CN109781647A - A kind of differentiating method of caulis piperis futokadsurae He Hance Pepper Stem and Leaf - Google Patents
A kind of differentiating method of caulis piperis futokadsurae He Hance Pepper Stem and Leaf Download PDFInfo
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- CN109781647A CN109781647A CN201811526081.3A CN201811526081A CN109781647A CN 109781647 A CN109781647 A CN 109781647A CN 201811526081 A CN201811526081 A CN 201811526081A CN 109781647 A CN109781647 A CN 109781647A
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- leaf
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- caulis piperis
- piperis futokadsurae
- pepper stem
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Abstract
The invention discloses the differentiating methods of a kind of caulis piperis futokadsurae He Hance Pepper Stem and Leaf, comprising: sample strip preparation step: by sample comminution, sieving, grind into powder；Potassium bromide is added by several times in the powder, while in a heated condition that powder and potassium bromide ground and mixed is uniform, obtains mixed-powder；Mixed-powder is tabletted, obtain sample strip；IR spectrum scanning step: sample strip is subjected to IR spectrum scanning, obtains infrared spectrogram；Distinguish step: observation infrared spectrogram, in 1047cm‑1There is absorption and in 1077cm‑1What is do not absorbed is then caulis piperis futokadsurae；In 1077cm‑1And 1031cm‑1There is absorption then Wei Hance Pepper Stem and Leaf；This method has quickly, the strong feature of simplicity, strong operability, characteristic by fourier transform infrared spectroscopy, can variation from the point of view of macroscopic view to all chemical components of Chinese medicine, the distinctive points both found out realize the quick differentiation of the two.
Description
Technical field
The present invention relates to the differentiating methods of a kind of caulis piperis futokadsurae He Hance Pepper Stem and Leaf, belong to plant classification technical field.
Background technique
Caulis piperis futokadsurae derives from the drying rattan of Piperaceae plant wind rattan Piper kadsura (Choisy) Ohwi.Vegetal inspired
State is " woody climber；Stem has vertical rib, by thin hair when young, takes root on section.Leaf nearly keratin, the white gland point of tool, oval or long avette,
6-12 centimetres long, 3.5-7 centimetres wide, the short point or blunt in top, base portion is heart-shaped, and dilute blunt circle, the outside of belly is hairless, and the back side is usually by short soft
Hair；Vein 5, base goes out or nearly base portion issues, and outermost 1 pair is thin and delicate, not very significantly, the scun camber arch that middle arteries middle and upper part issues；Leaf
Handle is 1-1.5 centimetres long, sometimes coat；Leaf sheath, which is only limitted to base portion, to be had.Flower unisexuality, dioecism are gathered into leaf to raw fringe
Locusta.Male inflorescence is 3-5.5 centimetres long；Bract is round, and nearly stockless, peltate, about 1 millimeter of diameter, edge is irregular, and the outside of belly is white
Color coarse wool；2-3 pieces of stamen, filigree is short.Female inflorescence is shorter than blade；Total bennet and petiole are isometric；Bract and rachis and male inflorescence
It is identical；Ovary is spherical, and from life, column cap 3-4 is linear, by pubescence.Berry is spherical, isabelline, and 3-4 millimeters of diameter.Florescence 5-
August ".
Hance Pepper Stem and Leaf is often taken as caulis piperis futokadsurae to use in south, from Piperaceae plant Hance Pepper Stem and Leaf Piper hancei Maxi m.'s
Dry branches and leaves.The two form is closely similar, it becomes difficult to distinguish.Even micro- powder identifies, it has been found that the microscopic features one of the two
It causes, is not different.
Summary of the invention
For overcome the deficiencies in the prior art, the first purpose of this invention is to provide a kind of caulis piperis futokadsurae He the area of Hance Pepper Stem and Leaf
Divide method, this method has quickly, the strong spy of simplicity, strong operability, characteristic by fourier transform infrared spectroscopy
Point, can variation from the point of view of macroscopic view to all chemical components of Chinese medicine, the distinctive points both found out, that both realizes is quick
It distinguishes.
Achieving the object of the present invention can be reached by adopting the following technical scheme that: a kind of differentiation side of caulis piperis futokadsurae He Hance Pepper Stem and Leaf
Method, comprising:
Sample strip preparation step: by sample comminution, sieving, grind into powder；Potassium bromide is added by several times in the powder, simultaneously
It is in a heated condition that powder and potassium bromide ground and mixed is uniform, obtain mixed-powder；Mixed-powder is tabletted, obtain sample
Product piece；
IR spectrum scanning step: sample strip is subjected to IR spectrum scanning, obtains infrared spectrogram；
Distinguish step: observation infrared spectrogram, in 1047cm-1There is absorption and in 1077cm-1What is do not absorbed is then sea
Wind rattan；In 1077cm-1And 1031cm-1There is absorption then Wei Hance Pepper Stem and Leaf.
Further, it in sample strip preparation step, will be sieved with 100 mesh sieve after sample comminution.
Further, in sample strip preparation step, sample is ground with mortar.
Further, mortar is agate mortar.
Further, it in sample strip preparation step, is heated with infrared baking lamp.
Further, with tablet press machine that mixed-powder is tabletted in sample strip preparation step.
Further, under conditions of pressure 8t, time 30s that mixed-powder is tabletted.
Further, in IR spectrum scanning step, IR spectrum scanning range is 4000cm-1-400cm-1, scanned
Carbon dioxide and vapor interference are deducted in journey in real time.
Further, in IR spectrum scanning step, 16 times IR spectrum scanning is cumulative.
Further, in IR spectrum scanning step, resolution ratio 4cm-1。
Compared with prior art, the beneficial effects of the present invention are:
1, differentiating method of the invention has quickly by fourier transform infrared spectroscopy, simplicity, strong operability,
The strong feature of characteristic, can variation from the point of view of macroscopic view to all chemical components of Chinese medicine, the distinctive points both found out are real
Both existing quick differentiation；
2, in differentiating method of the invention, ground and mixed is performed under heating conditions with sample powder and potassium bromide, than passing
The hybrid mode of system is more efficient, and mixing is more evenly.
Detailed description of the invention
Fig. 1 is the one-dimensional infrared spectrogram of embodiment 1；
Fig. 2 is 2 second derivative spectra figure of embodiment.
Specific embodiment
In the following, being described further in conjunction with attached drawing and specific embodiment to the present invention:
A kind of differentiating method of caulis piperis futokadsurae He Hance Pepper Stem and Leaf, comprising:
Sample strip preparation step: sample comminution sieves with 100 mesh sieve, with agate mortar grind into powder；In the powder by several times
Potassium bromide is added, while powder and potassium bromide ground and mixed is uniform under conditions of being heated with infrared baking lamp, is mixed
Powder；It is under conditions of pressure 8t, time 30s that mixed-powder is tabletted with tablet press machine, obtain sample strip；
IR spectrum scanning step: sample strip is subjected to IR spectrum scanning, IR spectrum scanning range is 4000cm-1-
400cm-1, carbon dioxide and vapor interference are deducted in scanning process in real time, 16 times IR spectrum scanning is cumulative, and resolution ratio is
4cm-1, obtain infrared spectrogram；
Distinguish step: observation infrared spectrogram, in 1047cm-1There is absorption and in 1077cm-1What is do not absorbed is then sea
Wind rattan；In 1077cm-1And 1031cm-1There is absorption then Wei Hance Pepper Stem and Leaf.
Embodiment 1:
One-dimensional infrared spectroscopy is standardized with blank:
Blank preparation: potassium bromide is crushed, under conditions of being heated with infrared baking lamp, bromine is ground into agate mortar
Change potassium powder；It is under conditions of pressure 8t, time 30s that potassium bromide powder is tabletted with tablet press machine, obtain blank；
The preparation of caulis piperis futokadsurae standard film: caulis piperis futokadsurae standard items being crushed, are sieved with 100 mesh sieve, with agate mortar grind into powder；
Potassium bromide is added by several times in the powder, while powder and potassium bromide ground and mixed is equal under conditions of being heated with infrared baking lamp
It is even, obtain mixed-powder；It is under conditions of pressure 8t, time 30s that mixed-powder is tabletted with tablet press machine, obtain caulis piperis futokadsurae
Standard film；
The preparation for preparing: Hance Pepper Stem and Leaf standard film of Hance Pepper Stem and Leaf standard film is identical as preparing for caulis piperis futokadsurae standard film；
IR spectrum scanning step: carrying out IR spectrum scanning for blank, caulis piperis futokadsurae standard film He Hance Pepper Stem and Leaf standard film, red
External spectrum scanning range is 4000cm-1-400cm-1, carbon dioxide and vapor interference are deducted in scanning process in real time, 16 times red
External spectrum scanning is cumulative, resolution ratio 4cm-1, one-dimensional infrared spectrogram is obtained, blank spectrogram is used for for excluding in sample
The absorption of potassium bromide；
Spectrogram processing: obtained one-dimensional infrared spectrogram is subjected to coordinate transformation, ordinate is absorbance, baseline school
Just, the intensity threshold of spectral normalization, identification infrared light spectral peak is set as 0.01A.
Embodiment 2:
The identification of map:
Obtained map as shown in Figure 1, compare in figure it is known that caulis piperis futokadsurae He Hance Pepper Stem and Leaf in 1155cm-1-1031cm-1's
Absorption peak and peak shape are different, and " trapezoidal " absorption of , Hance Pepper Stem and Leaf is better than caulis piperis futokadsurae as a whole.Caulis piperis futokadsurae is in 1077cm-1No
Absorbing , Hance Pepper Stem and Leaf has absorption；Caulis piperis futokadsurae is in 1047cm-1Have and absorbs , Hance Pepper Stem and Leaf in 1031cm-1There is absorption；In 1155cm-1Locate , Hance Pepper Stem and Leaf
Absorption is better than caulis piperis futokadsurae.
The calculating of second derivative spectra: second dervative is calculated to processed one-dimensional infrared spectroscopy, smoothly to get two at 13 points
Order derivative spectrum.The threshold value at instrument automatic identification peak is set as 0.0005A (peak intensity).As shown in Fig. 2, in second dervative, sea wind
Rattan is in 1485cm-1There is absorption, Hance Pepper Stem and Leaf does not have Er；Hance Pepper Stem and Leaf is in 1493cm-1There is absorption, caulis piperis futokadsurae does not have.The two is in 1120cm-1-
1000cm-1Absorption it is inconsistent, whole peak shape is all different, caulis piperis futokadsurae mainly in 1097cm-1There is absorption , Hance Pepper Stem and Leaf mainly to exist
1069cm-1There is absorption.Illustrate that the two differs greatly, therefore 1120cm can be chosen-1-1000cm-1As the feature for identifying the two
Map.
For those skilled in the art, it can make other each according to the above description of the technical scheme and ideas
Kind is corresponding to be changed and deforms, and all these change and deform the protection model that all should belong to the claims in the present invention
Within enclosing.
Claims (10)
1. the differentiating method of a kind of caulis piperis futokadsurae He Hance Pepper Stem and Leaf, characterized by comprising:
Sample strip preparation step: by sample comminution, sieving, grind into powder；Potassium bromide is added by several times in the powder, while adding
It is under heat condition that powder and potassium bromide ground and mixed is uniform, obtain mixed-powder；Mixed-powder is tabletted, obtain sample
Piece；
IR spectrum scanning step: sample strip is subjected to IR spectrum scanning, obtains infrared spectrogram；
Distinguish step: observation infrared spectrogram, in 1047cm-1There is absorption and in 1077cm-1What is do not absorbed is then caulis piperis futokadsurae；
In 1077cm-1And 1031cm-1There is absorption then Wei Hance Pepper Stem and Leaf.
2. the differentiating method of caulis piperis futokadsurae as described in claim 1 He Hance Pepper Stem and Leaf, which is characterized in that, will in sample strip preparation step
It is sieved with 100 mesh sieve after sample comminution.
3. the differentiating method of caulis piperis futokadsurae as described in claim 1 He Hance Pepper Stem and Leaf, which is characterized in that in sample strip preparation step, with
Mortar grinds sample.
4. the differentiating method of caulis piperis futokadsurae as claimed in claim 3 He Hance Pepper Stem and Leaf, which is characterized in that the mortar is agate mortar.
5. the differentiating method of caulis piperis futokadsurae as described in claim 1 He Hance Pepper Stem and Leaf, which is characterized in that in sample strip preparation step, with
Infrared baking lamp is heated.
6. the differentiating method of caulis piperis futokadsurae as described in claim 1 He Hance Pepper Stem and Leaf, which is characterized in that in sample strip preparation step, use
Tablet press machine is tabletted by mixed-powder.
7. the differentiating method of caulis piperis futokadsurae as described in claim 1 He Hance Pepper Stem and Leaf, which is characterized in that in the item of pressure 8t, time 30s
It is under part that mixed-powder is tabletted.
8. the differentiating method of caulis piperis futokadsurae as described in claim 1 He Hance Pepper Stem and Leaf, which is characterized in that in IR spectrum scanning step,
IR spectrum scanning range is 4000cm-1-400cm-1, carbon dioxide and vapor interference are deducted in scanning process in real time.
9. the differentiating method of caulis piperis futokadsurae as described in claim 1 He Hance Pepper Stem and Leaf, which is characterized in that in IR spectrum scanning step,
16 times IR spectrum scanning is cumulative.
10. the differentiating method of caulis piperis futokadsurae as described in claim 1 He Hance Pepper Stem and Leaf, which is characterized in that in IR spectrum scanning step,
Resolution ratio is 4cm-1。
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103674887A (en) * | 2013-12-16 | 2014-03-26 | 张雪峰 | Analysis identification method of ophiocordyceps sinensis through infrared spectrum |
| JP5707213B2 (en) * | 2011-04-20 | 2015-04-22 | ブルカー・オプティクス株式会社 | Infrared transmission spectrum measuring device |
| CN105954222A (en) * | 2016-07-18 | 2016-09-21 | 成都中医药大学 | Identification method for Brag-zhun and faecal pellet substitutes |
| CN106323903A (en) * | 2015-06-26 | 2017-01-11 | 云南天士力帝泊洱生物茶集团有限公司 | Establishment and detection methods of infrared-spectroscopy fingerprint map of Pu'er tea extract |
| CN107091815A (en) * | 2017-05-05 | 2017-08-25 | 张方达 | A kind of method for identifying rosewood |
-
2018
- 2018-12-13 CN CN201811526081.3A patent/CN109781647A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5707213B2 (en) * | 2011-04-20 | 2015-04-22 | ブルカー・オプティクス株式会社 | Infrared transmission spectrum measuring device |
| CN103674887A (en) * | 2013-12-16 | 2014-03-26 | 张雪峰 | Analysis identification method of ophiocordyceps sinensis through infrared spectrum |
| CN106323903A (en) * | 2015-06-26 | 2017-01-11 | 云南天士力帝泊洱生物茶集团有限公司 | Establishment and detection methods of infrared-spectroscopy fingerprint map of Pu'er tea extract |
| CN105954222A (en) * | 2016-07-18 | 2016-09-21 | 成都中医药大学 | Identification method for Brag-zhun and faecal pellet substitutes |
| CN107091815A (en) * | 2017-05-05 | 2017-08-25 | 张方达 | A kind of method for identifying rosewood |
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Application publication date: 20190521 |