CN109738404A - A kind of method and device of quick screening fluorescence spectrum peak value batch data - Google Patents
A kind of method and device of quick screening fluorescence spectrum peak value batch data Download PDFInfo
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Abstract
The application provides a kind of method and device of quickly screening fluorescence spectrum peak value batch data.The described method includes: obtaining the three-dimensional fluorescence spectrum data of at least one sample to be analyzed;Obtain the fluorescence emission wavelengths range and excitation wavelength range of at least one DOM component;Fluorescence spectrum peak in the current display picture of display component for every group of spectroscopic data carries out location point calibration, and carries out borderline region delimitation for each DOM component;The location point that fluorescence spectrum peak whether is distributed in each borderline region is detected, and determines the target fluorescent spectrum peak of each DOM component according to testing result;Data summarization is carried out to all target fluorescent spectrum peaks and obtains fluorescence spectrum peak the selection result.The method operation difficulty is low, and generalization is strong, can quickly and accurately determine the fluorescence spectrum peak distributing position of items DOM component in all samples to be analyzed, and provides corresponding effect of visualization, intuitively to realize entire fluorescence spectrum peak screening process.
Description
Technical field
This application involves three-dimensional fluorescence spectrum data screening technical fields, in particular to a kind of quickly screening fluorescence
The method and device of spectrum peak batch data.
Background technique
With the continuous development of science and technology, three-dimensional fluorescence spectrum (Three-Dimensional Excitation-
Emission Matrix Fluorescence Spectra, 3DEEM) technology is because its amount of samples is few, repeated high, sensitivity
It is high and do not destroy the advantages such as sample structure, it is widely used in organic matter (Organic Matter, OM) test field, is used for
It treats test material sample (for example, the soil of some region, water body or deposit) and carries out such as Dissolved Organic Matter
The bioid of (Dissolved Organic Matter, DOM) component analysis, substance source analysis and material transport transformation rule
Learn characteristic research.
Wherein, it during treating test substance progress DOM component detection using three-dimensional fluorescence spectrum technology, needs to grasp
Make personnel determined from the fluorescence data of the material sample (such as water sample or soil sample) to be test every DOM component (for example,
Fulvic acid, humic acid, aromatic polymer and carbohydrate) fluorescence spectrum peak distributing position.And industry mainstream is usual at present
It is realized using method or parallel transport is manually determined and operation is determined to fluorescence spectrum peak distributing position, wherein people
There is the disadvantages of time-consuming, human resources consumption is big, intuitive is not strong and precision is not high, parallel factor point in work manually identifying method
Analysis method needs operator to have extremely strong professional knowledge, and ordinary person is difficult to realize fluorescence spectrum based on parallel transport
Peak distributing position determines operation, and whole generalization and intuitive be not strong.
Summary of the invention
In order to overcome above-mentioned deficiency in the prior art, the application's is designed to provide a kind of quickly screening fluorescence spectrum
The operation difficulty of the method and device of peak value batch data, the method is low, and generalization is strong, can quickly and accurately determine wait divide
The fluorescence spectrum peak distributing position of items DOM component in sample is analysed, and corresponding effect of visualization is provided, it is whole intuitively to realize
A fluorescence spectrum peak screening process.
For method, the embodiment of the present application provides a kind of method of quickly screening fluorescence spectrum peak value batch data, answers
For the terminal device including display component, which comprises
The three-dimensional fluorescence spectrum data of at least one sample to be analyzed are obtained, wherein each sample to be analyzed is one group three corresponding
Tie up fluorescence data, spectroscopic data described in every group include corresponding sample to be analyzed in three-dimensional fluorescence spectrum test process extremely
Fluorescence emission wavelengths numerical value, excitation wavelength numerical value and florescent intensity value corresponding to a few fluorescence spectrum peak;
Obtain fluorescence emission wavelengths range and excitation wavelength model corresponding at least one Dissolved Organic Matter DOM component
It encloses;
In the current display picture of the display component the corresponding each fluorescence spectrum peak of the spectroscopic data described in every group into
The calibration of row location point, and borderline region delimitation is carried out at least one of described DOM component in the display picture;
Whether it is distributed in borderline region corresponding to each DOM component at least one described DOM component of detection glimmering
The location point of light spectrum peak, and determine each DOM component at least one of described DOM component in corresponding sides according to testing result
Matched target fluorescent spectrum peak in battery limit (BL) domain;
To the fluorescence of the target fluorescent spectrum peak of each DOM components do match at least one of described in obtaining in DOM component
Launch wavelength numerical value, excitation wavelength numerical value and florescent intensity value carry out data summarization, obtain corresponding fluorescence spectrum peak sieve
Select result.
Optionally, in the embodiment of the present application, there is one with fluorescence emission wavelengths and exciting light creation in above-mentioned display picture
Displaing coordinate system of the wavelength as coordinate variable,
In the current display picture of the display component the corresponding each fluorescence spectrum peak of the spectroscopic data described in every group into
Row location point demarcate the step of include:
For each fluorescence spectrum peak, according to the fluorescent emission of the fluorescence spectrum peak in corresponding three-dimensional fluorescence data
Wavelength values and excitation wavelength numerical value obtain the fluorescence spectrum peak corresponding position coordinates in the displaing coordinate system;
The position at the fluorescence spectrum peak is drawn out in the displaing coordinate system according to the position coordinates at the fluorescence spectrum peak
Point.
Optionally, in the embodiment of the present application, it is above-mentioned in the display picture at least one of described DOM component into
The step of row bound regional assignment includes:
For each DOM component at least one of described DOM component, sent out according to the fluorescence of the DOM component got
Wave-length coverage and excitation wavelength range are penetrated, the DOM component corresponding each extreme coordinates in the displaing coordinate system are obtained;
It is corresponding that the DOM component is drawn out in the displaing coordinate system according to the corresponding each extreme coordinates of the DOM component
Borderline region.
Optionally, in the embodiment of the present application, above-mentioned displaing coordinate system is using fluorescence emission wavelengths as abscissa and to swash
Emission wavelength is the coordinate system of ordinate, or using a length of abscissa of excitation light wave and using fluorescence emission wavelengths as the coordinate of ordinate
System.
Optionally, in the embodiment of the present application, every in above-mentioned at least one described DOM component determining according to testing result
DOM component includes: the step of matched target fluorescent spectrum peak in corresponding borderline region
For each DOM component at least one of described DOM component, if the testing result of the DOM component is corresponding sides
Without location point in battery limit (BL) domain, then the target fluorescent spectrum peak of the corresponding DOM component of the borderline region is sky;
If the testing result of the DOM component is that there are a location points in corresponding borderline region, with the location point, institute is right
The fluorescence spectrum peak answered, the target fluorescent spectrum peak as the corresponding DOM component of the borderline region;
If the testing result of the DOM component is that there are at least two location points in corresponding borderline region, from the frontier district
In the corresponding fluorescence spectrum peak of all location points in domain, the maximum fluorescence spectrum peak of selection florescent intensity value is used as should
The target fluorescent spectrum peak of the corresponding DOM component of borderline region.
For device, the embodiment of the present application provides a kind of device of quickly screening fluorescence spectrum peak value batch data, answers
For the terminal device including display component, described device includes:
Spectroscopic data obtains module, for obtaining the three-dimensional fluorescence spectrum data of at least one sample to be analyzed, wherein often
A corresponding one group of three-dimensional fluorescence spectrum data of sample to be analyzed, spectroscopic data described in every group include corresponding sample to be analyzed in three-dimensional
Fluorescence emission wavelengths numerical value, excitation wavelength numerical value corresponding at least one fluorescence spectrum peak in fluorescence spectrum test process
And florescent intensity value;
Component parameter obtains module, for obtaining fluorescent emission corresponding at least one Dissolved Organic Matter DOM component
Wave-length coverage and excitation wavelength range;
Image Rendering module is shown, for the spectroscopic data described in every group in the current display picture of the display component
Corresponding each fluorescence spectrum peak carries out location point calibration, and in the display picture at least one described DOM component into
Row bound regional assignment;
Peak detection determining module, for detecting side corresponding to each DOM component at least one of described DOM component
The location point at fluorescence spectrum peak whether is distributed in battery limit (BL) domain, and is determined at least one of described DOM component according to testing result
Each DOM component in corresponding borderline region matched target fluorescent spectrum peak;
The selection result output module, for each DOM components do match at least one of described in obtaining in DOM component
Fluorescence emission wavelengths numerical value, excitation wavelength numerical value and the florescent intensity value of target fluorescent spectrum peak carry out data summarization, obtain
To corresponding fluorescence spectrum peak the selection result.
Optionally, in the embodiment of the present application, there is one with fluorescence emission wavelengths and exciting light creation in above-mentioned display picture
Displaing coordinate system of the wavelength as coordinate variable, the display Image Rendering module are specifically used for:
For each fluorescence spectrum peak, according to the fluorescent emission of the fluorescence spectrum peak in corresponding three-dimensional fluorescence data
Wavelength values and excitation wavelength numerical value obtain the fluorescence spectrum peak corresponding position coordinates in the displaing coordinate system;
The position at the fluorescence spectrum peak is drawn out in the displaing coordinate system according to the position coordinates at the fluorescence spectrum peak
Point.
Optionally, in the embodiment of the present application, above-mentioned display Image Rendering module also particularly useful for:
For each DOM component at least one of described DOM component, sent out according to the fluorescence of the DOM component got
Wave-length coverage and excitation wavelength range are penetrated, the DOM component corresponding each extreme coordinates in the displaing coordinate system are obtained;
It is corresponding that the DOM component is drawn out in the displaing coordinate system according to the corresponding each extreme coordinates of the DOM component
Borderline region.
Optionally, in the embodiment of the present application, above-mentioned displaing coordinate system is using fluorescence emission wavelengths as abscissa and to swash
Emission wavelength is the coordinate system of ordinate, or using a length of abscissa of excitation light wave and using fluorescence emission wavelengths as the coordinate of ordinate
System.
Optionally, in the embodiment of the present application, above-mentioned peak detection determining module is specifically used for:
For each DOM component at least one of described DOM component, if the testing result of the DOM component is corresponding sides
Without location point in battery limit (BL) domain, then the target fluorescent spectrum peak of the corresponding DOM component of the borderline region is sky;
If the testing result of the DOM component is that there are a location points in corresponding borderline region, with the location point, institute is right
The fluorescence spectrum peak answered, the target fluorescent spectrum peak as the corresponding DOM component of the borderline region;
If the testing result of the DOM component is that there are at least two location points in corresponding borderline region, from the frontier district
In the corresponding fluorescence spectrum peak of all location points in domain, the maximum fluorescence spectrum peak of selection florescent intensity value is used as should
The target fluorescent spectrum peak of the corresponding DOM component of borderline region.
In terms of existing technologies, a kind of quickly screening fluorescence spectrum peak value batch data provided by the embodiments of the present application
Method and device have the advantages that the operation difficulty of the method is low, generalization is strong, can quickly and accurately determine
The fluorescence spectrum peak distributing position of items DOM component in sample to be analyzed, and corresponding effect of visualization is provided, with intuitively real
Now entire fluorescence spectrum peak screening process.The method is applied to the terminal device including display component, and the method is obtaining
To the three-dimensional fluorescence spectrum data of at least one sample to be analyzed, and fluorescent emission wave corresponding at least one DOM component
It, can be corresponding to every group of spectroscopic data each in the current display picture of the display component after long range and excitation wavelength range
Fluorescence spectrum peak carries out location point calibration, and to each corresponding borderline region of DOM component at least one of described DOM component
It delimited, to show the location point distribution situation at each fluorescence spectrum peak and the side of items DOM component by the display component
Boundary's Regional Distribution of Registered reaches the visualization of fluorescence spectrum peak distributing position of every DOM component at all samples to be analyzed
Effect.Meanwhile the method can also accordingly detect boundary corresponding to each DOM component at least one of described DOM component
The location point at fluorescence spectrum peak whether is distributed in region, and determines each DOM component in corresponding frontier district according to testing result
Matched target fluorescent spectrum peak in domain, with the fluorescence emission wavelengths numerical value to obtained all target fluorescent spectrum peaks, excitation
Optical wavelength numerical value and florescent intensity value carry out data summarization, corresponding fluorescence spectrum peak the selection result are exported, so that it is guaranteed that institute
The method of stating has the characteristics that operation difficulty is low and generalization is strong, and can quickly and accurately determine each in all samples to be analyzed
The fluorescence spectrum peak distributing position of item DOM component, it is strong to be provided for the research of the biochemical characteristic of sample of material to be analyzed
Help.
To enable the above objects, features, and advantages of the application to be clearer and more comprehensible, the application preferred embodiment is cited below particularly,
And cooperate appended attached drawing, it is described in detail below.
Detailed description of the invention
Technical solution in ord to more clearly illustrate embodiments of the present application, below will be to needed in the embodiment attached
Figure is briefly described, it should be understood that the following drawings illustrates only some embodiments of the application, therefore is not construed as pair
The restriction of the claim of this application protection scope, for those of ordinary skill in the art, what is do not made the creative labor
Under the premise of, it can also be obtained according to these attached drawings other relevant attached drawings.
Fig. 1 is the block diagram of terminal device provided by the embodiments of the present application.
Fig. 2 is that a kind of process of the method for quickly screening fluorescence spectrum peak value batch data provided by the embodiments of the present application is shown
It is intended to.
Fig. 3 is the flow diagram for the sub-step that step S240 includes in Fig. 2.
Fig. 4 is the block diagram of fluorescence spectrum peak provided by the embodiments of the present application screening plant.
Icon: 10- terminal device;11- memory;12- processor;13- communication unit;14- display component;100- fluorescence
Spectrum peak screening plant;110- spectroscopic data obtains module;120- component parameter obtains module;130- shows Image Rendering mould
Block;140- peak detection determining module;150- the selection result output module.
Specific embodiment
To keep the purposes, technical schemes and advantages of the embodiment of the present application clearer, below in conjunction with the embodiment of the present application
In attached drawing, the technical scheme in the embodiment of the application is clearly and completely described, it is clear that described embodiment is
Some embodiments of the present application, instead of all the embodiments.The application being usually described and illustrated herein in the accompanying drawings is implemented
The component of example can be arranged and be designed with a variety of different configurations.
Therefore, the detailed description of the embodiments herein provided in the accompanying drawings is not intended to limit below claimed
Scope of the present application, but be merely representative of the selected embodiment of the application.Based on the embodiment in the application, this field is common
Technical staff's every other embodiment obtained without creative efforts belongs to the model of the application protection
It encloses.
It should also be noted that similar label and letter indicate similar terms in following attached drawing, therefore, once a certain Xiang Yi
It is defined in a attached drawing, does not then need that it is further defined and explained in subsequent attached drawing.
In the description of the present application, it should be noted that unless otherwise clearly defined and limited, term " setting ", " peace
Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally
Connection;It can be mechanical connection, be also possible to be electrically connected;Can be directly connected, can also indirectly connected through an intermediary,
It can be the connection inside two elements.For the ordinary skill in the art, above-mentioned art can be understood with concrete condition
The concrete meaning of language in this application.
With reference to the accompanying drawing, it elaborates to some embodiments of the application.In the absence of conflict, following
Feature in embodiment and embodiment can be combined with each other.
Fig. 1 is please referred to, is the block diagram of terminal device 10 provided by the embodiments of the present application.In the embodiment of the present application
In, the terminal device 10 can be used for determining items DOM component matched fluorescence spectrum peak distributing position at sample to be analyzed,
To accelerate the DOM component analysis flow of research of sample to be analyzed.Wherein the sample to be analyzed is to need to carry out biochemistry spy
The sample of the substance of Journal of Sex Research, the substance may be, but not limited to, soil, water body and the deposit etc. in some area;It is described
Every DOM component may include class humic acid, visual field class fulvic acid, ultra-violet (UV) band class fulvic acid, visual field class tryptophan, ultraviolet
Area's class tryptophan, visual field class tyrosine and ultra-violet (UV) band class tyrosine etc.;The terminal device 10 may be, but not limited to, intelligence
It can mobile phone, PC (personal computer, PC), tablet computer, personal digital assistant (personal digital
Assistant, PDA), mobile internet surfing equipment (mobile Internet device, MID) etc..
In the present embodiment, the terminal device 10 includes fluorescence spectrum peak screening plant 100, memory 11, processor
12, communication unit 13 and display component 14.The memory 11, processor 12, communication unit 13 and 14 each yuan of display component
Part is directly or indirectly electrically connected between each other, to realize the transmission or interaction of data.For example, these elements between each other may be used
It is realized and is electrically connected by one or more communication bus or signal wire.
In the present embodiment, the memory 11 can be used for storing the three-dimensional fluorescence spectrum number of at least one sample to be analyzed
According to, wherein the corresponding one group of three-dimensional fluorescence spectrum data of each sample to be analyzed, spectroscopic data described in every group include it is corresponding to
Fluorescence emission wavelengths numerical value corresponding at least one the fluorescence spectrum peak of analysis sample in three-dimensional fluorescence spectrum test process,
Excitation wavelength numerical value and florescent intensity value.The excitation wavelength numerical value is glimmering for indicating to carry out for the sample to be analyzed
The wavelength values of light used excitation beam when exciting, the fluorescence emission wavelengths numerical value is for indicating that the substance to be analyzed exists
The wavelength values for the fluorescent light beam accordingly launched under the excitation of excitation beam, the florescent intensity value for indicate by
The brightness number for the fluorescent light beam that the substance to be analyzed is launched, the fluorescence spectrum peak is for indicating the substance to be analyzed at certain
Launch the shape for the fluorescent light beam that brightness number is some peak value under the excitation of the excitation beam of a excitation wavelength numerical value
State.Wherein, at least one described sample to be analyzed can be what substance to be analyzed was distributed according to different location and/or different time
At least one sample, each fluorescence spectrum peak in every group of spectroscopic data compared to other fluorescence spectrum peaks exist for indicate from
The signature identification of body identity information, the signature identification can be at least one of number, letter, character, Chinese or
Multiple combinations.
The memory 11 can also be used in the fluorescence emission wavelengths range and excitation wavelength of at least one of storage DOM component
Range, the excitation wavelength range are used to indicate the number of wavelengths of the DOM component required excitation beam when emitting light beam
It is worth range, the fluorescence emission wavelengths range is used to indicate the DOM component in the number of wavelengths of light beam for being excited and being launched
It is worth range.For example, the excitation wavelength of class humic acid may range from 345nm~450nm, or 330~460nm, tool
The numberical range of body can carry out different settings according to demand;The fluorescence emission wavelengths of class humic acid may range from 430nm~
530nm, or 400~550nm, specific numberical range can carry out different settings according to demand.Wherein, same item
May exist corresponding numerical value constraint condition between the fluorescence emission wavelengths range and excitation wavelength range of DOM component, for example,
Between certain section of fluorescence emission wavelengths and certain section of excitation wavelength of some DOM component and value is not more than some wavelength values, should
Between certain section of fluorescence emission wavelengths and certain section of excitation wavelength of DOM component and value is not less than some wavelength values or the DOM
Any numerical value constraint condition is not present between all fluorescence emission wavelengths and all excitation wavelengths of component, specific numerical value is about
Beam situation can carry out different configurations according to demand.
In the present embodiment, the memory 11 can also store program, and the processor 12 is executed instruction receiving
Afterwards, described program can correspondingly be executed.
In the present embodiment, the processor 12 can be a kind of IC chip of processing capacity with signal.
The processor 12 can be general processor, including central processing unit (Central Processing Unit, CPU), figure
Processor (Graphics Processing Unit, GPU), network processing unit (Network Processor, NP) etc..It is general
Processor can be microprocessor, and perhaps the processor is also possible to any conventional processor etc. and may be implemented or execute sheet
Apply for disclosed each method, step and the logic diagram in embodiment.
In the present embodiment, the communication unit 13 is set for establishing the terminal device 10 by network with other electronics
Communication connection between standby, and pass through the network sending and receiving data.For example, the terminal device 10 passes through the communication unit 13
Obtain the three-dimensional fluorescence spectrum data of some sample to be analyzed from three-dimensional fluorescence spectrum instrument, and by the communication unit 13 to
Other electronic equipments export the fluorescence spectrum peak the selection result obtained by the terminal device 10.
In the present embodiment, the display component 14 include display screen, the display component 14 by the display screen into
Row picture is shown, so that the operator of the terminal device 10 can carry out data observation by the display screen.
In the present embodiment, fluorescence spectrum peak screening plant 100 includes that at least one can be with software or firmware
Form is stored in the memory 11 or is solidificated in the software function module in the operating system of the terminal device 10.It is described
Processor 12 can be used for executing the executable module that the memory 11 stores, such as fluorescence spectrum peak screening plant 100
Included software function module and computer program etc..The terminal device 10 passes through fluorescence spectrum peak screening plant
100 carry out operation difficulties are low, generalization is strong, time-consuming short and high accurancy and precision every DOM component for substance to be analyzed it is glimmering
Light spectrum peak distributing position determines operation, and provides corresponding effect of visualization, intuitively to realize entire fluorescence spectrum peak
Screening process, to provide strong help for the research of the biochemical characteristic of sample of material to be analyzed.
It is understood that block diagram shown in FIG. 1 is only a kind of structure composition schematic diagram of terminal device 10, the terminal
Equipment 10 may also include than shown in Fig. 1 more perhaps less component or with the configuration different from shown in Fig. 1.In Fig. 1
Shown in each component can using hardware, software, or its combination realize.
It referring to figure 2., is a kind of method of quickly screening fluorescence spectrum peak value batch data provided by the embodiments of the present application
Flow diagram.In the embodiment of the present application, the method is applied to the above-mentioned terminal device 10 including display component 14,
The detailed process and step of method shown in Fig. 2 are described in detail below.
Step S210 obtains the three-dimensional fluorescence spectrum data of at least one sample to be analyzed.
In the present embodiment, the terminal device 10 can obtain target point by communication unit 13 from other electronic equipments
The three-dimensional fluorescence spectrum data for analysing at least one sample to be analyzed of substance, can also be by the memory 11 itself installed
The middle mode for carrying out data search obtains the three-dimensional fluorescence spectrum data of at least one sample to be analyzed.Wherein, the end
The corresponding sample to be analyzed of every group of three-dimensional fluorescence spectrum data that end equipment 10 is got, the three-dimensional fluorescence spectrum data packet
Include the fluorescent emission at corresponding sample to be analyzed at least one fluorescence spectrum peak corresponding in three-dimensional fluorescence spectrum test process
Wavelength values, excitation wavelength numerical value and florescent intensity value.
Step S220 obtains fluorescence emission wavelengths range and excitation wavelength range corresponding at least one DOM component.
In the present embodiment, the terminal device 10 can be by way of providing external data equipment interface and external data
Input equipment is electrically connected, and is directed to certain to receive user by what the external data input equipment was inputted to the terminal device 10
The fluorescence emission wavelengths range and excitation wavelength range of item DOM component, or reception user are directed to and are stored by the terminal device 10
A certain DOM component fluorescence emission wavelengths range and excitation wavelength range parameter modify operation.The terminal device 10
Fluorescence emission wavelengths range corresponding to items DOM component can also be directly obtained from the memory 11 that itself is installed and is swashed
Emission wavelength range, so that at least one the DOM component for obtaining participating in entire fluorescence spectrum peak screening process is corresponding
Fluorescence emission wavelengths range and excitation wavelength range.
Step S230, the corresponding each fluorescence light of the spectroscopic data described in every group in the current display picture of display component 14
Spectral peak carries out location point calibration, and carries out borderline region delimitation at least one of described DOM component in the display picture.
In the present embodiment, the terminal device 10 is obtaining all three-dimensional fluorescence spectrum data, and participates in glimmering
After the corresponding fluorescence emission wavelengths range of all DOM components and excitation wavelength range of light spectrum peak screening process, meeting
By the position for calibrating each fluorescence spectrum peak in every group of spectroscopic data on the current display picture of the display component 14
It sets a little, and participates in the mode of at least one described corresponding borderline region of DOM component of fluorescence spectrum peak screening process, make institute
It states display component 14 and shows the location point distribution situation at each fluorescence spectrum peak and the borderline region distribution situation of items DOM component,
It is all to be analyzed what is currently got to ensure that user can be intuitively understood from the display component 14 to every DOM component
Fluorescence spectrum peak distributing position at sample, reaches corresponding effect of visualization.
In the present embodiment, there is one using fluorescence emission wavelengths and excitation wavelength as coordinate creation in the display picture
The displaing coordinate system of variable, the terminal device 10 realize the location point calibration at each fluorescence spectrum peak in the displaing coordinate system
The borderline region of operation and items DOM component delimit operation.Wherein, the displaing coordinate system can be is with fluorescence emission wavelengths
Abscissa and with the coordinate system of a length of ordinate of excitation light wave, is also possible to a length of abscissa of excitation light wave and with fluorescent emission
Wavelength is the coordinate system of ordinate.In an embodiment of the present embodiment, the displaing coordinate system is preferably sent out with fluorescence
The a length of abscissa of ejected wave and with the coordinate system of a length of ordinate of excitation light wave.
Optionally, the corresponding each fluorescence spectrum of the spectroscopic data described in every group in the current display picture of display component 14
Peak carry out location point calibration the step of include:
For each fluorescence spectrum peak, according to the fluorescent emission of the fluorescence spectrum peak in corresponding three-dimensional fluorescence data
Wavelength values and excitation wavelength numerical value obtain the fluorescence spectrum peak corresponding position coordinates in the displaing coordinate system;
The position at the fluorescence spectrum peak is drawn out in the displaing coordinate system according to the position coordinates at the fluorescence spectrum peak
Point.
Wherein, corresponding position coordinates must be corresponding with the coordinate system in the displaing coordinate system at each fluorescence spectrum peak
Matching.The terminal device 10 can use each corresponding signature identification pair in fluorescence spectrum peak in the displaing coordinate system
The mode that the location point at the fluorescence spectrum peak is labeled, to distinguish each fluorescence spectrum peak in the displaing coordinate system;It is described
Terminal device 10 can also the lines in the displaing coordinate system using different colours draw the position at corresponding fluorescence spectrum peak
The mode of point, to distinguish each fluorescence spectrum peak in the displaing coordinate system;The terminal device 10 can also be sat in the display
In mark system in such a way that the lines of different thicknesses draw the location point at corresponding fluorescence spectrum peak, to distinguish the displaing coordinate
Each fluorescence spectrum peak in system.
Optionally, the step of carrying out borderline region delimitation at least one of described DOM component in the display picture
Include:
For each DOM component at least one of described DOM component, sent out according to the fluorescence of the DOM component got
Wave-length coverage and excitation wavelength range are penetrated, the DOM component corresponding each extreme coordinates in the displaing coordinate system are obtained;
It is corresponding that the DOM component is drawn out in the displaing coordinate system according to the corresponding each extreme coordinates of the DOM component
Borderline region.
Wherein, for the terminal device 10 before obtaining the corresponding all extreme coordinates of a certain DOM component, needing to obtain should
The fluorescence emission wavelengths range and excitation wavelength range and the fluorescence emission wavelengths range and the excitation of DOM component
Numerical value constraint condition that may be present between optical wavelength range, thus according to the fluorescence emission wavelengths range, the exciting light
Wave-length coverage and the numerical value constraint condition determine that the corresponding borderline region of this DOM component is rectangular area or triangle
Shape region also or other polygonal regions then accordingly obtains each extreme coordinates of the borderline region.For example, a certain DOM group
The fluorescence emission wavelengths range divided is 345nm~450nm, and excitation wavelength range is 430nm~530nm, and numerical value at this time is about
When beam condition is that excitation wavelength is not more than the fluorescence emission wavelengths and 90nm, which is five
Side shape region, correspondence is obtained the DOM component corresponding frontier district in the displaing coordinate system by the terminal device 10 at this time
Each extreme coordinates in domain, and the corresponding borderline region of DOM component is drawn out in the displaing coordinate system;A certain DOM component
Fluorescence emission wavelengths range be 350nm~450nm, excitation wavelength range be 470nm~530nm, the numerical value of the DOM component
When constraint condition is empty, which is rectangular area, and the terminal device 10 obtains correspondence at this time
The DOM component corresponding four extreme coordinates in the displaing coordinate system, and correspondingly drawn in the displaing coordinate system
The corresponding borderline region of DOM component out.
In addition, the terminal device 10 can use the corresponding signature identification of items DOM component in the displaing coordinate system
To the mode that the corresponding borderline region of DOM component is labeled, to distinguish each borderline region in the displaing coordinate system;Institute
State terminal device 10 can also by the way of the lines in the displaing coordinate system using different colours draw corresponding borderline region,
To distinguish each borderline region in the displaing coordinate system;The terminal device 10 can also be in the displaing coordinate system using not
The mode of corresponding borderline region is drawn, with the lines of thickness to distinguish each borderline region in the displaing coordinate system.
Step S240, in borderline region corresponding to each DOM component at least one of the described DOM component of detection whether
The location point at fluorescence spectrum peak is distributed with, and determines each DOM component at least one of described DOM component according to testing result
The matched target fluorescent spectrum peak in corresponding borderline region.
In the present embodiment, the terminal device 10 can be by position coordinates corresponding to the location point at each fluorescence spectrum peak
It carries out coordinate value with the area coordinate coverage area of each borderline region to compare, and the cross of the location point at some fluorescence spectrum peak
When coordinate value and ordinate value are each fallen in the area coordinate coverage area of some borderline region, the fluorescence spectrum peak is determined
Location point is distributed in the borderline region.Then the terminal device 10 is got corresponding at least one of described DOM component
Each DOM component corresponding to for indicating the detection knot of the location point in corresponding borderline region with the presence or absence of fluorescence spectrum peak
Fruit, and determine the DOM component described at least one according to the testing result of each DOM component at least one of described DOM component
Matched target fluorescent spectrum peak at a sample to be analyzed.
It referring to figure 3., is the flow diagram for the sub-step that step S240 includes in Fig. 2.In the present embodiment, the step
Each DOM component determined at least one of described DOM component according to testing result in rapid S240 is in corresponding borderline region
The step of matched target fluorescent spectrum peak may include sub-step S241, sub-step S242 and sub-step S243.
Sub-step S241, for each DOM component at least one of described DOM component, if the detection knot of the DOM component
Fruit is in corresponding borderline region without location point, then the target fluorescent spectrum peak of the corresponding DOM component of the borderline region is sky.
Sub-step S242, if the testing result of the DOM component is that there are a location points in corresponding borderline region, with this
Fluorescence spectrum peak corresponding to location point, the target fluorescent spectrum peak as the corresponding DOM component of the borderline region.
Sub-step S243, if the testing result of the DOM component is in corresponding borderline region there are at least two location points,
From the corresponding fluorescence spectrum peak of all location points in the borderline region, the maximum fluorescence light of florescent intensity value is chosen
Target fluorescent spectrum peak of the spectral peak as the corresponding DOM component of the borderline region.
Wherein, if some target fluorescent spectrum peak is empty, show corresponding DOM component at the substance to be analyzed without reality
The matched fluorescence spectrum peak of matter, at this time the fluorescence emission wavelengths numerical value of the target fluorescent spectrum peak, excitation wavelength numerical value and glimmering
Optical power values are sky.
Step S250, to the target fluorescent spectrum of each DOM components do match at least one of described in obtaining in DOM component
Fluorescence emission wavelengths numerical value, excitation wavelength numerical value and the florescent intensity value at peak carry out data summarization, obtain corresponding fluorescence
Spectrum peak the selection result.
In the present embodiment, the terminal device 10 exists in each the DOM component obtained at least one of described DOM component
It, can be to each target fluorescent spectrum peak and at least one of described at all samples to be analyzed after matched target fluorescent spectrum peak
The fluorescence emission wavelengths numerical value of corresponding relationship, each target fluorescent spectrum peak in DOM component between items DOM component, excitation
The signature identification of optical wavelength numerical value and florescent intensity value and each target fluorescent spectrum peak carry out data summarization, obtain with
Word form or Excel form carry out the fluorescence spectrum peak the selection result of data record, so that it is guaranteed that the method has operation
The feature that difficulty is low and generalization is strong, and can quickly and accurately determine the glimmering of items DOM component in all samples to be analyzed
Light spectrum peak distributing position, to provide strong help for the research of the biochemical characteristic of sample of material to be analyzed.
It referring to figure 4., is the block diagram of fluorescence spectrum peak provided by the embodiments of the present application screening plant 100.At this
Apply in embodiment, fluorescence spectrum peak screening plant 100 includes that spectroscopic data obtains module 110, component parameter obtains mould
Block 120, display Image Rendering module 130, peak detection determining module 140 and the selection result output module 150.Wherein, described
Fluorescence spectrum peak screening plant 100 is a kind of quickly screening fluorescence spectrum peak value batch data provided by the embodiments of the present application
Device, method corresponding of fluorescence spectrum peak screening plant 100 and above-mentioned quick screening fluorescence spectrum peak value batch data
Match.
The spectroscopic data obtains module 110, for obtaining the three-dimensional fluorescence spectrum data of at least one sample to be analyzed,
The wherein corresponding one group of three-dimensional fluorescence spectrum data of each sample to be analyzed, spectroscopic data described in every group include corresponding sample to be analyzed
Fluorescence emission wavelengths numerical value, excitation light wave corresponding at least one fluorescence spectrum peak in three-dimensional fluorescence spectrum test process
Long numerical value and florescent intensity value.
In the present embodiment, the spectroscopic data, which obtains module 110, can execute the step S210 in Fig. 2, specifically retouch
Stating can refer to above to the detailed description of step S210.
The component parameter obtains module 120, for obtaining fluorescence emission wavelengths model corresponding at least one DOM component
It encloses and excitation wavelength range.
In the present embodiment, the component parameter, which obtains module 120, can execute the step S220 in Fig. 2, specifically retouch
Stating can refer to above to the detailed description of step S220.
The display Image Rendering module 130, for the light described in every group in the current display picture of display component 14
The corresponding each fluorescence spectrum peak of modal data carries out location point calibration, and at least one of described DOM in the display picture
Component carries out borderline region delimitation.
In the present embodiment, there is one using fluorescence emission wavelengths and excitation wavelength as coordinate creation in the display picture
The displaing coordinate system of variable, the display Image Rendering module 130 can execute the step S230 in Fig. 2, and specific description can
Referring to above to the detailed description of step S230.
The peak detection determining module 140, for detecting each DOM component institute at least one of described DOM component
The location point at fluorescence spectrum peak whether is distributed in corresponding borderline region, and determines according to testing result at least one of described
Each DOM component in DOM component is corresponding to matched target fluorescent spectrum peak in borderline region.
In the present embodiment, the peak detection determining module 140 can execute in step S240 and Fig. 3 in Fig. 2
Sub-step S241, sub-step S242 and sub-step S243, specific description can refer to above to step S240, sub-step
The detailed description of S241, sub-step S242 and sub-step S243.
The selection result output module 150, for each DOM group at least one of described in obtaining in DOM component
The fluorescence emission wavelengths numerical value, excitation wavelength numerical value and florescent intensity value of matched target fluorescent spectrum peak is divided to carry out data
Summarize, obtains corresponding fluorescence spectrum peak the selection result.
In the present embodiment, the composition parameter, which obtains module 120, can execute the step S250 in Fig. 2, specifically retouch
Stating can refer to above to the detailed description of step S250.
In conclusion a kind of quickly screening fluorescence spectrum peak value batch data provided by the embodiments of the present application method and
In device, the operation difficulty of the method is low, and generalization is strong, can quickly and accurately determine items DOM group in sample to be analyzed
The fluorescence spectrum peak distributing position divided, and corresponding effect of visualization is provided, intuitively to realize entire fluorescence spectrum peak screening
Process.The method is applied to the terminal device including display component, and the method is getting at least one sample to be analyzed
Three-dimensional fluorescence spectrum data, and at least one of fluorescence emission wavelengths range and excitation wavelength model corresponding to DOM component
After enclosing, location point can be carried out in each fluorescence spectrum peak corresponding to every group of spectroscopic data in the current display picture of the display component
Calibration, and each corresponding borderline region of DOM component at least one of described DOM component delimited, by described
Display component shows the location point distribution situation at each fluorescence spectrum peak and the borderline region distribution situation of items DOM component, reaches
The effect of visualization of fluorescence spectrum peak distributing position of every DOM component at all samples to be analyzed.Meanwhile the method is also
It can accordingly detect in borderline region corresponding to each DOM component at least one of described DOM component and whether fluorescence be distributed with
The location point of spectrum peak, and each DOM component matched target fluorescent light in corresponding borderline region is determined according to testing result
Spectral peak, with fluorescence emission wavelengths numerical value, excitation wavelength numerical value and the fluorescence intensity to obtained all target fluorescent spectrum peaks
Numerical value carries out data summarization, exports corresponding fluorescence spectrum peak the selection result, so that it is guaranteed that the method has operation difficulty low
And the feature that generalization is strong, and can quickly and accurately determine the fluorescence spectrum of items DOM component in all samples to be analyzed
Peak distributing position, to provide strong help for the research of the biochemical characteristic of sample of material to be analyzed.
The foregoing is merely preferred embodiment of the present application, are not intended to limit this application, for the skill of this field
For art personnel, various changes and changes are possible in this application.Within the spirit and principles of this application, made any to repair
Change, equivalent replacement, improvement etc., should be included within the scope of protection of this application.
Claims (10)
1. a kind of method of quickly screening fluorescence spectrum peak value batch data, which is characterized in that applied to including display component
Terminal device, which comprises
The three-dimensional fluorescence spectrum data of at least one sample to be analyzed are obtained, wherein the corresponding one group of three-dimensional of each sample to be analyzed is glimmering
Light spectroscopic data, spectroscopic data described in every group include at least one of corresponding sample to be analyzed in three-dimensional fluorescence spectrum test process
Fluorescence emission wavelengths numerical value corresponding to a fluorescence spectrum peak, excitation wavelength numerical value and florescent intensity value;
Obtain fluorescence emission wavelengths range and excitation wavelength range corresponding at least one Dissolved Organic Matter DOM component;
The corresponding each fluorescence spectrum peak of the spectroscopic data described in every group carries out position in the current display picture of the display component
A calibration is set, and carries out borderline region delimitation at least one of described DOM component in the display picture;
Fluorescence light whether is distributed in borderline region corresponding to each DOM component at least one described DOM component of detection
The location point of spectral peak, and determine each DOM component at least one of described DOM component in corresponding frontier district according to testing result
Matched target fluorescent spectrum peak in domain;
To the fluorescent emission of the target fluorescent spectrum peak of each DOM components do match at least one of described in obtaining in DOM component
Wavelength values, excitation wavelength numerical value and florescent intensity value carry out data summarization, obtain corresponding fluorescence spectrum peak screening knot
Fruit.
2. the method according to claim 1, wherein there is one with fluorescence emission wavelengths creation in the display picture
And displaing coordinate system of the excitation wavelength as coordinate variable, in the current display picture of the display component described in every group
The step of corresponding each fluorescence spectrum peak of spectroscopic data carries out location point calibration include:
For each fluorescence spectrum peak, according to the fluorescence emission wavelengths of the fluorescence spectrum peak in corresponding three-dimensional fluorescence data
Numerical value and excitation wavelength numerical value obtain the fluorescence spectrum peak corresponding position coordinates in the displaing coordinate system;
The location point at the fluorescence spectrum peak is drawn out in the displaing coordinate system according to the position coordinates at the fluorescence spectrum peak.
3. according to the method described in claim 2, it is characterized in that, at least one of described DOM in the display picture
Component carry out borderline region delimitation the step of include:
For each DOM component at least one of described DOM component, according to the fluorescent emission wave of the DOM component got
Long range and excitation wavelength range obtain the DOM component corresponding each extreme coordinates in the displaing coordinate system;
The corresponding boundary of DOM component is drawn out in the displaing coordinate system according to the corresponding each extreme coordinates of the DOM component
Region.
4. according to the method described in claim 3, it is characterized in that, the displaing coordinate system is with fluorescence emission wavelengths for horizontal seat
Mark and with the coordinate system of a length of ordinate of excitation light wave, or with a length of abscissa of excitation light wave and with fluorescence emission wavelengths be vertical sit
Target coordinate system.
5. method described in any one of -4 according to claim 1, which is characterized in that it is described according to testing result determine described in
At least one of each DOM component in DOM component include: the step of matched target fluorescent spectrum peak in corresponding borderline region
For each DOM component at least one of described DOM component, if the testing result of the DOM component is corresponding frontier district
Without location point in domain, then the target fluorescent spectrum peak of the corresponding DOM component of the borderline region is sky;
If the testing result of the DOM component is that there are a location points in corresponding borderline region, corresponding to the location point
Fluorescence spectrum peak, the target fluorescent spectrum peak as the corresponding DOM component of the borderline region;
If the testing result of the DOM component is that there are at least two location points in corresponding borderline region, out of this borderline region
The corresponding fluorescence spectrum peak of all location points in, choose the maximum fluorescence spectrum peak of florescent intensity value as the boundary
The target fluorescent spectrum peak of the corresponding DOM component in region.
6. a kind of device of quickly screening fluorescence spectrum peak value batch data, which is characterized in that applied to including display component
Terminal device, described device include:
Spectroscopic data obtain module, for obtaining the three-dimensional fluorescence spectrum data of at least one sample to be analyzed, wherein each to
The corresponding one group of three-dimensional fluorescence spectrum data of sample are analyzed, spectroscopic data described in every group includes corresponding sample to be analyzed in three-dimensional fluorescence
Fluorescence emission wavelengths numerical value corresponding at least one fluorescence spectrum peak in spectrum test process, excitation wavelength numerical value and glimmering
Optical power values;
Component parameter obtains module, for obtaining fluorescence emission wavelengths corresponding at least one Dissolved Organic Matter DOM component
Range and excitation wavelength range;
Image Rendering module is shown, for the correspondence of the spectroscopic data described in every group in the current display picture of the display component
Each fluorescence spectrum peak carry out location point calibration, and at least one of described DOM component progress side in the display picture
Boundary's regional assignment;
Peak detection determining module, for detecting frontier district corresponding to each DOM component at least one of described DOM component
The location point at fluorescence spectrum peak whether is distributed in domain, and determines according to testing result every at least one of described DOM component
Item DOM component matched target fluorescent spectrum peak in corresponding borderline region;
The selection result output module, for the target to each DOM components do match at least one of described in obtaining in DOM component
The fluorescence emission wavelengths numerical value at fluorescence spectrum peak, excitation wavelength numerical value and florescent intensity value carry out data summarization, obtain pair
The fluorescence spectrum peak the selection result answered.
7. device according to claim 6, which is characterized in that there is one with fluorescence emission wavelengths creation in the display picture
And displaing coordinate system of the excitation wavelength as coordinate variable, the display Image Rendering module are specifically used for:
For each fluorescence spectrum peak, according to the fluorescence emission wavelengths of the fluorescence spectrum peak in corresponding three-dimensional fluorescence data
Numerical value and excitation wavelength numerical value obtain the fluorescence spectrum peak corresponding position coordinates in the displaing coordinate system;
The location point at the fluorescence spectrum peak is drawn out in the displaing coordinate system according to the position coordinates at the fluorescence spectrum peak.
8. device according to claim 7, which is characterized in that the display Image Rendering module also particularly useful for:
For each DOM component at least one of described DOM component, according to the fluorescent emission wave of the DOM component got
Long range and excitation wavelength range obtain the DOM component corresponding each extreme coordinates in the displaing coordinate system;
The corresponding boundary of DOM component is drawn out in the displaing coordinate system according to the corresponding each extreme coordinates of the DOM component
Region.
9. device according to claim 8, which is characterized in that the displaing coordinate system is with fluorescence emission wavelengths for horizontal seat
Mark and with the coordinate system of a length of ordinate of excitation light wave, or with a length of abscissa of excitation light wave and with fluorescence emission wavelengths be vertical sit
Target coordinate system.
10. the device according to any one of claim 6-9, which is characterized in that the peak detection determining module tool
Body is used for:
For each DOM component at least one of described DOM component, if the testing result of the DOM component is corresponding frontier district
Without location point in domain, then the target fluorescent spectrum peak of the corresponding DOM component of the borderline region is sky;
If the testing result of the DOM component is that there are a location points in corresponding borderline region, corresponding to the location point
Fluorescence spectrum peak, the target fluorescent spectrum peak as the corresponding DOM component of the borderline region;
If the testing result of the DOM component is that there are at least two location points in corresponding borderline region, out of this borderline region
The corresponding fluorescence spectrum peak of all location points in, choose the maximum fluorescence spectrum peak of florescent intensity value as the boundary
The target fluorescent spectrum peak of the corresponding DOM component in region.
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