CN109749936A - A kind of screening technique of 3- hydracrylic acid producing bacterial strain - Google Patents
A kind of screening technique of 3- hydracrylic acid producing bacterial strain Download PDFInfo
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- CN109749936A CN109749936A CN201910040392.7A CN201910040392A CN109749936A CN 109749936 A CN109749936 A CN 109749936A CN 201910040392 A CN201910040392 A CN 201910040392A CN 109749936 A CN109749936 A CN 109749936A
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- hydracrylic acid
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Abstract
The present invention relates to chemical technology fields, especially a kind of screening technique of 3- hydracrylic acid producing bacterial strain, including 1), Enriching soil strain: take 5 parts of 1g soil samples in 5 equipped with 100mL enriched medium 250mL triangular flask in, 37 DEG C of shaking table shaken cultivations, then 180 r/min cultivate 24 h；5mL enrichment culture liquid is taken to be transferred in every utilization of carbon source culture medium respectively again, 250mL triangular flask liquid amount 100mL, 37 DEG C of shaking table 180r/min shaken cultivation 1-2d；2), the processing of culture solution: the culture solution 5mL for cultivating completion in step 1) is taken, then supernatant is taken with 2000r/min centrifugation 5min, is handled with 732 cation exchange resin of strong-acid type, the sample for handling completion is to be measured；3) it, detects aimed strain: first reusing in paper chromatography test sample whether have aimed strain using HPLC analytic approach.The present invention obtains the screening technique of 3- hydracrylic acid producing bacterial strain, and reaction system is simple by the test of a variety of screening carbon sources, and cost is relatively low, can help to the development of correlative study work, has highly important application value.
Description
Technical field
The present invention relates to chemical technology field more particularly to a kind of screening techniques of 3- hydracrylic acid producing bacterial strain.
Background technique
3- hydracrylic acid (3-hydroxypropionic acid, write a Chinese character in simplified form 3-HP) is that there are three the non-of carbon atom for a kind of tool
Chiral organic acid, acid ionization constant (pKa) are 4.5, are in a liquid state, have stickiness, colorless and odorless, water-soluble, ethyl alcohol, ether.
3- hydracrylic acid is respectively provided with 1 carboxyl and 1 hydroxyl at molecule both ends, is more more active than its isomer lactic acid
Molecule, is industrially used to many important chemical products of synthesis, and such as hydrogenation becomes 1,3 propylene glycol, is oxidized to malonic acid, takes off
Water obtains acrylic acid, and be polymerized to high molecular material etc., it is a kind of important chemical industry platform product.
Currently, 3-HP in the market is produced using non-renewable fossil resources as raw material using chemical synthesis, difficulty
It is larger, and separation and purification of products is more complex, high production cost.Both at home and abroad carried out micro target metabolic product in nature and
The exploration of the method for detecting of relevant bacteria species, therefore a kind of screening technique of 3- hydracrylic acid producing bacterial strain of this experiment proposition.
Summary of the invention
The purpose of the present invention is to solve separation and purification of products in the prior art is more complex, the shortcomings that high production cost,
And a kind of screening technique of the 3- hydracrylic acid producing bacterial strain proposed.
To achieve the goals above, present invention employs following technical solutions:
Design a kind of screening technique of 3- hydracrylic acid producing bacterial strain, comprising the following steps:
1), Enriching soil strain: taking 5 parts of 1g soil samples in the triangular flask of 5 250mL equipped with 100mL enriched medium, and 37
DEG C shaking table shaken cultivation, then 180 r/min cultivate 24 h；5mL enrichment culture liquid is taken to be transferred to every utilization of carbon source training respectively again
It supports in base, 250mL triangular flask liquid amount 100mL, 37 DEG C of shaking table 180r/min shaken cultivation 1-2d；
2), the processing of culture solution: the culture solution 5mL for cultivating completion in step 1) is taken, then is taken with 2000r/min centrifugation 5min
Clear liquid is handled with 732 cation exchange resin of strong-acid type, and the sample for handling completion is to be measured；
3) it, detects aimed strain: first reusing in paper chromatography test sample whether have aimed strain using HPLC analytic approach；
4), the purifying of bacterial strain: the bacterium colony that the sample detected in step 3) obtains is placed on the inorganic salts containing terramycin and is consolidated
It crosses on body culture medium flat plate, is separated and purified；This step repeats 2-3 times, until obtaining single bacterium colony；Then by single bacterium
It falls in inorganic salt liquid culture medium of the access containing terramycin, is placed in 30-38 DEG C, under the conditions of 150r/min concussion, shading culture
36-48h obtains single colonie culture solution；
5), the extraction of aimed strain: the culture solution crude product solution containing aimed strain and organic solvent are sufficiently mixed, steaming is passed through
The method evaporated obtains the 3- hydracrylic acid of the organic phase containing 3- hydracrylic acid or purifying.
Preferably, the soil sample in the step 1) first removes 5cm surface soil in sampling, acquires the soil of 5-15cm depth.
Preferably, the enriched medium be by tryptone 1.7, soya peptone 0.3, glucose 2.5, Na0H5,
K2HP04 2.5, water 100,7.5,115 DEG C of steam ash bacterium 20min of pH 7.1- are made.
Preferably, the utilization of carbon source culture medium be by casein peptone 0.5, glucose 0.25, NaC10.5,
K2HP040.25, carbon source 1.5,100, pH7.5,115 DEG C of steam sterilizing 20min of water are made.
Preferably, the carbon source in the step 1) includes glucose, 1,2-PD, 1,3-PD, glycerol, xylose.
Preferably, the basic catalyst that the concussion in the step 4) is added is hydroxy homogeneous by that can ionize out in water
With multiphase alkalinity material composition.
Preferably, the basic catalyst be sodium hydroxide, calcium hydroxide, organic amine, magnesium hydroxide, magnalium hydrotalcite,
One of alkaline molecular sieve, aluminium oxide are a variety of.
Preferably, the organic solvent in the step 1) is by esters, ketone, alcohols, phosphoric acid ester, phosphorus oxygen class, phosphine sulphur
One of class, amine and other organic compounds or a variety of compositions, and the boiling point of the organic compound is big under normal pressure
In 100 DEG C.
A kind of screening technique of 3- hydracrylic acid producing bacterial strain proposed by the present invention, beneficial effect are: the present invention passes through
The test of a variety of screening carbon sources, obtains the screening technique of 3- hydracrylic acid producing bacterial strain, and reaction system is simple, and cost is relatively low,
The development that can help to correlative study work, is easy to industrialize, and has highly important application value.
Specific embodiment
The following is a clear and complete description of the technical scheme in the embodiments of the invention, it is clear that described embodiment
Only a part of the embodiment of the present invention, instead of all the embodiments.
Embodiment 1
A kind of screening technique of 3- hydracrylic acid producing bacterial strain, comprising the following steps:
1), Enriching soil strain: taking 5 parts of 1g soil samples in the triangular flask of 5 250mL equipped with 100mL enriched medium, and 37
DEG C shaking table shaken cultivation, then 180 r/min cultivate 24 h；5mL enrichment culture liquid is taken to be transferred to every utilization of carbon source training respectively again
It supports in base, 250mL triangular flask liquid amount 100mL, 37 DEG C of shaking table 180r/min shaken cultivation 1-2d；
2), the processing of culture solution: the culture solution 5mL for cultivating completion in step 1) is taken, then is taken with 2000r/min centrifugation 5min
Clear liquid is handled with 732 cation exchange resin of strong-acid type, and the sample for handling completion is to be measured；
3) it, detects aimed strain: first reusing in paper chromatography test sample whether have aimed strain using HPLC analytic approach；
4), the purifying of bacterial strain: the bacterium colony that the sample detected in step 3) obtains is placed on the inorganic salts containing terramycin and is consolidated
It crosses on body culture medium flat plate, is separated and purified；This step repeats 2-3 times, until obtaining single bacterium colony；Then by single bacterium
It falls in inorganic salt liquid culture medium of the access containing terramycin, is placed in 30 DEG C, under the conditions of 150r/min concussion, shading culture 36h,
Obtain single colonie culture solution；
5), the extraction of aimed strain: the culture solution crude product solution containing aimed strain and organic solvent are sufficiently mixed, steaming is passed through
The method evaporated obtains the 3- hydracrylic acid of the organic phase containing 3- hydracrylic acid or purifying.
Wherein, the soil sample in step 1) first removes 5cm surface soil in sampling, acquires the soil of 5-15cm depth.
Wherein, enriched medium be by tryptone 1.7, soya peptone 0.3, glucose 2.5, Na0H5, K2HP04 2.5,
Water 100,7.5,115 DEG C of steam ash bacterium 20min of pH 7.1- are made.
Wherein, utilization of carbon source culture medium is by casein peptone 0.5, glucose 0.25, NaC10.5, K2HP040.25, carbon source
1.5,100, pH7.5,115 DEG C of steam sterilizing 20min of water are made.
Wherein, the carbon source in step 1) includes glucose, 1,2-PD, 1,3-PD, glycerol, xylose.
Wherein, the basic catalyst that the concussion in step 4) is added by that can ionize out homogeneous and multiphase hydroxy in water
Alkaline matter composition.
Wherein, basic catalyst is sodium hydroxide, calcium hydroxide, organic amine, magnesium hydroxide, magnalium hydrotalcite, alkalinity point
One of son sieve, aluminium oxide are a variety of.
Wherein, the organic solvent in step 1) is by esters, ketone, alcohols, phosphoric acid ester, phosphorus oxygen class, phosphine sulphur class, amine
One of class and other organic compounds or a variety of compositions, and the boiling point of the organic compound is all larger than 100 under normal pressure
℃。
Embodiment 2
A kind of screening technique of 3- hydracrylic acid producing bacterial strain, comprising the following steps:
1), Enriching soil strain: taking 5 parts of 1g soil samples in the triangular flask of 5 250mL equipped with 100mL enriched medium, and 37
DEG C shaking table shaken cultivation, then 180 r/min cultivate 24 h；5mL enrichment culture liquid is taken to be transferred to every utilization of carbon source training respectively again
It supports in base, 250mL triangular flask liquid amount 100mL, 37 DEG C of shaking table 180r/min shaken cultivation 1-2d；
2), the processing of culture solution: the culture solution 5mL for cultivating completion in step 1) is taken, then is taken with 2000r/min centrifugation 5min
Clear liquid is handled with 732 cation exchange resin of strong-acid type, and the sample for handling completion is to be measured；
3) it, detects aimed strain: first reusing in paper chromatography test sample whether have aimed strain using HPLC analytic approach；
4), the purifying of bacterial strain: the bacterium colony that the sample detected in step 3) obtains is placed on the inorganic salts containing terramycin and is consolidated
It crosses on body culture medium flat plate, is separated and purified；This step repeats 2-3 times, until obtaining single bacterium colony；Then by single bacterium
It falls in inorganic salt liquid culture medium of the access containing terramycin, is placed in 35 DEG C, under the conditions of 150r/min concussion, shading culture 42h,
Obtain single colonie culture solution；
5), the extraction of aimed strain: the culture solution crude product solution containing aimed strain and organic solvent are sufficiently mixed, steaming is passed through
The method evaporated obtains the 3- hydracrylic acid of the organic phase containing 3- hydracrylic acid or purifying.
Embodiment 3
A kind of screening technique of 3- hydracrylic acid producing bacterial strain, comprising the following steps:
1), Enriching soil strain: taking 5 parts of 1g soil samples in the triangular flask of 5 250mL equipped with 100mL enriched medium, and 37
DEG C shaking table shaken cultivation, then 180 r/min cultivate 24 h；5mL enrichment culture liquid is taken to be transferred to every utilization of carbon source training respectively again
It supports in base, 250mL triangular flask liquid amount 100mL, 37 DEG C of shaking table 180r/min shaken cultivation 1-2d；
2), the processing of culture solution: the culture solution 5mL for cultivating completion in step 1) is taken, then is taken with 2000r/min centrifugation 5min
Clear liquid is handled with 732 cation exchange resin of strong-acid type, and the sample for handling completion is to be measured；
3) it, detects aimed strain: first reusing in paper chromatography test sample whether have aimed strain using HPLC analytic approach；
4), the purifying of bacterial strain: the bacterium colony that the sample detected in step 3) obtains is placed on the inorganic salts containing terramycin and is consolidated
It crosses on body culture medium flat plate, is separated and purified；This step repeats 2-3 times, until obtaining single bacterium colony；Then by single bacterium
It falls in inorganic salt liquid culture medium of the access containing terramycin, is placed in 38 DEG C, under the conditions of 150r/min concussion, shading culture 48h,
Obtain single colonie culture solution；
5), the extraction of aimed strain: the culture solution crude product solution containing aimed strain and organic solvent are sufficiently mixed, steaming is passed through
The method evaporated obtains the 3- hydracrylic acid of the organic phase containing 3- hydracrylic acid or purifying.
To there is aimed strain, therefore 1,3- in 1,3-PD and glycerol sample in the step 3) of three of the above embodiment
Propylene glycol and glycerol can be used as the screening carbon source of 3- hydracrylic acid producing bacterial strain, while show that 150r/min shakes at 35 DEG C
Under the conditions of, bacterium colony purity highest that shading culture 42h can be purified.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (8)
1. a kind of screening technique of 3- hydracrylic acid producing bacterial strain, it is characterised in that: the following steps are included:
1), Enriching soil strain: taking 5 parts of 1g soil samples in the triangular flask of 5 250mL equipped with 100mL enriched medium, and 37
DEG C shaking table shaken cultivation, then 180 r/min cultivate 24 h；5mL enrichment culture liquid is taken to be transferred to every utilization of carbon source training respectively again
It supports in base, 250mL triangular flask liquid amount 100mL, 37 DEG C of shaking table 180r/min shaken cultivation 1-2d；
2), the processing of culture solution: the culture solution 5mL for cultivating completion in step 1) is taken, then is taken with 2000r/min centrifugation 5min
Clear liquid is handled with 732 cation exchange resin of strong-acid type, and the sample for handling completion is to be measured；
3) it, detects aimed strain: first reusing in paper chromatography test sample whether have aimed strain using HPLC analytic approach；
4), the purifying of bacterial strain: the bacterium colony that the sample detected in step 3) obtains is placed on the inorganic salts containing terramycin and is consolidated
It crosses on body culture medium flat plate, is separated and purified；This step repeats 2-3 times, until obtaining single bacterium colony；Then by single bacterium
It falls in inorganic salt liquid culture medium of the access containing terramycin, is placed in 30-38 DEG C, under the conditions of 150r/min concussion, shading culture
36-48h obtains single colonie culture solution；
5), the extraction of aimed strain: the culture solution crude product solution containing aimed strain and organic solvent are sufficiently mixed, steaming is passed through
The method evaporated obtains the 3- hydracrylic acid of the organic phase containing 3- hydracrylic acid or purifying.
2. a kind of screening technique of 3- hydracrylic acid producing bacterial strain according to claim 1, which is characterized in that the step
1) soil sample in first removes 5cm surface soil in sampling, acquires the soil of 5-15cm depth.
3. a kind of screening technique of 3- hydracrylic acid producing bacterial strain according to claim 1, which is characterized in that the enrichment
Culture medium is by tryptone 1.7, soya peptone 0.3, glucose 2.5, Na0H5, K2HP042.5, water 100, pH 7.1- 7.5,
115 DEG C of steam ash bacterium 20min are made.
4. a kind of screening technique of 3- hydracrylic acid producing bacterial strain according to claim 1, which is characterized in that the carbon source
It is by casein peptone 0.5, glucose 0.25, NaC10.5, K using culture medium2HP040.25, carbon source 1.5, water 100, pH7.5,
115 DEG C of steam sterilizing 20min are made.
5. a kind of screening technique of 3- hydracrylic acid producing bacterial strain according to claim 1, which is characterized in that the step
1) carbon source in includes glucose, 1,2- propylene glycol, 1,3- propylene glycol, glycerol, xylose.
6. a kind of screening technique of 3- hydracrylic acid producing bacterial strain according to claim 1, which is characterized in that the step
4) basic catalyst that the concussion in is added by that can ionize out homogeneous and multiphase alkalinity material composition hydroxy in water.
7. a kind of screening technique of 3- hydracrylic acid producing bacterial strain according to claim 6, which is characterized in that the alkalinity
Catalyst is sodium hydroxide, calcium hydroxide, organic amine, magnesium hydroxide, magnalium hydrotalcite, alkaline molecular sieve, one in aluminium oxide
Kind is a variety of.
8. a kind of screening technique of 3- hydracrylic acid producing bacterial strain according to claim 1, which is characterized in that the step
1) organic solvent in is by esters, ketone, alcohols, phosphoric acid ester, phosphorus oxygen class, phosphine sulphur class, amine and other organic compounds
One of or a variety of compositions, and the boiling point of the organic compound is all larger than 100 DEG C under normal pressure.
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