CN109762772A - Pseudomonas aeruginosa qualitative criteria sample and preparation method in water soluble cosmetics - Google Patents
Pseudomonas aeruginosa qualitative criteria sample and preparation method in water soluble cosmetics Download PDFInfo
- Publication number
- CN109762772A CN109762772A CN201910149259.5A CN201910149259A CN109762772A CN 109762772 A CN109762772 A CN 109762772A CN 201910149259 A CN201910149259 A CN 201910149259A CN 109762772 A CN109762772 A CN 109762772A
- Authority
- CN
- China
- Prior art keywords
- sample
- pseudomonas aeruginosa
- water soluble
- freeze
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention belongs to the field of quality control of Cosmetics microorganism context of detection, in particular to pseudomonas aeruginosa qualitative criteria's sample and preparation method thereof in a kind of water soluble cosmetics.Pseudomonas aeruginosa qualitative criteria sample includes object bacteria and background flora in water soluble cosmetics, and the object bacteria is pseudomonas aeruginosa, and the background flora is made of Bacillus cereus, escherichia coli, staphylococcus aureus.The composition of flora and content are consistent with actual sample in sample of the present invention；Standard sample is consistent with actual sample matrix composition；Uniformity, stability comply with standard sample requirement, can utmostly guarantee the stability of standard sample；Preparation method, the simple process of sample, success rate are high.
Description
Technical field
The invention belongs to the field of quality control of Cosmetics microorganism context of detection, in particular to a kind of water-soluble makeup
Pseudomonas aeruginosa qualitative criteria sample and preparation method thereof in product.
Background technique
Cosmetics are the personal care products in many people's daily lifes, and the effect and safety of itself are directly related to
The health safety of user.Microorganism directly reflects the degree that cosmetics are contaminated by bacterial, and production in cosmetics
Raw material used in unit, tool equipment, process flow, operator sanitary condition, be to cosmetics carry out hygienical evaluation
Compositive index.Water soluble cosmetics microbiological Test field is very special.Currently, at home and abroad there is no mechanism preparation is water-soluble
The precedent of Cosmetics microorganism standard sample and large-scale production, still belongs to blank field.It is only standard that the country is existing at present
Bacterial strain or the standard sample suitable for field of food for only containing pure bacterium, it is inconsistent with actual sample, it is unable to satisfy daily reality
Test the needs such as room quality control.
Pseudomonas aeruginosa is gram-Negative bacillus, and oxidase positive can generate pyo.Furthermore it can also liquefy bright
Glue, reduction nitrate are nitrite, can be grown under the conditions of 1 DEG C of 42 scholar.The height of background bacterium and target bacterial content is also system
For the significant consideration of the standard sample, the lower therefore prepared standard sample of content of microorganisms in usual actual sample
Middle background bacterium and target bacterial content should be consistent with actual sample.Therefore, comprehensively consider the biochemical characteristic of pseudomonas aeruginosa,
It is particularly significant to the uniformity and stability that guarantee sample as target flora to choose the metastable reference culture of property, and
The uniformity and stability and the strain of freeze-drying of sample, the process conditions of freeze-drying, the use of freeze drying protectant, rehydration medium
Deng there is close relationship.
Pseudomonas aeruginosa has pathogenicity, Chang Yinqi application on human skin suppurative infection to people.Therefore, in hygienic standards for cosmetics
Must not provide and detects pseudomonas aeruginosa.Uniformity and the qualitative sample of all good staphylococcus aureus of stability are prepared, it can be with
The randomness and uncertainty for avoiding pseudomonas aeruginosa from examining, are truly reflected the ability of testing laboratory.This is real to improving
Room quality control level is tested, ensures that the quality of water soluble cosmetics has special important meaning.
Summary of the invention
The object of the present invention is to provide a kind of and consistent standard samples of actual sample, for matter in cosmetics detection process
Purposes, uniformity, the stability such as amount control, staff training, method validation, culture medium or kit performance evaluation comply with standard sample
The requirement of product, it is a further object to provide the preparation method of the sample, simple process, success rate is high.
Present invention technical solution used for the above purpose is: pseudomonas aeruginosa is qualitative in water soluble cosmetics
Standard sample, it is characterized in that: including object bacteria and background flora, the object bacteria is pseudomonas aeruginosa
(Pseudomonasaeruginosa), the background flora is uncommon by Bacillus cereus (Bacillus cereus), large intestine angstrom
Salmonella (E.coil), staphylococcus aureus (Staphylococcus aureus) composition.
The sample matrices are toner, and makeup water volume fraction is 0.5%；Protective agent is with trehalose and sterile water group
At wherein trehalose volume fraction is 10%.
The aimed concn of object bacteria is 10 in the sample2~103CFU/mL, the aimed concn of background flora are 103CFU/
mL。
The preparation method of pseudomonas aeruginosa qualitative criteria sample in water soluble cosmetics, it is characterized in that: including that freeze-drying is protected
Preparation, sample freeze-drying, the uniformity of sample and four stability test, sample definite value steps of agent are protected, wherein sample was lyophilized
Journey are as follows: bacterial strain recovery passage-increasing bacterium-bacteria suspension preparation-freeze-drying-packaging-storage, detailed process are as follows:
(1) bacterial strain recovery passage
Reference culture is inoculated into nutrient agar slant medium, so that it is recovered and is grown at 36 ± 1 DEG C, and to multiple
The bacterial strain of Soviet Union is identified；
(2) increase bacterium
Object bacteria culture to the logarithmic growth end of term, background bacteria group culture to stationary phase scrapes bacterium colony from inclined-plane, is added to 10mL
Freeze drying protectant in the bacterium solution of corresponding single culture is made；
(3) bacteria suspension is prepared
The bacterium solution that a upper process obtains is mixed with freeze drying protectant, according to target bacteria concentration 102~103CFU/mL and background
The aimed concn 10 of flora3CFU/mL prepares bacteria suspension, prepares the bacteria suspension of target bacteria suspension and 4 background bacterium respectively, respectively
It is mixed to form target bacteria suspension and background flora suspension by 1:1 volume ratio, then target bacteria suspension and background flora suspension are pressed into 1:1
Volume ratio mixing, obtains plastc ring, is placed on magnetic stirring apparatus and is stirred continuously, and 1:100 ratio adds toner, sufficiently stirs
It is dispensed into sample bottle after mixing mixing, in addition bottle stopper, but reality is not covered there are gap；
(4) it is lyophilized and packs
Sample bottle equipped with plastc ring is uncapped and is put into freeze dryer, 50~55h is freeze-dried, when sample freezing is dry
It after dry, directly carries out jumping a queue in case, closing machine, sample is vacuum state in sample bottle；
(5) it stores
Sample is placed under the conditions of -15~-20 DEG C and is kept in dark place, random selected sample is issued to reality from whole samples
It tests room or carries out uniformity and stability test.
The sample matrices are toner, and makeup water volume fraction is 0.5%；Protective agent is with trehalose and sterile water group
At wherein trehalose volume fraction is 10%.
" standard sample works directive/guide (3) according to GB/T 15000.3-2008 for the uniformity of the sample and stability test
The rule and statistical method of standard sample definite value " it carries out.
Strain of the present invention is selected using pseudomonas aeruginosa as the qualitative sample of pseudomonas aeruginosa in water soluble cosmetics
The object bacteria of product, using Bacillus cereus, escherichia coli, staphylococcus aureus as background flora.It selects and reality
The consistent toner of sample is matrix, consistent with actual sample；The trehalose with preferable frozen-dried protective effect is selected to protect
Agent is protected, and selects in the environment such as Bacillus cereus, escherichia coli, staphylococcus aureus common bacteria as background bacterium
Group simulates competition or disturbance state in actual sample between different bacterium；The simulation of the additive amount of object bacteria and background flora is practical
The content of microorganism in sample, sufficiently plays quality control action.
Pseudomonas aeruginosa qualitative criteria sample has the advantage that characteristic in water soluble cosmetics of the invention: sample
Matrix is consistent with actual sample, and the composition of flora and content are consistent with actual sample in sample；Target bacterial content and actual sample
Unanimously；Microorganism living, quantity does not change, biochemical character does not morph, and sets about from the condition for meeting special transport, leads to
It crosses the research of special process, research of stability and uniformity etc. and forms a set of perfect standard sample technology of preparing, be suitable for
The preparation of pseudomonas aeruginosa qualitative criteria sample in water soluble cosmetics.
Detailed description of the invention
Fig. 1 is process flow chart of the invention.
Specific embodiment
With reference to the accompanying drawing and specific embodiment present invention is further described in detail, but the invention is not limited to tools
Body embodiment.
Embodiment 1
Pseudomonas aeruginosa qualitative criteria sample in water soluble cosmetics, including object bacteria and background flora, the target
Bacterium is pseudomonas aeruginosa (Pseudomonasaeruginosa), the background Bacillus cereus (Bacillus
Cereus), escherichia coli (E.coil), staphylococcus aureus (Staphylococcus aureus) composition.
The sample matrices are toner, and makeup water volume fraction is 0.5%；Protective agent is with trehalose and sterile water group
At wherein trehalose volume fraction is 10%.
The aimed concn of object bacteria is 10 in the sample2~103CFU/mL, the aimed concn of background flora are 103CFU/
mL。
The sample is for quality control, staff training examination, method validation, culture medium or examination in cosmetics detection process
Agent examination and quality control etc..Such as the culture medium that testing laboratory purchases, needs to verify its validity, can not be verified from appearance,
It then can use the standard sample, provided according to detection method, carry out culture and corresponding detection using culture medium to be verified,
As a result testing result unanimously then illustrates that culture medium can be used by checking and accepting compared with the consistency of the characteristic value of standard sample
In the detection of the project；Otherwise it then needs further to confirm.
Embodiment 2
As shown in Figure 1, in a kind of embodiment 1 in water soluble cosmetics pseudomonas aeruginosa qualitative criteria's sample preparation side
Method, preparation, sample freeze-drying, the uniformity of sample and stability test, standard sample definite value four including sample freeze drying protectant
A step, the specific steps are as follows:
1, the preparation of sample addition bacterial strain
According to object bacteria: pseudomonas aeruginosa (Pseudomonasaeruginosa), background flora: Bacillus cereus
(Bacillus cereus), escherichia coli (E.coil), staphylococcus aureus (Staphylococcus aureus)
Selection criteria bacterial strain, all reference cultures are purchased from the qualified Culture Collection Center of regular tool, and have bacterial strain certificate, protect
The traceability of bacterial strain is demonstrate,proved.
2, the preparation of freeze drying protectant
Sample matrices are toner, and makeup water volume fraction is 0.5%；Protective agent is formed with trehalose and sterile water,
Middle trehalose volume fraction is 10%.
3, sample is lyophilized
(1) bacterial strain recovery passage
Reference culture is inoculated into nutrient agar slant medium, so that it is recovered and is grown at 36 ± 1 DEG C, and to multiple
The bacterial strain of Soviet Union is identified；
(2) increase bacterium
Object bacteria culture to the logarithmic growth end of term, background bacteria group culture to stationary phase scrapes bacterium colony from inclined-plane, is added to 10mL
Freeze drying protectant in the bacterium solution of corresponding single culture is made；
(3) bacteria suspension is prepared
The bacterium solution that a upper process obtains is mixed with freeze drying protectant, according to target the aimed concn 10 of bacterium2~103CFU/mL
With the aimed concn 10 of background flora3CFU/mL prepares bacteria suspension.To guarantee to contain the above target rich in obtained final sample
The bacterial strain of degree, according to object bacteria 10 in the present embodiment3CFU/mL prepares bacteria suspension respectively, mixes by 1:1 volume ratio, forms target
Bacteria suspension；Background bacterium presses 104CFU/mL prepares the bacteria suspension of 4 background bacterium, mixes by 1:1 volume ratio, and it is outstanding to form background flora
Liquid；Background flora suspension is mixed with target flora suspension by 1:1 volume ratio, obtains plastc ring, is added according to 1:100 volume ratio
Add toner, bacterial strain content is checked using turbidimetry；Being placed on magnetic stirring apparatus to be stirred continuously down takes 1.0mL to be dispensed into sample bottle
In (cillin bottle), in addition bottle stopper, but reality is not covered there are gap；
(4) it is lyophilized and packs
Sample bottle equipped with plastc ring is uncapped and is put into freeze dryer, freeze drier parameter is by following setting:
Chilling rate (Cooling Rate) 0.5 DEG C/min
- 1 DEG C of 15min of early stage cold point (Incipient Freezing Point)
- 40 DEG C of cryogenic temperature (Cooling temperature)
- 29 DEG C of eutectic point (Melting Point Eutectic temperature)
20 DEG C of 120min of heating temperature (Heating temperature)
Start freeze drier, machine is directly entered the freezing dry process of sequencing, entire freeze-drying process 50h.
After sample freeze-drying, directly carry out jumping a queue in case；Closing machine.The not tight cillin bottle of plug is rejected,
Sample is vacuum state in cillin bottle；
(5) it stores
Sample is placed under the conditions of -15~-20 DEG C and is kept in dark place, and the sample of preservation is suitably managed and examined
It surveys, random selected sample is issued to laboratory or carries out uniformity and stability test from whole samples.
4, the uniformity and stability test of sample
Uniformity and stability inspection to object bacteria in sample are the main methods of verification sample preparation process validity.
Checking sample homogeneity and stability, " standard sample works directive/guide (3) standard sample definite value according to GB/T 15000.3-2008
Rule and statistical method " it carries out.Gained sample homogeneity and Detection of Stability method and result are as follows:
12 samples are randomly selected respectively, using cosmetics safety technical specification (2015 editions) test method, are repeating item
Part tests the pseudomonas aeruginosa of 2 × 12 parts of samples.Result data carries out statistical disposition, statistic procedure and knot with method of analysis of variance
Fruit is as follows:
1 variance analysis formula of table
In upper table,
2 sample homogeneity testing result of table
3 sample homogeneity of table tests the results of analysis of variance
Conclusion: under 95% fiducial probability, compared with other factors are to the influence of test result, the inhomogeneities of sample is
It is acceptable.
Using two kinds of stability test: one is the stability test under storage temperature (4 DEG C), another kind is
Stability test at high temperature (traffic condition of analog sample), selects three temperature spots, respectively 20 DEG C, 36 DEG C and
45℃.Periodic detection sample tests 3 samples for different temperature points different holding time, by 2 × 3 parts of sample results
Mean value (after logarithmic transformed) carries out statistical disposition, F value < F with method of analysis of varianceCritical value, then the stability of description standard sample meets
It is required that while determining the longest holding time for complying with standard sample requirement under condition of different temperatures.Stability test result is shown in
Table 4 and table 5.
4 sample short-term stability testing result of table
5 sample short-term stability of table tests the results of analysis of variance
5, standard sample definite value
According to the GB/T 15000.3-2008 " rule and statistics of standard sample work directive/guide (3) standard sample definite value
Method " relevant regulations progress, in such a way that definite value is combined in the laboratory Duo Jia, level of organization sample assignment certificate of entrustment and operation refer to
Book is led, each definite value laboratory sends 3 samples, it is desirable that laboratory uses " cosmetics safety technical specification 2015 editions " to carry out gold
The qualitative detection of staphylococcus aureus, each sample feed back 2 retests as a result, counting to the result of laboratory feedback
Macro or mass analysis determines the characteristic value of standard sample.
Embodiment 3
The preparation method of pseudomonas aeruginosa qualitative criteria sample is each in water soluble cosmetics described in the present embodiment
Step is in the same manner as in Example 2, different technical parameters are as follows: during bacteria suspension is prepared, target bacteria suspension concentration according to 5 ×
103CFU/mL is prepared, and the concentration of each background bacterium is according to 10 in background flora suspension3CFU/mL is prepared；Freeze-drying process 52h.
Embodiment 4
The preparation method of pseudomonas aeruginosa qualitative criteria sample is each in water soluble cosmetics described in the present embodiment
Step is in the same manner as in Example 3, different technical parameters are as follows: during bacteria suspension is prepared, target bacteria suspension concentration according to 4.5 ×
103CFU/mL is prepared；Freeze-drying process 55h.
Claims (6)
1. pseudomonas aeruginosa qualitative criteria sample in water soluble cosmetics, it is characterized in that: including object bacteria and background flora, institute
Stating object bacteria is pseudomonas aeruginosa, and the background flora is by Bacillus cereus, escherichia coli, staphylococcus aureus
Composition.
2. pseudomonas aeruginosa qualitative criteria sample in water soluble cosmetics according to claim 1, it is characterized in that: described
Sample matrices are toner, and makeup water volume fraction is 0.5%；Protective agent is formed with trehalose and sterile water, wherein trehalose body
Fraction is 10%.
3. pseudomonas aeruginosa qualitative criteria sample in water soluble cosmetics according to claim 1 or 2, it is characterized in that:
The aimed concn of object bacteria is 10 in the sample2~103CFU/mL, the aimed concn of background flora are 103 CFU/mL。
4. the preparation method of pseudomonas aeruginosa qualitative criteria sample in water soluble cosmetics according to claim 1,
It is characterized in: preparation, sample freeze-drying, the uniformity of sample and stability test, sample definite value four steps including freeze drying protectant
Suddenly, wherein sample freeze-drying process are as follows: bacterial strain recovery passage-increasing bacterium-bacteria suspension preparation-freeze-drying-packaging-storage, specific mistake
Journey are as follows:
(1) bacterial strain recovery passage
Reference culture is inoculated into nutrient agar slant medium, so that it is recovered and is grown at 36 ± 1 DEG C, and to recovery
Bacterial strain is identified；
(2) increase bacterium
Object bacteria culture to the logarithmic growth end of term, background bacteria group culture to stationary phase scrapes bacterium colony from inclined-plane, is added to the jelly of 10mL
The bacterium solution of corresponding single culture is made in dry protective agent；
(3) bacteria suspension is prepared
The bacterium solution that a upper process obtains is mixed with freeze drying protectant, according to target bacteria concentration 102~103CFU/mL and background flora
Aimed concn 103 CFU/mL prepares bacteria suspension, prepares the bacteria suspension of target bacteria suspension and 4 background bacterium respectively, presses 1:1 respectively
Volume ratio is mixed to form target bacteria suspension and background flora suspension, then target bacteria suspension and background flora suspension are pressed 1:1 volume
Than mixing, plastc ring is obtained, is placed on magnetic stirring apparatus and is stirred continuously, 1:100 ratio adds toner, is sufficiently stirred mixed
It is dispensed into sample bottle after even, in addition bottle stopper, but reality is not covered there are gap；
(4) it is lyophilized and packs
Sample bottle equipped with plastc ring is uncapped and is put into freeze dryer, 50 ~ 55h is freeze-dried, is tied when sample is freeze-dried
Shu Hou directly carries out jumping a queue in case, closing machine, and sample is vacuum state in sample bottle；
(5) it stores
Sample is placed under the conditions of -15 ~ -20 DEG C and is kept in dark place, random selected sample is issued to laboratory from whole samples
Or carry out uniformity and stability test.
5. the preparation method of pseudomonas aeruginosa qualitative criteria sample in water soluble cosmetics according to claim 4,
Be characterized in: the sample matrices are toner, and makeup water volume fraction is 0.5%；Protective agent is formed with trehalose and sterile water,
Wherein trehalose volume fraction is 10%.
6. the preparation method of pseudomonas aeruginosa qualitative criteria sample in water soluble cosmetics according to claim 4,
Be characterized in: the uniformity of the sample and stability test are according to GB/T 15000.3-2008 " standard sample work directive/guide (3)
The rule and statistical method of standard sample definite value " it carries out.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910149259.5A CN109762772A (en) | 2019-02-28 | 2019-02-28 | Pseudomonas aeruginosa qualitative criteria sample and preparation method in water soluble cosmetics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910149259.5A CN109762772A (en) | 2019-02-28 | 2019-02-28 | Pseudomonas aeruginosa qualitative criteria sample and preparation method in water soluble cosmetics |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109762772A true CN109762772A (en) | 2019-05-17 |
Family
ID=66456521
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910149259.5A Pending CN109762772A (en) | 2019-02-28 | 2019-02-28 | Pseudomonas aeruginosa qualitative criteria sample and preparation method in water soluble cosmetics |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109762772A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111662949A (en) * | 2020-07-13 | 2020-09-15 | 北京海关技术中心 | Sample for verifying qualitative detection capability of pseudomonas aeruginosa in mask and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103614451A (en) * | 2013-11-29 | 2014-03-05 | 中山鼎晟生物科技有限公司 | Method for detecting microorganisms of cosmetics |
| CN107142304A (en) * | 2017-05-24 | 2017-09-08 | 中国检验检疫科学研究院 | Pseudomonas aeruginosa proficiency testing sample and preparation method thereof in medicine |
-
2019
- 2019-02-28 CN CN201910149259.5A patent/CN109762772A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103614451A (en) * | 2013-11-29 | 2014-03-05 | 中山鼎晟生物科技有限公司 | Method for detecting microorganisms of cosmetics |
| CN107142304A (en) * | 2017-05-24 | 2017-09-08 | 中国检验检疫科学研究院 | Pseudomonas aeruginosa proficiency testing sample and preparation method thereof in medicine |
Non-Patent Citations (1)
| Title |
|---|
| 税丕容: "微生物检测在化妆品质量检验中的应用", 《化工管理》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111662949A (en) * | 2020-07-13 | 2020-09-15 | 北京海关技术中心 | Sample for verifying qualitative detection capability of pseudomonas aeruginosa in mask and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107190048A (en) | Staphylococcus aureus proficiency testing sample and preparation method thereof in medicine | |
| CN107142304A (en) | Pseudomonas aeruginosa proficiency testing sample and preparation method thereof in medicine | |
| CN107190046A (en) | EHEC proficiency testing sample and preparation method thereof in medicine | |
| CN107236784A (en) | Staphylococcus aureus standard sample and preparation method thereof in milk powder | |
| KR20110011598A (en) | Method and medium for detecting the presence or absence of staphylococcus aureus in a test sample | |
| CN107227336A (en) | Coliform standard sample and preparation method thereof in food | |
| CN109762772A (en) | Pseudomonas aeruginosa qualitative criteria sample and preparation method in water soluble cosmetics | |
| CN107043803A (en) | Yeast and mold sum numerical ability verification sample and preparation method thereof in medicine | |
| CN109762771A (en) | Staphylococcus aureus qualitative criteria sample and preparation method in water soluble cosmetics | |
| CN109762870A (en) | Heat-resisting coliform qualitative criteria sample and preparation method in water soluble cosmetics | |
| CN109797191A (en) | Total plate count standard sample and preparation method in water soluble cosmetics | |
| CN107227338A (en) | ETEC proficiency testing sample and preparation method thereof in Drinking Water | |
| CN109971673A (en) | Clostridium sporogenes employment and suitability test (E & ST) bacterial strain and preparation method thereof | |
| CN109797108A (en) | Aspergillus niger employment and suitability test (E & ST) bacterial strain and preparation method thereof | |
| CN107236783A (en) | Salmonella standard sample and preparation method thereof in milk powder | |
| CN107090489A (en) | Total plate count standard sample and preparation method thereof in milk powder | |
| CN109880880A (en) | Moulds and yeasts count standard sample and preparation method in water soluble cosmetics | |
| CN107142302A (en) | Coliform standard sample and preparation method thereof in milk powder | |
| CN107190045A (en) | Total coli group proficiency testing sample and preparation method thereof in Drinking Water | |
| CN109735469A (en) | Escherichia coli employment and suitability test (E & ST) bacterial strain and preparation method thereof | |
| CN109971672A (en) | Pseudomonas aeruginosa employment and suitability test (E & ST) bacterial strain and preparation method thereof | |
| Thomas et al. | Significant effects due to peptone in Kelman medium on colony characteristics and virulence of Ralstonia solanacearum in tomato | |
| Garcia-Armesto et al. | Modern microbiological methods for foods: colony count and direct count methods. A review | |
| CN109735459B (en) | Candida albicans applicability test strain and preparation method thereof | |
| CN107058459A (en) | Total plate count proficiency testing sample and preparation method thereof in Drinking Water |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190517 |
|
| RJ01 | Rejection of invention patent application after publication |