CN109813694A - A kind of Portable visual fluorescence detection device - Google Patents
A kind of Portable visual fluorescence detection device Download PDFInfo
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- CN109813694A CN109813694A CN201910201692.9A CN201910201692A CN109813694A CN 109813694 A CN109813694 A CN 109813694A CN 201910201692 A CN201910201692 A CN 201910201692A CN 109813694 A CN109813694 A CN 109813694A
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- unstressed configuration
- background board
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- positioning base
- light source
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
A kind of Portable visual fluorescence detection device, belongs to sample analysis field.The device is connected by a hinge by lid section and box portion.Unstressed configuration background board is equipped in cabinet, unstressed configuration positioning base is equipped with before unstressed configuration background board, sample bottle can be placed on unstressed configuration positioning base, unstressed configuration background board opposite is equipped with image collector, excitation light source is equipped with above image collector, it is equipped with power interface and data transmission interface in body side, is additionally provided with handle on the outside of cabinet.A kind of Portable visual fluorescence detection device of the present invention, have many advantages, such as easy to operate, safety and stability, easy to remove, reliable performance, it is cheap, occupy little space.
Description
Technical field
The present invention relates to a kind of Portable visual fluorescence detection devices, belong to sample analysis field.
Background technique
Fluorescence detection device is used to detect the various samples that can produce fluorescence after exciting with light, is widely used in various
Field: in sample analysis field, it is usually used in detecting the samples such as protein, nucleotide;In drug is produced and studied, it to be used for hormone
The drug qualities inspection such as alkaloid, vitamin;In the chemical industries such as dyestuff coating rubber, petroleum, for various fluorescent materials,
Fluorescence indicator and additive measurement, identify the kind of crude oil and rubber product;It can be used for measuring in textile chemistry fiber
Different types of raw material such as wool, silk staple fibre, cotton synthetic fibers, and can check final product quality.In grain and oil, vegetable
Dish, food department can be used for checking toxin (such as aflatoxins), food additives, the quality of rotten veterinary antibiotics etc.;?
The true and false that the departments such as geology, archaeology can play and find various minerals, differentiate historical relic fossil;It can be checked in public security department and refer to that trace is surveyed
Fixed, secret writing writing, counterfeit money etc..
Traditional ultraviolet fluorescence analysis instrument space is small, and primary observable sample size is limited, and weight is big, not Portable belt,
And photosampling real-time, in situ can not be carried out to sample, it is unfavorable for post analysis, processing and archive;With external camera or mobile phone
Although etc. means also available sample fluorescence photo, need to photo post-processing;In addition, this external photograph
Method can all influence the figure finally obtained with the transformation of shooting angle, the power of light, sample background variation, change in location etc.
Tablet quality can improve the above problem by technological means such as image procossings, but time-consuming, labour-consuming, and to a collection of similar sample
Picture be difficult to ensure identical illuminance, clarity, equal angular etc..In addition, fluorescence analysis is 190 usually using wavelength
The excitation light source of~400 nanometers of ultraviolet bands, this kind of light source to easily causing superficial injury, especially eyes and baring skin,
It is impaired to will lead to temporary or permanent vision when serious, or even blindness.Therefore traditional ultraviolet fluorescence analysis instrument, which exists, improves
Necessity.
Summary of the invention
In order to solve above-mentioned problems of the prior art, the present invention provides a kind of Portable visual fluorescence detections
Device, device shooting sample size is more, and obtained shooting sample quality is stablized.
A kind of Portable visual fluorescence detection device includes with lower component: lock, case lid, hinge, cabinet, handle, electricity
Source interface, data transmission interface, image collector, excitation light source, unstressed configuration background board, unstressed configuration positioning base.The device by
Lid section and box portion are connected by a hinge.The outer surface of one side of lid section is equipped with lock.Unstressed configuration
Background board one side is fitted in the inner surface that cabinet is connected with hinge side completely, is equipped with unstressed configuration below unstressed configuration background board and positions bottom
Seat, the side are bonded completely with unstressed configuration background board another side, and unstressed configuration positioning base bottom surface is fitted in tank floor, unstressed configuration
Positioning base is being equipped with several location holes with plane perpendicular direction, and location hole aperture is more than or equal to the outer diameter of sample bottle, and without glimmering
The opposite body side inner surface center of light background board is equipped with image collector, is equipped with strip by proximal edge far from bottom surface
Excitation light source, excitation light source is parallel with tank floor, and handle is equipped on the outside of side equipped with excitation light source, and is equipped with exciting light
Power interface and data transmission interface are equipped on the inside of a vertical body side of the body side in source.
It is connect inside the power interface and image collector and excitation light source, data transmission interface and image collector
Inside connection.
The excitation light source is the row's formula or tubular type excitation light source that length is more than or equal to unstressed configuration positioning base length, wave
A length of 190~400nm.
The length of the unstressed configuration background board is greater than or equal to the length of unstressed configuration positioning base, and the material of the two is
Polytetrafluoroethylene (PTFE).
Power supply is accessed when work, data transmission interface connects data equipment, and the sample bottle equipped with sample is fixed on fluorescence
The positioning hole of positioning base, covers case lid, and locking lock opens the switch of excitation light source on cabinet, carries out the fluorescence of sample
Detection.
A kind of Portable visual fluorescence detection device of the present invention has easy to operate, safety and stability, convenient for shifting
Dynamic, reliable performance, cheap, the advantages that occupying little space;It can clearly, comprehensively be observed when carrying out batch sample detection
Sample and it is carried out in real time obtain batch sample fluorescence take pictures, the shooting angle of acquired picture, size, Color-sensitive
Degree, stray light background etc. are consistent, and operator is allowed to improve working efficiency, improve the accuracy of experimental result, and conveniently quantitative pair
When post analysis processing and archive, improve work efficiency the accuracy with experimental result;The device can make light source simultaneously
Closing in the case, prevents the skin and eyes of light leakage injury tester;Finally, tester can be according to requiring to image collection
The lens parameters of device are set to meet different shooting demands.
Detailed description of the invention
Fig. 1 is the structural diagram of the present invention.
Fig. 2 is auxiliary view of the invention.
Fig. 3 is the colour developing photo under the different CdSe quantum dot room lighting light that the present invention obtains.
Fig. 4 is the fluorescence photo for the different CdSe quantum dots that the present invention obtains.
If Fig. 1 is latched, 2. case lids, 3. hinges, 4. cabinets, 5. handles, 6. power interfaces, 7. data transmission interfaces, 8. figures
As collector, 9. excitation light sources, 10. unstressed configuration background boards, 11. unstressed configuration positioning bases, 12. sample bottles.
Specific embodiment
Portable visual fluorescence detection device is described further combined with specific embodiments below.
Embodiment 1
A kind of Portable visual fluorescence detection device is formed by connecting by lid section and box portion by hinge 3.Case
The outer surface of one side of cover is equipped with lock 1.10 one side of unstressed configuration background board is fitted in cabinet completely and is connected with 3 side of hinge
The inner surface in face, 11 side of unstressed configuration positioning base are bonded completely with 10 another side of unstressed configuration background board, unstressed configuration positioning base
11 bottom surfaces are fitted in tank floor, and unstressed configuration positioning base 11 is being equipped with several location holes, location hole hole with plane perpendicular direction
Diameter is more than or equal to the outer diameter of sample bottle 12, and the body side inner surface center opposite with unstressed configuration background board 10 is equipped with image
Excitation light source 9 is equipped with above collector 8, image collector 8, excitation light source 9 is parallel with tank floor, equipped with excitation light source 9
It is equipped with handle 5 on the outside of side, power interface 6 and data transmission interface 7 are equipped on the inside of a body side being connected with the side.
Colour developing photo under the different CdSe quantum dot room lighting light of embodiment 2
The present invention is used to shoot colour developing result of the fluorescence quantum CdSe quantum dot indoors under illumination light.Quantum dot is
A kind of fluorescent nano material has color abundant, and the quantum dot colors under room lighting light are bright, send out in ultraviolet light irradiation
Fluorescence out.In CdSe quantum dot preparation process, with the progress of reaction, the CdSe quantum dot of different reaction time formation
Size is also different, also different to the absorption of visible light, can show different colors under illumination light indoors.According in reaction
The color of CdSe quantum dot solution can quickly judge the process of reaction.Therefore under the room lighting light of CdSe quantum dot solution
Colour developing result be of great significance.It usually requires to develop the color under picture and ultraviolet light irradiation under quantum dot indoors illumination light
Fluorescence picture compares.
According to the fluorescent characteristic of CdSe quantum dot, excitation wavelength selects 365 nanometers, and the excitation light source 9 is using double
365nm ultraviolet LED bank light, bank light length is 30 centimetres, with unstressed configuration background board 10,11 similar length of unstressed configuration positioning base;
Image collector 8 used is the USB camera that resolution ratio is 1920 × 1080 pixels, and lens focus 2.5mm can be clapped with video
It takes the photograph, shooting speed is 30 frame per second;Unstressed configuration background board 10 and unstressed configuration positioning base 11 used uses white polytetrafluoroethylpipe
It is process.
The sample bottle for the CdSe quantum dot sample that ten fill the formation of differential responses time is sequentially placed into unstressed configuration positioning
On 11 location hole of pedestal.Image collector 8 is connected through data transmission interface 7 with computer end, opening image collector 8 controls soft
Part opens wide chest, in situation of the computer end energy observing samples under normal indoor illumination light, and can carry out photo to it at any time
Interception.Fig. 3 is the colour developing picture under CdSe quantum dot indoors illumination light, and excitation light source 9 is closed at this time.
The fluorescence photo of the different CdSe quantum dots of embodiment 3.
Device in embodiment 2 is used to measure the case where CdSe quantum dot is in the work of 365nm ultraviolet lamp.Quantum dot is
A kind of fluorescent nano material, when having light beam irradiation to get on, electrons are transitted to higher energy level by excitation.When this
When a little electronics return to original lower energy level, the light of specific wavelength, i.e. fluorescence can be emitted.According to the fluorescence of CdSe quantum dot
Characteristic, excitation wavelength select 365 nanometers, and the excitation light source 9 uses double 365nm ultraviolet LED bank light, and bank light length is 30
Centimetre, with unstressed configuration background board 10,11 similar length of unstressed configuration positioning base;It is 1920 that image collector 8 used, which is resolution ratio,
The USB camera of × 1080 pixels, lens focus 2.5mm can be 30 frame per second with video capture, shooting speed;Used
Unstressed configuration background board 10 and unstressed configuration positioning base 11 are process with white polytetrafluoroethylpipe.
The vial for the CdSe quantum dot sample that ten fill the formation of differential responses time is sequentially placed into unstressed configuration positioning
On 11 location hole of pedestal.Image collector 8 is connected through data transmission interface 7 with computer end, opening image collector 8 controls soft
Part closes case lid 2 and presses lock 1, and Portable visual fluorescence detection device is in sealing, is protected from light state, connects 220V electricity
Supply socket 6 opens double 365nm ultraviolet LED bank light, generates under 365nm ultraviolet excitation in computer end energy observing samples
Fluorescence, and photo interception can be carried out to it at any time.Fig. 4 is the fluorogram of the CdSe quantum dot formed the differential responses time
Piece.
Fig. 3 and Fig. 4 shows with the colour developing photo and corresponding fluorescence under batch of 10 samples indoors illumination light
Photo, by comparison, it can be seen that the position of each sample remains unchanged, and unstressed configuration background board and unstressed configuration positioning base can be with
The contrast of the colour developing photo under room lighting light is increased, and it is apparent glimmering without generating under the excitation of 365nm ultraviolet LED bank light
Light background, the edge of the sample (sample 1 and sample 10) of two sides is without obvious distortion.
Comprehensive above-described embodiment can be seen that a kind of Portable visual fluorescence detection device that the present invention mentions, operation letter
Single, high reliablity, comparativity is strong between batch sample, can be well by quantum dot in the case where not changing sample position
Room lighting radiograph and fluorescence photo be compared, reject in time because the factors such as mixed and disorderly, the shooting angle transformation of background by
This bring detection error.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art within the technical scope of the present disclosure, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (5)
1. a kind of Portable visual fluorescence detection device, which is characterized in that the device includes with lower component: lock (1), case lid
(2), hinge (3), cabinet (4), handle (5), power interface (6), data transmission interface (7), image collector (8), exciting light
Source (9), unstressed configuration background board (10), unstressed configuration positioning base (11);The device passes through hinge by lid section and box portion
(3) it is formed by connecting, the outer surface of a side of lid section is equipped with lock (1), unstressed configuration background board (10) one side patch completely
It closes and is connected with the inner surface of hinge (3) side in cabinet (4), be equipped with unstressed configuration positioning base in the lower section of unstressed configuration background board (10)
(11), side is bonded with unstressed configuration background board (10), and bottom surface is fitted in cabinet (4) bottom surface, and unstressed configuration positioning base (11) exists
Several location holes, cabinet (4) side inner surface centre bit opposite with unstressed configuration background board (10) are equipped with plane perpendicular direction
It has installed image collector (8), on the side, the excitation light source (9) of strip, excitation is equipped with above image collector (8)
Light source (9) is parallel with tank floor, is equipped on the outside of the side of excitation light source (9) and is equipped with handle (5), is connected with the side another
A side is equipped with power interface (6) and data transmission interface (7).
2. a kind of Portable visual fluorescence detection device according to claim 1, which is characterized in that the exciting light
The length in source (9) is greater than or equal to unstressed configuration positioning base (11) length, and excitation light source (9) is row's formula or tubular type excitation light source.
3. a kind of Portable visual fluorescence detection device according to claim 1, which is characterized in that the exciting light
The wavelength in source (9) is 190~400nm.
4. a kind of Portable visual fluorescence detection device according to claim 1 to 3, which is characterized in that described without glimmering
The length of light background board (10) is greater than or equal to the length of unstressed configuration positioning base (11).
5. a kind of Portable visual fluorescence detection device according to claim 4, which is characterized in that the unstressed configuration
The material of background board (10) and unstressed configuration positioning base (11) is polytetrafluoroethylene (PTFE).
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Citations (4)
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CN105092543A (en) * | 2014-05-12 | 2015-11-25 | 绍兴安尼特微电子科技有限公司 | Portable fluorescence quantitative PCR detector |
CN206173319U (en) * | 2016-10-09 | 2017-05-17 | 上海交通大学医学院附属仁济医院 | Portable micro -fluidic chip LAMP visual detection appearance |
CN206684046U (en) * | 2017-04-27 | 2017-11-28 | 南京微测生物科技有限公司 | The quantitative quick analytic instrument of portable food safety fluorescent |
CN207408303U (en) * | 2017-09-04 | 2018-05-25 | 西安玻尔光电科技有限公司 | A kind of Portable fluorescence immunity analysis instrument |
-
2019
- 2019-03-18 CN CN201910201692.9A patent/CN109813694A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105092543A (en) * | 2014-05-12 | 2015-11-25 | 绍兴安尼特微电子科技有限公司 | Portable fluorescence quantitative PCR detector |
CN206173319U (en) * | 2016-10-09 | 2017-05-17 | 上海交通大学医学院附属仁济医院 | Portable micro -fluidic chip LAMP visual detection appearance |
CN206684046U (en) * | 2017-04-27 | 2017-11-28 | 南京微测生物科技有限公司 | The quantitative quick analytic instrument of portable food safety fluorescent |
CN207408303U (en) * | 2017-09-04 | 2018-05-25 | 西安玻尔光电科技有限公司 | A kind of Portable fluorescence immunity analysis instrument |
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Application publication date: 20190528 |