CN109765207A - The measuring method of trace lycopene in a kind of food liquid - Google Patents
The measuring method of trace lycopene in a kind of food liquid Download PDFInfo
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- CN109765207A CN109765207A CN201910044063.XA CN201910044063A CN109765207A CN 109765207 A CN109765207 A CN 109765207A CN 201910044063 A CN201910044063 A CN 201910044063A CN 109765207 A CN109765207 A CN 109765207A
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Abstract
The invention belongs to technical field of food detection, more particularly to a kind of measuring method of trace lycopene in food liquid, method includes the following steps: (1) prepares lycopene standard solution, the Raman light scattering spectrum of lycopene standard solution is acquired, the relational model between Raman light scattering spectrum and lycopene solution concentration is drawn；(2) simultaneously constant volume is mixed added with solvent to liquid food to be measured, measures food Raman spectrum to be measured peak information, lycopene concentration is quantified according to relational model.The method of the present invention can directly detect food liquid without complicated Sample Pretreatment Technique, reduce loss of the lycopene in pretreatment process, detection limit is up to 0.1mg/kg.
Description
Technical field
The invention belongs to technical field of food detection, and in particular to the measurement of trace lycopene in a kind of food liquid
Method.
Background technique
The various nutrients wanted in tomato containing necessary for human, wherein important includes lycopene and beta carotene.
As one of tomato natural pigment, lycopene contains 11 conjugation carbon-carbon double bonds and 2 common double bonds, constitutes one
The kind more conjugated structures of non-annularity plane, unique molecular structure make it have superpower inoxidizability, its antioxygenic property is
The decades of times of vitamin E, therefore it is widely used in anti-aging and treatment such as angiocarpy, the other related diseases of cancer.
As lycopene is using more and more extensive, the detection technique of lycopene also more and more mature and diversification, mesh
The chromatography and application of gas chromatorgraphy/mass technology of preceding research comparative maturity can quantitative determine the lycopene of low concentration, however this
A kind of detection technique is relatively high for the pre-treatment requirement of sample, needs to carry out in sample a series of pretreatment process ability
Machine testing is carried out, this not only increases testing cost, and also the same increase of uncertainty of the more testing results of step, increases
Big detection risk.In addition, chromatographic detection method be due to that will extract separation, before can only establishing one kind for each food
Processing method, the scope of application is very narrow, is unfavorable for the detection of lycopene in daily complicated and diversified food substrate.
Summary of the invention
Invention broadly provides a kind of measuring method of trace lycopene in food liquid, this method can be to liquid
State food is directly detected without complicated Sample Pretreatment Technique, reduces lycopene in pretreatment process
Loss, detection limit is up to 0.1mg/kg.Its technical solution is as follows:
Using nanogold particle as substrate, using surface enhanced Raman technique to the standard solution of lycopene and
Qualitative and quantitative detection is carried out in liquid food, using the Raman spectrogram of standard solution measured by chemical quantity technical tie-up, is built
Mathematical model between vertical related and raman spectrum strength, and then the lycopene in actual sample is quantitative determined.
The measuring method of trace lycopene in a kind of food liquid, comprising the following steps:
(1) lycopene standard solution is prepared, the Raman light scattering spectrum of lycopene standard solution is acquired, draws and draws
Relational model between graceful light scattering spectrum and lycopene solution concentration；
(2) simultaneously constant volume is mixed added with solvent to liquid food to be measured, measures food Raman spectrum to be measured peak letter
Breath, quantifies lycopene concentration according to relational model.
Preferably, excitation wavelength is used to acquire Raman light scattering spectrum for the excitation spectrum of 450-800nm.
Preferably, the method for lycopene standard solution being prepared in step (1) is to weigh lycopene in volumetric flask,
With organic solvent dissolution and constant volume, its concentration is kept in dark place at the range of 100-1000mg/L, -20 DEG C, is stored up as standard
Standby liquid, is then diluted to 0.2mg/L, 0.5mg/L, 1.0mg/ with 50% organic solvent/aqueous solution for this standard reserving solution step by step
L, 2.5mg/L, 5.0mg/L, 10mg/L obtain lycopene standard solution.
Preferably, the substrate using nanogold particle as acquisition Raman light scattering spectrum.
Preferably, after mixing simultaneously constant volume added with solvent to liquid food to be measured in step (2), 0.45 μm of filter membrane was needed.
Preferably, the organic solvent is methanol, ethyl alcohol, acetone or tetrahydrofuran.
Preferably, 0.1mg/kg is limited to the detection of lycopene in food liquid, is quantitatively limited to 0.2mg/kg.
Raman spectroscopy instrument parameter setting: 450~800nm of laser source wavelength, 5~50mW of light source power, time for exposure
5~50 seconds, exposure frequency 2~10 times, 10~100 μm of narrow peak width, 3~25 times of object lens magnification, spectra collection range
300~1800cm-1。
The present invention utilizes Surface enhanced Raman spectroscopy technology, is directly detected to food liquid without complicated sample
Product pretreatment technology collects the fingerprint Raman spectral information of lycopene, establishes trace lycopene in detection food liquid
Method, lay a good foundation for the scientifical use of lycopene, also the detection for other carotenoid provides reference
Foundation.
Using the above scheme, the invention has the following advantages that
Of less demanding to matrix matching suitable for the detection of a variety of food liquids, food liquid can be analyzed directly
Without carrying out complicated pre-treatment step, reduce loss of the lycopene in pretreatment process, detection limit is reachable
0.1mg/kg.This method has detection cycle short, the low and quantitatively accurate and reliable advantage of detection limit.
Detailed description of the invention
Fig. 1 is the Fingerprint figure measured in embodiment 1.
Specific embodiment
Experimental method in following embodiment is conventional method unless otherwise required, related experiment reagent and material
Material is conventional biochemical reagent and material unless otherwise required.
Embodiment 1
1) standard solution is prepared: being weighed the lycopene of 25mg (being accurate to 0.1mg) in 25mL volumetric flask, is used acetone
Simultaneously constant volume is dissolved, its concentration is kept in dark place at the range of 1000mg/L, -20 DEG C, as standard reserving solution.Then by this
Standard reserving solution is diluted to 0.2mg/L, 0.5mg/L, 1.0mg/L, 2.5mg/L, 5.0mg/ with 50% acetone/water solution step by step
L, 10mg/L, it is to be measured；
2) processing of sample: weighing the food liquid of 2g in the volumetric flask of 10ml, adds 50% acetone/water of 5ml, is vortexed
It mixes, and with 50% acetone/water constant volume, sample liquid crosses to be measured, two parts of parallel sample preparation after 0.45 μm of filter membrane；
3) measure: respectively to 6 kinds of standard solution in 1) and 2) in the sample handled well carry out Raman spectroscopy, i.e.,
It takes 1~100 μ L prepare liquid to drop to a nanometer gold surface, carries out Raman spectrum analysis immediately after solvent volatilization.It analyzes and collects
The Raman spectrum peak information of lycopene, qualitative according to fingerprint Raman spectrum wavelength, spectrum peak areas and solution concentration are closed
System is quantified.
Wherein, Raman spectroscopy instrument parameter setting: laser source wavelength 515.5nm, 25 mW of light source power, time for exposure
10 seconds, exposure frequency 6 times, 20 μm of narrow peak width, 10 times of object lens magnification, 300~1800cm of spectra collection range-1, fingerprint
Spectrum is respectively 623nm, 1279nm, 1360nm, 1510nm, 1650nm, as shown in Figure 1.
This method can detect that the detection of the lycopene in food liquid is limited to 0.1mg/kg, quantitatively be limited to 0.2 mg/kg.
Preceding processing and drawing are carried out by above-mentioned steps by adding certain density lycopene in different food liquids
Graceful spectral detection is measured in parallel 6 parts, assesses the detection accuracy and precision of this method, measurement result is shown in Table 1.
TIANZHU XINGNAO Capsul and precision detection data (n=6) in 1. different liquid foods of table
As can be seen from the above table, using the content of lycopene in Raman spectroscopy measurement different liquids food, the rate of recovery
Greater than 85%, for relative standard deviation less than 10%, the rate of recovery and precision of method are good, can satisfy the need of coherent detection
It asks.
It will be apparent to those skilled in the art that can make various other according to the above description of the technical scheme and ideas
Corresponding change and deformation, and all these changes and deformation all should belong to the protection model of the claims in the present invention
Within enclosing.
Claims (7)
1. the measuring method of trace lycopene in a kind of food liquid, it is characterised in that: the following steps are included:
(1) lycopene standard solution is prepared, the Raman light scattering spectrum of lycopene standard solution is acquired, Raman light is drawn and dissipates
Penetrate the relational model between spectrum and lycopene solution concentration；
(2) simultaneously constant volume is mixed added with solvent to liquid food to be measured, measures food Raman spectrum to be measured peak information, according to
Relational model quantifies lycopene concentration.
2. the measuring method of trace lycopene in food liquid according to claim 1, it is characterised in that: using excitation
The excitation spectrum that wavelength is 450-800nm acquires Raman light scattering spectrum.
3. the measuring method of trace lycopene in food liquid according to claim 1, it is characterised in that: step (1)
The middle method for preparing lycopene standard solution is to weigh lycopene in volumetric flask, with organic solvent dissolution and constant volume, is made
Its concentration is kept in dark place at -20 DEG C, in the range of 100-1000mg/L as standard reserving solution, then by this standard reserving solution
It is diluted to 0.2mg/L, 0.5mg/L, 1.0mg/L, 2.5mg/L, 5.0mg/L, 10mg/ step by step with 50% organic solvent/aqueous solution
L obtains lycopene standard solution.
4. the measuring method of trace lycopene in food liquid according to claim 1, it is characterised in that: use nanometer
Substrate of the gold particle as acquisition Raman light scattering spectrum.
5. the measuring method of trace lycopene in food liquid according to claim 1, it is characterised in that: step (2)
In to liquid food to be measured added with solvent mix and constant volume after, needed 0.45 μm of filter membrane.
6. the measuring method of trace lycopene in food liquid according to claim 3, it is characterised in that: described organic
Solvent is methanol, ethyl alcohol, acetone or tetrahydrofuran.
7. the measuring method of trace lycopene in food liquid according to claim 1, it is characterised in that: eaten to liquid
The detection of lycopene is limited to 0.1mg/kg in product, is quantitatively limited to 0.2mg/kg.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113655050A (en) * | 2021-08-17 | 2021-11-16 | 南京富岛信息工程有限公司 | Method for improving Raman spectrum detection limit of trace crude oil in light oil |
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| CN102095715A (en) * | 2010-12-03 | 2011-06-15 | 江南大学 | Surface enhanced Raman spectrum detection method for antioxidant BHT (Butylated Hydroxytoluene) |
| CN105675582A (en) * | 2016-03-22 | 2016-06-15 | 浙江大学 | Method using Raman spectrum to detect beta-carotene content in fruit |
| CN109060768A (en) * | 2018-10-23 | 2018-12-21 | 江南大学 | A method of based on Surface enhanced Raman spectroscopy trace detection erythrosine concentration |
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| CN102095715A (en) * | 2010-12-03 | 2011-06-15 | 江南大学 | Surface enhanced Raman spectrum detection method for antioxidant BHT (Butylated Hydroxytoluene) |
| CN105675582A (en) * | 2016-03-22 | 2016-06-15 | 浙江大学 | Method using Raman spectrum to detect beta-carotene content in fruit |
| CN109060768A (en) * | 2018-10-23 | 2018-12-21 | 江南大学 | A method of based on Surface enhanced Raman spectroscopy trace detection erythrosine concentration |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113655050A (en) * | 2021-08-17 | 2021-11-16 | 南京富岛信息工程有限公司 | Method for improving Raman spectrum detection limit of trace crude oil in light oil |
| CN113655050B (en) * | 2021-08-17 | 2024-04-26 | 南京富岛信息工程有限公司 | Method for improving Raman spectrum detection limit of trace crude oil in light oil |
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Application publication date: 20190517 |