CN109752831A - A kind of microscope auto focusing method, system and device that focal time is controllable - Google Patents
A kind of microscope auto focusing method, system and device that focal time is controllable Download PDFInfo
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Abstract
The invention discloses a kind of microscope auto focusing method, system and devices that focal time is controllable, this method comprises: control article carrying platform is moved to a focusing surface position；By the image of control focal time control acquisition different location, the image information of acquisition is received, and calculate its gradient value；Greatest gradient value corresponding position is exported, and saves as present convergence face position.The present invention solves the problems, such as that microscope autofocus system focal time is uncontrollable and focuses easy local extremum, and method is simply easily implemented.
Description
Technical field
The invention belongs to the technical fields of microscope control system, more particularly, to a kind of microscope that focal time is controllable
Auto focusing method, system and device.
Background technique
Microscope is research one of modern medicine and the main tool of life science, with the development of the relevant technologies, people
Increasingly higher demands are proposed to microscopical the degree of automation.Techniques of Automatic Focusing is the core in microscope control system
Part, therefore how to effectively realize microscopical automatic focusing and surveyed in high precision as Microtomographic Analysis System performance, realization is improved
The critical issue of amount.
Microscope focus method is mainly segmented into two major classes at present: one kind is confocal laser method, it is to utilize laser beam
Form point light source through illumination pin hole, each spot scan to focal plane in sample, the illuminated point on sample, at exploration hole at
Picture is received by the photomultiplier tube (PMT) after exploration hole, forms fluorescent image on the computer screen rapidly, however this method
Complexity is high, is not easy to realize；Another kind of method is auto-focusing algorithm, passes through the acutance of focal plane image and non-focal plane image, side
The features such as edge realize automatic focusing, such as use absolute variance function, plane differential sum of squares function, shade of gray operator letter
The evaluation functions such as number, gray scale difference point-score, energy spectrum function, wavelet transformation, Luo Baici operator, Laplace operator, this method pair
Hardware process speed requires height, therefore further development has certain limitation to it.
With the raising of Computing ability and the change of consumption structure and the hair of various image processing techniques
Exhibition, machine vision technique are more and more applied to the various aspects of production and living.Existing microscope Techniques of Automatic Focusing is usual
It is to carry out analysis picture quality superiority and inferiority using computer to automatically select proper picture to realize that microscope focuses automatically.But it is existing
Microscope Techniques of Automatic Focusing exist have the following disadvantages:
(1) existing auto-focusing algorithm, such as hill-climbing algorithm, focal time not can determine that, be unable to control, and influence entire
The progress of scanning；
(2) it is easy to appear the case where local extremum focuses in automatic focusing, influences automatic focusing accuracy；
(3) due to the limitation of technology, the flatness of microscope slide platform is inadequate, and auto-focusing algorithm effect is caused to pay no attention to
Think.
In conclusion how further effectively to realize that microscope focuses automatically for the prior art, when how to determine focusing
Between and avoid the occurrence of local extremum and focus the problem of influencing the automatic focusing efficiency of microscope and effect, still shortage effective solution
Scheme.
Summary of the invention
For the deficiencies in the prior art, solve how further effectively to realize that microscope gathers automatically in the prior art
Coke, how determining focal time and avoiding the occurrence of local extremum and focus influences asking for the automatic focusing efficiency of microscope and effect
Topic, the present invention provides a kind of microscope auto focusing method, system and devices that focal time is controllable, microscopical automatic
It effectively realizes that focal time is controllable, reduces the waste of focal time in focusing, while avoiding the occurrence of the case where local extremum focuses,
Scan efficiency is improved, the balance of microscope focal time and focusing accuracy is reached.
The first object of the present invention is to provide a kind of microscope auto focusing method that focal time is controllable.
To achieve the goals above, the present invention is using a kind of following technical solution:
A kind of microscope auto focusing method that focal time is controllable, this method comprises:
Control article carrying platform is moved to a focusing surface position；
By the image of control focal time control acquisition different location, the image information of acquisition is received, and calculate its ladder
Angle value；
Greatest gradient value corresponding position is exported, and saves as present convergence face position.
Scheme as a further preference, the image by control focal time control acquisition different location use
Acquire the image of the focusing surface nearby fixed quantity at equal intervals.
In the present invention, the initial position that upper focusing surface position focuses automatically as microscope is chosen, is effectively reduced certainly
Dynamic focal time, the distance between the quantity of fixed acquisition image and fixed acquisition image, effectively control focal time, focuses
Effect has clear improvement, the gradient value Chooser greatest gradient value corresponding position conduct of different location image near comparison focusing surface
New focusing surface position, avoids the occurrence of the case where local extremum is focused.
Scheme as a further preference, the interval acquired between image are true according to micro objective amplification factor
It is fixed；The product of spacing distance and fixed number magnitude covers all focus points.
Scheme as a further preference receives the focusing surface of acquisition fixed quantity at equal intervals nearby in the method
Image include:
Control article carrying platform moves down 2 times of spacing distances along Z axis, receives and acquires the location drawing picture；
Control article carrying platform moves up spacing distance along Z axis, receives and acquires the location drawing picture, repeats the step, until
Fixed quantity image is completed in acquisition.
Scheme as a further preference, for 20 times of object lens of microscope, spacing distance selects 20 μm.
Scheme as a further preference, fixed quantity used are 5.
Scheme as a further preference, in the method, before control article carrying platform is moved to a focusing surface position, into
Line focusing pretreatment；
The focusing pre-processes
The manual acquisition focussing plane of acquisition fixed quantity image at equal intervals nearby is received, gradient curve is converted to；
Judgement focuses the uniqueness of gradient curve extreme point in pretreatment, if uniquely, it is corresponding to export gradient value at extreme point
Position, and save as focusing surface position；Otherwise, it is focused automatically.
Scheme as a further preference acquires the interval between image according to microscope in focusing pretreatment
Object lens magnification determines；The product of spacing distance and fixed number magnitude covers all focus points.
Scheme as a further preference, focus pretreatment in, receive acquisition manual acquisition focussing plane nearby etc.
Being spaced fixed quantity image includes:
Control article carrying platform moves down 30 times of spacing distances along Z axis, receives and acquires the location drawing picture；
Control article carrying platform moves up spacing distance along Z axis, receives and acquires the location drawing picture, repeats the step, until
Fixed quantity image is completed in acquisition.
Scheme as a further preference, in focusing pretreatment, for 20 times of object lens of microscope, spacing distance selects 4 μ
M, fixed quantity used are 61.
The second object of the present invention is to provide a kind of computer readable storage medium.
To achieve the goals above, the present invention is using a kind of following technical solution:
A kind of computer readable storage medium, wherein being stored with a plurality of instruction, described instruction is suitable for by terminal device equipment
Processor load and execute following processing:
Control article carrying platform is moved to a focusing surface position；
By the image of control focal time control acquisition different location, the image information of acquisition is received, and calculate its ladder
Angle value；
Greatest gradient value corresponding position is exported, and saves as present convergence face position.
The third object of the present invention is to provide a kind of auto focusing device of microscope that focal time is controllable.
To achieve the goals above, the present invention is using a kind of following technical solution:
A kind of auto focusing device of microscope that focal time is controllable, using computing terminal equipment, including processor and meter
Calculation machine readable storage medium storing program for executing, processor is for realizing each instruction；Computer readable storage medium is described for storing a plurality of instruction
Instruction is suitable for being loaded by processor and executing following processing:
Control article carrying platform is moved to a focusing surface position；
By the image of control focal time control acquisition different location, the image information of acquisition is received, and calculate its ladder
Angle value；
Greatest gradient value corresponding position is exported, and saves as present convergence face position.
The fourth object of the present invention is to provide a kind of microscope autofocus system that focal time is controllable.
To achieve the goals above, the present invention is using a kind of following technical solution:
A kind of microscope autofocus system that focal time is controllable, comprising:
The controllable auto focusing device of microscope of focal time, respectively with image collecting device, article carrying platform driving device
Connection, the article carrying platform driving device are connect with article carrying platform；
The controllable auto focusing device of microscope of the focal time is moved to a focusing surface for controlling article carrying platform
Position；By the image of control focal time control acquisition different location, the image information of acquisition is received, and calculate its gradient
Value；Greatest gradient value corresponding position is exported, and saves as present convergence face position.；
Described image acquisition device is transmitted to described for acquiring the image information that article carrying platform is moved to designated position
The controllable auto focusing device of microscope of focal time；
The article carrying platform driving device is used to receive the finger of the controllable auto focusing device of microscope of the focal time
Enable driving article carrying platform mobile.
Beneficial effects of the present invention:
1, a kind of focal time of the present invention controllable microscope auto focusing method, system and device effectively solves
The problem of microscope autofocus system focal time of having determined is uncontrollable and focuses easy local extremum, and method is simple easily real
It applies；
2, a kind of focal time of the present invention controllable microscope auto focusing method, system and device, in selection
The initial position that one focusing surface position focuses automatically as microscope effectively reduces automatic focal time, fixed acquisition image
The distance between quantity and fixed acquisition image, effectively control focal time, focusing effect have clear improvement, avoid the occurrence of
The case where local extremum is focused；
3, a kind of focal time of the present invention controllable microscope auto focusing method, system and device, automatic
The image of fixed quantity at equal intervals is acquired in focusing, acquires the interval between image according to micro objective times magnification
Number determines；The product of spacing distance and fixed number magnitude covers all focus points, effectively prevents omitting asking for best focal point
Topic；
4, a kind of focal time of the present invention controllable microscope auto focusing method, system and device, for poly-
Coke is easy to appear local extremum problem, using Z axis stroke scanning constant, solves local focal extreme-value problem, improves focusing effect.
Detailed description of the invention
The accompanying drawings constituting a part of this application is used to provide further understanding of the present application, and the application's shows
Meaning property embodiment and its explanation are not constituted an undue limitation on the present application for explaining the application.
Fig. 1 is the method flow diagram in the present invention；
Fig. 2 is the geometrical model schematic diagram in the present invention；
Fig. 3 is seven sections of S-shaped acceleration and deceleration curves schematic diagram in the present invention；
Fig. 4 is the working space subregion schematic diagram in the present invention；
Fig. 5 is the solution of equation physical analysis model schematic of embodiment 2 in the present invention.
Specific embodiment:
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
It is noted that following detailed description is all illustrative, it is intended to provide further instruction to the application.Unless another
It indicates, all technical and scientific terms that the present embodiment uses have and the application person of an ordinary skill in the technical field
Normally understood identical meanings.
It should be noted that term used herein above is merely to describe specific embodiment, and be not intended to restricted root
According to the illustrative embodiments of the application.As used herein, unless the context clearly indicates otherwise, otherwise singular
Also it is intended to include plural form, additionally, it should be understood that, when in the present specification using term "comprising" and/or " packet
Include " when, indicate existing characteristics, step, operation, device, component and/or their combination.
For the deficiencies in the prior art, solve how further effectively to realize that microscope gathers automatically in the prior art
Coke, how determining focal time and avoiding the occurrence of local extremum and focus influences asking for the automatic focusing efficiency of microscope and effect
Topic, the present invention provides a kind of microscope auto focusing method, system and devices that focal time is controllable, microscopical automatic
It effectively realizes that focal time is controllable, reduces the waste of focal time in focusing, while avoiding the occurrence of the case where local extremum focuses,
Scan efficiency is improved, the balance of microscope focal time and focusing accuracy is reached.
In the absence of conflict, the features in the embodiments and the embodiments of the present application can be combined with each other.It ties below
Closing attached drawing, the invention will be further described with embodiment.
Embodiment 1:
The purpose of the present embodiment 1 is to provide a kind of microscope auto focusing method that focal time is controllable.
In the present embodiment, northern high novel microscope has been selected, object lens select 20 times of object lens.
To achieve the goals above, the present invention is using a kind of following technical solution:
As shown in Figure 1,
A kind of microscope auto focusing method that focal time is controllable, this method comprises:
Step (1): control article carrying platform is moved to a focusing surface position；
Step (2): by the image of control focal time control acquisition different location, receiving the image information of acquisition, and
Calculate its gradient value；
Step (3): output greatest gradient value corresponding position, and save as present convergence face position.
It is used described in step (2) by the image of control focal time control acquisition different location and is acquiring the focusing
The image of face nearby fixed quantity at equal intervals.
In the present invention, the initial position that upper focusing surface position focuses automatically as microscope is chosen, is effectively reduced certainly
Dynamic focal time, the distance between the quantity of fixed acquisition image and fixed acquisition image, effectively control focal time, focuses
Effect has clear improvement, and avoids the occurrence of the case where local extremum is focused.
In the present embodiment, to solve the problems, such as local focal, improve the effect of focusing, to high 20 times of the novel microscope in north
The image trace observation of sample chips under object lens, carries out stringent data analysis, focuses automatically in the controllable microscope of focal time
Following focusing pre-treatment step is executed in device:
(a) focussing plane position that article carrying platform position searches out will be manually adjusted as initial position；
(b) to article carrying platform driving device send first instruction, control article carrying platform driving device drive article carrying platform from
The position of focussing plane moves down 120 units, each unit 1um along Z axis；
(c) image information of the position after image acquisition device is mobile, and it is sent to controllable micro- of focal time
Mirror autofocus handles image information in the controllable auto focusing device of microscope of focal time and carries out gradient calculating,
And save gradient value；
(d) the second instruction is sent to article carrying platform driving device, control article carrying platform driving device driving article carrying platform is along Z
Axis moves up 4 units, each unit 1um；
(e) image information of the position after image acquisition device is mobile, and it is sent to controllable micro- of focal time
Mirror autofocus handles image information in the controllable auto focusing device of microscope of focal time and carries out gradient calculating,
And save gradient value；
(f) step (d) and step (e) 60 times are repeated, all gradient values are preserved, draws gradient curve figure；
(g) judgement focuses the uniqueness of gradient curve extreme point in pretreatment, if uniquely, exporting gradient value pair at extreme point
Position is answered, and saves as focusing surface position；Otherwise, it is focused automatically.
After many experiments, wherein two representational curve graphs, such as Fig. 2 are taken, shown in Fig. 3, it can be seen from the figure that
There are multiple extreme points for curve, if necessarily will appear the phenomenon that local extremum focuses using traditional climbing method focusing at this time, lead
It causes to focus failure；From the information in figure can also be seen that gradient value occur significant change range it is relatively fixed, it can be seen that,
Under 20 times of object lens, the position of focusing surface be also it is relatively-stationary, this focus plane and last time focus plane will not deposit
In too big variation, there is small variation that can be modified by corresponding method, using the automatic focusing of the present embodiment
Method is focused automatically, and following focusing pre-treatment step is executed in the controllable auto focusing device of microscope of focal time:
Step (1): third instruction is sent to article carrying platform driving device, control article carrying platform driving device driving loading is flat
Platform is moved to a focusing surface position；
Step (2): the image of the focusing surface of acquisition nearby fixed quantity at equal intervals is received, and calculates its gradient value；
In step (2), the interval acquired between image is determined according to micro objective amplification factor；Spacing distance with
The product of fixed number magnitude covers all focus points.
In step (2), the image for receiving the focusing surface of acquisition nearby fixed quantity at equal intervals includes:
Step (2-1): the 4th instruction is sent to article carrying platform driving device, control article carrying platform driving device drives loading
Platform moves down 40 units, each unit 1um along Z axis from the position of focussing plane；
Step (2-2): the image information of the position after image acquisition device is mobile, and it is controllable to be sent to focal time
Auto focusing device of microscope, in the controllable auto focusing device of microscope of focal time handle image information carry out gradient
It calculates, and saves gradient value；
Step (2-3): the 5th instruction is sent to article carrying platform driving device, control article carrying platform driving device drives loading
Platform moves up 20 units, each unit 1um along Z axis；
Step (2-4): the image information of the position after image acquisition device is mobile, and it is controllable to be sent to focal time
Auto focusing device of microscope, in the controllable auto focusing device of microscope of focal time handle image information carry out gradient
It calculates, and saves gradient value；
Step (2-5): repeating step (2-3) and step (2-4) 3 times, and all gradient values are preserved.Such as Fig. 4 institute
Show.
Step (3): output greatest gradient value corresponding position, and save as focusing surface position.
In the controllable auto focusing device of microscope of focal time, the gradient value of all acquisition images is compared,
Greatest gradient value is selected, focusing surface position is saved as；Gradient value it is maximum for clearest image as this focus as a result, adopting
Collect the position of image as the initial position focused next time.
The greatest gradient value corresponding position is exported, it is poly- that control article carrying platform driving device driving article carrying platform is moved to this
Automatic focus is realized in position of focal plane.
Since the picture number of acquisition is fixed, and before acquisition, focusing surface has had preliminary judgement, so this is poly-
The focal time of burnt system is also relatively fixed, and focusing effect has clear improvement, and does not appear in local extremum and is focused
The case where.
Embodiment 2:
The purpose of the present embodiment 2 is to provide a kind of computer readable storage medium.
To achieve the goals above, the present invention is using a kind of following technical solution:
A kind of computer readable storage medium, wherein being stored with a plurality of instruction, described instruction is suitable for by terminal device equipment
Processor load and execute following processing:
Control article carrying platform is moved to a focusing surface position；
By the image of control focal time control acquisition different location, the image information of acquisition is received, and calculate its ladder
Angle value；
Greatest gradient value corresponding position is exported, and saves as present convergence face position.
In the present embodiment, the example of computer readable recording medium include magnetic storage medium (for example, ROM, RAM, USB,
Floppy disk, hard disk etc.), optical record medium (for example, CD-ROM or DVD), PC interface is (for example, PCI, PCI-Expres, WiFi
Deng) etc..However, various aspects of the disclosure is without being limited thereto.
Embodiment 3:
The purpose of the present embodiment 3 is to provide a kind of auto focusing device of microscope that focal time is controllable.
To achieve the goals above, the present invention is using a kind of following technical solution:
A kind of auto focusing device of microscope that focal time is controllable, using a kind of computing terminal equipment, including processor
And computer readable storage medium, processor is for realizing each instruction；Computer readable storage medium is used to store a plurality of instruction,
Described instruction is suitable for being loaded by processor and executing following processing:
Control article carrying platform is moved to a focusing surface position；
By the image of control focal time control acquisition different location, the image information of acquisition is received, and calculate its ladder
Angle value；
Greatest gradient value corresponding position is exported, and saves as present convergence face position.
It will be understood by those skilled in the art that each module of the above invention or each step can use general computer
Device realizes that optionally, they can be realized with the program code that computing device can perform, it is thus possible to which they are deposited
Storage be performed by computing device in the storage device, perhaps they are fabricated to each integrated circuit modules or by it
In multiple modules or step be fabricated to single integrated circuit module to realize.The present invention is not limited to any specific hardware
With the combination of software.
Embodiment 4:
The purpose of the present embodiment 4 is to provide a kind of microscope autofocus system that focal time is controllable.
To achieve the goals above, the present invention is using a kind of following technical solution:
It is illustrated in figure 5 the controllable microscope autofocus system structure chart of focal time, comprising: computer (1), camera
(2), article carrying platform (4), X-axis motor (5), y-axis motor (6), Z axis motor (7), light source (8) and microscope (9)；Article carrying platform
(4) sample chips (3) are placed on, the image of camera (2) collecting sample piece (3).
In the present embodiment, the controllable auto focusing device of microscope of focal time uses but is not limited to computer (1)；Figure
It as acquisition device use but is not limited to camera (2), article carrying platform driving device uses but be not limited to driving motor, including X-axis electricity
Machine (5), y-axis motor (6), Z axis motor (7).
A kind of microscope autofocus system that focal time is controllable, comprising:
Computer (1) is connect with camera (2), X-axis motor (5), y-axis motor (6), Z axis motor (7), X-axis motor respectively
(5), y-axis motor (6), Z axis motor (7) are connect with article carrying platform (4)；
Computer (1) is moved to a focusing surface position for controlling article carrying platform；It is adopted by control focal time control
The image for collecting different location, receives the image information of acquisition, and calculate its gradient value；Greatest gradient value corresponding position is exported, and
Save as present convergence face position.
Camera (2) is transmitted to the focal time for acquiring the image information that article carrying platform is moved to designated position
Controllable auto focusing device of microscope；
Z axis motor (7) is used to receive the order-driven loading of the controllable auto focusing device of microscope of the focal time
Platform is mobile.
When being focused operation, steps are as follows:
1, computer (1) control Z axis motor (7) drives article carrying platform (4) to move together, first transports article carrying platform (4)
On the position for moving last focusing；
2, computer (1) control Z axis motor (7) drive article carrying platform (4) move down 40 units (each unit be step
Into a pulse of motor, physical location about 1um)；
3, computer (1) control Z axis motor (7) drives article carrying platform (4) to move up 20 units, computer (1) control
The image of camera (2) collecting sample piece (3) processed, calculates the gradient value of image, and recorded.
4, it repeats step 3 four times；
5, the gradient value of five acquisition images is taken to be compared, the maximum gradient value for clearest image saves the ladder
The corresponding image of angle value as this focus as a result, focus where Z axis position be this focus focusing surface, will be used as down
The reference position once focused.
As shown in figure 4, certain once tests collected five width image and image gradient in whole image gradient curve
Position, it can be seen from the figure that the system improves the accuracy of focusing it is possible to prevente effectively from the case where local extremum focuses,
Improve focusing effect, while the time focused is controllable, improves the whole efficiency of scan sample.
Beneficial effects of the present invention:
1, a kind of focal time of the present invention controllable microscope auto focusing method, system and device effectively solves
The problem of microscope autofocus system focal time of having determined is uncontrollable and focuses easy local extremum, and method is simple easily real
It applies；
2, a kind of focal time of the present invention controllable microscope auto focusing method, system and device, in selection
The initial position that one focusing surface position focuses automatically as microscope effectively reduces automatic focal time, fixed acquisition image
The distance between quantity and fixed acquisition image, effectively control focal time, focusing effect have clear improvement, avoid the occurrence of
The case where local extremum is focused；
3, a kind of focal time of the present invention controllable microscope auto focusing method, system and device, automatic
The image of fixed quantity at equal intervals is acquired in focusing, acquires the interval between image according to micro objective times magnification
Number determines；The product of spacing distance and fixed number magnitude covers all focus points, effectively prevents omitting asking for best focal point
Topic；
4, a kind of focal time of the present invention controllable microscope auto focusing method, system and device, for poly-
Coke is easy to appear local extremum problem, using Z axis stroke scanning constant, solves local focal extreme-value problem, improves focusing effect.
The foregoing is merely preferred embodiment of the present application, are not intended to limit this application, for the skill of this field
For art personnel, various changes and changes are possible in this application.Within the spirit and principles of this application, made any to repair
Change, equivalent replacement, improvement etc., should be included within the scope of protection of this application.Therefore, the present invention is not intended to be limited to this
These embodiments shown in text, and it is to fit to the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. a kind of microscope auto focusing method that focal time is controllable, which is characterized in that this method comprises:
Control article carrying platform is moved to a focusing surface position；
By the image of control focal time control acquisition different location, the image information of acquisition is received, and calculate its gradient value；
Greatest gradient value corresponding position is exported, and saves as present convergence face position.
2. the method as described in claim 1, which is characterized in that described to acquire different location by control focal time control
Image is used in the image for acquiring fixed quantity at equal intervals near the focusing surface.
3. method according to claim 2, which is characterized in that put according to micro objective at the interval between acquisition image
Big multiple determines；The product of spacing distance and fixed number magnitude covers all focus points；For 20 times of object lens of microscope, interval distance
20 μm from selection；Fixed quantity used is 5.
4. method as claimed in claim 3, which is characterized in that receive the focusing surface of acquisition fixed quantity at equal intervals nearby
Image includes:
Control article carrying platform moves down 2 times of spacing distances along Z axis, receives and acquires the location drawing picture；
Control article carrying platform moves up spacing distance along Z axis, receives and acquires the location drawing picture, repeats the step, until acquisition
Complete fixed quantity image.
5. the method as described in claim 1, which is characterized in that in the method, control article carrying platform is moved to a focusing
Before the position of face, it is focused pretreatment；
The focusing pre-processes
The manual acquisition focussing plane of acquisition fixed quantity image at equal intervals nearby is received, gradient curve is converted to；
Judgement focuses the uniqueness of gradient curve extreme point in pretreatment, if uniquely, exporting gradient value corresponding position at extreme point,
And save as focusing surface position；Otherwise, it is focused automatically.
6. method as claimed in claim 5, which is characterized in that in focusing pretreatment, acquire the interval between image
It is determined according to micro objective amplification factor；The product of spacing distance and fixed number magnitude covers all focus points；It is pre- focusing
In processing, for 20 times of object lens of microscope, spacing distance selects 4 μm, and fixed quantity used is 61.
7. method as claimed in claim 5, which is characterized in that in focusing pretreatment, the manual acquisition for receiving acquisition is focused
Nearby fixed quantity image includes: plane at equal intervals
Control article carrying platform moves down 30 times of spacing distances along Z axis, receives and acquires the location drawing picture；
Control article carrying platform moves up spacing distance along Z axis, receives and acquires the location drawing picture, repeats the step, until acquisition
Complete fixed quantity image.
8. a kind of computer readable storage medium, wherein being stored with a plurality of instruction, which is characterized in that described instruction is suitable for by terminal
The processor of equipment equipment loads and executes following processing:
Control article carrying platform is moved to a focusing surface position；
The image of the focusing surface of acquisition nearby fixed quantity at equal intervals is received, and calculates its gradient value；
Greatest gradient value corresponding position is exported, and saves as focusing surface position.
9. a kind of auto focusing device of microscope that focal time is controllable, using computing terminal equipment, including processor and calculating
Machine readable storage medium storing program for executing, processor is for realizing each instruction；Computer readable storage medium is for storing a plurality of instruction, feature
It is, described instruction is suitable for being loaded by processor and executing following processing:
Control article carrying platform is moved to a focusing surface position；
The image of the focusing surface of acquisition nearby fixed quantity at equal intervals is received, and calculates its gradient value；
Greatest gradient value corresponding position is exported, and saves as focusing surface position.
10. a kind of microscope autofocus system that focal time is controllable, which is based on as claimed in claim 9 a kind of poly-
Controllable auto focusing device of microscope of burnt time characterized by comprising
The controllable auto focusing device of microscope of focal time is connect with image collecting device, article carrying platform driving device respectively,
The article carrying platform driving device is connect with article carrying platform；
The controllable auto focusing device of microscope of the focal time is moved to a focusing surface position for controlling article carrying platform；
The image of the focusing surface of acquisition nearby fixed quantity at equal intervals is received, and calculates its gradient value；
Greatest gradient value corresponding position is exported, and saves as focusing surface position；
Described image acquisition device is transmitted to the focusing for acquiring the image information that article carrying platform is moved to designated position
Time controllable auto focusing device of microscope；
The instruction that the article carrying platform driving device is used to receive the controllable auto focusing device of microscope of the focal time is driven
Dynamic article carrying platform is mobile.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115220176A (en) * | 2022-07-25 | 2022-10-21 | 浙江天铂云科光电股份有限公司 | Scanning full-process focusing method based on FPGA |
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