CN109725411B - Sample fixing device for dissecting mirror and dissecting mirror - Google Patents

Sample fixing device for dissecting mirror and dissecting mirror Download PDF

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Publication number
CN109725411B
CN109725411B CN201711056894.6A CN201711056894A CN109725411B CN 109725411 B CN109725411 B CN 109725411B CN 201711056894 A CN201711056894 A CN 201711056894A CN 109725411 B CN109725411 B CN 109725411B
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CN
China
Prior art keywords
light
transmitting
sample
dissecting mirror
supporting
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Expired - Fee Related
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CN201711056894.6A
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Chinese (zh)
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CN109725411A (en
Inventor
洪亚平
张有福
陈春艳
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Henan University of Science and Technology
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Henan University of Science and Technology
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Priority to CN201711056894.6A priority Critical patent/CN109725411B/en
Publication of CN109725411A publication Critical patent/CN109725411A/en
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Publication of CN109725411B publication Critical patent/CN109725411B/en
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Abstract

The invention relates to a sample fixing device for an anatomical lens and the anatomical lens, and belongs to the technical field of biological observation equipment. The sample fixing device for the dissecting mirror comprises a light-transmitting disc surface, a sample supporting rubber block arranged on the light-transmitting disc surface and used for supporting and fixing a sample, and a disc surface supporting structure used for supporting the light-transmitting disc surface from the upper surface of a corresponding dissecting mirror base. The sample fixing device for the dissecting mirror supports the light-transmitting disk surface through the disk surface supporting structure and is far away from the upper surface of the dissecting mirror base, so that a certain distance is reserved between the supporting rubber blocks on the light-transmitting disk surface and the upper surface of the dissecting mirror base. The supporting rubber block on the transparent disk surface can be far away from the light source on the base of the dissecting mirror, and the phenomenon of softening or melting of the rubber block is avoided.

Description

Sample fixing device for dissecting mirror and dissecting mirror
Technical Field
The invention relates to a sample fixing device for an anatomical lens and the anatomical lens, and belongs to the technical field of biological observation equipment.
Background
The dissecting mirror is a device commonly used for morphological observation and dissection of plant materials (flowers, seeds, leaves and the like), and the illuminating mode of the dissecting mirror in use generally comprises three modes of reflected light illumination, transmitted light illumination and natural light illumination. Since the transmitted light illumination is a backlight illumination, the observation surface of the plant material is dark and unclear. The working disc of the dissecting mirror in the prior art is generally a circular disc and is arranged in a groove on the upper surface of a base of the dissecting mirror, the height of the circular disc is very small, a sample is placed on the working disc, and the irradiation of indoor weak natural light to the sample is generally weak, so that the indoor observation requirement cannot be met by the natural light illumination. In the prior art, in order to improve the light saturation degree during the observation of a sample, reflected light illumination is generally adopted during the morphological observation and the dissection of a plant material. But it is difficult to satisfy multiple viewing requirements with a single illumination mode.
The Chinese patent with application publication No. CN102870611A discloses a method for observing the flower morphology of a plant, wherein a supporting rubber block is placed in a visual field under a dissecting mirror, and a flower to be observed is bonded at the top end of the supporting rubber block, so that the combined observation of various illumination modes can be carried out.
However, in practice, it has been found that when the rubber block is used for dark field and mixed light illumination observation and photography, the rubber block is too close to the transmitted light illumination light source in the base of the dissecting mirror, so that the observation, dissection or photography takes a long time, and the bright and hot illumination light source can quickly heat up and stick the rubber block to melt, which makes the observation and dissection of the plant material difficult to continue.
Disclosure of Invention
The invention aims to provide a sample fixing device for a dissecting mirror, which has good heat resistance.
The invention also aims to provide a dissecting mirror using the sample fixing device for the dissecting mirror.
In order to achieve the above object, the present invention provides a sample fixing device for a dissecting mirror, comprising:
the utility model provides a sample fixing device for dissecting mirror, includes the printing opacity quotation and sets up and support the sample on the printing opacity quotation and glue the piece to the sample that the sample was supported fixedly, still includes the quotation bearing structure who is used for will printing opacity quotation to prop from corresponding dissecting mirror base upper surface.
The sample fixing device for the dissecting mirror supports the light-transmitting disk surface through the disk surface supporting structure and is far away from the upper surface of the dissecting mirror base, so that a certain distance is reserved between the supporting rubber blocks on the light-transmitting disk surface and the upper surface of the dissecting mirror base. Because the base of the dissecting mirror is provided with the light source, after the dissecting mirror is irradiated by the light source for a long time, the supporting rubber block which plays a role in bonding can be heated and softened or melted, so that the fixing effect of a sample fixing structure or a sample can be influenced, and the observation result can be influenced. The sample fixing device is provided with the disk surface supporting structure, so that the supporting rubber block on the transparent disk surface can be far away from the light source on the base of the dissecting mirror, and the phenomenon of softening or melting of the rubber block is avoided. Adopt to support gluey piece to make the fixed effect of sample more firm on the one hand to the sample is fixed, on the other hand can also make the fixed process of sample simple more convenient, can also adjust the observation gesture of sample under the anatomical lens at any time.
The light-transmitting disc surface supporting structure comprises a light-transmitting supporting frame and a frosted glass sheet arranged on the light-transmitting supporting frame, and the upper surface of the frosted glass sheet forms the light-transmitting disc surface. The setting up of ground glass piece can make things convenient for light to pass through on the one hand, and on the other hand can also disperse the light that the light source sent, makes it more even.
The light-transmitting support frame comprises an inner light-transmitting piece and an outer light-transmitting piece which are sleeved together, and the two light-transmitting pieces are barrel-shaped structures with openings deviating from the ground glass sheet. Preferably, the inner and outer light-transmitting members are two glass culture dishes which are different in size and sleeved together. The large dish fits over the small dish. The openings of the big and small culture dishes are downward.
The inner and outer light-transmitting pieces are respectively provided with a bottom wall and a side wall, and a heat dissipation gap is arranged between the bottom walls and/or between the side walls of the inner and outer light-transmitting pieces. The heat dissipation gap timely dissipates the heat generated by the light source during irradiation, thereby further avoiding the melting of the supporting rubber block on the transparent plate surface.
And a semitransparent plastic sheet is arranged on the outer side surface and/or the inner side surface of the bottom wall of the inner light-transmitting member and/or the outer light-transmitting member. The arrangement of the semitransparent plastic sheet can further disperse the light emitted by the light source of the dissecting mirror, so that the light is more uniform. The translucent plastic sheet is preferably positioned on the outside of the bottom wall of the inner light transmitting member and/or on the inside of the bottom wall of the outer light transmitting member.
The inner light-transmitting piece is provided with a light source accommodating cavity, and an inlet and an outlet for the corresponding light source to enter and exit the light source accommodating cavity are formed in the inner light-transmitting piece. The light source is arranged in the inner light-transmitting piece, and the light source can be used as a standby power supply for illumination when the light source on the dissecting mirror cannot emit light. And illumination can be provided for sample observation when the dissecting mirror is operated in the field.
The sample holding device for the dissector further comprises a light barrier arranged behind the sample in the observation direction. The light barrier can block light rays emitted to the sample from the lower part of the sample on one hand, and can absorb or reflect light rays emitted by a light source arranged above the sample on the other hand. The baffles may also provide a background for the observation of the sample, such as a black background for the sample when the baffles are black baffles. If a background of another color is desired, a light barrier of the corresponding color may be used. The light barrier can be removed when no shading or background is required.
The sample fixing device for the dissector further comprises a sample support frame fixedly arranged on the sample support rubber block, and a fixing rubber block used for bonding and fixing a sample is arranged on the sample support frame. The sample support frame is a sample fixing column or a sample fixing platform. Therefore, more space is arranged around the sample, more sufficient light illumination is provided, the light saturation degree of the sample during observation is improved, and the observed sample image is clearer.
The fixing mode of the sample support frame on the light-transmitting disk surface can adopt various, preferably, the lower end of the sample support frame is bonded and fixed on the light-transmitting disk surface.
In order to support the light barrier, the sample holding device for the dissector further comprises a support arm for supporting the light barrier. One end of the supporting arm is fixedly connected with one end of the light barrier far away from the light-transmitting disk surface, and the other end of the supporting arm is fixedly connected with the light-transmitting disk surface. Preferably, the support arm, the light barrier and the light-transmitting disk surface are fixed by gluing.
In order to reflect the light emitted from the upper part of the sample to other directions and avoid the light from being reflected to the bottom of the sample, the light barrier is obliquely arranged relative to the surface of the light-transmitting disk. In order to adjust the included angle between the light barrier and the light-transmitting disk surface conveniently, the supporting arm is a supporting arm with adjustable length. Preferably, the support arm is a telescopic arm.
The fixing mode of the light barrier can adopt various modes, and generally, the light barrier is fixed on the sample support frame, so that the position relation between the light barrier and the sample can be adjusted. Preferably, the light barrier is provided with a through hole or a notch for the sample support frame to pass through so as to limit the light barrier. When the sample support frame is a sample fixing column, the sample fixing column is arranged in the through hole in the light barrier in a penetrating mode to limit and fix the light barrier. At this point, the light barrier is typically not removed from the sample holding column. When the light barrier is provided with the notch, the sample fixing column can be clamped in the notch, so that the light barrier can be fixed conveniently and can be taken down conveniently. The notch on the light barrier is arranged on one side of the light barrier, the width of the notch is slightly larger than the outer diameter of the sample fixing column, and the length of the notch is about half of the width of the light barrier in the extending direction of the notch, so that the sample fixing column is arranged in the center of the light barrier.
The preparation method of the sample fixing device for the dissecting mirror comprises the following steps: and fixedly connecting the lower end of the sample supporting rubber block with the transparent disk surface to obtain the sample supporting rubber block.
The technical scheme of the dissecting mirror of the invention is as follows:
the utility model provides an dissecting mirror, includes the base and sets up the mirror holder on the base, be provided with the lens cone on the mirror holder, the position that corresponds with dissecting mirror light source on the base is provided with dissecting mirror sample fixing device for the mirror, sample fixing device includes the printing opacity quotation and sets up and supports the sample fixed and support the gluey piece in the printing opacity quotation, still includes the quotation bearing structure that is used for propping the printing opacity quotation from corresponding dissecting mirror base upper surface.
The light-transmitting disc surface supporting structure comprises a light-transmitting supporting frame and a frosted glass sheet arranged on the light-transmitting supporting frame, and the upper surface of the frosted glass sheet forms the light-transmitting disc surface.
Drawings
FIG. 1 is a schematic view of a sample holding device for a dissecting mirror in an embodiment of the dissecting mirror of the present invention;
FIG. 2 is an exploded perspective view of a sample holding device for a dissecting mirror in an embodiment of the dissecting mirror of the present invention;
FIG. 3 is a graph showing the effect of reflected light illumination on embryos isolated from plant seeds using the dissector of the present invention;
FIG. 4 is a diagram showing the effect of dark field observation of embryos isolated from plant seeds using the dissecting mirror of the present invention.
Detailed Description
The following further describes embodiments of the present invention with reference to the drawings.
A specific embodiment of the dissector scope of the present invention is shown in fig. 1 and 2. The dissecting mirror comprises a base and a mirror frame arranged on the base, wherein a lens barrel is arranged on the mirror frame, and a sample fixing device for the dissecting mirror is arranged on a table top of the base. The sample fixing device for the dissector comprises a light-transmitting disk surface, a disk surface supporting structure 1 for supporting the light-transmitting disk surface and a sample fixing structure 2 arranged on the disk surface supporting structure.
The disk surface supporting structure 1 comprises an outer light-transmitting piece 101 and an inner light-transmitting piece 102, the outer light-transmitting piece 101 and the inner light-transmitting piece 102 are both of a barrel structure with an opening at one end, the openings of the outer light-transmitting piece 101 and the inner light-transmitting piece 102 face downwards, and the inner diameter of the outer light-transmitting piece is slightly larger than the outer diameter of the inner light-transmitting piece. The outer light-transmitting piece is sleeved outside the inner light-transmitting piece, namely the inner light-transmitting piece is arranged in the inner portion, and the outer light-transmitting piece is arranged outside the outer portion. The outer side of the side wall of the inner light-transmitting piece is fixedly connected with the inner side of the side wall of the barrel of the outer light-transmitting piece by gluing. Because the internal diameter of outer light-transmitting member is greater than the external diameter of interior light-transmitting member, after gluing, formed the clearance between outer light-transmitting member and the interior light-transmitting member lateral wall, strengthened heat dispersion. A gap is also arranged between the bottom of the outer light-transmitting piece and the bottom of the inner light-transmitting piece, and the heat dissipation performance is further enhanced.
A semi-transparent circular plastic sheet 103 is bonded and laid on the outer side of the bottom wall of the inner light-transmitting member, and the diameter of the plastic sheet is the same as the inner diameter of the inner light-transmitting member. The outside of printing opacity spare diapire can be than putting a little less than the inboard temperature including the setting of circular plastic sheet, prevents that the plastic sheet from being heated and producing the peculiar smell. The circular plastic sheet may be disposed inside the bottom wall of the outer light-transmitting member, or one circular plastic sheet may be disposed outside the bottom wall of the inner light-transmitting member and inside the bottom wall of the outer light-transmitting member.
Specifically, the outer light-transmitting member 101 and the inner light-transmitting member 102 are both glass culture dishes, and three small rubber blocks are uniformly distributed on the side wall of the inner light-transmitting member in the circumferential direction and are bonded with the side wall of the outer light-transmitting member through the three rubber blocks. The diameter of the dish as the outer light-transmitting member was 9.5cm, and the diameter of the dish as the inner light-transmitting member was 9 cm.
A round single-sided frosted glass sheet 104 is fixedly bonded on the top surface of the outer light-transmitting member and extends in a direction parallel to the top surface of the outer light-transmitting member. The smooth surface of the ground glass sheet faces upwards. The upper surface of the ground glass sheet forms a light-transmitting disk surface. The ground glass piece had a diameter of 9.5cm and a thickness of 3 mm. The ground glass sheet is bonded with the top surface of the outer light-transmitting piece through three glue blocks which are uniformly distributed in the peripheral direction of the edge of the top surface of the outer light-transmitting piece. The smooth surface of the single-side frosted glass sheet is over-up, and the frosted surface is downward.
The sample fixing structure comprises a sample fixing column 201, the lower end of the sample fixing column is fixedly arranged on the upper surface of the ground glass sheet, and a sample fixing rubber block 202 is arranged at the upper end of the sample fixing column and used for bonding a sample so as to fix the sample at the upper end of the sample fixing column. In this example, the sample holding column is a toothpick, one end of which is pointed and the other end of which is flat. A supporting rubber block is arranged at the central position of the upper surface of the ground glass sheet, and one end of the toothpick with a sharp head is inserted into the supporting rubber block at the center of the upper surface of the ground glass sheet. A small rubber block, namely a sample fixing rubber block, is arranged at the other end of the toothpick and is used for bonding the sample. The length of the toothpick is about 3.5cm, and the extension direction of the toothpick is vertical to the upper surface of the ground glass sheet.
The upper surface of the ground glass sheet is also provided with a light barrier 3. The light barrier is obliquely arranged relative to the upper surface of the ground glass sheet, and the included angle between the light barrier and the upper surface of the ground glass sheet is about 30 degrees. A notch 301 is arranged at one end of the light barrier which is obliquely arranged and close to the upper surface of the ground glass sheet, the width of the notch is slightly larger than the diameter of the toothpick, and the toothpick is just clamped in the notch on the light barrier. The end of the light barrier remote from the upper surface of the ground glass sheet is connected to a light barrier support structure 302. In this embodiment, the light shielding plate supporting structure is a toothpick as a supporting arm. The toothpick as the supporting arm is arranged in a direction vertical to the upper surface of the ground glass sheet in an extending mode. One end of the toothpick close to the upper surface of the ground glass sheet is bonded on the upper surface of the ground glass sheet through the rubber block, and the other end of the toothpick is bonded on the lower surface of the light barrier through the rubber block so as to support the light barrier and enable the light barrier to keep an inclination angle. One side of the light barrier close to the ground glass sheet is bonded on the supporting rubber block.
The light barrier may also provide a black background for sample viewing, in which case a black light barrier is used. If the sample observation background needs to be set to other colors, only the light barrier with the corresponding color needs to be replaced. When the black or other color background is not needed, the light barrier is taken down. Generally, the light barrier is made of a paperboard or a plastic plate.
The supporting rubber block and the fixing rubber block can be rubber blocks made of gum water washed and placed for several months, and can also be latex blocks in the prior art.
When the dissecting mirror is manufactured, three small rubber blocks are bonded on the outer edge of the bottom wall of a small glass culture dish serving as an inner light-transmitting piece, semi-transparent plastic paper is bonded on the outer side of the bottom wall, then the small glass culture dish is placed into a groove of a dissecting mirror working plate in a downward inverted manner, the three rubber blocks are bonded on the edge of the groove, the small glass culture dish is fixed, the three rubber blocks are bonded on the outer side wall of the small glass culture dish, a large culture dish is sleeved on the small culture dish, and bonding and fixing are achieved. Then, a single-sided ground glass sheet is adhered to the outer surface, namely the upper surface, of the bottom of the large culture dish, a rubber block is arranged in the center of the ground glass sheet, the lower end of a toothpick serving as a sample fixing column is inserted into the rubber block, and the upper end of the toothpick is also provided with the rubber block. Then the notch on the light barrier is clamped into the middle part of the toothpick, and a supporting arm is arranged on the light barrier.
When the dissecting mirror of this embodiment is used, place dissecting mirror sample fixing device on dissecting mirror's base mesa earlier, then bond fixed sample on the fixed glue piece of sample fixed column upper end, can utilize dissecting mirror to observe after fixing the barn door. The observation is a reflected light illumination mode, and when dark field observation is needed, the light barrier can be taken down. The observation images of the embryo separated from the persimmon seed by the dissecting mirror of this example are shown in fig. 3 and 4, in which fig. 3 is the observation effect of the reflected light illumination using the black light barrier, and fig. 4 is the observation effect of the dark field with the light barrier removed.
In other embodiments of the dissector mirror of the present invention, a light source is disposed in the inner light-transmitting member of the sample holding device for the dissector mirror, and the light source is capable of emitting upward light to illuminate the sample after passing through the ground glass sheet. The light source can adopt the LED lamp, and the power of light source can set up inside the interior printing opacity piece. The corresponding positions of the side walls of the inner light-transmitting piece and the outer light-transmitting piece can be provided with through holes or gaps, and a power line of an external power supply penetrates into the cavity of the inner light-transmitting piece through the through holes or the gaps to provide electric energy for the light source. The external power supply can be connected with the mobile power supply through a USB connector, so that the dissecting mirror can be conveniently used in the field or places inconvenient for power taking.
In other embodiments of the anatomical lens of the present invention, the disk support structure consists of only the inner light transmissive member, the outer light transmissive member, and the frosted glass sheet, excluding the plastic sheet in the inner light transmissive member. Or only consists of the outer light-transmitting member and a ground glass sheet arranged on the outer light-transmitting member. Or, only one transparent plastic circular table.
In other embodiments of the dissector mirror of the present invention, the light barrier of the sample holding device is positioned parallel to the upper surface of the ground glass sheet, which provides only a black background for sample observation. The light barrier may not be provided, and the corresponding support arm for supporting the light barrier may not be provided. In other embodiments, the light barrier is centrally provided with a through hole through which the light barrier is sleeved on the sample holding column, and at this time, the light barrier is not generally removed from the sample holding column.
In other embodiments of the dissecting mirror of the present invention, the sample fixing column is replaced by a metal fixing rod, and the fixing manner of the metal fixing rod and the upper surface of the ground glass sheet can adopt the following manner besides gluing: a blind hole with an upward opening is arranged at the center of the ground glass sheet, and the inner diameter of the blind hole is the same as the outer diameter of the metal fixing rod. The metal fixing rod is inserted into the blind hole and is fixed by means of the friction force between the metal fixing rod and the hole wall. The upper end of the metal fixing rod can still adopt a rubber block to fix the sample. A groove can be formed in the upper end of the metal fixing rod, and a sample can be placed in the groove for observation. The metal fixing rod can also be arranged into a telescopic metal rod for adjusting the distance between the sample and the base of the dissecting mirror.
In other embodiments of the dissecting mirror of the present invention, the size of the supporting rubber block disposed in the center of the ground glass surface may be set as desired, and the supporting rubber block may be set to a rubber block with a larger volume so that the lower ends of the toothpick serving as the sample fixing column and the toothpick serving as the supporting arm can be simultaneously bonded thereto.
In other embodiments of the dissector scope of the present invention, the support arm is replaced with a telescoping rod that can telescope in the up-down direction to change the length, such that the angle between the light barrier and the upper surface of the ground glass sheet can be changed as desired. Or a plurality of support arms of different lengths may be provided for replacement.
The sample fixing device for the dissecting mirror in the embodiment of the present invention has the same structure as the sample fixing device for the dissecting mirror in the above-described embodiment of the dissecting mirror, and details thereof are not repeated. The various alternative forms of the sample holder for a dissecting mirror described in the embodiments of the dissecting mirror described above are also suitable for the embodiments of the sample holder for a dissecting mirror of the present invention.

Claims (7)

1. A sample fixing device for a dissecting mirror comprises a light-transmitting disk surface and a sample supporting rubber block which is arranged on the light-transmitting disk surface and used for supporting and fixing a sample, and is characterized by further comprising a disk surface supporting structure used for supporting the light-transmitting disk surface away from the upper surface of a corresponding dissecting mirror base;
the tray surface supporting structure comprises a light-transmitting supporting frame and a frosted glass sheet arranged on the light-transmitting supporting frame, and the upper surface of the frosted glass sheet forms the light-transmitting tray surface;
the light-transmitting support frame comprises an inner light-transmitting piece and an outer light-transmitting piece which are sleeved together, and the two light-transmitting pieces are barrel-shaped structures with openings deviating from the ground glass sheet.
2. The specimen holding device for a dissecting mirror according to claim 1, wherein the inner and outer light-transmitting members each have a bottom wall and a side wall, and a heat dissipation gap is provided between the inner and outer light-transmitting members and/or between the side walls.
3. The specimen holding device for a dissecting mirror according to claim 1, wherein a translucent plastic sheet is provided on an outer side surface and/or an inner side surface of a bottom wall of the inner light-transmitting member and/or the outer light-transmitting member.
4. The sample fixing device for the dissecting mirror as claimed in claim 1, wherein the inner light-transmitting member has a light source accommodating cavity, and an inlet and an outlet for the corresponding light source to enter and exit the light source accommodating cavity are provided on the inner light-transmitting member.
5. The sample holding device for a dissecting mirror as claimed in any one of claims 1 to 4, wherein the sample holding device for a dissecting mirror further comprises a light barrier disposed behind the sample in the observation direction.
6. The sample fixing device for the dissecting mirror as claimed in any one of claims 1-4, wherein the sample fixing device for the dissecting mirror further comprises a sample support frame fixedly arranged on the sample support rubber block, and a fixing rubber block for adhering and fixing a sample is arranged on the sample support frame.
7. A dissecting mirror comprises a base and a mirror frame arranged on the base, wherein a lens barrel is arranged on the mirror frame, and a sample fixing device for the dissecting mirror is arranged on the base at a position corresponding to a light source of the dissecting mirror;
the tray surface supporting structure comprises a light-transmitting supporting frame and a frosted glass sheet arranged on the light-transmitting supporting frame, and the upper surface of the frosted glass sheet forms the light-transmitting tray surface;
the light-transmitting support frame comprises an inner light-transmitting piece and an outer light-transmitting piece which are sleeved together, and the two light-transmitting pieces are barrel-shaped structures with openings deviating from the ground glass sheet.
CN201711056894.6A 2017-10-27 2017-10-27 Sample fixing device for dissecting mirror and dissecting mirror Expired - Fee Related CN109725411B (en)

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CN201711056894.6A CN109725411B (en) 2017-10-27 2017-10-27 Sample fixing device for dissecting mirror and dissecting mirror

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Application Number Priority Date Filing Date Title
CN201711056894.6A CN109725411B (en) 2017-10-27 2017-10-27 Sample fixing device for dissecting mirror and dissecting mirror

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CN109725411B true CN109725411B (en) 2021-01-01

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Publication number Priority date Publication date Assignee Title
CN101792731A (en) * 2010-01-20 2010-08-04 河南科技大学 Method for separating Chinese white poplar ovule from nucellus
CN102870611A (en) * 2012-09-10 2013-01-16 河南科技大学 Method for observing morphology of plant flower
CN107144952A (en) * 2017-07-18 2017-09-08 深圳小孚医疗科技有限公司 Micro imaging system for sample analysis

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Publication number Priority date Publication date Assignee Title
CN101792731A (en) * 2010-01-20 2010-08-04 河南科技大学 Method for separating Chinese white poplar ovule from nucellus
CN102870611A (en) * 2012-09-10 2013-01-16 河南科技大学 Method for observing morphology of plant flower
CN107144952A (en) * 2017-07-18 2017-09-08 深圳小孚医疗科技有限公司 Micro imaging system for sample analysis

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在解剖镜下利用胶块观察植物花形态的方法;洪亚平 等;《安徽农业科学》;20080801;第22卷(第36期);第9482、9483、9539页 *

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