CN109777743A - Entomogenous fungi bacterial strain SB009 and its application to the high pathogenecity of Bemisia tabaci - Google Patents
Entomogenous fungi bacterial strain SB009 and its application to the high pathogenecity of Bemisia tabaci Download PDFInfo
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Abstract
The present invention relates to field of biological control more particularly to a kind of pair of Bemisia tabaci to have pathogenic strain excellent, be beauveria bassiana (Beauveria bassiana) bacterial strain SB009, it is preserved in Guangdong Province's Culture Collection (GDMCC), deposit number is GDMCC NO:60588.The present invention passes through reasonable experimental program, the index comprehensive screened in conjunction with strain excellent considers, the bacterial strain that there is excellent pathogenicity to Bemisia tabaci is screened, pathogenicity compares the bacterial strain that other strains testeds will be more stable, and the biological control of Bemisia tabaci is used for larger potentiality.
Description
Technical field
The present invention relates to plant protection arts, and in particular to the biological control of field of biological control more particularly to Bemisia tabaci
In.
Background technique
Chemical pesticide plays great function in control of insect, and the usage amount and yield of China's pesticide occupy the world for a long time
It is the first.However according to statistics, the effective rate of utilization of global pesticide is less than 30%, and the annual chemical pesticide usable floor area in China is 2.8 hundred million
hm2More than, amount of application reaches ten thousand tons of 50-60, and about 80% chemical pesticide is directly entered environment, and (Li Liang, Zhang Zhanying, Sun Huiying wait
Pesticide harm and environment protective plant protecting Discussion on Technology [J] Hubei plant protection, 2018, (4): 63-64).As chemical pesticide is a large amount of for a long time
It uses, bring " 3R " problem, that is, pest resistance to insecticide (Resistance), pesticide residue (Residue) and pest run wild again
(Resurgence) and problem of environmental pollution is increasingly severe.Therefore, to change using the tradition prevention and treatment based on chemical pesticide
Mode implements the plant protection policy of " putting prevention first, integrated control ", adheres to based on cultural control, based on biological control, and combine
Be extremely important (Yang Yunhua, Du Kai are developed in the sustainable Jiankang that other control measures carry out comprehensive treatment to pest
Book, Research Progress on Biological Control [J] Henan Science and Technology College journal of Shi Mingwang entomogenous fungi, 2011,39 (1): 34-37).
Entomogenous fungi is more with type, it is safe and effective, using the phase it is long, do not injure natural enemy, be not easy to produce resistance and can be quickly big
The advantages that amount production, is famous, there is unrivaled infectivity repeatedly and production convenience in biological control.What document was recorded
Entomogenous fungi has more than 100 a category, 800 kinds, it is known that entomogenous fungi mainly include Cordyceps sinensis fungus, Nemotophagous Fungi and parasitism
Nematode fungi, and constantly there are some novel species to be found.Reported about 405 kinds of China, wherein 215 kinds of the fungi of parasitic insect
(Li Yue, Jiang Chunjie, Zhao Yuying, wait entomogenous fungi in forestry pest biological control application [J] plant protection, 2016,10
(20):10-24).Entomogenous fungi be crop pests important pathogenic bacteria and be distributed widely in nature, according to investigations, field is got over
About 60% is caused by fungi in winter insect disease, and entomogenous fungi is strictly that the important factor of insect population natural contral and pest prevent
The important materials controlled have consequence in various forestry ecosystems, for maintaining the balance of the ecosystem to play not
Can or scarce effect, be IPM system important component (Molecular Identification of Qin Li green muscardine fungus and the anti-locust bacterial strain of muscardine and
Indoor Pathogenic Tests [D] Agricultural University Of Anhui, 2013).
Entomogenous fungi has century-old research history, especially relatively deep, relatively wide in the basic research of European and American countries entomogenous fungi.
1843, Bassi test, which demonstrates muscardine (Beauveria bassiana), can make silkworm disease popular in method, Yi Deng state, first
The microorganism cause of disease theory of insect disease has been created, and has established the basis of insect pathology, this is also what entomogenous fungi was studied
Really start；Metch-nikoff is in 1879-1880 using green muscardine fungus (Metarhizium anisopliae) prevention and treatment ground difficult to understand
Sharp scarab beetle (Anisoplara austriace), has opened the prelude of fungus control pest；Forbse etc. was once applied in the U.S.
Beauveria bassiana prevents and treats China bug fairly largely；In the 20-30 age in 19th century, Russia Metalnikvo, U.S. Wallenger
Et al. to muscardine, aspergillus flavus and green muscardine fungus prevention and treatment corn borer (Ostrinia nubilalis) it is extremely interested；Lefebver from
China northeast introduces the muscardine progress interior for infecting corn borer and field trial, and achieves progress (Li Zengzhi entomogenous fungi
In pests control apply status [J] Journal of Anhui Agricultural College, 1987, (2): 59-66)；China is since the 1950s
Using the large-scale eating-core bean worm of muscardine and corn borer, remarkable result is achieved；Entomogenous fungi constantly it is expanded, but
When moving towards sluggish, that time in 1956, the impact of organophosphorus pesticide is received, various biological control research are all put
It sets, wherein just including entomogenous fungi；Japan two fungal cultures be also forced bankruptcy, when DDT abuse cause disruption of ecological balance,
A series of problems, such as destruction of natural enemy injury, the drug resistance of pest, environment, human health；In addition, the plant cotton industry in America, also because
Pesticide abuse makes pest resistance enhancing lead to the massive losses of economic benefit, returns to the research of entomogenous fungi again
The public visual field (research of development [C] China entomogenous fungi and application that Li Zengzhi entomomycete is learned, 1996)；70 years 20th century
In generation, obtains success using Masscn Pine Caserpillar Control with Beauveria bassiana.China possesses maximum entomogenous fungi studying team in the world；Xu Qing
Feng professor, Li Zengzhi professor, Fan Meizhen professor and professor Chen Zhuan etc. are driving China's Resources of Entomogenous Fungi and application study side
Face played an important role.It succeeds as Russia, beauty, Deng state carry out biological control using entomogenous fungi, research worm is raw
Fungi is not only advantageous to pest control, but also suffers from terms of the production to people, life and the protection of environment extremely important
Function and significance reach entomogenous fungi research in addition, chemical pesticide control agriculture and forestry pest is destroyed caused by ecological environment
To the prosperity period.
Bemisia tabaci Bemisia tabaci (Gennadius) belongs to Semiptera (Homoptera) Aleyrodidae
(Aleyrodidae) small Aleyrodes are that the torrid zone, subtropical zone and adjacent temperate zone are regional, host's popularity most important in the world
One of agricultural pests can damage (Wu Qianxing, Liu Yong, Zhang You to crop by direct piercing and sucking and its effective transmitted virus
Army waits Bemisia tabaci that medicament resistance monitoring is often used to study [J] plant protection, 2018,2 (1): 119-120).From 90 years 20th century
Since the middle and later periods, which has been increasingly becoming China's important agricultural insect.Bemisia tabaci is divided into different bions, wherein Type B cigarette
The damaging range of aleyrodid is wide, the extent of injury is serious.Its adult and nymph directly pass through piercing and sucking water, lack plant nutrient
It is weary, and influence normal physiological activity.In addition, adult and nymph often secrete honeydew, Mei sewage disease, when insect density is high, leaf are induced
Black is presented in piece, seriously affects the exterior quality of photosynthesis and industrial crops；Adult height omnivorousness infects more than 1000 kinds of plants
Object, and propagate and come from Begomovirus (Begomovirus), Ampelo-virus (Crinivirus), carnation
Adelonosus (Carlavirus), tomato Tuo Laduo Tobamovirus (Torradovirus) and sweet potato viruses category (Ipomovirus)
More than 300 kinds of viruses.In the past 10 years, category member tomato yellow leaf curl virus Tomato yellow leaf curlvirus
(TYLCV) maximum to being endangered caused by China's agricultural.When cotton is compromised, there is moth patch in leaf front, when insect density is high
There is macula lutea in blocks, fruit abscission rate is caused when serious, influences output of cotton and fiber quality.
Bemisia tabaci Nymph possesses wax shell, and often fixation is adsorbed on crop surface, only with sucking mouth parts damage to crops, because
And increase difficulty of prevention and cure (Liu Zhao, Lei Zhongren to Bemisia tabaci have highly pathogenicity entomogenous fungi screen [J] China's Vegetable,
2014,1(3):37-40)；From just breaking out greatly in China over 2000 and distribution constantly expands, causes disaster, Important Economic is given to make
Object causes heavy losses (Chu D, Wan F H, Zhang Y J, et al.Change in the biotype
composition of Bemisia tabaci in Shandong Province of China from 2005 to 2008
[J].Environmental Entomology,2010,39(3):1028-1036).It is commonly used to the pest control with insecticide of prevention and treatment Bemisia tabaci
Agent is difficult to penetrate into it in vivo, often can only increase dose to reach control efficiency, not only increases cost in this way and also pollutes environment,
Even it can also develop drug resistance to pest.Therefore, the substitute technology for studying effectively preventing Bemisia tabaci seems especially important.
Insect pathogenic fungus not only has that significant epidemic potential, safe for human and animal and environment, pests are not easy to produce resistance
And have the convenience of production, preventing and treating Bemisia tabaci using it has great potential.Paecilomyces fumosoroseus is to nymph especially low age
The very high bacterium of nymph infection rate, and under control environment can in adult spread and epidemic and be usually used in the prevention and treatment of Bemisia tabaci.According to phase
Report is closed, entomogenous fungi has obtained good effect to the prevention and treatment of Bemisia tabaci Nymph on tomato.In addition, muscardine is to Bemisia tabaci
Nymph especially low instar nymphs also have biggish control potential, adjust Bemisia tabaci using the biological controls such as entomogenous fungi means
Density controls the harm of Bemisia tabaci, has caused the interest of numerous research workers.But it finds to the white of the high virulence of Bemisia tabaci
Stiff bacterium needs a large amount of collection muscardines and is screened.
Summary of the invention
In order to obtain the strain excellent of Bemisia tabaci biological control effect, 29 plants collected early period are primarily determined as muscardine
Bacterial strain, the present inventor determines its virulence to Bemisia tabaci 2 age nymph using leaf dipping method, if thus obtaining several plants of Bemisia tabacis
The pathogenic strain excellent of worm.
Wherein, experiment discovery do not belong to or pathogenicity of the bacterial strain of the same race to Bemisia tabaci all there may be differences, thus have
Different degrees of causative effect.Experimental result according to the present invention, in conjunction with the index comprehensive consideration that strain excellent screens, wherein
Pathogenicity is to measure the important indicator of fungal bacterial strain biological control potentiality, and the growth rate of bacterium colony or mycelia is then that evaluation is excellent
Another important evaluation index of insect pathogenic fungus, bacterial strain SB009 is 107Spore/mL and 108If to Bemisia tabaci under spore/mL
Worm has higher pathogenicity, and the pathogenicity of bacterial strain SB009 is more stable compared to other strains testeds, the bacterium colony of comprehensive bacterial strain
Growth rate considers that bacterial strain SB009 is the strain excellent that Bemisia tabaci Nymph causes a disease.
The bacterial strain is separated in soil under Sichuan Province Chengdu Qingyang District Wenshu Monastery tree, on PDA plate, 25
DEG C, 10 days, colony diameter 56.33mm, bacterium colony centre was velvet-like, and edge is powdered, superficial white, and back side crocus has a small amount of wrinkle
Pleat.Spore oval and subsphaeroidal, 2.095 μm of mycelia length, it is 1.524 μm wide.Identification is further verified by molecular biology,
Belong to beauveria bassiana (Beauveria bassiana).
Further, the application the present invention also provides SB009 bacterial strain in the biological control of Bemisia tabaci.
In the specific implementation, spore liquid can will be made after the strain culturing or containing the microbial inoculum of spore.Preferably, it is applying
The spore concentration of used time bacterial strain is 107Spore/mL to 109Spore/mL, most preferably 108Spore/mL.
Further, predict Bemisia tabaci will occur when or early period of origination application the microbial inoculum.
By reasonable experimental program, the index comprehensive screened in conjunction with strain excellent considers the present invention, screens to tobacco powder
Lice has a bacterial strain SB009 of excellent pathogenicity, and 10882.93%, and its pathogenicity are reached to Bemisia tabaci virulence under spore/mL
Bacterial strain that will be more stable compared to other strains testeds is (107It is also relatively high to Bemisia tabaci virulence under spore/mL), have larger
Potentiality are used for the biological control of Bemisia tabaci.
Wherein, bacterial strain SB009 was preserved in Guangdong Province's Culture Collection (GDMCC) on 2 21st, 2019,
Deposit number is GDMCC NO:60588.Bacterial strain SP433 was preserved in Guangdong Province's Culture Collection on 2 21st, 2019
(GDMCC), deposit number is GDMCC NO:60589.
Detailed description of the invention
Fig. 1 SB009 colonial morphology figure
Note: left figure and right figure are SB009 bacterium colony front elevation and back view
Fig. 2 SB009 spore shape figure
Pcr amplification product electrophoretogram in Fig. 3 Molecular Identification
Fig. 4 Molecular Phylogenetic tree
Specific embodiment
It is introduced, but is not constituted to limit of the invention below by R&D process and specific embodiment of the invention
System.
1 for trying host plant
Cotton " Lu Mianyan 32 " is purchased from seed control station, Weifang City, Shandong Province.Under natural conditions, with through autoclaved
Nutrient soil pot cultivated cotton seed, growth period cotton seedling is placed in clean dependent insect cage (60 × 60 × 60cm), to Miao Changzhi
For testing when the 6-8 leaf phase.
2 for trying insect
Pick up from Agricultural University Of South China's teaching practice farm within Type B Bemisia tabaci 2005, host plant is tomato.After adopting back
It is isolated raising successive propagation on the cotton host of biological control Ministry of Education Engineering Research Center.Rearing conditions are temperature (27
± 1) DEG C, photoperiod L14:D10, relative humidity (75 ± 5) %.
3 muscardines being screened for
The entomogenous fungi that this experiment is chosen is 29 plants total, is that inside is preserved in the biological control Ministry of Education, Agricultural University Of South China
The bacterial strain of Engineering Research Center succeeding preservation.Specifying information is detailed in following table.
1 strains tested of table
4 experimental methods
The preparation of 4.1 spore suspension liquors
On PDA plate after 26 ± 1 DEG C of culture 7d, 0.05% Tween-80 of entomogenous fungi conidium of spore is sufficiently produced
Sterile water elution spore is wiped with magnetic stirrer, then after being placed in 25 DEG C of shaken cultivation 25min of shaking table 180rpm with double-deck
The filtering of mirror paper, is counted with blood counting chamber, measures the concentration of mother liquor, be configured to 1 × 107Spore/mL and 1 × 108Spore/mL
Spore suspension.
Toxicity test of 4.2 entomogenous fungis to Bemisia tabaci 2 age nymph
The cotton leaf back side with 2 age of Bemisia tabaci nymph is dipped in the spore suspension prepared, is taken out after 30s, to
After leaf natural is dried, vacuum side of blade is placed in agar medium upward, Kong Tongqi is sealed and pricked with preservative film, by culture dish
Slant setting is placed in growth cabinet, rearing conditions be temperature (27 ± 1) DEG C, photoperiod L14:D10, relative humidity (75 ±
5) %.Using 0.05% Tween-80 sterile water as blank control.Each processing is repeated 4 times, 100 nymphs of each repetition.Respectively at
3d, 5d and 7d check the death condition of Bemisia tabaci and record death toll, calculate the death rate.4.3 data processing
Using SPSS17.0, Statistix 8 and Excel software, Duncan method is shown for data processing and statistical analysis
Work property is examined.
5 results and analysis
The experimental results showed that same concentration, the pathogenicity of 29 plants of entomogenous fungis is had differences, and the death rate of nymph is with place
It manages the increase of time and rises；Same time various concentration, bacterial strain is to the lethality of Bemisia tabaci 2 age nymph with spore concentration
Increase and increases.Concrete outcome is shown in Table 2 and table 3.
Table 21 × 107The death rate (%) of spore/mL spore suspension to Bemisia tabaci 2 age nymph
Note: examining through Duncan, and the different lowercases of same row indicate pathogenicity significant difference (P < between different strains
0.05)
Table 31 × 108The death rate (%) of spore/mL spore suspension to Bemisia tabaci 2 age nymph
Note: examining through Duncan, and the different lowercases of same row indicate pathogenicity significant difference (P < between different strains
0.05)
Wherein, when spore suspension concentration is 1 × 108When spore/ml (table 2), handle early period (3d), each bacterial strain it is lethal
Rate shows significant difference, and the lethality of bacterial strain is differed by 2.13% to 29.07%, bacterial strain SP665, SB015, SB009,
For the lethality of SB039 25% or more, lethality accounts for half less than 10% with the lethality of bacterial strain SP031 is minimum, only
2.13%；5d is handled, the lethality highest between bacterial strain can differ 4 times, there are significant difference between bacterial strain, bacterial strain SB015, SB063,
For the lethality of SP016, SP433, SB009, SB036, SB674 up to 50% or more, the highest of bacterial strain SB015 is 61.76%,
And the lethality of bacterial strain SB006, SB050, SB004, SP031, less than 30%, the lethality of bacterial strain SP031 is minimum, only
13.07%；7d is handled, the death rate of control group is only 13.3%, and relatively low, the difference of each bacterial strain lethality is reduced, and 27
The lethality of strain bacterial strain is in 59.5-82.93%.Wherein the lethality of bacterial strain SP433, SB009, SB050, SB036, SB043 reaches
The lethality highest of 80% or more, bacterial strain SP433 are taken second place also up to 87.37%, SB009 up to 82.93%, show that this 5 plants of worms are raw true
Bacterium has strong lethal effect thus to have prevention and treatment potentiality well to Bemisia tabaci, belongs to potential excellent biocontrol microorganisms.
But in conjunction with table 2 and table 3 it is found that same bacterial strain various concentration, each bacterial strain have certain control efficiency to Bemisia tabaci
And lethality increases in various degree with the increase of concentration, and bacterial strain SB039, SB063, SP031, SP670, SB671,
SB035 is 1 × 10 in concentration7Spore/ml and 1 × 108Similar lethality is obtained after handling Bemisia tabaci Nymph 7d under spore/ml,
If illustrating that these bacterial strains is selected to prevent and treat Bemisia tabaci, low concentration can reach good result；Bacterial strain SB043, SB050, SB036 are in spore
Sub- concentration is 1 × 107When spore/ml, pathogenicity is markedly less than other strains testeds, when concentration is 108When spore/ml, pathogenicity
But it is better than other bacterial strains, illustrates that these bacterial strains have with environment miospore concentration compared with Important Relations in terms of control of insect.Bacterial strain
SB039, SP433, SB063, SB009, SB674, SP670 are 1 × 10 in spore concentration7When spore/ml, lethality 65% with
On, bacterial strain SP433, SB009, SB050, SB036, SB043 are 1 × 10 in spore concentration8When spore/ml, lethality is up to 80%
More than, while also indicating that the pathogenicity of bacterial strain SB009 and SP433 are strong and stability is better than other strains testeds.
Control group is that 0.05% Tween-80 handles Bemisia tabaci Nymph, and lethality is very low；When with concentration be 108Spore/mL spore
When sub- suspension processing Bemisia tabaci Nymph, not only pathogenicity has differences each bacterial strain, but also the knockdown time that causes a disease is also inconsistent.Have
A little fungies belong to even same kind despite same, but the pathogenicity of bacterial strain can also have differences；Some bacterial strains are in tobacco powder
Larger difference is also shown in lice nymph lethal time, some short time effects are undesirable, but as time of infection extension is lethal
Rate increases, and some bacterial strain lethalities and knockdown time difference are little, illustrates feelings occur in conjunction with pest in the prevention and treatment of pest
Condition reasonably selects to use the time；In addition, spore concentration is also the Bemisia tabaci of some bacterial strains pair an important factor for influencing pathogenicity
The cumulative mortality of nymph is gradually increased with the increase of spore concentration, and then to show lethality poor with test concentrations for some bacterial strains
It is different little, it selects low concentration that can reach identical and infects effect.In conjunction with strain excellent screen index comprehensive consider, bacterium colony or
The growth rate of mycelia is to evaluate an important evaluation index of excellent insect pathogenic fungus, and pathogenicity is to measure fungal bacterial strain
The important indicator of biological control potentiality.
5 bacterial strain SB009 further verify qualification result
(1) acquisition of bacterial strain SB009
Bacterial strain SB009 was initially separated in soil under Sichuan Province Chengdu Qingyang District Wenshu Monastery tree in 2007, was
China domestic bacterial strain.
Acquire pedotheque, when sampling takes under surface soil soil 100g or so at 10~15cm, with taking back after packaging plastic bags
Laboratory treatment.Stone grain and sundries are removed in pedotheque sieving, and the Tween 80 for then taking pure land 10g to be suspended in 90mL 0.1% is molten
It in liquid, shakes up, stands 15min, take its supernatant 2mL to be diluted in 0.05% Tween 80 of 8mL, be formulated as Soil Slurry.It will
0.1mL Soil Slurry is inoculated on rose-bengal agar medium plate, is pushed away suspension with triangular glass sleaker even in flat
Under plate surface, then 25 DEG C of 3~7d of culture cut single colonie with oese, are inoculated on PDA plate and continue to cultivate, obtain ball spore
Muscardine bacterial strain SB009.The transplanting of mycelia block is cut in the inclined-plane PDA, continues to cultivate, is preserved in refrigerator at 4 DEG C.
(2) Morphological Identification
Bacterial strain SB009 is on PDA plate, and 25 DEG C, 10 days, colony diameter 56.33mm, velvet-like, the edge powder in bacterium colony centre
Shape, superficial white, back side crocus have a small amount of gauffer (Fig. 1).Spore oval and subsphaeroidal, 2.095 μm of mycelia length, it is wide
1.524 μm (Fig. 2).
(3) molecular biology identification
Molecular biology identification is carried out for 29 plants of bacterial strains.
DNA is extracted using CTAB method, comprising the following steps:
(a) Strain of Beauveria bassiana SB009 is cultivated after a week on PDA plate, carefully scrapes mycelium in mortar,
Liquid nitrogen is added sufficiently to grind rapidly；
(b) it takes the mycelium ground in right amount to be quickly transferred in centrifuge tube, the DNA that 300 μ L are preheated through 65 DEG C is added and extracts
Liquid lysate, mixes well, and isometric chloroform/isoamyl alcohol (24/1) mixed liquor is added and mixes, water-bath 1h, 10min at 65 DEG C
Gently concussion is primary for left and right, is centrifuged 5min under 12000rpm normal temperature condition；
(c) slowly it is mixed to be slowly added to isometric chloroform/isoamyl alcohol (24/1) in another new centrifuge tube for Aspirate supernatant
It closes liquid to be extracted again, is centrifuged 5min under 12000rpm normal temperature condition；
(d) slowly in another new centrifuge tube the sodium acetate of 1/10 volume is added, what is be pre-chilled in equal volume is different in Aspirate supernatant
Propyl alcohol, the static 15min of room temperature；
(e) 70% ethanol washing is added to precipitate 2 times；
(f) ethyl alcohol is slowly sucked out in liquid-transfering gun abutting tube wall, is placed in drying in superclean bench；
(g) gained precipitating is dissolved in the TE of 50 μ L；
(h) electrophoresis detection is carried out with 1% Ago-Gel, remaining extract saves under the conditions of being placed in -20 DEG C.
The PCR the primer of fungal DNA is the universal primer for Bloc gene: B5.1F:5 '-
Cgacccggccaactactttga-3 ' and B3.1R:5 '-gtcttccagtaccactacgcc-3 '.Amplification condition: 94 DEG C of pre- changes
Property 3min；94 DEG C of denaturation 30s, 55 DEG C of annealing 30s, 72 DEG C of extension 45s, totally 35 recycle；72 DEG C of extension 10min.
50 μ L reaction systems are as follows:
PCR product carries out 1% agarose gel electrophoresis (contain EB), and buffer is 1 × TAE, voltage 150V, electric current 220mA,
Marker DL2000 after electrophoresis, is detected and is photographed to record in gel imager as molecular weight marker.
Specific PCR products entrust Shanghai Major Biological Medical Technology Co., Ltd. to be sequenced.Sequence alignment and development
Tree building: the sequencing result higher correlated series of blast search homology, using the Cluster W in MEGA5.1 software into
Row sequence alignment and Multiple sequence alignments.And Neighbour-joining (NJ) phylogenetic tree is constructed in the software.
Qualification result
It (1), can be according to the migration distance of PCR product come quasi- as shown in figure 3, the electrophoretic band of each swimming lane is than more visible
The genetic fragment for really judging the bacterial strain is about 1500bp, wherein only show the bacterial strain of number 1 to 18, and number 4 is SB009 bacterium
Strain.
(2) by the Bloc sequence of the obtained strain genotype and the 3 plants of muscardine bacterium correlated series downloaded from GenBank
Combination constructs Molecular Phylogenetic tree, is the white deadlock of ball spore as a result as shown in figure 4, SB009 bacterial strain belongs to Beauveria bassiana
Bacterium.
Comprehensive morphological feature and physio-biochemical characteristics qualification result show that the bacterial strain belongs to ball spore beauveria, name
For SB009, and it was preserved in Guangdong Province's Culture Collection (GDMCC) on 2 21st, 2019, deposit number is
GDMCC NO:60588, preservation address are GuangZhou, China.
Claims (6)
1. a kind of pair of Bemisia tabaci has pathogenic strain excellent, be beauveria bassiana (Beauveria bassiana) bacterial strain
SB009 is preserved in Guangdong Province's Culture Collection (GDMCC), and deposit number is GDMCC NO:60588.
2. application of the bacterial strain as described in claim 1 in prevention and treatment Bemisia tabaci.
3. application as claimed in claim 2, which is characterized in that spore liquid will be made after the strain culturing, containing the bacterium of spore
Agent.
4. application as claimed in claim 2 or claim 3, which is characterized in that the spore concentration of bacterial strain is 10 when application7 Spore/mL is extremely
109 Spore/mL.
5. application as claimed in claim 2 or claim 3, which is characterized in that the spore concentration of bacterial strain is 10 when application8 Spore/mL.
6. application as claimed in claim 2 or claim 3, which is characterized in that when predicting that Bemisia tabaci will occur or early period of origination is applied
The microbial inoculum.
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| CN113142244A (en) * | 2021-01-05 | 2021-07-23 | 华南农业大学 | Synergistic prevention and treatment of common thrips by Beauveria bassiana SB063 and spinetoram |
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| CN113684136A (en) * | 2021-07-21 | 2021-11-23 | 华南农业大学 | Broad-spectrum insecticidal beauveria bassiana strain and application thereof |
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| CN111909854A (en) * | 2020-07-20 | 2020-11-10 | 华南农业大学 | Zero-valent iron-Beauveria brucei nano-particles and preparation method and application thereof |
| CN111909854B (en) * | 2020-07-20 | 2023-02-03 | 华南农业大学 | Zero-valent iron-Beauveria bassiana nano-particles and preparation method and application thereof |
| CN113142244A (en) * | 2021-01-05 | 2021-07-23 | 华南农业大学 | Synergistic prevention and treatment of common thrips by Beauveria bassiana SB063 and spinetoram |
| CN113142245A (en) * | 2021-01-05 | 2021-07-23 | 华南农业大学 | Synergistic prevention and treatment of common thrips by Beauveria bassiana SB038 and spinetoram |
| CN113142244B (en) * | 2021-01-05 | 2022-03-29 | 华南农业大学 | Synergistic prevention and treatment of common thrips by Beauveria bassiana SB063 and spinetoram |
| CN113142245B (en) * | 2021-01-05 | 2022-04-05 | 华南农业大学 | Synergistic prevention and treatment of common thrips by Beauveria bassiana SB038 and spinetoram |
| CN113684136A (en) * | 2021-07-21 | 2021-11-23 | 华南农业大学 | Broad-spectrum insecticidal beauveria bassiana strain and application thereof |
| CN113684136B (en) * | 2021-07-21 | 2023-08-22 | 华南农业大学 | Broad-spectrum insecticidal beauveria bassiana strain and application thereof |
| CN114292760A (en) * | 2022-01-30 | 2022-04-08 | 华南农业大学 | Spaceflight entomogenous fungus strain SCAUHT38 with high pathogenicity and high ultraviolet resistance to common thrips and application thereof |
| CN114292760B (en) * | 2022-01-30 | 2024-03-26 | 华南农业大学 | High pathogenicity and high ultraviolet resistance space insect fungus strain SCAUHT38 for common thrips and application thereof |
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