CN109757723A - A kind of eyesight protection function food and preparation method thereof containing fucoxanthol - Google Patents
A kind of eyesight protection function food and preparation method thereof containing fucoxanthol Download PDFInfo
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Abstract
The invention belongs to field of food, disclose a kind of eyesight protection function food and preparation method thereof containing fucoxanthol.The eyesight protection function food containing fucoxanthol contains fucoxanthol -5~20 parts by weight of protein nano particle, 10~30 parts by weight of oyster peptide, degradation 0.1~1 parts by weight of sulfated polysaccharide, remaining is water and/or solid filler auxiliary material.Eyesight protection function food provided by the invention containing fucoxanthol is using fucoxanthol-protein nano particle as core functional component, by albumen assemble nanometer technology, realizes fucoxanthol in the high-efficiency delivery of organum visuale, product greatly improved the effect of property；The stability and water solubility for improving fucoxanthol simultaneously, enable product to keep its bioactivity for a long time.In addition, fucoxanthol-protein nano particle and oyster peptide are carried out compatible use in the eyesight protection function food provided by the invention containing fucoxanthol, the absorption efficiency of fucoxanthol can be further improved.
Description
Technical field
The invention belongs to field of food, a kind of eyesight protection function food containing fucoxanthol and its preparation are specifically disclosed
Method.
Background technique
Eyes are human physiological metabolism's organs the most active, due to the needs that vision generates, eyes are caused (especially to regard
Nethike embrane) it is in the environment of high oxygen pressure, high polyunsaturated fatty acid, Gao Juguang for a long time, it is highly prone to oxidativestress damage.With this
Simultaneously as the needs of the modern life and working method, the video frequency terminal apparatus such as wideband mobile phone, computer, TV are very general
And it is exposed to light radiation environment for a long time and has been further exacerbated by the stress damage for using asthenopia and organum visuale.As children, blueness are few
The disease incidence of the ophthalmology diseases such as the rate of myopia and cataract, treating senile maculopathy in year is constantly soaring, depending on health problem
As public's focus of attention.Therefore, the angle supplemented from diet nutritional, improves the oxidation resistance of organum visuale, is to answer
To the effective means for working as forward sight health problem.
Fucoxanthin is the secondary metabolite that brown alga adapts to the extreme luminous environment in ocean, and unique allene structure imparts
Its high anti-oxidation activity (13.5 times of vitamin E), and have proven to have the function of good inhibition retinal light damage (Liu
etal.,2016).For example, patent application " new application that fucoxanthin is used for sight protectio " (CN105878230A), a kind of " shield
The functional food of liver improving eyesight " (CN105996027A) is disclosed to be eaten by the eyesight protection function of function factor of fucoxanthin
Product.However, being found by zoopery, continuous gavage mouse fucoxanthin only detected rock algae after 2 weeks in organum visuale
Flavol.It can be seen that really play eyesight protection function is fucoxanthol, and not fucoxanthin itself.Therefore, it is necessary to
Fucoxanthin is changed into fucoxanthol by modern biotechnology, and is applied in sight protectio Related product.However,
It not yet appears in the newspapers by the Related product of core functional component of fucoxanthol at present.At the same time, fucoxanthol is improved regarding
The targeted delivery efficiency of organ is to develop another key technology bottleneck of sight protectio Related product, needs to be broken through.
Summary of the invention
In view of the deficiencies of the prior art, it is an object of the present invention to design by product formula, a kind of composition is provided
Reasonable composition, safety is without side-effects, can effectively improve the eyesight protection function food containing fucoxanthol of organum visuale's oxidation resistance
Product.
Another mesh of the invention is to provide a kind of production of organum visuale's targeting conevying efficiency that can be improved fucoxanthol
Technique, to meet requirement of the eyesight protection function food development to low cost, suitable large-scale production.
Specifically, the present invention provides a kind of eyesight protection function food containing fucoxanthol, wherein the kukersite algae is yellow
The eyesight protection function food of alcohol contain fucoxanthol -5~20 parts by weight of protein nano particle, 10~30 parts by weight of oyster peptide,
Degradation 0.1~1 parts by weight of sulfated polysaccharide, remaining is water and/or solid filler auxiliary material.
Preferably, in the fucoxanthol-protein nano particle the weight ratio of fucoxanthol and carrier protein be 1:(8~
12)。
Preferably, carrier protein contained in the fucoxanthol-protein nano particle is selected from bovine serum albumin(BSA), whey
At least one of albumen, zeins, casein and soybean protein.
Preferably, the fucoxanthol-protein nano particle average grain diameter is 50~500nm.
Preferably, the molecular weight of the oyster peptide is≤5000Da.The oyster peptide refers to using oyster meat as raw material, passes through
Protease hydrolytic, isolate and purify based on small active peptides and rich in a variety of function such as taurine, arginine and zinc selenium
Imitate the hydrolysate of ingredient.
Preferably, the molecular weight of the degradation sulfated polysaccharide is≤4000Da.The degradation sulfated polysaccharide, which refers to, to be derived from
Sulfated polysaccharide in seaweed or marine animal is through biology, sulfate group content >=20wt% that physically or chemically method hydrolyzes
Polysaccharide.
Preferably, the eyesight protection function food containing fucoxanthol is with capsule, tablet, electuary, oral solution or beverage
Form use.
Term " solid filler auxiliary material " includes common some additives and shaping additive in food.In addition, water and/
Or the dosage of solid filler auxiliary material should depend on the circumstances, and described will contain fucoxanthol-protein nano particle, oyster peptide and drop
Solution sulfated polysaccharide is configured to the use in the form of capsule, tablet, electuary, oral solution or beverage, to this those skilled in the art
Member can know that therefore not to repeat here.
The preparation method of the present invention also provides the described eyesight protection function food containing fucoxanthol, wherein this method
The following steps are included:
(1) prepared by fucoxanthol: fucoxanthin being dissolved in the buffer containing hydrophilic emulsifier, cholesteryl ester is added
Enzyme mixes, is filled with nitrogen, and 5~20h is reacted in the case where being protected from light 30~40 DEG C of environment, is obtained after concentrated, dry rich in fucoxanthol
Mixture；
(2) fucoxanthol-protein nano particle preparation: compound concentration is the carrier protein aqueous solution of 0.1~1wt%, and
Its pH value is adjusted to 10~12, fatty acid is then added and stirs evenly simultaneously ultrasonic disperse, obtains carrier protein solution；It will be described
Mixture and the carrier protein solution rich in fucoxanthol are stirred to react 10~60 minutes, and gained is stirred to react product
PH value recall to 6~8, fucoxanthol-protein nano particle is obtained after spray drying；
(3) by the fucoxanthol-protein nano particle, oyster peptide, degradation sulfated polysaccharide, water and/or solid-filling agent
Auxiliary material mixes in proportion, later by gained liquid mixture homogeneous, filtering, or obtained solid mixture is sieved, is contained
The eyesight protection function food of fucoxanthol.
Preferably, in step (1), the hydrophilic emulsifier is selected from soybean lecithin, lecithin, bile salt, tween, sugarcane
At least one of sugar fatty acid ester and taurine.
Preferably, in step (1), the concentration of hydrophilic emulsifier is 0.1 in the buffer containing hydrophilic emulsifier
~2wt%
Preferably, in step (1), the pH value of the buffer is 6~8.
Preferably, in step (1), the mass ratio of the cholesterol esterase and fucoxanthin is 1:(100~1000).
Preferably, in step (2), the weight ratio of the fatty acid and carrier protein is 1:(18~22).
Preferably, in step (2), in terms of fucoxanthol described in rich in fucoxanthol mixture in terms of carrier protein
The carrier protein solution weight ratio be 1:(8~12).Wherein, by the mixture rich in fucoxanthol and the load
Body protein solution is stirred to react can be added institute for the mixture rich in fucoxanthin is used a small amount of solvatrope later
It states in carrier protein solution, is stirred to react 10~60 minutes, be preferably stirred to react 20~40 minutes.
Preferably, in step (2), the fatty acid is selected from oleic acid, linoleic acid, palmitinic acid, arachidonic acid and 22
At least one of carbon acid.
Preferably, in step (2), it is tart flavour that the pH value for being stirred to react product, which is recalled to reagent used by 6~8,
Agent, the acid are selected from least one of acetic acid, citric acid, lactic acid, tartaric acid, malic acid and glucolactone.
The present inventor has found that Zn-ef ficiency can promote carotenoid nutriment after further investigation
It absorbs, improves absorption efficiency.Therefore, the present invention carries out compatibility by fucoxanthol and the oyster peptide rich in organic Zinc, in this way
It can be improved the absorption and targeted delivery efficiency of fucoxanthol.It is mentioned in addition, the present invention is used as by albumen self-assembled nanometer technology
The effective means of high fucoxanthol absorption efficiency and stability further improves sight protectio effect of product.
Compared with prior art, the invention has the following advantages that
(1) the eyesight protection function food provided by the invention containing fucoxanthol is with fucoxanthol-protein nano particle
Core functional component.Fucoxanthol is water-soluble substances, there are stability it is poor, absorption efficiency is low the problems such as.The present invention passes through egg
White assemble nanometer technology realizes fucoxanthol in the high-efficiency delivery of organum visuale, product greatly improved the effect of property；It mentions simultaneously
The high stability of fucoxanthol and water-soluble, enables product to keep its bioactivity for a long time.Further, since rock algae is yellow
Alcohol-protein nano particle has good water solubility, improves the fitness of process of fucoxanthol, does not need high-pressure homogeneous Deng units
Operation, simplifies production technology, reduces production cost.
(2) by fucoxanthol-protein nano particle in the eyesight protection function food provided by the invention containing fucoxanthol
Compatible use is carried out with oyster peptide, can be further improved the absorption efficiency of fucoxanthol.At the same time, Zn-ef ficiency or organum visuale
Necessary microelement is the important cofactors of anti-oxidant protease etc., carries out compatibility with fucoxanthol, can further mention
High product protects the effect of eyesight.
(3) the necessary component in the eyesight protection function food provided by the invention containing fucoxanthol --- degradation sulfuric acid is more
Sugar is derived from the natural polysaccharide of seaweed or marine animal, has good bacteriostasis, can effectively ensure that the health peace of product
Entirely, without adding any preservative.Therefore, product has advantage that is safe and non-toxic, being suitable for any crowd.
(4) modern food production technology is used, stable product quality, simple production process is low in cost, is suitble to scale
Industrial production.
Detailed description of the invention
Fig. 1 is the preparation technology flow chart of the eyesight protection function food provided by the invention containing fucoxanthol；
Fig. 2 is the fucoxanthol liquid phase detection figure in naive mice eyeball；
Fig. 3 is the fucoxanthol liquid phase detection figure in embodiment 1 (fucoxanthol-protein nano particle group) eyeball of mouse；
Fig. 4 be blank group, in 1 formula group eyeball of mouse of embodiment total antioxidant capacity comparison diagram.
Specific embodiment
The embodiment of the present invention is described below in detail, the examples of the embodiments are intended to be used to explain the present invention, and cannot
It is interpreted as limitation of the present invention.In the examples where no specific technique or condition is specified, described according to the literature in the art
Technology or conditions or carried out according to product description.Reagents or instruments used without specified manufacturer is that can lead to
Cross the conventional products of commercially available acquisition.
Embodiment 1
The eyesight protection function food containing fucoxanthol is prepared according to process flow as shown in Figure 1, is specifically included following
Step:
(1) prepared by fucoxanthol: fucoxanthin is dissolved in the phosphate buffer for the soybean lecithin containing 2wt% that pH is 7.0,
Cholesterol esterase (mass ratio of cholesterol esterase and fucoxanthin is 1:1000) is added, mixes, is filled with nitrogen, is being protected from light 37 DEG C
React 12h under environment, be concentrated under reduced pressure, be dried in vacuo after obtain the dry powder rich in fucoxanthol.
(2) fucoxanthol-protein nano particle preparation: compound concentration is the Bovine Serum Albumin in Aqueous Solution of 0.5wt%, and
PH value is adjusted to 11 with sodium hydroxide, and oleic acid (mass ratio of oleic acid and bovine serum albumin(BSA) is 1:18) is then added and stirs evenly
And ultrasound 30 minutes, obtain carrier protein solution；It will be enriched in the dry powder (matter of fucoxanthol and bovine serum albumin(BSA) of fucoxanthol
Amount is than being added in carrier protein solution, reacting 30 minutes under stirring condition, use vinegar after for a small amount of alkaline ethanol hydrotropy of 1:8)
Acid recalls to the pH value of mixed solution to 7.0 (self assemblies), and spray drying obtains fucoxanthol-bovine serum albumin(BSA) nanometer
Grain.It is analyzed through Malvern nano particle size instrument, the average grain diameter of the nano particle is 50nm.
(3) with fucoxanthol -5 parts by weight of bovine serum albumin(BSA) nano particle, oyster peptide (molecular weight is≤5000Da) 10
The ratio of parts by weight, degradation kelp polysaccharide sulfate (molecular weight is≤4000Da, and sulfate group content is 28wt%) 0.1 parts by weight
Above-mentioned each component is weighed, appropriate magnesium stearate and starch are added in prescription, mixes, is filled after sieving with Autocapsulefillingmachine
Enter hard capsule, every loading amount 0.5g, sterilizing is packed to get capsule class functional food.
Embodiment 2
The eyesight protection function food containing fucoxanthol is prepared according to process flow as shown in Figure 1, is specifically included following
Step:
(1) prepared by fucoxanthol: fucoxanthin is dissolved in the phosphate buffer for the 0.5wt% bile salt that pH is 6.0,
Cholesterol esterase (mass ratio of cholesterol esterase and fucoxanthin is 1:1000) is added, mixes, is filled with nitrogen, is being protected from light 37 DEG C
React 12h under environment, be concentrated under reduced pressure, be dried in vacuo after obtain the dry powder rich in fucoxanthol.
(2) fucoxanthol-protein nano particle preparation: compound concentration is the aqueous whey protein solution of 0.5wt%, and uses hydrogen
Sodium oxide molybdena adjusts pH value to 11, and linoleic acid (mass ratio of linoleic acid and lactalbumin is 1:22) is then added and stirs evenly, and
Ultrasound 30 minutes, obtains carrier protein solution；Will be enriched in the dry powder of fucoxanthol, (mass ratio of fucoxanthol and lactalbumin is
It 1:10) with after a small amount of alkaline ethanol hydrotropy, is added in carrier protein solution, is reacted 30 minutes under stirring condition, use citric acid
The pH value of mixed solution is recalled to 7.0 (self assemblies), spray drying obtains fucoxanthol-lactalbumin nano particle.Through horse
You analyze literary nano particle size instrument, and the average grain diameter of the nano particle is 245nm.
(3) with fucoxanthol -12.5 parts by weight of lactalbumin nano particle, oyster peptide (molecular weight is≤5000Da) 20 weights
Measure the ratio of part, degradation Eucheuma sulfated polysaccharide (molecular weight is≤4000Da, and sulfate group content is 32wt%) 0.55 parts by weight
Example weighs above-mentioned each component, and one or more of suitable dextrin, starch, microcrystalline cellulose and lactose is then added as auxiliary
Material is uniformly mixed, and with appropriate 70% (v/v) ethanol wet, softwood processed crosses the granulation of 30 meshes, in 70-80 DEG C of drying, with 60 mesh
Whole grain is sieved, tabletting obtains tablet, every 0.2g, and sterilizing is packed to get tablet class functional food.
Embodiment 3
The eyesight protection function food containing fucoxanthol is prepared according to process flow as shown in Figure 1, is specifically included following
Step:
(1) prepared by fucoxanthol: fucoxanthin being dissolved in the phosphate buffer for the 0.1wt% tween that pH is 8.0, is added
Cholesterol esterase (mass ratio of cholesterol esterase and fucoxanthin is 1:1000), mixes, is filled with nitrogen, is being protected from light 37 DEG C of environment
After lower reaction 12h, be concentrated under reduced pressure, be dried in vacuo after obtain the dry powder rich in fucoxanthol.
(2) fucoxanthol-protein nano particle preparation: compound concentration is the corn alcohol soup-dissolving protein solution of 0.5wt%, and
PH value is adjusted to 11 with sodium hydroxide, and palmitinic acid (mass ratio of palmitinic acid and zeins is 1:20) stirring is then added
Uniformly, and it is 30 minutes ultrasonic, obtain carrier protein solution；It will be enriched in dry powder (fucoxanthol and the molten egg of corn alcohol of fucoxanthol
White mass ratio is 1:10) with after a small amount of alkaline ethanol hydrotropy, it is added in carrier protein solution, 30 points is reacted under stirring condition
Clock is recalled to the pH value of mixed solution to 7.0 (self assemblies) with glucolactone, and spray drying obtains fucoxanthol-corn
Alcohol soluble protein nano particle.It is analyzed through Malvern nano particle size instrument, the average grain diameter of the nano particle is 500nm.
(3) with fucoxanthol -20 parts by weight of zeins nano particle, oyster peptide (molecular weight is≤5000Da) 30
Parts by weight, the ratio of degradation sea cucumber sulfated polysaccharide (molecular weight is≤4000Da, and sulfate group content is 20wt%) 1 parts by weight claim
Above-mentioned each component is taken, it is one of or several to be then added appropriate soluble starch, dextrin, sucrose, lactose and mannitol, with suitable
70% (v/v) ethanol wet is measured, softwood processed crosses the granulation of 20 meshes, and 70-80 DEG C of drying, 60 mesh whole grains, sterilizing, packaging are to get punching
Agent class functional food.
Embodiment 4
The eyesight protection function food containing fucoxanthol is prepared according to process flow as shown in Figure 1, is specifically included following
Step:
(1) prepared by fucoxanthol: fucoxanthin is dissolved in the phosphate buffer for the 1wt% sucrose fatty ester that pH is 7.0
In, cholesterol esterase (mass ratio of cholesterol esterase and fucoxanthin is 1:1000) is added, mixes, is filled with nitrogen, is being protected from light
React 12h under 37 DEG C of environment, be concentrated under reduced pressure, be dried in vacuo after obtain the dry powder rich in fucoxanthol.
(2) fucoxanthol-protein nano particle preparation: compound concentration is the soybean protein aqueous solution of 0.5wt%, and uses hydrogen
Sodium oxide molybdena adjusts pH value to 11, and docosahexaenoic acid is then added, and (mass ratio of docosahexaenoic acid and soybean protein is
It 1:20) stirs evenly and 30 minutes ultrasonic, obtains carrier protein solution；It will be enriched in the dry powder (fucoxanthol and big of fucoxanthol
The mass ratio of legumin is 1:12) with after a small amount of alkaline ethanol hydrotropy, it is added in carrier protein solution, is reacted under stirring condition
30 minutes, the pH value of mixed solution is recalled to 7.0 (self assemblies) with malic acid, spray drying obtains fucoxanthol-soybean egg
White nano particle.It is analyzed through Malvern nano particle size instrument, the average grain diameter of the nano particle is 378nm.
(3) with fucoxanthol -5 parts by weight of soybean protein nano particle, oyster peptide (molecular weight is≤5000Da) 15 weight
(molecular weight is≤4000Da, and sulfate group content claims for the ratio of 0.75 parts by weight of 23wt%) for part, degradation fish-bone sulfated polysaccharide
Above-mentioned each component is taken, is then dissolved in water, suitable sweetener and stabilizer is added, is mixed, homogeneous, is filtered, is sterilized, packaging,
Up to beverage class functional food.
Comparative example 1
Capsule class functional food is prepared according to the method for embodiment 1, unlike, it does not include step (1) and by step (2)
The dry powder rich in fucoxanthol using identical weight part fucoxanthin substitute, obtain reference capsule class functional food.
Comparative example 2
Capsule class functional food is prepared according to the method for embodiment 1, unlike, in step (3), oyster peptide is used into phase
Degradation kelp polysaccharide sulfate with parts by weight substitutes, and obtains reference capsule class functional food.
Functional evaluation test:
(1) evaluation is transported in targeting of the fucoxanthol in organum visuale
Given the test agent: fucoxanthol obtained in the embodiment of the present invention 1-bovine serum albumin(BSA) nano particle.
Sample treatment: 1 gained fucoxanthol of Example-bovine serum albumin(BSA) nano particle is molten by mass volume ratio 1:10
Solution is in deionized water, as test liquid.
Experimental animal: SPF grades of Female ICR mices, 15-20g are purchased from Shanghai Slac Experimental Animal Co., Ltd..
Mouse basal feed ingredient: carbohydrate 64%, protein 21%, fat 4%, fiber 5%, moisture 6%.Above-mentioned feed is equal
It is purchased from Shanghai Slac Experimental Animal Co., Ltd..Raising room temperature: 23 ± 2 DEG C, relative humidity 55 ± 5%.
Animal packet and processing method: after mouse adaptable fed 1 week, being randomly divided into 2 groups according to weight, and every group 10,
Respectively blank group (stomach-filling deionized water), processing group (stomach-filling test liquid).The each 200 μ L test liquid of stomach-filling of every mouse is gone
Ionized water after continuous gavage 2 weeks, is won eyeball of mouse, is ground after liquid nitrogen frozen 1 time a day, and is 1:1's with volume ratio
Ethyl acetate-light petrol mixed solution extracts 3 times, and combined extract takes supernatant after centrifugation, is concentrated into 50 μ L with the nitrogen method of blowing,
With the changes of contents of high performance liquid chromatography (HPLC) analysis concentrate fucoxanthol.
Experimental result:
The chromatogram of HPLC analysis margin group and the fucoxanthol in processing group eyeball of mouse is shown in Fig. 2 and Fig. 3 respectively.From figure
2 and Fig. 3 can be seen that the quantitative analysis regression equation that fucoxanthol is obtained according to fucoxanthol concentration and its HPLC peak area,
The peak area of combination processing group, the fucoxanthol concentration being calculated in concentrate have reached 379.6pmol/mL.Illustrate with rock
Algae flavol-protein nano particle is that the core function ingredient of eyesight protection function food can effectively realize fucoxanthol in organum visuale
Targeted delivery, thus achieve the effect that protect eyesight.
(2) organum visuale's total antioxidant capacity is evaluated
Given the test agent: formulation product obtained in embodiment 1- embodiment 4 and comparative example 1- comparative example 2.
Sample treatment: formulation product is directly dissolved in deionized water by mass volume ratio 1:10, as test liquid.
Experimental animal: SPF grades of Female ICR mices, 15-20g are purchased from Shanghai Slac Experimental Animal Co., Ltd..
Mouse basal feed ingredient: carbohydrate 64%, protein 21%, fat 4%, fiber 5%, moisture 6%.Above-mentioned feed is equal
It is purchased from Shanghai Slac Experimental Animal Co., Ltd..Raising room temperature: 23 ± 2 DEG C, relative humidity 55 ± 5%.
Animal packet and processing method: after mouse adaptable fed 1 week, being randomly divided into 7 groups according to weight, and every group 10,
Respectively blank group (stomach-filling deionized water) and processing group (stomach-filling test liquid).The each 200 μ L test liquid of stomach-filling of every mouse or
Deionized water after continuous gavage 2 weeks, wins eyeball of mouse 1 time a day, with anti-oxidant kit assay organum visuale total antioxidation
The variation of ability.
Experimental result:
The result shows that by continuous stomach-filling in 2 weeks, 2 products therefrom of embodiment 1- embodiment 4 and comparative example 1- comparative example
Total antioxidant capacity has been respectively increased 59.6% ± 2.1%, 62.3% ± 1.9%, 61.8% ± 3.7%, 64.8% ±
2.7%, 38.3% ± 1.1%, 41.1% ± 1.9%.Wherein, eyeball of mouse always resists in 1 products obtained therefrom of blank group and embodiment
Oxidability result is shown in Fig. 4.From fig. 4, it can be seen that by continuous stomach-filling in 2 weeks, the total antioxidation energy of mouse eye in blank group
Power is 9.42U/mL, and the total antioxidant capacity of mouse eye is 15.03U/mL in 1 products obtained therefrom group of embodiment, is mentioned than blank group
It is high by 59.6% ± 2.1%.It is possible thereby to illustrate, eyesight protection function food formula provided by the present invention can effectively improve view
The anti-oxidation stress ability of organ reduces the occurrence risk that myopia occurs and reduces retinal disease.
The preferred embodiment of the present invention has been described above in detail, still, during present invention is not limited to the embodiments described above
Detail within the scope of the technical concept of the present invention can be with various simple variants of the technical solution of the present invention are made, this
A little simple variants all belong to the scope of protection of the present invention.
It is further to note that specific technical features described in the above specific embodiments, in not lance
In the case where shield, it can be combined in any appropriate way.In order to avoid unnecessary repetition, the present invention to it is various can
No further explanation will be given for the combination of energy.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally
The thought of invention, it should also be regarded as the disclosure of the present invention.
Claims (10)
1. a kind of eyesight protection function food containing fucoxanthol, which is characterized in that the sight protectio function containing fucoxanthol
Energy food contains fucoxanthol -5~20 parts by weight of protein nano particle, 10~30 parts by weight of oyster peptide, degradation sulfated polysaccharide 0.1
~1 parts by weight, remaining is water and/or solid filler auxiliary material.
2. the eyesight protection function food according to claim 1 containing fucoxanthol, which is characterized in that the rock algae is yellow
The weight ratio of fucoxanthol and carrier protein is 1:(8~12 in alcohol-protein nano particle).
3. the eyesight protection function food according to claim 1 containing fucoxanthol, which is characterized in that the rock algae is yellow
Carrier protein contained in alcohol-protein nano particle is selected from bovine serum albumin(BSA), lactalbumin, zeins, casein
At least one of with soybean protein.
4. the eyesight protection function food according to claim 1 containing fucoxanthol, which is characterized in that the rock algae is yellow
Alcohol-protein nano particle average grain diameter is 50~500nm.
5. the eyesight protection function food described in any one of -4 containing fucoxanthol according to claim 1, which is characterized in that
The molecular weight of the oyster peptide is≤5000Da.
6. the eyesight protection function food described in any one of -4 containing fucoxanthol according to claim 1, which is characterized in that
The molecular weight of the degradation sulfated polysaccharide is≤4000Da；Content >=20wt% of sulfate group in the degradation sulfated polysaccharide.
7. the eyesight protection function food described in any one of -4 containing fucoxanthol according to claim 1, which is characterized in that
The eyesight protection function food containing fucoxanthol is used in the form of capsule, tablet, electuary, oral solution or beverage.
8. the preparation method of the eyesight protection function food described in any one of claim 1-7 containing fucoxanthol, special
Sign is, method includes the following steps:
(1) prepared by fucoxanthol: fucoxanthin being dissolved in the buffer containing hydrophilic emulsifier, cholesterol esterase is added, is mixed
It is even, it is filled with nitrogen, 5~20h is reacted in the case where being protected from light 30~40 DEG C of environment, is obtained after concentrated, dry mixed rich in fucoxanthol
Close object；
(2) fucoxanthol-protein nano particle preparation: compound concentration be 0.1~1wt% carrier protein aqueous solution, and by its
PH value is adjusted to 10~12, and fatty acid is then added and stirs evenly simultaneously ultrasonic disperse, obtains carrier protein solution；It is rich in by described in
The mixture of fucoxanthol and the carrier protein solution are stirred to react 10~60 minutes, and gained is stirred to react to the pH of product
Value is recalled to 6~8, and fucoxanthol-protein nano particle is obtained after spray drying；
(3) by the fucoxanthol-protein nano particle, oyster peptide, degradation sulfated polysaccharide, water and/or solid-filling agent auxiliary material
It mixes in proportion, later by gained liquid mixture homogeneous, filtering, or obtained solid mixture is sieved, obtains kukersite algae
The eyesight protection function food of flavol.
9. the preparation method of the eyesight protection function food according to claim 8 containing fucoxanthol, which is characterized in that step
Suddenly in (1), the hydrophilic emulsifier is selected from soybean lecithin, lecithin, bile salt, tween, sucrose fatty ester and ox sulphur
At least one of acid；The concentration of hydrophilic emulsifier is 0.1~2wt% in the buffer containing hydrophilic emulsifier；Institute
The pH value for stating buffer is 6~8, and the mass ratio of the cholesterol esterase and fucoxanthin is 1:(100~1000).
10. the preparation method of the eyesight protection function food according to claim 8 containing fucoxanthol, which is characterized in that
In step (2), the weight ratio of the fatty acid and carrier protein is 1:(18~22)；Rock algae is rich in described in terms of fucoxanthol
The mixture of flavol and the weight ratio of the carrier protein solution in terms of carrier protein are 1:(8~12)；
The fatty acid is selected from least one of oleic acid, linoleic acid, palmitinic acid, arachidonic acid and docosahexaenoic acid；
It is acid that the pH value for being stirred to react product, which is recalled to reagent used by 6~8, and the acid is selected from vinegar
At least one of acid, citric acid, lactic acid, tartaric acid, malic acid and glucolactone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910074097.3A CN109757723B (en) | 2019-01-25 | 2019-01-25 | A food containing fucoxanthin for protecting eyesight and its preparation method |
Applications Claiming Priority (1)
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101415395A (en) * | 2006-03-29 | 2009-04-22 | 富士胶片株式会社 | Casein nanoparticle |
| CN104789611A (en) * | 2015-03-31 | 2015-07-22 | 国家海洋局第三海洋研究所 | Preparation method of fucoxanthol |
| CN105996027A (en) * | 2016-05-07 | 2016-10-12 | 集美大学 | Functional food with effects of protecting liver and improving eyesight |
| CN107041874A (en) * | 2016-11-29 | 2017-08-15 | 宁波大学 | A kind of fucoxanthin microcapsules and its preparation technology |
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Patent Citations (4)
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| CN101415395A (en) * | 2006-03-29 | 2009-04-22 | 富士胶片株式会社 | Casein nanoparticle |
| CN104789611A (en) * | 2015-03-31 | 2015-07-22 | 国家海洋局第三海洋研究所 | Preparation method of fucoxanthol |
| CN105996027A (en) * | 2016-05-07 | 2016-10-12 | 集美大学 | Functional food with effects of protecting liver and improving eyesight |
| CN107041874A (en) * | 2016-11-29 | 2017-08-15 | 宁波大学 | A kind of fucoxanthin microcapsules and its preparation technology |
Non-Patent Citations (1)
| Title |
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| HAO LI: "Stability, bioactivity, and bioaccessibility of fucoxanthin in zein-caseinate composite nanoparticles fabricated at neutral pH by antisolvent precipitation", 《FOOD HYDROCOLLOIDS》 * |
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