CN109752288A - Surface tension apparatus and measuring method - Google Patents
Surface tension apparatus and measuring method Download PDFInfo
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Abstract
The present invention relates to a kind of surface tension apparatus and measuring method, analyzer includes: sample room (1), the bubble (13,13') for accommodating testing liquid (12) and being surrounded by testing liquid (12)；Image collecting device (2) acquires image of the bubble (13,13') under different compression ratios；Image processing apparatus (3), it is connect with described image acquisition device (2) signal, the size of image of the bubble (13,13') under default compression ratio is measured, and the default corresponding surface tension of compression ratio is calculated according to the size.The present invention observes and measures method compared to existing reading microscope, image processing apparatus realizes the dimensional measurement to bubble image by image procossing, not only to the type less-restrictive of testing liquid, and it is also more accurate in the measurement of the various sizes of bubble, and then improve the measurement accuracy of calculated surface tension.
Description
Technical field
The present invention relates to a kind of physical parameter measurement technology more particularly to a kind of surface tension apparatus and measuring methods.
Background technique
Surface tension is surfactant physical parameter, is pulmonary surfactant important physiological function -- surface-active
Key parameter.The measurement of surface tension, the measurement of especially pressure-dependent extremely low surface tension are for lung surface active object
The research of the physiological function of matter preparation and lung is particularly important.
Currently, surface tension test scheme mainly has surface sedimentation balance method, beating bubble surface tension instrument and controllable closed foam type
Surface tension instrument etc..Surface sedimentation balance method is complicated for operation, is influenced by prepare liquid surface temperature, humidity, and sample size is needed greatly, easily to seep
Leakage, it is difficult to measure low surface tension.Beat bubble surface tension instrument it is easy to operate time saving, need sample size small, measurement temperature compared with
Easy to control, the breathing of the flexible simulation alveolar of vesicle is practical, using universal, but due to bubble and places the capillary of sample
Between there are access, prepare liquid may leak, and bubble contacts the dilution that will cause bubble surface sample to be tested with working fluid
And loss, sample is long in the time that bubble surface is evenly distributed, and influences measuring accuracy.
Controllable closed foam type surface tension instrument by thermostatic water bath, testing liquid around the sample room of bubble, reading microscope, plus
The composition such as pressure device, pressure measuring unit, great advantage are with agar gel for support, and closed vesicle is surrounded by testing liquid,
The leakage of prepare liquid is completely avoided, balance can be quickly reached after vesicle compression, be the best surface tension test of current performance
Method operates easier.But there are still following defects for this method:
1, when testing liquid is suspension (most lung function physiological samples and lung surface active preparation be suspension),
The diameter and height that vesicle can not be accurately measured by reading microscope, influence test accuracy.
2, pressurization poor operability, can not achieve the pressure adjustment of more fine granulation, and cannot in real time, completely record
The pressure-dependent form of vesicle, volume, the situation of change of surface area.
Summary of the invention
The purpose of the present invention is to propose to a kind of surface tension apparatus and measuring methods, are able to ascend stalagmometry
Accuracy.
To achieve the above object, the present invention provides a kind of surface tension apparatus, comprising:
Sample room, the bubble for accommodating testing liquid and being surrounded by testing liquid；
Image collecting device acquires image of the bubble under different compression ratios；
Image processing apparatus is connect with described image acquisition device signal, measures the bubble under default compression ratio
The size of image, and the default corresponding surface tension of compression ratio is calculated according to the size.
Further, further includes: pressurizing device, and the operable connection in the sample room, it is indoor to the sample to
It surveys liquid to pressurize, to adjust the compression ratio of the bubble.
Further, the pressurizing device includes: gas source and pressure regulator valve, and the gas source is via the pressure regulator valve and the sample
The connection of product room, and the gas source is controlled to the amount of pressurization of the sample room by the pressure regulator valve.
Further, the pressurizing device further includes pressure maintaining valve, for stablizing the amount of pressurization for being supplied to the sample room.
Further, described image acquisition device include can automatic focusing camera, automatic focus adjustable can be passed through
To acquire the clear image of the bubble.
Further, described image acquisition device further includes movable supporting frame, and the camera is mounted on described removable
It, being capable of position of the camera relative to the sample room described in adjust automatically on bracket.
Further, the sample room includes closed injection device, accommodates testing liquid in the closed injection device, and
Agar gel supporting surface is equipped between testing liquid and the top of the closed injection device, the bubble is located at the agar gel
The lower section of supporting surface, and be separated with the agar gel supporting surface by testing liquid.
Further, further include pressurizing device with the operable connection in the lower end of the sample room, in the sample room
The lower section of the interior bubble is additionally provided with semi-permeable membrane between the sample room and the pressurizing device.
Further, the sample room has a variety of different loading amount specifications, can be carried out according to the sample size of testing liquid
The selection of corresponding loading amount specification
Further, described image processing unit includes:
Bubble size measuring unit measures the diameter dimension and height gauge of image of the bubble under default compression ratio
It is very little；
Surface tension calculates unit, calculates the corresponding surface of default compression ratio according to the diameter dimension and height dimension
Power.
Further, further includes: pressurizing device, and the operable connection in the sample room, it is indoor to the sample to
It surveys liquid to pressurize, to adjust the compression ratio of the bubble；
Described image processing unit further include:
Compression ratio computing unit, based on integral cutting mathematical model according to the diameter dimension and height dimension calculating
The current compression ratio of bubble；
Regulation Control unit controls the amount of pressurization of the pressurizing device according to the current compression ratio of the bubble, so that institute
The compression ratio for stating bubble is adjusted to default compression ratio.
Further, further include it is following at least one:
Storage unit saves the image of the bubble according to the image acquisition time sequence of the bubble；
Lead-out unit, the measurement data of bubble image, described image processing unit that described image acquisition device is acquired
And calculated result export.
To achieve the above object, the present invention provides a kind of measuring methods based on aforementioned surfaces tension measuring device, comprising:
The sample room for accommodating testing liquid and the bubble surrounded by testing liquid is provided；
Pass through image of the bubble described in image acquisition device under default compression ratio；
The size of image of the bubble under default compression ratio is measured by image processing apparatus, and according to the size
Calculate the default corresponding surface tension of compression ratio.
Further, the surface tension apparatus further includes that can pressurize to the indoor testing liquid of the sample
To adjust the compression ratio pressurizing device of the bubble；
The bubble is being acquired before the operation of the image under default compression ratio, further includes:
Described image processing unit is based on integral cutting mathematical model and calculates institute according to the diameter dimension and height dimension
The current compression ratio of bubble is stated, and controls the amount of pressurization of the pressurizing device according to the current compression ratio of the bubble, so that institute
The compression ratio for stating bubble is adjusted to default compression ratio.
Further, described image acquisition device include can automatic focusing camera；Acquire the image of the bubble
Operation specifically includes:
The camera acquires the clear image of the bubble by automatic focus adjustable.
Based on the above-mentioned technical proposal, the gas that the present invention is surrounded by testing liquid in image acquisition device sample room
The image under different compression ratios is steeped, and measures the size of image of the bubble under default compression ratio by image processing apparatus,
The default corresponding surface tension of compression ratio is calculated further according to the size.Method is observed and measured compared to existing reading microscope,
Image processing apparatus realizes the dimensional measurement to bubble image by image procossing, not only to the limitation of the type of testing liquid compared with
It is few and also more accurate in the measurement of the various sizes of bubble, and then the measurement for improving calculated surface tension is quasi-
True property.
Detailed description of the invention
The drawings described herein are used to provide a further understanding of the present invention, constitutes part of this application, this hair
Bright illustrative embodiments and their description are used to explain the present invention, and are not constituted improper limitations of the present invention.In the accompanying drawings:
Fig. 1 is the functional block diagram of an embodiment of surface tension apparatus of the present invention.
Fig. 2 is the functional block diagram of another embodiment of surface tension apparatus of the present invention.
Fig. 3 is that the sample room of surface tension apparatus embodiment of the present invention is in the schematic diagram of non-pressurized state.
Fig. 4 is that the sample room of surface tension apparatus embodiment of the present invention is in the schematic diagram of pressurized state.
Fig. 5 is the measurement data of the bubble image of testing liquid under normal pressure in surface tension apparatus embodiment of the present invention
Schematic diagram.
Fig. 6 is the measurement data of the bubble image of testing liquid under elevated pressure in surface tension apparatus embodiment of the present invention
Schematic diagram.
Fig. 7 is the flow diagram of an embodiment of the measuring method based on surface tension apparatus embodiment of the present invention.
Fig. 8 is the process signal of another embodiment of the measuring method based on surface tension apparatus embodiment of the present invention
Figure.
Specific embodiment
Below by drawings and examples, technical scheme of the present invention will be described in further detail.
As shown in Figure 1, the functional block diagram of the embodiment for surface tension apparatus of the present invention.In conjunction with Fig. 3-figure
6, the surface tension apparatus of the present embodiment includes: sample room 1, image collecting device 2 and image processing apparatus 3.Wherein, sample
Bubble 13, the 13' that room 1 can accommodate testing liquid 12 and be surrounded by testing liquid 12.For example, sample room 1 may include
Closed injection device 11 accommodates testing liquid 12 in closed injection device 11.In order to avoid the leakage of testing liquid 12, Ke Yi
Agar gel supporting surface (not shown) is set between testing liquid 12 and the top of closed injection device 11.Bubble 13,13'
It is separated in the lower section of agar gel supporting surface, and with agar gel supporting surface by testing liquid.In the branch of agar gel
Support and under the encirclement of testing liquid 12, avoided the leakage of testing liquid and the extension of bubble and bubble periphery sample
Dilution.
Single loading amount specification can be used in sample room 1, in another embodiment, it is possible to have a variety of different loading amounts
Specification, user can select the sample room 1 of corresponding loading amount specification according to the sample size of testing liquid 12, such as 1ml, 2ml,
5ml, 10ml etc..It may include various pulmonary surfactant (Pulmonary in applicable testing liquid 12
Surfactant, abbreviation PS), to be used to realize the measurement of PS agent in vitro surface-active, and realize that some PS lack animal
The stalagmometry of the lung sample of model, or different physiological status people lung function sample measurement.In addition, of the invention
Surface tension apparatus can also be applied to all kinds of testing liquids for needing surface tension.
With reference to another embodiment shown in Fig. 2, sample room 1 be can be set in constant temperature mechanism 4 (such as thermostatic water bath etc.),
To keep the constant temperature of testing liquid in sample room 1.In Fig. 2 constant temperature mechanism also with agitating device 6 (such as water circulating pump etc.) and add
Thermal 7 (such as heating tube etc.) connection, heats the liquid medium in constant temperature mechanism using heating device 7, and utilize
Agitating device 6 makes entire 4 temperature of constant temperature mechanism uniform.By the temperature measure and control to constant temperature mechanism 4, sample can be made
The temperature error of room 1 maintains within a preset range (such as ± 0.2 DEG C etc.).The temperature of sample room 1 can on the control panel into
Row display and setting.
In Fig. 1 embodiment, image collecting device 2 is for acquiring the bubble 13,13' in different compression ratio (i.e. bubbles
Be compressed to the percentage of original volume) under image.Compared to existing in such a way that reading microscope is estimated, this
The collected bubble images of the institute of image collecting device 2 computer of being more convenient for is handled in embodiment.With reference to shown in Fig. 2 another
Embodiment, image collecting device 2 may include can automatic focusing camera 21, gas can be acquired by automatic focus adjustable
The clear image of bubble 13,13'.Here the camera 21 of high magnification numbe may be selected in image collecting device 2, so as to obtain more
Bubble image detail.Also, in order to be more clear bubble image, the automatic focusing function that can use camera 21 is carried out
Focussing is subsequent dimensional measurement and the accuracy that parameter calculates to obtain the clear image of testing liquid and bubble
Basis is provided.In Fig. 2, constant temperature mechanism 4 can also connect lighting device 5, be that camera 21 provides environment using lighting device 5
Light, so that the shooting image of testing liquid and bubble is more clear.
Camera 21 can be fixed relative to the position of sample room 1, also be can according to need and be adjusted.With reference to Fig. 2,
It can also include movable supporting frame 22, such as removable three coordinated holders adjusted in image collecting device 2.Camera 21 can be with
It is mounted on movable supporting frame 22, image collecting device 2 can need adjust automatically camera 21 relative to sample according to shooting
The position of room 1.
In Fig. 1 embodiment, image processing apparatus 3 is connect with 2 signal of described image acquisition device, for measuring the gas
The size of the image of bubble 13,13' under default compression ratio, and the corresponding surface of default compression ratio is calculated according to the size
Power.Image processing apparatus 3 can accurately measure 12 ring of testing liquid by the scale of computer after obtaining bubble image
Around the size of bubble 13,13', such as bubble diameter D and bubble height H.Bubble can be calculated default according to these sizes
The corresponding surface tension of compression ratio, default compression ratio here can be selected according to testing liquid.
For PS, major physiological effect is exactly the surface tension for reducing alveolar gas-liquid interface, surface tension with
The variation of alveolar surface area (or volume) size and change.And lowest surface tension is the peculiar concept of pulmonary surfactant, is
Characterize the proprietary index of pulmonary surfactant and lung physiological function relationship, that is to say, that lowest surface tension and its physiological activity
It is directly related.Therefore alveolar contraction need to be simulated, attainable lowest surface tension when bubble compression, surface area diminution is understood.Example
Such as when alveolar is compressed to the 40% of total lung capacity, alveolar surface tension is bordering on zero, therefore when the surface tension of the present embodiment is surveyed
Instrument is determined when carrying out the measurement of surface tension, the bubble compression ratio that testing liquid 12 surrounds can be controlled 40%, be led at this time
Crossing and measuring the surface tension that the size of bubble is calculated is default compression ratio (40%) corresponding minimum surface
Power.For other testing liquids, default compression ratio is also possible to other numerical value, such as 20%, 60% etc..
Existing surface tension test scheme is usually to carry out in non-pressurized situation, at this time the volume and surface area of bubble
Do not change.But in order to understand the surface characteristic of PS, the feature of simulation alveolar surface PS will be in bubble surface area or stereomutation
In the case where observe the change of surface tension, therefore as Control of drug quality and lung function sample measures, it is to be understood that bubble
The lowest surface tension that can reach when compressing to a certain extent.In this way, the compression in order to realize bubble in sample room 1, ginseng
Another embodiment shown in Fig. 2 is examined, surface tension apparatus can also include pressurizing device 8, can operate with the sample room 1
Connection, pressurize to the testing liquid 12 in the sample room 1, to adjust the compression ratio of the bubble 13,13'.Pressurization
Device 8 can be pressurizeed by compressed gas, and adjust pressure size as needed, so as to the life of accurate characterization sample to be tested
Manage function.
In Fig. 2, pressurizing device 8 may include gas source 81 and pressure regulator valve 82.Wherein, gas source 81 is via pressure regulator valve 82 and sample
Product room 1 is connected to, and controls gas source 81 to the amount of pressurization of sample room 1 by pressure regulator valve 82.Gas source 81 can be by the pressure in compressed gas cylinder
Contracting air or nitrogen provide, and pressure regulator valve 82 can be realized the control of more accurate pressurization and decompression, and operability is stronger, and
And it can be realized the pressure adjustment of more fine granulation, and then can be realized vesicle pressure-dependent form, volume, surface area
The real-time record of situation of change obtains and more completely records result.Pressurizing device 8 can also include pressure maintaining valve 83, for stablizing
It is supplied to the amount of pressurization of the sample room 1, when to make the different samples for measuring or measuring every time, different batches same sample
It is all measured under identical pressure condition, data have more comparativity.It can also include that pressure detecting is shown in pressurizing device 8
Device 84 is able to detect the pressure of pressurizing device 8 and is shown.
In another embodiment, the lower end of sample room 1 can be with the connection of 8 operability of pressurizing device.Surface tension
Analyzer can also include semi-permeable membrane, by semi-permeable membrane carry out testing liquid 12 barrier, can conducting pressure and prevent to
The active component for surveying liquid 12 leaks into outside syringe.Moreover, semi-permeable membrane can be closer to the position of bubble, such as in sample room
It bubble 13, the lower section of 13' or is configured between sample room 1 and pressurizing device 8 in 1, to avoid sample from being diluted
While, it can be realized the measurement of the smaller physiologically sample of specimen amount.
Image processing apparatus 3 can be realized by computer, and can pass through multiple hardware or software realization.Such as image
Processing unit 3 can specifically include: bubble size measuring unit, measure the image of the bubble 13,13' under default compression ratio
Diameter dimension D and height dimension H；Unit is calculated with surface tension, is calculated according to the diameter dimension D and height dimension H pre-
If the corresponding surface tension of compression ratio.
Calculating for surface tension, the article " Interfacial delivered according to J.D.Malcolm and C.D.Elliott
tension from height and diameter of a single profile drop or captive bubble”
It is found that symmetrical bubble formed in solution, when its radius of curvature is more sufficiently large than film thickness, transmural pressure Δ P and surface tension r
It can be indicated by Laplace equation are as follows:
r×(1/R1- 1/R2)=Δ P, wherein R1, R2For radius of curvature.
J.D.Malcolm and C.D.Elliot handles closed air bubbles with above equation by Runge Kutta method, obtains
Following result:
R=Δ ρ × g × [H/G (H/D)]2
Wherein, Δ ρ is the density contrast of substance inside and outside bubble, and g is acceleration of gravity,
G (H/D)=C1+C2×(H/D)1+C3×(H/D)2+C4×(H/D)3+C5×(H/D)4
Wherein, C1~C5For coefficient, H is bubble height, and D is bubble diameter.
C1~C5Related with D/H range, as shown in the table, D/H is in different range, between G (H/D) and measurement data
Deviation is also different, is able to satisfy the requirement of Curosurf similar drug and lung physiological function sample surface tension test.It calculates
When can need to select suitable condition and range according to specific experiments.
| C1 | C2 | C3 | C4 | C5 | D/H | Inequality, % |
| 1.86519 | 2.807066 | -9.430927 | 8.669726 | -3.6606 | 1.14-10.23 | 0.041 |
| 1.79559 | 3.44148 | -11.38556 | 11.13387 | -4.7502 | 1.14-6.60 | 0.028 |
| 2.11500 | 0.46986 | -2.315248 | 0 | 0 | 1.14-6.60 | 0.142 |
| 1.37436 | 6.52959 | -19.47756 | 20.15582 | -8.3783 | 1.14-3.10 | 0.024 |
| 2.35019 | -0.5878 | -0.852229 | -0.63065 | 0 | 1.14-3.10 | 0.080 |
| 2.22448 | 0.09538 | -2.01974 | 0 | 0 | 1.14-3.10 | 0.091 |
On this basis, in order to be accurately controlled the pressure effect of sample room, in another embodiment, at described image
Managing device 3 further includes compression ratio computing unit and pressure regulation unit.Wherein, compression ratio computing unit can be based on integral cutting mathematics
Model calculates the current compression ratio of bubble 13,13' according to diameter dimension and height dimension.It is this that mathematical model is cut based on integral
Calculation be that the position to be connected based on bubble in clear image with testing liquid carries out integral cutting, and further calculates
Volume, surface area and compression ratio out, it is more accurate compared with existing more coarse computation model.Pressure regulation unit is used for basis
The bubble 13,13' current compression ratio adjust the amount of pressurization of the pressurizing device 8 so that the compression of the bubble 13,13'
Than being adjusted to default compression ratio.The compression that pressure regulation unit can be such that different amount of pressurization measure by the process for pressure release of repeatedly pressurizeing
Than gradually approaching default compression ratio.
It in another embodiment, can also further comprise storage unit, lead-out unit in surface tension apparatus
At least one of.Wherein, storage unit can according to the bubble 13,13' image acquisition time sequence to the bubble
13, the image of 13' is saved.Corresponded to image acquisition time bubble image can after being saved as initial data for
Retrospect, to meet the requirement of initial data authenticity and integrity.Lead-out unit, which can according to need, adopts image collecting device 2
Bubble 13,13' image, the measurement data of described image processing unit 3 and the calculated result export of collection.
Based on each embodiment of aforementioned surface tension apparatus of the present invention, the present invention also provides the surveys of corresponding surface tension
Method is determined, as shown in fig. 7, the process for an embodiment of the measuring method based on surface tension apparatus embodiment of the present invention is shown
It is intended to.In the present embodiment, measuring method specifically includes:
Step 101 provides the sample room 1 for accommodating testing liquid 12 and the bubble 13,13' that are surrounded by testing liquid 12；
Step 102 acquires the image of the bubble 13,13' under default compression ratio by image collecting device 2；
Step 103, the ruler that the image of the bubble 13,13' under default compression ratio is measured by image processing apparatus 3
It is very little, and the default corresponding surface tension of compression ratio is calculated according to the size.
In order to make the bubble in sample room 1 reach default compression ratio, preferably further comprise in surface tension apparatus
It can be pressurizeed to the testing liquid 12 in the sample room 1 to adjust the bubble 13, the pressurizing device of the compression ratio of 13'
8.Correspondingly, can also include: that image processing apparatus 3 is based on integral cutting mathematical model according to described straight before step 102
Diameter size and the height dimension calculating bubble 13, the current compression ratio of 13', and according to the bubble 13, the current compression of 13'
Than the amount of pressurization for controlling the pressurizing device 8, so that the bubble 13, the compression ratio of 13' is adjusted to default compression ratio.
As shown in figure 8, the stream of another embodiment for the measuring method based on surface tension apparatus embodiment of the present invention
Journey schematic diagram.Compared with a upper embodiment, image collecting device 2 include can automatic focusing camera 21.Correspondingly, step
102 can specifically include: step 102 ', camera 21 bubble 13, the clear figure of 13' are acquired by automatic focus adjustable
Picture.
It is said below with reference to continuous mode example of the surface tension apparatus embodiment shown in Fig. 2 to surface tension
It is bright.
The sample of testing liquid is diluted to suitable concentration, and opens surface tension apparatus, heating device, stirring dress
It sets, while starting the computer for realizing image processing apparatus, camera is attached with computer by suitable interface.
The available loading amount specification of surface tension apparatus is selected according to the sample size of sample to be tested, and prepares suitable concentration
Agar solution, appropriate injection sample room is taken out after heating and dissolution.Syringe port as sample room is upward and holding is flat
It is whole, to agar slowly cooled and solidified, after agar solidification, sample to be tested is taken to inject sample room, and reserve one and be of moderate size
Bubble (non-pressurized condition as shown in Figure 3).
Testing liquid will be filled it up in tubule equipped with threeway, and connected with sample room, is inverted into constant temperature mechanism 4, so
After fasten each valve, open threeway, gas source, and pressure is adjusted by pressure regulator valve, prepare to measure.Until constant temperature mechanism is heated to
When preset temperature, lighting device is opened, the focal length of the adjustable camera of movable supporting frame of camera cooperation at this time, so that bubble
It is high-visible.Using the corresponding pixel of preset length (such as 1mm etc.) as scale, demarcates and saves, then take pictures and acquire image,
And automatic measurement bubble height and bubble diameter (2.836 millimeters of the height of bubble 13 shown in Figure 5 and straight in a computer
5.135 millimeters of diameter).
Start uniform pressurization, then empty, repressurization, empty, repeats this process so that bubble reaches default compression
Than (such as 40%).In each pressurization, is all taken pictures, measured, and calculate the compression ratio of bubble.Pressure regulator valve can be according to every
Gap between bubble compression ratio and default compression ratio when secondary pressurization carries out the adjustment of amount of pressurization, by primary or multiple
Adjust to obtain the amount of pressurization for corresponding to default compression ratio (1.358 millimeters of the height of bubble 13 ' shown in Figure 6 and
4.866 millimeters of diameter).Under default compression ratio, the size for bubble of taking pictures, measure, and substitute into aforementioned formula and calculate surface
Power.
Finally it should be noted that: the above embodiments are merely illustrative of the technical scheme of the present invention and are not intended to be limiting thereof；To the greatest extent
The present invention is described in detail with reference to preferred embodiments for pipe, it should be understood by those ordinary skilled in the art that: still
It can modify to a specific embodiment of the invention or some technical features can be equivalently replaced；Without departing from this hair
The spirit of bright technical solution should all cover within the scope of the technical scheme claimed by the invention.
Claims (15)
1. a kind of surface tension apparatus characterized by comprising
Sample room (1), the bubble (13,13') for accommodating testing liquid (12) and being surrounded by testing liquid (12)；
Image collecting device (2) acquires image of the bubble (13,13') under different compression ratios；
Image processing apparatus (3) is connect with described image acquisition device (2) signal, measures the bubble (13,13') default
The size of image under compression ratio, and the default corresponding surface tension of compression ratio is calculated according to the size.
2. surface tension apparatus according to claim 1, which is characterized in that further include: pressurizing device (8), and it is described
Sample room (1) operable connection pressurizes to the testing liquid (12) in the sample room (1), to adjust the bubble
The compression ratio of (13,13').
3. surface tension apparatus according to claim 2, which is characterized in that the pressurizing device (8) includes: gas source
(81) it is connected to via the pressure regulator valve (82) with the sample room (1) with pressure regulator valve (82), the gas source (81), and by described
Pressure regulator valve (82) controls the gas source (81) to the amount of pressurization of the sample room (1).
4. surface tension apparatus according to claim 3, which is characterized in that the pressurizing device (8) further includes pressure stabilizing
Valve (83), for stablizing the amount of pressurization for being supplied to the sample room (1).
5. surface tension apparatus according to claim 1, which is characterized in that described image acquisition device (2) includes can
The camera (21) of automatic focusing, can acquire the clear image of the bubble (13,13') by automatic focus adjustable.
6. surface tension apparatus according to claim 5, which is characterized in that described image acquisition device (2) further includes
Movable supporting frame (22), the camera (21) are mounted on the movable supporting frame (22), can image described in adjust automatically
Position of the head (21) relative to the sample room (1).
7. surface tension apparatus according to claim 1, which is characterized in that the sample room (1) includes closed injection
Device (11) accommodates testing liquid (12) in the closed injection device (11), and in testing liquid (12) and the closed injection
Agar gel supporting surface is equipped between the top of device (11), the bubble (13,13') is located under the agar gel supporting surface
Side, and be separated with the agar gel supporting surface by testing liquid.
8. surface tension apparatus according to claim 7, which is characterized in that further include under the sample room (1)
The pressurizing device (8) for holding operable connection, the lower section of the bubble (13,13') or described in the sample room (1)
Semi-permeable membrane is additionally provided between sample room (1) and the pressurizing device (8).
9. surface tension apparatus according to claim 1, which is characterized in that the sample room (1) has a variety of differences
Loading amount specification can carry out the selection of corresponding loading amount specification according to the sample size of testing liquid (12).
10. surface tension apparatus according to claim 1, which is characterized in that described image processing unit (3) includes:
Bubble size measuring unit measures the diameter dimension and height of image of the bubble (13,13') under default compression ratio
Size；
Surface tension calculates unit, calculates the corresponding minimum surface of default compression ratio according to the diameter dimension and height dimension
Power.
11. surface tension apparatus according to claim 10, which is characterized in that further include: pressurizing device (8), with institute
Sample room (1) operable connection is stated, is pressurizeed to the testing liquid (12) in the sample room (1), to adjust the gas
Steep the compression ratio of (13,13')；
Described image processing unit (3) further include:
Compression ratio computing unit calculates the bubble according to the diameter dimension and height dimension based on integral cutting mathematical model
The current compression ratio of (13,13')；
Pressure regulation unit adjusts the amount of pressurization of the pressurizing device (8) according to the current compression ratio of the bubble (13,13'), so that
The compression ratio of the bubble (13,13') is adjusted to default compression ratio.
12. surface tension apparatus according to claim 1, which is characterized in that further include it is following at least one:
Storage unit, according to the bubble (13,13') image acquisition time sequence to the image of the bubble (13,13') into
Row saves；
Lead-out unit, by bubble (13,13') image of described image acquisition device (2) acquisition, described image processing unit (3)
Measurement data and calculated result export.
13. a kind of lowest surface tension preparation method based on any surface tension apparatus of claim 1~12,
It is characterized in that, comprising:
The sample room (1) for accommodating testing liquid (12) and the bubble (13,13') surrounded by testing liquid (12) is provided；
Image of the bubble (13,13') under default compression ratio is acquired by image collecting device (2)；
By the size of image of image processing apparatus (3) measurement bubble (13,13') under default compression ratio, and according to
The size calculates the default corresponding surface tension of compression ratio.
14. lowest surface tension preparation method according to claim 13, which is characterized in that the surface tension apparatus
It further include that can be pressurizeed to the testing liquid (12) in the sample room (1) to adjust the compression of the bubble (13,13')
The pressurizing device (8) of ratio；
The bubble (13,13') is being acquired before the operation of the image under default compression ratio, further includes:
Described image processing unit (3) is based on integral cutting mathematical model according to the diameter dimension and height dimension calculating
The current compression ratio of bubble (13,13'), and the pressurizing device is controlled according to the current compression ratio of the bubble (13,13')
(8) amount of pressurization, so that the compression ratio of the bubble (13,13') is adjusted to default compression ratio.
15. lowest surface tension preparation method according to claim 13, which is characterized in that described image acquisition device
(2) include can automatic focusing camera (21)；The operation for acquiring the image of the bubble (13,13') specifically includes:
The camera (21) acquires the clear image of the bubble (13,13') by automatic focus adjustable.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113049454A (en) * | 2021-03-26 | 2021-06-29 | 福州大学 | Method for measuring and calculating dynamic interfacial tension of multi-phase emulsion in micro-channel |
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