CN109777769A - The source tooth screening technique extracted for dental pulp stem cell - Google Patents
The source tooth screening technique extracted for dental pulp stem cell Download PDFInfo
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- CN109777769A CN109777769A CN201711120603.5A CN201711120603A CN109777769A CN 109777769 A CN109777769 A CN 109777769A CN 201711120603 A CN201711120603 A CN 201711120603A CN 109777769 A CN109777769 A CN 109777769A
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Abstract
The invention discloses the source tooth screening techniques extracted for dental pulp stem cell.The study result show that: the deciduous teeth to fall off naturally are the tooth without dental pulp, no stem cell；Deciduous teeth within the loosening of II degree, 2/3 or more External apical root resorption or root of the tooth fully absorb but pulp chamber bottom it is complete in the presence of, dental pulp stem cell success rate of extracting is high, and on the eruption of permanent teeth without too big influence, acquires opportunity for optimal deciduous teeth source tooth.Method of the invention is to provide fundamental basis on the acquisition opportunity of clinical deciduous teeth dental pulp stem cell source tooth from now on.
Description
Technical field
The invention belongs to field of biotechnology, and in particular to the source tooth screening technique extracted for dental pulp stem cell.
Background technique
Source for mesenchymal stem cells is in Various Tissues, such as marrow, dental pulp, placenta, umbilical cord and fat, wherein coming from deciduous teeth
The mescenchymal stem cell of dental pulp has powerful stemness and biological activity, and no rejection, immunoregulation capability is strong, is very
One of outstanding biological therapy cell origin.Since the mescenchymal stem cell from deciduous teeth dental pulp is drawn materials conveniently, it is suitable for advising greatly
Mould culture, therefore good basis has been established for the industrialization of dental pulp stem cell.
Due to the continuous improvement of China's national product, life style constantly improves, kids' recipe compared with the past of today
Reduce many harder adult recipe ingredients, therefore influence of the mastication processes to deciduous teeth greatly weakens, leads to nowadays children's
Deciduous teeth are not easy the replacement that normally falls off in the past relatively, and are prone to be detained, therefore generally require to hospital to be pulled out.In mistake
It goes, the deciduous teeth pulled out largely are abandoned as Biohazard Waste, and the discovery of dental pulp stem cell and progress, so that these are useless
Gurry as dental pulp stem cell important sources and obtain recycling chance, for dental pulp stem cell industrialization provide resource protect
Card.
Summary of the invention
The success that the technical problem to be solved by the present invention is to how select suitable source tooth to guarantee dental pulp stem cell extraction
Rate obtains enough and good dental pulp stem cells.
In order to solve the above technical problems, present invention firstly provides source teeth as the raw material for extracting or preparing dental pulp stem cell
In application:
The source tooth meet it is following 1) and 2) described in condition:
1) within II degree loosens；
2) it is gone bad without dental caries or dental caries bad degree is below middle dental caries.
The present invention also provides the source teeth for meeting above-mentioned condition to extract or prepare the application in dental pulp stem cell.
In above-mentioned application, the II degree loosens within loosening including I degree and II degree loosens.I degree of loosening refers to tooth cheek tongue
The dynamic degree in direction (inward-outward direction) is within 1mm, the not dynamic degree in other directions；There are two types of situations for the loosening of II degree: one is tooth cheek
For the dynamic degree in tongue direction (inward-outward direction) between 1-2mm, two be that there are cheek tongue direction (inward-outward direction) and near and far directions for tooth
The dynamic degree of (left and right directions) both direction.
In above-mentioned application, the source tooth is deciduous teeth.At least one of the source tooth concretely following a1)-a5):
A1) the source tooth of the loosening of I degree and External apical root resorption 1/3；
A2) the source tooth of the loosening of I degree and External apical root resorption 1/2；
A3) the source tooth of the loosening of II degree and External apical root resorption 1/2；
A4) the source tooth of the loosening of II degree and External apical root resorption 2/3；
A5) II degree loosens and root of the tooth fully absorbs but the complete existing source tooth of pulp chamber bottom.
In practical applications, select I degree loosen to II degree loosen tooth as source tooth, judge by visual observation source tooth whether there is or not
Dental caries are gone bad and dental caries bad degree, when source tooth is gone bad without dental caries or dental caries bad degree is below middle dental caries, are acquired to source tooth, and stand after acquisition
It is put into full of in the container for saving liquid, then sealing container, is put under low temperature environment and is saved or transported.
Wherein it is preferred to which the source tooth is that II degree loosens and the deciduous teeth or II degree of External apical root resorption 2/3 loosen and root of the tooth is complete
It absorbs but the complete existing deciduous teeth of pulp chamber bottom.
Wherein it is preferred to further include the steps that carrying out disinfection to source tooth appearance with disinfectant before acquiring source tooth.Institute
The method for stating disinfection, which is concretely contained with the thimerosals such as the Iodophor wiping gargles such as corona or compound Chlorhexidine, gargles.
Wherein it is preferred to be saved or transported in the case where tooth acquisition in source is placed on (2-4) DEG C environment.
Wherein it is preferred to which source tooth is put into full of in the container for saving liquid in 10 seconds after source tooth acquires.
Wherein it is preferred to the container is the source tooth collection tube purchased from Beijing Tai Sheng Biotechnology Co., Ltd, it is described
Saving liquid is that the source tooth acquisition purchased from Beijing Tai Sheng Biotechnology Co., Ltd saves liquid.
The source tooth can also be permanent teeth or wisdom tooth, and when source tooth is permanent teeth or wisdom tooth, source tooth does not include judging that it is loosened
The step of spending, other steps and source tooth are identical when being deciduous teeth.
Before acquiring source tooth, further include the steps that screening source tooth, carrying out screening to source tooth is to guarantee that dental pulp is dry thin
Born of the same parents extract successfully crucial, and Ying Jinliang avoids select that dental caries bad (containing secondary caries), there are the teeth of obturator as source tooth；If there is
Dental caries are bad or there are obturations, it is proposed that shooting Dental X-ray film judges former dental caries bad depth.If former dental caries are badly shallow dental caries or middle dental caries, it is living to carry out dental pulp
Power test is active, can attempt to carry out stem cell storage after removing dental caries bad tissue；If former dental caries are badly deep dental caries, it is not recommended that storage,
Infection risk is big, and success rate of extracting is low.
In order to solve the above technical problems, the present invention also provides the screening techniques of the source tooth extracted for dental pulp stem cell.
Screening technique provided by the present invention for the source tooth that dental pulp stem cell extracts include choose meet it is following 1) and 2)
The source tooth of the condition:
The source tooth meet it is following 1) and 2) described in condition:
1) within II degree loosens；
2) it is gone bad without dental caries or dental caries bad degree is below middle dental caries.
In the above method, the source tooth is deciduous teeth.At least one of the source tooth concretely following a1)-a5):
A1) the source tooth of the loosening of I degree and External apical root resorption 1/3；
A2) the source tooth of the loosening of I degree and External apical root resorption 1/2；
A3) the source tooth of the loosening of II degree and External apical root resorption 1/2；
A4) the source tooth of the loosening of II degree and External apical root resorption 2/3；
A5) II degree loosens and root of the tooth fully absorbs but the complete existing source tooth of pulp chamber bottom.
Actually screening in, select I degree loosen to II degree loosening tooth as source tooth, judge by visual observation source tooth whether there is or not
Dental caries are gone bad and dental caries bad degree, when source tooth is gone bad without dental caries or dental caries bad degree is below middle dental caries, are acquired to source tooth, and stand after acquisition
It is put into full of in the container for saving liquid, then sealing container, is put under low temperature environment and is saved or transported.
Wherein it is preferred to which the source tooth is that II degree loosens and the deciduous teeth or II degree of External apical root resorption 2/3 loosen and root of the tooth is complete
It absorbs but the complete existing deciduous teeth of pulp chamber bottom.
Wherein it is preferred to further include the steps that carrying out disinfection to source tooth appearance with disinfectant before acquiring source tooth.Institute
The method for stating disinfection, which is concretely contained with the thimerosals such as the Iodophor wiping gargles such as corona or compound Chlorhexidine, gargles.
Wherein it is preferred to be saved or transported in the case where tooth acquisition in source is placed on (2-4) DEG C environment.
Wherein it is preferred to which source tooth is put into full of in the container for saving liquid in 10 seconds after source tooth acquires.
Wherein it is preferred to the container is the source tooth collection tube purchased from Beijing Tai Sheng Biotechnology Co., Ltd, it is described
Saving liquid is that the source tooth acquisition purchased from Beijing Tai Sheng Biotechnology Co., Ltd saves liquid.
The source tooth can also be permanent teeth or wisdom tooth, not include judgement in screening technique when source tooth is permanent teeth or wisdom tooth
The step of its Movable degree, other steps and source tooth are identical when being deciduous teeth.
The screening technique further includes avoiding selecting that dental caries bad (containing secondary caries), there are the teeth of obturator as source tooth as far as possible
The step of.If there is dental caries are bad or there are obturations, it is proposed that shooting Dental X-ray film judges former dental caries bad depth.If former dental caries be badly shallow dental caries or in
Dental caries, progress pulp vitality test is active, can attempt to carry out stem cell storage after removing dental caries bad tissue；If former dental caries are badly deep dental caries,
Then it is not recommended that storage, infection risk is big, and success rate of extracting is low.
The above method extract or prepare in dental pulp stem cell application also belong to protection scope of the present invention.
The case where extracting to source tooth dental pulp stem cell statistical analysis discovery: 32, the source tooth of External apical root resorption 1/3, dental pulp are dry thin
Born of the same parents extract successfully 30, success rate 93.75%；35, the source tooth of External apical root resorption 1/2, dental pulp stem cell extract successfully 32, at
Power 91.42%；59, the source tooth of External apical root resorption 2/3, dental pulp stem cell extract successfully 54, success rate 91.53%；Root of the tooth is complete
Hypersorption but 37, the complete source tooth of pulp chamber bottom, dental pulp stem cell extracts successfully 34, success rate 91.89%；Naturally it falls off
Deciduous teeth 10, dental pulp stem cell extracts successfully 0.Except the deciduous teeth acquisition success rate to fall off naturally is low, other acquisition success rates are poor
It is different without obvious statistical significance.To statistical analysis discovery the case where tooth dystopy eruption after the acquisition of source tooth: the source of External apical root resorption 1/3
Tooth inherits permanent teeth dystopy eruption 12, and CV NR average time is 2.19 ± 0.18；The source tooth of External apical root resorption 1/2 inherits permanent teeth
Dystopy eruption 11, CV NR average time is 1.89 ± 0.13；The source tooth of External apical root resorption 2/3 inherits permanent teeth dystopy eruption 8
, CV NR average time is 1.42 ± 0.12, and root of the tooth fully absorbs but the complete source tooth of pulp chamber bottom is inherited permanent teeth dystopy and sprouted
2 out, CV NR average time is 1.03 ± 0.15.
The study result show that: the deciduous teeth to fall off naturally are the tooth without dental pulp, no stem cell；Deciduous teeth are in II degree
Within loosening, 2/3 or more External apical root resorption or root of the tooth fully absorb but pulp chamber bottom it is complete in the presence of, dental pulp stem cell extracts
Success rate is high, and on the eruption of permanent teeth without too big influence, acquires opportunity for optimal deciduous teeth source tooth.The present invention is clinical from now on newborn
The acquisition opportunity of sound of baby talk marrow stem cell source tooth provides fundamental basis.
Specific embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Quantitative test in following embodiments, is respectively provided with three repeated experiments, and results are averaged.
Deciduous tooth retention in following embodiments, which refers to, inherits permanent teeth eruption, fails the deciduous teeth to fall off on time；Or it is permanent
The non-eruption of tooth, the deciduous teeth being retained in permanent dentition.
Source tooth in following embodiments refers to the healthy deciduous teeth or perseverance pulled out to extract the seed cell of oral cavity stem cell
Tooth or wisdom tooth (best age of source tooth acquisition 6-20 years old).
Tooth is divided into following three kinds according to aeration level: once loosening (I degree), two degree of loosenings (II degree) and three degree of loosenings
(III degree).Wherein, it once loosens (I degree) and refers to the dynamic degree in tooth cheek tongue direction (inward-outward direction) within 1mm, other directions do not have
There is degree；Two degree loosen situation there are two types of (II degree): one is the dynamic degree in tooth cheek tongue direction (inward-outward direction) between 1-2mm,
Two be that there are cheek tongue direction (inward-outward direction) and the dynamic degree of near and far direction (left and right directions) both direction for tooth；III degree of tooth pine
Dynamic also there are two types of situations: one for the dynamic degree in tooth cheek tongue direction (inward-outward direction) is more than 2mm, and two be that there are cheek tongue directions for tooth
The dynamic degree of (inward-outward direction), near and far direction (left and right directions) and three directions of vertical direction (up and down direction).
Embodiment 1, using deciduous teeth as the screening of source tooth and extraction
One, the screening of deciduous teeth
1, screening criteria
The deciduous tooth retention situation of 173 pupils is defined by clapping tip of a root X-ray film or curved surface laminagram.The result shows that: 173
The deciduous tooth retention situation of example pupil meets the diagnostic criteria in " pedodontics " about deciduous tooth retention, and all deciduous teeth
It is the deciduous teeth bad without dental caries of health, close to replacement period, permanent teeth has been approached eruption or eruption.
It is loosened according to deciduous teeth and the deciduous teeth of selection is divided into following five kinds by the difference of External apical root resorption situation:
A) I degree of loosening, source tooth are deciduous tooth retention, External apical root resorption 1/3；
B) I degree or the loosening of II degree, source tooth are deciduous tooth retention, External apical root resorption 1/2；
C) II degree loosens, and source tooth is deciduous tooth retention, External apical root resorption 2/3；
D) II degree or III degree of loosening, source tooth are deciduous tooth retention, and root of the tooth fully absorbs but pulp chamber bottom completely exists；
E) III degree of loosening, source tooth are the deciduous teeth that nature falls off.
2, instrument and drug
(1) instrument prepares
(the sensible sharp medical instrument trade in the oral cavity single tooth anesthesia painless anesthesia injection instrument Dalian has STA
Limit company)；Dedicated tooth pulling surgery packet: mouth mirror, tweezers, is suitble to suffer from the dental forceps of tooth, dental elevator, excavator spoon, sterilization hole towel, apply probe
Material, periosteum devator (spare).
(2) drug prepares
UItracare lidocaine hydrochloride table fiber crops cream (white teeth company, the U.S.), Articaine epinephrine injection (France
Bi Lan company), the collection tube (Beijing Tai Sheng Biotechnology Co., Ltd) containing collection liquid.
Two, the acquisition of source tooth and Transfer method
(1) acquisition method of source tooth
1, it pulls out and takes out corresponding collection tube (deciduous teeth use thin collection tube) before tooth from refrigerator, be careful not to unscrew or pine
Dynamic lid.
2, before pulling out tooth, contained with the thimerosals such as the Iodophor wiping gargles such as corona or compound Chlorhexidine and gargled.
3, according to the conventional extraction method recorded in " Oral And Maxillofacial Surgery the 7th edition, People's Health Publisher publish " into
Row source tooth is pulled out.
4, collection tube lid is unscrewed after source tooth is pulled out rapidly, is put into collection tube in 7 seconds, screws on collection tube lid rapidly, it is ensured that
It tightens, is marked on collection tube after having collected, need to paste bar code on collection tube, fill in visitor using low temperature marking pen
The information such as family name, acquisition date, and avoid turning upside down and mix collecting pipe collection tube.
5, the collection tube equipped with collection liquid and tooth is put into 2-4 DEG C of refrigerator at once carry out it is stored refrigerated.
6, it fills in " GSP acquisition executes record " and pastes bar code, which need to be by doctor and relevant operation personnel
It fills in jointly and carries out signature confirmation.
(2) Transfer method of source tooth
The source tooth of acquisition is transported by Cold Chain Logistics, and transport temperature is maintained at 2-4 DEG C and (places under the conditions of 2-4 DEG C and do not surpass
It spends 72 hours and does not make significant difference to stem cell in success extraction source tooth).
It is intact whether per-trip inspection collection tube seals, and whether there is or not leakages, and takes in Beijing oral cavity stem cell bank logistics personnel
" GSP acquisition executes record " is filled in when walking source tooth, records time and means of transportation.During transportation, according to temperature measuring set
The temperature change of record all the time, is monitored and records to from the whole flow process for collecting storage.
Three, deciduous teeth External apical root resorption situation extracts deciduous teeth dental pulp stem cell and deciduous teeth pull out the influence of rear CV NR situation
1, dental pulp stem cell be separately cultured and success rate of extracting
Dental pulp stem cell in the tooth of source is separately cultured using the routine techniques of culture mescenchymal stem cell, and at 20 days
Whether left and right confirmation dental pulp stem cell is successfully separated and cultivates amplification, counts success rate of extracting.The specific preparation of dental pulp stem cell,
Culture and identification method reference literature " SHED:Stem cells from human exfoliated deciduous
Step in 2003 vol.100:5807-5812 of teeth.Miura et.al, PNAS ".As a result with 13.0 software of SPSS into
Row statistical analysis, enumeration data is indicated with n%, uses X2It examines, is that difference has statistical significance with P < 0.05.
Testing result is as shown in table 1.As can be seen from the table: 32, the source tooth of External apical root resorption 1/3, dental pulp stem cell extract
Success 30, success rate 93.75%；35, the source tooth of External apical root resorption 1/2, dental pulp stem cell extract successfully 32, success rate
It is 91.42%；59, the source tooth of External apical root resorption 2/3, dental pulp stem cell extract successfully 54, success rate 91.53%；Root of the tooth is complete
Hypersorption but 37, the complete source tooth of pulp chamber bottom, dental pulp stem cell extracts successfully 34, success rate 91.89%；Naturally it falls off
Deciduous teeth 10, dental pulp stem cell extracts successfully 0.The each group tooth that External apical root resorption 1/3,1/2,2/3 and root of the tooth fully absorb
Marrow stem cell extracts successful percentage and is compared, P > 0.05, and no significance difference is anisotropic；Above four groups with the cream that falls off naturally
Tooth group is compared, P < 0.05, property that there were significant differences.Illustrate that the deciduous teeth that nature falls off are not suitable for carrying out deciduous teeth dental pulp stem cell
It extracts, other four groups of deciduous teeth dental pulp stem cell success rate of extracting reach 90% or more.
Table 1, deciduous teeth External apical root resorption situation and deciduous teeth dental pulp stem cell extract the case where result
| External apical root resorption degree | Source number of teeth amount | Extract successfully quantity | Success rate of extracting (percentage) |
| External apical root resorption 1/3 | 32 | 30 | 93.75% |
| External apical root resorption 1/2 | 35 | 32 | 91.42% |
| External apical root resorption 2/3 | 59 | 54 | 91.52% |
| Root of the tooth fully absorbs | 37 | 34 | 91.89% |
| Naturally the deciduous teeth to fall off | 10 | 0 | 0* |
2, deciduous teeth pull out the case where rear CV NR
After deciduous teeth are extracted, whether observation CV NR situation has exception, and counts CV NR abnormal (permanent teeth dystopy is sprouted
Number of cases and CV NR average time out).
The results are shown in Table 2.As can be seen from the table: the CV NR that 1/3,1/2 and 2/3 3 group of External apical root resorption is average
It is compared between time, P > 0.05, no significance difference is anisotropic；The above three groups creams for fully absorbing group with root of the tooth and falling off naturally
Tooth group compares, P < 0.05, property that there were significant differences.After from deciduous teeth source, tooth is pulled out, inherit the CV NR time the results show that cream
Tooth External apical root resorption is better, and it is faster that CV NR is inherited after pulling out.
The case where table 2, deciduous teeth External apical root resorption situation and deciduous teeth pull out rear CV NR
| External apical root resorption degree | CV NR is abnormal (number of cases) | CV NR average time (moon) |
| Root absorbs 1/3 | 12 | 2.19±0.18* |
| Root absorbs 1/2 | 11 | 1.89±0.13* |
| Root absorbs 2/3 | 8 | 1.42±0.12* |
| Root fully absorbs | 2 | 1.03±0.15 |
| Naturally the deciduous teeth to fall off | 4 | 0.65±0.23 |
In summary the case where success rate of deciduous teeth dental pulp stem cell extraction culture and deciduous teeth pull out rear CV NR, discovery
For the acquisition of deciduous teeth dental pulp stem cell source tooth, for deciduous teeth within the loosening of II degree, 2/3 or more External apical root resorption or root of the tooth are complete
Absorb but pulp chamber bottom it is complete in the presence of, deciduous teeth dental pulp stem cell success rate of extracting is high, and to the eruption of permanent teeth without too big
It influences, acquires opportunity for optimal deciduous teeth source tooth.
Embodiment 2, using permanent teeth as the screening of source tooth and extraction
One, the screening of permanent teeth
1, screening criteria
It is divided into following each group according to the dental caries bad degree of permanent teeth, with the presence or absence of obturator and growing state:
(1) source tooth is permanent teeth, and no dental caries are bad, can directly carry out source tooth acquisition without obturation for optimal teeth source.
(2) source tooth is permanent teeth, has dental caries bad, it is proposed that shooting Dental X-ray film judges former dental caries bad depth.If former dental caries are badly shallow dental caries
Or middle dental caries, it carries out pulp vitality and tests active, the acquisition of progress source tooth after removal dental caries bad tissue；If being badly deep dental caries if original dental caries not
The tooth acquisition of carry out source.
(3) source tooth is permanent teeth, and there are obturations, it is proposed that shooting Dental X-ray film judges former dental caries bad depth.If former dental caries are badly
Shallow dental caries or middle dental caries carry out pulp vitality and test active, the acquisition of progress source tooth after removal dental caries bad tissue；If former dental caries are badly deep dental caries
Then acquired without source tooth.
(4) source tooth is accessory tooth, it is proposed that shooting Dental X-ray film judgement with the presence of pulp cavity, carry out pulp vitality test it is active into
The acquisition of row source tooth.
(5) source tooth is Impacted Mandibular Wisdom, is mentioned if destroying the tissues of tooth such as corona without stem cell when source tooth acquires
It takes.
2, instrument and drug
With the instrument and drug in embodiment 1.
Two, the acquisition of source tooth and Transfer method
1, corresponding collection tube (permanent teeth, wisdom tooth are using thick collection tube) is taken out from refrigerator before pulling out tooth, is careful not to twist
Open or loosen lid.
2, before pulling out tooth, contained with the thimerosals such as the Iodophor wiping gargles such as corona or compound Chlorhexidine and gargled.
3, according to the conventional extraction method recorded in " Oral And Maxillofacial Surgery the 7th edition, People's Health Publisher publish " into
Row source tooth is pulled out.
4, collection tube lid is unscrewed after source tooth is pulled out rapidly, is put into collection tube in 7 seconds, screws on collection tube lid rapidly, it is ensured that
It tightens, is marked on collection tube after having acquired, need to paste bar code on collection tube, fill in visitor using low temperature marking pen
The information such as family name, acquisition date, and avoid turning upside down and mix collection tube.
5, the collection tube equipped with collection liquid and tooth is put into 2-4 DEG C of refrigerator at once carry out it is stored refrigerated.
6, it fills in " GSP acquisition executes record " and pastes bar code, which need to be by doctor and relevant operation personnel
It fills in jointly and carries out signature confirmation.
(2) Transfer method of source tooth
The source tooth of acquisition is transported by Cold Chain Logistics, and transport temperature is maintained at 2-4 DEG C and (places under the conditions of 2-4 DEG C and do not surpass
It spends 72 hours and does not make significant difference to stem cell in success extraction source tooth).
It is intact whether per-trip inspection collection tube seals, and whether there is or not leakages, and takes in Beijing oral cavity stem cell bank logistics personnel
" GSP acquisition executes record " is filled in when walking source tooth, records time and means of transportation.During transportation, according to temperature measuring set
The temperature change of record all the time, is monitored and records to from the whole flow process for collecting storage.
Three, dental pulp stem cell be separately cultured and success rate of extracting
Dental pulp stem cell in the tooth of source is separately cultured using the routine techniques of culture mescenchymal stem cell, and at 20 days
Whether left and right confirmation dental pulp stem cell is successfully separated and cultivates amplification, counts success rate of extracting.The specific preparation of dental pulp stem cell,
Culture and identification method reference literature " SHED:Stem cells from human exfoliated deciduous
Step in 2003 vol.100:5807-5812 of teeth.Miura et.al, PNAS ".As a result with 13.0 software of SPSS into
Row statistical analysis, enumeration data is indicated with n%, uses X2It examines, is that difference has statistical significance with P < 0.05.
The result shows that: the success rate of extracting of permanent teeth dental pulp stem cell of the invention is 96%.
Claims (10)
1. source tooth is as the application in the raw material for extracting or preparing dental pulp stem cell；
The source tooth meet it is following 1) and 2) described in condition:
1) within II degree loosens；
2) it is gone bad without dental caries or dental caries bad degree is below middle dental caries.
2. source tooth is extracting or is preparing the application in dental pulp stem cell.
The source tooth meet it is following 1) and 2) described in condition:
1) within II degree loosens；
2) it is gone bad without dental caries or dental caries bad degree is below middle dental caries.
3. application according to claim 1 or 2, it is characterised in that:
The source tooth is deciduous teeth；
At least one of or, the source tooth is following a1)-a5):
A1) the source tooth of the loosening of I degree and External apical root resorption 1/3；
A2) the source tooth of the loosening of I degree and External apical root resorption 1/2；
A3) the source tooth of the loosening of II degree and External apical root resorption 1/2；
A4) the source tooth of the loosening of II degree and External apical root resorption 2/3；
A5) II degree loosens and root of the tooth fully absorbs but the complete existing source tooth of pulp chamber bottom；
Or, the source tooth is that II degree loosens and the deciduous teeth or II degree of External apical root resorption 2/3 loosen and root of the tooth fully absorbs but pulp chamber bottom
Complete existing deciduous teeth.
4. application according to claim 1 or 2, it is characterised in that:
The source tooth is permanent teeth or wisdom tooth, and when source tooth is permanent teeth or wisdom tooth, the source tooth meets in claims 1 or 2 2) institute
The condition stated.
5. application according to any one of claims 1-4, it is characterised in that:
The source tooth is saved or is transported at a temperature of (2-4) DEG C after acquisition；
Or, the source tooth after acquisition be built within 10 seconds containing save liquid container in；
Or, further include the steps that carrying out disinfection to source tooth appearance with disinfectant before the source tooth acquisition, the method tool of the disinfection
Body can be gargled to be wiped corona with thimerosal or being contained with gargle.
6. meeting the following 1) source with the 2) condition including choosing for the screening technique for the source tooth that dental pulp stem cell extracts
Tooth:
1) within II degree loosens；
2) it is gone bad without dental caries or dental caries bad degree is below middle dental caries.
7. according to the method described in claim 6, it is characterized by:
The source tooth is deciduous teeth；
At least one of or, the source tooth is following a1)-a5):
A1) the source tooth of the loosening of I degree and External apical root resorption 1/3；
A2) the source tooth of the loosening of I degree and External apical root resorption 1/2；
A3) the source tooth of the loosening of II degree and External apical root resorption 1/2；
A4) the source tooth of the loosening of II degree and External apical root resorption 2/3；
A5) II degree loosens and root of the tooth fully absorbs but the complete existing source tooth of pulp chamber bottom；
Or, the source tooth is that II degree loosens and the deciduous teeth or II degree of External apical root resorption 2/3 loosen and root of the tooth fully absorbs but pulp chamber bottom
Complete existing deciduous teeth.
8. according to the method described in claim 6, it is characterized by:
The source tooth is permanent teeth or wisdom tooth, when source tooth is permanent teeth or wisdom tooth, the source tooth meet in claim 6 2) described in
Condition.
9. according to the method any in claim 6-8, it is characterised in that:
The source tooth is saved or is transported at a temperature of (2-4) DEG C after acquisition；
Or, the source tooth after acquisition be built within 10 seconds containing save liquid container in；
Or, further include the steps that carrying out disinfection to source tooth appearance with disinfectant before the source tooth acquisition, the method tool of the disinfection
Body can be gargled to be wiped corona with thimerosal or being contained with gargle.
10. the application in dental pulp stem cell is being extracted or prepared to any method in claim 6-9.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070258957A1 (en) * | 2006-05-04 | 2007-11-08 | Russell Bowermaster | Method for obtaining and storing multipotent stem cells |
| CN104560872A (en) * | 2014-12-29 | 2015-04-29 | 深圳市北科生物科技有限公司 | In-vitro separation and cultivation method for tooth-sourced mesenchymal stem cells |
| CN106620763A (en) * | 2016-09-30 | 2017-05-10 | 广州赛莱拉干细胞科技股份有限公司 | Method for sterilizing tooth sample |
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2017
- 2017-11-14 CN CN201711120603.5A patent/CN109777769A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070258957A1 (en) * | 2006-05-04 | 2007-11-08 | Russell Bowermaster | Method for obtaining and storing multipotent stem cells |
| CN104560872A (en) * | 2014-12-29 | 2015-04-29 | 深圳市北科生物科技有限公司 | In-vitro separation and cultivation method for tooth-sourced mesenchymal stem cells |
| CN106620763A (en) * | 2016-09-30 | 2017-05-10 | 广州赛莱拉干细胞科技股份有限公司 | Method for sterilizing tooth sample |
Non-Patent Citations (5)
| Title |
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| LISIANE等: "The Isolation of Stem Cells from Human Deciduous Teeth Pulp Is Related to the Physiological Process of Resorption", 《JOURNAL OF ENDODONTICS》 * |
| 于兆兰等: "《临床口腔思维实践》", 31 July 2017, 天津科学技术出版 * |
| 胡祖斌等: "《妈妈宝宝口腔保健》", 31 December 2013, 武汉：湖北科学技术出版社 * |
| 路博闻等: "人脱落乳牙牙髓干细胞与人恒牙牙髓干细胞成骨分化及破骨能力的差异", 《J SOUTH MED UNIV》 * |
| 陆家瑜: "人乳牙牙髓干细胞培养、诱导及鉴定的实验研究", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
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