% is added,
After extracting 2h at 60 DEG C, lower sediment is abandoned in centrifugation, obtains supernatant leaching liquor；And upper extraction clear liquid is placed at 4 DEG C cooling precipitating
4h is centrifuged to obtain supernatant；
Step 3: supernatant obtained by step 2 is concentrated under reduced pressure into 10-20mL, 10 times of amounts then are added in gained concentrate
70 DEG C of distilled water, are cooled to room temperature after stirring and evenly mixing, and are subsequently placed at 4 DEG C cooling precipitating 12h, centrifuging and taking precipitating, freeze-drying
36h obtains extracting powder, i.e. iris aglycone.
In above-mentioned technical proposal, in step 1, the concentration of hydrochloric acid solution is 1.5mol/L, and hydrolysis temperature is 90 DEG C, hydrolysis
Acidolysis is 6h.
In above-mentioned technical proposal, in step 1, amount/mmol ratio of the substance of the quality/g and HCl of flower of kudzuvine powder is
1/40(g/ml)。
In above-mentioned technical proposal, in step 1, the quality of flower of kudzuvine powder is 10g, and the amount of the substance of HCl is 400mmol.
Compared with prior art, the beneficial effects of the present invention are:
1, iris aglycone is directly acquired from flower of kudzuvine, without the extraction step of iridin, simple process；Unit mass simultaneously
The resulting iris aglycone of flower of kudzuvine is greater than the resulting iridin of unit mass flower of kudzuvine, and iris aglycone functional activity is better than iridin.
2, the present invention improves the recovery rate that iris aglycone is directly obtained from flower of kudzuvine, and recovery rate is up to 1.89 ±
0.09%.
Detailed description of the invention
Fig. 1 is influence of the different acidolysis temperatures to iris aglycone yield in the present invention；
Fig. 2 is influence of the different acidolysis times to iris aglycone yield in the present invention；
Fig. 3 is influence of the different concentration of hydrochloric acid to iris aglycone yield in the present invention；
Fig. 4 is the influence that different feed liquid compares iris aglycone yield in the present invention；
Fig. 5 is extract infrared scan map in embodiment；
Fig. 6 a is the LC-MC parent ion peak of iris aglycone standard items in embodiment；Fig. 6 b is the LC- of extract in the present invention
MC parent ion peak；
Fig. 7 a is the LC-MC daughter ion peak of iris aglycone standard items in embodiment；Fig. 7 b is the LC- of extract in the present invention
MC daughter ion peak；
Fig. 8 a is the LC-MC characteristic ion of iris aglycone standard items in embodiment；Fig. 8 b is the LC- of extract in the present invention
MC characteristic ion；
Fig. 9 is the reducing power of iridin, iris aglycone in embodiment；
Figure 10 is the ability of iridin in embodiment, iris aglycone removing DPPH free radical；
Figure 11 is the ability of iridin in embodiment, iris aglycone removing ABTS+ free radical；
Figure 12 be in embodiment iridin and iris aglycone to the inhibitory effect of tyrosinase；
Figure 13 is the HPLC map of iris aglycone extract in embodiment.
Specific embodiment
To be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, below with reference to
The drawings and specific embodiments, how the present invention is further explained implements.
The present invention provides a kind of method that iris aglycone is directly extracted from flower of kudzuvine, by acidifying water solution directly from flower of kudzuvine
In directly extract iris aglycone, without the intermediate extraction step through iridin in flower of kudzuvine.
The present invention provides a kind of method that iris aglycone is directly extracted from flower of kudzuvine, comprising the following steps:
Step 1: flower of kudzuvine crushed 60 meshes, the hydrochloric acid solution that flower of kudzuvine powder is added to 0.1-2.0mol/L is weighed
In (aqueous solution of HCl), be subsequently placed in thermostat water bath and hydrolyze 2-6h, hydrolysis temperature be arranged at 50-90 DEG C, after hydrolyzing from
Gains in depth of comprehension lower sediment thing；
Step 2: the distillation of step 1 gained sediment is washed to neutrality, the ethanol solution of 200mL85% is added,
After extracting 2h at 60 DEG C, lower sediment is abandoned in centrifugation, obtains supernatant leaching liquor, i.e. alcohol extract；And by supernatant as 4 DEG C,
Cooling precipitating 4h, is centrifuged to obtain supernatant；
Step 3: supernatant obtained by step 2 is concentrated under reduced pressure into 10-20mL, 10 times of amounts then are added in gained concentrate
70 DEG C of distilled water, are cooled to room temperature after mixing evenly, are subsequently placed at 4 DEG C cold heavy 12h (since iris aglycone polarity is small, hydrophilic
Property it is poor, can be used that water is heavy to remove hydroaropic substance), centrifuging and taking precipitating is freeze-dried 36h, obtains extracting powder, i.e. iridin
Member.
Using the yield of HPLC measurement iris aglycone in the present invention:
85% alcohol extract of gained in step 2 is settled to 250mL, after 0.45 μm of filtering with microporous membrane, is surveyed through HPLC
Determine yield.
In formula: C is the concentration of iris aglycone in leaching liquor, mg/mL；M is flower of kudzuvine quality, mg.
Wherein, HPLC testing conditions: chromatographic column: HypersilBDS (4.6mm × 250mm, 5 μm)；Mobile phase: methanol-water
(40:60)；Detection wavelength: 265nm；Flow velocity: 1mL/min；Column temperature: 30 DEG C；Sample volume: 10 μ L.
(1) present invention studies the influence factor extracted to iris aglycone by single_factor method, finds acidifying water solution from Pueraria lobota
Spend extract iris aglycone during, concentration of hydrochloric acid solution 1mol/L, hydrolysis temperature be 90 DEG C, hydrolysis acidolysis be 6h and
The quality of flower of kudzuvine powder and the material ratio of hydrochloric acid solution influence the recovery rate of iris aglycone.It is specific as follows:
One, hydrolysis temperature influences
5 parts of flower of kudzuvine raw material, each 10g of every part of powder are accurately weighed, the hydrochloric acid solution of 1mol/L is added into every part of powder
200mL, respectively under conditions of temperature is 50 DEG C, 60 DEG C, 70 DEG C, 80 DEG C, 90 DEG C, water bath with thermostatic control, the acidolysis time is 5h, measurement
The yield variation with temperature of iris aglycone, result are as shown in Figure 1.
As shown in Figure 1, the yield variation with temperature of iris aglycone is very significant (p < 0.05), and yield is with acidolysis temperature
The increase of degree steps up.When acidolysis temperature is lower (50 DEG C, 60 DEG C), iris aglycone yield is less than 0.5%；When temperature is from 60
It DEG C improves to during 90 DEG C, iris aglycone yield is significantly improved, and when rising to 80 DEG C, there are about 1.5%；When temperature reaches 90
DEG C when, most of iridin is all hydrolyzed to iris aglycone, and the yield of iris aglycone can achieve 1.8% or more.Meanwhile extracting temperature
Some impurity can be made sufficiently to decompose when spending higher, or be dissolved in hot water, to improve the purity of iris aglycone extract.
Therefore select 90 DEG C for best acidolysis temperature.
Two, the acidolysis time
5 parts of flower of kudzuvine raw material, each 10g of every part of powder are accurately weighed, the hydrochloric acid solution of 1mol/L is added into every part of powder
200mL is placed in 80 DEG C of water-bath, respectively constant temperature acidolysis 2h, 3h, 4h, 5h, 6h, when measuring iris aglycone yield with acidolysis
Between variation, result is as shown in Figure 2.
As shown in Figure 2, influence of the acidolysis time to iris aglycone yield has conspicuousness (p < 0.05), iris aglycone
Rate is with the increase of acidolysis time and in the trend stepped up.When the acidolysis time is shorter (2h), iris aglycone yield less than 1%,
When the acidolysis time extending to 6h, iris aglycone yield rises to 1.76%.So selecting 6h for the best acidolysis time.
Three, concentration of hydrochloric acid solution
Accurately weigh 5 parts of flower of kudzuvine raw material, each 10g of every part of powder, be separately added into concentration be 0.1mol/L, 0.5mol/L,
The hydrochloric acid solution 200mL of 1mol/L, 1.5mol/L, 2mol/L, are placed in 80 DEG C of water-bath, constant temperature acidolysis 5h, measure iris
For aglycon yield with the variation of concentration of hydrochloric acid, result is as shown in Figure 3.
From the figure 3, it may be seen that influence of the concentration of hydrochloric acid to the yield of iris aglycone is very significant (p < 0.05).With concentration of hydrochloric acid
Raising, the yield of iris aglycone is in rising trend.When concentration of hydrochloric acid increases to 1.5mol/L, iris aglycone yield is
1.77%, the increased rate of yield obviously slows down later.Although under 2mol/L concentration of hydrochloric acid, iris aglycone yield still some
Perhaps increase, but under high concentration of hydrochloric acid long duration of action, iris aglycone can be decomposed, and lose activity, and for cost and safety
Property consider, select 1.5mol/L for best concentration of hydrochloric acid.
Four, solid-liquid ratio
Accurately weigh flower of kudzuvine powder 10g, totally 5 parts, be separately added into concentration be 1mol/L hydrochloric acid solution 100mL, 200mL,
300mL, 400mL, 500mL are placed in 80 DEG C of water-bath, constant temperature acidolysis 5h, measure the variation of iris aglycone yield, result
As shown in Figure 4.
It will be appreciated from fig. 6 that iris aglycone yield becomes with solid-liquid ratio (flower of kudzuvine quality: the amount of HCl substance, g/mmol) in irregular
Change, when solid-liquid ratio is 1:10, yield is only 1.18%；As 1:20, yield is increased；When solid-liquid ratio is 1:30, obtain
Rate is fallen after rise；When being wherein 1:40 with solid-liquid ratio, yield highest is 1.51%；When solid-liquid ratio is 1:50, yield 1.5%.
Therefore, it is in cost consideration, selects 1:40 as optimum extraction solid-liquid ratio.
Specific embodiment
The method that the present embodiment provides a kind of directly to extract iris aglycone from flower of kudzuvine, comprising the following steps:
Step 1: flower of kudzuvine crushed 60 meshes, the hydrochloric acid solution that 10g flower of kudzuvine powder is added to 1.5mol/L is weighed
It in 267mL (1/40), is subsequently placed in thermostat water bath and hydrolyzes 6h, hydrolysis temperature is arranged at 90 DEG C, is centrifuged after hydrolyzing
Layer sediment；
Step 2: the distillation of step 1 gained sediment is washed to neutrality, the ethanol solution of 200mL85% is added,
After extracting 2h at 60 DEG C, lower sediment is abandoned in centrifugation, obtains supernatant leaching liquor, i.e. alcohol extract；And by supernatant as 4 DEG C,
Cold heavy 4h, is centrifuged to obtain supernatant；
Step 3: supernatant obtained by step 2 is concentrated under reduced pressure into 20mL, 10 times of amounts 70 then are added in gained concentrate
DEG C distilled water, is cooled to room temperature after mixing evenly, is subsequently placed at 4 DEG C cold heavy 12h (since iris aglycone polarity is small, hydrophily
The heavy removing hydroaropic substance of water can be used in difference), centrifuging and taking precipitating is freeze-dried 36h, obtains extracting powder, i.e. iris aglycone.
In the present embodiment, 85% alcohol extract of gained in step 2 is settled to 250mL, 0.45 μm of filtering with microporous membrane
It afterwards, is 1.89 ± 0.09% through HPLC measurement iris aglycone yield.
In the prior art, the preparation method of iridin is general are as follows: takes flower of kudzuvine 100g to be placed in three-necked bottle, solvent 1:1 is added
(v/v) n- n-butanol phase/water 1000mL, refluxing extraction 1.5h under conditions of leaching temperature is 70 DEG C.It is quiet after leaching liquid filtering
Horizontalization weighing apparatus, separates n-butanol phase.By the n-butanol being obtained by filtration mutually with rotary evaporation to 100mL, it is concentrated and mutually places at room temperature
12h, obtains the crystallisation by cooling of isoflavones, and filtration drying obtains powder.Dry powder uses recrystallizing methanol.Solid content is obtained,
Naturally dry is to get iridin.Iridin, yield 0.62% is made using the prior art in the present embodiment.
In the present embodiment, iris aglycone performance test:
(1) Structural Identification
1.1 chromogenic reaction qualification results
To progress ferric chloride reaction extract obtained in embodiment and hydrochloric acid-magnesium powder reaction assay, reacting phenomenon and knot
Fruit is shown in Table 1.
1 chromogenic reaction phenomenon of table and result
As shown in table 1, occur red when ferric chloride reaction, illustrate that there are flavone compounds in extract；Hydrochloric acid-magnesium
There is yellow green when powder reaction assay, illustrates that there are phenolic hydroxyl groups in extract；The result shows that there are iris aglycones in extract
Functional group.
1.2 infrared scan
Pelletized powder samples will be extracted and carry out infrared test, infrared scan map is as shown in Figure 5: 3281cm-1 is the flexible vibration of O-H
Dynamic characteristic absorption；2924cm-1 is the absorption of-CH3；1646cm-1 is the characteristic absorption for being conjugated ketone carbonyl stretching vibration；
1616cm-1,1565cm-1,1514cm-1 and 1465cm-1 are the characteristic absorption of aromatic ring frame vibration；1062cm-1 is C-O-C
Absorption.The result shows that there are the functional groups of iris aglycone in extract.
1.3HPLC-MS qualification result
ShuPan etc. analyzes flavones and liposoluble ingredient in iris, and experimental method is electrospray multi-stage mass method, it is determined that
The fragment ion of iris aglycone is 286,229,168 and 159.
Measure iris aglycone reference substance and extract by LC-MS, obtain corresponding positive ion mass spectrum figure, Fig. 6 a and
The parent ion of the iris aglycone reference substance and extract that are provided respectively in Fig. 6 b be m/z301.00 (M+H)+, determine iridin
First molecular weight is 300.00.
As illustrated in figs. 7 a and 7b, the characteristic ion of iris aglycone reference substance and extract to be m/z167.95 (M+H)+
With m/z286.00 (M+H)+.M/z167.95 (M+H)+and m/z286.00 (M+H)+being respectively m/z301.00 (M+H)+lose one
The quasi-molecular ions that the neutral fragment of a mass number 133.05 and 15 generates.
As shown in figs. 8 a and 8b, iris aglycone reference substance and extract through HPLC its corresponding retention time daughter ion
Peak, display result are completely the same, it was demonstrated that the main component of the extract is iris aglycone really.
(2) Function Identification
The reducing power measurement result analysis of 2.1 iridins, iris aglycone
Iridin, the kite of 0.005mg/mL, 0.025mg/mL, 0.05mg/mL, 0.1mg/mL, 0.15mg/mL are measured respectively
The size of tail aglycon and vitamin C reducing power.Wherein iridin, iris aglycone are that the present embodiment is made, and vitamin C can be in market
It purchases；As shown in Figure 9, with the raising of three kinds of material concentrations, their reducing power is in rising trend, and conspicuousness is related
(p<0.05).In the concentration range of 0mg/mL~0.15mg/mL, reducing power size order is: vitamin C > iris aglycone > kite
Tail glycosides.
2.2 iridins, iris aglycone analyze the Scavenging activity measurement result of DPPH
The size of hydroxyl hydrogen supply capacity will affect the scavenging effect of DPPH free radical in antioxidant molecule structure.In room
Under temperature, DPPH free radical be usually it is stable, after it is dissolved in ethyl alcohol, color is dark purple, can be at wavelength 517nm
Obtained the maximum absorption is detected.After H proton or son are in conjunction with DPPH, the color of solution can be gradually become shallower as, what solution shoaled
Degree number direct proportionality (Hanetal2012 of proton or electronics in conjunction with DPPH；Li Xiongli 2012).So in order to
The size of oxidation resistance is embodied, the front and back light absorption value that sample is added in DPPH solution can be compared by we, according to it
Difference is evaluated come the ability for providing Hydrogen Proton to substance.
Measure (0.01mg/mL, 0.05mg/mL, 0.1mg/mL, 0.15mg/mL, 0.2mg/mL) iris of various concentration
Glycosides, iris aglycone remove the ability of DPPH free radical；Wherein iridin, iris aglycone are that the present embodiment is made, and vitamin C can be in
It purchases in market.The ability that iridin, iris aglycone and positive control vitamin C remove DPPH free radical is as shown in Figure 10.
As shown in Figure 10, the capacity of water of three kinds of substances removing DPPH free radicals is as material concentration increases, and increases.
When ascorbic concentration is less than 0.01mg/mL, to the clearance rate size of DPPH free radical as the increase of concentration mentions rapidly
It is high；And when the concentration of iridin, iris aglycone is greater than 0.01mg/mL, the two is just bright to the difference of DPPH free radical scavenging activity
It is aobvious.Iridin, iris aglycone and ascorbic IC50 value are respectively 69.36 μ g/mL, 46.34 μ g/mL and 5.65 μ g/mL, clearly
Except the power of DPPH free radical ability shows as vitamin C > iris aglycone > iridin.Thus, in order to from flower of kudzuvine without iris
The extraction step of glycosides, and directly acquire the technology path of iris aglycone with scientific and feasibility, this study for
Make active increase of isoflavones in flower of kudzuvine that there is far-reaching influence.
The Scavenging activity interpretation of result of 2.3 iridins, iris aglycone to ABTS free radical
Removing ABTS+ free radical ability is a kind of currently used finger for judging the total antioxidant capacity size of substance
Mark.The ABTS being oxidized can become ABTS radical cation, be sufficiently stable, but after it is soluble in the aqueous phase system, molten
Liquid color shows as blue-green, can detect obtained the maximum absorption at wavelength 734nm.The test is exactly to utilize anti-oxidant work
What property can be calculated ABTS+ radical reduction degree by substance.So being caused when ABTS+ free radical is reduced
Solution colour the case where fading, the phenomenon that being exactly the experiment, expresses, and is embodied in light absorption value reduction, and light absorption value becomes smaller, under
Drop degree shows that more greatly the ability of substance removing ABTS+ free radical is stronger.
It is respectively 0.01mg/mL, 0.05mg/mL, 0.1mg/mL, 0.15mg/mL, 0.2mg/ with 95% ethyl alcohol compound concentration
Iridin, iris aglycone and the vitamin c solution of mL；Wherein iridin, iris aglycone are that the present embodiment is made, and vitamin C can
It is purchased in market.The ability that iridin, iris aglycone and positive control vitamin C remove ABTS+ free radical is as shown in figure 11.
As shown in Figure 11, when concentration range is between 0mg/mL~0.2mg/mL, iridin, iris aglycone and vitamin
C is in rising trend to the clearance rate of ABTS+ free radical.Iridin, iris aglycone and ascorbic IC50 value are respectively
135.31 μ g/mL, 45.59 μ g/mL and 44.62 μ g/mL.When material concentration is less than 0.05mg/mL, ABTS+ free radical is removed
Capacity of water is iris aglycone > vitamin C > iridin；But when concentration is greater than 0.05mg/mL, vitamin C removes ABTS+ certainly
Iridin and iris aglycone are apparently higher than by the ability of base, and the ability of iris aglycone is better than iridin.This result shows that, kite
Tail glycosides and iris aglycone all have oxidation resistance, are capable of providing Hydrogen Proton and terminate free chain reaction.
The influence of 2.4 iridins, iris aglycone to tyrosinase inhibition rate
With phosphate buffer, propylene glycol and ethyl acetate configuration concentration 1g/L, 1.5g/L, 2g/L, 2.5g/L, 3g/L
Iridin, iris aglycone and vitamin c solution measure their inhibition to tyrosinase using vitamin C as positive control
Rate；Wherein iridin, iris aglycone are that the present embodiment is made, and vitamin C can be purchased in market.
As shown in Figure 12, with the increase of iridin and iris aglycone concentration, the inhibiting rate to tyrosinase is not in
The trend being stepped up, i.e. the partial function factor can generate inhibiting effect to tyrosinase when too high levels, this is also referred to as
Two-way function.Iridin, iris aglycone and ascorbic IC50 value are respectively 2.62mg/mL, 1.39mg/mL and 0.51mg/
mL.Iris aglycone improves the inhibiting rate of tyrosinase with the raising of concentration in this test, but the meeting after rising to highest point
It falls after rise, traces sth. to its source, the concentration due to when not reaching peak value, improving iris aglycone can enhance it to tyrosinase
Inhibitory effect, and in 3mg/mL, iris aglycone declines the inhibiting rate of tyrosinase, then illustrates that it, with two-way function, is led
Inhibiting rate curve is caused to fluctuate, inhibiting rate is declined.Although and iridin is in concentration to the inhibiting rate of tyrosinase
Occurs wave phenomenon when 2mg/mL, but whole in rising trend, the reason is that since the concentration of iridin in test not up to swashs
The concentration of tyrosinase living, so there is not downward trend in 3mg/mL.By the ascorbic inhibiting rate of positive reference substance with
Iridin, iris aglycone compare, suppression of the iris aglycone in intermediate concentration 2mg/mL and 2.5mg/mL, to tyrosinase
Effect processed can achieve 95% or more, and effect is similar to vitamin C.And at any concentration, ascorbic inhibitory effect all compares
The inhibitory effect of iridin will be got well.For iridin only when concentration is 1mg/mL, inhibitory effect is stronger than iris aglycone, this is because
The inhibiting rate measurement of iris aglycone low concentration is not directed in test, and in low concentration, iris aglycone inhibiting effect exists certain
Fluctuation, thus occur moiety concentrations inhibiting effect be less than iridin the case where；With the increase of sample concentration, iris aglycone
Inhibiting rate be above iridin, also comply with iris aglycone activity be higher than iridin conclusion.
It is tested by above-mentioned Function Identification it is found that iris aglycone is in reducing power, the ability of removing DPPH free radical, removing ABTS
The ability of+free radical and iridin is above to the inhibiting rate etc. of tyrosinase, illustrates that iris aglycone activity is higher than iris
Glycosides.
In conclusion method provided by through the invention directly prepares iris aglycone, the resulting kite of unit mass flower of kudzuvine
Tail aglycon is greater than the resulting iridin of unit mass flower of kudzuvine, and iris aglycone functional activity is better than iridin.
In the present invention, iris aglycone Purity is carried out using HPLC:
Purity experiment description: iris aglycone obtained by the present invention is taken, obtains kite using high performance liquid chromatography (HPLC)
The HPLC map of tail aglycon extract measures the peak area A of iris aglycone titer, draws peak area A (mv*min) with sample
The curve of product concentration (mg/mL) variation and variation.Obtain regression equation: Y=3 × 107X-76045, R2=0.9997；Linear model
It encloses: 21.2mg/L~106mg/L；Sample purity is calculated according to regression equation Y=3 × 107X-76045 of drafting, extract is pure
Degree is 55-90%.
It can be observed from fig. 13 that there is 2 impurity peaks, root in the methanol solution of iris aglycone extract obtained by the present embodiment
Sample purity is calculated according to regression equation Y=3 × 107X-76045 of drafting, and extract purity is that 88.78% (retention time is
27.890min), the retention time of impurity is respectively 6.083min and 18.782min.
Finally, it is stated that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although referring to compared with
Good embodiment describes the invention in detail, those skilled in the art should understand that, it can be to skill of the invention
Art scheme is modified or replaced equivalently, and without departing from the objective and range of technical solution of the present invention, should all be covered at this
In the scope of the claims of invention.
Claims (4)
1. a kind of method for directly extracting iris aglycone from flower of kudzuvine, which comprises the following steps:
Step 1: flower of kudzuvine crushed 60 meshes, weighs flower of kudzuvine powder and is added to the hydrochloric acid solution that concentration is 0.1-2.0mol/L,
I.e. in the aqueous solution of HCl, be subsequently placed in thermostat water bath and hydrolyze 2-6h, hydrolysis temperature be arranged at 50-90 DEG C, after hydrolyzing from
Gains in depth of comprehension lower sediment thing；
Step 2: the distillation of step 1 gained sediment is washed to neutrality, the ethanol solution of 200mL85% is added, 60
After extracting 2h at DEG C, lower sediment is abandoned in centrifugation, obtains supernatant leaching liquor；And supernatant leaching liquor is placed at 4 DEG C cooling precipitating 4h, from
Gains in depth of comprehension supernatant；
Step 3: supernatant obtained by step 2 is concentrated under reduced pressure into 10-20mL, gained concentrate is then added 10 times and measures 70 DEG C
Distilled water is cooled to room temperature after stirring and evenly mixing, and is subsequently placed at 4 DEG C cooling precipitating 12h, and centrifuging and taking precipitating is freeze-dried 36h,
It obtains extracting powder, i.e. iris aglycone.
2. a kind of method for directly extracting iris aglycone from flower of kudzuvine according to claim 1, which is characterized in that step 1
In, the concentration of hydrochloric acid solution is 1.5mol/L, and hydrolysis temperature is 90 DEG C, and hydrolysis acidolysis is 6h.
3. a kind of method for directly extracting iris aglycone from flower of kudzuvine according to claim 1, which is characterized in that step 1
In, amount/mmol ratio of the substance of the quality/g and HCl of flower of kudzuvine powder is 1/40.
4. a kind of method for directly extracting iris aglycone from flower of kudzuvine according to claim 1 or 3, which is characterized in that step
In rapid one, the quality of flower of kudzuvine powder is 10g, and the amount of the substance of HCl is 400mmol.
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