CN109776651A - One kind is based on low immunogenicity polypeptide micro belt and preparation method thereof - Google Patents
One kind is based on low immunogenicity polypeptide micro belt and preparation method thereof Download PDFInfo
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- CN109776651A CN109776651A CN201910047714.0A CN201910047714A CN109776651A CN 109776651 A CN109776651 A CN 109776651A CN 201910047714 A CN201910047714 A CN 201910047714A CN 109776651 A CN109776651 A CN 109776651A
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- low immunogenicity
- polypeptide
- micro belt
- self
- assembling
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
Abstract
A kind of preparation method of the micro belt based on low immunogenicity self-assembling polypeptide, comprising the following steps: 1) first low immunogenicity polypeptide is at low temperature dissolved and is quickly stirred, it is sonicated to obtain polypeptide stoste；2) in sodium dihydrogen phosphate/disodium hydrogen phosphate buffer that pH is 9.0 ~ 13.0 by polypeptide compound concentration be then 0.01% ~ 30%(w/v) solution, at 20 DEG C ~ 37 DEG C, under light protected environment, in assembling for 24 hours ~ 48h in biochemical cultivation case；3) supercritical CO is then used2Solution is dried in dry or freeze-drying or air drying, finally obtains low immunogenicity polypeptide micro belt；Present invention process is simple and easy, obtained micro belt size uniformity, regular appearance, property are stablized, and there is good biocompatibility, biodegradable and biological safety, it can be used as drug carrier material, hemostatic material, tissue engineering bracket, advanced dressing, beauty treatment material etc..
Description
Technical field
The invention belongs to biomedical material technology, relate to it is a kind of based on low immunogenicity polypeptide micro belt and its
Preparation method.
Background technique
Micro belt has its special performance and purposes as a kind of micro materials of special construction.Since micro belt is by for the first time
It was found that the hereafter research of the micro belt of a variety of materials is always forward position and the hot spot of international field of new materials, and acquirement is made us looking steadily
Purpose achievement.Scientific circles personage prophesy, polypeptide micro belt is with its unique structure and excellent biology performance, especially in power
Learning aspect of performance has unique advantage, will there is tempting application prospect and huge potential using value in medicine.
Polypeptide has high-biocompatibility, and low immunogenicity, excellent bioactivity and suitable biological degradability etc. are all
More advantages.Self assembly refers to using a molecule or multiple molecules as basic unit, by non-covalent bond power drive, with from lower and
On construct mode, spontaneously form it is with high-sequential, stable and regular, and with certain microscopic appearance aggregation
Body.Study of Self-assembling Peptides is with its self assembly driving force abundant, special function and good biocompatibility, in biological material
Material, medicament transport, organizational project etc. are with a wide range of applications.
In conclusion the present invention should not regulate and control to solve biological micro materials appearance and size at present, poor biocompatibility,
A kind of based on low immunogenicity polypeptide micro belt, pattern spy of the micro belt with regular appearance, size uniformity has been prepared
Point, and polypeptide micron can be used as with excellent performances such as good biocompatibility, biodegradable and biological safeties
Drug carrier material, hemostatic material, tissue engineering bracket, advanced dressing etc..
Summary of the invention
The purpose of the present invention is in view of the deficiencies of the prior art and provide based on low immunogenicity polypeptide micro belt.This is micro-
Rice belt has regular appearance, the pattern feature of size uniformity, and polypeptide micron has good biocompatibility, biological can drop
The excellent performances such as solution property and biological safety, can be used as drug carrier material, hemostatic material, tissue engineering bracket, advanced dressing
Deng.
To achieve the above object, the present invention adopts the following technical scheme:
1) it is 0.01% ~ 30%(w/v by low immunogenicity polypeptide and disodium hydrogen phosphate/phosphate sodium dihydrogen buffer solution (PBS) ratio),
At pH 9.0~13.0, low temperature is sufficiently dissolved and is ultrasonically treated, until after as clear as crystal；
2) it by it at 20 DEG C ~ 37 DEG C, in dark surrounds, places in biochemical cultivation case and assembles for 24 hours ~ 48h；
3) solution is utilized into supercritical CO again2Dry or freeze-drying or air drying, sample after drying is examined with scanning electron microscope
Low immunogenicity self-assembling polypeptide micro belt can be obtained in survey.
This technology compared with prior art, has the advantages that
(1) different from conventional animal source collagen, the present invention ensure that material using low immunogenicity polypeptide as raw material from the root
The biological safety of material, meanwhile, low immunogenicity polypeptide equally has excellent bioactivity；
(2) low immunogenicity polypeptide micro belt prepared by the present invention is easy to operation, and the micro belt of preparation has preferable raw
Object compatibility, bioactivity and biological degradability are expected to be applied in fields such as pharmaceutical carrier, organizational project, advanced dressing.
(3) present invention uses the microscopic appearance for capableing of characteristic design low immunogenicity polypeptide with Study of Self-assembling Peptides, makes
Obtaining polypeptide micro belt has regular appearance, size uniformity.
Detailed description of the invention
Fig. 1 is low immunogenicity polypeptide micro belt of the invention.
Specific embodiment
Below by implementing that the present invention is specifically described, it is necessary to which indicated herein is that the present embodiment is served only for pair
The present invention is further described, and should not be understood as limiting the scope of the invention, the person skilled in the art in the field
Nonessential modifications and adaptations can be made according to the content of foregoing invention.
Embodiment 1
1) it is 0.01%(w/v by low immunogenicity polypeptide and PBS ratio), at pH 9.0, low temperature sufficiently dissolves and surpasses
Sonication, until after as clear as crystal；
2) it by it at 37 DEG C, under light protected environment, places and is assembled for 24 hours in biochemical cultivation case；
3) solution is utilized into supercritical CO again2It is dry, it is more that low immunogenicity is can be obtained into sample scanning electron microscope detection after drying
Self-assembling peptide micro belt.
In above-mentioned steps, the corresponding relationship of quality and volume is 1 mass parts: 1 volume=1g:1ml.
The mean breadth of this micro belt is at 1 μm.
Embodiment 2
1) it is 0.1%(w/v by low immunogenicity polypeptide and PBS ratio), at pH 10.0, low temperature sufficiently dissolves and surpasses
Sonication, until after as clear as crystal；
2) it by it at 37 DEG C, under light protected environment, places in biochemical cultivation case and assembles 36h；
3) again by solution, using freeze-drying, low immunogenicity polypeptide is can be obtained into certainly in sample scanning electron microscope detection after drying
Assemble micro belt.
In above-mentioned steps, the corresponding relationship of quality and volume is 1 mass parts: 1 volume=1g:1ml.
The mean breadth of this micro belt is at 2 μm.
Embodiment 3
1) it is 10%(w/v by low immunogenicity polypeptide and PBS ratio), at pH 12.0, low temperature sufficiently dissolves and surpasses
Sonication, until after as clear as crystal；
2) it by it at 37 DEG C, under light protected environment, places in biochemical cultivation case and assembles 48h；
3) again by solution, using air drying, low immunogenicity polypeptide is can be obtained into certainly in sample scanning electron microscope detection after drying
Assemble micro belt.
In above-mentioned steps, the corresponding relationship of quality and volume is 1 mass parts: 1 volume=1g:1ml
The mean breadth of this micro belt is at 0.5 μm.
Embodiment 4
1) it is 0.1%(w/v by low immunogenicity polypeptide and PBS ratio), at pH 10.0, low temperature sufficiently dissolves and surpasses
Sonication, until after as clear as crystal；
2) it by it at 20 DEG C, under light protected environment, places in biochemical cultivation case and assembles 36h；
3) again by solution, using freeze-drying, low immunogenicity polypeptide is can be obtained into certainly in sample scanning electron microscope detection after drying
Assemble micro belt.
In above-mentioned steps, the corresponding relationship of quality and volume is 1 mass parts: 1 volume=1g:1ml.
Claims (7)
1. a kind of micro belt based on low immunogenicity self-assembling polypeptide, which is characterized in that by low immunogenicity self-assembling polypeptide
Made of ribbon pattern, have nanoscale thickness and micron-sized width.
2. a kind of preparation method of the micro belt based on low immunogenicity self-assembling polypeptide, which comprises the following steps:
Low immunogenicity polypeptide is dissolved in using PBS buffer solution to adjust pH to 9.0~13.0, being protected from light in the system of decentralized medium
Under environment, 20 DEG C ~ 40 DEG C assembling for 24 hours ~ 48h obtain a kind of micro belt based on low immunogenicity self-assembling polypeptide.
3. a kind of preparation method of micro belt based on low immunogenicity self-assembling polypeptide according to claim 2, special
Sign is that the dosage of the low immunogenicity polypeptide is the every 100mlPBS buffer of 0.01 ~ 30g.
4. a kind of preparation method of micro belt based on low immunogenicity self-assembling polypeptide according to claim 2, special
Sign is, comprising the following steps:
1) low immunogenicity polypeptide and disodium hydrogen phosphate/phosphate sodium dihydrogen buffer solution are pressed into w/v=0.01 ~ 30g/100ml, in pH
Under 9.0~13.0, low temperature is sufficiently dissolved and is ultrasonically treated, until as clear as crystal；
2) it by step 1) product at 20 DEG C ~ 37 DEG C, under light protected environment, places in biochemical cultivation case and assembles for 24 hours ~ 48h；
3) by step 2 product, supercritical CO is utilized2Dry or freeze-drying or air drying, it is more to be prepared low immunogenicity
Self-assembling peptide micro belt.
5. a kind of preparation method of micro belt based on low immunogenicity self-assembling polypeptide according to claim 4, special
Sign is, comprising the following steps:
It 1) is 0.01g/100ml by low immunogenicity polypeptide and PBS ratio, at pH 9.0, low temperature sufficiently dissolves simultaneously
Ultrasonic treatment, until after as clear as crystal；
2) it by it at 37 DEG C, under light protected environment, places and is assembled for 24 hours in biochemical cultivation case；
3) solution is utilized into supercritical CO again2It is dry, low immunogenicity self-assembling polypeptide micro belt is prepared.
6. a kind of preparation method of micro belt based on low immunogenicity self-assembling polypeptide according to claim 4, special
Sign is, comprising the following steps:
It 1) is 0.1g/100ml by low immunogenicity polypeptide and PBS ratio, at pH 10.0, low temperature sufficiently dissolves simultaneously
Ultrasonic treatment, until as clear as crystal；
2) it by it at 37 DEG C, under light protected environment, places in biochemical cultivation case and assembles 36h；
3) low immunogenicity self-assembling polypeptide micro belt is prepared using freeze-drying in solution again.
7. a kind of preparation method of micro belt based on low immunogenicity self-assembling polypeptide according to claim 4, special
Sign is, comprising the following steps:
It 1) is 10g/100ml by low immunogenicity polypeptide and PBS ratio, at pH 12.0, low temperature sufficiently dissolves and surpasses
Sonication, until as clear as crystal；
2) it by it at 37 DEG C, in light protected environment, places in biochemical cultivation case and assembles 48h；
3) low immunogenicity self-assembling polypeptide micro belt is prepared using air drying in solution again.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910047714.0A CN109776651A (en) | 2019-01-18 | 2019-01-18 | One kind is based on low immunogenicity polypeptide micro belt and preparation method thereof |
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| CN201910047714.0A CN109776651A (en) | 2019-01-18 | 2019-01-18 | One kind is based on low immunogenicity polypeptide micro belt and preparation method thereof |
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| CN109776651A true CN109776651A (en) | 2019-05-21 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150306276A1 (en) * | 2014-04-24 | 2015-10-29 | Warsaw Orthopedic, Inc. | Collagen matrix |
| CN107630060A (en) * | 2016-07-19 | 2018-01-26 | 华南生物医药研究院 | Self assembly collagen and preparation method thereof |
| CN108939132A (en) * | 2018-07-18 | 2018-12-07 | 陕西科技大学 | A kind of high bioactivity hyaluronic acid medical film and preparation method thereof based on the modification of low immunogenicity collagen |
-
2019
- 2019-01-18 CN CN201910047714.0A patent/CN109776651A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150306276A1 (en) * | 2014-04-24 | 2015-10-29 | Warsaw Orthopedic, Inc. | Collagen matrix |
| CN107630060A (en) * | 2016-07-19 | 2018-01-26 | 华南生物医药研究院 | Self assembly collagen and preparation method thereof |
| CN108939132A (en) * | 2018-07-18 | 2018-12-07 | 陕西科技大学 | A kind of high bioactivity hyaluronic acid medical film and preparation method thereof based on the modification of low immunogenicity collagen |
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