CN109762812A - Wheat growth gesture related SNP and its as target spot identification wheat growth gesture character in application - Google Patents
Wheat growth gesture related SNP and its as target spot identification wheat growth gesture character in application Download PDFInfo
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Abstract
A kind of application the invention discloses wheat growth gesture related SNP and its as target spot in identification wheat growth gesture character.A kind of method that the present invention also protects the growth potential for identifying wheat to be measured or plant height character, includes the following steps: to detect genotype of the wheat to be measured based on special SNP；The growth potential or plant height of AA genotype wheat are higher than GG genotype wheat.The special SNP is following (e1) or (e2): (e1) is located at the 36th nucleotide of DNA molecular shown in the sequence 4 of the sequence table in Wheat volatiles；(e2) it is located at the 21st nucleotide of DNA molecular shown in the sequence 5 of the sequence table in Wheat volatiles.The present invention can be used for the excellent wheat breed of growth potential, for breeding high-yield stable yields wheat breed based theoretical best in quality and provide molecule assisted Selection means.
Description
Technical field
The present invention relates to a kind of wheat growth gesture related SNP and its answering in identification wheat growth gesture character as target spot
With.
Background technique
Size of population expansion aggravates with extreme climate, and the traditional breeding method period is many and diverse tediously long, and grain-production and demand are lacked of proper care, and makes
It obtains crop breeding work and faces immense pressure.Crop breeding is a probabilistic process, available strain under different breeding environment
It is more, it is bigger to select preponderant genotype probability.Large-scale breeding population phenotype under how quick, accurate dynamic acquisition varying environment
Parameter, and be associated analysis with genotype, environmental factor parameter, the specific breeding objective genotype of breeding oneself become modern breeding
With the urgent need of phenomics.
Past 20 years, developed by molecule spectral technology and traditional gene sequencing technology greatly pushed Research of Plant Genomics to develop, high
Flux gene sequencing, molecular mark and genome selection technique application further speed up breeding process.With data
Acquisition platform, sensor and data processing and analysis method development, high-throughput phenotype test technology are constantly introduced into crop breeding
Research field.Multiple sensors are carried using unmanned aerial vehicle platform and obtain high-resolution field crops image data, in conjunction with digitized map
As processing technique extraction crop phenotype information and phenotypic data analysis method, it is widely used in obtaining various phenotypic parameters, wraps
Include prediction crop overground part yield and aging rate, vegetation normalization index, canopy surface temperature, plant spacing etc..High-throughput field
Crop phenotype measures correlated traits, closer to breeding practice, is conducive to assessment genotype, environmental factor and its interaction to phenotype
It influences, effectively improve breeding efficiency and assists shortening breeding process.
Wheat Johnson & Johnson growing way is conducive to improve fertility early period biomass accumulation, leaf area, Root morphology to water resource, nutrient
Utilization efficiency promotes the plant later period to develop and improve yield.Seedling stage group's plant height is the importance for identifying wheat growth gesture
Shape influences plant type, planting density, harvest index and coefficient resistant to lodging, final to determine that grain yield is formed.Plant Height in Wheat is by product
The multifactor coordinated regulation such as kind, genotype, environment is the complicated quantitative character of controlled by multiple genes, mainly by additivity, epistasis and
Environment interaction effect regulation.So far, in field experiment and laboratory test using handheld instrument to root system, leaf area,
Biomass, plant height etc. have carried out more research.Wherein, it is had found by mutant binding molecule biological means 25 short
Stalk gene (Rht).Most of dwarf genes have been exploited and have developed the molecular labeling application of close linkage therewith in wheat
In marker assisted selection breeding, wherein Rht-B1, Rht-D1 and Rht8 dwarf gene are worldwide most widely used.
Rht-B1 and Rht-D1 gene on 4B and 6D chromosome belongs to gibberellin insensitive gene, has to the elongation of wheat stipes
Great influence；Rht8 is located on 2D chromosome, belongs to Brassinosteroids insensitive gene；Carry the importing in the site of short stem Rht8
System downgrades than control strain growth potential, and spike length shortens, and spikelet number is unchanged, to generate dense cluster type phenotype.By high pass scale
Type determination techniques are more rare with the site of growth potential gene-correlation for wheat fertility early stage, and the available label of exploitation breeding is more
It is fewer and fewer.Therefore, the quantitative trait locus that new genetic effect value stablizes expression and close linkage is excavated, to molecular labeling
The excellent wheat breed of assist-breeding is of great significance.
Zhong Mai895Shi Institute of Crop Science, Chinese Academy of Agricultural Science, the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute are with all wheats
16 be it is maternal, Li Ken 4 be semi-winterness multi-spike-type middle-late ripening variety made of paternal hybrid breeding.In 2013-2015 kind
The features such as extensively suitable high yield, later period high temperature resistant of being in the milk are shown in comparative test and field demonstration.Raising wheat 16 is middle and lower reach of Yangtze River wheat
Cultivated area maximum kind in area's has the characteristics that filling speed is fast, grain weight is high.
Summary of the invention
The object of the present invention is to provide a kind of wheat growth gesture related SNP and its as target spot in identification wheat growth gesture
Application in shape.
The present invention provides special primer groups, are made of primer A, primer B and primer C；
Primer A is following (a1) or (a2):
(a1) single strand dna shown in the sequence 1 of sequence table；
(a2) sequence 1 is had by the substitution and/or deletion and/or addition of one or several nucleotide and with sequence 1
The DNA molecular of identical function；
Primer B is following (b1) or (b2):
(b1) single strand dna shown in the sequence 2 of sequence table；
(b2) sequence 2 is had by the substitution and/or deletion and/or addition of one or several nucleotide and with sequence 2
The DNA molecular of identical function；
Primer C is following (c1) or (c2):
(c1) single strand dna shown in the sequence 3 of sequence table；
(c2) sequence 3 is had by the substitution and/or deletion and/or addition of one or several nucleotide and with sequence 3
The DNA molecular of identical function.
The present invention also protects the application of the primer sets, for following (d1) or (d2) or (d3) or (d4) or (d5) or (d6)
Or (d7) or (d8) or (d9) or (d10) or (d11) or (d12) or (d13) or (d14):
(d1) genotype of the wheat based on special SNP is identified；
(d2) identify or assist the growth potential character of identification wheat；
(d3) identify or assist the plant height character of identification wheat；
(d4) wheat single plant or strain or strain or kind with low growth potential of screening or breeding；
(d5) wheat single plant or strain or strain or kind with Seedling height gesture of screening or breeding；
(d6) screening or the lower wheat single plant of breeding plant height or strain or strain or kind；
(d7) screening or the higher wheat single plant of breeding plant height or strain or strain or kind；
(d8) product for identifying genotype of the wheat based on special SNP is prepared；
(d9) product for identifying or assisting the growth potential character of identification wheat is prepared；
(d10) product for identifying or assisting the plant height character of identification wheat is prepared；
(d11) preparation is used to screen or breeding has the wheat single plant of low growth potential or the production of strain or strain or kind
Product；
(d12) preparation is used to screen or breeding has the wheat single plant of Seedling height gesture or the production of strain or strain or kind
Product；
(d13) preparation is for screening or the product of the lower wheat single plant of breeding plant height or strain or strain or kind；
(d14) preparation is for screening or the product of the higher wheat single plant of breeding plant height or strain or strain or kind.
The present invention also protects a kind of kit, including the special primer group.
The kit further includes special primer group.The specific probe group is visited by fluorescence probe A, quenching probes A, fluorescence
Needle B and quenching probes B composition；For fluorescence probe A as shown in the sequence 6 of sequence table, 5 ' ends connect fluorophor；Fluorescence probe B
As shown in the sequence 7 of sequence table, 5 ' ends connect fluorophor；Fluorophor in fluorescence probe A and fluorescence probe B is different；
For quenching probes A as shown in the sequence 8 of sequence table, 3 ' ends connect quenching group；Quenching probes B as shown in the sequence 9 of sequence table,
3 ' ends connect quenching group.Fluorescence probe A specifically connects FAM fluorophor.Fluorescence probe B specifically connects HEX fluorophor.
Quenching probes A specifically connects quenching group BHQ.Quenching probes B specifically connects quenching group BHQ.
The kit further includes 2 × Master of KASP Mix.
The present invention also protects the application of the kit, for following (d1) or (d2) or (d3) or (d4) or (d5) or (d6)
Or (d7):
(d1) genotype of the wheat based on special SNP is identified；
(d2) identify or assist the growth potential character of identification wheat；
(d3) identify or assist the plant height character of identification wheat；
(d4) wheat single plant or strain or strain or kind with low growth potential of screening or breeding；
(d5) wheat single plant or strain or strain or kind with Seedling height gesture of screening or breeding；
(d6) screening or the lower wheat single plant of breeding plant height or strain or strain or kind；
(d7) screening or the higher wheat single plant of breeding plant height or strain or strain or kind.
A kind of method that the present invention also protects growth potential character for identifying wheat to be measured, includes the following steps: to detect to be measured
Genotype of the wheat based on special SNP；The growth potential of AA genotype wheat is higher than GG genotype wheat.The life of AA genotype wheat
Growing way is higher than GG genotype wheat and AG genotype wheat.
A kind of method that the present invention also protects plant height character for identifying wheat to be measured, includes the following steps: to detect to be measured small
McGee is in the genotype of special SNP；The plant height of AA genotype wheat is higher than GG genotype wheat.The plant height of AA genotype wheat is high
In GG genotype wheat and AG genotype wheat.
A kind of method that the present invention also protects wheat breeding includes the following steps: to detect wheat to be measured based on special SNP's
Genotype；AA genotype wheat is selected to carry out breeding.The purpose of the breeding is to obtain the kind of Seedling height gesture.The breeding
Purpose is to obtain the kind of high plant height.
The special SNP of any description above is following (e1) or (e2):
(e1) it is located at the 36th nucleotide of DNA molecular shown in the sequence 4 of the sequence table in Wheat volatiles；
(e2) it is located at the 21st nucleotide of DNA molecular shown in the sequence 5 of the sequence table in Wheat volatiles.
In any description above method, the step of detecting genotype of the wheat to be measured based on special SNP, is as follows:
(1) using the genomic DNA of wheat to be measured as template, KASP is carried out using the special primer group；
(2) after completing step (1), fluorescent scanning is carried out, determines genotype of the wheat to be measured based on special SNP.
In the method, in the reaction system of KASP, the concentration of primers F 1 and primers F 2 is 0.134 μM, and primer R's is dense
Degree is 0.336 μM.
In the method, the reaction system (5.2 μ l) of KASP: 20ng/ μ l template DNA 3.0 μ l, 2 × KASP
2.0 μ l of reaction mix, primer mix reagent 0.1 μ l, ddH2O 0.1μl。
In the method, KASP is carried out on PTC-200PCR amplification instrument.
In the method, the response procedures of KASP:
94 DEG C of initial denaturation 15min；
(the 1st cycle annealing temperature is 61 DEG C, each cycle annealing temperature decline 0.6 by 94 DEG C of denaturation 30s, annealing 60s
DEG C), 72 DEG C of extension 30s, 11 circulations；
94 DEG C of denaturation 30s, 55 DEG C of annealing 60s, 72 DEG C of extension 30s, 26 recycle；72 DEG C of extension 5min.
In the method, the method that determines genotype of the wheat to be measured based on special SNP are as follows: use Kluster Caller
Software analyzes microplate reader scan data, and the genotype for being shown as the sample of blue is that AA is homozygous, shown in red
The genotype of sample is that GG is homozygous.
In the method, the method that determines genotype of the wheat to be measured based on special SNP are as follows: use Kluster Caller
Software analyzes microplate reader scan data, and the genotype for being shown as the sample of blue is that AA is homozygous, shown in red
The genotype of sample is that GG is homozygous, and the genotype of shown in green sample is AG heterozygous.
The present invention also protects a kind of specific DNA molecular, as shown in the sequence 4 of sequence table or the sequence 5 of sequence table.
The present invention also protects application of the specific DNA molecular in the growth potential character identified or assist identification wheat.
The present invention also protects application of the specific DNA molecular in the plant height character identified or assist identification wheat.
Any description above plant height is strain plant height.
The wheat to be measured is Ah husband, CA1055, CA1133, polling 987, capital winter No. 8, Qin Nong 731, Anhui wheat 52, Jining
16, Jimai 21, Shandong wheat 15, Lip river wheat 21, river wheat 52, Mianyang 26, Ning Mai 9, town wheat No. 6, middle wheat 895 or wheat 16 is raised.
The invention discloses KASP relevant to wheat growth gesture label and its applications.It is marked using KASP of the invention special
It can be used for identifying that genotype of the wheat to be measured based on special SNP is AA, GG or AG with primer, and according to the gene of wheat to be measured
Type can assist identifying its growth potential: the growth potential of the wheat to be measured of AA genotype is greater than the wheat to be measured of AG or GG genotype,
And then can be used for the excellent wheat breed of growth potential, be breeding high-yield stable yields wheat breed based theoretical best in quality and
Molecule assisted Selection means are provided.
Detailed description of the invention
Fig. 1 is the linkage map of SNP marker and QPH.caas-6DS gene.
Fig. 2 is the result figure for detecting the genotype of different cultivars wheat SNP site.
Specific embodiment
Embodiment below facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiments
Method is unless otherwise specified conventional method.Test material as used in the following examples is unless otherwise specified certainly
What routine biochemistry reagent shop was commercially available.Quantitative test in following embodiment is respectively provided with three repeated experiments, as a result makes even
Mean value.Raise wheat 16: Chinese agricultural development Group Co., Ltd, kind power number: CNA20030436.4.Wheat 895: Henan newly big agriculture
Industry Development Co., Ltd, state examine wheat 2012010.In the sequence 4 of sequence table, R represents A or G.In the sequence 5 of sequence table, R represents A
Or G.
The acquisition of embodiment 1, KASP relevant to wheat growth gesture character label primer special
One, the investigation of growth potential character and SNP marker analysis
It selects to raise wheat 16, middle wheat 895 and raise the DH group (including parent totally 200 familys) of wheat 16 and middle wheat 895 and make
For experimental material.
1, growth potential phenotype obtains
Under field conditions, the Aerial Images that platform obtains high-resolution hillslope processes are closely felt using unmanned plane.With
Pix4DMapper software (Pix4D, Switzerland) carries out processing and three-dimensional reconstruction to Aerial Images, generates entire sample plot
Just penetrate stitching image and DSM carry out geographical location and correct.These three-dimensional reconstruction output results all have horizontal position (warp
Latitude) information, wherein just penetrating color and elevation information that stitching image and DSM carry each reconstruction point respectively.By test block
Arrangement, is divided into single subdistrict for entire sample plot DSM.Based on each cell DSM, existing two kinds of main stream approach (point cloud is utilized
Method and referring to ground method) each cell growth potential of estimation.
The definition of plant height (H) be plant coboundary (upper boundary, U) and ground elevation (ground level,
G the shortest distance between), i.e. H=U-G.
2, SNP marker is analyzed
660k SNP chip is carried out to the DH group for raising wheat 895 in wheat 16/ using Illumina SNP genotyping platform
Parting amounts to 630518 including BS, BobWhite, CAP, D_contig etc..Boao Biological Co., Ltd: CapitalBio
Corporation,Beijing,China；http://bioservices.capitalbio.com.Key step: 1) will be to be measured
The genomic DNA of wheat carries out whole genome amplification, obtains amplified production；2) random endonuclease digestion disconnectedization of amplified production,
Obtain DNA fragmentation；3) DNA fragmentation is hybridized with chip, the capture of 50-mers length specificity is connected on the microballon of chip
Probe, DNA fragmentation is in conjunction with probes complementary；4) cleaning removes DNA fragmentation on non-hybridized or on mismatch hybridization；5) dinitro
The nucleotides substrate (A/T and C/G) of phenol (dinitrophenol) and biotin (biotin) label carries out single on capture probe
Base extends, and the probe of combination only complementary with gDNA generation can just be extended；By dyeing, A/T and C/G will be marked respectively
Different fluorescent dyes；6) chip scanning, and according to two kinds of fluorescence interpretations and genotyping result is exported using software.
The DH population of 626276 SNP markers wheat 895 in raising wheat 16/ is in difference.
Two, the discovery of associated gene positioning and linked marker AX-109983482
Basic statistics amount is carried out using SAS9.2 software (SAS Institute.2000), Multiple range test is analyzed, and combined
The Glmselect program of SAS carries out successive Regression to SNP data and plant height character, judges associated bit according to P value (P < 0.01)
Point.It orients AX-109983482 and is associated with (P < 0.001) with site QPH.caas-6DS.
Special SNP and its peripheral sequence are as shown in the sequence 4 of sequence table in Wheat volatiles, wherein the 36th is special
SNP site is that A/G is polymorphic.Special SNP site is also known as the site AX-109983482.
Three, the allele specific Marker Identification in the site AX-109983482
12 complete genome DNAs for raising the DH group of wheat 895 in wheat 16/ are extracted respectively.Using each genomic DNA as template
Genotype of the wheat based on the site AX-109983482 is detected, the results are shown in Table 1.The result shows that: it is based on the site AX-109983482
Genotype be AA when, wheat breed have compared with Seedling height gesture, illustrate that the site AX-109983482 can effectively identify wheat product
Kind growth potential.
1 AX-109983482 of table label allelic variation is raising the separation in 16/ Zhong Mai 895DH group of wheat
According to Wheat DArT maps Version 1.2 (http://www.triticarte.com.au) and Allen
Label AX-109983482 is integrated on wheat genetic map, as a result such as Fig. 1 institute by the wheat molecular marker map of equal announcements
Show.Determine 18.66Mb position of the QPH.caas-6DS on chromosome 6D.
Four, KASP marks the exploitation of primer special
Primer special, i.e. special primer group are marked for the site AX-109983482 KASP, by 2 upstream primer (primers
F1 and primers F 2) and 1 downstream primer (primer R) composition.Such as sequence table of target sequence of the special primer group in Wheat volatiles
Sequence 5 shown in.
Primers F 1 (sequence 1): 5 '-GAAGGTGACCAAGTTCATGCTGGAAGATTATCGGAGCTGGCA-3'；
Primers F 2 (sequence 2): 5 '-GAAGGTCGGAGTCAACGGATTGGAAGATTATCGGAGCTGGCG-3'；
Primer R:(sequence 3): 5 '-CCCACCGAACTAAACACCCA-3 '.
Primers F 1 combined with primer R can expand AX-109983482 loci gene type be AA segment, primers F 2 with draw
Object R combination can expand the segment that AX-109983482 loci gene type is GG.The sequence life of underscore mark in primers F 1
Entitled FAM fluorescence sequence, in primers F 2 in the sequence designations of underscore mark be HEX fluorescence sequence.
The application of embodiment 2, SNP
Test group is for trying wheat: A Fu, CA1055, CA1133, polling 987, capital winter No. 8, Qin Nong 731, Anhui wheat 52, Jining
16, Jimai 21, Shandong wheat 15, Lip river wheat 21, river wheat 52, Mianyang 26, Ning Mai 9, town wheat No. 6.Control group is for trying wheat: middle wheat 895
With raise wheat 16.
One, the growth potential phenotype of wheat is detected
Obtain each strain plant height for trying wheat.
The measurement of wheat growth gesture was carried out in 2018 on the farm Chinese Academy of Agricultural Sciences Dong Pu.Under field conditions, use
Unmanned plane closely feels the Aerial Images that platform obtains high-resolution hillslope processes.With Pix4DMapper software (Pix4D,
Switzerland) to Aerial Images carry out processing and three-dimensional reconstruction, generate entire sample plot just penetrate stitching image and DSM into
Row geographical location simultaneously corrects.These three-dimensional reconstruction output results all have horizontal position (longitude and latitude) information, wherein just penetrating spelling
Map interlinking picture and DSM carry the color and elevation information of each reconstruction point respectively.It is arranged by test block, by entire sample plot DSM
It is divided into single subdistrict.Based on each cell DSM, each cell growth potential is estimated using referring to ground method.The definition of plant height (H) is to plant
The shortest distance between the coboundary (upper boundary, U) and ground elevation (ground level, G) of strain, i.e. H=U-G.
Two, the genotype of different cultivars wheat SNP site is detected
It detects each for trying genotype of the wheat based on special SNP.
The genomic DNA for trying wheat is extracted, using genomic DNA as template, using the KASP in the step of embodiment 1 four
Primer special is marked to carry out KASP.Wherein, the amplified production for carrying fluorescence sequence FAM is displayed in blue through fluorescence irradiation, and is carried
The amplified production of fluorescence sequence HEX is displayed in red through fluorescence irradiation.
Reaction system (total volume is 5.2 μ l): 3.0 μ l, 2 × KASP reaction mix 2.0 of 20ng/ μ l template DNA
μ l, primer mix reagent (Assay mix) 0.1 μ l, ddH2O 0.1μl.2 × KASP reaction mix, also known as KASP 2
× Master Mix is LGC Products (article No. KBS-1016-002).2 × KASP reaction mix includes fluorescence probe
A, the Taq enzyme of fluorescence probe B, quenching probes A and quenching probes B and high-fidelity, dNTP etc..The sequence of fluorescence probe A is
5 '-GAAGGTGACCAAGTTCATGCT-3 ', 5 ' ends connect 1 fluorophor FAM；The sequence of fluorescence probe B is 5 '-
GAAGGTCGGAGTCAACGGATT-3 ', 5 ' ends connect 1 fluorophor HEX；The sequence of quenching probes A is 5 '-
AGCATGAACTTGGTCACCTTC-3 ', 3 ' ends connect quenching group BHQ；The sequence of quenching probes B is 5 '-
AATCCGTTGACTCCGACCTTC-3 ', 3 ' ends connect quenching group BHQ.The effective component that primer mix reagent provides is to draw
Object F1, primers F 2 and primer R.In reaction system, the concentration of primers F 1 and primers F 2 is 0.134 μM, and the concentration of primer R is
0.336μM。
Reaction carries out on PTC-200PCR amplification instrument, using Touch down PCR amplification program.Response procedures are as follows: 94
DEG C initial denaturation 15min；(the 1st cycle annealing temperature is 61 DEG C, at a temperature of each cycle annealing by 94 DEG C of denaturation 30s, annealing 60s
0.6 DEG C of drop), 72 DEG C of extension 30s, 11 circulations；94 DEG C of denaturation 30s, 55 DEG C of annealing 60s, 72 DEG C of extension 30s, 26 recycle；72
DEG C extend 5min；10 DEG C of preservations.
By reaction product in PHERAstarplusGenotyping is carried out with fluorescence irradiation on fluorescence microplate reader, is then existed
KlusterCallerTMSoftware reads the data after parting, is only displayed in blue the gene that image then illustrates the site AX-109983482
Type is AA；It is only displayed in red image and then illustrates that the genotype in the site AX-109983482 is GG；Display green image then illustrates AX-
The genotype in 109983482 sites is AG.
Partial results are shown in Fig. 2.
Three, the relationship of genotype and character
The plant height character (growth potential) for each wheat that step 2 obtains the genotype of each wheat and its step 1 obtains
It is shown in Table 2.
The genotype and growth potential result of the SNP site of 2 wheat breed of table
The above result shows that: A Fu, capital winter No. 8, Shandong wheat 15, Lip river wheat 21, river wheat 52, Mianyang 26, Ning Mai 9, town wheat No. 6
It is AA with genotype of the wheat 16 in SNP site is raised, and these wheats all have higher growth potential.May be used also in from the above
Find out, the growth potential for the wheat that the genotype of SNP site is AA is higher than the wheat that genotype is GG.The above results show above-mentioned
SNP site can quickly and accurately identify wheat breed growth potential whether with higher.
It can establish following methods: A wheat (AX-109983482 loci gene type is the homozygote of A, genotype AA)
Growth potential be greater than B wheat (genotype is GG or genotype is AG).
It can establish following methods: A wheat (AX-109983482 loci gene type is the homozygote of A, genotype AA)
Plant height be greater than B wheat (genotype is GG or genotype is AG).
SEQUENCE LISTING
<110>Institute of Crop Science, Chinese Academy of Agricultural Science
<120>wheat growth gesture related SNP and its application as target spot in identification wheat growth gesture character
<130> GNCYX190139
<160> 9
<170> PatentIn version 3.5
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Claims (10)
1. special primer group is made of primer A, primer B and primer C；
Primer A is following (a1) or (a2):
(a1) single strand dna shown in the sequence 1 of sequence table；
(a2) have by sequence 1 by the substitution and/or deletion and/or addition of one or several nucleotide and with sequence 1 identical
The DNA molecular of function；
Primer B is following (b1) or (b2):
(b1) single strand dna shown in the sequence 2 of sequence table；
(b2) have by sequence 2 by the substitution and/or deletion and/or addition of one or several nucleotide and with sequence 2 identical
The DNA molecular of function；
Primer C is following (c1) or (c2):
(c1) single strand dna shown in the sequence 3 of sequence table；
(c2) have by sequence 3 by the substitution and/or deletion and/or addition of one or several nucleotide and with sequence 3 identical
The DNA molecular of function.
2. the application of primer sets described in claim 1, for following (d1) or (d2) or (d3) or (d4) or (d5) or (d6) or
(d7) or (d8) or (d9) or (d10) or (d11) or (d12) or (d13) or (d14):
(d1) genotype of the wheat based on special SNP is identified；
(d2) identify or assist the growth potential character of identification wheat；
(d3) identify or assist the plant height character of identification wheat；
(d4) wheat single plant or strain or strain or kind with low growth potential of screening or breeding；
(d5) wheat single plant or strain or strain or kind with Seedling height gesture of screening or breeding；
(d6) screening or the lower wheat single plant of breeding plant height or strain or strain or kind；
(d7) screening or the higher wheat single plant of breeding plant height or strain or strain or kind；
(d8) product for identifying genotype of the wheat based on special SNP is prepared；
(d9) product for identifying or assisting the growth potential character of identification wheat is prepared；
(d10) product for identifying or assisting the plant height character of identification wheat is prepared；
(d11) preparation is used to screen or breeding has the wheat single plant of low growth potential or the product of strain or strain or kind；
(d12) preparation is used to screen or breeding has the wheat single plant of Seedling height gesture or the product of strain or strain or kind；
(d13) preparation is for screening or the product of the lower wheat single plant of breeding plant height or strain or strain or kind；
(d14) preparation is for screening or the product of the higher wheat single plant of breeding plant height or strain or strain or kind；
The special SNP is following (e1) or (e2):
(e1) it is located at the 36th nucleotide of DNA molecular shown in the sequence 4 of the sequence table in Wheat volatiles；
(e2) it is located at the 21st nucleotide of DNA molecular shown in the sequence 5 of the sequence table in Wheat volatiles.
3. a kind of kit, including special primer group described in claim 1.
4. the application of kit described in claim 3, for following (d1) or (d2) or (d3) or (d4) or (d5) or (d6) or
(d7):
(d1) genotype of the wheat based on special SNP is identified；
(d2) identify or assist the growth potential character of identification wheat；
(d3) identify or assist the plant height character of identification wheat；
(d4) wheat single plant or strain or strain or kind with low growth potential of screening or breeding；
(d5) wheat single plant or strain or strain or kind with Seedling height gesture of screening or breeding；
(d6) screening or the lower wheat single plant of breeding plant height or strain or strain or kind；
(d7) screening or the higher wheat single plant of breeding plant height or strain or strain or kind；
The special SNP is following (e1) or (e2):
(e1) it is located at the 36th nucleotide of DNA molecular shown in the sequence 4 of the sequence table in Wheat volatiles；
(e2) it is located at the 21st nucleotide of DNA molecular shown in the sequence 5 of the sequence table in Wheat volatiles.
5. a kind of method for the growth potential character for identifying wheat to be measured includes the following steps: that detecting wheat to be measured is based on special SNP
Genotype；The growth potential of AA genotype wheat is higher than GG genotype wheat；
The special SNP is following (e1) or (e2):
(e1) it is located at the 36th nucleotide of DNA molecular shown in the sequence 4 of the sequence table in Wheat volatiles；
(e2) it is located at the 21st nucleotide of DNA molecular shown in the sequence 5 of the sequence table in Wheat volatiles.
6. a kind of method for the plant height character for identifying wheat to be measured, includes the following steps: to detect wheat to be measured based on special SNP's
Genotype；The plant height of AA genotype wheat is higher than GG genotype wheat；
The special SNP is following (e1) or (e2):
(e1) it is located at the 36th nucleotide of DNA molecular shown in the sequence 4 of the sequence table in Wheat volatiles；
(e2) it is located at the 21st nucleotide of DNA molecular shown in the sequence 5 of the sequence table in Wheat volatiles.
7. a kind of method of wheat breeding includes the following steps: to detect genotype of the wheat to be measured based on special SNP；Select AA
Genotype wheat carries out breeding；
The special SNP is following (e1) or (e2):
(e1) it is located at the 36th nucleotide of DNA molecular shown in the sequence 4 of the sequence table in Wheat volatiles；
(e2) it is located at the 21st nucleotide of DNA molecular shown in the sequence 5 of the sequence table in Wheat volatiles.
8. specific DNA molecular；The specific DNA molecular is as shown in the sequence 4 of sequence table or the sequence 5 of sequence table.
9. application of the specific DNA molecular described in claim 8 in the growth potential character identified or assist identification wheat.
10. application of the specific DNA molecular described in claim 8 in the plant height character identified or assist identification wheat.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110616276A (en) * | 2019-11-04 | 2019-12-27 | 中国农业科学院作物科学研究所 | Method for assisting in identifying surface area characters of wheat seedling-stage roots and special primer group thereof |
| CN111979345A (en) * | 2020-08-07 | 2020-11-24 | 中国科学院遗传与发育生物学研究所 | Development and application of KASP (Kaempferi protein) marker related to wheat biomass and yield under salt stress condition |
| CN112175812A (en) * | 2020-10-13 | 2021-01-05 | 山西农业大学 | Fluid control device for researching genetic diversity of pigs based on high-throughput gene sequencing |
| CN116606916A (en) * | 2023-06-30 | 2023-08-18 | 河北师范大学 | Method for identifying, screening or controlling wheat spike number per spike based on SNP locus |
| WO2023232152A1 (en) * | 2022-08-24 | 2023-12-07 | 江苏省农业科学院 | Multiplex kasp marker primer set for wheat plant height major genes and use thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105821038A (en) * | 2016-05-24 | 2016-08-03 | 中国农业科学院作物科学研究所 | Molecular marker for identifying wheat height and specific primers |
| CN107805673A (en) * | 2017-11-20 | 2018-03-16 | 中国农业科学院作物科学研究所 | A kind of SNP marker related to Wheat Seedling root traits and application |
| CN108060262A (en) * | 2018-02-08 | 2018-05-22 | 中国农业科学院作物科学研究所 | KASP marks relevant with wheat root character and its application |
-
2019
- 2019-03-05 CN CN201910162817.1A patent/CN109762812B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105821038A (en) * | 2016-05-24 | 2016-08-03 | 中国农业科学院作物科学研究所 | Molecular marker for identifying wheat height and specific primers |
| CN107805673A (en) * | 2017-11-20 | 2018-03-16 | 中国农业科学院作物科学研究所 | A kind of SNP marker related to Wheat Seedling root traits and application |
| CN108060262A (en) * | 2018-02-08 | 2018-05-22 | 中国农业科学院作物科学研究所 | KASP marks relevant with wheat root character and its application |
Non-Patent Citations (2)
| Title |
|---|
| XINGMAO LI等: "QTL mapping for plant height and yield components in common wheat under water-limited and full irrigation environments", 《CROP & PASTURE SCIENCE》 * |
| 刘静等: "小麦株高性状的全基因组关联分析", 《第八届全国小麦基因组学及分子育种大会摘要集》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110616276A (en) * | 2019-11-04 | 2019-12-27 | 中国农业科学院作物科学研究所 | Method for assisting in identifying surface area characters of wheat seedling-stage roots and special primer group thereof |
| CN110616276B (en) * | 2019-11-04 | 2022-07-19 | 中国农业科学院作物科学研究所 | Method for assisting in identifying surface area characters of wheat seedling-stage roots and special primer group thereof |
| CN111979345A (en) * | 2020-08-07 | 2020-11-24 | 中国科学院遗传与发育生物学研究所 | Development and application of KASP (Kaempferi protein) marker related to wheat biomass and yield under salt stress condition |
| CN112175812A (en) * | 2020-10-13 | 2021-01-05 | 山西农业大学 | Fluid control device for researching genetic diversity of pigs based on high-throughput gene sequencing |
| WO2023232152A1 (en) * | 2022-08-24 | 2023-12-07 | 江苏省农业科学院 | Multiplex kasp marker primer set for wheat plant height major genes and use thereof |
| CN116606916A (en) * | 2023-06-30 | 2023-08-18 | 河北师范大学 | Method for identifying, screening or controlling wheat spike number per spike based on SNP locus |
| CN116606916B (en) * | 2023-06-30 | 2023-11-28 | 河北师范大学 | Method for identifying, screening or controlling wheat spike number per spike based on SNP locus |
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