CN109776220A - A kind of fertilizer improving tobacco drought-resistant ability - Google Patents
A kind of fertilizer improving tobacco drought-resistant ability Download PDFInfo
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- CN109776220A CN109776220A CN201910259623.3A CN201910259623A CN109776220A CN 109776220 A CN109776220 A CN 109776220A CN 201910259623 A CN201910259623 A CN 201910259623A CN 109776220 A CN109776220 A CN 109776220A
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Abstract
The invention discloses a kind of fertilizer for improving tobacco drought-resistant ability, belong to crop fertilizer field.The fertilizer comprises the following components in parts by weight: 20-30 parts of plant ash, 3-5 parts of ammonium nitrate, 1-3 parts of ammonium dihydrogen phosphate, 4-7 parts of potassium chloride, fluorescent pseudomonasPseudomonas fluorescens3-8 parts, bacillus subtilisBacillus subtilis3-8 parts.Tobacco is planted using fertilizer provided by the invention, the drought-resistant ability of tobacco can be significantly improved, and then improve the yield of tobacco.
Description
Technical field
The invention belongs to field of plant fertilizer, and in particular, to it is a kind of improve tobacco drought-resistant ability fertilizer and its preparation
Method.
Background technique
Tobacco (scientific name: Nicotiana tabacum L.) is Solanaceae Nicotiana plant, annual or limited perennial grass
This.Raw material of the tobacco as tobacco industry, complete stool can also make agricultural chemical insecticide；Also pharmaceutically acceptable, it anaesthetizes, sweating, calmness and urge
Vomitory.With important economic value.
Tobacco originates from the torrid zone of abundant rainfall, and the requirement to moisture is very high, and leaf water content reduces 6%~8%, that is, goes out
It now wilts phenomenon.The southwest that major part cigarette district especially tobacco field in China's is concentrated, Chang Yin lack necessary irrigating facility, cause
Moisture, which lacks, becomes the principal element for restricting tobacco planting and quality responses.Therefore, tobacco drought resistance is improved to be of great significance.
Summary of the invention
It is an object of the invention to provide a kind of raising tobacco drought resisting energy in view of the above-mentioned problems, overcome the shortage of prior art
The fertilizer of power.
To achieve the above object, the present invention provides a kind of fertilizer for improving tobacco drought-resistant ability comprising following parts by weight
Component: 20-30 parts of plant ash, 3-5 parts of ammonium nitrate, 1-3 parts of ammonium dihydrogen phosphate, 4-7 parts of potassium chloride, fluorescent pseudomonas 3-8
Part, 3-8 parts of bacillus subtilis.
In some embodiments of the present invention, the fluorescent pseudomonas is selected from P.fluorescens ATCC
13525, at least one of P.fluorescens ATCC 17563, P.fluorescens JCM 13057.
In some specific embodiments of the invention, the fluorescent pseudomonas is P.fluorescens ATCC
13525, the fluorescent pseudomonas mixing of P.fluorescens ATCC 17563, P.fluorescens JCM 13057 composition
Bacterium powder.
In one embodiment of the invention, fluorescent pseudomonas bacterium in the fluorescent pseudomonas Mixed Microbes powder
The content of body is 1 × 108~3 × 1010A/mg.
In another specific embodiment of the invention, the ratio of each bacterium in the fluorescent pseudomonas Mixed Microbes powder
Are as follows: P.fluorescens ATCC 13525:P.fluorescens ATCC 17563:P.fluorescens JCM 13057
=1:1.5:2.
In other embodiment of the invention, the bacillus subtilis be selected from B.subtilis DSM21393,
At least one of B.subtilis ATCC 31578 and B.subtilis NBRC 3009.
In some specific embodiments of the invention, the bacillus subtilis be B.subtilis DSM21393,
The bacillus subtilis Mixed Microbes powder that B.subtilis ATCC 31578 and B.subtilis NBRC 3009 is formed.
In one embodiment of the invention, bacillus subtilis bacterium in the bacillus subtilis Mixed Microbes powder
The content of body is 1 × 108~3 × 1010A/mg.
In another specific embodiment of the invention, the ratio of each bacterium in the bacillus subtilis Mixed Microbes powder
Are as follows: B.subtilis DSM 21393:B.subtilis ATCC 31578:B.subtilis NBRC 3009=1:0.5:
1.5。
In some specific embodiments of the invention, the fertilizer is comprised the following components in parts by weight: 22 parts of plant ash,
3.5 parts of ammonium nitrate, 1.7 parts of ammonium dihydrogen phosphate, 4.2 parts of potassium chloride, 5 parts of fluorescent pseudomonas, 6 parts of bacillus subtilis.
In other specific embodiments of the invention, the fertilizer is comprised the following components in parts by weight: plant ash 25
Part, 4.1 parts of ammonium nitrate, 2.2 parts of ammonium dihydrogen phosphate, 5 parts of potassium chloride, 4 parts of fluorescent pseudomonas, 7 parts of bacillus subtilis.
In other specific embodiment of the invention, the fertilizer is comprised the following components in parts by weight: plant ash 28
Part, 3.8 parts of ammonium nitrate, 2.7 parts of ammonium dihydrogen phosphate, 5.6 parts of potassium chloride, 5 parts of fluorescent pseudomonas, 5 parts of bacillus subtilis.
Beneficial effects of the present invention
Tobacco is planted using fertilizer provided by the invention, the drought-resistant ability of tobacco can be significantly improved, and then improve tobacco
Yield.Specifically, under simulated conditions, fertilizer provided by the invention can allow the tobacco simulating drought of resistance to 25%PEG-6000
Stress.Also, fertilizer of the invention can be improved 30% or more tobacco production.
Detailed description of the invention
Fig. 1 shows the influence of fluorescent pseudomonas and bacillus subtilis to the anti-Drought stress simulation of tobacco is improved,
CK1: deionized water processing；CK2:PEG-6000 processing；The processing of A:PEG-6000+ fluorescent pseudomonas；B:PEG-6000+ withered grass
Bacillus processing；C:PEG-6000+ fluorescent pseudomonas+bacillus subtilis processing.
Influence Fig. 2 shows fertilizer #1-#3 to tobacco drought-resistant ability, CK1: non-arid plantation；CK2: arid plantation；A:
Arid plantation+fertilizer #1；B: arid plantation+fertilizer #2；C: arid plantation+fertilizer #3.
Specific embodiment
In order to which the technical problems, technical solutions and beneficial effects solved by the present invention is more clearly understood, below in conjunction with
Embodiment, the present invention will be described in further detail.
Embodiment
Following example is used herein to demonstration the preferred embodiments of the invention.Those skilled in the art, it will be appreciated that under
State the technology disclosed in example represent inventor discovery can be used for implementing technology of the invention, therefore can be considered as implementation this
The preferred embodiment of invention.But those skilled in the art should be understood that specific reality disclosed herein according to this specification
Many modifications can be made by applying example, still can be obtained identical or similar as a result, rather than away from the spirit or scope of the present invention.
Unless otherwise defined, the term of all technologies as used herein and science, and the technology in fields of the present invention
Personnel institute is normally understood equivalent in meaning, and being disclosed reference and their materials of reference will all be incorporated.
Those skilled in the art will recognize or just will appreciate that by routine test many described here
Invention specific embodiment many equivalent technologies.These will equally be comprised in claims.
The fertilizer #1 of the raising tobacco drought-resistant ability of embodiment 1
The present embodiment provides a kind of fertilizer that can be improved tobacco drought-resistant ability, number #1 comprising following parts by weight
Component: it is 22 parts of plant ash, 3.5 parts of ammonium nitrate, 1.7 parts of ammonium dihydrogen phosphate, 4.2 parts of potassium chloride, 5 parts of fluorescent pseudomonas, withered
6 parts of careless bacillus.
Wherein, fluorescent pseudomonas be P.fluorescens ATCC 13525, P.fluorescens ATCC17563,
The fluorescent pseudomonas Mixed Microbes powder that P.fluorescens JCM 13057 is formed.Fluorescent pseudomonas thallus in Mixed Microbes powder
Content be 2 × 109A/mg, the ratio of each bacterium are as follows: P.fluorescens ATCC13525:P.fluorescens ATCC
17563:P.fluorescens JCM 13057=1:1.5:2.
Bacillus subtilis is B.subtilis DSM 21393, B.subtilis ATCC 31578 and B.subtilis
The bacillus subtilis Mixed Microbes powder that NBRC 3009 is formed.The content of bacillus subtilis thallus is 2 × 10 in Mixed Microbes powder9
A/mg, the ratio of each bacterium are as follows: B.subtilis DSM 21393:B.subtilis ATCC31578:B.subtilis NBRC
3009=1:0.5:1.5.
The fertilizer #2 of the raising tobacco drought-resistant ability of embodiment 2
The present embodiment provides a kind of fertilizer that can be improved tobacco drought-resistant ability, number #2 comprising following parts by weight
Component: it is 22 parts of plant ash, 3.5 parts of ammonium nitrate, 1.7 parts of ammonium dihydrogen phosphate, 4.2 parts of potassium chloride, 5 parts of fluorescent pseudomonas, withered
6 parts of careless bacillus.
Wherein, fluorescent pseudomonas be P.fluorescens ATCC 13525, P.fluorescens ATCC17563,
The fluorescent pseudomonas Mixed Microbes powder that P.fluorescens JCM 13057 is formed.Fluorescent pseudomonas thallus in Mixed Microbes powder
Content be 2 × 109A/mg, the ratio of each bacterium are as follows: P.fluorescens ATCC13525:P.fluorescens ATCC
17563:P.fluorescens JCM 13057=1:1.5:2.
Bacillus subtilis is B.subtilis DSM 21393, B.subtilis ATCC 31578 and B.subtilis
The bacillus subtilis Mixed Microbes powder that NBRC 3009 is formed.The content of bacillus subtilis thallus is 2 × 10 in Mixed Microbes powder9
A/mg, the ratio of each bacterium are as follows: B.subtilis DSM 21393:B.subtilis ATCC31578:B.subtilis NBRC
3009=1:0.5:1.5.
The fertilizer #3 of the raising tobacco drought-resistant ability of embodiment 3
The present embodiment provides a kind of fertilizer that can be improved tobacco drought-resistant ability, number #1 comprising following parts by weight
Component: it is 22 parts of plant ash, 3.5 parts of ammonium nitrate, 1.7 parts of ammonium dihydrogen phosphate, 4.2 parts of potassium chloride, 5 parts of fluorescent pseudomonas, withered
6 parts of careless bacillus.
Wherein, fluorescent pseudomonas be P.fluorescens ATCC 13525, P.fluorescens ATCC17563,
The fluorescent pseudomonas Mixed Microbes powder that P.fluorescens JCM 13057 is formed.Fluorescent pseudomonas thallus in Mixed Microbes powder
Content be 2 × 109A/mg, the ratio of each bacterium are as follows: P.fluorescens ATCC13525:P.fluorescens ATCC
17563:P.fluorescens JCM 13057=1:1.5:2.
Bacillus subtilis is B.subtilis DSM 21393, B.subtilis ATCC 31578 and B.subtilis
The bacillus subtilis Mixed Microbes powder that NBRC 3009 is formed.The content of bacillus subtilis thallus is 2 × 10 in Mixed Microbes powder9
A/mg, the ratio of each bacterium are as follows: B.subtilis DSM 21393:B.subtilis ATCC31578:B.subtilis NBRC
3009=1:0.5:1.5.
The influence of 4 fluorescent pseudomonas of embodiment and bacillus subtilis to the anti-Drought stress simulation of tobacco is improved
Fluorescent pseudomonas bacterium solution: thallus content is 1 × 10 after above-mentioned fluorescent pseudomonas Mixed Microbes powder is pressed dissolution9
A/mL is dissolved in sterile water.
Bacillus subtilis bacterium solution: thallus content is 1 × 10 after above-mentioned bacillus subtilis Mixed Microbes powder is pressed dissolution9
A/mL is dissolved in sterile water.
Tobacco seed 5min is sterilized with 0.5% liquor natrii hypochloritis, is subsequently placed in (diameter 9cm) training for being covered with 2 layers of filter paper
It supports and germinates in ware.4mL deionized water is added in control group 1 (CK1)；Control group 2 (CK2) is added after 2mL 25%PEG-6000 to be added again
Enter 2mL deionized water；Experimental group is divided into 3 groups, and after each addition 2mL 25%PEG-6000, A group adds 2mL fluorescent pseudomonas
Bacterium solution, B group add 2mL bacillus subtilis bacterium solution；C group is separately added into 1mL fluorescent pseudomonas bacterium solution and 1mL withered grass bud again
Spore bacillus bacterium solution.
Seed uses Parafilm sealed membrane to seal culture dish after setting bed.20 DEG C (12h) of dark, illumination 30 DEG C of (12h, illumination
Intensity 4000lx) alternating temperature germination.Test is repeated 3 times, 100 seeds of every repetition.16th day calculating germination percentage.
As a result as shown in table 1 and Fig. 1, it is known that, after PEG-6000 stress, the germination percentage of seed can be decreased significantly, and sharp
Tobacco seed can be significantly improved to the patience of drought stress with fluorescent pseudomonas and/or bacillus subtilis, wherein withered grass bud
Spore bacillus effect is better than fluorescent pseudomonas, and to share effect best for the two.
The influence of 1 fluorescent pseudomonas of table and bacillus subtilis to the anti-Drought stress simulation of tobacco is improved
Influence of the 5 fertilizer #1-#3 of embodiment to tobacco drought-resistant ability
It is administered in Tobacco Farm respectively using the fertilizer #1-#3 that embodiment 1-3 is provided, as a result, it has been found that (table 2, Fig. 2), arid
Under the conditions of, relative to non-arid plantation, tobacco production is remarkably decreased, 35% or more is reduced, and it is equal to apply three kinds of different fertilizer
It can restore even to improve arid planting conditions tobacco content.
| Number | Processing | Output increased % |
| CK1 | Non- arid plantation | —— |
| CK2 | Arid plantation | -35.24±3.16 |
| A | Arid plantation+fertilizer #1 | 22.34±3.78 |
| B | Arid plantation+fertilizer #2 | 15.28±2.17 |
| C | Arid plantation+fertilizer #3 | 14.29±3.21 |
All references mentioned in the present invention is incorporated herein by reference, independent just as each document
It is incorporated as with reference to such.In addition, it should also be understood that, after reading the above teachings of the present invention, those skilled in the art can
To make various changes or modifications to the present invention, such equivalent forms equally fall within model defined by the application the appended claims
It encloses.
Claims (10)
1. a kind of fertilizer for improving tobacco drought-resistant ability, which is characterized in that comprise the following components in parts by weight: plant ash 20-30
Part, 3-5 parts of ammonium nitrate, 1-3 parts of ammonium dihydrogen phosphate, 4-7 parts of potassium chloride, 3-8 parts of fluorescent pseudomonas, bacillus subtilis 3-8
Part.
2. fertilizer according to claim 1, which is characterized in that the fluorescent pseudomonas is selected from P.fluorescens
At least one of ATCC 13525, P.fluorescens ATCC 17563, P.fluorescens JCM 13057.
3. fertilizer according to claim 2, which is characterized in that the fluorescent pseudomonas is P.fluorescens ATCC
13525, the fluorescent pseudomonas mixing of P.fluorescens ATCC 17563, P.fluorescens JCM 13057 composition
Bacterium powder.
4. fertilizer according to claim 3, which is characterized in that the ratio of each bacterium in the fluorescent pseudomonas Mixed Microbes powder
Example are as follows: P.fluorescens ATCC 13525:P.fluorescens ATCC 17563:P.fluorescens JCM
13057=1:1.5:2.
5. fertilizer according to claim 1, which is characterized in that the bacillus subtilis is selected from B.subtilis DSM
21393, at least one of B.subtilis ATCC 31578 and B.subtilis NBRC 3009.
6. fertilizer according to claim 2, which is characterized in that the bacillus subtilis is B.subtilis DSM
21393, the bacillus subtilis Mixed Microbes powder that B.subtilis ATCC 31578 and B.subtilis NBRC 3009 is formed.
7. fertilizer according to claim 3, which is characterized in that the ratio of each bacterium in the bacillus subtilis Mixed Microbes powder
Example are as follows: B.subtilis DSM 21393:B.subtilis ATCC 31578:B.subtilis NBRC 3009=1:0.5:
1.5。
8. -7 any fertilizer according to claim 1, which is characterized in that comprise the following components in parts by weight: plant ash 22
Part, 3.5 parts of ammonium nitrate, 1.7 parts of ammonium dihydrogen phosphate, 4.2 parts of potassium chloride, 5 parts of fluorescent pseudomonas, 6 parts of bacillus subtilis.
9. -7 any fertilizer according to claim 1, which is characterized in that comprise the following components in parts by weight: plant ash 25
Part, 4.1 parts of ammonium nitrate, 2.2 parts of ammonium dihydrogen phosphate, 5 parts of potassium chloride, 4 parts of fluorescent pseudomonas, 7 parts of bacillus subtilis.
10. -7 any fertilizer according to claim 1, which is characterized in that comprise the following components in parts by weight: plant ash 28
Part, 3.8 parts of ammonium nitrate, 2.7 parts of ammonium dihydrogen phosphate, 5.6 parts of potassium chloride, 5 parts of fluorescent pseudomonas, 5 parts of bacillus subtilis.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910259623.3A CN109776220A (en) | 2019-04-02 | 2019-04-02 | A kind of fertilizer improving tobacco drought-resistant ability |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910259623.3A CN109776220A (en) | 2019-04-02 | 2019-04-02 | A kind of fertilizer improving tobacco drought-resistant ability |
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| Publication Number | Publication Date |
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| CN109776220A true CN109776220A (en) | 2019-05-21 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1416707A (en) * | 2002-12-12 | 2003-05-14 | 中国科学院新疆生态与地理研究所 | Microbial prepn for promoting germination of ephedra seed |
| CN102972447A (en) * | 2012-12-07 | 2013-03-20 | 江苏里下河地区农业科学研究所 | Biological seed-dressing agent |
| US20170027178A1 (en) * | 2015-07-28 | 2017-02-02 | Gregory R.H. Smith | Compositions and methods for improving plant health |
| CN108623390A (en) * | 2018-06-29 | 2018-10-09 | 贵州大学 | One grows tobacco special bio bacterial manure and the preparation method and application thereof |
-
2019
- 2019-04-02 CN CN201910259623.3A patent/CN109776220A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1416707A (en) * | 2002-12-12 | 2003-05-14 | 中国科学院新疆生态与地理研究所 | Microbial prepn for promoting germination of ephedra seed |
| CN102972447A (en) * | 2012-12-07 | 2013-03-20 | 江苏里下河地区农业科学研究所 | Biological seed-dressing agent |
| US20170027178A1 (en) * | 2015-07-28 | 2017-02-02 | Gregory R.H. Smith | Compositions and methods for improving plant health |
| CN108623390A (en) * | 2018-06-29 | 2018-10-09 | 贵州大学 | One grows tobacco special bio bacterial manure and the preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 刘炳花 等: "在水分胁迫下根际促生菌A2 对小麦萌芽期和苗期的促生效应", 《中国农学通报》 * |
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