CN109797188A - A kind of cell detection method - Google Patents
A kind of cell detection method Download PDFInfo
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- CN109797188A CN109797188A CN201910027890.8A CN201910027890A CN109797188A CN 109797188 A CN109797188 A CN 109797188A CN 201910027890 A CN201910027890 A CN 201910027890A CN 109797188 A CN109797188 A CN 109797188A
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Abstract
The invention discloses a kind of cell detection methods, comprising: extracts sample；Make L1 sample and DNA ploidy body and content analysis sample respectively of sample；Detect expression status of the L1 in host cell in L1 sample, detect the DNA ploidy body and content in DNA ploidy body and content analysis sample, according to the performance of cell of the L1 in the expression status and DNA ploidy body and content judgement sample in host cell, the performance of cell includes normal condition, suspicious state and abnormality.Cell detection method provided by the invention judges whether cell is abnormal according to L1 expression status and DNA ploidy body and content, so as to accurately detect the abnormality of cell.
Description
Technical field
The present invention relates to cell detection field more particularly to a kind of cell detection methods.
Background technique
Current cell detection method judges whether cell is abnormal generally by the DNA ploidy body of detection sample, due to
It is influenced by other uncertain factors more, causes judging result not accurate enough.Also have at present and is expressed by single inspection L1 to sentence
Disconnected cell whether Yi Chang judgment method, can not judge when detecting that L1 is expressed as feminine gender cell to be abnormal or normal, from
And it is difficult to judge meaning when L1 is expressed as feminine gender, when L1 expression status is negative, judging result is also inaccurate.
Summary of the invention
For overcome the deficiencies in the prior art, the purpose of the present invention is to provide a kind of cell detection methods, existing to solve
There is the problem of cell detection method testing result inaccuracy.
The purpose of the present invention adopts the following technical scheme that realization:
A kind of cell detection method, comprising:
Extract sample；
L1 sample and DNA ploidy body and content analysis sample are made respectively with the sample；
Expression status of the L1 in host cell in the L1 sample is detected, the DNA ploidy body and content analysis sample are detected
DNA ploidy body and content in this judge institute according to expression status of the L1 in host cell and the DNA ploidy body and content
The performance of the cell in sample is stated, the performance of the cell includes normal condition, suspicious state and abnormality.
Further, expression status of the L1 in host cell in the L1 sample is detected, the DNA ploidy body is detected and contains
DNA ploidy body and content in amount analysis sample, according to expression status of the L1 in host cell and the DNA ploidy body with contain
Amount judges that the performance of the cell in the sample includes:
Detect expression status of the L1 in host cell in the L1 sample；
If expression status of the L1 in host cell is positive, cell is normal condition, stops detection；
If expression status of the L1 in host cell is negative, detect in the DNA ploidy body and content analysis sample
DNA ploidy body and content judge the performance of cell according to DNA ploidy body and content.
Further, if expression status of the L1 in host cell is negative, the DNA ploidy body and content analysis are detected
DNA ploidy body and content in sample, the performance for judging cell according to DNA ploidy body and content include:
If expression status of the L1 in host cell is negative, DNA ploidy body > 4.5, DNA content > 7.18pg, then cell is
Abnormality, chromosome deficiency；
If expression status of the L1 in host cell is negative, 2.2<DNA ploidy body≤4.5, DNA content>7.18pg, then carefully
Born of the same parents are suspicious state, and chromosomes of cell nuclei missing is unobvious；
If expression status of the L1 in host cell is negative, DNA ploidy body≤2.2, then cell is DNA content≤7.18pg
Normal condition.
Further, detecting expression status of the L1 in host cell in the L1 sample includes:
Multiple visuals field of flying-spot microscope, if the L1 antigen presentation of at least one cell, the L1 of the L1 sample is in place
Expression status in chief cell is the positive；If the cell in multiple visuals field is all without L1 antigen presentation, the L1 of the L1 sample exists
Expression status in host cell is feminine gender.
Further, detect the DNA ploidy body with the DNA ploidy body in content analysis sample includes: with content
The DNA ploidy body of each cell is marked with content, and is united to the DNA ploidy body of all cells with content
Meter, calculates the DNA ploidy body and content of each cell, marks DNA ploidy body and content is significantly greater than the cell of threshold value.
Further, making L1 sample and DNA ploidy body respectively with content analysis sample with the sample includes:
Two subsamples are extracted from the same portion sample；
Two subsamples are dyed respectively, form L1 sample and DNA ploidy body and content analysis sample.
Compared with prior art, the beneficial effects of the present invention are: pass through expression of the L1 in host cell of detection sample
State and DNA ploidy body and content judge whether cell is abnormal according to L1 expression status and DNA ploidy body and content, so as to standard
The really performance of detection cell.
Detailed description of the invention
Fig. 1 is cell detection method flow chart provided in an embodiment of the present invention；
Fig. 2 is the flow chart of the performance of judgement cell provided in an embodiment of the present invention.
Specific embodiment
In the following, being described further in conjunction with attached drawing and specific embodiment to the present invention, it should be noted that not
Under the premise of conflicting, new implementation can be formed between various embodiments described below or between each technical characteristic in any combination
Example.
As shown in Figure 1, cell detection method provided in an embodiment of the present invention, comprising:
Step S101: sample is extracted.
Preferably, sample can be epithelium of cervix uteri cast-off cells.
Step S102: L1 sample and DNA ploidy body and content analysis sample are made respectively with the sample.
Preferably, L1 sample and DNA ploidy body and content analysis sample are to extract two subsamples from same a sample,
After extracting subsample, two subsamples are dyed respectively, forms L1 sample and DNA ploidy body and content analysis sample.Its
In, L1 sample is dyed using AEC or fluorescent staining method, DNA ploidy body and content analysis sample are dyed using DNA-Feulgen
Method.
Step S103: expression status of the L1 in host cell in the L1 sample is detected, the DNA ploidy body is detected and contains
DNA ploidy body and content in amount analysis sample, according to expression status of the L1 in host cell and the DNA ploidy body with contain
Amount judges the performance of the cell in the sample, and the performance of the cell includes normal condition, suspicious state and abnormality.Knot
It closes L1 expression status and DNA ploidy body and whether content comprehensive descision cell is abnormal, accuracy rate is higher.
Preferably, the step includes:
Step S201: expression status of the L1 in host cell in the L1 sample is detected.
Preferably, the step includes: multiple visuals field of flying-spot microscope, if the L1 antigen presentation of at least one cell,
Expression status of the L1 of the L1 sample in host cell is the positive；If cell in multiple visuals field all without L1 antigen presentation,
Then expression status of the L1 of the L1 sample in host cell is feminine gender.
Step S202: if expression status of the L1 in host cell is positive, cell is normal, stops detection.
Step S203: if expression status of the L1 in host cell is negative, the DNA ploidy body and content analysis are detected
DNA ploidy body and content in sample judge the performance of cell according to DNA ploidy body and content.
Preferably, for DNA ploidy body and content analysis sample, scanned samples cell judges cell DNA by cellular morphology
Times body and content, are marked DNA ploidy body and the content of each cell, and carry out to the DNA ploidy body of all cells and content
Statistics, calculates the DNA ploidy body and content of each cell, marks DNA ploidy body and content is significantly greater than the cell of threshold value, according to system
The abnormal level peak meter of meter calculates the DNA ploidy body and content of sample.
Step S204: if expression status of the L1 in host cell is negative, DNA ploidy body > 4.5, DNA content > 7.18pg,
Then cell is abnormal, chromosome deficiency.Specially L1 protein expression disappears, and DNA ploidy body and content are abnormal.
Step S205: if expression status of the L1 in host cell is negative, 2.2<DNA ploidy body≤4.5, DNA content>
7.18pg, then cell is suspicious state, and chromosomes of cell nuclei missing is unobvious.Specially L1 albumen is free or is integrated in nucleus
Outside, DNA ploidy body is more abnormal, according to the different abnormality of sample, can implement different interference methods to cell.
Step S206: if expression status of the L1 in host cell is negative, DNA ploidy body≤2.2, DNA content≤
7.18pg, then cell is normal.
Cell detection method provided by the invention, by detecting expression status and DNA of the L1 of sample in host cell
Whether extremely times body and content judges cell according to L1 expression status and DNA ploidy body and content, so as to accurately detect cell
Performance.
The above embodiment is only the preferred embodiment of the present invention, and the scope of protection of the present invention is not limited thereto,
The variation and replacement for any unsubstantiality that those skilled in the art is done on the basis of the present invention belong to institute of the present invention
Claimed range.
Claims (6)
1. a kind of cell detection method characterized by comprising
Extract sample；
L1 sample and DNA ploidy body and content analysis sample are made respectively with the sample；
Expression status of the L1 in host cell in the L1 sample is detected, is detected in the DNA ploidy body and content analysis sample
DNA ploidy body and content, the sample is judged according to expression status of the L1 in host cell and the DNA ploidy body and content
The performance of cell in this, the performance of the cell includes normal condition, suspicious state and abnormality.
2. cell detection method according to claim 1, which is characterized in that detect in the L1 sample L1 in host cell
In expression status, the DNA ploidy body and content in the DNA ploidy body and content analysis sample are detected, according to the L1 in host
Expression status and the DNA ploidy body in cell and content judge that the performance of the cell in the sample includes:
Detect expression status of the L1 in host cell in the L1 sample；
If expression status of the L1 in host cell is positive, cell is normal condition, stops detection；
If expression status of the L1 in host cell is negative, the DNA ploidy in the DNA ploidy body and content analysis sample is detected
Body and content judge the performance of cell according to DNA ploidy body and content.
3. cell detection method according to claim 2, which is characterized in that if expression status of the L1 in host cell is in
Feminine gender then detects DNA ploidy body and content in the DNA ploidy body and content analysis sample, is judged according to DNA ploidy body and content thin
The performance of born of the same parents includes:
If expression status of the L1 in host cell is negative, DNA ploidy body > 4.5, DNA content > 7.18pg, then cell is abnormal
State, chromosome deficiency；
If expression status of the L1 in host cell is negative, 2.2<DNA ploidy body≤4.5, DNA content>7.18pg, then cell is
Suspicious state, chromosomes of cell nuclei missing are unobvious；
If expression status of the L1 in host cell is negative, DNA ploidy body≤2.2, DNA content≤7.18pg, then cell is positive
Normal state.
4. cell detection method according to claim 2, which is characterized in that detect in the L1 sample L1 in host cell
In expression status include:
Multiple visuals field of flying-spot microscope, if the L1 antigen presentation of at least one cell, the L1 of the L1 sample is thin in host
Expression status in born of the same parents is the positive；If the cell in multiple visuals field is all without L1 antigen presentation, the L1 of the L1 sample is in host
Expression status in cell is feminine gender.
5. cell detection method according to claim 1, which is characterized in that detect the DNA ploidy body and content analysis sample
DNA ploidy body in this includes: with content
The DNA ploidy body of each cell is marked with content, and the DNA ploidy body of all cells is counted with content, is counted
The DNA ploidy body and content for calculating each cell, mark DNA ploidy body and content is significantly greater than the cell of threshold value.
6. cell detection method according to claim 1, which is characterized in that with the sample make respectively L1 sample and
DNA ploidy body includes: with content analysis sample
Two subsamples are extracted from the same portion sample；
Two subsamples are dyed respectively, form L1 sample and DNA ploidy body and content analysis sample.
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| CN201910027890.8A CN109797188A (en) | 2019-01-11 | 2019-01-11 | A kind of cell detection method |
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|---|---|---|---|---|
| US20110091867A1 (en) * | 2008-07-03 | 2011-04-21 | Staten Island University | Method to identify and predict disease progression of human papilloma virus-infected lesions |
| CN107827960A (en) * | 2017-10-26 | 2018-03-23 | 中国食品药品检定研究院 | The quantitative detecting method of HPV L1 albumen |
| CN108507850A (en) * | 2018-04-10 | 2018-09-07 | 南京福怡科技发展股份有限公司 | A kind of cytology DNA ploidy body staining kit and application |
-
2019
- 2019-01-11 CN CN201910027890.8A patent/CN109797188A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110091867A1 (en) * | 2008-07-03 | 2011-04-21 | Staten Island University | Method to identify and predict disease progression of human papilloma virus-infected lesions |
| CN107827960A (en) * | 2017-10-26 | 2018-03-23 | 中国食品药品检定研究院 | The quantitative detecting method of HPV L1 albumen |
| CN108507850A (en) * | 2018-04-10 | 2018-09-07 | 南京福怡科技发展股份有限公司 | A kind of cytology DNA ploidy body staining kit and application |
Non-Patent Citations (3)
| Title |
|---|
| "DNA倍体分析和HPV检测在宫颈ASCUS诊断中的意义" * |
| 张佳立等: "液基细胞学涂片DNA倍体分析与HPV检测在宫颈病变筛查中的比较" * |
| 马丽丽等: "TCT、DNA倍体定量分析及HPV检测筛查宫颈病变价值研究" * |
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