CN109777100A - A kind of preparation method of redox graphene-poly-dopamine-glutathione composite material - Google Patents
A kind of preparation method of redox graphene-poly-dopamine-glutathione composite material Download PDFInfo
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- CN109777100A CN109777100A CN201910124179.4A CN201910124179A CN109777100A CN 109777100 A CN109777100 A CN 109777100A CN 201910124179 A CN201910124179 A CN 201910124179A CN 109777100 A CN109777100 A CN 109777100A
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- 229960003180 glutathione Drugs 0.000 title claims abstract description 61
- 239000002131 composite material Substances 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- 229920001690 polydopamine Polymers 0.000 claims abstract description 43
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims abstract description 34
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 27
- 229910002804 graphite Inorganic materials 0.000 claims abstract description 18
- 239000010439 graphite Substances 0.000 claims abstract description 18
- 108010024636 Glutathione Proteins 0.000 claims abstract description 15
- NUJOXMJBOLGQSY-UHFFFAOYSA-N manganese dioxide Chemical compound O=[Mn]=O NUJOXMJBOLGQSY-UHFFFAOYSA-N 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000007853 buffer solution Substances 0.000 claims abstract description 9
- 239000000725 suspension Substances 0.000 claims abstract description 9
- 238000006243 chemical reaction Methods 0.000 claims abstract description 7
- 239000008367 deionised water Substances 0.000 claims abstract description 6
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims abstract description 6
- CTENFNNZBMHDDG-UHFFFAOYSA-N Dopamine hydrochloride Chemical compound Cl.NCCC1=CC=C(O)C(O)=C1 CTENFNNZBMHDDG-UHFFFAOYSA-N 0.000 claims abstract description 5
- 229960001149 dopamine hydrochloride Drugs 0.000 claims abstract description 5
- 239000007787 solid Substances 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 5
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 2
- 239000006228 supernatant Substances 0.000 claims description 2
- 239000003054 catalyst Substances 0.000 claims 2
- WTDRDQBEARUVNC-UHFFFAOYSA-N L-Dopa Natural products OC(=O)C(N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-UHFFFAOYSA-N 0.000 claims 1
- MHUWZNTUIIFHAS-CLFAGFIQSA-N dioleoyl phosphatidic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC(COP(O)(O)=O)OC(=O)CCCCCCC\C=C/CCCCCCCC MHUWZNTUIIFHAS-CLFAGFIQSA-N 0.000 claims 1
- 229960004502 levodopa Drugs 0.000 claims 1
- 239000000126 substance Substances 0.000 abstract description 5
- 238000012360 testing method Methods 0.000 abstract description 3
- 238000001914 filtration Methods 0.000 abstract 1
- 229910021389 graphene Inorganic materials 0.000 description 8
- VYFYYTLLBUKUHU-UHFFFAOYSA-N dopamine Chemical compound NCCC1=CC=C(O)C(O)=C1 VYFYYTLLBUKUHU-UHFFFAOYSA-N 0.000 description 7
- 239000000243 solution Substances 0.000 description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- 238000002484 cyclic voltammetry Methods 0.000 description 4
- 229910021397 glassy carbon Inorganic materials 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 229960003638 dopamine Drugs 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 238000007259 addition reaction Methods 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 2
- 235000018417 cysteine Nutrition 0.000 description 2
- 238000001548 drop coating Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000011056 performance test Methods 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 238000010408 sweeping Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- -1 Graphite alkene Chemical class 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 241001481789 Rupicapra Species 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 150000001721 carbon Chemical group 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 229910052593 corundum Inorganic materials 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- ZOMNIUBKTOKEHS-UHFFFAOYSA-L dimercury dichloride Chemical class Cl[Hg][Hg]Cl ZOMNIUBKTOKEHS-UHFFFAOYSA-L 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000005518 electrochemistry Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 239000005457 ice water Substances 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 238000003760 magnetic stirring Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 238000006396 nitration reaction Methods 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000012286 potassium permanganate Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 230000027756 respiratory electron transport chain Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 229910001845 yogo sapphire Inorganic materials 0.000 description 1
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- Carbon And Carbon Compounds (AREA)
Abstract
The present invention provides a kind of preparation methods of redox graphene-poly-dopamine-glutathione composite material, are that graphite oxide ultrasonic disperse is formed unit for uniform suspension in deionized water；Dopamine hydrochloride is dissolved in the Tris-Hcl buffer solution of pH=8.5, is then added in graphite oxide suspension, the then stirring 22 ~ for 24 hours at 60 DEG C ~ 65 DEG C stands, separates, washes, and it is dry, obtain redox graphene-poly-dopamine composite material；Continue for redox graphene-poly-dopamine to be dissolved in pH=8.5Tris-Hcl buffer solution, is ultrasonically treated 5 ~ 6h；Addition glutathione and manganese dioxide, room temperature reaction 22 ~ for 24 hours, filtering is dry to get black solid redox graphene-poly-dopamine-glutathione.The test result of chemical property shows that redox graphene-poly-dopamine-glutathione composite material prepared by the present invention has excellent electrical property.
Description
Technical field
The present invention relates to a kind of redox graphene-poly-dopamine-glutathione composite material with excellent properties
Preparation method, belong to technical field of composite materials.
Background technique
Redox graphene is by carbon atom with sp2The New Two Dimensional that the carbon monoatomic layer of hybrid form connection is constituted is former
Sub- crystal, basic structural unit are most stable of benzene hexatomic rings in organic material, are two-dimension nano materials most thin at present,
Thickness is only 0.35nm.Redox graphene has impayable high electron mobility, and electronics is in graphene molecules
Electron transfer rate is up to 15000cm2.V-1.s-1, and the mobility of silicon only has 1400cm2.V-1.s-1.However, single oxygen reduction
Graphite alkene make it using narrow, therefore synthesizing to restore petrochemical industry graphene is that the composite material of base material has more
Good performance and application.
Summary of the invention
The object of the present invention is to provide a kind of preparations of redox graphene-poly-dopamine-glutathione composite material
Method.
The present invention simultaneously to prepared redox graphene-poly-dopamine-glutathione composite material structure and
Electrochemistry can be carried out characterization and test.
One, redox graphene-poly-dopamine-glutathione composite material preparation
The preparation method of redox graphene-poly-dopamine-glutathione composite material of the present invention, including following technique step
It is rapid:
(1) redox graphene-poly-dopamine preparation: graphite oxide ultrasonic disperse is formed in deionized water uniformly outstanding
Supernatant liquid；Dopamine hydrochloride is dissolved in Tris-HCl(pH=8.5) buffer solution in, be added in graphite oxide suspension, mix
The stirring 22 ~ for 24 hours at 60 DEG C ~ 65 DEG C, stands, separates, wash after closing uniformly, dry, obtains redox graphene-poly-dopamine
Composite material is labeled as rGO-PDA.Wherein graphite oxide and the mass ratio of poly-dopamine are 1:1 ~ 2:1.
(2) redox graphene-poly-dopamine-glutathione composite material preparation: by above-mentioned reduction-oxidation graphite
Alkene-poly-dopamine is dissolved in Tris-HCl(pH=8.5) buffer solution in, be ultrasonically treated 5 ~ 6h；Add glutathione and catalysis
Agent manganese dioxide, room temperature reaction 22 ~ for 24 hours；It filters after the reaction was completed, it is dry, it is poly- more to obtain black solid redox graphene-
Bar amine-glutathione is labeled as rGO-PDA-GSH.Wherein, the quality of redox graphene-poly-dopamine and glutathione
Than for 1:1 ~ 1:2.
In order to promote sulfydryl on glutathione and poly-dopamine that addition reaction occurs well, and connect glutathione
Onto poly-dopamine, the additional amount of manganese dioxide be redox graphene-poly-dopamine, glutathione total amount 10% ~ 15%.
The drying is dry 8 ~ 10h in freeze drying box.
Two, redox graphene-poly-dopamine-glutathione composite material structure
Fig. 1 is the scanning electron microscope (SEM) photograph of redox graphene-poly-dopamine-glutathione composite material prepared by the present invention.From
Fig. 1 can be seen that redox graphene-poly-dopamine-glutathione with three-dimensional structure, regular appearance, distribution of pores
Uniformly, and it can see the spherical structure of poly-dopamine, this also illustrates that the preparation of composite material is successful.
Three, redox graphene-poly-dopamine-glutathione composite material chemical property
The test of chemical property: bare glassy carbon electrode uses 1 μm, 0.3 μm and 0.05 μm of Al respectively2O3Suspension is polished,
Respectively in two bare glassy carbon electrode surface drop coatings rGO-PDA, rGO-PDA-GSH, it is prepared into electrode rGO-PDA/GCE, rGO-
PDA-GSH/GCE.Then it is respectively placed in 5.0mM [Fe (CN)6]4-/3-Solution (KCl comprising 0.1M) is 50mV/s sweeping speed,
Scanning range -0.2V ~ 0.6V condition carries out its electric conductivity of cyclic voltammetry.
Fig. 2 is the cyclic voltammetry curve of rGO-PDA/GCE, rGO-PDA-GSH/GCE.From fig. 2 it can be seen that rGO-
The peak current of PDA-GSH can be apparently higher than 2 times that rGO-PDA(is almost rGO-PDA), this illustrates that redox graphene-is poly-
Dopamine-glutathione composite material has excellent electrical property.
The principle of the present invention: dopamine, its chemical name is 4- (2- amino-ethyl) -1,2- benzenediols, in specific pH
Under condition or oxidizing condition, the phenolic hydroxyl group of dopamine is easily oxidized to quinone, and then finally aggregates into poly-dopamine material.Due to
Poly-dopamine material surface has functional group (- OH ,-NH abundant2), it almost can be compound with any material progress, and have pole
Good biocompatibility and biodegradability.Glutathione (glutathione, GSH) is by glutamic acid, cysteine and sweet
Propylhomoserin combines, and is a kind of tripeptides containing γ-amido bond and sulfydryl, has antioxidation and integrates detoxication.Cysteine
On sulfydryl be glutathione active group, the sulfydryl on glutathione can and poly-dopamine addition reaction occurs so that paddy Guang
Sweet peptide can be good at being connected to above redox graphene by poly-dopamine, make redox graphene, dopamine, paddy
The sweet peptide collaboration of Guang, composite material obtained have excellent chemical property.
Detailed description of the invention
Fig. 1 is redox graphene-poly-dopamine-glutathione composite material scanning electron microscope (SEM) photograph.
Fig. 2 is redox graphene-poly-dopamine-glutathione composite material cyclic voltammetry curve.
Specific embodiment
Below by specific example to redox graphene-poly-dopamine of the present invention-glutathione composite material rGO-
Preparation, performance of PDA-GSH etc. are described further.
Embodiment 1
(1) preparation of graphite oxide: the process of first step pre-oxidation: under magnetic stirring by 1.25gK2S2O8And 1.25gP2O5
It is added at one time the dense H of 12.5mL2SO4In, 1g graphite powder is then added, mixed solution will be changed and be heated to 80 DEG C, stirred in magnetic force
It mixes down and is heated to reflux 5h, after cooling, the dilution of 200mL water, the neutrality being then filtered, washed, in 60 DEG C of vacuum are added into solution
Under the conditions of it is dry.The further oxidation process of second step: the graphite oxide of above-mentioned drying is weighed 1g, is scattered under condition of ice bath
In the nitration mixture (volume ratio of the concentrated sulfuric acid and phosphoric acid is 3:1) of the 120mL concentrated sulfuric acid and phosphoric acid, temperature is kept to be 0 ~ 5 DEG C and constantly stir
Lower addition 9g potassium permanganate is mixed, temperature is then risen into 50 DEG C of stirring 12h.System temperature is cooled to room temperature, is separately added into
The hydrogen peroxide of 200mL ice water 5mL30%, and be stirred continuously, the HCl of 5mL5% is added, is finally washed, filters, is dried to obtain
Graphite oxide rGO；
(2) redox graphene-poly-dopamine preparation: taking 100mg graphite oxide to be dispersed in 100mL deionized water, surpasses
Sonication forms uniform suspension；Take 100mg Dopamine hydrochloride to be dissolved in Tris-HCl(pH=8.5 of 150mL) buffering it is molten
It in liquid, is then added in graphite oxide suspension, is vigorously stirred at 60 DEG C ~ 65 DEG C for 24 hours, stand 30 ~ 40min, separation, water
It washes, it is dry to get redox graphene-poly-dopamine composite material rGO-PDA；
(3) redox graphene-poly-dopamine-glutathione preparation: take 30mg redox graphene-poly-dopamine molten
In Tris-HCl(pH=8.5 30mL) buffer solution in, be ultrasonically treated 5 ~ 6 hours；30mg glutathione is taken to be added to reduction
In graphene oxide-poly-dopamine solution, the manganese dioxide of a small amount of (6mg) is added, it is small to be stirred to react 24 at 40 DEG C ~ 50 DEG C
When；After the reaction was completed, it filters, is freeze-dried 8 ~ 10h, obtains black solid rGO-PDA-GSH；
(4) preparation of modified electrode rGO-PDA/GCE, rGO-PDA-GSH/GCE: 5mg rGO-PDA, rGO-PDA- are taken respectively
GSH is dispersed in respectively in 5mL deionized water, and 1 ~ 2h of ultrasound obtains equal phase dispersant liquid.8 μ L this points are taken with microsyringe respectively
The direct drop coating of dispersion liquid in the bare glassy carbon electrode surface that diameter is 3mm, (use on chamois leather respectively before being modified by bare glassy carbon electrode
0.3 μm and 0.05 μm of Al2O3Powder is polished to mirror surface, after washing surface dirt, is transferred in ultrasonic water bath successively with anhydrous second
Alcohol, nitric acid (1:1, V/V) and ultrapure water continuously wash 2min), finally, modified electrode is placed to 6min at room temperature makes electrode table
The solvent evaporating completely in face obtains electrode rGO-PDA/GCE, rGO-PDA-GSH/GCE of composite material modification, that is, work electricity
Pole；
(5) electric performance test: respectively using modified electrode rGO-PDA/GCE, rGO-PDA-GSH/GCE as working electrode, platinum electrode
For to electrode, saturated calomel electrode is reference electrode, electrochemical workstation is established；Modified electrode rGO-PDA-GSH/GCE is set
In 5.0mM [Fe (CN)6]4-/3-Be 50mV/s sweeping speed in KCl solution comprising 0.1M, under scanning range -0.2V ~ 0.6V into
Its electric conductivity of row cyclic voltammetry.The results show that electric current 104.21 the μ A, rGO-PDA of the composite material (rGO-PDA-GSH)
55.34 μ A of electric current.
Embodiment 2
(1) preparation of graphite oxide: with embodiment 1；
(2) 100mg graphite oxide redox graphene-poly-dopamine preparation: is dispersed in (concentration in 50mL deionized water
2mg/ml), ultrasonic treatment forms uniform suspension；Then 50mg Dopamine hydrochloride is dissolved in the Tris-Hcl(pH of 150mL
=8.5) it in buffer solution, then adds it in the suspension of graphite oxide, is vigorously stirred for 24 hours at 60 DEG C ~ 65 DEG C, it is quiet
30 ~ 40min is set, is separated, washing is dry to get redox graphene-poly-dopamine composite material；
(3) redox graphene-poly-dopamine-glutathione preparation: take 30mg redox graphene-poly-dopamine molten
In Tris-Hcl(pH=8.5 of 30mL) buffer solution in, be ultrasonically treated 5 ~ 6 hours；60mg glutathione is added to reduction
In graphene oxide-poly-dopamine solution, the manganese dioxide of a small amount of (13.5mg) is then added, it is anti-in 40 DEG C ~ 50 DEG C stirrings
It answers 24 hours；After the reaction was completed, it filters, is freeze-dried 8 ~ 10h, obtains black solid redox graphene-poly-dopamine-paddy
The sweet peptide rGO-PDA-GSH of Guang；
(4) preparation of modified electrode rGO-PDA/GCE, rGO-PDA-GSH/GCE: with embodiment 1；
(5) rGO-PDA-GSH electric performance test: test method is the same as embodiment 1.The results show that composite material rGO-PDA-GSH
The 79.04 μ A of electric current of electric current 142.12 μ A, rGO-PDA.
Claims (5)
1. a kind of preparation method of redox graphene-poly-dopamine-glutathione composite material, including following technique step
It is rapid:
(1) redox graphene-poly-dopamine preparation: graphite oxide ultrasonic disperse is formed in deionized water uniformly outstanding
Supernatant liquid；Dopamine hydrochloride is dissolved in the Tris-HCl buffer solution of pH=8.5, is then added in graphite oxide suspension, is mixed
The stirring 22 ~ for 24 hours at 60 DEG C ~ 65 DEG C, stands, separates, wash after closing uniformly, dry, obtains redox graphene-poly-dopamine
Composite material；
(2) redox graphene-poly-dopamine-glutathione composite material preparation: by the poly- DOPA of redox graphene-
Amine is dissolved in the Tris-HCl buffer solution of pH=8.5, is ultrasonically treated 5 ~ 6h；Glutathione, catalyst manganese dioxide are added,
Reaction 22 ~ for 24 hours at room temperature, is filtered after the reaction was completed, dry, obtains black solid redox graphene-poly-dopamine-paddy Guang
Sweet peptide.
2. a kind of preparation method of redox graphene-poly-dopamine-glutathione composite material as described in claim 1,
It is characterized by: the mass ratio of graphite oxide and poly-dopamine is 1:1 ~ 2:1 in step (1).
3. a kind of preparation method of redox graphene-poly-dopamine-glutathione composite material as described in claim 1,
It is characterized by: the mass ratio of redox graphene-poly-dopamine and glutathione is 1:1 ~ 1:2 in step (2).
4. a kind of preparation method of redox graphene-poly-dopamine-glutathione composite material as described in claim 1,
It is characterized by: the additional amount of catalyst manganese dioxide is redox graphene-poly-dopamine, glutathione in step (2)
Gross mass 10% ~ 15%.
5. a kind of preparation method of redox graphene-poly-dopamine-glutathione composite material as described in claim 1,
It is characterized by: the drying is dry 8 ~ 10h in freeze drying box in step (1), (2).
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111529682A (en) * | 2020-05-11 | 2020-08-14 | 中国人民解放军陆军军医大学第一附属医院 | Chemotaxis antibacterial nano material and preparation method and application thereof |
| CN112759807A (en) * | 2021-01-18 | 2021-05-07 | 中北大学 | High-thermal-conductivity three-dimensional graphene oxide composite functional particle modified natural rubber and preparation method thereof |
| CN113092558A (en) * | 2021-03-31 | 2021-07-09 | 中国药科大学 | Molecularly imprinted polymer electrode, preparation method and application thereof in electrochemical analysis of domoic acid |
| WO2021243825A1 (en) * | 2020-06-05 | 2021-12-09 | 青岛理工大学 | Hollow graphene oxide lubricating additive, super-lubricating water lubricant, preparation method therefor and use thereof |
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| NA HUANG ET AL.: "Multifunctional Electrochemical Platforms Based on the Michael Addition/Schiff Base Reaction of Polydopamine Modified Reduced Graphene Oxide: Construction and Application", 《ACS APPL. MATER. INTERFACES》 * |
| 黄娜: "几种功能化石墨烯纳米复合材料的制备及其电化学传感研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
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|---|---|---|---|---|
| CN111529682A (en) * | 2020-05-11 | 2020-08-14 | 中国人民解放军陆军军医大学第一附属医院 | Chemotaxis antibacterial nano material and preparation method and application thereof |
| WO2021243825A1 (en) * | 2020-06-05 | 2021-12-09 | 青岛理工大学 | Hollow graphene oxide lubricating additive, super-lubricating water lubricant, preparation method therefor and use thereof |
| CN112759807A (en) * | 2021-01-18 | 2021-05-07 | 中北大学 | High-thermal-conductivity three-dimensional graphene oxide composite functional particle modified natural rubber and preparation method thereof |
| CN112759807B (en) * | 2021-01-18 | 2022-05-24 | 中北大学 | High-thermal-conductivity three-dimensional graphene oxide composite functional particle modified natural rubber and preparation method thereof |
| CN113092558A (en) * | 2021-03-31 | 2021-07-09 | 中国药科大学 | Molecularly imprinted polymer electrode, preparation method and application thereof in electrochemical analysis of domoic acid |
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