CN109771698A - A kind of bone bracket complex and preparation method thereof - Google Patents
A kind of bone bracket complex and preparation method thereof Download PDFInfo
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- CN109771698A CN109771698A CN201910228394.9A CN201910228394A CN109771698A CN 109771698 A CN109771698 A CN 109771698A CN 201910228394 A CN201910228394 A CN 201910228394A CN 109771698 A CN109771698 A CN 109771698A
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Abstract
The embodiment of the invention discloses a kind of bone bracket complex and preparation method thereof, which includes the following steps: that calcium polyphosphate is ground up, sieved to obtain calcium polyphosphate amorphous powder by (a)；(b) obtained calcium polyphosphate amorphous powder is sintered, is to slowly warm up to 580 DEG C of holding 100-140min, then cooling obtains calcium polyphosphate bone holder material；(c) calcium polyphosphate bone holder material is mixed with icariin, then formed through 3D printing speed to get the bone bracket complex.The present invention obtains bone bracket complex by the way that icariin and calcium polyphosphate bone holder material to be mixed with, it can be realized slow releasing function of the bone bracket complex to icariin and calcium polyphosphate of preparation, it can promote the Osteoblast Differentiation of BMSCs by the collective effect of calcium polyphosphate and icariin, to repairing bone defect area, and repair fast, excellent effect.
Description
Technical field
The present embodiments relate to tissue engineering technique fields, and in particular to a kind of bone bracket complex and its preparation side
Method.
Background technique
Bone defect is that the structural intergrity of phalanges is destroyed, and is clinical common disease；Infection, tumour, wound, osteomyelitis
Operation wound clearing and various congenital disorders are the main reason for leading to bone defect；Clinically disease incidence is higher for bone defect, lacks
The bone at damage position is imperfect to make its shape and function defect occur, affects the quality of life of patient to some extent.
In orthopaedics, the reasons such as bone tumour, wound, osteomyelitis, osteonecrosis can cause different degrees of bone defect, especially
Large segmental bone defect, is a great problem that orthopaedics faces, and traditional bone graft material is mainly self allograph bone and man-made support,
Because body needs gradually to absorb bone graft material, reconstructs, effect is slower, and treatment cycle is long, and complication compares
It is more, in fact it could happen that bone defect again, patient suffering is more, seriously affects bone collection effect, and bone defect healing effect is poor.
Summary of the invention
For this purpose, the embodiment of the present invention provides a kind of preparation method of bone bracket complex, what which was prepared
Bone bracket complex can promote mesenchymal stem cell Osteoblast Differentiation, improve the repairing effect of bone defect to solve existing skill
In art using bone graft material existing effect is slow, complication is more, bone defect healing is poor problem.
To achieve the goals above, the embodiment of the present invention provides the following technical solutions:
A kind of preparation method of bone bracket complex is provided according to a first aspect of the embodiments of the present invention, the preparation method packet
Include following steps:
(a) calcium polyphosphate is ground up, sieved to obtain calcium polyphosphate amorphous powder；
(b) obtained calcium polyphosphate amorphous powder is sintered, is to slowly warm up to 580-585 DEG C of holding 100-
140min, then cooling obtains calcium polyphosphate bone holder material；
(c) calcium polyphosphate bone holder material is mixed with icariin, then formed through 3D printing speed to get the bone branch
Frame complex.
By being ground to calcium polyphosphate in the above-mentioned preparation method of the present invention, the bone branch being prepared can be improved
The intensity of frame complex, and the slow releasing function to calcium polyphosphate；Calcium polyphosphate can be improved by sintering, heating treatment again
Biomechanical property；In addition, the present invention is by being mixed with icariin and calcium polyphosphate bone holder material to obtain bone bracket
Complex can be realized the bone bracket complex of preparation to the slow releasing function of icariin and calcium polyphosphate, pass through calcium polyphosphate
It can promote the Osteoblast Differentiation of mesenchymal stem cell (BMSCs) with the collective effect of icariin, thus repairing bone defect
Area, and repair fast, excellent effect.
Further, parameter is arranged in the 3D printer that the 3D printing speed uses are as follows: and 400-600 μm of spray head, mobile speed
80-120mm/min is spent, bracket is stored as STL format.
Further, the mass ratio of the calcium polyphosphate bone holder material and icariin is (3-5): 1.By to poly- phosphorus
The restriction of sour calcium bone holder material and icariin dosage can be realized the two preferably synergistic effect, reach better promotion
The Osteoblast Differentiation of BMSCs improves the repairing effect of bone defect.
Further, the calcium polyphosphate amorphous powder partial size is 70-100 μm.By to calcium polyphosphate amorphous powder grain
The restriction of diameter can preferably promote the intensity for the bone bracket complex being prepared and the slow releasing function to calcium polyphosphate.
Further, the sintering temperature is 500-520 DEG C, time 110-120min.
Further, the slow heating rate is 2-3 DEG C/min.
The present invention can preferably improve the biomechanical property of calcium polyphosphate by the restriction of above-mentioned parameter.
The source of calcium polyphosphate is not limited strictly in the present invention, for example, can be commercially available by market；It is preferred that
Ground, the calcium polyphosphate are prepared via a method which to obtain:
By Ca (H2PO4)2·H2Calcium polyphosphate amorphous imitation frosted glass is made after O is kept the temperature, then after being warming up to melting, quenching；Again
Obtained calcium polyphosphate amorphous imitation frosted glass is cleaned, the calcium polyphosphate obtained by drying.
Further, the temperature of the heat preservation is 400-600 DEG C, time 50-70min.
Further, any one of the quenching media in air, inert gas and water.
Further, the solvent used that cleans is ethyl alcohol.
A kind of bone bracket complex is provided according to a second aspect of the embodiments of the present invention, and the bone bracket complex is by above-mentioned
Preparation method is prepared.
Bone bracket complex of the present invention has excellent mechanical strength, and makees to the sustained release of calcium polyphosphate and icariin
With.
The embodiment of the present invention has the advantages that
(1) by being ground to calcium polyphosphate in the above-mentioned preparation method of the present invention, it can be improved and be prepared
The intensity of bone bracket complex, and the slow releasing function to calcium polyphosphate；Polyphosphoric acid can be improved by sintering, heating treatment again
The biomechanical property of calcium.
It is (2) of the invention by being mixed with icariin and calcium polyphosphate bone holder material to obtain bone bracket complex,
The bone bracket complex of preparation be can be realized to the slow releasing function of icariin and calcium polyphosphate, pass through calcium polyphosphate and Herba Epimedii
The collective effect of glycosides can induce the Osteoblast Differentiation of BMSCs, thus repairing bone defect area, and repair fast, excellent effect.
(3) bone bracket complex of the present invention has excellent mechanical strength, and delays to calcium polyphosphate and icariin
Release effect.
Detailed description of the invention
It, below will be to embodiment party in order to illustrate more clearly of embodiments of the present invention or technical solution in the prior art
Formula or attached drawing needed to be used in the description of the prior art are briefly described.It should be evident that the accompanying drawings in the following description is only
It is merely exemplary, it for those of ordinary skill in the art, without creative efforts, can also basis
The attached drawing of offer, which is extended, obtains other implementation attached drawings.
Fig. 1 is the electron-microscope scanning figure for the bone bracket complex that the embodiment of the present invention 3 is prepared；
Fig. 2 is the coloration result figure of the BMSCs positive expression CD44 provided in experimental example of the present invention；
Fig. 3 is that the BMSCs feminine gender provided in experimental example of the present invention expresses the coloration result figure of CD34；
Fig. 4 is the x-ray inspection result of two groups of White Rabbits sample at 4 weeks, 8 weeks, 12 weeks condyles of femur in experimental example of the present invention
Figure；
Fig. 5 is that two groups of White Rabbits were dyed in 4 weeks, 8 weeks, the Van-Gieson of 12 weeks femur condyle tissues in experimental example of the present invention
Figure；
Fig. 6 is that using two groups of White Rabbits of micro- sem observation, at 4 weeks, 8 weeks, 12 weeks, BMSCs was migrated in experimental example of the present invention
Figure；
Fig. 7 is that two groups of White Rabbits of micro- sem observation nerve growth factor at 4 weeks, 8 weeks, 12 weeks is used in experimental example of the present invention
Sublist reaches level view.
Specific embodiment
Embodiments of the present invention are illustrated by particular specific embodiment below, those skilled in the art can be by this explanation
Content disclosed by book is understood other advantages and efficacy of the present invention easily, it is clear that described embodiment is the present invention one
Section Example, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not doing
Every other embodiment obtained under the premise of creative work out, shall fall within the protection scope of the present invention.
The raw material that following embodiment uses is as follows:
Icariin: purity 98%；Source Xi'an sky pavilion biology Co., Ltd, lot number YYH120318.
Embodiment 1
The present embodiment is a kind of preparation method of bone bracket complex, which includes the following steps:
(a) by Ca (H2PO4)2·H2O is warming up to melting after 400 DEG C of heat preservation 70min, carries out quenching using water and is made poly-
Calcium phosphate amorphous imitation frosted glass；Obtained calcium polyphosphate amorphous imitation frosted glass is cleaned using ethyl alcohol again, calcium polyphosphate obtained by drying；
(b) calcium polyphosphate is ground up, sieved to obtain the calcium polyphosphate amorphous powder that partial size is 70-100 μm；
(c) obtained calcium polyphosphate amorphous powder is sintered, 580 DEG C of holdings is then warming up to 2 DEG C/min
140min, then cooling obtains calcium polyphosphate bone holder material, and wherein sintering temperature is 500, time 120min；
(d) be according to mass ratio 5: 1 calcium polyphosphate bone holder material is mixed with icariin, then through 3D printing speed at
For type to get bone bracket complex, parameter is arranged in the 3D printer that wherein 3D printing speed uses are as follows: and 400 μm of spray head, movement speed
120mm/min, bracket are stored as STL format；Print parameters are that X-axis is 4mm, Y-axis 4mm, Z axis 10mm.
Embodiment 2
The present embodiment is a kind of preparation method of bone bracket complex, which includes the following steps:
(a) by Ca (H2PO4)2·H2O is warming up to melting after 600 DEG C of heat preservation 50min, carries out quenching using water and is made poly-
Calcium phosphate amorphous imitation frosted glass；Obtained calcium polyphosphate amorphous imitation frosted glass is cleaned using ethyl alcohol again, calcium polyphosphate obtained by drying；
(b) calcium polyphosphate is ground up, sieved to obtain the calcium polyphosphate amorphous powder that partial size is 70-100 μm；
(c) obtained calcium polyphosphate amorphous powder is sintered, 585 DEG C of holdings is then warming up to 3 DEG C/min
100min, then cooling obtains calcium polyphosphate bone holder material, and wherein sintering temperature is 585 DEG C, time 110min；
(d) be according to mass ratio 3: 1 calcium polyphosphate bone holder material is mixed with icariin, then through 3D printing speed at
For type to get bone bracket complex, parameter is arranged in the 3D printer that wherein 3D printing speed uses are as follows: and 600 μm of spray head, movement speed
80mm/min, bracket are stored as STL format；Print parameters are that X-axis is 4mm, Y-axis 4mm, Z axis 10mm.
Embodiment 3
The present embodiment is a kind of preparation method of bone bracket complex, which includes the following steps:
(a) by Ca (H2PO4)2·H2O is warming up to melting after 500 DEG C of heat preservation 60min, carries out quenching using water and is made poly-
Calcium phosphate amorphous imitation frosted glass；Obtained calcium polyphosphate amorphous imitation frosted glass is cleaned using ethyl alcohol again, calcium polyphosphate obtained by drying；
(b) calcium polyphosphate is ground up, sieved to obtain the calcium polyphosphate amorphous powder that partial size is 70-100 μm；
(c) obtained calcium polyphosphate amorphous powder is sintered, 582 DEG C of holdings is then warming up to 2.5 DEG C/min
120min, then cooling obtains calcium polyphosphate bone holder material, and wherein sintering temperature is 582, time 115min；
(d) be according to mass ratio 4: 1 calcium polyphosphate bone holder material is mixed with icariin, then through 3D printing speed at
For type to get bone bracket complex, parameter is arranged in the 3D printer that wherein 3D printing speed uses are as follows: and 500 μm of spray head, movement speed
100mm/min, bracket are stored as STL format；Print parameters are that X-axis is 4mm, Y-axis 4mm, Z axis 10mm.
The bone bracket complex that the above method is prepared is in the competing lower carry out Ultrastructural observation of electricity, and observation result is as schemed
Shown in 1, as shown in Figure 1, icariin is evenly distributed on the surface of bone bracket complex under Electronic Speculum.
By the above-mentioned bone bracket complex application finite element analysis software ANSYS 10.0 being prepared, carries out grid and draw
Divide, material properties assignment generates finite element model；Conditions setting is that Composite Bone bracket both ends are fixed；It is axially applied to bone branch
On frame, gradually apply 25N, 150N, 250N, 350N axial compressive force observes the ess-strain situation of different loads situation drag；
The results are shown in Table 1:
Table 1 is in 25N, 150N, 250N, the mean stress and maximum displacement table of 350N axial compressive force
| Pressure | Stress | It is displaced (in) | It is displaced (mm) |
| 25N | 2.632e+01 | 1.512e-02 | 0.43 |
| 150N | 1.467e+01 | 1.104e-01 | 2.48 |
| 250N | 2.727e+01 | 1.741e-01 | 4.46 |
| 350N | 3.228e+02 | 2.496e-01 | 6.21 |
As shown in Table 1: the bone bracket complex that the present invention is prepared has excellent mechanical property.
Experimental example
1 materials and methods
Experimental animal: health level, 3 monthly ages, weight 2.3-2.8kg, half male and half female, new zealand white rabbit 40,36 use
In establishing bone defect model, 4 are served only for preparation BMSCs, and new zealand white rabbit is provided by University Of Nanhua's animal experimental center, test
Animal and facility (facility address: the ever victorious road in hengyang, hunan province city 28) credit number: SYXK (Hunan) 2015-0001.
Main agents: bone bracket complex is prepared by embodiment 3；DMEM culture medium, fetal calf serum (U.S. Sigma
Company), immunohistochemistry mouse anti-rabbit CD34 polyclonal antibody, mouse anti-rabbit CD44 polyclonal antibody, rabbit-anti mouse nerve growth factor are more
Clonal antibody, goat anti-rabbit igg polyclonal antibody (Santa Cruz company, the U.S.), immunohistochemical kit, DAB colour reagent
Box, q-RTPCR kit (Invitrogen company, the U.S.).
2 experimental methods
The separation and culture of rabbit BMSCs: after new zealand white rabbit is anaesthetized successfully, sterile drape, from condyle of femur superior thyroid tubercle
Locate row bone marrow aspiration, marrow puncture needle has fall through sense after, extract puncture needle needle core out, connect syringe, extract 2ml bone marrow fluid, and
Opposite side bone marrow fluid is extracted with same method, the marrow of extraction is added on Percoll liquid level, 20min is centrifuged with 2000r/min,
It draws mononuclear cell layer (cloud), collects cell after PBS washing, DMEM culture medium (100U/ml containing penicillin, strepto- is added
Plain 100U/ml, 10% fetal calf serum of volume fraction) in culture, 48h full dose changes liquid, and every 3d carries out full dose and changes liquid later, for cell
Secondary culture is carried out when being paved with bottom of bottle substantially, is chosen the 3rd generation cell of passage and is tested.
Rabbit BMSCs identification: rabbit BMSCs is identified using Immunohistochemical Method: well-grown 3rd generation BMSCs being inoculated into and is put
Have and cultivated in the 6 orifice plates of coverslip for 24 hours, take out coverslip, makes to have one layer of cell upwards, normal saline flushing 2 times, dry,
Paraformaldehyde is fixed, and hydrogen peroxide is added and is incubated for 10min, distills water washing, mouse anti-rabbit CD34 antibody and mouse anti-rabbit CD44 is added
Antisera overnight is incubated for, and PBS liquid rinses, and DAB developing solution is added and is incubated for 10min, and PBS is washed, neutral gum mounting, is clapped under microscope
According to observation staining conditions, coloration result is as shown in Figure 2 and Figure 3；
By Fig. 2, Fig. 3 it is found that culture cell positive expresses CD44, feminine gender expression CD34, show that cultivating cell is BMSCs.
It establishes rabbit bone defect model and implantation bone bracket complex: making bone defect model at White Rabbit condyle of femur: will
After two groups of new zealand white rabbits are using the anesthesia of yellow Jackets auricular vein, dorsal position, field of operation preserved skin, disinfection, in great Bai are taken
Inner incision after rabbit bilateral distal femur, exposure White Rabbit condylus medialis femoris, takes and is used as boring point on entocondyle at 1.5cm, uses
Drill bit (diameter 4mm) drills through depth 10mm or so, avoids drilling through in boring procedure, and normal saline flushing bone bits are implanted into bone bracket
Complex is sewed up the incision, postoperative penicillin prevention infection 3d.
Grouping and processing: by be implanted into bone bracket complex after 36 White Rabbits according to random number method be divided into control group and
Experimental group, every group 18；While being implanted into bone bracket complex, experimental group White Rabbit is separately cultured through femoral vein injection
BMSCs 1.5ml(1×109A cell/L), control group White Rabbit is through being injected intravenously same amount of normal saline；Postoperative 4 weeks, 8 weeks, 12
All every dead 6 of component other places；The row bone marrow aspiration bone marrow extraction at condyle of femur superior thyroid tubercle according to the above method, separation and culture
BMSCs is measured for the measurement of BMSCs transport number and BMSCs Type I collagen albumen, CD44 level；Then it takes at rabbit bilateral condyle of femur
Sample, sample is measured for imageological examination and poroma Nerve Growth Factor Level at the condyle of femur of side, is marked at the condyle of femur of side
This is dyed for Van-Gieson.
3 testing results
3.1 imageological examinations: taking the photograph X-ray film for sample at postoperative 4 weeks of above-mentioned taking-up, 8 weeks, 12 weeks condyles of femur respectively, inspection
The fruit that comes to an end is as shown in Figure 4；
As shown in Figure 4, the 4th week, control group Composite Bone bracket and both ends femur interface were clear, no poroma wrapping, experimental group
Composite Bone bracket and both ends femur interface are fuzzy, and bone bracket side has a small amount of growth of spur；8th week, Composite Bone bracket and both ends
Femur interface is fuzzy, and bone bracket side has a small amount of poroma to generate, and observation group's Composite Bone bracket and both ends femur interface are fuzzy, bone branch
Frame side has more growth of spur；12nd week, control group Composite Bone bracket and both ends femur interface had a large amount of poroma to wrap up, real
Group Composite Bone branch frame peripheral is tested completely by newborn poroma tissue encapsulation.
3.2 histological observations: postoperative 4 weeks, 8 weeks, 12 weeks condyles of femur taken out are immersed in neutral formalin, taken after 7d
Out, without decalcification, gradient alcohol dehydration is placed in embedding liquid and embeds, cut by hard tissue slicing machine along femur long axis after 3 weeks
It is sliced in 4.5 μ m-thicks, observation histological change is dyed using Van-Gieson: using Weigert haematoxylin after paraffin section de-waxing
20min is dyed, blue 5min is returned, dyes 1min with Van-Gieson, abandons Van-Gieson dyeing liquor, alcohol breaks up the several seconds, dehydration,
Condyle of femur histological change is observed in transparent, sealing under lower 100 times of optical microscopy；It is as shown in Figure 5 to observe result；
As shown in Figure 5: the 4th week, experimental group bone bracket and area of new bone combination were closer, the degradation of bone stent edge portion, right
Obvious according to group bone bracket and area of new bone boundary, bone bracket is without obvious degradation；8th week, experimental group bone bracket and area of new bone boundary quilt
The wrapping of newborn bone trabecula, bone bracket are further degraded, and newborn bone trabecula is along the gap that bone scaffold degradation is formed to bone internal stent
Growth, the degradation of control group bone stent edge portion have a small amount of newborn bone trabecula；12nd week, experimental group bone bracket center degradation shape
At gap, newborn bone trabecula continues to grow into along bone scaffold degradation gap, and control group new life bone trabecula is few, and bone scaffold degradation is slow,
The range in the long bracket gap to the marrow of bone trabecula compares limitation.
3.3BMSCs transport number measurement: by postoperative 4 weeks of extraction, 8 weeks, the separation training again according to the method described above of 12 weeks marrow
It supports BMSCs and BMSCs transfer ability is measured using the cell Transwell: 2nd generation is added in the upper chamber of the 6 hole cells Transwell
BMSCs suspension (2 × 105A cell), it is placed in incubator and cultivates for 24 hours, take out the cell Transwell, discard culture solution, methanol
It is fixed, crystal violet method is dyed, and cotton swab wipes non-migrating cell, and observation invasion BMSCs is thin under inverted phase contrast microscope
Born of the same parents' number, for observation result as shown in fig. 6, BMSCs cell number statistical result is as shown in table 2, statistical method is soft using SPSS20.0
Part analysis, measurement data indicates with mean ± standard deviation, between control group and observation group each Indexes Comparison using t inspection, take P <
0.05 is statistically significant for difference；
2 two groups of White Rabbit BMSCs migrating cell numbers of table compare (n=6, ± s)
Note: * is compared with the control group P < 0.05.
By Fig. 6 and table 2 it is found that when the 4th week, 8 weeks, 12 weeks, experimental group White Rabbit BMSCs migrating cell number is above control
Group (P < 0.05).
3.4 BMSCs Type I collagen albumen, the measurement of CD44 level: postoperative 4 weeks, 8 weeks, 12 weeks 2nd generations being separately cultured are taken
BMSCs；BMSCs total serum IgE is extracted with Trizol method, total serum IgE reverse transcription is in cDNA, measures I type glue of BMSCs using q-RTPCR method
Former albumen, CD44mRNA are horizontal, using β-actin as internal reference；Type I collagen albumen, CD44mRNA expression quantity are with 2- Δ Δ CT table
Show；It is as shown in table 3 to measure statistical result；Statistical method is analyzed using SPSS20.0 software, and measurement data is with mean ± standard
Difference indicates that each Indexes Comparison is examined using t between control group and observation group, and taking P < 0.05 is that difference is statistically significant；
Table 3 two groups of White Rabbit BMSCs Type I collagen albumen, CD44 levels compare (n=6, ± s)
Note: * is compared with the control group P < 0.05.
As shown in Table 3, when the 4th week, 8 weeks, 12 weeks, experimental group White Rabbit BMSCs Type I collagen albumen, CD44mRNA are horizontal
It is above control group (P < 0.05).
The measurement of 3.5 poroma Nerve Growth Factor Levels: postoperative 4 weeks, 8 weeks, 12 weeks White Rabbit poroma are taken, paraformaldehyde is solid
Fixed, paraffin section measures poroma Nerve Growth Factor Level using Immunohistochemical Method: paraffin section is carried out immunohistochemistry dye
Color, with rabbit-anti mouse nerve growth factor (1:100) for primary antibody, with goat anti-rabbit igg (1:1000) for secondary antibody, DAB colour developing, microscope
Lower observation expression of nerve growth situation, observation result are inhaled as shown in fig. 7, being analyzed using Image-pro Plus6.0 software
Luminosity, Nerve Growth Factor Level are indicated with absorbance value；The results are shown in Table 4 for analysis, and statistical method uses SPSS20.0
Software analysis, measurement data indicate that each Indexes Comparison is examined using t between control group and observation group with mean ± standard deviation, take P
< 0.05 is statistically significant for difference；
4 two groups of White Rabbit poroma Nerve Growth Factor Levels of table compare (n=6, ± s)
Note: * is compared with the control group P < 0.05.
By Fig. 7 and table 4 it is found that when the 4th week, 8 weeks, 12 weeks, experimental group White Rabbit poroma Nerve Growth Factor Level is high
In control group (P < 0.05).
4 conclusions
Bone bracket complex, which is prepared, in the present invention can promote poroma formation and repairing effect at bone defect, can promote
BMSCs transfer ability improves Type I collagen albumen, CD44 level, improves Nerve Growth Factor Level in poroma；In conclusion this
Invention bone bracket complex can promote repairing for bone defect by promoting BMSCs Osteoblast Differentiation, increasing Nerve Growth Factor Level
It is multiple.
Although above having used general explanation and specific embodiment, the present invention is described in detail, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
These modifications or improvements without departing from theon the basis of the spirit of the present invention are fallen within the scope of the claimed invention.
Claims (10)
1. a kind of preparation method of bone bracket complex, which comprises the steps of:
(a) calcium polyphosphate is ground up, sieved to obtain calcium polyphosphate amorphous powder；
(b) obtained calcium polyphosphate amorphous powder is sintered, is to slowly warm up to 580-585 DEG C of holding 100-140min, with
Cooling obtains calcium polyphosphate bone holder material afterwards；
(c) calcium polyphosphate bone holder material is mixed with icariin, then forms through 3D printing speed and is answered to get the bone bracket
It is fit.
2. preparation method according to claim 1, which is characterized in that the calcium polyphosphate bone holder material and icariin
Mass ratio be (3-5): 1.
3. preparation method according to claim 1, which is characterized in that the calcium polyphosphate amorphous powder partial size is 70-100
μm。
4. preparation method according to claim 1, which is characterized in that the sintering temperature is 500-520 DEG C, and the time is
110-120min。
5. preparation method according to claim 1, which is characterized in that the slow heating rate is 2-3 DEG C/min.
6. -5 any preparation method according to claim 1, which is characterized in that the calcium polyphosphate is made by the following method
It is standby to obtain:
By Ca (H2PO4)2·H2Calcium polyphosphate amorphous imitation frosted glass is made after O is kept the temperature, then after being warming up to melting, quenching；It incites somebody to action again
To calcium polyphosphate amorphous imitation frosted glass cleaned, the calcium polyphosphate obtained by drying.
7. preparation method according to claim 6, which is characterized in that the temperature of the heat preservation is 400-600 DEG C, and the time is
50-70min。
8. preparation method according to claim 6, which is characterized in that the quenching media be selected from air, inert gas and
Any one in water.
9. preparation method according to claim 6, which is characterized in that the solvent used that cleans is ethyl alcohol.
10. a kind of bone bracket complex, which is characterized in that the bone bracket complex is by any system of claim 1-9
Preparation Method is prepared.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111821507A (en) * | 2020-08-11 | 2020-10-27 | 四川大学 | 3D printing bone tissue engineering scaffold with slow release and osteogenesis promotion functions and preparation method and application thereof |
| CN112704582A (en) * | 2021-01-25 | 2021-04-27 | 山东建筑大学 | Preparation method of customizable regenerated porous nano-material 3D printed femoral head |
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